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quality assurance/quality control manual for the lower cape fear river

1. Target Reporting Level Comments x Resolution to 0 1 degree Celsius Reportrsulstothenearest mg l Meters should be calibrated to measure a pH range of at least 4 01 to 9 18 Report results to the nearest 0 1 pH units Specific Conductivity Report results to the nearest whole uS cm at 25 C LCFRP MOA Page 10 of 17 Table B 1 Continued DWO Laboratory Reporting Limits and Methods _ Parameters Target Reporting Level a a x Comments Turbidity 1 0 NTU S 6 2 mg L Entercocci UN At least 3 dilutions should be used to achieve optimum colony counts per membrane filter of 20 60 colonies Fecal Coliform 1 colony 100 mL Report Chlorophyll a values free from pheophytin and other chlorophyll pigments Not listed 1n 40 CFR 136 Analysis by HPLC is not approved by DWO Address distillation requirement See 40CFR136 Table II footnote Chlorophyll a 1 pg L Ammonia NH3 as N Nitrate Nitrite as 0 02 mg L 0 02 mg L Total Kjeldahl Nitrogen as N Total Phosphorus 0 20 mg L as P 0 02 mg L x A Oe LI ET GE EE EE A A C AR LI Fe sugL 5 0 0 0 0 Pb LI lOugL Le O Hg O O2pgL LI x N Jugi 1 O i 10 ug L SM Standard Methods for the Examination of Water and Wastewater 18 19 and 20th ed EPA EPA Method see 40 CFR 136 http www gpoaccess gov cfr index html APHA American Public Health Associat
2. meme e cmm _ Appendix V LCFRP Subscribers Lower Cape Fear River Program Subscriber Contact Information Updated April 2014 Dr James Merritt UNCW Center for Marine Science Program Coordinator 910 962 2303 Dr Mike Mallin Aquatic Ecology Laboratory UNCW CMS Research Coordinator 910 962 2358 Matthew Mclver Aquatic Ecology Laboratory UNCW CMS Sampling Coordinator 910 962 2357 Ed Kreul International paper Technical Committee Chair 910 362 4883 Shawn O Connor Global Nuclear Fuels QA QC Committee Chair 910 819 5869 is 2 Signature Facility Permit Title Contact Person email Address Authority Lisa Osth City of Clinton City of Clinton NC0020117 Neill Carroll Wastewater Treatment Manager ROA 910 299 4912 P O Box 199 LisaoOcityofclintonnc us i Clinton N C 28329 0199 CFPUA CREUS NC0023965 Matthew W Jordan CFPUA General Manager Bem bere 910 332 6646 235 Government Center Drive Northside WWTP beth eckertO cfpua org SES Wilmington NC 28403 CFPUA EUA NC0023973 Matthew W Jordan CFPUA General Manager Bri Por 910 332 6646 235 Government Center Drive Southside WWTP beth eckert cfpua org ae Wilmington NC 28403 Elizabeth Wike DAK Americas LLC DAK Americas LLC NC0000663 Penny Mahoney Operating Director A 910 371 4498 3500 Daniel Road SW ewike dakamericas com Le
3. Analytical Methods and SOPs I Laboraotry Analytical Methods A Environmental Chemists Inc Analyses and Methods 6602 Windmill Way Total Phosphorus SM 4500 P E 1999 Wilmington NC 28405 Kjeldahl Nitrogen EPA 351 2 Rev 2 0 1993 910 392 0223 Nitrite Nitrate EPA 353 2 Rev 2 0 1993 Lab Certification 94 Total Sus Solids SM 2540 D 1997 Fecal Coliform SM 9222 D 1997 Enterococcus Enterolert IDEXX Lab Turbidity SM 2130 B 2001 B Environment 1 Inc Analyses and Methods 114 Oakmont Drive Ammonia Nitrogen EPA 350 1 Rev2 0 1993 Greenville NC 27858 252 756 6208 Lab Certification 10 C Aquatic Ecology Laboratory Analyses and Methods UNCW Center for Marine Sci Chlorophyll a EPA 445 0 Rev 1 2 5600 Marvin Moss Lane Temperature SM 2550 B 2000 Wilmington NC 28409 Dissolved Oxygen SM 4500 O G 2001 910 962 2359 Specific Conductivity SM 2510 B 1997 Lab Certification 638 pH SM 4500 H B 2000 II Specific lab Standard Operating Procedures for each method can be obtained by emailing a request to Matthew McIver mciverm uncw edu in the Aquatic Ecology Laboratory
4. Composite or Grab MN Container S 4 u MN Chlorine z Y G P A 1 Temperature when Received Accepted __ Rejected Resample Requested Delivered By 8 Received By Date CTs Comments m Section 7 Page 3 of 4 Version 6 May 2014 COC 1 2 1 Chlorophyll a Chain of Custody for Grinding Analysis Samples LCFRP Instructions Initials Date and Time are required for each action Collected By Date Time Received on Ice Duplicates Yes or No Filtered By Filters m Freezer time Section 8 Page 1 of 5 Version 6 May 2014 Section 8 Multi Parameter Water Ouality Meter Operation A Multi Parameter Water Quality Meter MPWQM consists of a hand held display unit a cable and a sonde equipped with parameter specific probes Each probe 1s calibrated before sampling activities and checked upon return to the laboratory according to procedures outlined by the NC DWR Laboratory Certification program and the Monitoring Coalition Program Field Monitoring Guidance document from the North Carolina Division of Water Resources Coalition Monitoring Program http portal ncdenr org web wq ess eco coalition Calibration data is recorded on a calibration worksheet and kept on file 1n the laboratory attached to the field data sheets Backup meters carried in the field in previous years are no longer used These instruments due to their rare use were usually un reliable If the MPWQM malfunctions in the f
5. LCFRP Advisory Board Tom Wolfe New Hanover Co Commissioner 230 Government Ctr Drive Suite 175 Wilmington NC 28403 910 228 8569 twolfe nhcgov com Myrle Beringer Duplin County 512 Forrest Lane Ext Wallace NC 28466 910 285 5215 cjb1 gsiwave com Don Betz Lower Cape Fear Water amp Sewer Authority Executive Director PO Box 1673 Wilmington NC 28402 910 383 1919 Fred McCoy Pender County Board of Commissioners 362 Harrell Road Burgaw NC 28475 910 259 9349 Fmccoy1 ec rr com Kent Tyndall Duke Energy Progress 1451 Military Cutoff Road Wilmington NC 28403 910 343 3244 Rodney tyndall pgnmail com Donna Lazzari Invista S aR L EHS Manager 4600 Hwy 421 North PO Box 327 Wilmington NC 28402 910 341 3146 Calvin Overcash Elementis Chromium LP 5408 Holly Shelter Rd Castle Hayne NC 28429 910 675 7222 kevin cowan elementis com Jim Durham International Paper 865 John L Riegel Road Riegelwood NC 28456 910 362 4748 jim durham ipaper com Mike Giles NC Coastal Federation Cape Fear Coastkeeper 131 Racine Drive Suite 101 Wilmington NC 28403 910 790 3275 coastkeeper cf nccoast org Dr Richard Huber UNCW Watson School of Education 601 S College Road Wilmington NC 28403 910 962 3561 huberr uncw edu Huey Marshall Brunswick County PO Box 249 Boliva NC 28422 910 253 2019 Greg Martin Bladen County Manager s Office PO Box 1048 Elizabethtown NC 28337 910 862 6700 c
6. LCFRP PERMITTEE SIGNATURES Permittee NPDES Number DAK Americas LLC NC0000663 Invista S AR L NC0001112 Global Nuclear Fuels NC0001228 Americas Carolina Power and Light CP amp L d b a Progress Energy Carolinas Inc NC0001422 Sutton Steam Electric Plant International Paper Company NC0003298 Riegelwood Mill LCFRP MOA Signature Penny Mahoney Operating Director Rick Bayless Environmental Health and Safety Manager Shawn O Connor Environmental Specialist ark Frederick Plant Manager Edward Kreul Manager Environment Health Safety and Sustainability Page 4 of 17 LCFRP PERMITTEE SIGNATURES Permittee NPDES Number Signature DAK Americas LLC NC0000663 Penny Mahoney Operating Director Invista S R L NC0001112 Rick Bayless Environmental Health and Safety Manager Global Nuclear Fuels NC0001228 Americas Shawn O Connor Environmental Specialist Carolina Power and Light CP amp L d b a Progress Energy Carolinas Inc NC0001422 Mark Frederick Sutton Steam Electric Plant Plant Manager International Paper Company NC0003298 VEN AR C Riegelwood Mill amp reu Manager Environment Health Safety and Sustainability LCFRP MOA Page 4 of 17 Momentive Specialty Chemicals Elementis Chromium LP Town of Mount Olive Mt Olive WWTP Town of Burgaw Burgaw WWTP Town of Warsaw Warsaw WWTP Town of Carolina Beach Carolina Beach WWTP Cape Fear Public Utility Authority Northside WWT
7. aimjesoduia y epnjour sjusurounseojA PIAA DES BK QN unda M poysiojem ur sdo 3op pue AESOUION JU 06 I SO 000t0t0 6I FZ 81 OWM H 9158 LL 1870 st BV YAO AN 9 qui fe ON pue T ON Je dures u usoD 00016168 u L000c0t0 _ e OAM uidaq 9 L8L SPELT Se amp HEA Avg sup py sso99e uosie du 3991 uoueag Lomp 0000 16d 8l AayeA Avg woy e dureg 19 EOE A AO a uis jueiquie OMA sure EN L000 0 0 er OWA dnd gei TSLUSE Aua opo sua au cop On wan eo ode gn FOE 00006068 mE RT uou My iu pu g 3ous sioH r3 S000 0 0 12 81 OWM DIE 8696 LL LEHTPE ou o4 ode FN sdn E EE S000 0 me Ct 00105064 L 2 A c00 06d Uu INO 0 _ TER Ad pu puero 5puiq YY CUN as sooosoeo LEFT OWM MSU 2286 22 POST vt pue ASiouq ss313014 sup JO sup ESSELEN Je JOATY 1891 adep EN S eg UOJHO Xiuoouq 2 INSC IN MSO SO00 0 0 9 81 OMIM AdIMsSUNIZ E10 8L IZO ve pue 4Svg yya suq Ju yaoi uerpu sdn Jaary 1894 ode 0000t06d J AI 9000 0t0 89 81 OMIM ES IFFI gi ETS 189 ode jo ie oSpuq I Jig uns J OLT ON Ie Pay pela otza 00000064 1 HHHHHHHHHHHH z Un Us ENLIDDOOOE pousioje ur sdo Zou p o3u WOH mn 9000 0 0 1 89 81 Odd ES 69ST 8L Ip9p pE poysiayem ul suone1edo 30H Aj 0 Je S ON JE 3991 AED T 00018688 MUYO E SOS poysiojem ur suongiodo pjosuy INOd MOSS 9000c0t0 O4 osas cli
8. A group representing industry local and state government and Chancellor Leutze from UNCW proposed an alternate plan This plan proposed that the Cape Fear River Program be implemented as an alternative to the High Quality Waters Classification The Environmental Management Commission accepted this plan and delayed any classification decisions Monitoring programs in the upper began in January 2000 and middle began in 1999 basins of the Cape Fear River have also been established In order to distinguish the effort in the lower basin the name of the program was changed to the Lower Cape Fear River Program in 1998 Section 2 Page 2 of 2 Version 6 May 2014 3 Mission Statement The mission of the Lower Cape Fear River Program is to develop an understanding of processes which control and influence the Cape Fear River and to provide a mechanism for information exchange and public education A Specific Goals Develop implement and manage a basin wide coordinated physical chemical and biological water quality monitoring program Point non point and naturally occurring sources will be considered in developing the monitoring plan Interact with regulatory agencies academic institutions local industries and other groups to determine additional studies and analysis needed to develop an effective and successful management plan Initiate the studies and assist in securing funding to conduct the research Develop scientific information to pr
9. Brunswick Regional Water amp Sewer H2GO Belville WWTP Brunswick County NE Brunswick Regional WWTP LCFRP MOA NC0023973 NC0026018 NC0039527 NC0075540 NC0086819 Marty Lav ing Matthew W Jordan General Manager Kenneth Smith Mayor Matthew W Jordan General Manager Carl Antos Chairman County Manager Page 6 of 17 Table 1 LCFRP Permittees ee RAUS hip and Fa E NC0000663 DAK Americas LLC NC0001112 Invista S R L Penny Manoney Brunswick Wilmington 030617 03030005 Operating Director Rick Bayless Environmental Health and Safety New Hanover Wilmington 030617 03030005 Manager NC0001228 SES E Ge anann Q EE I New Hanover Wilmington 030617 03030007 Americas Environmental Specialist Carolina Power and Light ER Ee New Hanover Wilmington 030617 03030005 Carolinas Inc Plant Manager Sutton Steam Electric Plant International Paper Compan GE U 1 rd Manager Environment Health Columbus Wilmineton 030617 03030005 Riegelwood Mill Nae pet Safety and Sustainability NC0003395 dee Re Columbus Wilmington 030617 03030005 Chemicals Environmental Engineer NC0003875 Elementis Chromium LP ee New Hanover Wilmington 030623 03030007 Vice President Technical Town of Mount Olive Charles Brown Town of Burgaw Kenneth Cowan n Town of Warsaw J R Steigerwald P Town of Carolina Beach Tim Owens ET NC0001422 NC0003298 Cape Fear Public Utility NC0023965 Authority Do i
10. Edward Kreul Manager Environment Health Safety and Sustainability Page 4 of 17 LCFRP PERMITTEE SIGNATURES Permittee DAK Americas LLC Invista S aR L Global Nuclear Fuels Americas Carolina Power and Light CP amp L d b a Progress Energy Carolinas Inc Sutton Steam Electric Plant International Paper Company Riegelwood Mill LCFRP MOA NPDES Number NC0000663 NC0001112 NC0001228 NC0001422 NC0003298 Signature Penny Mahoney Operating Director am eras Envirenmental Health and Safety Manager Sire MAniGer p Shawn O Connor Environmental Specialist Mark Frederick Plant Manager Edward Kreul Manager Environment Health Safety and Sustainability Page 4 of 17 LCFRP PERMITTEE SIGNATURES Permittee NPDES Number Signature DAK Americas LLC NC0000663 Penny Mahoney Operating Director Invista S R L NCO0001112 Rick Bayless Environmental Health and Safety Manager Global Nuclear Fuels NC0001228 lia x Milk Hal aes Americas Shawn O Connor Environmental Specialist Carolina Power and Light CP amp L d b a Progress Energy Carolinas Inc NC0Q001422 Mark Frederick Sutton Steam Electric Plant Plant Manager International Paper Company NC0003298 Riegelwood Mill Edward Kreul Manager Environment Health Safety and Sustainability BI ze sis e E k E PAS Gees 3 Des gie JUN 1 0 201 Environmental Sciences Section LCFRP MOA Page 4 of 17
11. GE New Hanover Wilmington 030617 03030005 Northside WWTP SE Cape Fear Public Utility NC0023973 Authority SCC ge Mss New Hanover Wilmington 030617 03030005 Southside WWTP Town of Beulaville Kenneth Smith I e Cape Fear Public Utility Authority Matthew W Jordan TT Walnut Hills Subdivision General Manager SE SE SEN Ee WWTP Brunswick Regional Water amp Qu kutoa Sewer H2GO p Brunswick Wilmington 030617 03030005 Belville WWTP Brunswick County NE Brunswick Regional Mary awing Brunswick Wilmington 030617 03030005 WWTP County Manager NC0039527 NC0075540 NC0086819 LCFRP MOA Page 7 of 17 L130 8 93B4 VOW d AOT dd oui pue OMA Jo lu uu i8g og ye umpueaouroui o 0z Udy s OMA 12d papuadsns sem 3uuojruoul s BJ JA S ejout feo 10j 3q I ses eue IV uz ouz pue IN I933IN SH Amora qq pee 04 gout 19 saddo 17 umiuon pO umnupeg sy 3ru siy y umurum y spejau BUIMO JOF 21 107 120u1229 1 pue 1990 5 sunny ounf Judy Axeniqo 4 uonoojoo duigs yuo 1900 Ain TO Surduies SeN 41 d se stuoydsoyg 830 1 pue NL UJN Yepp BIOL EON ZON N SE 9JLYIN AIBMIN EHN N Se piuoutury s pnj ui Suijduieg juougnw osue suonipuoo gugenuojxo usyM jdooxo pede s ep ud jse2 je po129 oo 3q o oue so duies AjyJUOU DIM 1oquiojdog pue YsnBny Anpe ouny Ae jo sujuoui ap sopnjour sous durdures rouiuins Ajqguour 99149 qr AppuojA JASCHN Apun GQiano npuoo pue Hd uss x paajossig
12. gt 6 C during transport or not verifiable eg no temperature blank provided non reportable for NPDES compliance monitoring J6 The laboratory analysis was from an unpreserved or improperly chemically preserved sample The data may not be accurate J7 This qualifier is used to identify analyte concentration exceeding the upper calibration range of the analytical instrument method The reported value should be considered estimated J8 Temperature limits exceeds samples frozen or gt 6 C during storage The data may not be accurate JO The reported value is determined by a one point estimation rather than against a regression equation The estimated concentration is less than the laboratory practical quantitation limit and greater than the laboratory method detection limit J10 Unidentified peak estimated value J11 The reported value is determined by a one point estimation rather than against a regression LCFRP MOA Page 13 of 17 Data Remark Code Definition equation The estimated concentration is less than the laboratory practical quantitation limit and greater than the laboratory method detection limit This code is used when an MDL has not been established for the analyte in question 112 The calibration verification did not meet the calibration acceptance criterion for field parameters Note A J value shall not be used if another code applies ex N V M Sample and duplicate results a
13. possibly a result of incomplete cleaning of the DO probe between samples Dissolved Oxygen Meter Air Calibration stopper BOD bottle used for calibration and shake vigorously to saturate the air inside Gently dab membrane on BOD oxygen probe and place in calibration bottle Using the menu at the bottom of the screen select Calibrate then DO Cal Select Next twice until salinity is blinking adjust salinity to 0 0 Select Next once and barometric pressure will blink Enter the correct barometric pressure using lab barometer Press the enter button and the values will be saved and instrument will return to calibration mode 10 Allow probe 15 minutes to warm up and equilibrate This is a good time to measure pH 11 When the DO is stable select Auto Cal 12 DO is now calibrated return to main display by pressing Mode 13 DO probe membranes should be replaced every other month Record date of change in YSI V Calibration Log pH Meter Calibration and Measurement Fisher Scientific Accumet Basic Initial 5 day and 20 day pH should be measured for each sample l 2 3 4 Plug the meter in to turn it on Press pH mV button until pH reads on the screen We calibrate the meter at 7 0 and 4 0 since this brackets most of our sample pH values Press Setup and Clear Buffers will blink press Enter The meter will go back to pH display and show Measurement at t
14. April 30 for each year the Agreement is in effect The annual report will summarize all data collected in the past calendar year and contain the following elements e Monitoring Station List to include station number station description county accurate coordinates in decimal degrees to 4 decimal places using NAD83 stream classification and 8 digit hydrologic unit code HUC e List of all certified laboratories that conducted work for the coalition in the past year and laboratory methods used for all parameters Summarize any laboratory certification issues for individual parameters e Submit a CD that includes all monitoring data for the past year with a statistical summary for each station These data should be combined into a single table containing the year s reviewed and finalized data which may be placed on the DWQ web site The annual statistical summary must describe for each parameter at each location o Number of observations N o Number of observations less than the laboratory reporting level N lt RL o Identify the water quality standard action level or other reference level Ref o Identify the number of observations that do not meet the reference level N gt Ref or N lt Ref o Maximum observed value and Minimum observed value o Annual arithmetic mean use a geometric mean for fecal coliform data e Include a list of active LCFRP members with authorized representative updates contact names email addresses and p
15. EE Downstream E wan 34 3637 71 8965 BSw 0303007 Castle Hayne of point source discharges SC CH B9720000 Smith Creek at Us 117 and NC 133 at pow runoff Dosnsueam of New Hanover 34 2586 71 9391 CSw 0303007 Wilmington Wilmington Northside WWTP SS Section 5 Page 3 of 3 Version 6 May 2014 Figure 5 1 LCFRP Station Map Lower Cape Fear River Program Monitoring abone AR ETT SR eer 403 LENOIR E d aison o A Fayetteville E r Clinton GS ad E E i y ber JONES CUMBERLAND amp SAMPSON SAR on ES DUPLIN gt 1 e GCO tt i S k eet LEO j a l RC Wallace L m E Ta Ze ROBES N Eliza cdi own es S Em j BLADEN KE Mood E Si A TY ed i HAM PENDER 9 As i e Burgaw o d od Ka e r OL z Fen CU Deme Sich NCF117 w e e PIC NCF6 SE LVC2 DP x SCC AN A e LCFRP Stations Rivers County Boundaries BRUNSWICK Municipalites Cape Fear River Basin Miles O 4 8 16 Section 6 Page 1 of 7 Version 6 May 2014 Section 6 Standard Operating Procedures for Field Sampling Water samples are collected and delivered to a state certified laboratory to be analyzed for fecal coliform enterococcus bacteria heavy metals total suspended solids lab turbidity and nutrients MOA required chlorophyll a samples are analyzed in the AEL using NC DWR certified procedures Non MOA required chlorophyll a and Biochemical Oxygen Dema
16. LO S Iu HHH gud x 0 0 u mama ESTAN Edd 2 uonus wonus Lann Ka AMAT Kai eg 2 eU P Kouoanbou q Zuuduge pue s 1939018 18 suong SurldurgS duo T I V Ade NV Id DNBHIO LINOWN dY V XIGNHddV L1Jo 6 33ed VOW dY491 TAIDOT Ap pue OMA JO Ju9ur mde oy ye umpugaoureur 0102 Udy s OMq Jed papuadsns SEM JUHOMUOW s p ojA s g uu 030 10 9q Uia sosA eue UN UZ ourz pue IN MIN 3H Alma qq peog 94 uoy no saddo 19 unnuoryo po unnupeo sy S3ru siv py wunurun y spejour Jumojjoz ou 1037 19quiSYag pue i qo poO 9saBny aunt judy Asenigs J uouoo oo ajduies qjuojq 190 A19 3 JAO Suiduies sN dL d Se snioudsoud eo L pue NL u 8oniN epy 820 EON ZON N s JHHN JEBIN EHN N S truoumury sapnjour Zutdugg MN SL suonipuoo S uugnu x uo jdooxo pede sep uo jseo J pojoo oo aq o1 oue sopduies gyuow 9214 Jeoqui d s pue 9sn3ny Amt ounf ABI Jo sujuout oY s pnjout Iwung Suipduies 1oununs Jyjuoul 3144 YM A uguojAj JASZ IN Allen QiAnonpuoo pue Hd usSAx poajossiq ounje1eduio g IPN OUI sjusurounseoJA PJA I a Ce Im T ae IATA 81 Se MOH usd 628 vin l ET See etd z uy E RIOEN T EE St ms nl a iw os sooososo aasi ours xusumg oseese seeoye faLMa yoved vunowo sd uq
17. NCAC 2H 0800 is pleased to renew certification for your laboratory to perform specified environmental analyses required by EMC monitoring and reporting regulations 15 NCAC 2B 0500 2H 0900 and 2L 0100 0200 0300 and 2N 0100 through 0800 Enclosed for your use is a certificate describing the requirements and limits of your certification Please review this certificate and attachment to insure that your laboratory is certified for all parameters required to properly meet your certification needs Please contact us at 919 733 3908 if you have questions or need additional information Enclosure DENR DWR WQP Laboratory Section NC Wastewater Groundwater Laboratory Certification Branch 1623 Mail Service Center Raleigh North Carolina 27699 1623 Location 4405 Reedy Creek Road Raleigh North Carolina 27607 6445 Phone 919 733 3908 FAX 919 733 6241 Internet www dwalab org An Equal Opportunity Affirmative Action Employer Appendix VIT continued vLOZ LE IZL vLO L L HCH ejeq uoneJdx3 ejeg 9519943 JOQUINN APAD 2080 HZ OVON VSL U YHO jas se suonoeJjul JO uoneonueoep JO pue senjeued io o el ng ee seuojeJoqe sejduies uonenje e uo soueuojed loj0ejspes pue ABojopoujew panoldde jo esn weiGosd eoueunsse Ayjenb ejqejdeooe ue jo e uejureui selnbas uoneognieo siu snoenby 000Z 8 0SSz WS J4NIVIITANAL snoenby 000Z 8 H 00Sr WS Hd snoenby 100Z 9 O 00Sr INS N39AXO 0UJATOSSIO snoen
18. Utilities Authority 235 Government Center Drive Wilmington NC 28403 910 332 6586 Ken vogt cfpua org Appendix IV LCFRP Technical Committee Name Affiliation Title Address Phone Email C ema ovas O OO omoa osan aeoo BREET e De Miims Ca CC TA mne oaeo oaeen CO TT eese eme eegene omina ees MET E OATES ae PET neue pco os JO rar menea _ IET ATT ascensor De Mies CEPA DET ETT ascensor De Mimes ETT osas tee veo com ono o encina aneron MT E ATC Jas Les wat TP MENTA TEN wee comem OOO G oncer O ETT C seos ommon TAC oessa TT egen Comae MENTA TEO peres enerom AAA mosca semen BREEDE REENEN some Lasel o ome nCman meng Lesen asas TP eneen esses ECT meses Leen _ C seas meenam 0 oonan 9emum Appendix IV continued Name Affiliation Title Address Phone Email Kent Tyndall Duke Energy Progress QE 801 Sutton Steam Plant Rd Wilmington NC 28401 910 343 3244 rodney tyndall pgnmail com Cape Fear Public Utility Auth ws 235 Government Ctr Dr Wilmington NC 28403 910 332 6736 ken vogt cfpua org Todd Walton NC Ports Authortty W sss PO Box 9002 Wilmington NC 28402 910 251 5678 todd_walton Oncports com i wanes DETECTAN meras ANTEC moss ECT REENERT C aws CEI ceaun ee wangon ncasa oorsese iavaran rana saco
19. Wrap the filters together in a big piece of foil and label the outside with place of collection date and amount filtered Place this in the freezer in an air tight plastic container with anti humidity material desiccant 13 The temperature of the freezer should be recorded once per 24 hours during storage on logsheet LS 1 2 1 14 Clean the filter apparatus with a hot water rinse and finish with a DI water rinse Extraction of Chlorophyll a 1 Maximum hold time for frozen filters 1s four weeks 2 Check pipette accuracy before and after grinding by dispensing 5 mL of diH20 onto a weigh boat on the balance If the weight is not within 10 of 5 g pipette needs to be adjusted Record weights on the bench sheet 3 Fill a 100 mL beaker with 90 acetone In subdued light in a vent hood place a sample filter in the 50 mL centrifuge tube and add 5 mL of 90 acetone solution using a pipette filter can be ripped into 4 pieces to make grinding easier Slide the centrifuge tube under the pestle and turn the knob to initiate grinding Grind using the mechanical grinder set up at a setting of 6 or less grinding the filter completely 4 Placing the centrifuge tube underneath to catch the runoff rinse the grinder and sides of centrifuge tube with another 5 mL of 90 acetone Turn the grinder on and allow the acetone to rinse the pestle If more than 5 mL is necessary for rinsing record total volume on the bench sheet 5 Place the tube into a rac
20. both are submerged 3 Press and observe the pH parameter display When the pH value has stabilized record the pre cal value and the temperature on the calibration worksheet then press If the calibration is successful Calibrated will appear at the top of the screen Record the cal value and the millivolt value on the calibration worksheet Millivolt values outside of the acceptable ranges may indicate a problem with the probe DH millivolt acceptable ranges buffer 7 gt 0 50 buffer 4 D180 50 buffer 10 gt 180 50 4 Press and the display will prompt you to enter a second pH standard value Remove the calibration cup rinse the probes with DI water dry the probes with a lab tissue and place the calibration cup with the second pH buffer solution onto the probes IV J Section 8 Page 3 of 5 Version 6 May 2014 Press and when the pH value has stabilized record the pre cal value and the temperature value then press If the calibration is successful Calibrated will appear at the top of the screen Record the cal value and the pH millivolt value and check acceptable ranges Repeat process for third pH buffer 1s necessary otherwise press Esc twice to return to the Calibrate menu so that you can begin the next calibration C Specific Conductivity Calibration Calibration solutions should be made with KCI potassium chloride A one point calibration is performed using a 1 430 micro siemens standard For spec
21. close lid Allow several seconds for the fluorometer to determine and set the range appropriate for the sample concentration Machine will beep until this is finished Press the button This will average the readings over 10 seconds Record the value when display says done If the fluorometer displays over the sample concentration is above the maximum level that the instrument can read 500 and must be diluted Put 9 ml of 90 acetone in a clean cent tube and pipette Iml of sample into tube Invert and read on fluorometer as above Raw fluorescence number should be multiplied by 10 Pour extract back into centrifuge tube and place in another rack Rinse cuvette with 100 acetone and drain between samples Use 3 cuvettes and alternate them to speed up analysis To clean centrifuge tubes pour excess extract into acetone waste receptacle Remove filter from tube and discard Rinse twice with tap water and once with DI water Place inverted in rack to dry Tubes should be acid washed occasionally to remove built up residue Computation of Chlorophyll a If the LRB fluorometer value is 1 0 or greater subtract this value from the fluorometer value before Chl a calculation For chlorophyll a concentration in ug liter parts per billion multiply the flourometer reading by this factor ml 90 acetone added ml of sample water filtered e g 10 75 0 133 or 10 50 0 200 Some of our projects have chla report sheets which will automatically cal
22. lost samples instruments out of calibration etc If conditions prevent sampling notify DWR coalition coordinator within one week e Representativeness The Lower Basin of the Cape Fear River is a diverse system composed of riverine and estuarine habitats Monitoring sites for this study were selected to adequately represent these regions The program uses sampling techniques developed for riverine and estuarine areas e Comparability All Lower Cape Fear River personnel follow the same protocols when collecting samples Standard Operating Procedures for sample collection are described in detail in Section 6 of this document This consistency in methodology ensures comparability of the data e Quality Control The use of laboratory blanks field duplicates lab control samples matrix spikes and duplicates and compound identification and quantification are components of the Quality Control methods used by the state certified contract lab The Quality Control procedures used by the contracted state certified laboratory are described for each method used to analyze samples A list of methods used by the state certified contract laboratory can be found in Appendix VIII e Precision Analytical precision is evaluated by analysis of laboratory quality control samples such as duplicate control samples matrix spike duplicates and sample duplicates Because the river is a flowing system duplicate field measurements do not sample the same volume of wat
23. overemphasized Required Chain of Custody forms and procedures should be employed Section 7 3 General Sample Collection Methodology e Refer to the appropriate field equipment checklist prior to each sampling trip e The preferred method of collecting a sample is to collect the water directly in the stream in the container used to transport the sample to the laboratory e During in stream sampling hold bottle at base and submerge into water neck down quickly turn the bottle until the mouth of the bottle 1s tilted up and facing into the current approximately 0 15 m below the surface If no current 1s present move the bottle forward in the upstream direction When using bottles that are pre loaded with preservative Le nutrient bottles with H2SO4 submerge the neck up bottle under the water quickly If sampling from a boat fill the bottles as to avoid collecting any compounds coming from the boat e In instances when a bucket must be used rinse the bucket thoroughly with water from the site prior to sample collection making sure to dump rinse water away from sampling area e When sampling from a bridge collect samples midstream on upstream side of the bridge e If using the Remote Bottle Filling Device RBFD Coli sampler or Long arm Figures 6 1 6 2 6 3 lower device to the surface and rapidly submerge so bottles fill below the surface e Be sure to leave ample air space in bottles to facilitate mixing by shaking Section 6 Page 2 of 7
24. should be analyzed in duplicate Fill bottle to about 95 full to allow for mixing before filtration samples should be placed on ice or refrigerated until filtration Filter samples as soon as possible maximum holding time is 24 hours One aliquot 1s filtered for each sample A bottle duplicate must be filtered once per ten samples During filtration the goal is to filter enough water to keep the chlorophyll a concentration below the UDL thereby eliminating an extract dilution step while allowing for enough to be analyzed accurately Shake the sample for 10 seconds to mix measure 20 mL in a graduated cylinder and pour into the filter funnel Apply suction not to exceed 6 in Hg 20kPa As the sample leaves the funnel turn off the suction to prevent possible cell rupture and chl a loss Look on the filter for a visible golden brown or green color If there is no color visible measure and filter another 20 mL Repeat until color is seen If the chlorophyll a concentration is expected to be less than 100 ug L one 50 mL aliquot may be filtered IV Section 9 Page 3 of 12 Version 6 May 2014 9 Fold filters in half with filtered material inside and place in aluminum foil wrapper Fold foil and label with a permanent marker including the station and volume filtered 10 Rinse graduated cylinder and filter funnels with di H2O 11 When all samples have been filtered filter 50 mL of di H20 as a laboratory reagent blank LRB 12
25. the revocation of this Agreement by DWQ and the return to individual upstream and downstream monitoring requirements as specified in individual NPDES permits of the PERMITTEES Stream sampling may be discontinued at such times as flow conditions in the receiving waters or extreme weather conditions will result in a substantial risk of injury or death to persons collecting samples Sampling may also be discontinued when environmental conditions such as a dry stream prevent sample collection In such cases for each day that sampling is discontinued DWQ Coalition Coordinator s shall be notified within one week of the discontinuance and written justification for the discontinuance shall be submitted with the monthly data submittal This provision shall be strictly construed and may not be utilized to avoid the requirements of this Agreement when performance of these requirements is attainable When there is a discontinuance pursuant to this provision sampling shall be resumed at the first opportunity after the risk period has ceased This Agreement may be modified by written consent of both parties DWQ or the PERMITEES may determine that it is necessary to request changes in monitoring frequency parameters or sites to be sampled Any such changes can only be made by a written amendment to this Agreement agreed to by DWQ and a majority of the PERMITTEES then parties to the Agreement The amendment shall be signed by PERMITTEES primary contact and b
26. 1t to charge 1 Print out WS 1 1 1 YSI Calibration Worksheet to record calibration metrics This sheet is to be stapled to the field data sheet for each day of sampling 2 Remove the sonde cap and inspect each probe Check the dissolved oxygen probe membrane for tears wrinkles or bubbles any of these will require membrane replacement DAB the membrane with a lab tissue to remove moisture III 3 4 gt Section 8 Page 2 of 5 Version 6 May 2014 Replace the cap leaving it loosely attached during calibration so that any barometric pressure changes can equilibrate inside the cap Make sure the sponge is moist Connect the cable to the sonde and power up the YSI 650 The unit will display the 650 Main Menu A dissolved oxygen probe reverse warm up check should be performed to ensure that the DO probe is functioning properly highlight Sonde run press amp and watch the DO saturation value The value should start high and decrease towards 100 If the number increases in value there may be a problem with the DO probe and the DO charge and gain need to be monitored when calibrating 6 Press Esc to exit to 650 Main Menu Calibration Highlight Sonde Menu and press amp enter button to enter Main sonde menu Enter Report and turn on the DO charge and pH mV functions Escape to Main sonde and enter Calibrate menu A Dissolved Oxygen Calibration 1 Dissolved Oxygen is calibrated using air saturation inside the sonde ca
27. 9 Page 10 of 12 Version 6 May 2014 YSI 5000 Calibration Log 3 M KCI pH 4 0 buffer solution pH 7 0 buffer solution Sample Collection and Preparation Collect samples in one liter plastic bottles Dirty bottles are cleaned by soaking in a 10 HCI bath for 24 hours then rinsing and drying It is always advisable to collect water directly in stream but a thoroughly rinsed bucket may be used Samples are kept on ice and in the dark until time of setup Samples should be run within 24 hours of collection Raise temperature of samples to 20 C by soaking in warm water measuring temperature with glass thermometer Store samples in the incubator until ready to set up Place glass BOD bottles in rack and record bottle numbers on BOD data sheet For each sample pour 750 mL of sample into a 1000 mL graduated cylinder and aerate for five seconds with aeration wand This allows the dissolved oxygen saturation to reach near 100 by either releasing oxygen from super saturated samples or adding oxygen to samples below saturation f air bubbles develop in the tops of the bottles during incubation it is likely because this step was not performed correctly Fill the 300 ml BOD bottles to the top with sample water and let bottles rest for 30 60 minutes Duplicates are run for each sample location A distilled water blank should be run in sequence at the beginning of the bottle rack and at the end A BOD of 20 2 in a blank indicates some interference
28. DL has been established using this data If a sample reads above 90 the UDL the sample must be diluted and re analyzed Section 9 Page 5 of 12 Version 6 May 2014 BS 1 2 1 Lower Cape Fear River Program Chlorophyll a Bench Sheet month year Station filter 1 filter 2 M18 NC11 90 acetone blank value LRB blank value mL filtered sample date Grinding time date Reading time date Room Temperature Sample Temperature Analyst s Signature Actual Chla solid stnd values Hi Lo Check Standard value must be within 10 of actual value if not the fluorometer may need calibration Pippettor check weight before Pippettor check weight after Section 9 Page 6 of 12 Version 6 May 2014 Aquatic Ecology Laboratory SOP 2 10 Water Column Cholorophyll a Determination Non Grinding Analysis of water column chlorophyll a using fluorometry Author s Matthew McIver Author s Matthew Mclver Reference s Turner Designs 1993 Model 10 AU 000 Laboratory Fluorometer User s Manual Part Number 10 AU 070 845 W Maude Avenue Sunnyvale CA 94086 Welschmeyer N A 1994 Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and phaeopigments Limnl Oceanogr 39 1985 1992 U S Environmental Protection Agency 1997 Methods for the Determination of Chemical Substances in Marine and Estuarine Environmental Matrices Second Edition EPA 600 R 97 072 U S Environmental Protectio
29. EMENT Agreement is made by and between the DIVISION OF WATER QUALITY NORTH CAROLINA DEPARTMENT OF ENVIRONMENT AND NATURAL RESOURCES or its successor agency or department DWQ and the NPDES Dischargers who have voluntarily approved this Agreement known and referenced collectively as the PERMITTEES The Agreement includes all the attached tables and appendices The purpose of this Agreement is to establish a formal agreement between DWQ and the PERMITTEES whose responsibilities include surface water monitoring and reporting within the Lower Cape Fear River Basin below Lock and Dam 1 to monitor strategically located surface water sites and parameters to obtain information on water quality in the basin PERMITTEES shall subcontract with one organization to collect instream monitoring data normally collected by the individual permittees Monitoring sites and parameters have been established by the PERMITTEES and DWQ such that the instream monitoring is more efficient effective flexible and basin oriented The PERMITTEES who are participating in this Agreement listed in Table 1 are exempted from any instream monitoring as specified in their individual NPDES permits beginning on the effective date of this Agreement and continuing for the duration of each permittee s participation in this agreement This Agreement does not affect any influent or effluent monitoring requirement or any other of the NPDES permit requirements of individual
30. Kenneth T Cowan Mayor J R Steigerwald Town Manager Tim Owens Town Manager Matthew W Jordan General Manager Page 5 of 17 Momentive Specialty Chemicals Elementis Chromium LP Town of Mount Olive Mt Olive WWTP Town of Burgaw Burgaw WWTP Town of Warsaw Warsaw WWTP Town of Carolina Beach Carolina Beach WWTP Cape Fear Public Utility Authority Northside WWTP LCFRP MOA NC0003395 NC0003875 NC0020575 NC0021113 NC0021903 NC0023256 NC0023965 Ronald Bazinet Site Leader Joel Barnhart Vice President Technical Charles Brown Town Manager Kenneth T Cowan Mayor J R Steigerwald Town Manager Tim Owens Town Manager Matthew W Jordan General Manager Page 5 of 17 Momentive Specialty Chemicals Elementis Chromium LP Town of Mount Olive Mt Olive WWTP Town of Burgaw Burgaw WWTP Town of Warsaw Warsaw WWTP Town of Carolina Beach Carolina Beach WWTP Cape Fear Public Utility Authority Northside WWTP LCFRP MOA NC0003395 NC0003875 NC0020575 NC0021113 NC0021903 NC0023256 NC0023965 April Hanson Environmental Engineer Joel Barnhart Vice President Technical Charles Brown Town Manager Kenneth T Cowan Mayor UR Steigerw Town Manager A ZB Pas Z A D E PA gt y i lt Tim Owens Town Manager Matthew W Jordan General Manager Page 5of17 Momentive Specialty Chemicals Elementis Chrom
31. P LCFRP MOA NC0003395 NC0003875 NC0020575 NC0021113 NC0021903 NC0023256 NC0023965 pritHansen Ra DAANET Joel Barnhart Vice President Technical Charles Brown Town Manager Kenneth T Cowan Mayor J R Steigerwald Town Manager Tim Owens Town Manager Matthew W Jordan General Manager Page 5 of 17 Momentive Specialty Chemicals Elementis Chromium LP Town of Mount Olive Mt Olive WWTP Town of Burgaw Burgaw WWTP Town of Warsaw Warsaw WWTP Town of Carolina Beach Carolina Beach WWTP Cape Fear Public Utility Authority Northside WWTP LCFRP MOA NC0003395 NC0003875 NC0020575 NC0021113 NC0021903 NC0023256 NC0023965 April Hanson Environmental Engineer Barnhart Vice President Technical Charles Brown Town Manager Kenneth T Cowan Mayor J R Steigerwald Town Manager Tim Owens Town Manager Matthew W Jordan General Manager Page 5 of 17 Momentive Specialty Chemicals Elementis Chromium LP Town of Mount Olive Mt Olive WWTP Town of Burgaw Burgaw WWTP Town of Warsaw Warsaw WWTP Town of Carolina Beach Carolina Beach WWTP Cape Fear Public Utility Authority Northside WWTP LCFRP MOA NC0003395 NC0003875 NC0020575 NC0021113 NC0021903 NC0023256 NC0023965 Hf Us Charles Brown April Hanson Environmental Engineer Joel Barnhart Vice President Technical wt Town Manager
32. QUALITY ASSURANCE QUALITY CONTROL MANUAL FOR THE LOWER CAPE FEAR RIVER PROGRAM Aquatic Ecology Laboratory University of North Carolina Wilmington Center for Marine Science 5600 Marvin K Moss Lane Wilmington North Carolina 28409 Version 6 October 2014 Table of Contents Section Number of Pages H Version o Changes md Updates u y ios 2 l era Ve EE EE I 2 Program Goals History and Mission Statement I OR RE e EE I d EE l 5 Station Descriptions and Sampling Schedule ccc cece cece 3 6 Standard Operating Procedures for Field Sampling 7 7 Chino EE eap Ee DL Cord a 3 8 Multi parameter Water Quality Meter Operanon 0 cece 5 9 Aquatic Ecology Laboratory SOPSsS 12 10 Data Management and Data Qualtlty 5 Lb iOUAJOC ie EE I 12 Reports and Publica ou gerne rs 4 Appendix I Memorandum of Agreement Appendix II LCFRP QA QC Subcommittee Appendix III LCFRP Advisory Board Appendix IV LCFRP Technical Committee Appendix V LCFRP Subscribers Appendix VI Curriculum Vitae of Scientists Appendix VII Aquatic Ecology Lab Certification Documents Appendix VIII Analysis Methods and SOPs Section 0 Page 1 of 2 Version 6 May 2014 section 0 Version 6 changes and updates L General Changes 1 Change Section Titles from center justified to left justified 2 Review date removed from headers 3 Sect
33. US dns Dunn runoff 35 1560 78 6401 8 miles dns Clinton WWTP 34 9186 78 3887 nonpoint impacts Just ups Great Coharie Ck 34 8347 78 3709 hog ops in watershed Just ups Black River hog 34 7933 783113 operations in watershed Ups of Black River 34 6402 78 3116 Hog operations in watershed 34 4641 78 2569 Great Coharie Creek at SR 1214 nr B8604000 Butler Crossroads Sampson Little Coharie Creek at SR 1207 nr Ingold Six Runs Creek at SR 1003 nr Ingold B8610001 Sampson B8740000 Sampson B8920000 South River at SR 1007 nr Kerr Sampson B8981000 Colly Creek at NC 53 at Colly st B9000000 Black River at NC 210 at Still Bluff p Pide UPS of Cape Fear 344312 78 1441 Pender Cape Fear River ups Indian Creek nr Dns DAK BASF and 34 3021 78 0137 Brunswick WIRO Phoenix Fortron Cape Fear River at Navassa dns of dns Progress Energy and RR bridge Leland Ind Pk 34 2594 77 9877 Brunswick WIRO Cape Fear River at S end of Horseshoe Bend nr Wilmington Ups NE Cape Fear River 34 2437 77 9698 Brunswick WIRO Dns Mt Olive WWTP DWO ambient stn 35 1784 77 9807 NE Cape Fear River at NC 403 nr Williams Sample from Bay Valley access Rd dns Bay Valley 35 1345 78 1363 WWTP Major trib to NE CFR Ag 35 028 77 8516 and Hog ops in watershed Dns Guilford Mills and Cogentrix WWTPs 34 9801 77 8622 ae Ups Wallace WWTP2 34 7168 77 9795 Wallace Little Rockfish Creek at NC 11 nr U
34. Version 6 May 2014 Place samples in coolers on ice for storage and transport to the lab 4 Parameter Specific Sampling Procedures A Chlorophyll a MOA required samples at stations M18 M61 NC11 DWR sampling protocol requires photic zone integrated sampling at stations where this sampling is feasible 1 e depth greater than meter which includes all three of the LCFRP sites Photic zone samples are collected using the lab line sampler Figure 6 4 The secchi depth is measured and multiplied times 2 to calculate the photic zone depth Lower the instrument to this depth pop the plugs and pull it slowly to the surface Station specific brown chl a bottles are filled directly from the lab line Rinse the lab line sampler with distilled water between stations Non MOA samples Chlorophyll a samples are collected in triplicate in 120 ml brown plastic bottles that are clean and dry Each bottle is filled separately about 0 25 m below the surface Secchi Depth Secchi Depth is collected as supplementary data not required by the MOA The secchi disc is mounted on an extending pole marked in tenths of meters Extend the pole and lower into the water on the shady side of the boat The last depth at which you can see the white versus black markings is recorded as the Secchi Depth Biochemical Oxygen Demand BOD samples are collected in 1 L plastic bottles that have been cleaned 24 hours in 10 HCI bath and dried Analysis must begin with
35. acts water quality management issues Education Ph D Estuarine ecology University of North Carolina at Chapel Hill 1992 M S Limnology and freshwater ecology University of Florida December 1978 Bachelor of Science Botany Ohio University June 1975 Professional Experience University of North Carolina at Wilmington Research Professor 2001 present Research Associate Professor 2000 2001 Research Scientist 1998 1999 Research Associate 1993 1998 Visiting Assistant Professor 1992 1993 College of Charleston Adjunct Professor of Environmental Studies appointed 2003 University of North Carolina Institute of Marine Sciences summer 1992 1994 Visiting Faculty Carolina Power and Light Co New Hill N C 1979 1987 Limnologist Professional Societies American Society for Limnology and Oceanography Estuarine Research Federation Member of ERF Governing Board 2003 2005 Southeastern Estuarine Research Society President 2004 2006 North American Lake Management Society Courses Taught Estuarine Biology Planktonology Limnology Ecology Biological Oceanography River Ecology Awards Elected Fellow American Association for the Advancement of Science 2007 UNCW Information Technology Innovation Award 2002 Aldo Leopold Environmental Leadership Fellow 2001 Ecological Society of America Large Community Comprehensive Planning Award 2000 N H County Tidal Creeks Program American Planning Association Peer Review
36. ae NC0026018 Kenneth Smith Mayor E GE 910 298 4647 PO Box 130 i Beulaville NC 28518 0130 D Town of Carolina Beach pcs Parisius NC0023256 Gil BuBois ge SE EE in s ern iia 910 458 2976 1121 N Lake Park Blvd arolina Beac rojects Director ill Raymond carollinabeach org Carolina Beach NC 28428 Town of Mount Olive e KE NC0020575 Charles Brown Town Manager i Tere Hinet 919 658 6538 PO Box 939 ount Olive wastewater ellsouth ne Mount Olive NC 28365 0939 Town of Warsaw E NC0021903 Shawn Condon Town Manager Myte Mays OR od E PO Box 464 WWTP Finance townofwarsawnc com 103 Warsaw NC 28398 e Momentive Specialty Chemicals E uda NC0003395 Ronald Bazinet Plant Manager e SOM AE 333 Neils Eddy Road Chemicals tom buller momentive com 5241 Riegelwood NC 28456 Fortron Industries LLC Fortron Industries LLC NC0082295 Gard Gershmel VP of Operations and Site Director ony Long 910 341 3152 Ro d M gary long ticona com PO Box 327 Wilmington NC 28402 Appendix VI Curriculum Vitae of LCFRP Scientists Michael A Mallin Present Position Research Professor Center for Marine Science University of North Carolina at Wilmington 5600 Marvin K Moss Lane Wilmington NC 28409 Telephone 910 962 2358 Email mallinm uncwil edu Website http www uncw edu cmsr aguaticecology laboratory Research Interests Ecology of phytoplankton and zooplankton urban and rural pollution sources nutrient and microbial pollution imp
37. alf filtered material on the inside and place in aluminum foil Fold foil and label with a permanent marker the station date and amount filtered Filters should be placed in a container with dessicant and kept in the freezer until analysis If analyzing nutrients from the filtrate return about 70 ml of filtrate to a rinsed sample bottle already labeled for analysis of dissolved inorganic nitrogen and or phosphorous These samples should be kept in the freezer Clean filter manifold by flushing with DI water A monthly 1 HCl rinse is recommended to further clean the apparatus Extraction of Chlorophyll a Remove foil wrapped filters from freezer In subdued light un wrap filters placing each filter in a 15 ml centrifuge tube with 10 ml of 90 acetone solution Place tube in a pre labeled rack The rack for chla tubes has pvc tubes cut to length to cover each tube to prevent light from entering the tube When the set is done or rack is full place rack in the refrigerator Steep for a minimum of 18 hours but no more than 24 hours Remember chlorophyll a is sensitive to heat and light so minimize each Fluorometric Analysis Turn on fluorometer and allow a 30 minute warm up Remove samples from refrigerator gently invert each tube several times and return to tube rack Place rack in the dark and allow to warm to room temperature Pour acetone from tube into the fluorometer cuvette about 1 2 full 1s fine Place tube in chamber and
38. ant into the fluorometer cuvette About one half full Is fine Place the tube in the chamber and close the lid Press the button This will range Section 9 Page 4 of 12 Version 6 May 2014 the sample and average the readings over 10 seconds Record the value when the display says done Record value to one decimal place without rounding Rinse the cuvette with 100 acetone and drain between samples Use 3 cuvettes and alternate them to speed up analysis Rinse the centrifuge tubes by pouring the excess acetone into the acetone waster container Use a squirt bottle full of DI water to blast the filter pellet out of the bottom of the tube and pour into the acetone waste container Place in a separate rack If the fluorometer value is gt 450 90 of the Upper Detection Limit 500 the sample must be diluted and re analyzed A Print out WS 1 2 1 and fill out appropriate information during dilution procedure B Place 9 mL of 90 acetone into a clean centrifuge tube using a clean pipettor tip Add 1 mL of the sample extract using another clean tip and invert several times to mix C Pour into a clean cuvette and read as usual D Be sure to add in Dilution Factor when calculating final Chlorophyll a concentration Clean the cuvettes after use by rinsing them 3 times with DI water Clean the centrifuge tubes by rinsing the tubes 3 times with DI water and invert in a rack to dry Tubes should be placed in the acid bath occasionally
39. b for re evaluation 1 Send data to NCDWQ for STORET entry 2 Print data for inclusion in LCFRP file 3 Enter data in Excel tables for Annual Report Section 10 Page 3 of 5 Version 6 May 2014 Lower Cape Fear River Program Data Status please initial and date each entry 2006 2007 EXCEL sheets to Data E mailed to Data presented to Month of Sampline Tri ita Entered on EXCEL LCFRP Subscribers Technical DWQ lt 90 days Collection Pane srt P EXCEL Doublecheck lt 45 days after after end of e committee collection collection month Cape Fear Estuary Cape Fear Riverine NECFR Watershed Black Ri November TI Watershed Cape Fear Estuary Cape Fear Riverine NECFR Watershed Black River Cape Fear Estuary Cape Fear Riverine NECFR Watershed Black River Cape Fear Estuary Cape Fear Riverine NECFR Watershed Black River Cape Fear Estuary Cape Fear Riverine NECFR Watershed Black River Cape Fear Estuary Cape Fear Riverine NECFR Watershed Black River Cape Fear Estuary Cape Fear Riverine NECFR Watershed Section 10 Page 4 of 5 Version 6 May 2014 Lower Cape Fear River Program Data Exclusion Sheet Month Station Data Excluded Reason for Data Exclusion Section 10 Page 5 of 5 Version 6 May 2014 Data Quality Objectives e Completeness It is imperative that all data is collected per schedule in the MOA Appendix D and analyzed to the levels dictated by the MOA Resampling may be necessary because of
40. by 4661 8 OLSZ WS ALIALLONGNOO snoanby oujawWOJON 4 ZL 9 0 Srr vd sualaWvsvd 031411430 e TIAHdOHO HO IINVIYONI NOJeq peisi siajaweJed ay jo jueujeJnseeui ay Jo papua qeueu si 0080 HZ OVON VSL Jo sjuawaumbas ay zaw Ajnp Dua Aioyesoqe paweu eAoqe ay 60y8Z ON uolBuluullAA eue sso unen 009S Su l5S euue N JO 19 u9O MONnD 487 bojo93 onenby Bunsr si j uieieqd Date 401329141199 103e 10q87 19jeMpunoJ49 19 eM9 SeM PUIOJEO YHON JuaWwuyseyy SS Jppy uueN qe1 Appendix VII continued sulIBBI A 1u y Y PPP gt K PLOZ TE ZT un Dien eq Heys ajeoynueo SIUL 8 9 ON 21021 442 e qejdaooe eq oj punoj pue peuiuiexe ueaq aney Aiojesoge ey jo Aouajoyoid pue sp10284 seunpeooud joujuoo Ajijenb 3uewidinbe Kbojopoujeui ey sejeorpur jnq pojeueueb ejep jo ypijea aajuesenB jou seop 9j821I492 SIU sjeoyyiieo siu Jo ed e epeui si 0080 HZ OVON WSL eoueejai Ag suoyejnbas jueurjeeaujeid pue 1ejempunolb sajem INS juenjje S3GdN Uim eoueiduio2 40J YMC 0 ejep Buuojiuoui podas pue jueuigoejjy uo pejsi Se sis jeue ejuawuonnus uuojied oj pereo qalay S 2243195 JUEN 104 491439 MINN q87 do o93 9nenby 0090 HZ DVON Pue 01 e es12 b1 L e SLZ EFL S D D N JO suorsi o4d au IM SDUEPIOIDE U NVYSONd NOILVOISILYAD ANHOJVHOSV I SI2YMOSIY YSLVM dO NOISIAIG S3OMhn OOS3M TVINLVN INV LNSWNOMSIANS FHL 20 LN3A LHVd3G VNITOHVO HLYON dO FLVLS Appendix VIII
41. c 82 tt6L vt oy ary xpe g sdn ent JU 001 YS 18 3 12 SUNY xig 0000pL34 d y t8 vt O 10001984 Y SLIBYOD WAN sdn ysng iu LOZI UWS J A991 suwyod 941 3 ler ve mh ee 8 f sjoeduir juroduou SpROISSOI omg MSD 9000 0 0 I 89 81 Ova uosdues 88 8 9816 lt 57 AAA mom sup sajru g au pLz T YS Ie seat auer paip O 9000 0 0 wosdues I0r9 8L 09S1 S Mount uunq sup 19d00 JU SI J out YINOS ow 0000Lv88 S VE even TOATY YORIG SZN SO00 0 0 yormsunig pesO 8L 8stE vt 000s9v88 CC vt NO 000v0988 z o Q WOJ jo sdn ysnf yeu yq Iy 19doopy Ju yeyu Je Ary Je94 Idep S d P I Bag ge uys ju rqurg ouroy Ju 3urpup NSTHA 2 000t0t0 quinto POLI SL SSSt vt OMA dI o oq opui PE SIN I JOALY 182 ode 0000S 8a l e ea IO YSU JUIY JU BAN E AA 000 0t0 auno 1107 82 t sup us juaiquie OMA dio UM Je 393912 uojsSutArT COAT 000lIvvT8H A A 5 mo db f eipeary 000t0t0 uope g SLIT 8L 696t r Dit weg pue 3907 jo sup jsnf SEq JU ON J IATA seo adep HON T pu MO SAPA DE Sp u pe SCH E Bole uey go eee SO00t0t0 t178I OY H 8v85 8L 9t19 vt J SOU JU 82N JE 9917 SUMOIG N 0S00rt8dH id SES SUSY Teuamamnspat svp DH uno pppp pp pppp pp 8juounuo uongls MONEHOSa dl A9QuIAN a PP wens gg pee apnjiduo opnigeT Ene D ous INOJ z o a J 00009t88 WS NO D H VN OI O2 ER OT ID S q V VH dd
42. cchi Disk On Boat ___ Chain of Custody Sheets Fill out Field Info Board __ Camera _ Post Float Plan at Boat Shop Field Gloves _ Hand towel _ Cellular Phone ___ Check for Boat Box and Keys _ Check engine position _ Call EChem 392 0223 Binoculars Boat Gas Card ___ FATES bag ___ Chla chain of custody Temperature Bottle Di water to rinse LabLine Section 6 Page 7 of 7 Version 6 May 2014 Typical Field Data Sheet LOWER CAPE FEAR RIVER PROGRAM Field Data Date Scientists Weather Tide Recorder Signature Depth Temp Spec Cond Turbidity Diss Ox DO Satur Station See cepi pH us Salinity psu NTU get o Light Att Section 7 Page 1 of 3 Version 6 May 2014 Section 7 Chain of Custody Chain of custody procedures are utilized in order to document the handling of samples from the time of collection through processing in the laboratory This process provides written documentation that can be used to trace the progress of the sample for quality assurance purposes Chain of Custody forms are customized for each trip for both Contract Lab COCs and AEL COCs see pages 2 and 3 for examples Sample Custody Procedures 1 Bottles are labeled with station name date preservative type and analysis parameter in the lab prior to the sampling trip 2 Bottles are filled at the water body at the previously selected stations Chain of Custody sheets will be completed reflecting the types and number of samples collected at ea
43. ch station as well as date time initial temperature and preservative COC sheets are pre populated and can be printed in the lab prior to each trip 3 Field investigators will keep the samples in their custody until they are delivered to the processing laboratory or relinquished at specified meeting point 4 A chain of custody form shall accompany all samples When possession of samples is transferred the individual receiving and the individual relinquishing delivering the samples will sign and date the form Section 7 Page 2 of 3 Version 6 May 2014 ENVIRONMENTAL CHEMISTS INC 6602 Windmill Way gt Wilmington NC 28405 1 Sample Collection and Chain of Custod Phone 910 392 0223 Fax 910 392 4424 Client UNCW CMS Lower Cape Fear River Program Estuary Boat Tri EMAIL ECHEMW aol com Collected By Report No le Type I Influe ud EE 7707 NN Sam SO e PRESERVATION Collection 2x Sample Identification E amp ANALYSIS REQUESTED ete ett tt NO NO 3 TKN Total P Ammonia o op per ect e A HB _ A EE EE TSS NO 2 NO 39 TKN Total P Ammonia EE E INI eg r TSS NO NO TKN Total P Ammonia Fecal NOTICE DECHLORINATION Samples for Ammonia TKN Phenol and Bacteria must be dechlorinated 0 2 ppm or less in the field at the time of collection See reverse side for instructions Relinquished By Date Time Received By Date Time ONE
44. ck Regional WWTP LCFRP MOA NC0023973 NC0026018 NC0039527 NC0075540 NC0086819 Matthew General Manager Kenneth Smith Mayor Jordan General Manager Carl Antos Chairman Marty Lawing County Manager Page 6 of 17 Cape Fear Public Utility Authority Southside WWTP Town of Beulaville Beulaville WWTP Cape Fear Public Utility Authority Walnut Hills Subdiv WWTP Brunswick Regional Water amp Sewer H2GO Belville WWTP Brunswick County NE Brunswick Regional WWTP LCFRP MOA NC0023973 NC0026018 NC0039527 NC0075540 NC0086819 Matthew W Jordan General Manager Kenneth Smith Mayor Matthew W Jordan General Manager Carl Antos Chairman Marty Lawing County Manager Page 6 of 17 Cape Fear Public Utility Authority Southside WWTP Town of Beulaville Beulaville WWTP Cape Fear Public Utility Authority Walnut Hills Subdiv WWTP Brunswick Regional Water amp Sewer H2GO Belville WWTP Brunswick County NE Brunswick Regional WWTP LCFRP MOA NC0023973 NC0026018 NC0039527 NC0075540 NC0086819 Matthew W Jordan General Manager Kenneth Smith Mayor Matthew W Jordan General Manager Carl Antos V4 Chairman m Marty Lawing County Manager Page 6 of 17 Cape Fear Public Utility Authority Southside WWTP Town of Beulaville Beulaville WWTP Cape Fear Public Utility Authority Walnut Hills Subdiv WWTP
45. culate chla concentration when the raw numbers are entered The mean and standard deviation for the replicate samples is also calculated and they are printed out in a form to be placed in respective data folders Section 9 Page 8 of 12 Version 6 May 2014 Lower Cape Fear River Program Chlorophyll a Analysis Non Grinding 1 UNCW Aquatic Ecology Laboratory month year Station replicate 1 replicate 2 replicate 3 Z lt SS EIS 5 Station replicate 1 replicate2 replicate 3 HO O a Q Lo O zz Station replicate 1 replicate 2 replicate 3 7 O ES Un s Qs 3s SC C Dropper Initials Time Date mL filtered Analyst s Initials Time Date 90 acetone blank value DI blank value Analyst s Signature Sample Temperature Dropper Initials Time Date mL filtered Analyst s Initials Time Date 90 acetone blank value DI blank value Analyst s Signature Sample Temperature Dropper Initials Time Date mL filtered Analyst s Initials Time Date 90 acetone blank value DI blank value Analyst s Signature Sample Temperature Section 9 Page 9 of 12 Version 6 May 2014 Aquatic Ecology Laboratory SOP 2 2 Biochemical Oxygen Demand Natural Surface Waters 5 and 20 day Author s Scott Ensign Matthew Mclver Author s Matthew Mclver Reference s Standard Methods 20 edition 5210 B L Introduction Purpose Bacteria
46. e just above Brunswick 34 3358 78 0534 03030005 confluence with Black R ml Black River below Lyons Thorofare UNCW AEL station 34 3513 78 0490 C Sw ORW 0303005 Ic B9030000 Cape Fear River ups maan Creek nr Downstream DF several point source Hose 34 3001 78 0137 CSw 0303005 Phoenix discharges NAV B9050025 Cape Fear River dns of RR bridge at Downstream oF several point source nne 34 2594 719877 SC 0303005 Navassa discharges B9050100 Cape Fear River at B end of Upstream of conmucnte with NE Brunswick 34 2437 71 9608 SC 0303005 Horseshoe Bend nr Wilmington Cape Fear River Basa VEER SE Near Belville discharge Brunswick 34 2214 77 9787 SC 03030005 nr Belville B9800000 Cape Fear River at Channel Marker Downstream of several point source Ree 34 1938 719573 03030005 61 at Wilmington discharges B9795000 Cape Fear a at Marker Downstream of several point source New Ha over 34 1393 71 946 03030005 discharges M35 B9850100 Cape Fear at PS Marker Upstream d Carolina Beach Bus de 34 0235 71937 SC 03030005 discharge B9910000 Cape Fear E at N Marker Downstream of Carolina Beach Suns 33 9456 71 9696 SA HQW 03030005 discharge SC Segoe a n a d e esed OSEE Car PvE 33 913 78 017 E 03030005 NCF6 B9670000 NE Cape Fear nr Wrightsboro pom E 343171 77 9538 NM 0303007 1 Collected by Land B8740000 SixRuns Creek at SR 1003 nr Ingold OP Steam sn 34 7933 78 3113 CSwORW 03030006 LCO B86 10001 Little Coharie Creek at SR 1207 nr Upstream G
47. e Great Coharie CA FOs Sampson 34 8347 78 3709 CSw 03030006 Ingold in watershed GCO B8604000 Great Coharie Creek at SR 1214 nr Downstream of Clinton CAFOs in Geesen 34 9186 78 3887 CSw 03030006 Butler Crossroads watershed BRN B8340050 E CAFOs in watershed Bladen 34 6136 78 5848 C 03030005 Elizabethtown Bs340200 Hammond che PHOTOS GATOS watershed 34 5685 78 5515 03030005 V Section 5 Page 2 of 3 Version 6 May 2014 Collected by Land e 1st bridge upstream of Cape Fear B210 B9000000 Black River at NC 210 at Still Bluff River Pender 34 4312 78 1441 C Sw ORW 03030006 V NE Cape Fear River at NC 403 nr Downstream of Mt Olive Pickle f NC403 B9090000 Duplin 35 1784 77 9807 CS 0303007 KE IA CAFOs in watershed wes sme zo cs TI mmm B9130000 Panther Branch Creek nr Faison Downstreamof Bay Valley Foods 35 1345 78 1363 0303007 Beer AAA A a CAFOs in watershed 35 0281 77 8516 0303007 nr Kornegay B9191500 NE Cape Fear River SR 1700nr Downstream Dr several point source Duplin 34 9801 77 8622 C Sw 0303007 Sarecta discharges Borgo Oe Bocki EE DWR Benthic station Duplin 34 7224 77 9814 CSw 0303007 Wallace B9490000 Angola Creek at NC 53 nr Maple Hill DWR Benthic station 34 6562 77 7351 0303007 South River at SR 1007 SR WC B8920000 Upst f Black Ri S 34 6402 78 3116 C Sw ORW 03030006 EEN Wildcat Ennis Bridge Road simpson zm mone csworws mmm NCF117 B9580000 NE Cape Fear River at US 117at DWR EE
48. ed Articles 6 of 74 total Mallin M A V L Johnson and S H Ensign 2009 Comparative impacts of stormwater runoffon water quality of an urban a suburban and a rural stream Environmental Monitoring and Assessment in press Appendix VI continued Mallin MA L B Cahoon B R Toothman D C Parsons M R McIver M L Ortwine and R N Harrington 2007 Impacts of a raw sewage spill on water and sediment quality In an urbanized estuary Marine Pollution Bulletin 54 81 88 Mallin M A V L Johnson S H Ensign and T A MacPherson 2006 Factors contributing to hypoxia in rivers lakes and streams Limnology and Oceanography 51 690 701 Mallin MA M R McIver H A Wells D C Parsons and V L Johnson 2005 Reversal of eutrophication following sewage treatment upgrades in the New River Estuary North Carolina Estuaries 28 750 760 Mallin MA M R McIver S H Ensign and L B Cahoon 2004 Photosynthetic and heterotrophic impacts of nutrient loading to blackwater streams Ecological Applications 14 823 838 Mallin M A K E Williams E C Esham and R P Lowe 2000 Effect of human development on bacteriological water quality in coastal watersheds Ecological Applications 10 1047 1056 Proceedings Technical Reports and other publications 5 of 93 total Mallin MA M I Haltom and B Song 2009 Assessing Fecal Bacteria Sources in the Wrightsville Beach N C Area Final Report CMS Report 09 04 Center for Marine Science Univer
49. er Duplicate field measurements do not always agree e Accuracy Analytical accuracy is evaluated by analysis of laboratory quality control samples surrogate standard matrix samples initial and continued calibration of equipment and blind samples For this project field accuracy is assessed using standard calibration techniques before and immediately following each sampling trip Section 11 Page 1 of 1 Version 6 May 2014 Section 11 OA OC Audits Annual internal audits of both field and laboratory portions of the Lower Cape Fear River Program will be conducted by members of the LCFRP The audits will consist of a critical review of Field data sheets Chain of custody sheets Laboratory log sheets Calibration worksheets Sample hold times Lab bench sheets Thermometer and reagent expiration dates Sample check in procedures There are no formal audit forms therefore the auditors should review SOPs Appendix VIII and have analysts perform a mock analysis During the mock analysis the auditor should review each step of the SOP and verify that all steps are performed and that all QA QC procedures are documented Appendix I Memorandum of Agreement Memorandum of Agreement Between The State of North Carolina s Division of Water Quality And The Lower Cape Fear River Program LCFRP Permittees Effective July 1 2011 through June 30 2016 This page was intentionally left blank MEMORANDUM OF AGREEMENT This AGRE
50. estrictions for sample preparation and analysis The value does not meet NPDES requirements Ql Holding time exceeded prior to receipt by lab Q2 Holding time exceeded following receipt by lab Not enough sample provided to prepare and or analyze a method required matrix spike MS and or duplicate MSD Indicates that the analyte was analyzed for but not detected above the reported practical quantitation limit PQL The number value reported with the U qualifier is equal to the laboratory s PQL Indicates the analyte was detected in both the sample and the associated method blank Note The value in the blank shall not be subtracted from the associated samples Sample not analyzed for this constituent This code is to be used if Xl Sample not screened for this compound X2 Sampled but analysis lost or not performed field error 3 Sampled but analysis lost or not performed lab error Elevated PQL due to insufficient sample size The presence or absence of the analyte cannot be verified The sample analysis results are not reported due to Zl Inability to analyze the sample Questions concerning data reliability PQL The Practical Quantitation Limit PQL is defined as the lowest level achievable among laboratories within specified limits during routine laboratory operation The Practical Quantitation Limit PQL is about three to five times the method detection limit MDL and rep
51. field data sheet Put the cap on the sonde allow to equilibrate for 15 minutes and record saturation value e Inthe event the MPWOM fails in the field scientists must return to the lab to get a new instrument or plan to resample on another day Figure 6 1 Long arm Sampling Pole This device holds bottles of all sizes and is used to extend out so that a bottle may be filled directly in stream when access to the water body is difficult Section 6 Page 4 of 7 Version 6 May 2014 Figure 6 2 Remote Bottle Filling Device RBFD This device is constructed of plastic rope and a rubber coated weight Its primary purpose is for in stream water sampling when access to the water is not possible i e from a bridge The bottles are placed on the platform and secured with the bottle hood caps extruding from the upper holes The hood bungees secure the hood The apparatus is lowered to the water surface and rapidly lowered so that the bottles fill below the surface Lowering Line Hood Hood Bungee Platform with Carrying Handle Plastic Coated Weight Section 6 Page 5 of 7 Version 6 May 2014 Figure 6 3 Remote Coliform Sampler This device is constructed of pvc rop and a weight Its primary purpose is for in stream water sampling when access to the water is not possible i e from a bridge and when the bottles do not fit in the RBFD Figure 7 2 The bottles are placed in the top and secured with a rubber band The apparatus i
52. g Summer includes the months of May June July August and September Twice monthly samples are to be collected at least ten days apart except when extenuating conditions arise Nutrient Sampling includes Ammonia as N NH3 Nitrate Nitrite as N NO2 NO3 Total Kjeldahl Nitrogen TKN and Total Phosphorus as P TP Metals Sampling EOM Every Other Month sample collection February April June August October and December for the following metals Aluminum Al Arsenic As Cadmium Cd Chromium Cr Copper Cu Iron Fe Lead Pb Mercury Hg Nickel Ni and Zinc Zn All analyses will be for total metals Metals monitoring was suspended per DWQ s April 2010 memorandum at the agreement of DWQ and the LCFRP LCFRP MOA Revision 2 February 26 2013 Appendix II QA QC Subcommittee Shawn O Connor Chair Global Nuclear Fuel Americas Phone 910 819 5869 PO Box 780 M C G 26 Fax 910 362 5869 Wilmington NC 28402 email Shawn Oconnor gnf com Matthew McIver LCFRP QA QC Supervisor UNCW CMS Phone 910 962 2357 5600 Marvin K Moss Lane Fax 910 962 2410 Wilmington NC 28409 email mciverm uncwil edu Todd Walton NC State Ports Authority Phone 910 251 5678 PO Box 9002 Fax 910 251 5886 Wilmington NC 28402 email Todd_Walton ncports com Emily Henderson International Paper Phone 910 362 4621 865 John L Riegel Rd Fax 910 334 3702 Riegelwood NC 28456 email Emily henderson ipaper com Appendix III
53. he bottom Open tab at side top of pH probe Immerse probe tip in 7 00 pH buffer and when S is constantly displayed in the upper left of the display press Standardize Rinse probe with distilled water and dab with a kimwipe Immerse probe tip In 4 00 buffer and when S 1s displayed press Standardize display will show a diagnostic and state if the probe is good then switch back to measurement mode VI VII Pa SUMI Section 9 Page 11 of 12 Version 6 May 2014 Wash and dab the probe and place in each BOD recording sample pH on data sheet To store probe close the tab and place in 3M KCl Do not use pH buffer Initial DO Measurement To measure DO in each bottle place the BOD probe into the bottle and turn on the stirrer Any air bubbles adhering to the sides of the bottle and or the probe will spin up towards the surface Raise probe slightly to allow these bubbles to escape Allow 30 seconds for DO value to equilibrate If the initial DO is gt 9 0 the sample is supersaturated and air bubbles will form in the BOD bottles over the 5 day incubation period Return sample to Grad Cylinder and swoosh Record DO value on the bench sheet value is recorded to the tenth place Rinse and dab the probe between each station Each bottle should be checked for air bubbles on the sides of the bottle Use a plastic stopper to tap on the bottle and force the bubbles to rise to the top Stopper and cap each bottle Ex
54. hemical Oxygen Demand Procedure North Carolina Department of Environment Health and Natural Resources Division of Environmental Management Raleigh N C NCDEHNR Five Day BOD Test Procedure Bottle Method Technique North Carolina Department of Environment Health and Natural Resources Division of Environmental Management Raleigh N C Section 9 Page 12 of 12 Version 6 May 2014 Typical BOD Bench Sheet BOD Bench Sheet Lower Cape Fear River Program River Boat Trip Collection Set Up 5 Day Reading 20 Day Reading Scientist Analyst Analyst Analyst Day Day Day Day Date Date Date Date Time Time Time Time Incubator Temp Incubator Temp Incubator Temp Bottle Field pH RE 5 day pH 5 day DO stanc ME W mas mo MEME Lo d Section 10 Page 1 of 5 Version 6 May 2014 Section 10 Data Management and Data Ouality An integral part of any research program is the proper handling of data This section describes data flow and quality control procedures used by the AEL with regards to Lower Cape Fear River Program data Data Management LCFRP data from the state certified contract laboratory 1s sent to the Program Research Director When the Program Research Director accepts the data as valid data required per the Memorandum of Agreement is entered into Microsoft Excel spreadsheets The Excel spreadsheet is checked for data entry errors and the final copy 1s reviewed by the Pr
55. hone numbers Identify the facility name and permit number Provide a list of members that are no longer active in the LCFRP Provide a list of changes in members names ownerships and discharge locations Summarize all quality assurance and quality control issues and any field audits conducted Summarize any significant issues special studies or projects Describe any required data collection that was missed and provide an explanation Review the monitoring program and suggest potential MOA modifications Provide the Coalition s Website Address LCFRP MOA Page 15 of 17 Table C 1 File Format For Coalition Data Reporting Su d d Mui N SON TON 1 54 N CON ZON quii N NJL 1 84 N NM 1 34 N CH D D D yu WAudoiomt dall od Jed 10 625 665 Seat eto J je lod fost x E E oc c quia INPISIY uui onpisoy p pu dsns r rus NEN dei co I Vin Amm NS eet ES EEE a umaman ma E um ERRRREERE Ll pu e a NR E O O O O O O au E dei dei dei dei e e EE i kent CN vi em ee pa v gemet SES CH CH N N N N N S o 2 a a g A A a Ci CON O O S O aA zi a a Aa i N ol c c ei ol c ES en e e en c en 6 owl Al al v s No O No No e NS bk en rm p rm dei N 00 00 GC GC en N N ri ON D ON ON en Y lt ea ca ca ca Q Q Q The reporting format table continues with metals a
56. ield the protocol 1s to return to collect data on another day X pM A y Aquatic Ecology Laboratory SOP 1 1 YSI Multi parameter Water Quality Sonde Operation for NC DWQ Field Certification Compliance Development date October 2007 Author s Scott Ensign Matthew Mclver Revised October 2014 Revision 4 Author s Matthew Mclver Reference s YSI 6820 6920 Sonde 650 MDS operation manuals NC DWR Lab Certification Webpage http portal ncdenr org web wdq lab cert I Introduction Purpose The quality and accuracy of data obtained using a multi parameter water quality sonde is dictated by several factors including the proper functioning of the individual probes the calibration procedure and the use of the instrument when taking measurements This protocol is designed to comply with the rules of the North Carolina Division of Water Resource s field lab certification program and must be performed each day measurements are made Using this protocol ensures not only quality and accurate data but also documentation of the procedures used to obtain this data This protocol 1s specifically indicated for the use of the YSI 6820 6920 sonde with a 650 MDS display II Start Up The YSI 650 display unit batteries automatically discharge over time therefore charging for 12 24 hours prior to sampling Is recommended The unit should not be left charging for periods of more than 24 hrs Be sure to power off the YSI 650 before leaving
57. ific situations where the measured value will be within a small range you may select a standard that more closely matches that range I 2 A 9 10 From the Calibrate menu choose Conductivity from the Conductivity menu choose SpCond Enter the standard value in milli Siemens cm Fill the conductivity calibration tube with 12 mls of used calibration solution and slide the tube onto the Conductivity probe to rinse Dump the used solution refill with fresh solution and insert onto probe Ensure that the round hole on the conductivity probe is covered with calibration solution Press and watch the display for temperature and conductivity values to equilibrate When the reading is stable record the pre cal value and temperature then press gt If the calibration is successful Calibrated will appear at the top of the screen Record the cal value Press Esc thrice to return to the Main menu and scroll down to highlight the advanced menu press gt and select cal constants Record the conductivity cal constant which should be 4 5 to 5 5 1f not the probe may be bad Esc once select report and turn off the DO charge and pH millivolt readings Remove the calibration tube pour used solution into the used container and rinse both the tube and the probe with distilled water Press Esc twice to return to the calibrate menu D Turbidity Calibration A one point calibration is performed using a 0 NTU standard distilled water
58. in 24 hours of collection Fecal Coliform Bacteria Enterococcus Fecal coliform samples are collected in sterilized plastic 120 mL bottles from the contract lab Sample must be taken directly in stream using Coli sampler when unable to reach water Analysis must begin within 8 hrs of collection Metals Metals Sampling has been suspended by NC DWR till further notice Metals samples are collected in a liter plastic bottle containing nitric acid Samples are stored on ice during transport to the laboratory Mercury samples may be held for no more than 28 days Samples analyzed for metals other than mercury may be held for no more than six months from date of collection Samples must be taken directly in stream using the RBFD when necessary Total Suspended Solids Aliquots of water for total suspended solids measurements are removed from non preserved sample bottles provided by the contract lab Every attempt is made to analyze samples within 24 hours but in no case are samples held more than 7 days Nutrients Nitrate Nitrite Total Kjeldahl Nitrogen Ammonium Total Phos All nutrients are analyzed from the same sample collected in plastic liter bottles containing sufficient HoSO4 to preserve sample at a pH from 1 5 to 2 Bottles are provided by and acidified by the contract lab Nitrate samples will be processed within 48 hours of sampling TKN ammonium and total phosphorus samples will be processed within 28 days of collection Nutrient
59. ing equipment Delete sheets describing backup instrument use Page 6 Update YSI calibration worksheet Section 9 Page 1 Update introduction paragraph Pages 2 3 4 5 Update Chlorophyll Grinding SOP Page 6 Update Chlorophyll Grinding Bench Sheet Page 9 10 Update Chorophyll Non Grinding SOP Section 10 Page 1 Change principal investigators to Research Director Page 1 Change NC DWQ to NC DWR several places Section 0 Page 2 of 2 Version 6 May 2014 Table 11 1 Delete Table can be found in Appendix V Section 11 Page 1 Change technical committee members These members will also be on the QA QC subcommittee of the LCFRP to members from the QA QC subcommittee of the LCFRP Page 1 Add information regarding the use of SOP s and QA QC manual to develop audit guidelines Delete all pages containing audit forms pages 2 32 Section 12 Update Publication List Appendix I Delete actual SOPs and insert table with laboratory method codes Move to Appendix VIII Appendix I is now MOA Appendix II Split lists into separate Appendices Appendix II is now QAQC Subcommittee list Appendix III Move Scientist CVs to Appendix VI Appendix III is Advisory Board List Appendix IV Benthic Ecology Lab info deleted Appendix IV 1s Technical Committee list Appendix V Move MOA to Appendix I Appendix V is Subscriber list Appendix VI Move Lab Certification
60. iology University of North Carolina Wilmington Wilmington NC May 1997 Bachelor of Science Microbiology minor in Genetics North Carolina State University Raleigh NC December 1991 Professional Experience Lecturer and Educator Have given over 35 presentations regarding water quality research and science careers Board Of Directors Cape Fear River Watch June 2000 August 2002 Official Biologist June 1999 Present Shallotte Point Volunteer Fire Department Annual Flounder Fishing Tournament Shallotte Point North Carolina Official Biologist June 2000 Greater Wilmington King Mackerel Tournament Wilmington North Carolina Boat Captain for film crew August 1998 Captained 28 foot vessel for filming of University of North Carolina at Wilmington Documentary Currents of Hope Reclaiming the Neuse River Lower Cape Fear River Program Member of Technical Education and QA QC committees June 1995 Present Graduate Research Assistant July 1994 May 1995 University of North Carolina at Wilmington Department of Biological Sciences Water quality analyses including water chemistry physical parameters using multi parameter water quality probe fecal coliform bacteriology and field sampling Performed nutrient limitation bioassays phytoplankton identification data compilation and supervised an undergraduate assistant for the New Hanover County Tidal Creeks Project and The Cape Fear River Project Appendix VI continued Ma
61. ion LCFRP MOA Page 11 of 17 Data Qualification Codes When reporting data the DWQ s data qualifier codes must be used to provide additional information regarding data quality and interpretation The current set codes are subject to change of qualifier codes to be used is provided in Table B 2 Review the data qualifier codes at least once a year and utilize the most current set being utilized by the DWQ laboratory Use the following website to check for changes in the qualifier codes http portal ncdenr org web wq lab qualityassurance Table B 2 Data Qualification Codes For Use With Coalition Data current as of April 20 2011 Code Definition Value reported is the mean average of two or more determinations This code is to be used if the results of two or more discrete and separate samples are averaged These samples shall have been processed and analyzed independently e g field duplicates different dilutions of the same sample This code is not required for BOD or coliform reporting since averaging multiple dilutions for these parameters is fundamental to those methods Results based upon colony counts outside the acceptable range and should be used with caution This code applies to microbiological tests and specifically to membrane filter MF colony counts It is to be used if less than 10096 sample was analyzed and the colony count is generated from a plate in which the number of colonies exceeds the ideal ranges i
62. ion 4 was deleted and information was added to Section 1 QA QC subcommittee list is now Appendix II Each Section beginning with 5 changes by one number 1 e Section 6 is now Section 5 4 Combine Section 11 and 12 now is Section 10 II Section Changes Section Page Add information paragraph from Page 1 Section 4 which was deleted Section 2 Section 3 Section4 Section 5 Section 6 Section 7 Section 8 No Changes Page 1 Add text description of organization Delete Page 2 organizational chart Page 1 Paragraph 2 change maintained as part of this document to maintained in the UNCW AEL Page I Paragraph 2 change final sentence to Below is a list of personnel working in the UNCW AEL at the time of this revision Page 1 Add signature sheet scan with signatures Table 5 1 Delete stations SPD M42 BCRR BC117 add SRWC Figure 5 1 Delete stations SPD M42 BCRR BC117 add SRWC Delete Figure 6 1 refer to table in Memorandum of Agreement Appendix I Page I Add Safety section Page 2 Add protocol for measuring Secchi Depth Pages 7 16 Update field equipment check lists and field data sheets No Changes Page I Change Section title from Equipment Maintenance and Calibration to Multi Parameter Water Quality Meter Operation Page 1 Introduction Paragraph Delete use of backup equipment add procedure for malfunction
63. ischargers 2 Program History In 1989 90 the Environmental Management Commission announced its intention to designate the Lower Cape Fear River as High Quality Waters The basis of this designation was the presence of Primary Nursery Areas in the Cape Fear River Estuary Local industries municipalities and economic development interests were very concerned that such action would adversely impact future economic development in the area A delay in classification was allowed based on the offer by concerned parties to obtain data and provide alternate management strategies The local Committee of 100 took the lead and formed the Lower Cape Fear River Water Quality Study Committee which included representatives from industry local government environmental management conservation economic development and the University The Lower Cape Fear Committee hired a consulting firm to gather existing data and to produce a review and analysis of the current status of the river Based on the report the Lower Cape Fear Committee developed short and long term management plans which were submitted to the NCDWOQ for review by the Environmental Management Commission The long term plan included establishment of the Cape Fear River Program to be funded by the users and the general public To provide objectivity the program was to be operated by UNCW In the spring of 1993 the NCDWO rejected the plan but encouraged the establishment of the Cape Fear River Program
64. ium LP Town of Mount Olive Mt Olive WWTP Town of Burgaw Burgaw WWTP Town of Warsaw Warsaw WWTP Town of Carolina Beach Carolina Beach WWTP Cape Fear Public Utility Authority Northside WWTP LCFRP MOA NC0003395 NC0003875 NC0020575 NC0021113 NC0021903 NC0023256 NC0023965 April Hanson Environmental Engineer Joel Barnhart Vice President Technical Charles Brown Town Manager Kenneth T Cowan Mayor J R Steigerwald Town Manager lt Ti ens Town Manager Matthew W Jordan General Manager Page 5 of 17 Momentive Specialty Chemicals NC0003395 April Hanson Environmental Engineer Elementis Chromium LP NC0003875 Joel Barnhart Vice President Technical Town of Mount Olive NC0020575 Mt Olive WWTP Charles Brown Town Manager Town of Burgaw NC0021113 Burgaw WWTP Kenneth T Cowan Mayor Town of Warsaw NC0021903 Warsaw WWTP J R Steigerwald Town Manager Town of Carolina Beach NC0023256 Carolina Beach WWTP Tim Owens Town Manager Cape Fear Public Utility Authority NC0023965 X LL Northside WWTP Matthew W Jordan General Manager ee LCFRP MOA Page 5 of 17 4 E PE a S Pg j e ES P S 3 E P d F P z Z A Ce Cape Fear Public Utility Authority Southside WWTP Town of Beulaville Beulaville WWTP Cape Fear Public Utility Authority Walnut Hills Subdiv WWTP Brunswick Regional Water amp Sewer H2GO Belville WWTP Brunswick County NE Brunswi
65. j ak j td ao dT amp uonboug Suir dureS pue s 1939 018 18 q suongejs Sundues JAAT penuguo J V 9Iqe L APPENDIX B SAMPLE COLLECTION AND ANALYSIS Sample Collection Procedures Sample collection shall be performed by trained personnel with NC DWQ certified laboratories in accordance with the DWQ NPDES Discharge Monitoring Coalition Program Field Monitoring Guidance Document May 2008 and subsequent documents Alternate collection procedures may be considered if reported to and approved by the DWQ Coalition Coordinator s prior to use Any approved alternate sampling procedures will be documented in writing by the LCFRP Laboratory Analysis All laboratory analyses shall be performed at a DWQ certified laboratory using approved methods as prescribed by 40 CFR part 136 or other methods certified by the DWQ Laboratory Certification Branch http portal ncdenr ore web wq lab cert or the Director of DWQ Section 40 of the Code of Federal Regulations part 136 40CFR136 can be accessed on the web at the following address http portal ncdenr org web wg lab cert nonfield rules Reporting levels will be at least as stringent as the target reporting levels used by the DWQ Laboratory For guidance purposes Table B 1 lists target reporting levels for each parameter based on the reporting levels of the DWQ Laboratory The lowest possible analytical limits for all the parameters should be pursued Table B 1 DWQ Laboratory Reporting Limits
66. k covered with tape A masking tape label is placed adjacent to each row of tubes to indicate which samples are in that row When the rack is full place it ina refrigerator at 4 C Use 100 acetone to rinse the pestle catch the used acetone into a waste container 7 Steep samples for a minimum of 2 hours but no more than 24 hours DWO asked us to remain consistent with steep times therefore we attempt to read chla the same day they are ground Longer steep times should be noted on the bench sheet Fluorometric Analysis Turn on the fluorometer at least 24 hours before reading samples to allow for warm up The fluorometer must be calibrated bi monthly Check the calibration after warm up with the solid secondary standard The fluorometer value must be within 10 of the actual value see LS 1 7 1 or calibration is required SOP 1 7 If calibration is not necessary record the solid secondary standard values on the bench sheets 1 Remove tube racks from the refrigerator cover the tops of the tubes with a clipboard and shake vigorously Centrifuge each tube at 1000 g for 5 minutes Measure the temperature of the tubes with the infrared thermometer If temperature is not approximately 70 F then let samples sit for a few minutes to warm up to room temperature 2 A Secondary Check Standard should be read at the beginning and end of each sample set See AEL SOP 1 7 to make and analyze the standard 3 To analyze a sample pour the supernat
67. l of at least 1 0 mg L G4 Evidence of toxicity was present This is generally characterized by a significant increase in the BOD value as the sample concentration decreases The reported value is calculated from the highest dilution representing the maximum loading potential and should be considered an estimated value G5 The glucose glutamic acid standard exceeded the range of 198 30 5 mg L G6 The calculated seed correction exceeded the range of 0 6 to 1 0 mg L G7 Less than 1 mg L DO remained for all dilutions set The reported value is an estimated greater than value and is calculated for the dilution using the least amount of sample G8 Oxygen usage is less than 2 mg L for all dilutions set The reported value is an estimated less than value and is calculated for the dilution using the most amount of sample The DO depletion of the dilution water blank produced a negative value J Estimated value value may not be accurate This code is to be used in the following instances dk Surrogate recovery limits have been exceeded J2 The reported value failed to meet the established quality control criteria for either precision or accuracy J3 The sample matrix interfered with the ability to make any accurate determination J4 The data is questionable because of improper laboratory or field protocols e g composite sample was collected instead of grab plastic instead of glass container J5 Temperature limits exceeded samples frozen or
68. l respiration requires oxygen and in natural surface waters it is beneficial to quantify this oxygen demand Organic material introduced to surface waters can often be problematic in that the resultant increase in oxygen demand may lead to oxygen depletion a stressful event for aquatic organisms There are also several chemical processes in surface waters that will result in oxygen demand thus the name Biochemical Oxygen Demand or BOD Summary of Method To quantify BOD water 1s collected from location of interest and transported to the lab The water is aerated such that saturation 1s achieved The water is then placed in special BOD bottles and the dissolved oxygen measured The bottles are placed in an incubator with constant temperature 20 C for a set number of days and then the dissolved oxygen is measured again The amount of oxygen used is calculated through subtraction and this number is the BOD value A standard of 5 or 20 days 1s typically used II Supplies Instruments One liter plastic bottles 300 ml glass BOD bottles stoppers and caps Coated metal BOD bottle racks 1 liter graduated cylinder plastic wand with perforated Plexiglas disk see attached photo incubator set at 20 Celsius 1 Dissolved oxygen meter with BOD probe Glass thermometer pH meter Contrad 70 laboratory soap mixed 2 v v 2ml per 100 ml of DIH20 Bleach DI H20 BOD bench sheet Kimwipes Barometer III IV 00 NO Section
69. land NC 28451 Calvin Overcash Elementis Chromium LP Elementis Chromium LP NC0003875 TBA Site Manager 910 675 7229 5408 Holly Shelter Rd calvin overcash O elementis com Castle Hayne NC 28429 Shawn O Connor Global Nuclear Fuels Americas Global Nuclear Fuels NC0001228 Shawn O Connor Environmental Specialist 910 819 5869 PO Box 780 M C G 26 Shawn Oconnor gnf com xl Wilmington NC 28402 Manager Environment Health Ed Kreul E International Paper NC0003298 Ed Kreul amp EN _ EE EE 910 362 4883 John L Riegel Road y y paper Riegelwood NC 28456 Invista S aR L i Environmental Health and Safety Rick Bayless 4600 Highway 421 North Invista S aR L NC0001112 Rick Bayless Manager E EE 910 341 5912 PO Box 327 Wilmington NC 28402 NE Brunswick Regional WWTP EE eegene Jern W Bierce Director of Public Utilities pans anon 910 755 7921 PO Box 249 WWTP bblanton brunsco net i Bolivia NC 28422 Sie CFPUA Ge Pear Public CUM NC0039527 Matthew W Jordan CFPUA General Manager B 910 332 6646 235 Government Center Drive Walnut Hills S D WWTP beth eckert cfpua org Se Wilmington NC 28403 Brunswick Regional Water Scott Hook and Russ Scott Lewis 910 371 9949 ex Ve E amp Sewer H2GO Nee Lane EE slewis h2goonline com 103 EE S i Leland NC 28451 Duke Energy Progress ee NC0001422 Allen Clare Plant Manager n Nar ino uM ma 910 343 3244 801 Sutton Steam Plant Rd Te Berrie Wilmington NC 28401 i Town of Beulaville rus SC
70. lina Estuaries 28 750 760 Mallin MA M R Mclver S H Ensign and L B Cahoon 2004 Photosynthetic and heterotrophic impacts of nutrient loading to blackwater streams Ecological Applications 14 823 838 Mallin MA L B Cahoon M R McIver and S H Ensign 2002 Seeking science based nutrient standards for coastal blackwater stream systems Report No 341 Water Resources Research Institute of the University of North Carolina Raleigh N C Mallin MA M H Posey M R McIver D C Parsons S H Ensign and T D Alphin 2002 Impacts and recovery from multiple hurricanes in a Piedmont Coastal Plain river system BioScience 52 999 1010 Mallin M A S H Ensign MR McIver G C Shank and P K Fowler 2001 Demographic landscape and meteorological factors controlling the microbial pollution of coastal waters Hydrobiologia Appendix VII Aquatic Ecology Lab Certification AFA LVL NCDENR North Carolina Department of Environment and Natural Resource Division of Water Resources Water Quality Programs Pat McCrory Thomas A Reeder John E Skvarla IIl Governor Director Secretary MEMORANDUM DATE January 1 2014 Dre Certified Laboratories FROM Kent Wiggins Division of Water Resources Laboratory Section Chief SUBJECT 2014 North Carolina Wastewater Groundwater Laboratory Certification Renewal The Department of Environment and Natural Resources in accordance with the provisions of NC GS 143 215 3 a 10 15
71. ll other Memorandum of Agreement required analyses are performed by a state certified analytical laboratory SOPs and laboratory data sheets for the AEL performed analyses are provided here Contract laboratory methods are included as Appendix VIII of this document Aquatic Ecology Laboratory SOP 1 2 Fluorometric Analysis of Chlorophyll a Using Grinding Development date 2002 Author s Matthew Mclver Revised April 2014 Revision 3 Author s Matthew Mclver Reference s EPA method 445 0 Turner Designs 10 AU User s Manual Welschmeyer N A 1994 Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and phaeopigments Limnl Oceanogr 39 1985 1992 L Introduction A Purpose Chlorophyll a is analyzed in order to quantify phytoplankton biomass This method is indicated for samples that require analysis using NC DWO certified methods B Summary of Method A selected volume of water 1s filtered through glass fiber filters concentrating the phytoplankton The filters are frozen immersed in acetone and then macerated with a tissue grinder Freezing and grinding ruptures the cells releasing the photosynthetic pigment chlorophyll a which dissolves in the acetone The chlorophyll a in the acetone is quantified using a fluorometer with a specific set of filters and light source which eliminates the need for acidification to address spectral interference from pheophytin and chl b This method require
72. ls 2 3 Fill the bottom portion of the calibration cup with distilled water Screw the calibration cup to the YSI sonde body From the Calibrate menu choose Turbidity then choose 1 point Enter 0 for the standard value and press gt Observe the turbidity value and select clean optics at the top of the screen to wipe the probe When the value stabilizes record the pre cal value then press gt If the calibration is successful Calibrated will appear at the top of the screen Record the cal value Press Esc twice to return to the Calibrate menu Remove the calibration cup and discard solution Post Sampling Calibration Check Lab certification rules dictate that after data collection the multi parameter water quality sonde should be checked to make sure the calibration has not drifted out of acceptable ranges A Dissolved Oxygen VI Section 8 Page 4 of 5 Version 6 May 2014 The dissolved oxygen calibration is checked at 100 air saturation Dab the membrane with a lab tissue and screw storage cap on loosely After a 15 minute equilibration period record the appropriate data on the worksheet The DO percent saturation acceptance range 1s 95 to 105 B pH The pH calibration should be checked with two buffers that bracket the values read in the field The acceptable range is within 0 1 standard units C Specific Conductivity The specific conductivity calibration is checked with two buffers which bracket the values read in
73. n Agency Cincinnati Ohio I Introduction Chlorophyll a is analyzed as a quantification of phytoplankton biomass This method employs the use of a specific set of filters which minimizes the interference from chl b and phaeopigments while removing errors inherent in the acidification procedure II Supplies Instruments Light proof 120mL brown Nalgene sample bottles Di H20 90 acetone 15 mL screw cap plastic polypropylene centrifuge tubes with racks Forceps Aluminum foil 3 funnel filtration manifold 25 mm glass fiber filters nominal pore size of 0 7 micron III Sample Collection and Preparation Samples should be collected in light proof bottles in triplicate and stored on ice or refrigerated until filtered 2 Filter samples as soon as possible maximum holding time is 24 hours 3 Using the 3 funnel manifold simultaneously filter 50 ml volume may vary depending on turbidity and algal concentration from each bottle 4 Invert sample several times to mix measure volume in a graduated cylinder and pour into filter funnel IV VI SE NO Section 9 Page 7 of 12 Version 6 May 2014 Apply vacuum pressure should not exceed 6 in Hg 20kPa Turn off the vacuum as the sample leaves the funnel allowing vacuum on a dry filter may result in chlorophyll loss A laboratory reagent blank LRB is made by filtering 50 ml of distilled water This blank filter 1s treated like the sample filters Fold filters in h
74. nd comment columns on the next page Page 16 of 17 LCFRP MOA Table C 1 Continued File Format For Coalition Data Submittals MULI 122032049 JU yur 8H A1n249 A N c fii 3H Ana lA un Sy DIUISIY sss CIA EEE yu y umnurun y wem Epp Le A vmm awa CH EE yu qd peog T e EIS II D EE EE ME MINER UN RUN wem ETT III sas ET o EMB AAA Sa qa 1293030419 Comments 2 5 of rain on 8 31 2002 Nutrient Sample Spilled Secchi depth 1 2 meters Page 17 of 17 LCFRP MOA APPENDIX A LCFRP MONITORING PLAN Table A 1 LCFRP Sampling Stations Parameters and Sampling Frequency Revised Latitude Longitude Station Comments dd dddd dd dddd hog farm area 34 6136 78 5848 just dns of Lock and Dam 1 34 3969 78 2675 DWO ambient stn dns 34 3353 78 2011 Station Number B8340050 County Bladen Location Description Browns Creek at NC87 nr Elizabethtown Hammond Creek at SR 1704 nr Mt Olive Cape Fear River at NC 11 nr East Arcadia Livingston Creek at Wright Corp Walkway nr Acme Wright Corp Cape Fear River at Neils Eddy C URN 34 3555 78 1794 Columbus Landing nr Acme ambient stn Cape Fear River at Intake nr Hooper Wi DAK intake just ups of 343358 78 0534 Brunswick WIRO i Black River B8340200 Bladen B8360000 Bladen B8441000 Columbus B8450000 B8465000 T 13 nr Cooper Sampson B8470000 South River at
75. nd samples are analyzed in the AEL UNCW using non certified procedures In situ readings of temperature pH conductivity dissolved oxygen turbidity and salinity are measured using a YSI Multi Parameter Water Quality Meter MPWQM at the same location as the bottle samples are collected The AEL UNCW is NC DWR certified for field parameters and chlorophyll a analysis Appendix VI Standard Operating Procedures SOPs are reviewed and revised periodically as needed and the revision dates are documented in the preface to the SOPs 1 Safety Safety should be a primary concern during monitoring activities Recommended safety practices include but are not limited to e Follow all traffic regulations e Be aware of surrounding conditions 1 e weather animals plants and take appropriate precautions e Park safely on the side of highways wear safety vests and use vehicle blinkers or safety cones e Aminimum of two people should be present on boat sampling excursions and all personnel should wear personal floatation devices e If someone feels that a station is unsafe the coalition coordinator should be notified to discuss possible alternate sampling ideas e Never wade into high water or swim to collect samples 2 Quality Assurance During Sample Collection and Transport The purpose of collecting samples is to obtain a representative portion of the medium being evaluated Proper sampling procedures and the handling of samples after collection cannot be
76. ndicated by the method These ideal ranges are defined in the method as Fecal coliform or Enterococcus bacteria 20 60 colonies Total coliform bacteria 20 80 colonies B1 Countable membranes with less than 20 colonies Reported value is estimated or is a total of the counts on all filters reported per 100 ml B2 Counts from all filters were zero The value reported is based on the number of colonies per 100 ml that would have been reported if there had been one colony on the filter representing the largest filtration volume reported as a less than value B3 Countable membranes with more than 60 or 80 colonies The value reported is calculated using the count from the smallest volume filtered and reported as a greater than gt value B4 Filters have counts of both gt 60 or 80 and 20 Reported value is estimated or is a total of the counts on all filters reported per 100 ml B5 Too many colonies were present too numerous to count TNTC TNTC is generally defined as 7150 colonies The numeric value represents the maximum number of counts typically accepted on a filter membrane 60 for fecal or enterococcus and 80 for total multiplied by 100 and then divided by the smallest filtration volume analyzed This number is reported as a greater than value B6 Estimated Value Blank contamination evident B7 Many non coliform or non enterococcus colonies or interfering non coliform or non enterococcus growth present In this competiti
77. ntymgr bladenco org Appendix III continued Chris May Cape Fear Council of Governments 1480 Harbour Drive Wilmington NC 28401 910 470 6609 cmayOcapefearcog org Cape Fear Resource Conservation Dev Joe Ann Carter Harrelson Center 201 N Fourth St Suite 210 Wilmington NC 28401 910 763 6611 Bill Memory Columbus County Board of Commissioners 114 Memory Plaza Whiteville NC 28472 910 642 5824 memorycompany whitevillenc net James Merritt UNCW Center for Marine Science 5600 Marvin K Moss Lane Wilmington NC 28409 910 962 2303 merrittj uncw edu Sidney Miller Triangle J Council of Governments PO Box 12276 RTP NC 27709 919 558 9392 smiller tjcog org Laura Padgett City of Wilmington Councilwoman 210 Castle Street Wilmington NC 28401 910 762 0542 lwpadgett bizec rr com Nancy Preston NC Coastal Land Trust 131 Racine Drive Suite 101 Wilmington NC 28403 910 790 4524 nancy coastallandtrust org Ed Kreul International Paper Riegelwood Mill 865 John L Riegel Road Riegelwood NC 28456 910 362 4883 Edward kreul1 ipaper com Jim Gregson NC DENR Regional Water Quality Supervisor 127 Cardinal Drive Ext Wilmington NC 28405 910 799 8704 Todd Walton NC State Ports Authority PO Box 9002 Wilmington NC 28402 910 251 5678 Todd_walton ncports com Gary Long Fortron Industries 4600 Highway 421 North PO Box 327 Wilmington NC 28402 910 341 3152 Ken Vogt Cape Fear Public
78. ogram Sampling Coordinator The data are then sent to each NPDES Subscriber of the LCFRP and other interested parties Questions about the data are referred to the Program Research Director for re evaluation or clarification which may include re analysis by the contract laboratory If no questions arise the data are then sent to the NC Division of Water Resources Coalition Coordinator NCDWR for entry into the EPA STORET system See page 2 this section for data flow chart A data status sheet is checked to ensure that data is sent to the proper entities on a timely basis see page 3 A data exclusion sheet will be maintained at the Aquatic Ecology Laboratory for the purpose of reporting and explaining MOA required samples that were not collected see page 4 Any data exclusions will be reported and explained in the annual LCFRP report to NCDWR per the MOA A copy of all data is kept on file in the UNCW Aquatic Ecology Laboratory An annual report detailing findings during the period from January to December is written by the scientists each year see Section 12 Section 10 Page 2 of 5 Version 6 May 2014 Lower Cape Fear River Program Data Flow Sheet Laboratory and Field Data available UNCW QC Check Valid Resolved Refer to Contract Lab Resolved Not resolved Data entry double check Data Excluded Monthly Comprehensive sheets sent to Program Subscribers Resolved Yes Refer to Contract La
79. on in this Agreement by providing 60 days written notice to the PERMITTEES the DWQ Coalition Coordinator s the appropriate DWQ Regional Office and the DWQ NPDES Unit The monitoring requirements contained in the individual NPDES permit shall become effective immediately upon such cancellation or termination In the event a permit holder terminates or cancels its participation in this Agreement the PERMITTEES may request that DWQ review the monitoring plan described in this Agreement for a possible reduction in sampling effort or requirements IN WITNESS WHEREOF the parties have caused the execution of this instrument by authority duly given to be effective as of the date executed by the DWQ DIVISION OF WATER QUALITY LOWER CAPE FEAR RIVER PROGRAM By CAG Chris May By b Director Chairman Division of Water Quality Lower Cape Fear River Prosram Date 4 30 d LCFRP MOA Page 3 of 17 LCFRP PERMITTEE SIGNATURES Permittee NPDES Number DAK Americas LLC NC0000663 Invista S aR L NC0001112 Global Nuclear Fuels NC0001228 Americas Carolina Power and Light CP amp L d b a Progress Energy Carolinas Inc NC0001422 Sutton Steam Electric Plant International Paper Company NC0003298 Riegelwood Mill LCFRP MOA 0 erating Director Signature T ace Y E Rick Bayless Environmental Health and Safety Manager Shawn O Connor Environmental Specialist Mark Frederick Plant Manager
80. oring Program e Research Director Dr Michael A Mallin Aquatic Ecology Lab at UNCW e Benthic Research Coordinators Dr Martin Posey Benthic Ecology Lab UNCW Troy Alphin Benthic Ecology Lab UNCW e Sampling Coordinator Matthew McIver Aquatic Ecology Lab at UNCW Section 4 Page 1 of 1 Version 6 May 2014 Section 4 Scientists Scientific staff 1s hired on a consensus basis between Dr Michael Mallin and Dr James F Merritt Persomnel are hired on the basis of appropriate education skills field and laboratory experience Curriculum vitae for these personnel are included in Appendix VI of this document A list of personnel dates of employment and each employee s signature is maintained in the AEL UNCW The list is used to verify signatures and initials on data sheets and other LCFRP documents Non program scientists in the Aquatic Ecology Laboratory routinely assist with fieldwork analysis and data entry The signatures of these employees are also included Below is a list of personnel working in the AEL UNCW at the time of this revision Lower Cape Fear River Program Scientists Dr Michael Mallin Science Director 1994 present n a r effi i a ei e BH ai Be gt A m r s e Z E ue d AE F Era e z E Matthew Mclver Sampling Coordinator QAQC 1994 present Ad TAES AAA de d a V V p Lk Aquatic Ecology Lab Scientists dd n Eens PhD 2009 present dh x A Id NOUS Aion Rob
81. ovide environmental education about the basin targeting point and non point source contributors and produce reports to identify changes or trends Develop consolidate and maintain a data base on the Cape Fear River Basin including historical and current data and make data available to public and private requestors including regulatory agencies Section 3 Page 1 of 1 Version 6 May 2014 Section 3 Program Organization Executive Director Dr James Merritt UNCW Center for Marine Science Advisory Board Appendix III e Determines policy for the program e Consists of representatives from citizen s groups academia local government industries the business community and regulatory agencies Technical Committee Appendix IV e Developed and oversees the monitoring program and scientific aspects of the program e Consists of representatives from UNCW the NC Division of Water Resource s Monitoring Coalition Program the NC Division of Marine Fisheries the Cape Fear Public Utility Authority the US Army Corps of Engineers technical representatives from industries New Hanover County US Geological Survey and other interested organizations and municipalities QA QC Subcommittee Appendix II e Asubcommittee of the Technical Committee created to develop and maintain a Quality Assurance Quality Control program to ensure scientific accuracy and precision and compliance with the Memorandum of Agreement Appendix I Research and Monit
82. p DAB the probe membrane to remove moisture and loosely screw on cap with on loosely with a wet sponge inside 2 Enter the Dissolved Oxy menu then enter DOsat Barometric pressure is entered in mmHg mmHg inHg x 25 4 and recorded on the calibration worksheet Press to begin calibration 3 Observe DO parameter display for about 15 minutes and when this value has stabilized record this pre cal value and the temperature value on the calibration worksheet then press lt If the calibration was successful Calibrated will appear at the top of the screen Record the cal saturation value and the DO charge on the calibration worksheet The DO charge should be between 50 and 50 if not the probe may be bad consult the maual Press Esc thrice to return to the Main menu highlight calibrate and press to enter calibration menu B pH Calibration If expected field pH values will fall between either 4 7 or 7 10 then you can perform a 2 point calibration with the two buffers that bracket your values In the case of potential field values spanning 4 10 you should perform a three point calibration using 7 buffer first then 4 and 10 1 To begin the pH calibration select the ISEI pH option from the Calibrate menu then select the 2 Point or 3 point option The display will prompt you to enter the first pH 2 Put pH 7 buffer in the calibration cup medicine dosage cup and slip over the pH probe and the temperature thermistor making sure
83. permit holders with the one exception of performing upstream and downstream water quality monitoring Subsequent to the execution of this Agreement the DWQ will issue a letter to each permittee notifying the permittee that the instream monitoring requirements of its permit are not effective for as long as this Agreement is in place and the permittee remains a party to this Agreement The PERMITTEES will contract for the performance of the monitoring activities described herein with a DWQ certified contract lab organization or agency who shall subcontract as necessary with a laboratory appropriately certified by DWQ for the required analysis Sample collection and field measurements will be made by the PERMITTEES the contractee or a sub contractee who will act as agent s of the PERMITTEES Each permittee has the right to review and comment on work data or reports prepared by any contractee on behalf of the PERMITTEES prior to its submission to DWQ and to notify DWQ of any objection or disagreement with any portion of the work data or reports Unless such notice is made within thirty 30 days of submission to DWQ it shall be deemed to be waived and the work data and reports submitted shall be deemed to be approved by the PERMITTEES It will be the responsibility of the PERMITTEES or their contractee to coordinate the collection and analyses of the water quality monitoring data at the locations parameters and frequencies specified in Appendi
84. pouneg ug weisde EN 00105868 f f ME I J9AOUEH TP uis uorquie OCT dl uojduruA 18 19 f e JOAOURH Toe I dLMM ys E _ TT dLAAM 9ITIAIeH Sup em rmg Ju W E EEN JoAOUEH dinis da youn uoj utuhrAA Je L000 0 0 9 pL 81 OYIM MON l6 6 L 98ST PE ueqm ALAM AD Prus sug 1 ON pue LIT SA Je 39er qus HD OS 0000264 n SZS 1JAQUBH SE SALMA OLOGSIYSL AM I SR S LY JOAQUEH s DE a dyma uiniuioJq Susto QUART ASEO P WC q 0 STT J pu LL OS dI MM Med ing MES ING ye p 199 1 Me3un W N N W JNOH IN W SO Z000 0 0 J t p2 81 OMIM Dia 0cc6 LL Lt9S vt sup ws juaique dma 8 Ie ZEE SA H 4991 Hr 1198 000027564 j COLO l bL lopuo 6 LL ECOS me3 ng sd A es donat Mag 0000056g IN W W W W W SO 000 0 0 Jot b 81 OMIM puod 8vt6 LL d IMM q san CHET WS Je 1991 EE Ae8mg 00000 H IN L000 0 0 EEE omm spuaq iseLLL coste us o1yjuag we ONV 00006r6a pisi o dej 1u c ON Je 991 ejosuy N W W LO00t0t die OYIM uydng vI86 LL vcCCL ve RANG atl s OUT 00009v64 ME E PPE D arm owm sdn Ju LEON 1024921 ustppos am T Be vam mE CNS l T 9SE E AA ne ee 0t cui teg Sd LAAM XUZ Klaue IN W Wo4 NW W SO 000 0 0 I b2 81 OYIM yang 2298 LL 1086 vt pue sit PIOJO suq JU QOLT US 149A1Y 1894 ade JN Uvs 00516164 Hp HD PW wainsva sse OH Doran Auno PPPP PD pppp pp siuounuo uongs uenduosoquogeso dai 9qUnN AO a mens mas g E E EE EE
85. ps Wallace WWTPI 347224 77 9814 B9030000 B9050025 B9050100 B9090000 Duplin B9130000 Panther Branch Creek nr Faison Duplin Goshen Swamp at NC 11 and NC 903 nr Kornegay NE Cape Fear River SR 1700 nr Sarecta B9191000 Duplin B9191500 Duplin B9430000 Duplin B9460000 Duplin alla benthic stn Angola Creek at NC 53 nr Maple 34 6562 77 7351 1 Ce CH B9490000 Pender T LCFRP MOA Revision 2 18 59 18 63 18 63 18 63 18 71 18 71 18 74 19 18 74 29 03030005 03030005 03030005 03030005 03030005 03030005 03030006 03030006 03030006 03030006 03030006 03030006 03030006 03030005 03030005 03030005 03030007 03030007 03030007 03030007 03030007 03030007 03030007 Field Measurements Stream Class Lab wl Chlorophyll a Coliform L popa D ME M C Sw February 26 2013 APPENDIX A LCFRP MONITORING PLAN Table A 1 LCFRP Sampling Stations Parameters and Sampling Frequency Revised Latitude Longitude Station Number Location Description Station Comments County Region dd dddd dd dddd DWO ambient stn dns Nene dius ro EE 34 3637 77 8965 i WIRO d WWTP NE Cape Fear River Nr Below GNF and Arteva New WWTPs 34 3171 77 9538 Em WIRO Wrightsboro B9720000 Smith Creek at US 117 and NC 133 Dns Smith Ck WWTP urban 342586 77 9391 New WIRO at Wilmington runoff Hanover Br
86. re out of control The sample is non homogenous e g VOA soil The reported value is the lower value of duplicate analyses of a sample N Presumptive evidence of presence of material estimated value This code is to be used if Nl The component has been tentatively identified based on mass spectral library search N2 There is an indication that the analyte is present but quality control requirements for confirmation were not met i e presence of analyte was not confirmed by alternate procedures N3 This code shall be used if the level is too low to permit accurate quantification but the estimated concentration is less than the laboratory practical quantitation limit and greater than the laboratory method detection limit This code is not routinely used for most analyses NA This code shall be used if the level is too low to permit accurate quantification but the estimated concentration is less than the laboratory practical quantitation limit and greater than the instrument noise level This code is used when an MDL has not been established for the analyte in question N5 The component has been tentatively identified based on a retention time standard Elevated practical quantitation limit PQL due to matrix interference and or sample dilution Holding time exceeded These codes shall be used if the value is derived from a sample that was received prepared and or analyzed after the approved holding time r
87. resents a practical and routinely achievable detection level with a relatively good certainty that any reported value is reliable APHA AWWA WEF 1992 Standard Methods for the Examination of Water and Wastewater 18 ed APPENDIX C DATA FORMAT AND REPORTING REQUIREMENTS LCFRP MOA Page 14 of 17 Data Format for Monthly submittals Table C 1 provides the required data submittal spreadsheet format Do not use commas tabs pipes or other common file delimiters anywhere in the table The first row should contain the column headings only Column headings must include appropriate information on measurement units mg l ug l cfu 100ml etc The second row must contain the method code It is very important that the format of the headings and the number and order of columns is consistent among all monthly submissions The DWO station number must be provided e g B6140000 An additional column containing the location description is acceptable as long as it is consistently included Include a comment column for describing pertinent information related to the sampling event or specific samples Ensure that there are no missing values for station date time and depth Place all remark codes in a separate column as demonstrated in Table C 1 If there is no result for a particular parameter leave the cell blank Screen all data for inappropriate or improbable values such as a pH of 21 2 Annual Report The LCFRP is required to submit an annual report by
88. rine Science Instructor May 1994 2003 part time Carolina Ocean Studies Carolina Beach NC Lead estuarine and offshore marine education field trips for high school and middle school students Official Biologist September 1993 Present South Brunswick Islands Chamber of Commerce Shallote NC South Brunswick Islands Annual King Mackerel Fishing Tournament Inspect fish for compliance with tournament rules Laboratory Instructor August 1992 May 1994 University of North Carolina at Wilmington Department of Biological Sciences Technical Skills SCUBA Openwater I certified UNCW NITROX certified UNCW Boat Captain certified vessels lt 25 feet Computer Skills Microsoft Excel Word Access Power Point US EPA STORET Paint Shop Pro Web Page Design Desktop scanner applications Netscape Navigator Peer Reviewed Articles Tavares M E M I H Spivey M R McIver and M A Mallin 2008 Testing for optical brighteners and fecal bacteria to detect sewage leaks in tidal creeks Journal of the North Carolina Academy of Science 124 91 97 Mallin M A L B Cahoon B R Toothman D C Parsons M R McIver M L Ortwine and R N Harrington 2007 Impacts of a raw sewage spill on water and sediment quality in an urbanized estuary Marine Pollution Bulletin 54 81 88 Mallin MA M R McIver H A Wells D C Parsons and V L Johnson 2005 Reversal of eutrophication following sewage treatment upgrades in the New River Estuary North Caro
89. rtment of Environment and Natural Resources Division of Water Resources NCDWR coalition coordinator for review This QA QC manual Annual Reports associated scientific publications and relevant data are distributed to interested members of the LCFRP Advisory Board Appendix III and Technical Committee of the Lower Cape Fear River Program as well as other agencies and the public Section 2 Page 1 of 2 Version 6 May 2014 Section 2 Program Goals History and Mission Statement l Program Goals The Lower Cape Fear River Program LCFRP Is an un incorporated non profit association The goal of the LCFRP is to develop a science based understanding of processes that control and influence the ecology of the Cape Fear River and to provide a mechanism for information exchange and public education In an attempt to meet this goal a basin wide coordinated physical chemical biological and water quality monitoring program has been developed and implemented The Dischargers Association 1 e Subscribers contract with the Aquatic Ecology Laboratory at UNCW to carry out monitoring and research activities as specified in the Memorandum of Agreement Appendix I between the dischargers and the North Carolina Division of Water Resources Monitoring sites and parameters are strategically located and established such that in stream monitoring is more efficient effective basin oriented and yields more usable data than in stream monitoring from individual d
90. s at stations with required chl a sampling should be collected as photic zone integrated samples using the lab line See section A for information on using the lab line Section 6 Page 3 of 7 Version 6 May 2014 G Turbidity Laboratory turbidity samples are collected in oz plastic bottles hold time 1s 48 hours Field turbidity not a state certified parameter 1s measured with the YSI multi parameter water quality meter Prior to leaving for the field the meter 1s calibrated at 0 NTU Upon return to the laboratory the meter is checked using the O NTU standard 5 Field Parameters e Water temperature dissolved oxygen specific conductivity salinity field turbidity and pH NC DWR certified parameters are measured using a YSI multi parameter water quality meter MPWQM e Measurements are taken near the surface at approximately 0 15 m and near the bottom of the water column at N11 AC DP BBT IC NCF6 NAV HB BRR M61 M54 M35 M23 and M18 and at the surface for all other stations e Data are recorded on pre printed field data sheets e Probes are calibrated according to manufacturer s instructions and DWR certification guidelines before each sampling trip and are checked upon return to the lab see Section 8 for calibration information Calibration worksheets are completed and attached to the field data sheet for each sampling trip e Adissolved oxygen calibration drift check 1s performed during each trip as indicated on the
91. s lowered to the water surface and rapidly lowered so that the bottles fill below the surface Figure 6 4 Lab Line Integrated Sampler This device is constructed of plastic rope and a spring Its purpose is to collect an integrated sample of water over a given depth The bottle plugs are put into the holes on the top the instrument is lowered to twice the secchi depth a quick pull on the line pops the plugs out and as the instrument is raised to the surface 1t fills with water over the entire depth range Section 6 Page 6 of 7 Version 6 May 2014 Typical Field Equipment Checklist Field Equipment Checklist for Lower Cape Fear River Program Sampling Estuary Boat Trip NAV HB BRR M61 M54 M35 M23 MIS 1 Quart Bottle Unpreserved per station TSS 1 Quart Bottle w H5SO per station Nutrients gt a NOt ane we wN green plastic tie to remind for Lab Line use 1 Pint bottle per station for NAV M61 M18 Lab Turbidity 1 Coliform NAV HB Enterococcus M18 M23 M35 M54 M61 BRR 3 125 ml Chlorophyll a Bottles per station 1 250 ml Chl a bottle for MIS 125 ml Chl a bottle for M61 Sample with Lab Line Label with green tie _ Float Plan _ Rain Gear Float Coats _ YSIMPWQ Probe _ Red Bucket YSI Guard Small Bucket YSI Cap YSI Line 15m ___ Lab line sampler __ 2 Weights YSI and Light Meter __ Light Meter ___ 3 Large Coolers Field Notebook and Pens Ice the Coolers _ Field Data Sheets __ Se
92. s the use of equations for uncorrected chlorophyll a The light source is a blue lamp which passes through a filter that allows the wavelengths at 436 nm to excite the sample The light emitted by this excitation then passes through a filter that allows wavelengths at 680 nm to pass on to a photo multiplier which quantifies this light II III Section 9 Page 2 of 12 Version 6 May 2014 Supplies Instruments 125 250 mL light proof plastic bottles Filtration Tunnel 25 mm base 50 mL graduated cylinder Vacuum pump with gauge 25 mm glass fiber filters nominal pore size 0 7 micron Forceps Aluminum foil Permanent marker Freezer capable of 20 C Plastic airtight container with desiccant Certified thermometer for freezer Racks of 50 mL centrifuge tubes with screw caps polypropylene Masking tape Arrow JR4000 overhead electric stirrer Kontes Potter Elvehjem tissue grinder size 22 pestle 2 10 mL pipette with tips 90 acetone solution 100 mL beaker 100 acetone solution VWR BDHIIOI 19L Waste acetone container Kimwipes Fisher 06 666A Squirt bottles for DI water Turner 10 AU Fluorometer with filter set10 040R Fluorometer cuvettes Sample Collection and Filtration 1 A chain of custody sheet COC 1 2 1 should be filled out for each set of samples 2 Samples should be collected in 125 mL light proof bottles a 250 mL bottle should be used for samples requiring bottle duplicates 1096 of samples
93. sity of North Carolina at Wilmington Wilmington N C Mallin MA M R McIver M I H Spivey and B Song 2009 Environmental Quality of Wilmington and New Hanover County Watersheds 2008 CMS Report 09 03 Center for Marine Science University of North Carolina at Wilmington Wilmington N C Mallin M A and MR McIver 2009 New River Estuary Water Quality 2007 UNCW CMS Report 09 02 Report to Environmental Management Division US Marine Corps Camp Lejeune N C Center for Marine Sciences University of North Carolina Wilmington Wilmington N C Mallin MA M I H Spivey and B Song 2009 Sources of Fecal Bacterial Pollution to Upper Pages Creek N C Report to Coastal Planning amp Engineering of North Carolina Inc UNCW CMS Report 09 01 Center for Marine Science University of North Carolina at Wilmington Wilmington N C Mallin M A 2006 Wading in Waste Scientific American 294 52 59 Presentations to Professional Organizations 187 total Editorial Board Member Journal of Experimental Marine Biology and Ecology Coastal and Estuarine Science News CESN Appendix VI continued Matthew R Mclver Present Position May 1995 present Research Specialist Laboratory Manager Aquatic Ecology Laboratory Center for Marine Science University of North Carolina at Wilmington 5600 Marvin Moss Lane Wilmington N C 28409 Telephone 910 962 2357 office 910 686 0018 home mcivermOuncw edu Education Master of Science Marine B
94. t the end of the do probe is in the water This keeps the probe end from drying out during storage which may cause small cracks to form Conductivity Leave the conductivity probe on the sonde during long term storage Section 8 Page 5 of 5 Version 6 May 2014 WS 1 1 1 YSI Calibration Worksheet Project Date Analyst Signature sonde Number Dissolved Oxygen Post Sampling Check AEL Probe pess fail eee 50 4 2 AEL Probe Cell Constant 5 0 0 5 Calibration Lo pu Check sandara Jegen yes no Post Samping Check oes mo win tete ye no Specific Conductivi Turbidity AEL Probe amp Standard Value Calibration Time NTU kal Reading After Cal Resding post Samping chex JL kein am yes n Section 9 Page 1 of 12 Version 6 May 2014 Section 9 Standard Operating Procedures for AEL Lab Analyses Several parameters are analyzed by the Aquatic Ecology Laboratory at the UNCW Center for Marine Science The MOA requires Chlorophyll a analysis using NC DWR Certified methodology employs grinding of the filter at three LCFRP stations The Aquatic Ecology Laboratory Is NC DWR certified to perform this analysis Appendix VII Chlorophyll a is also analyzed for all LCFRP stations using a non grinding method Biochemical Oxygen Demand 5 and 20 day which is not required by the MOA is analyzed at six LCFRP stations using non certified methods A
95. the field The acceptable range is within 5 of the standard value D Turbidity Although there is no certification for turbidity a check using 0 NTU standard is performed Calibration Standards MPWOQS calibration is only good if the calibration standards are good The AEL orders most of its calibration standards from EXAXOL Chemical Corporation 14325 60 St N Clearwater FL 33760 727 524 7732 Expiration dates should be noted and highlighted A calibration standards use log LS 1 9 1 is maintained and should be filled out as standards are opened and discarded YSI Cleaning Maintenance and Storage A Cleaning The YSI sonde probes may be cleaned periodically using SOP 3 5 Record date of cleaning on the YSI cleaning log LS 3 5 1 B Maintenance Maintenance procedures for each probe are outlined in the YSI user s manual The dissolved oxygen membrane should be changed on a regular basis otherwise most probes do not need regular maintenance C Storage YSI sondes are stored by hanging from the stainless steel hooks under the shelving The storage cup containing a damp sponge is screwed on snug When a sonde will not be used for more than several weeks long term storage procedures are used pH Remove the pH probe store in the storage vial containing Plug pH port Turbidity Remove the turbidity probe and store dry Plug turbidity port Dissolved Oxygen Leave the probe on the sonde Put distilled water in the storage cup so tha
96. to Appendix VII Appendix VI is now Scientist CVs Appendix VII Lab Certification information previously in Appendix VI Appendix VIII Lab Methods and SOPs previously in Appendix I Section 1 Page 1 of 1 Version 6 May 2014 Section Purpose of Manual This manual describes Quality Assurance and Quality Control QA QC procedures developed for the Lower Cape Fear River Program LCFRP in association with the Aquatic Ecology Laboratory AEL at the University of North Carolina at Wilmington s Center for Marine Science UNCW CMS The primary goal of this quality assurance quality control plan is to provide for the collection analysis and reporting of consistent and valid scientific data These procedures are reflective of the requirements of the Memorandum of Agreement Appendix I the Monitoring Coalition Program Field Monitoring Guidance document from the North Carolina Division of Water Resources Coalition Monitoring Program http portal ncdenr org web wg ess eco coalition and the recommendations of the LCFRP QA QC Subcommittee Appendix II Periodic review 1s performed by the Quality Assurance Quality Control Subcommittee and any changes are forwarded to the LCFRP Technical Committee Appendix IV for approval If at any time other than the periodic review the Research Director determines changes are necessary he will forward the changes to the Technical Committee If necessary the changes are sent to the North Carolina Depa
97. to remove accumulated grunge Record the fluorometer values and other appropriate information on the chl a bench sheet BS 1 2 1 Room temperature and sample temperature should be recorded on the bench sheet VI Computation of Chlorophyll a Concentration I Chlorophyll a concentration in ug L ppb is calculated using this formula Cs extract volume mL x DF sample volume mL Cs uncorrected Chlorophyll a concentration in whole water sample extract volume volume of extraction acetone DF dilution factor volume of extract total volume sample volume volume of whole water filtered VI Quality Assurance Quality Control I LRB s are analyzed with each set of filters An LRB consists of di H2O that 1s filtered the same as samples after all samples have been filtered Elevated LRB values indicate contamination at some point during the procedure If the LRB value is gt 10 of the analyte value the samples are considered contaminated and should be discarded A Quality Control Standard Secondary Check Standard of a source other than the calibration standard 1s measured at the beginning and the end of a batch of samples The measured value should be within 10 of the actual concentration 1f not the fluorometer may need to be recalibrated See SOP 1 7 Fluorometer Calibration for details on the Secondary Check Standard The linear dynamic range has been determined for the fluorometer and the upper detection limit U
98. tra water in the bottle will form water seal above the stopper in the neck of the bottle which the plastic cap will keep from evaporating A little di water can be added to get a good water seal Place in incubator at 20 C BODS5 and BOD20 Readings Calibrate DO and pH meters as described above Record incubator temperature on bench sheet use certified thermometer Measure DO and pH of each sample rinsing probe and dabbing dry between samples Record values in tenths on bench sheet If performing 5 day BOD measurement re seal and cap bottles and place back in incubator for BOD20 incubation Ensure no air bubbles are trapped in the bottle after the stopper is replaced After 20 day BOD has been measured the bottles are emptied and rinsed two times with hot tapwater Place about 100 ml of lab soap solution into bottle shake for 10 seconds rinse with di water and allow to air dry for storage The BOD bottle storage box 1s labeled for each bottle by number If the BODS value is at or above 5 the sample must be diluted by 50 before being placed back in the incubator Previous experience shows that a sample that has a BOD value of 5 after the fifth day will use all its available oxygen before day 20 Therefore without dilution the 20 day reading will be inaccurate VIII References American Public Health Association Standard Methods for the Examination of Water and Wastewater 20 Ed 1998 Pg 5 2 NCDEHNR 1993 Long Term Bioc
99. uck Masters Leen A 2011 present dX ku iy IMA Al Ai Y ut pe K i Mary G race tenon Masters Student 2013 present a e r P E z a F Za F au ug j p Fa Be j 1 mji z D ed af d F E fff A 1 LZ f l A Y Na CG K Lt L Lona d ech HF D E tos P w E La ei e y Ti har Laf Section 5 Page 1 of 3 Version 6 May 2014 Section 3 Station Descriptions and Sampling Schedule Samples are collected at 32 stations required by the 2011 Memorandum of Agreement with North Carolina Division of Water Resources Appendix I Table A 1 Revised and one additional research station added by the AEL Table 5 1 and Figure 5 1 Samples are collected monthly requiring five field days There are nine estuarine stations sampled by boat five riverine stations sampled by boat and nineteen stations sampled from highway bridges and culverts Additional sampling 1s carried out during summer as required by the Memorandum of Agreement Table 5 1 Station information for the Lower Cape Fear River Program Collected by Boat NC11 B8360000 Cape Fear River at NE 11 nrEast Below Lock ane Dam 1 i Bladen 34 3969 78 2615 WSIVSw 03030005 Arcadia water entering lower basin LVC2 B8441000 Livingston Creek at Momentive DWR ambient SIND Downstream Columbus 34 3353 782011 CSw 03030005 Walkway nr Acme of Momentive AC B8450000 oe NI A TUE Columbus 34 3555 78 1794 C Sw 03030005 Landing nr Acme station B8465000 Cape Fear River at SS a nr Hooper AT DAK intak
100. unswick River dns NC 17 at park Park access from SR 133 B9790000 nr Belville dns Belville WWTP 34 2214 77 9787 Brunswick WIRO B9580000 B9670000 B9795000 Cape Fear River at Channel Marker Dns Wilmington Southside 34 1393 77 9460 New WIRO 54 WWTP Hanover Cape Fear River at Channel Marker Dns Wilmington Northside New B28000900 61 at Wilmington WWTP DWQ ambient stn k ATTAT Hanover WIRO Cape Fear River at Channel Marker e B9850100 35 Ups Carolina Beach WWTP 34 0335 77 9370 Brunswick WIRO B9910000 SE Fear River at Channel Marker au Carolina Beach WWTP 33 9456 77 9696 Brunswick WIRO B9921000 Fear River at Channel Marker O of Cape Fear 339130 78 0170 Brunswick WIRO Index 18 74 47 5 18 74 52 5 18 74 63 18 77 18 71 18 71 18 71 18 87 5 18 88 3 5 8 Digit HUC 03030007 03030007 03030007 03030005 03030005 03030005 03030005 03030005 03030005 Stream Class B Sw C Sw C Sw SC SC SC SC SA HQW SC Field Measurements M M 2SM M M M 2SM M 2SM M 2SM M 2SM M 2SM Lab s Chlorophyll a Coliform DENEN Lal Jal Is nl Jul xl LS et iw x KS x js mW ei Lx paa Iw al EOM EOM M M E lt E Sr lt z lt lt lt lt B EJE B Field Measurements include Temperature Dissolved Oxygen pH and Conductivity M Monthly M 2SM Monthly with twice monthly summer samplin
101. ve situation the reported value may under represent actual density Note A B value shall be accompanied by justification for its use denoted by the numbers listed above e g Bl B2 etc Note A J2 should be used for spiking failures LCFRP MOA Page 12 of 17 Data Remark Code Definition This code applies to most probable number MPN microbiological tests 1 No wells or tubes gave a positive reaction Value based upon the appropriate MPN Index and reported as a less than lt value 2 All wells or tubes gave positive reactions Value based upon the MPN Index and reported as a greater than gt value Note A BB value shall be accompanied by justification for its use denoted by the numbers listed above ep BB1 BB2 etc Total residual chlorine was present in sample upon receipt in the laboratory value is estimated Generally applies to cyanide phenol NH TKN coliform and organics A single quality control failure occurred during biochemical oxygen demand BOD analysis The sample results should be used with caution G Gl The dissolved oxygen DO depletion of the dilution water blank exceeded 0 2 mg L G2 The bacterial seed controls did not meet the requirement of a DO depletion of at least 2 0 mg L and or a DO residual of at least 1 0 mg L G3 No sample dilution met the requirement of a DO depletion of at least 2 0 mg L and or a DO residua
102. x A Sample collection and field measurement methods shall follow procedures outlined in Appendix B The PERMITTEES or their contractor shall submit the water quality data to DWQ using the format described in Appendix C preferably Microsoft Excel 2000 or a subsequent version or the equivalent The Permittees or their contractor shall submit the water quality data to DWQ within 90 days of the end of the month in which the sampling was performed All data sheets shall be archived by the PERMITTEES or their LCFRP MOA Page 1 of 17 contractee for a period of 5 years PERMITTEES or their contractee shall submit an annual written report of its compliance or non compliance with the monitoring requirements as specified within this Agreement no later than April 30 each year that this Agreement is in effect The report shall include the NPDES permit number of each actively participating permittee the cause of any non compliance with stipulations of the Agreement any remedial action taken and the probability of meeting the next schedule requirements Additional requirements for the reports are outlined in Appendix C Two signed copies of these and any other reports required herein shall be submitted to the DWQ Coalition Coordinator s at1621 Mail Service Center Raleigh NC 27699 1621 Failure by PERMITTEES or their contractee to collect the water quality data as described in this Agreement or to provide the data to DWQ in the required format may result in
103. y DWQ Such amendments may be entered into at any time The parties may also desire to allow Dischargers 1 who subsequent to the date of this Agreement receive NPDES permits within the Lower Cape Fear River Basin or 2 who have NPDES permits within the Lower Cape Fear River Basin but are not parties to this Agreement to enter into this Agreement subsequent to the effective date hereof Any such changes can only be made by a written amendment to this Agreement agreed to by DWQ and a majority of the PERMITTEES then party to the Agreement The amendment shall be signed by PERMITEES primary contact and by DWQ and if appropriate by an authorized officer of any such Discharger who wishes to enter into the Agreement subsequent to the effective date hereof DWQ will consider modification of existing monitoring requirements for any such discharger similar to those in effect for the existing PERMITTEES Such amendments may be made at any time that this Amendment is in effect LCFRP MOA Page 2 of 17 This Agreement shall be effective until June 30 2016 unless extended by the consent of both parties Upon 60 days written notice DWQ or a majority of the PERMITTEES then party to the Agreement may terminate this Agreement for any reason Upon termination of this Agreement the monitoring requirements contained in the individual NPDES permits of the PERMITTEES shall become effective immediately An individual permittee may terminate and cancel its participati

quality assurance/quality control manual for the lower cape fear river

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