User`s Manual - Turner Designs
Contents
1. Station Hata Piu Erop Eny Spadki a name dssigad to ihs alation This vaus is paed onky Pita datalcepgar i Cun inning a pagam wih CesdOue i nsiuctons asi Eri pT steel BI Dmt 998 2300 Rev N Page 34 C3 Submersible Fluorometer 2 5 Next to Select the ComPort click the dropdown menu and select RS 232 Next select a Baud Rate of 9600 Fixed Then click Apply At this point you are done with the Device Configuration Utility Click on Disconnect papei Logger Combo Omi Momitor Pim Conirol card OS Satinge Editor Tarina E O e Eet atira rere or ppp nator Services acana RN Sei he cont RS k Station sms Species a name assigned to dhe station This value is read onky fiha draugar i Curantty running a progam wih Card uc ii metruchons 3 Connecting the C3 to the CR1000 3 1 The C3 uses RS 232 logic levels therefore communication between the CR1000 and the C3 must be on the RS 232 port of the CR1000 The configuration of this port is described in section 2 Connect the 8 pin female underwater connector end of P N 2200 150 to the C3 Connect 9 pin female DB9 end of P N 2200 150 to the male 9 pin DB9 on P N 2300 115 3 2 Between the C3 Communication and Integration cable P N 2300 115 and the CR1000 RS 232 port you must add a null modem device The image below shows the C3 instrument with the proper cables connected and the null modem device added 998 2300 Re2. 1 L7 H LE 176 i e ed a SA ee e e e e e e e e e e bats Pats eE OD DAD SS dS ed ed CICI ed dd Bd IEA icc sc ks ss aks oc sc us cs a r T T J j i ee ee ee a a S n e a nS ni ni mii SM nM nM nl Gl lls ni a a a gt ee A Rev N Page 38
3. 3 3 2 998 2300 C3 Submersible Fluorometer Data will now be corrected for temperature fluctuations The temperature compensation coefficients for each fluorophore are listed in the table below Fluorophore Coefficient Rhodamine 0 026 C Exponential in vivo Chlorophyll 0 014 C Linear Step 5 Save Calibration After calibration is complete select Save Calibration Data are now displayed as concentration estimates Raw Fluorescence Calibration Blank Subtracted Following steps 1 5 below will ensure values are reported as Blank subtracted Relative Fluorescence Units RFUB See Appendix B for recommended practices Step 1 Blanking Place the C3 Submersible Fluorometer in a blank solution Note A blank solution is a solution without the fluorophore of interest i e deionized water artificial water or filtered water Note Ensure the optical head is free of air bubbles Wait until Current RFU readings have stabilized then select the Set Blank button to set the blank value Wait for the blank value to be measured and set for all 3 gains Step 2 Selecting Units Select RFUB from the units dropdown menu Note Do not enter a value in the standard value box Step 3 Skip and go to Step 4 Step 4 Optional Temperature Compensation Follow same steps as the Direct Concentration Calibration procedure in Section 3 3 1 Step 5 Save Calibration After calibrat
4. 3 adjacent to the optics on the face of the instrument Each channel is factory set in the C3 Windows Software Note The channel labels can be modified in the C Soft software Recommended Measurement Practices The following steps will improve the accuracy and repeatability of your measurements especially at low concentration levels 1 Use a dark plastic or glass container for your standards and or samples Note Some plastics may fluoresce and might interfere with measurements If using a glass beaker place it on a non reflective surface preferably black Ensure that the sensor is gt 3 inches above the bottom of the container Ensure that the sensor is in the center of the container and has gt 2 inches clearance between the sensor and the inside surface of the container 5 If using a glass beaker check if the optical surface of the sensor is free of air bubbles Remove air bubbles if any are observed If using a dark plastic container run the wiper or your finger over the optical sensors to ensure the sensor heads are free of air bubbles Calibrated Sensor gt ae gt 2 inches all round Glass Beaker 998 2300 Rev N Page 26 C3 Submersible Fluorometer Appendix C Wiring Guide The C3 Submersible Fluorometer outputs digital data in three formats 1 Digital data that can only be read by C3 Submersible Fluorometer s software 2 Digital data in ASCII format that can be read by larger mu
5. Soft icon is located on the desktop Gor C Soft C Soft Note You may receive a warning message asking if you want to unblock messages select Unblock 3 1 3 Integration Interface Adapter Cable Installation 1 998 2300 Insert the USB Flash Drive and install the Adapter Cable driver Integration Interface_Adapter_Drivers exe onto your PC Make sure nothing is plugged into the Adapter Cable s connectors Plug the USB connector into a USB port on your computer and wait for hardware to automatically install Once USB is installed and ready to use right click the My Computer Icon Note For Windows Vista or 7 users open the start menu and right click on Computer Left click properties to open the properties window If running Windows XP or older versions of Windows click on the Hardware tab and then click Device Manager to open the list of devices installed on your computer Note For Windows Vista or 7 users the Device Manager link is located in the upper left part of the properties window you will not see a Hardware tab Rev N Page 9 C3 Submersible Fluorometer Scroll down and open the Ports heading from the list 8 The newly installed Adapter will be titled USB Serial Port followed by a COM port designation in parentheses for example COM 5 9 If the USB Serial Port s COM designation is labeled as any number from 1 6 your installation is complete Close all window
6. caion Atmospheric Offset Method 1 Step 1 Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero Step 2 Select Atmospheric offset only Step 3 Select Save Calibration button Depth values will now be scaled to the offset selected Rev N Page 17 C3 Submersible Fluorometer Atmospheric and Gravity Offset Method 2 Step 1 Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero Step 2 Select Atmospheric and gravity offset Enter the latitude Step 3 Select Save Calibration button Depth values will now be scaled to the offset selected 3 4 Log Setup Screen The Log Setup screen allows the user to configure a logging session set the mechanical wiper to its home position and enable logging The C3 Submersible Fluorometer can log over 60 000 lines of data Enter a log start date MM DD YYYY and time HH MM SS Enter the sampling interval HH MM SS The minimum sampling interval is 1 second fe ieee THR Hele riley faeries LSS Pei Gale Ifthe mechanical wiper is
7. fluorescence units RFU label will appear Rev N Page 12 C3 Submersible Fluorometer Sr ees slit a Bie Seegn 0 Pert Biip mm j Da Taha Tpipy bpm Gria Deri iia omy Sedge Bornia Dual Tim Teme nriran lee a sy L i i ine i k aE lem n Pa Cei eE IRA i 1 T 13 E Enia ie l k ami a T 3 id E T E Cn ei Tats Lie 1h Eel CTIE ECCLE T E i _ wicca fi H 3 Fsai 9 ea ieran Ea Haruai ami imi I raned Farr l Led eel EF Foes eed iB Gl a H a4 F s48e Bd lbs Waveform Screen Users can graphically view real time data for each sensor by clicking the Display Waveform box Channel names and units will be displayed on the Y axis Time and date will be displayed on the X axis Clicking the Autoscale box will automatically scale graphs Users may also rescale graphs by unchecking the Autoscale box and manually entering desired values for either Y or X axis ranges Data can be saved during waveform mode by clicking Start Data Capture Data files will be saved as csv format i ii COM Pe bek 66 MultkBenmsor Piatforn rege lren Dale ee ee brwi Cote depiel a pi iw I ima byien Ha ay O LEH i i _ a allo ote fre Da are 998 2300 Rev N Page 13 C3 Submersible Fluorometer Calibration Screen Users can calibrate sensors in either the direct concentration mode raw fluorescence mode or blank subtracted raw fluorescence mo
8. installed click the Wiper box to enable Select the number of revolutions from the dropdown box 1 10 revolutions All sensors will be wiped at specified revolutions before each measurement Note If users select revolutions that exceed sampling interval a warning message will be displayed 998 2300 Rev N Page 18 C3 Submersible Fluorometer Note Do not click the Wiper Box to enable wiper if the wiper is not installed x The sampling interna e too shart for the number of wiper rewolutions You must choose a longer sampling time less revoltons Of turn the wiper off Toi Maun Interval of Revs 1 10 11 20 l 21 30 2 31 40 3 4150 4 51 50 5 10 S50 0 Carmel After log set up is complete select Enable Datalog see section 3 3 Enabling Data Output Ready State A prompt will appear asking if user is ready to enable logging select Yes Note If data logging is set to start for a time in the past there will be a minimum delay of 100 seconds before startup after power has been supplied Otherwise wait until the data logging start time has been reached for data logging to begin WARNING As soon as data logging is enabled users will be unable to access C Soft settings This should be the last step before the C3 Submersible Fluorometer is deployed 3 5 Enabling Data Output Ready State The Data Output Ready State must be enabled for the C3 Submersible Fluor
9. should be increasing at a rate of 1 every 10 seconds The TimeStamp is the time of the measurement as kept by the CR1000 This timestamp is independent of the timestamp delivered by the C3 which is contained in the RawString field The RawsString field contains the serial string as delivered by the C3 SplitData fields are the parsed records from the C3 serial data string SplitData 1 represents channel 1 SplitData 2 represents channel 2 and SplitData 3 represents channel 3 SplitData 4 represents pressure which in this case Is 0 SplitData 5 represents the temperature measurement by the C3 in degrees C SplitData 6 SplitData 7 and SplitData 8 are fields that would be occupied if the CR1000 were interfaced to a Turner Designs C6 Multi Sensor Platform 7 PEROT L2 Datalogger Support Software ORTOOO CR1000 O ESM Fie Oetelogger Hebwerk Toch Help ku gF OD BASE oO Cocks Progam Morte ite Colet Date res sista patties Decks Aeg 2 gt AApebste kiera wm 015 Rechum 454 TimeStamp 143155 Rawing 10D 2461 Tita 1 35 Oe Traa 7144 SoleO ata 177 62 Crita Oe apinat 2461 Sonata HAM Tpit HARI Triana HARI Timer 414 3144 Epei 18 00 bhrises Her EFAA 4 6 For reference the serial data string coming from a C3 into a terminal program is displayed below 998 2300 seb Pee fafa ee fa e Pa Pa Pee Pan E T F
10. tPF 2Ed i 13 Prd Direct Gonmei Conme tign Cod Pari ATEH LG bo Sariai Bridge oC if Pause Clock Update o Catal grr Tira fore Otfanl Ewid Fuster ec Pub bus dies 77 ri ee Dhanin HE Security Code D Eira Paipa e Tide fs Dieta Fil Paths Datalegge Progam Statun C Cern phalecfPC200M CRO Statin dad Curent Pgri Punic ChCnng bee PCa A Public at CE Cataleg ging vi CRI Cie Cee Gel Po TCE ee Peries P regram 998 2300 Rev N Page 36 C3 Submersible Fluorometer 4 3 Successful compilation of the program by the CR1000 will result in a message box like the one below Click on OK The program was successfully sent to the datalogger CPU C3_C6 Datalogging_v2 CR1 Compiled in PipelineMode 4 4 Click on the Monitor Data tab Fy File Diuber Network Took Help Kom 3S OD pan 0 E Clocke Pregram Monitor Duta Coliect Data Dolah gaa Flare PP rrp j Di Daioh gyer Troe st og car b s id 1 24 Pra ee ac DATI fea Pd Direct Commect i Cob Aarh ATEH Saini Botched iy Pnet Tok pire Esad Piste 0 Poi bus Goins Tii Se muriby Tede D Eira kepam e Tawa fe Daia File Paita Piau Carpha ini P M pO Hndi Ciani Pregn punio OVC Aa mAAR CA Pubin C3_ CE Deegan vl CR Et oe ra bihi AC Td Serj Frj Fanera Pregn 998 2300 Rev N Page 37 C3 Submersible Fluorometer 4 5 The GUI will change to reflect the image below The RecNum field is representative of record number This value
11. Note Ensure the optical head is free of air bubbles Wait until Current RFU readings have stabilized then select the Set Blank button to set the blank value Wait for the blank value to be measured and set for all 3 gains Step 2 Standard Value and Units Place the C3 Submersible Fluorometer in a standard solution or use a solid secondary standard See Accessory Instructions on the USB Flash Drive for additional information Note Turbidity sensors do not have solid secondary standards Enter the known concentration value of the standard solution or solid standard in the Standard Value box If the concentration is not Known enter an arbitrary value Enter manually or select desired units from the dropdown menu Note Do not choose or enter RFUB if Direct Concentration Mode is preferred Step 3 Set Standard When readings in Current RFU box have stabilized select the Set Standard button Step 4 Optional Temperature Compensation Temperature compensation is optional it enables the C3 to compensate for changes in fluorescence due to varying temperatures as detected by the C3 s temperature sensor Temperature compensation is available for in vivo Chlorophyll and Rhodamine sensors only Temperature is displayed in degree Celsius Click on the Temperature Compensation box Select either in vivo Chlorophyll or Rhodamine from the dropdown menu Rev N Page 15
12. TURNER DESIGNS Reliable Instruments for an Unreliable World Submersible Fluorometer User s Manual January 30 2015 P N 998 2300 Revision N TURNER DESIGNS 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 C3 Submersible Fluorometer Table of Contents 1 Introduction 1 1 Introduction 1 2 Important Note Regarding the Use of Fluorescein Optics 2 Inspection and Setup 2 1 Instrument Checklist 2 2 Optional Accessories 2 3 Identification 3 C Soft Windows User Interface 3 1 Software Installation and PC Requirements 3 1 1 Minimum PC Requirements 3 1 2 Software Installation 3 1 3 Integration Interface Adapter Cable Installation 3 1 4 PC Software Interface 3 2 Software Operation 3 2 1 Settings Screen 3 2 2 Current Data and Calibration Screen 3 3 Calibration Procedures 3 3 1 Direct Concentration Calibration 3 3 2 Raw Fluorescence Calibration Blank Subtracted 3 3 3 Raw Fluorescence Calibration 3 3 4 Depth Calibration Optional Accessory 3 4 Log Setup Screen 3 5 Enabling Data Output Ready State 3 6 Downloading and File Management 4 Maintenance and Warranty 4 1 Maintenance 4 1 1 Rinsing 4 1 2 Care for the Bulkhead Connector 4 2 Warranty Terms 4 3 Warranty Service 4 4 Out of Warranty Service Appendices Specifications Recommended Practices Wiring Guide Linear Range Quenching and Temperature Considerations How to set up HyperTerminal for Digital ASCII Data Cap
13. de r firre Ci IME Lata han Ehr Curent AFU oa Ste l Sii SA rE ee ik rie bo He comet U Jepi Cer Ce ee De Targ Compani Sear nat p La Direct Concentration Mode Values reported in the Direct Concentration Mode are scaled to a predetermined standard value and blank subtracted The equation used to calculate concentrations after calibrating to Direct Concentration Mode is Standard Value units x Sample RFU Concentration units Standard RFU Raw Fluorescence Blank Subtracted Mode Values in the Raw Fluorescence Blank Subtracted Mode are noted as RFUB Itis important to note that these values are blank subtracted but are not scaled to a standard they are relative values Raw Fluorescence Mode Values in the Raw Fluorescence Mode are referred to as Relative Fluorescence Units RFU Itis important to note that these values are not blank subtracted or scaled to a standard they are relative values 998 2300 Rev N Page 14 C3 Submersible Fluorometer 3 3 Calibration Procedure 3 3 1 998 2300 Direct Concentration Calibration Following steps 1 5 below will ensure values are reported as blank subtracted Concentrations in units of choice See Appendix B for recommended practices Step 1 Blanking Place the C3 Submersible Fluorometer in a blank solution Note A blank solution is a solution without the fluorophore of interest i e deionized water artificial water or filtered water
14. e C3 Submersible Fluorometer and can be used in the future to establish communication with the instrument quickly 3 The Connect To screen will appear Choose the appropriate communications port in the Connect Using window Click OK 4 The Port Settings screen will appear Choose the following port settings Bits per second 9600 con Properties es Data bits 8 Por Satin Parity None Stop bits 1 fit prot O Flow control Hardware Click Apply and OK gagi dof Pag More m Stop b s i tt Fow conte Hawes Fasions Dalas OK Carmel Apok 5 Next the HyperTerminal window will appear You are now ready to connect the C3 Submersible Fluorometer to a power source Data will be displayed at set intervals in the HyperTerminal window after 100 seconds or at the specified log start time L Beppece riie Fe Ede Yee CH heas Help Di el ca a ata lime HhodasinePhcecvant Depth leap C 6 16 08 15 07 26 S0 fea 1279 70 OC 73 71 Wles 15 0 79 a1 s La Fl a 2a fz 6 16708 15 07 26 51 01 130 89 0w 33 72 bf lb Me To Bisel al ol 11 95 Ba 29 fA 6 16 08 15 07 28 51 09 139 95 0w 29 72 bf 16 08 15 07 79 a1 Lad T HW 279 f 6 16 08 15 07 30 51 23 131 18 68 33 72 998 2300 Rev N Page 29 C3 Submersible Fluorometer Most applications will integrate streaming data Below are instructions on how to save streaming data on a PC To save data 1 On the HyperTerminal window toolbar choose Transfe
15. ed States who purchased equipment from one of our authorized distributors contact the distributor If you purchased directly contact us We will repair the instrument at no charge Customer pays for shipping duties and documentation to Turner Designs Turner Designs pays for return shipment custom duties taxes and fees are the responsibility of the customer 4 4 Out of Warranty Service Follow steps for Warranty Service as listed above If our Technical Support department can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs Inc 845 W Maude Ave Sunnyvale CA 94085 998 2300 Rev N Page 23 C3 Submersible Fluorometer 998 2300 Rev N Page 24 C3 Submersible Fluorometer Appendix A Specifications C3 Submersible Fluorometer Weight in Air 1 64 kg 3 6 Ibs Length 23 cm 9 1 in Minimum Sampling Interval Signal Output Digital ASCII or Analog Note analog requires accessory Voltage Output 14 8V nominal Protects against overcharge over discharge overheating Protection Features Rata short circuiting 998 2300 Rev N Page 25 C3 Submersible Fluorometer Appendix B Recommended Practices Identification sensors are labeled 1 2 or
16. ger Be C Paulie back Update Detakegger Tene fore Hest hear Ore PE Curent Progen C306 Detelegging a a Page 33 C3 Submersible Fluorometer 2 3 Anew window will open titled Device Configuration Utility 2 06 Under Device Type in the left panel highlight the CR1000 Select the correct com port then click Connect CRIM ged Of CR1000 h onder to configura ita CATON pear 12 Volts DC must Be suppiad to tha daideo on ta Foses In pon A nina pin cabh should also ba connected betesen one af your computers ASAS Pods ard he AS 232 pod on the daidoggar When these requirements hove bean met adoi the eepropriota serial pon in iha kit gand and pres the Commece Button Ta 1 es rnin En ey By eT 2 j Pata 2 4 A warning message will come up asking you to bypass checking for com port conflicts with the local server click Bypass The warning message window will close Within the remaining Device Configuration Utility 2 06 window click on the ComPorts Settings tab Fi Option Help Devio Typa DST jogga Conio Date Mondor Fie conio Sendi Setings Giir Taring EED O s conr settings TOP cs OP pre retort Services ataned ERIN Paktum Security TRINPE CRHN TO CREE Saree Security Tode 1 Saari ode 2 l CRIN Security Cece 3 CRITPS DRITE IRON Choi Fabii a Gryphon Heg Coni Fafa Encryption kag p am Ei
17. ion is complete select Save Calibration Data are now displayed as blank corrected relative fluorescence units Rev N Page 16 3 3 3 3 3 4 998 2300 C3 Submersible Fluorometer Raw Fluorescence Calibration In the Current Data screen select the calibrate button next to a specific channel At the bottom of the Calibration Screen select Click to use uncalibrated Raw Fluorescence Mode Data for that channel will be uncalibrated and reported as Relative Fluorescence Units RFU Depth Calibration Optional Accessory Note Make sure your instrument is calibrated for depth and there Is a value set even if you do not have a depth sensor Depth is displayed in meters only There are two calibration options for depth 1 The first uses only the atmospheric pressure as an offset This is suitable for any shallow application including most fresh water applications 2 The second also compensates for gravity variations with latitude as designated from the UNESCO Technical Papers in Marine Science 44 http unesdoc unesco org images 0005 000598 059832EB pdf This method assumes 0 C and 35PSU salinity Chere Calbratioi Lin Laie Degth uret Pressure 000 Fal Step 1 Press Sat Zero to set the Zara Reading P Ti omre Zs Step 2 iar peur Comperntion J himophiri asit ay ri T imapi sd gaby ofa Sten 3 When you are dana Wes Ee Caraion of prem Canca at j Cancel ay Gime bo stort bois
18. l need a serial port The only pins used on the DB9 serial connector are 2 3 and 5 G Ground 998 2300 Rev N Page 32 C3 Submersible Fluorometer 2 Configuring the RS 232 port on the CR1000 2 1 After the CR1000 is powered and wired to your PC as described in section 1 connect to the CR1000 by clicking on Connect in the upper left corner of the PC200W Datalogger Support Software Rik Dattogge Meteo Tools Hel Vicon SS BA pon Py CicbProgum Pdonitor Data Colect Cate Datalogge Infeemation Duta 3 par Hare OF Oe Daiga Typs Ree Direct Connect Connection COR Fert CORNI atalogger Settings Brud Arin Hi Pain dirn 72D Temi Code 0 Extra Bagge Tire oh Dots Pe Paths Taua Ded Pee A Sa ca Public CCa phe Bech PC 008 0 FO Pub lic chat CE CC pn er PAC RO 06 chat Datalegger Re C Pause Chock Upd en iene SHH oat Curent Prog ch Ch Detulogging wR 2 2 Double click on the Device Configuration Utility icon in the toolbar of PC200W Fl Datlojgge Meteork Tools Help i CleckProgmm Monitor Data Colect ata Datalogge Infeemation Cut oj par Hema CFO Contain eer Typ CF Dri Coffees Connection COR Port COMMIS Gatalogger Settings Baud Arie Sees P n Address 72D aii Cade 0 Exin Rap ene Tire M Dots Pie Pathe Taa OC eel PCO AOA Sas chat Publis CCa pee inip IAR E Publie dat EE Ous al eee Pe aC RAN C6 cist 998 2300 Rev N Son ASA OH Bam amp O Chocks Datakeg
19. lti parameter systems such as CTD s and 3 Digital data that can be read and converted to analog data by the C Series Analog Adapter for output to an external datalogger or multimeter An 8 pin Impulse cable provides two end connections 1 a 9 pin RS232 serial port for connection to a PC or laptop computer and 2 a 12V port for supplying power to the unit C3 Submersible Fluorometer bulkhead and serial port connectors Sub D Connector Wire Guide C3 Function Corresponding Sub D Connector Pin V BATT Power Connector Cable Center Pin 3 Red GND PINS o oo B D 3 6 Brown DTR amp DSR PINS Le Power ground and V Batt are not common 998 2300 Rev N Page 27 C3 Submersible Fluorometer Appendix D Linear Range Quenching and Temperature Considerations The linear range is the concentration range in which the C3 sensor output Is directly proportional to the concentration of the signal The linear range begins with the smallest detectable concentration and spans to an upper limit concentration that is dependent upon the properties of the material filters used and path length A non linear relationship is seen at very high concentrations where the signal does not increase at a constant rate in comparison to the change in concentration see figure below At even higher concentrations the signal will decrease even though the sample concentrations are continuing to increase This effect is k
20. mory on your computer If you have not done a Disk Cleanup for awhile you may want to try and see if that clears up the problem and allows you to download files Disk Cleanup can be found in the following location on most Windows versions Start Menu gt All Programs gt Accessories gt System Tools gt Disk Cleanup Once the initial scan of the computer is complete customers should select all the check boxes and select OK to perform the cleanup 998 2300 Rev N Page 21 C3 Submersible Fluorometer 4 Maintenance and Warranty 4 1 Maintenance 4 1 1 Rinsing The C3 Submersible Fluorometer should be rinsed or soaked in freshwater following each deployment 4 1 2 Care for the bulkhead connector Install the 8 pin female end plug to the bulkhead between uses A light coat of Silicone spray should be used on the rubber of the male pins of the bulkhead to aid in sealing The manufacturer recommends 3M Silicone Lubricant Spray or Loctite 8021 spray Note You should avoid using silicone grease Do NOT use WD 40 it will destroy the connectors 4 2 Warranty Terms Turner Designs warrants the C3 and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions e Turner Designs is not responsible for replacing parts damaged by accident or neglect Your instrument must be installed according to inst
21. ne two or three optical sensors ranging in wavelengths from deep ultraviolet to infrared Each optical sensor is designed with fixed excitation and emission filters The C3 Submersible Fluorometer comes with a factory installed temperature probe and can be configured with a pressure sensor for depth measurements up to 600 meters and or a wiper to minimize biofouling The C3 Submersible Fluorometer Windows based user interface allows for easy calibration of each sensor digital data reporting data logging and digital or analog export of data 1 2 Important Note Regarding Use of Fluorescein Optics For C3 s manufactured prior to 2 15 2011 the Fluorescein sensor should not be enabled for simultaneous data collection with Chlorophyll and or Rhodamine sensors or it will produce unreliable results For C3 s manufactured after 2 15 2011 this interference issue was corrected If you would like to send your C3 back to Turner Designs to resolve this interference issue please contact sales turnerdesigns com 998 2300 Rev N Page 4 C3 Submersible Fluorometer 2 Inspection and Setup 2 1 Instrument Checklist The C3 Submersible Fluorometer package P N 2300 000 includes C3 Submersible Fluorometer Factory installed temperature sensor Antifouling Copper Tape Interface cable for communicating with your PC and supplying power to the instrument Integration Interface Adapter Cable see Appendix F and G for more informa
22. nown as signal quenching Linearity can be checked by diluting a sample 1 1 or some other convenient ratio If the sample is still in the linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the sample is beyond the linear range Fluorometer Response Curve Sample Quenching Region Graph showing Linear and Quenching Regions of the sample s response Sample ___Linear Region Temperature Considerations Fluorescence is temperature sensitive As the temperature of the sample increases the fluorescence decreases For greatest accuracy record the sample temperature and correct the sensor output for changes in temperature For further information on how temperature light water quality and the physiological state of the algal cells can all affect the measurement of chlorophyll please refer to the application section of Turner Designs website 998 2300 Rev N Page 28 C3 Submersible Fluorometer Appendix E How to set up HyperTerminal for Digital ASCII Data Capture 1 Ensure C Soft program is not running in the background On an MS Windows computer open the HyperTerminal program Start All Programs Accessories Communications HyperTerminal 2 The Connection Description screen will appear Name the connection description This file will save the communication parameters for th
23. nt Note As soon as C Soft is used to communicate with the instrument users will need to re set the parameter settings in the Log Setup section 3 4 and again enable datalog to put the instrument into the Data Output Ready State When power is supplied to the instrument while it is in the Data Output Ready State it will begin streaming data after a 100 second delay If power is cycled i e disconnected and reconnected please allow 100 seconds for the data stream to begin Note Check the optical face when power is supplied to make sure the sensors light The sensor will turn on then off and then turn back on in 100 seconds If this sequence does not happen cycle power 3 6 Downloading and File Management Users can download new and existing files saved in the C3 Submersible Fluorometer memory Select Download Data Note This step may take up to 10 minutes depending on file size When download has completed choose the files to be saved by clicking the Save box Users will be prompted to choose a folder location where data will be saved Files will be saved in csv format Select Clear Datalog to erase existing data from memory Users are able to graphically view downloaded csv data files using C Soft software if desired Select Gles to save 998 2300 Rev N Page 20 C3 Submersible Fluorometer Note If you have problems downloading data in C Soft you may have limited space in the cache me
24. ometer in order to log data capture real time data output and or integrate the C3 with other instrumentation see Appendix F and G for more information about integration There are five necessary steps to ensure the instrument is properly set up for data output 1 Set the instrument s clock to PC or local time in the Settings Tab 2 Select the sensors you expect to use during sampling 3 Calibrate selected sensors for either Direct Concentration Mode or Raw Fluorescence Units Blanked RFUB Mode see section 3 3 4 Set the start time sampling and wiping intervals in the Log Setup Tab Note Wiping interval is optional and depends on the sampling interval see section 3 4 5 Click the Enable Datalog button followed by the Yes button to enable the Data Output Ready State 998 2300 Rev N Page 19 C3 Submersible Fluorometer Note Use the Battery Life Calculator available on the Turner Designs website C3 product page to calculate a conservative estimation of how many days you can expect to log data Factors such as battery charge and water temperature will affect logging days A few seconds after the Yes button has been clicked to enable the Data Output Ready State the C Soft program will automatically shut down and users must disconnect power and serial connections The instrument is now in the Data Output Ready State and will remain in that state so long as C Soft is not used to communicate with the instrume
25. ple measurement and minimizes effects from ambient light Shade Cap Weight P N 2300 510 C ray Towed Deployment Body P N 2300 750 Extender Cables 10meters P N 105 2595 25 meters P N 105 2596 50 meters P N 105 2597 Custom lengths available upon request Booster Kit P N 2200 900 Note Required for cable lengths greater than 10 meters 24 Pigtail Interface Cable with Locking Sleeve P N 2200 170 Solid Secondary Standard Cap P N 2300 905 Solid Secondary Standard SSS for in vivo Chlorophyll Phycocyanin Phycoerythrin Rhodamine and Fluorescein P N 2300 901 See Accessory Instructions on the USB Flash Drive for more information 998 2300 Rev N Page 6 C3 Submersible Fluorometer Solid Secondary Standard SSS for UV Sensors CDOM FDOM Optical Brighteners Refined Fuels and Crude Oil P N 2300 902 See Accessory Instructions on the USB Flash Drive for more information Note Solid Secondary Standard not available for turbidity Analog Output Adapter P N 2300 480 See Accessory Instructions on the USB Flash Drive for more information Battery Bracket z High Capacity Submersible Battery Submersible Battery with Charger and Cable Shade Cap Booster Kit Booster 1 Booster 7 Air Purge Slots Solid Secondary Standard Flow Cap solid Secondary Standard Cap C ray Towed Deployment Body 998 2300 Rev N Page 7 C3 Submersible Fluorometer 2 3 Identification Sensors a
26. ppropriate COM port from the pull down COM menu Plug the 12V power supply into a power source 6 Wait for initialization process to complete D ieman i Initializing Unit Progra 998 2300 Rev N Page 10 C3 Submersible Fluorometer The connection icon located on the upper right of the screen will turn green if connected Note If the connection icon does not turn green disconnect and reconnect power Fim Settings COM Port Help 3 2 Software Operation 3 2 1 Settings Screen The Settings Screen allows users to set the time and date identify each optical sensor update serial numbers and enable disable sensors 998 2300 Users can manually set the date and time or choose to synchronize the C3 Submersible Fluorometer to the PC date and time Each channel 1 3 in the Settings Screen has a location associated with it on the C3 Submersible Fluorometer that is numerically labeled 1 3 respectively Channels 4 6 are designated for the C6 Multi Sensor Platform and are disabled for the C3 Click on the box adjacent to the channel to enable channel operation If there is no sensor installed users will be unable to select that channel Channels can be renamed using the dropdown selections or by manually entering a name The serial number boxes are designed for the C6 Multi Sensor Platform There is no serial number associated with the sensors in the C3 Settings will automatically be saved after exiting the Se
27. r and then Capture Text File Edit View Call Transfer Help l Z at Sdri k t wl Sl al Receive File Capture Text 2 When you click on Capture Text a window will appear asking you to name and save the txt file to the location of interest 3 Once you are finished you will need to return to the HyperTerminal window toolbar choose Transfer and then Capture Text and select Stop This will stop data capture to the txt file File Edit View Call Transfer Help Dice s 3 x sili apture Text Send Text File Capture to Printer 998 2300 Rev N Page 30 C3 Submersible Fluorometer Appendix F Integrator Firmware Turner Designs offers Integrator Firmware This firmware is designed for use by customers that are integrating their C3 with an external datalogger CTD or similar system The Integrator Firmware is different from the standard C3 firmware and has some features that users need to consider before installing 1 Internal datalogging is NOT possible with this version of firmware The Integrator Firmware is intended for data output only 2 The data output rate defined by this Integrator Firmware is 1 second and cannot be changed to reflect longer or shorter data output rates 3 Once power is applied there is a 15 second delay before data output starts at a 1 second interval 4 Ifthe wiper is enabled it will rotate the set number of rotations upon powering the instrument and every 5 min
28. re marked with numbers 1 2 or 3 located adjacent to the optics on the face of the C3 Submersible Fluorometer These numbers correspond to channels 1 2 and 3 in C Soft C3 software Note Channel names can be modified using C Soft Light emitting diode color table Application Light Source Color In Vivo Chlorophyll Blue Turbidity Infra Red No Color Phycocyanin Yellow Orange Phycoerythrin Fluorescein ee See important note on page 5 Po Green Green pC PTSA PTSA Ultra Violet No Color Tryptophan Deep Ultra Violet No Color Do not look directly at the optics Ultraviolet light can be damaging to the eyes 998 2300 Rev N Page 8 C3 Submersible Fluorometer 3 C Soft Windows User Interface The C Soft Windows based user interface allows for intuitive calibration data logging set up and download functions 3 1 Software Installation and PC Requirements 3 1 1 Minimum PC Requirements 4 4 133MHz microprocessor 600 X 800 VGA resolution Microsoft Windows 98 32 MB RAM 3 1 2 Software Installation 1 2 Exit all Windows programs Insert the USB Flash Drive also located on the Turner Designs Software Firmware webpage under Customer Care Open the C Soft software folder and double click on the setup icon The software will automatically be installed in the PC s C Program Files C Soft folder unless user selects alternate location After restarting the PC the C
29. ructions in the User s Manual Damage from corrosion is not covered Damage caused by customer modification of the instrument is not covered e This warranty covers only Turner Designs products and is not extended to equipment used with our products We are not responsible for incidental or consequential damages except in those states where this limitation is not allowed This warranty gives you specific legal rights and you may have other rights which vary from state to state e Damage incurred in shipping is not covered 998 2300 Rev N Page 22 C3 Submersible Fluorometer 4 3 Warranty Service To obtain service during the warranty period the owner shall take the following steps 1 Write email or call the Turner Designs Technical Support department and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com Carry out any adjustments or tests as suggested by the Technical Support Department If proper performance is not obtained you will be issued a Return Materials Authorization number RMA to reference Package the unit write the RMA number on the outside of the shipping carton and ship the instrument prepaid to Turner Designs If the failure is covered under the warranty terms the instrument will be repaired and returned free of charge for all customers in the contiguous continental United States For customers outside of the contiguous continental Unit
30. s and make sure to set C Soft to that specified COM designation when trying to connect via USB Adapter Cable connection 10 If the USB Serial Port s COM designation is greater than 6 right click on the USB Serial Port and click properties which will open up the properties menu From that menu click on the Port Setting tab and then click Advanced which will open the advanced settings menu for that USB Serial Port Choose any COM port 1 6 from the COM port pull down menu and click OK Note Ideally you want to start with any port that is not in use if all are in use then choose any port 1 6 Click OK to the warning box if it appears and Click OK to close any other open menus Your installation is complete Make sure to set C Soft to that specified COM designation when trying to connect via USB Adapter Cable connection 3 1 4 PC Software Interface Note It is important that the steps be followed INORDER Any variance from the procedure may result in the connection NOT being established 1 Plug either the USB or serial connector of the adapter cable into your computer Note Either connection can be used for communicating and configuring the instrument 2 Plug the interface cable onto the 8 pin bulkhead connector of the instrument 3 Plug the interface cable s serial plug into the adapter cable s male serial plug 4 Begin C Soft by double clicking on the C Soft icon and choose the a
31. tion about integration USB Flash Drive including C Soft software user s manual cable guide and quick start guide 12V Power Supply _ 1 we Antifouling Copper Tape i ene Integration Interface Adapter Cable 998 2300 Rev N Page 5 C3 Submersible Fluorometer 2 2 Optional Accessories Pressure sensor P N 2300 360 Factory installed Note Can only be installed during the manufacturing of the unit C3 Mechanical Wiper P N 2300 450 Factory installed Note Can only be installed during the manufacturing of the unit See Accessory Instructions on the USB Flash Drive for more information Replacement Wiper Brushes P N 2300 460 Replacement Wiper Spacers P N 2300 462 Antifouling Copper Tape P N 2300 506 Rechargeable Submersible Battery Pack including charger P N 2200 601 See Accessory Instructions on the USB Flash Drive for more information Battery Bracket P N 2300 603 Note Required for use with Battery Pack and Analog Output Adaptor See Accessory Instructions on the USB Flash Drive for more information High Capacity Submersible Battery with Charger and Cable P N 3200 600 See Accessory Instructions on the USB Flash Drive for more information Flow Cap P N 2300 700 See Accessory Instructions on the USB Flash Drive for more information Shade Cap P N 2300 500 or C ray Shade Cap P N 2300 502 Note We recommend use of the shade cap as it provides a fixed distance for calibration and sam
32. ttings screen Rev N Page 11 C3 Submersible Fluorometer File Teething OOH Peat bain Conrancted feibings Current kta 20 Log Gebep Deeded Duta aired Set tires mhis Hara Serial air T han 1 E Phirisa El LUE Gurren Tires in Dti ee Chiraphed a iml ESINE ne Te Cheat 4 Preia p pi fA Lz a HEET Set Ling To PS Drs B Tons E RLA LYENI ca wh fe Mupraasihy Dat Link Caie ih Tira Ping Corea Sat mi at 3 2 2 Current Data and Calibration Screen 998 2300 The Current Data Screen allows users to view real time data acquisition in both tabular and waveform formats as well as conduct calibrations for each channel and view calibration information Current Data 4 Channel names are displayed but cannot be changed in the Current Data screen Real time values are displayed next to each channel and in the adjacent Data Table A calibration button is available for each channel and the depth sensor Real time data can be saved by selecting the Start Data Capture button Users will be prompted to enter a file name and file location Data files will be saved as csv format Tabular Screen Real time data including date time units and temperature are continuously scrolling on the tabular screen for enabled channels Channel names will appear in the associated column headings If units are not selected during calibration a relative
33. ture Integrator Firmware Campbell Scientific CR1000 Datalogger Integration Qm7MOUQMdW D o O1 C3 Submersible Fluorometer WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT WEEE DIRECTIVE Turner Designs is in the business of designing and selling products that benefit the well being of our environment Accordingly we are concerned with preserving the Surroundings wherever our instruments are used and happy to work with customers by complying with the WEEE Directive to reduce the environmental impact resulting from the use of our products WEEE Return Process To arrange the return of an end of life product proceed as follows lf you purchased your instrument through a Turner Designs Distributor please contact your local representative They will instruct you where to return the end of life product If you purchased your instrument directly from Turner Designs please contact Turner Designs Customer Service By Phone 1 408 212 4041 or Toll Free 877 316 8049 By Email Customer Service at support turnerdesigns com Turner Designs will provide a WEEE RMA Number a Shipping Account Number and a Ship to Address Package and ship the product back to Turner Designs The product will be dealt with per Turner Designs end of life recycling program in an environmentally friendly way C3 Submersible Fluorometer 1 Introduction 1 1 The C3 Submersible Fluorometer is manufactured according to users optical specifications with o
34. utes as long as power is continuously supplied 5 Sleep mode is disabled therefore as long as power is continuously supplied the instrument will continue to stream data at a 1 second interval Download Firmware on the Turner Designs website http www turnerdesigns com customer care software a firmware 998 2300 Rev N Page 31 C3 Submersible Fluorometer Appendix G Campbell Scientific CR1000 Datalogger Integration This document assumes you have experience connecting to your CR1000 The procedure of interfacing the C3 Submersible Sensor is described in four sections Wiring the CR1000 to aPC Configuring the RS 232 port on the CR1000 Connecting the C3 to the CR1000 Loading the CRBasic program C3_C6 Datalogging v2 CR1 onto the CR1000 This file can be found on our website at the following address http www turnerdesigns com t2 sw C3 C6 Datalogging CR1 zip Note Sections 2 and 4 require Campbell Scientific PC200W software Campbell Scientific PC200W software can be found at the following web address http Awww campbellsci com downloads sb PC200W amp c 9999 1 Wiring the CR1000 to a PC Typical communication between a PC and a CR1000 uses the RS 232 port on the CR1000 However the C3 requires RS 232 logic levels and therefore it will need to occupy the RS 232 port As a result the PC will communicate with the CR1000 using COM1 on the lowest terminal block In order for the PC and the CR1000 to communicate your PC wil
35. v N Page 35 C3 Submersible Fluorometer 4 Loading the CRBasic program onto the CR1000 using PC200W software 4 1 After the CR1000 is powered and wired to your PC as described in section 1 connect to the CR1000 by clicking on Connect in the upper left corner of the PC200W Datalogger Support Software Fl Catsiogge Meieri Toos Hep Yom SS OB pan o Cicbtrmgum fonto Daa Collect sta Datalogge Infeemation Chocks Distal oy per Haree CF Ceatadony ger Type CR i ieman as Be Dritti Cones Connection COR Fort COMMIS atalogger Settings area Catalogger Tene Zone Hm Sanity Code 0 ef Chih Theser Ne E Erin Aap eee Tira Oh C Pause Clock Upsi Pathe Oataieeqqer Peogqram CAL bee Po eas coat Public CCa pa Beef 2 D080 R EN Publie chat Lurent Prr ZE CO Care pi ere Ri 06 cht ci CE Detsloggng xR 4 2 After connecting the GUI will display a Connection Time in the lower right corner Click on the Send Program button The delivery of the CR1 file will cause all data on the CR1000 to be deleted The GUI will prompt you if this is OK Click Yes Navigate to the directory where you have the C3_C6_ Datalogging_v2 CR1 file highlight it and then click Open Fle Dutalogger Meteor Took ais Woru R A ao PETA o Csi Propam Morstor Data Collect Bute Dita iegger Aur alia Thks CATES Dalah gegen Mena CHIDO resid Drinbigjer Toge CAE Cla og ir wash nancial Fp orted Ss ca Flere a Cee ac
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