Home

Chemical Hygiene Plan and Hazardous Materials Safety Manual

1. l f E i DUE UNIVERSITY CHEMICAL HYGIENE PLAN AND HAZARDOUS MATERIALS SAFETY MANUAL Adopted February 2014 Chemistry modifications Fall 2014 The official version of this document will only be maintained online PURDUE UNIVERSITY Chemical Hygiene Plan and Hazardous Materials Safety Manual Laboratory Specific Plan This is the Chemical Hygiene Plan specific to the following areas Building s Brown Hall Room Number s 4144 and 4144A Principal Investigator Supervisor Dr Shelley Claridge Department Department of Chemistry Revised Must be reviewed at least annually 20141009 Important Telephone Numbers 1 911 for All Emergencies 2 765 49 48221 Purdue Police Department Non Emergency Line 3 765 49 46919 Purdue Fire Department Non Emergency Line 4 765 49 46371 Purdue REM Do Not Use for an Emergency All laboratory chemical use areas must maintain a work area specific Chemical Hygiene Plan which conforms to the requirements of the OSHA Occupational Exposure to Hazardous Chemicals in Laboratories 29 CFR 1910 1450 Purdue University laboratories may use this document as a starting point for creating their work area specific Chemical Hygiene Plan Minimally this cover page is to be edited for work area specificity non West Lafayette laboratories are to place their own emergency fire and police telephone numbers in the space above and the Purdue Chemical Hygiene Plan Awareness C
2. The Purdue University Institutional Biosafety Committee IBC is the campus based committee that has the responsibility for reviewing and approving all proposals activities and experiments involving an organism or product of an organism that presents a risk to humans plants animals or the environment The PI must submit to the IBC an application to use rDNA synthetic nucleic acids potential pathogens human tissue fluids and or cell lines in their research The IBC review is conducted in accordance with the guidance and requirements of National Institutes of Health the Centers for Disease Control and Purdue University policies and the Biosafety Manual All Pls have an obligation to be closely familiar with EHS guidelines applicable to their work and to adhere to them More detail regarding the IBC process can be found on the Purdue Office of the Vice President for Research webpage http www purdue edu research vpr rschadmin rschoversight rdna forms php 3 4 Radioactive Material Hazards The Purdue University Radiation Safety Committee RSC is the campus based committee that has the responsibility for reviewing and approving all proposals activities and experiments involving radioactive material and radiation producing devices The PI must submit to the RSC through REM an application to use radioactive material or radiation producing devices Use of radioactive materials at Purdue University is authorized under a license issued by the
3. Chapter 14 Section 4 Potential Hazards Base baths are flammable and corrosive They may be harmful if inhaled ingested or absorbed through the skin Inhalation may cause irritation to the respiratory tract with burning pain in the nose and throat coughing wheezing shortness of breath and pulmonary edema It is destructive to the tissue of the mucous membranes and upper respiratory tract Inhalation of solvent vapors may cause chronic toxic effects in the liver or kidney Contact with skin causes burns and irritation Prolonged or repeated skin exposure may cause skin defatting or dermatitis Eye contact causes burns irritation and may cause blindness Ingestion may cause permanent damage to the digestive tract Flash fires may occur in the presence of ignition sources Chapter 15 Section 5 Personal Protective Equipment PPE Respirator Protection If base baths are being used outside of a chemical fume hood respiratory protection may be required If this activity is absolutely necessary contact REM so a respiratory protection analysis can be performed Respirators should be used under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted e When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded Tab 2 Lab Specific Protocols Requirements Rules e Regulations require the use of a respi
4. Biological safety cabinets are not designed to be used with chemical applications so the use of chemicals should be kept to a minimum Applications that involve the use of chemicals should be conducted in chemical fume hoods Figure 4 5 Biological Safety Cabinet 4 2 5 Safety Showers and Eyewash Stations All laboratories using hazardous chemicals must have immediate access to safety showers and eye wash stations Safety showers must have a minimum clearance of 24 inches from the centerline of the spray pattern in all directions at all times Identify the safety station with a highly visible sign and maintain an unobstructed path to it All lab personnel must be aware of the location and know how to properly use the safety shower and eyewash Stations If lab personnel are exposed to a hazardous chemical they should dial 911 or someone else in the lab that is not exposed should dial 911 and use the safety shower and or eye wash unit for 15 minutes or until emergency response have personnel arrive and begin treatment If an uninjured individual is present this person should assist with the decontamination of the affected individual All eyewash stations must be flushed by laboratory personnel on a weekly basis to ensure proper working order This will keep the system free of sediment and prevent bacterial growth from reducing performance REM performs annual inspections of all campus safety shower and eyewash stations This inspection evaluate
5. Examples of embryotoxins include thalidomide and certain antibiotics such as tetracycline Women of childbearing potential should note that embryotoxins have the greatest impact during the first trimester of pregnancy Because a woman often does not know that she is pregnant during this period of high susceptibility special caution is advised when working with all chemicals especially those rapidly absorbed through the skin e g formamide 93 The official version of this document will only be maintained online B 2 5 3 Appendix B OSHA Hazard Class Definitions Substances with a High Acute Toxicity Substances that have a high degree of acute toxicity are materials that may be fatal or cause damage to target organs as the result of a single exposure or exposures of short duration Acute toxins are quantified by a substance s lethal dose 50 LDso or lethal concentration 50 LCso which is the lethal dose of a compound to 50 of a laboratory tested animal population e g rats rabbits over a specified time period High acute toxicity includes any chemical that falls within any of the following OSHA defined categories A chemical with a median lethal dose LD50 of 50 mg or less per kg of body weight when administered orally to certain test populations A chemical with an LD50 of 200 mg less per kg of body weight when administered by continuous contact for 24 hours to certain test populations A chemical with a medi
6. F Relative Vapor Density 0 97 air 1 Section 4 Potential Hazards Liquid nitrogen is a cryogenic liquid that may cause severe frostbite or eye damage upon contact Extremely cold liquid and gas under pressure Expands by a factor of 700 upon vaporization Can cause rapid suffocation due to displacement of oxygen Avoid breathing gas Substances may become brittle upon contact and shatter May cause an explosion of a sealed container Symptoms to exposure included frostbite dizziness salivation nausea vomiting or loss of mobility and or consciousness Section 5 Personal Protective Equipment PPE For quantities less than 1 L safety glasses cryo gloves long pants and close toe shoes are adequate Figure 1 For intermediate quantities 1 L 10 L also use splash goggles or face shield and cryogenic gloves Figure 2 For quantities greater than 10 L or filling a large secondary Dewar a cryogenic apron should be used in addition to the general use intermediate requirements Figure 3 Tab 2 Lab Specific Protocols Requirements Rules Figure 3 Large quantity PPE requirements Section 6 Engineering Controls Liquid nitrogen must only be used in a well ventilated area or in a properly functioning chemical fume hood whenever possible Liquid nitrogen should never be used in a poorly ventilated enclosed area where oxygen displacement is a possibility Section 7 Liquid Nitrogen Containers Vacuum Insulated
7. me m m n o a lt 2 eed et a _ _ __ _ _ _ Figure 4 1 Chemical Fume Hood Sash Approved Working Height Chemical fume hoods must be equipped with a continuous reading monitoring device to indicate adequacy of flow All lab employees must know how to read and interpret this gauge and check that the chemical fume hood is operating properly before using hazardous chemicals in the fume hood There are many different types of chemical fume hoods on campus so it is important that the lab employee understands the specific functions of each chemical fume hood used 20 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls Only apparatus and chemicals essential to the specific procedure or process should be placed in the chemical fume hood Extraneous materials from previous experiments should be removed and stored in a safe location outside the chemical fume hood Chemical fume hoods used for experimental work should not be used for chemical or material storage Chemical fume hoods used for chemical storage should be dedicated to chemical storage No experimental work should be conducted in storage chemical fume hoods All chemical containers used in chemical fume hoods including secondary containers e g beakers flasks reaction vessels vials et
8. see section 7 7 of this chapter for information and guidance for acceptable sink disposal practices Hazardous wastes can be liquid solid gas or sludge They can be discarded chemicals or mixtures generated from research and teaching operations commercial products e g cleaning fluids or pesticides or by products of manufacturing processes All hazardous waste falls into one of the following categories e Characteristic Wastes includes wastes that are ignitable corrosive reactive or toxic D listed e Listed Wastes includes wastes from common manufacturing and industrial processes F listed wastes from specific industries K listed and wastes from commercial chemical products U and P listed e Universal Waste includes certain batteries primarily rechargeable batteries such lithium nickel cadmium nickel metal hydride and mercury oxide mercury containing equipment e g thermometers thermostats and certain lamps e g fluorescent bulbs Note alkaline batteries and incandescent bulbs are not considered Universal Wastes and can be legally disposed of as trash e Mixed Waste hazardous waste mixed with radioactive waste EPA regulated hazardous waste should not be mistaken for biological or radiological wastes A more detailed definition of hazardous waste including the D F P and U lists is provided in Appendices 1 and 2 of the Hazardous Waste Disposal Guidelines http www purdue edu rem home booklets hwdg
9. 5 7 4 of the CHP details the storage requirements for reactive chemicals B 1 6 Oxidizers An oxidizing solid liquid is a solid liquid which while in itself is not necessarily combustible may generally by yielding oxygen cause or contribute to the combustion of other material Hydrogen peroxide nitric acid and nitrate solutions are examples of oxidizing liquids commonly found in a laboratory Sodium nitrate Sodium perchlorate and Potassium permanganate are examples of oxidizing solids commonly found in a laboratory Chapter 5 7 7 of the CHP details oxidizer storage requirements B 1 7 Organic Peroxides An organic peroxide is an organic liquid or solid which contains the bivalent 0 0 structure and may be considered a derivative of hydrogen peroxide where one or both of the hydrogen atoms have been replaced by organic radicals The term also includes organic peroxide formulations mixtures Such substances and mixtures may e Be liable to explosive decomposition e Burn rapidly e Be sensitive to impact or friction or e React dangerously with other substances Chapter 5 7 7 of the CHP details organic peroxide storage requirements 90 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions B 1 8 Explosives An explosive substance or mixture is a solid or liquid substance or mixture of substances which is in itself capable by chemical reaction of producing gas at s
10. Chapter 6 Laboratory PPE Policy 6 4 3 Respiratory Protection The use of respirators in the laboratory setting is not typically necessary since all work involving hazardous materials must be conducted in a chemical fume hood whenever possible When ventilation is not adequate to provide protection against an inhalation hazard respiratory protective equipment may be necessary There is a variety of respiratory protective equipment available for use but no one device will provide protection against all possible hazards Respirator selection is based on the chemical and process hazard and the protection factors required Respirators are not to be used except in conjunction REM s Respiratory Protection Program This program includes a review of the process to ensure that proper equipment is selected for the job training of all respiratory protective equipment users concerning the methods for proper use and care of such equipment fitting of respirator users when required and medical surveillance of respirator users when required Contact REM at 765 49 46371 with questions about the Respiratory Protection Program or visit the REM webpage http www purdue edu rem home booklets RPP98 pdf 6 4 4 Eye and Face Protection Each affected employee must use appropriate eye and face protection equipment when exposed to hazards from chemical splash flying debris or other exposures that may occur in the laboratory Safety glasses must be worn
11. Containers Vacuum insulated containers are used for storing and dispensing liquid nitrogen They are either sealed capable of holding 20 psig 240 psig with pressure relief valve or ambient pressure covered loosely with a cap cork or stopper and are referred to as Dewars due werz There are two primary types of Dewars benchtop and large Dewars Benchtop Dewars Figure 4 are typically for small scale laboratory use and the lid is the only pressure relief device Large Dewars Figure 5 are typically used for storage of lab samples movement of samples between campus locations or to fill other secondary containers Large Dewars may have a secure seal and pressure relief vent Liquid nitrogen exposure is a risk when handling Dewars Therefore the PPE illustrated in Figure 2 should be worn when working with a Dewar Cryogenic Tubes Tab 2 Lab Specific Protocols Requirements Rules Cryogenic tubes are typically used for storage samples movement of samples between campus locations or for shipments off campus for collaborative research Figure 6 There is no pressure relief device on a cryogenic tube other than the lid Consequently cryogenic tubes can explode without warning Explosions are likely caused by trapped nitrogen expanding inside of the tube during the thawing process As the temperature increases the tube may become over pressurized and explode and may result in serious injuries Because of this risk the PPE illustra
12. PPE Policy See REM webpage for the location evaluation hazard assessment template http www purdue edu rem home files forms htm ppe 6 3 3 Job Title Evaluation Hazard Assessment Job title evaluation hazard assessments should be conducted for specific positions These types of hazard assessments should be written in a comprehensive manner that includes the majority of hazards that a specific job position e g Animal Care Technician routinely encounters during the normal course of work This type of hazard assessment is commonly used for positions where the hazards encountered do not frequently change If the employee encounters a hazard that is not covered by the job title evaluation hazard assessment then it will be necessary to perform another type of hazard assessment such as the task evaluation hazard assessment that does address the specific hazards of that task The following describes the steps that should be taken to perform a task evaluation hazard assessment e Identify hazards that the position title may encounter while performing normal duties e List each task where hazard is present e Determine PPE requirements for each task e List other control measures required See the REM webpage for the job title evaluation hazard assessment template http www purdue edu rem home files forms htm ppe 6 4 Minimum PPE Requirements for Laboratories This section details the minimum PPE requirements for all laboratories using ha
13. Po RED OdUCHUVE TOXIN a E A 93 B 2 5 3 Substances with a High Acute Toxicity ssseessseneseensseessseesssrrssserssrersseersseee 94 Appendix C Peroxide Forming Chemicalls cccccsccscsscccccscsccccccscsccccccscsceccccscscescccecscecess 95 Tab 1 Specific Standard Operating Procedures ccscscsscscscccsccscsccccccscnceccecscnceccccecscecess 98 Tab 2 Lab Specific Protocols Requirements Rules ccscscscsccscsccccccscsceccccscsceccccecscecess 99 Tab 3 Lab Specific Hazard Assessments csceccccscsceccccscsceccccscnceccccscnceccccecsceceececscesescess 132 The official version of this document will only be maintained online ANSI ASTM CFR CHO CHP CLSC DOT EHS EPA GFCI GHS HBr HF HEPA HPLC IBC IDEM ISP LC LC50 LD50 LEL LSC MSDS NFPA OSHA PCB PHS PI PPE RCRA rDNA REM RSC SAA SDS SOP UEL CHP Document Acronyms List CHP Document Acronyms List American National Standards Institute American Society of Testing and Materials Code of Federal Regulations Chemical Hygiene Officer Chemical Hygiene Plan Chemical and Laboratory Safety Committee Department of Transportation Environmental Health and Safety Environmental Protection Agency Ground Fault Circuit Interrupter Globally Harmonized System of Classification and Labeling of Chemicals Hydrogen Bromide Hydrofluoric Acid High Efficiency Particulate Air High Performance Liquid Chromatography Institutional Biosafe
14. Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Label Waste Make sure the waste container s is properly labeled label should indicate all of the contents of the container REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area flammable cabinet is recommended Dispose of Waste Before submitting compressed gas waste to REM ensure that the cylinder cannot be returned to the manufacturer or distributor Many gas vendors charge demurrage for gas storage Most lecture bottles cannot be returned to the manufacturer and must be treated as waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Section 12 Safety Data Sheet SDS Tab 2 Lab Specific Protocols Requirements Rules A current copy of the SDS for the specific compressed gas being used must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs c
15. US Nuclear Regulatory Commission or a registration with the Indiana State Department of Health and all work must comply with applicable regulations The policies and procedures for handling radioactive materials are contained in the Purdue University Radiation Safety Manual http www purdue edu rem home booklets radman pdf 3 5 Laser Hazards The Purdue University Laser Safety Committee LSC is the campus based committee that has the responsibility for reviewing and approving all proposals activities and experiments involving laser radiation devices Pls must submit to the LSC through REM an application to use Class 3B and Class 4 lasers or laser devices The use of lasers is subject to OSHA regulations and utilizes current ANSI standards to develop guidance The policies and procedures for handling lasers are contained in the Purdue University Laser Safety Guidelines http www purdue edu rem home booklets laserguide pdf 18 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls Chapter 4 Laboratory Safety Controls Laboratory safety controls include engineering controls administrative controls and PPE Elements of these three categories should be used in a layered approach to minimize employee exposure to hazardous chemicals The hierarchy of controls prioritizes hazard mitigation strategies on the premise that the best way to control a hazard is to systematically eliminate it f
16. a laboratory scale Multiple chemical procedures or chemicals are used The procedures involved are not part of a production process nor in any way simulate a production process and 2 The official version of this document will only be maintained online Chapter 1 Introduction 4 Protective laboratory practices and equipment are made available and in common use to minimize the potential for employee exposure to hazardous chemicals The CHP was prepared in accordance with the requirements of the Occupational Safety and Health Administration OSHA Occupational Exposure to Hazardous Chemicals in Laboratories Standard Lab Standard found in 29 CFR 1910 1450 and is based on best practices identified in among other sources the Global Harmonized System of Classification and Labeling of Chemicals Prudent Practices for Handling Hazardous Chemicals in Laboratories published by the National Research Council the American Chemistry Society Task Force on Laboratory Chemical and Waste Management s Laboratory Waste Management A Guidebook the Princeton University Laboratory Safety Manual and the University of California Los Angeles Chemical Hygiene Plan 1 3 CHP Use Instructions The information presented in the CHP represents best practices and provides a broad overview of the information necessary for the safe operation of laboratories that utilize hazardous chemicals It is not intended to be all inclusive D
17. a shut off device installed e Ensure that all water connections on the condenser are clamped securely e Inspect all glassware for defects before setting them up in the experiment e Keep all air and water sensitive drying agents under inert atmosphere Make consistent efforts to not store or use other flammable or hazardous chemicals inside the fume hood where distillation is taking place 48 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 12 5 Laboratory Glassware Broken laboratory glassware is dangerous Glassware related injuries ranging from small cuts to multiple stitches and eye damage are common to lab workers In order to reduce the risk of accidents the following guidelines should be followed Temperature changes can shatter any laboratory glassware Never flash cool glassware with cold water especially after autoclaving or exposure to any high temperatures Only round bottomed or thick walled e g Pyrex evacuated reaction vessels specifically designed for operations at reduced pressure should be used Inspect glassware for any small imperfections before using Sometimes a hairline crack may be present Tap the glassware with a pen and listen to the tone to tell if there is a defect A ringing tone indicates the glassware is fine while a dull thud indicates there is a flaw present Don t keep cracked glassware If the bottom of a graduated cylind
18. at all times by all individuals that are occupying the laboratory area Splash proof safety goggles and or a face shield may be more appropriate depending on the type of work being performed e g transferring hazardous liquids outside of a chemical fume hood or glove box All eye protection equipment must be American National Standards Institute ANSI approved and appropriate for the work being done Eye and face protection may not be required in the lab if the employee is sitting at a workstation or desk that is away from chemical processes e g working at a desktop computer having a lab meeting at a table away from hazardous operations 6 4 5 Hand Protection Each affected employee must wear appropriate hand protection when the hands may be exposed to skin contact of hazardous chemicals cuts abrasions punctures or harmful temperature extremes Chemical resistant gloves must be worn while handling any 65 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy hazardous chemical container regardless of whether the container is open or closed it should be assumed that all chemical containers are contaminated When selecting appropriate gloves it is important to evaluate the effectiveness of the glove type to the specific hazardous chemical being handled Some gloves are more suitable for certain hazardous chemicals than others The SDS for the specific chemical being handled a
19. bath should be placed in a tray capable of containing the full bath volume in the event that the bath container fails e Keep container upright amp closed in a dry and well ventilated place e Base baths solutions must be stored in appropriate containers such as a heavy duty HDPE Nalgene container Do not store base bath solutions in metal containers Do not store base bath solutions Rubbermaid containers or other non chemical approved storage containers e Avoid contact with skin and eyes Avoid inhalation of vapor or mist e Keep away from incompatible materials such as acids and oxidizing materials Keep away from sources of ignition Avoid heat and shock or friction when handling e Containers should remain closed when not in use Chapter 19 Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surfa
20. corrosives oxidizers or organic peroxides at one time High hazard tasks can also include work with equipment that creates particularly hazardous conditions Examples include solvent distillation work with high pressure systems hydrogenation work with cryogenic chemicals such as liquid nitrogen etc 83 The official version of this document will only be maintained online Chapter 9 Training Laboratory Supervisors must ensure that all applicable personnel receive documented training on lab specific SOPs More information regarding SOPs can be found on the REM website http www purdue edu rem home files sop htm 9 4 Laboratory Chemical Safety Course CHM 605 The Purdue Department of Chemistry offers a graduate level zero credit fall semester course CHM 605 that covers the fundamentals of laboratory chemical safety practices CHM 605 includes a strong focus on learning how to find read interpret and use the information in safety data sheets chemical labels and other printed chemical safety information Other topics covered include fire protection prevention electrical safety laser and ionizing radiation safety machine safety pumps autoclaves centrifuges and non PPE safety equipment hoods extinguishers fire protection systems and building elements general ventilation showers eyewashes and an understanding of administrative controls engineering controls and how to select use maintain and decide to retire replac
21. discuss how to properly ship chemical off campus using a shipping company how to transport chemicals on campus using a Purdue owned vehicle and how to safely move chemicals by foot across campus 52 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 14 1 Shipping Hazardous Chemicals off Campus Shipping chemicals research samples or other similar materials off campus is potentially regulated by the Department of Transportation DOT and or other regulatory agencies Chemicals regulated for shipping require very specific types of packaging labeling and documentation and must be prepared by trained personnel REM makes the determination on whether a chemical is classified as hazardous for transportation purposes Unless the researcher is DOT trained they are not authorized to make this determination Shipments that are not prepared by trained personnel can result in delays loss of research samples and potential regulatory fines REM can provide assistance by either providing shipment services or if necessary training personnel on the proper shipping procedures More information about shipping chemicals can be found on the REM webpage http www purdue edu rem hmm shiphm htm As previously stated REM prepares all regulated chemicals for shipment according to DOT requirements However laboratory personnel prepare the inner container e g vial jar and provide it to REM f
22. e g HEPA filters need to be changed ensure the maintenance is performed by a qualified individual 30 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan e Use required PPE as instructed by the PPE Policy detailed in Chapter 6 5 2 Housekeeping Housekeeping is an important element to a laboratory safety program A clean well maintained lab improves safety by preventing accidents and can enhance the overall efficiency of the work being performed The following laboratory housekeeping guidelines should be followed e All doorways and hallways must be free of obstructions to allow clear visibility and exit The laboratory should be uncluttered without excessive storage of materials that could cause or support a fire e g paper cardboard flammable liquids etc e Fire protection sprinklers must be unobstructed a minimum of 18 inches of clearance is required below the sprinkler head If the laboratory does not have fire protection sprinklers there must be a minimum of 24 inches of clearance below the ceiling e Do not store items that block fire extinguishers or eyewash and safety shower stations e Do not store items in front of electrical boxes panels in the lab e A routine cleaning schedule should be established All work surfaces should be kept as clean as possible All potentially chemically contaminated work area surfaces e g chemical fume hood deck countertops shoul
23. edu rem home files contlabl htm 4 4 Personal Protective Equipment PPE Personal protective equipment PPE should be used to supplement engineering controls However PPE should never be used as a substitute for engineering controls when engineering controls are required PPE must be worn at all time in the laboratory when handling hazardous chemicals Proper PPE selection can be determined in the following ways e Ask the Laboratory Supervisor about proper PPE selection e Review the SOP and associated hazard assessment for the task to be performed e Review Section 8 Exposure Controls Personal Protection of the SDS for the chemical s being used This will provide basic information on the PPE recommended for use with the particular chemical The SDS addresses worst case conditions therefore all the equipment described may not always be necessary for a specific job In addition the SDS may not provide sufficient information concerning a specific respirator or type of glove appropriate for the chemical Additional PPE requirements are detailed in the Laboratory PPE Policy in Chapter 6 and Chapter 9 of the CHP 28 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan Chapter 5 Laboratory Management Plan An effective laboratory management plan is essential to operating a safe lab environment Requirements on topics such as lab housekeeping chemical inventories proper han
24. empty containers with a solvent capable of removing the original material e Collect the rinsate for disposal through REM e Identify triple rinsed dry odorless and empty containers by placing a Safe for Disposal label on the container Figure 7 5 Contact REM at 765 49 40121 to request a supply of these labels e Remove any cap that may cause the container to become pressurized when compacting e Arrange removal of these containers with the Building Services staff in your area or take these containers to the designated area beside the dumpster outside your building Figure 7 5 Safe for Disposal Label e If unable to remove residual hazardous materials from containers submit these to REM for pickup using the Hazardous Materials Pickup Request Form 74 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management 7 5 Waste Disposal Procedures REM provides pickup services for all chemical waste generated on the West Lafayette campus A Hazardous Materials Pickup Request Form must be completed and submitted by the generator of the waste to initiate pickup services Once the pickup request has been processed REM staff will come to your lab to pick up the waste Average turnaround time is 3 5 days The following procedures must be followed in order to have hazardous waste removed from campus locations 1 Prior to pick up all waste must be placed in a designated are
25. have a shelf life of one to two years depending on the vendor Over time moisture can slowly enter the cylinder which initiates corrosion As the corrosion continues HF and or HBr slowly react with the internal metal walls of the cylinder to produce hydrogen The walls of the cylinder weaken due to the corrosion while at the same time the internal pressure increases due to the hydrogen generation Ultimately these cylinders fail and create extremely dangerous projectiles and a toxic gas release Figure 5 8 shows a 30 year old HF lecture bottle cylinder that exploded in a Purdue University laboratory in 2011 43 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan Figure 5 8 HF Cylinder Incident at Purdue University in 2011 5 9 Cryogenic Liquids Safety A cryogenic liquid is defined as a liquid with a normal boiling point below 150 C 240 F The most common cryogenic liquid used in a laboratory setting is liquid nitrogen By definition all cryogenic liquids are extremely cold Cryogenic liquids and their vapors can rapidly freeze human tissue and can also pose an asphyxiation hazard if handled in confined spaces The following precautions should be taken when handling cryogenic liquids e Use and store cryogenic liquids in well ventilated areas only e Wear appropriate PPE while handling cryogenic liquids Proper PPE for handling cryogenic liquids includes chemical splas
26. idea is that each Laboratory Safety Officer attends departmental safety committee meetings and reports issues back to the lab and e Provides good marketable experience for the Laboratory Safety Officer to be involved in a safety leadership role The role of the Laboratory Safety Officer should include 1 Provide training to new lab personnel ensure appropriate training is given and that the training is properly documented Enforce lab safety rules Attend departmental college level safety committee meetings and report significant information back to the lab and 4 Report safety issues back to the PI when necessary 1 4 4 Non Laboratory Personnel Support Staff Responsibilities Custodians and maintenance staff support staff often must enter laboratories to perform routine tasks such as cleaning and equipment maintenance Support staff members are expected to follow the posted safety rules of each laboratory Minimum PPE requirements for support staff working in a laboratory are safety glasses long pants and closed toe shoes If additional PPE is required or if other unique safety requirements must be followed it is the lab personnel s responsibility to notify support staff 1 4 5 Chemical Hygiene Officer Responsibilities The Chemical Hygiene Officer who is the Director of the Purdue Radiological and Environmental Management Department or designated individual s has the primary responsibility for ensuring the implementation
27. into grounded receptacles Adapters that interrupt the continuity of the equipment grounding connection may not be used e All portable electric equipment and flexible cords used in highly conductive work locations such as those with water or other conductive liquids or in places where 58 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan employees are likely to contact water or conductive liquids must be approved for those locations e Employee s hands must be dry when plugging and unplugging flexible cords and cord and plug connected equipment if energized equipment is involved e If the connection could provide a conducting path to the employee s hands e g if a cord connector is wet from being immersed in water the energized plug and receptacle connections must be handled only with insulating protective equipment e Lamps for general illumination must be protected from breakage and metal shell sockets must be grounded e Temporary lights must not be suspended by their cords unless they have been designed for this purpose e Extension cords are considered to be temporary wiring and must also comply with the section on Requirements for Temporary Wiring in this program 5 18 3 Temporary Wiring Requirements Temporary electrical power and lighting installations 600 volts or less including flexible cords cables and extension cords may only be used during and for re
28. is found in the laboratory and the composition is unknown it should be assumed to be hazardous and labeled as Hazardous Waste awaiting proper characterization Figure 7 1 Purdue University Hazardous Waste Label 71 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management 7 3 Waste Storage Requirements Hazardous waste containers in Purdue laboratories are stored in satellite accumulation area SAA SAAs are used to manage hazardous waste in laboratories and shops because doing so provides safe and effective means to accumulate hazardous waste before removal by REM Additionally SAAs provide the least restrictive regulatory option for the accumulation and storage of hazardous waste containers The following SAA rules must be followed at all times when managing hazardous waste in a laboratory e All waste must be stored in containers e Containers must be in good condition and compatible with the waste they contain no corrosive waste in metal containers e Containers must be kept closed at all times except when adding or removing waste e Containers must be labeled or clearly marked with words that describe the contents of the waste e g liquid chromatography waste and the words Hazardous Waste e Containers must be stored at or near the point of generation and under the control of the generator of the waste wastes should remain in the same room they were g
29. minutes and consult a physician Seek immediate medical attention If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person If victim is conscious and alert rinse mouth out with water Seek immediate medical attention Chapter 18 Section 8 Special Handling and Storage Requirements e Do not make excessive amounts of base bath solution only make what can be safely stored in the laboratory Tab 2 Lab Specific Protocols Requirements Rules e Containers should be labeled appropriately Label should indicate the name of the chemical s in the container Avoid using chemical abbreviations acceptable if a legend is present in the lab and formulae e Glassware with excessive grime should first be rinsed with an appropriate solvent such as water and a little acetone Collect the rinsate in a separate container label with all constituents and submit to REM as waste e Ifthe glassware is greased excess grease should be removed with a paper towel e Do not place broken glassware in the base bath as this may break the glass completely and produce glass shards Check all items before placing in the bath e Do not leave glassware in the base bath for more than one overnight period Prolonged soaking in the bath will lead to degradation and consequent thinning of the glass e Always use inside a chemical fume hood Take care not to cause the bath to overflow The base
30. ne aR 22 4 1 3 Laminar Flow Clean BENCHOS oscicesventtactcetuctnk cea iecaee hes baarkeahcacs ea We otaaeans 23 AVA BIOlOZICAl SATeLY Cabinets irnia aaar E ARA sa seadsoure E OOE 23 4 1 5 Safety Showers and Eyewash Stations ccccssscccsssccccssececesececesceceeneceseeecesseneessees 24 ANG FPO HEXCIMSUISMONS eat tcat tect a reek a tA at da he ata ae ak tee at dae ee ue 25 AE FIEDOR aeran E E E ETE 25 4 2 SROMUIMISTFatIVE COMLI ONS crna a a ohwansers 26 AAL Standard Operating Proce Wk CS viii ecann TATT eet 26 AAA Required Laboratory POsStNESainedinisen rn A A 27 4 3 Personal Protective Equipment PPE sssessseesesseeesssrersssreresserresserresserressreresseeressreresseeee 28 i The official version of this document will only be maintained online Table of Contents Chapter 5 Laboratory Management Plan ccccscsscscscsscsccccsccccsceccsceccscsceccsceccsceccsceseeces 29 Dil LAD OFALOrY Safety GUIGECIINGS erruin a NENA N NAA 29 SL Laboratory Safet OUESHONS nissan A a 29 SLZ General Laboratory Safety RUIE Sinia E A RAA A N A 30 5 2 Housekeeper E ENA 31 S Chemical INVENTOR CS yesen N N A aun eat ances 32 54 Safet Data SNEER A T E 32 5 3 Chemical Labeling RegurementS neriasi naa a O A 33 5 6 Chemical Segregation kesni a a N a a ee ae 33 57 Chemicalstorage Requirement eisrunminn an a a a E cies 35 57 1 General Chemical Storage cv ccunieinaren iai T A 35 S72 Flammable TIGUIGS Storage rorirori a E E TO 36
31. only be maintained online Chapter 5 Laboratory Management Plan 34 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan Table 5 1 Chemical Compatibility Chart a a nd inorganic organic oxidizer inorganic organic inorganic organic solvent Acid fone S x xX x x vom KT eT organic owane XK XT oxidizer Base fnommne xX x of of fx Base ae eca inorganic Toxic ae a solvent 5 7 Chemical Storage Requirements Proper storage of chemicals is an essential component to a laboratory safety program Improper chemical storage practices can cause undesired chemical reactions which may form hazardous products that can lead to employee exposure or possibly fires and property damage All lab employees should carefully read each chemical s SDS and container label before deciding how to store a chemical as these will often indicate any special storage requirements that may be necessary The following subsections describe chemical storage requirements in more detail 5 7 1 General Chemical Storage The following general chemical storage guidelines must be followed in all laboratories e Each chemical in the laboratory must be stored in a specific location and returned there after each use Acceptable chemical storage locations may include flammable cabinets corrosive cabinets laboratory shelves or appropriate laboratory refrigerat
32. pdf 70 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management 7 2 Waste Identification and Labeling All chemical constituents in a hazardous waste container must be identified by knowledgeable laboratory personnel Not only is this required by the EPA it also ensures that waste can be properly characterized and disposed of by REM If there is uncertainty about the composition of a waste stream resulting from an experimental process laboratory employees must consult the laboratory supervisor for assistance In most cases careful documentation and review of all chemical products used in the experimental protocol will result in accurate waste stream characterization Additionally review SDSs specifically Section 2 Hazard Identification and Section 13 Disposal Considerations to obtain information about hazardous constituents and characteristics All waste should be properly labeled as soon the first drop of waste enters a waste container Containers must be labeled and clearly marked with words that describe the contents of the waste and the words Hazardous Waste Hazardous waste should be listed completely on the label provided by REM in a percentage format as shown in Figure 7 1 Listing accurate percentages is not as important 5 is acceptable and constituents less than 1 can be listed as trace as listing all of the chemicals that makeup the waste If a chemical
33. point lt 23 C Highly flammable liquid sa Danger Boiling point gt 35 C and vapor l l Flammable liquid and Flash point gt 23 C and lt 60 C Warning vapor Flash point gt 60 C and lt 93 C Warning Combustible liquid There is no Category 5 for flammable liquids 2 2 National Fire Protection Association Rating System The NFPA system uses a diamond shaped diagram of symbols and numbers to indicate the degree of hazard associated with a particular chemical This system was created to easily and quickly communicate hazards to first responders in the event of an emergency situation These diamond shaped symbols are placed on chemical containers to identify the degree of hazard associated with the specific chemical or chemical mixture The NFPA system is a common way to identify chemical hazards and should be understood by laboratory employees The NFPA 704 numerical rating system is based on a 0 4 system 0 meaning no hazard and 4 meaning the most hazardous note this in contrast to the GHS system where 1 is the most hazardous and 4 is the least hazardous Figure 2 4 illustrates the NFPA hazard rating system and identifies both the hazard categories and hazard rating system 13 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems Fire Hazard Health Hazard Reactivity Hazard ACID Specific Hazard Health Hazard Fire Hazard Reactivity Hazard Spec
34. regarding sharps including biologically contaminated sharps can be found at the found in the REM Sharps and Infectious Waste Handling and Disposal Guidelines http www purdue edu rem home booklets sharps pdf 7 9 Liquid Chromatography Waste Liquid chromatography LC is an analytical technique used to separate identify quantify and purify individual components of a mixture This technique is very common in biological and chemical research The most common type of LC at Purdue is High Performance Liquid Chromatography HPLC Purdue has numerous LC instruments located in laboratories all over campus Because organic solvents e g methanol acetonitrile are commonly used in the process most LC waste is regulated by the EPA as hazardous waste Consequently all containers collecting LC waste must remain closed while the LC unit is in operation It is neither 78 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management acceptable to place a waste line running from the LC unit into an open waste container nor is it acceptable to use foil or Parafilm as a means of closure as shown in Figure 7 8 Open Waste Foil SS Parafil Figure 7 8 Improper LC Waste Collection Practices One of the following practices must be employed in order to comply with hazardous waste regulations for LC waste collection systems 1 Purchase an engineered container and or cap designed for LC w
35. room or flow hood The gas cylinder should be mounted to the wall or counter the backflash valve and regulator should be attached according to manufacturer instructions The tubing should then be attached to the regulator and the quartz tube torch If practical turn off the white overhead lights The yellow lights should provide adequate illumination and will make it easier to see the flame Make sure the annealing station was left in the proper state Check that the pin valve and main valve are closed turned clockwise until tight and the regulator is at low pressure turned counterclockwise until it turns easily Start the hydrogen gas flow and light the flame Open the main valve by turning counterclockwise a couple of turns Open the pin valve by turning counterclockwise a couple of turns Hold the quartz tip where it connects to the tubing to ensure it doesn t fly off if the hydrogen flow increases suddenly Increase the regulator pressure by slowly turning clockwise until the hydrogen flow is just audible Use the round file gas lighter to start the flame If needed increase the regulator pressure until the flame is 6 long Tab 2 Lab Specific Protocols Requirements Rules Then partly close the pin valve until the flame is 2 3 long Heat the stage until it is hot enough to drive off any water that condenses on the surface Slowly pass the flame back and forth across the entire surface of the metal plate until
36. training within the last one year have read and understand the content of this SOP Tab 2 Lab Specific Protocols Requirements Rules 5 m Click here to enter text Click here to enter D R Click here to enter text Click here to enter D R Click here to enter text Click here to enter a Fr al Click here to enter text Click here to enter D nine Click here to enter text Click here to enter G nine Click here to enter text Click here to enter i nina Click here to enter text Click here to enter i onan Click here to enter text Click here to enter i enna Click here to enter text Click here to enter a nina Click here to enter text Click here to enter ae nn ean Click here to enter text Click here to enter a nn anne Click here to enter text Click here to enter ie nn anna Click here to enter text Click here to enter i nn anne Tab 2 Lab Specific Protocols Requirements Rules Standard Operating Procedure Compressed Gases This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Chapter 25 Section 1 Lab Specific Information Department Chemistry Date SOP was written 20141014 Date SOP was approved by PI lab supervisor 20141014 Pri
37. units with a certified safety program An ISP certified safety program must have the following elements e Regular safety committee meetings e Means of communicating safety issues to the department in a timely manner e Upper administrative support for safety e Self audits checklists which is a self inspection program must be completed for all areas e Abatement of deficiencies found during the self audits e An annual safety program audit and walk through by REM and e Recommendation for ISP certification renewal from REM More information about the ISP program can be found on the REM website http www purdue edu rem home files ispinfo htm 1 7 Chemical and Laboratory Safety Committee Purdue University has established the Chemical and Laboratory Safety Committee CLSC with the responsibility to promote safe and proper chemical management at all Purdue University Campuses and related facilities Chemical management includes but is not limited to the procurement and the safe handling use storage and disposal of chemicals The CLSC reviews lab safety programs and makes recommendations to the Provost as appropriate The CLSC consists of members appointed from the faculty and staff of the major research teaching and service areas where chemicals are handled or used Although REM facilitates the content of the CHP it is ultimately the responsibility of the CLSC to approve changes and updates to the CHP 8 The official version o
38. 1 or visit the REM Training webpage http www purdue edu rem home files training htm 9 1 CHP Training As discussed in Chapter 1 of the CHP all laboratory employees Pls graduate students lab technicians managers post docs visiting scientists etc must receive documented CHP training before any work with hazardous materials occurs The Laboratory Supervisor is responsible for providing CHP training Initial CHP training should include the following e Review the lab specific CHP in its entirety e Review lab specific hazard assessments e Review lab specific SOPs e Review any other lab specific protocol or requirements Refer to Appendix A for the CHP Awareness Certification Form which can be used to document CHP training 9 1 1 Annual CHP Refresher Requirements After receiving the initial documented CHP training all lab employees must receive annual CHP refresher training as well This annual refresher training can be a condensed version of the initial CHP training but should include at least the following elements e Review of the lab specific hazard assessments review of PPE requirements e Review of the lab specific SOPs e Review of any additional lab specific rules and requirements e Review of chemical spill and lab emergency procedures 82 The official version of this document will only be maintained online Chapter 9 Training 9 2 PPE Training As discussed in Chapter 6 of the CHP Laboratory Supervisors must
39. 1910 132 29 CFR 1910 140 29 CFR 1910 95 As briefly discussed in Chapter 4 of the CHP PPE should never be used in place of engineering and administrative controls 6 2 Scope This Laboratory PPE Policy applies to all personnel that work with or around hazardous chemicals or other safety and health hazards This policy is a part of the larger all encompassing Purdue PPE Policy that applies to all areas not just laboratories of the West Lafayette Campus regional campuses research farms and agricultural centers and related facilities and operations This Laboratory PPE Policy does not cover all potential hazards e g confined space entry welding operations and high voltage in all operations or settings If a laboratory encounters hazards not covered in this Laboratory PPE Policy then refer to the Purdue University personal Protective Equipment PPE Policy for more information or contact REM at 765 49 46731 for assistance http www purdue edu rem home booklets PPEPolicy pdf 6 3 Hazard Assessment The hazard assessment is a process of identifying the hazards associated with a defined task and prescribing PPE along with other relevant protection measures that must be employed to minimize the risk from the hazards Hazard assessments are performed by completing a certification of hazard assessment which is a written document detailing the hazard assessment process for defined tasks The Laboratory Supervisor is responsible for ensuri
40. 5 Laboratory Management Plan e Flexible cords and cables must be protected from accidental damage Sharp corners and projections are to be avoided Flexible cords and cables must be protected from damage when they pass through doorways or other pinch points 5 18 4 Wet or Damp Locations Work in wet or damp work locations i e areas surrounded or near water or other liquids should not be performed unless it is absolutely critical Electrical work should be postponed until the liquid can be cleaned up The following special precautions must be incorporated while performing work in damp locations e Only use electrical cords that have GFCls e Place a dry barrier over any wet or damp work surface e Remove standing water before beginning work Work is prohibited in areas where there is standing water e Do not use electrical extension cords in wet or damp locations and e Keep electrical cords away from standing water 60 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy Chapter 6 Laboratory PPE Policy 6 1 Purpose The purpose of this Laboratory Personal Protective Equipment PPE Policy is to ensure that all Purdue lab employees are aware of the PPE requirements and procedures to adequately protect themselves against chemical radiological biological or mechanical hazards This policy has been prepared in accordance with the requirements of the OSHA PPE regulations 29 CFR
41. 5 3 C mptessed Gases Storag Esener e n E D 37 574 Reactive Maternals StOl de E sridi n a otee ae etlG aaree ciated pete seaeate 38 5 7 5 lt Actitely Toxic Materials Storage esris a e Apaareacaieeink 39 5 7 67 Corrosive Materiais Storage monii En nana aE EE AAE 40 5 7 7 Oxidizers and Organic Peroxide Storage ccccssscccessecccsecccesceceenceceeecesaeeceseeeeeees 40 5 7 8 Refrigerators and Freezers Chemical Storage ccccccsssecccssccceeseeeeeseceeeeceseeeeeees 41 5 8 Compressed Gas Cylinder Safety aecncaniren a T a T ed area aes 42 529 Cogen Liquids Sal GLY siyenit RE E E A EAE AO E NEOR 44 S TONaNODartce SATCLY eaa anaa a a aaea dees ainda a a oa a a 45 S Le SMANOS Handling SALOU aossen E T 45 5 12 Equipment Apparatus and Instrument Safety cccccsccccssececesececesccceeseceseeeceseeeessees 46 SZT CENU CS eine A E aA aaa 46 S142 2 Stinrine and WIM EGUMEN aeri T T NA A 47 DZS Ca tie DEVICES arria a O a mh a O 47 5 12 4 Distillation and Solvent Purification SYStEMS cccceccccssecccesscceeeseeceeseceeaeecesseeeees 48 S25 Laboratory Glaso Wale esun RA E E E 49 S20 AEN Pressure SYSTEMS ocat a a a 49 LAA Vac Uun oV TaS aen a a r ctam eons cniaconaueus 50 5 13 Research Samples and Chemicals Developed in the Lab eecccesscccesseeeceseceeeeseeseees 51 514 Transporting Hazardous Chemicals secssttcagseucsecsvavevancneresveneuctesasadacsacatadecanceedesmamsainandn beens 52 5 14 1 Shipping Hazardous Chemic
42. 73 Chemistry raining Records Your supervisor may want copies kept in the group as well By signing below you acknowledge that you are aware of the Chemical Hygiene Plan and the policies and procedures applicable to the OSHA Occupational Exposure to Hazardous Chemicals in Laboratories Standard 29 CFR 1910 1450 Your supervisor will provide additional information and training as appropriate Name Work Telephone Email Address Department Job Title Employee Signature Date Filing Completed Chemical Hygiene Plan Awareness Certifications are to be filed in a central administrative location within each staff member s department These and all other safety training records should be organized in a way that allows original records to be retrieved quickly and efficiently on request by an OSHA inspector or a REM staff member and to be retrieved for a single staff member or for an entire work group identified by PI supervisor 86 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions Appendix B OSHA Hazard Class Definitions 87 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions B 1 Physical Hazards B 1 1 Flammable Liquids Flammable hazards are materials which under standard conditions can generate sufficient vapor to cause a fire in the presence of an ignition source Flammable liquids e g hexane e
43. 76 7 61 Labelne UNKNOWN Chemical uitein e te teactiasdetanas 76 7 6 2 Identifying Unknown ChemicalS sessssseeesseesssenssserssrrrsseerssresssrrsssrresrerssrerssrerserrese 76 7 6 3 Removing Unknown Chemicals from the Work Area cccssscccesseeeeeseceeaeeceeeeeeeees 77 A64 Pr ventine Unknown Chemical Sininsrnsiineisusnen ian a a 77 Tak SINK ANG Trash DISPOSA lsienna e arab cnacadeistag shade maa tunsaouvatabiacanen these ta ee masteacouaatenes 78 Fe NAPSAS 8 oe nn nee ot a one oP a ees ne ee ear ae ee ers ee 78 79 Ligua Chromatography Waterin a 78 Chapter Chemical SINS earcsinaranannan aa ences sawedennsetiweuenenesclaces 80 8 1 Non Emergency Chemical Spill ProceQures cccssccccssscccesseccceseceeeesceceeseeeeeeecesseseessees 80 8 2 Emergency Chemical Spill ProceQures ccccsssccccssecccesececescceeesceseesceceeseeeseecesseeeetsees 80 Boe Chemical SUN ACES onsen asus ate causes gaa tc hata E eee cena wee ae 81 CHap TAN srcsaae sinners cea satce a tecacee vacecancswnconacencecosnceseac ec E ieee teemiecanec ence 82 9T CHP TENNE are scien a wise asitiew crete nates etocnce cin agate means gia eaten A S 82 9 1 1 Annual CHP Refresher Requirements cccccssscccessecceesececeececeececeeaecesaeecesseeeeees 82 D2 PPE TEINTE Pcs ic esate spit E are E ue tence eg ae ne ania eee 83 9 3 SSOP Tanin E ea A a A encanto 83 9 4 Laboratory Chemical Safety Course CHM 605 sesssessssssssesrsssresssrrsssrresrrresrres
44. Hydroxide Solutions This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Chapter 11 Section 1 Lab Specific Information Department Chemistry Date SOP was written 20141013 Date SOP was approved by PI lab supervisor 90141013 Principal Investigator Dr Shelley Claridge Internal Lab Safety Coordinator Lab Manager David McMillan Lab Phone 4 9134 Office Phone 4 6070 Emergency Contact David McMillan 4 9134 Name and Phone Number Location s covered by this SOP 4144 AND 4144a Building Room Number Chapter 12 Section 2 Type of SOP Process Hazardous Chemical Hazardous Class Chapter 13 Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS N A Tab 2 Lab Specific Protocols Requirements Rules GHS Classification Flammable Corrosive Molecular Formula N A Form physical state Liquid Color N A Boiling Point N A Uses Base baths are highly concentrated alcoholic hydroxide solutions used to clean glassware after use The solutions consist of Ethanol or Ilsopropanol and Sodium or Potassium hydroxide The glassware is cleaned by chemically dissolving contaminated surfaces
45. Labeled Unknown Waste 7 6 2 Identifying Unknown Chemicals Every effort should be made by laboratory personnel to identify unknown chemicals Here are a few steps that can be taken to help this effort 76 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management 1 Ask other laboratory personnel if they are responsible for or can help identify the unknown chemical 2 The type of research conducted in the laboratory can be useful information for making this determination Eliminating certain chemicals as a possibility helps narrow the problem as well This is especially important for mercury PCB or dioxin compounds because they must be managed separately from other hazardous waste 3 For trade products contact the manufacturer or search online to obtain an SDS REM staff can assist you in finding an SDS 7 6 3 Removing Unknown Chemicals from the Work Area If it is not possible to identify the material a Hazardous Waste label should be placed on the container as described above in Section 7 6 1 and a Hazardous Materials Pickup Request Form should be submitted which describes all of the available information e g 4 liter container of clear liquid Call REM at 765 49 40121 if you have a question about an unknown 7 6 4 Preventing Unknown Chemicals Here are a few tips that will help prevent the generation of unknown chemicals e Label all chemical containers including beakers
46. Solids A flammable solid is a solid which is readily combustible or may cause or contribute to a fire through friction Readily combustible solids are powdered granular or pasty substances which 88 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions are dangerous if they can be easily ignited by brief contact with an ignition source Flammable solids are more hazardous when widely dispersed in a confined space e g finely divided metal powders B 1 3 Gases under Pressure Gases under pressure are gases which are contained in a receptacle at a pressure not less than 280 kPA at 20 C or as a refrigerated liquid Gases under pressure include the following e Compressed gas is a gas which when packaged under pressure is entirely gaseous at 50 C including all gases with a critical temperature lt 50 C e Liquefied gas is a gas which when packaged under pressure is partially liquid at temperatures above 50 C e Refrigerated liquefied gas is a gas which when packaged is made partially liquid because of its low temperature e Dissolved gas is a gas which when packaged under pressure is dissolved in a liquid phase solvent All compressed gases are hazardous due to the fact they are stored in compressed cylinders which can explode and act as a projectile if ruptured Compressed gases also carry the hazards of the chemicals they contain such as asphyxiation carbon di
47. a within the room where the waste was generated All waste must be placed in an appropriate container s All containers must be capped and labeled Complete and submit a Hazardous Materials Pickup Request Form Figure 7 6 Visit the REM webpage to find the online Hazardous Material Pickup Request submission form For further information regarding hazardous waste disposal call REM at 765 49 40121 or visit the REM webpage http www purdue edu rem hmm hmm htm HMM 001 l cs Hazardous Material Pickup Request i Armure materiais must be submittedina o P percentage format Each Container s percentage must Send to REM LMSB via campus mail or Fax 49 61106 add up to 100 See Example Below 2 Chemical descriptions on this form must match the container label PI PI Telephone e Always include as much information as possible e Only submit known mixtures or chemicals e Absolutely no chemical formulae abbreviations Req uested By Date acronyms or structures When submitting trade products Telephone E Mail e It is your responsibility to provide the product information me e An accurate chemical description of the product Building Room Shop must accompany the form i e an MSDS MARAEA ROCRUON Make sure all writing is legible Department Dept Code Forms completed improperly can cause severe delays in pickup times Example Pickup Line Acetone 50 Benzene 10 Dichlorometh
48. afety reminders e Be sure to wear goggles while annealing e Direct the flame away from yourself and anyone else e Do not ignite the flame before you turn off the lights e When done blow out the flame turn off the tank vent the rest of the gas and turn off the regulator Tab 2 Lab Specific Protocols Requirements Rules Protocol for Changing the Lab 180L Nitrogen Tank This is a relatively straightforward procedure but there are a couple of things one needs to keep in mind to prevent complications First an overview of the tank 1 Gas Use Valve This is the valve that controls the influx of nitrogen gas into the main line of the lab 2 Vent Valve This valve is connected to a device called the whisper valve It basically prevents pressure from building up too high and releasing through the safety valve very loudly Tab 2 Lab Specific Protocols Requirements Rules Pressure Builder Valve This valve controls the flow of liquid nitrogen WITHIN the tank itself allowing one to convert it into gas to rapidly build pressure if one desires normally not used NOT SEEN Liquid Valve Controls the release of liquid nitrogen Now that we know what the four valves are we can begin discussing how to disassemble everything and then reassemble it Disassembly a Close the gas use valve completely remember righty tighty lefty loosey b Close the vent valve completely c The pressure builder valve should already be close
49. al Storage A number of general precautions need to be taken when storing chemicals in refrigerators and or freezers in the laboratory When working with freezers or refrigerators the following procedures must be followed e Domestic refrigerators or freezers must never be used to store eS ae ae VWREY flammable liquids Flammable liquids are only allowed to be stored in refrigerators or freezers that are designed for AWARNING SKEEP FIRE AWAY flammable materials most refrigerators are not intended for flammable storage e Lab refrigerators or freezers must never be used to store food or beverages for consumption Lab refrigerators freezers should be posted with a sign that states No Food or Drink e All chemicals stored in a refrigerator or freezer must be labeled e Ensure that the chemicals stored in a refrigerator or freezer is compatible with each other For example do not store an oxidizer such as hydrogen peroxide in a refrigerator with organic chemicals ae e There must not be any open chemicals in a refrigerator or freezer All containers must be completely sealed or capped and safely stored e Chemicals should be allowed to warm to room temperature before sealing to prevent pressure buildup 41 The official version of this document will only be maintained online 5 8 Chapter 5 Laboratory Management Plan e Shelves in refrigerators or freezers should all have suitable plastic trays for secondary containme
50. als Off Campus cccccsssecccssecccessceeeeseeeeeseceseeecesseneeees 53 5 14 2 Transporting Chemicals on Campus via Purdue VeNICI ccccceeccceesccenecteeeeeeees 53 5 14 3 Transporting Chemicals On Campus via FOOt ccscccssececessceceseeeeeeceeeeecesseeeeees 55 5 15 ka OratOry Seli INSOCCUONS cctssitwiienisacesesscd tes vaaceceus Piteasie hash sea vibe a ana ae era 56 De LG LAD OFALORY ERGONOMICS 2 cucu E A oacamaipencammeneec nen wean tna tanseayanseucaneseae 57 5 17 Laboratory Electrical SAT OLY sec cassaeceGaccaceeciavencendsainioasianSehacet doectavacseecesaaatenwieniceacehisetnaeeeees 57 so DF as TR NNE o E E E O E ee een 57 5 17 2 Portable Electrical Equipment and Extension CordS sssssssssssssreseressrresrererrerrreeeees 58 SA73 lemporary Witme Reguire meN S seror a E T 59 5 174 Wetor Damp LOCatiON Serii as E EAE AE A a 60 ii The official version of this document will only be maintained online Table of Contents Ghanter 6 Laboratory PRE PONG sicesccaecciesetceasib secs aa a aa 61 OT FPUrPOSE natron den sens Sees aavecndes staan eset sae O suuan sens Sacames ivaaneeeseeenceas 61 O2 o ODE n geo Sree mes ene ne ttn Tre rr GREET coe NERS CCRT Cn ens ne eee aren eee cere eee ae 61 eo MaL Cl NS SS SM serstete ce eat ecctasiaaerna eu tets NONE NE 61 6 3 1 Task Evaluation Hazard Assessment ccccssecccessceceesececeececeesceeeececeeaecesauecesseeeees 62 63 2 Location Evaluation Hazard ASSESSIMENE serc
51. also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle should not be exposed Aprons may also be appropriate depending on the application Hygiene Measures Tab 2 Lab Specific Protocols Requirements Rules Wash thoroughly and immediately after handling Rinse immediately contaminated clothing and skin with plenty of water before removing clothes Chapter 16 Section 6 Engineering Controls Preparation and use of base baths should be conducted in a properly functioning chemical fume hood whenever possible The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet per minute Chapter 17 Section 7 First Aid Procedures If inhaled Move into the fresh air immediately Consult a physician If not breathing give artificial respiration and seek immediate medical attention In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Consult a physician If skin irritation or dermatitis develops seek immediate medical attention In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15
52. ammable corrosive oxidant or reactive Call REM at 765 765 49 40121 for assistance with identifying the hazards of samples If samples are consolidated for storage e g vial boxes it is not always necessary to label every sample container For example a box containing sample vials which are all in the same hazard class e g miscellaneous pharmaceutical compounds considered to be toxic can have one label on the outside of the box stating Miscellaneous Toxic Pharmaceutical Compounds or a similar description A label such as the one shown in Figure 5 9 can be used to identify consolidated samples and should only be used ona temporary basis This type of information communicates the hazards to emergency responders as well as gives REM the information necessary for proper disposal If the chemical substance is produced for another user outside of the lab the Laboratory Supervisor must comply with the Hazard Communication Standard including the requirements for preparation of SDSs and container labeling Chemical Description sE RA lan Employee Name Date 0 10 2013 Circle the hazard s that apply Flammable Reactive Oxidizer Corrosive Explosive Irritant Non Haz Figure 5 9 Example Temporary Sample Container Label 5 14 Transporting Hazardous Chemicals Transporting chemicals is a potentially hazardous process that must be done properly to avoid accidents and potential injuries The following subsections
53. an be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Additional lab protocol may be added here Claridge lab has 3 compressed gasses currently Hydrogen for flame annealing refer to document Hydrogen Flame annealing below High purity nitrogen for sample blow off Receive training to use this simple system Glove box catalyist cleaner 94 N 4 H only lab safety coordinator should use this If you think the glove box need regeneration please inform them NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with compressed gases designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures e The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer e The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP mre Click here to enter text Click here to enter a date Click here to enter text Click here to e
54. an lethal concentration LC5O in air of 200 parts per million ppm by volume or less of gas or vapor or 2 mg per liter or less of mist fume or dust when administered to certain test populations by continuous inhalation for one hour provided such concentration and or condition are likely to be encountered by humans when the chemical is used in any reasonably foreseeable manner Chapter 5 7 5 of the CHP details acutely toxic compounds storage requirements 94 The official version of this document will only be maintained online Appendix C Peroxide Forming Chemicals Appendix C Peroxide Forming Chemicals 95 The official version of this document will only be maintained online Appendix C Peroxide Forming Chemicals Autoxidation in common laboratory solvents can lead to unstable and potentially explosive peroxide formation The reaction can be initiated by exposure to air heat light or contaminants Most of these solvents are available with inhibitors to slow the peroxide formation Examples of inhibitors include BHT 2 6 di tert butyl 4 methyl phenol and Hydroquinone There are three categories of peroxide formers Group A chemicals are those which form explosive levels of peroxides after prolonged storage especially after exposure to air without concentration Test these for peroxide formation before using and discard 3 months after opening Table C 1 Group A Chemicals Butadiene Isopropyl ether Chloroprene Tetrafl
55. and seek immediate medical attention In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes Wash any contaminated clothing before reuse Thoroughly clean shoes before reuse Consult a physician In case of eye contact Check for and remove any contact lenses Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician Seek immediate medical attention If swallowed Do NOT induce vomiting unless directed by medical personnel Never give anything by mouth to an unconscious person Seek immediate medical attention Section 8 Special Handling and Storage Requirements e Compressed gas cylinders should be stored in a secure well ventilated location and in an upright position at all times e All compressed gas cylinders should be handled as if full and should never be completely emptied e Cylinders that are not in use meaning that the regulator is not attached must be secured and the safety cap must be on the cylinder and are permitted to be chained together as shown below in Figure 1 e Cylinders that are in use meaning there is a regulator attached must be individually secured by a chain or strap as shown below in Figure 2 e Cylinder valves and regulators must be protected from impact or damage e A designated storage area must be established for compressed gases e Toxic highly toxic corrosive and reactive gase
56. and students in maintaining a safe environment in which to teach and conduct research Each laboratory using hazardous materials is required to have a copy of the CHP readily available to all laboratory personnel Each laboratory worker must be familiar with the contents of the CHP and the procedures for obtaining additional safety information needed to perform their duties safely 1 1 Purpose Purdue University is committed to providing a healthy and safe work environment for the campus community The Purdue University CHP establishes a formal written program for protecting laboratory personnel against health and safety hazards associated with exposure to hazardous chemicals and must be made available to all employees working with hazardous chemicals in a laboratory setting The CHP describes the proper use and handling procedures to be followed by faculty staff and all other personnel working with hazardous chemicals in laboratory settings 1 2 Scope The CHP applies to all laboratories that use store or handle hazardous chemicals and all personnel who work in these facilities The information presented in the CHP represents best practices and provides a broad overview of the information necessary for the safe operation of laboratories that utilize hazardous chemicals Laboratory use of hazardous chemicals is defined as handling or use of such chemicals in which all of the following conditions are met Chemical manipulations are carried out on
57. ane 20 Acetic Acid 10 Water 10 Trace Silver os eee gal S a I have made a good faith effort to minimize my waste generation and select the best waste management method that is available to me and that can afford Amount Solid Chemical Description List Each Container patie plo eee of ee ore me Container a Aerosol I j 4 Figure 7 6 Hazardous Materials Pickup Request 75 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management 7 6 Unknown Chemical Waste Unknown chemicals are a serious problem in laboratories Mysterious chemicals are often stored in labs for years before lab personnel notice the unidentified items However steps can be taken to assist with proper management of unknowns Unknown chemicals must be properly identified according to hazard class before proper disposal The hazards that should be noted include corrosive flammable oxidizer reactive toxic and radioactive The following subsections describe in detail how to properly manage unknown chemicals 7 6 1 Labeling Unknown Chemicals Until the unknown chemical can be properly identified by either lab staff or REM the container should be labeled with a Hazardous Waste Disposal Tag The following information should be written on the label Unknown hazardous chemical awaiting proper characterization by REM as illustrated in Figure 7 7 Figure 7 7 Properly
58. ar r T A 62 6 3 3 Job Title Evaluation Hazard Assessment sseseesssessseerssrerssressrrrossrrssrerssrerssresserreses 63 6 4 Minimum PPE Requirements for Laboratories cccccessccccssececesececeseeceeseceseeeceseeecessees 63 6At Head Protectioiniranpisa n nnn a a a ae ee ae 64 6 4 2 Hearne ProtectiON cerisa E S 64 643 RESPIFALOLY Prote cHUON roi nanne N A NE 65 644 Eyeand Face Proteco epin E E E AE TO 65 645 Hand Protectio sacmiaosiinn aa adelaide E A A 65 6A BOV PrOECHON axtsenaisiaacuae tee T 66 A7 FOOU PROLECIION uria a E E EN E 67 6 5 Minimum PPE Requirements for Support Staff and VISItOTS sssssssseesssensseereseereseesssee 67 6 6 PPE TAMAS REGU ICICI ES cranio e vaio ss esac ds eee sla cag vk en Pn saeco cee eee agals 67 6 7 Injuries Illnesses and Medical Examinations cccccceccccsecccsssecenccceneceeeceeeeceseneeteneetens 68 Chapter 7 Hazardous Waste Manageme nt ccccsccccscscsscsccccsccccsceccscescccsccccsceccscescscesccees 70 At Todu OMaria bce aces eid nies E van als vem vate vais ato eta eee ee 70 7 2 Waste Identification and Labeling a ciciedicstaieci tee sncd eae aie gdtiicd da Wec onde Bissuciiedae engi cetiaceceeees 71 73 Waste Storage REGUIFCINIGIIES sxc acsncesocceincaadeseseist E eaten nitesoaomnatrentn 72 Tee Waste COMUAIN Gl Ss ctucear datrawsacteeabouceutaeecomacnaiisdeitiwin A T EA A 73 Ta Waste Disposal Procedure Sresi a a AR 75 7 6 UnKNOwN Chemical WaS teinien a T A E N A
59. aste collection Figure 7 9 shows several examples of acceptable solutions for proper LC waste collection that can be purchased Safety Cans Ported Cap No Threads 4 Port threaded Cap Solvent Bottle Cap Figure 7 9 Proper LC Waste Collection Options for Purchase 2 An existing cap can be modified by the research lab for LC waste collection To modify an existing cap a hole can be drilled into a cap The diameter of the hole should be similar to the diameter of the waste line there should be a tight fit between the container opening and waste line In addition a hole should be drilled to accommodate any exhaust filter or air valve tube that may be required It is recommended that either a 4 liter container or 5 gallon carboy be used for waste collection The modified cap should be replaced with a regular unmodified cap once the container is full and ready for REM pickup See Figure 7 10 for examples of acceptable modified caps 79 The official version of this document will only be maintained online Chapter 8 Chemical Spills Chapter 8 Chemical Spills Chemical spills in the laboratory can pose a significant risk to human health and the environment All lab personnel must be trained on how to properly respond to chemical spills in order to minimize risk In general chemical spills can be placed into one of two categories non emergency chemical spills or emergency chemical spills 8 1 Non Emergency Chemical Spill Procedur
60. atible Waste Streams 7 4 Waste Containers REM does not provide containers to campus It is the responsibility of the generator of the waste to provide containers Usually the original container of the main component of the waste can be used e g 4 liter glass jar 5 gallon green metal solvent can Purdue Stores also offers waste containers such as 20 liter carboys as shown in Figure 7 3 for sale If requested reusable hazardous waste storage containers of 5 gallons or larger may be returned to the generator s area Mark the container clearly with Return to the building and room number as illustrated in Figure 7 4 Containers unsuitable for reuse will be properly disposed of and not returned 73 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management Figure 7 3 20 Liter Carboy Figure 7 4 Reusable Waste Container Purdue s policy for the disposal of empty containers is implemented to protect Purdue facilities and the Physical Facilities Buildings and Grounds staff when removing trash Please remember that some chemical residues have the potential to mix with other incompatible residues in the dumpster or compactor causing a reaction or fire In addition sealed containers may become pressurized during compaction which may result in residues spraying onto workers Please keep the following procedures and information in mind when disposing of empty containers e Triple rinse
61. ay be associated with working in the glove box e All equipment and chemicals in the glove box must be organized and all chemicals must be labeled Do not allow items particularly chemicals to accumulate in the glove box 4 2 3 Laminar Flow Clean Benches A laminar flow clean bench as shown in Figure 4 4 is an enclosed bench designed to prevent contamination of semiconductor wafers samples or any particle sensitive device Air is drawn through a filter and blown in a very smooth laminar flow towards the user Therefore it is critical that absolutely no hazardous chemicals infectious and or radioactive materials ever be used in a laminar flow clean bench as the vapors are blown directly towards the user Applications that involve the use of chemicals should be conducted in chemical fume hoods Figure 4 4 Laminar Flow Clean Bench 4 2 4 Biological Safety Cabinets A biological or biosafety safety cabinet as shown in Figure 4 5 is an enclosed ventilated laboratory workspace for safely working with materials contaminated with or potentially contaminated with infectious materials The primary purpose of a biosafety cabinet is to serve as a means to protect the laboratory worker and the surrounding environment from 23 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls pathogens All exhaust air is filtered as it exits the biosafety cabinet removing harmful particles
62. bs it would be difficult to get enough out of our dewar to cause a problem PPE Required Safety glasses Cryogenic gloves Close toed shoes Long sleeves or a lab coat Tab 2 Lab Specific Protocols Requirements Rules Tab 3 Lab Specific Hazard Assessments Lab Hazard Assessment 2014 CHP Clardige Lab Chapter 1 ERTIFICATION OF HAZARD ASSESSMENT 9 18 5 CERTIFICATION OF HAZARD ASSESSMENT INTRODUCTION 1 Hazard assessment is the process required by law of identifying the hazards associated with defined task prescribing personal protective equipment and other relevant protection measures which must be employed to reduce the risk from the hazards Certification of Hazard Assessment is a written document such as the one on the following 2 pages detailing the hazard assessment s for a particular task s The supervisor is responsible for ensuring that hazard assessments are performed and the certification s written and posted The supervisor may delegate or contract the labor involved in this process but cannot reassign or disclaim the responsibility INSTRUCTIONS e Save the attached hazard assessment example to your hard drive e It must be modified to meet the specific hazards of your work area This includes removing or adding hazards as applicable to your work area e Certification s of hazard assessments must be posted tacked or hung in a visible place in every work room listed in the lo
63. c must be labeled If is not practical to label a secondary container that is in process e g reaction vessel flask a temporary label can be used as shown in Section 5 7 of the CHP Reaction vessels in chemical fume hoods must be labeled as well If labeling the vessel itself is not practical the hood sash or wall may be labeled as illustrated in Figure 4 2 Figure 4 2 Alternative Labeling of Chemical Fume Hood Reaction Vessels Do not allow the vents or air flow baffles to be blocked Never put your head inside of an operating chemical fume hood All chemical fume hoods should be routinely checked for airflow by measuring the face velocity which should be between 70 125 feet per minute REM conducts face velocity readings on a routine basis and records this information on the hood label Contact REM with questions regarding chemical fume hoods 765 49 46371 21 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls 4 2 2 Glove Boxes A glove box as illustrated in Figure 4 3 is a sealed container that is designed to allow one to handle material in a defined atmosphere typically inert Glove boxes can be used to protect sensitive items inside or the user on the outside or both The following recommendations should be followed by all personnel using a glove box Figure 4 3 Glove Box e All personnel must receive documented training from the PI or delegate be
64. cation s field e The fields at the beginning date s location s Supervisor and signature must be completed Post signed certification in work room 2014 CHP Clardige Lab Chapter 1 C ERTIFICATION OF HAZARD ASSESSMENT Supervisor print Dr Shelley Claridge Assessment Date s 20141012 Signature Location s posted 4144 and 4144A Task hands on work or being within reach of potential hazards of described Minimum Requirements activity items Skin eye Volume gt 10 mL any unshielded Splash goggles chemical resistant damage corrosive liquids organic liquids or gloves lab coat skin cover to poisoning liquid mixtures or toxic inorganic knees elbows throat closed shoes inhalation of liquids mixtures with socks Work in hood Shower vapor or aerosol and eyewash must be available in work area Volume gt 1L Same but cover to ankles wrists throat Volume gt 5L Add face shield covering chin Eye impact Metalworking woodworking other Safety glasses Skin eye Cryogenic liquids Splash goggles skin cover to damage elbows knees throat closed shoe easily removed socks Cryogloves for dispensing Volume gt 1L Skin cover to throat wrists ankles Skin eye Transport of liquid nitrogen in hallways See cryogenic liquids also all damage and elevators wheeled vessels or carts must asphyxiation restrain Dewar and have wheels body injury large enough to safely traverse frostbite elevator d
65. ce of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911 Chapter 20 Section 10 Medical Emergency Tab 2 Lab Specific Protocols Requirements Rules Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Chapter 21 Section 11 Waste Disposal Procedures Label Waste Base bath solutions cannot be disposed of down the drain Do not mix base bath solutions with acidic waste streams After the base bath has lost its cleaning effectiveness transfer the solution to a heavy duty container such as Nalgene bottle carboy Make sure the waste container s is properly labeled label should indicate all of the contents of the container including any potential organic contaminants from cleaning process REM provides hazardous waste labels free of charge call 49 40121 to obtain labels Store Waste Store hazardous waste in closed containers and in a designated area away from incompatible materials Dispose of Waste Complete a Chemical Waste Pickup Request Form to arrange for disposal by REM Call REM at 49 40121 or visit the REM webpage for questions http www purdue edu rem hmm wststo htm Chapter 22 Section 12 Safety Data Sheet SDS A current copy of the SDS f
66. cetic Acid Figure 5 6 Segregation Using Secondary Containment 5 7 7 Oxidizers and Organic Peroxide Storage Oxidizing materials are defined as substances which while in itself are not necessarily combustible may generally by yielding oxygen cause or contribute to the combustion of other material An organic peroxide is an organic substance which contains the bivalent O 40 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan O structure and may be considered a derivative of hydrogen peroxide where one or both of the hydrogen atoms have been replaced by organic radicals The following guidelines for storing oxidizers and organic peroxides must be followed in all laboratories e Oxidizers e g hydrogen peroxide sodium nitrate and organic peroxides e g methyl ethyl ketone peroxide benzoyl peroxide must be stored in a cool dry location and kept away from combustible materials such as wood pressboard paper and organic chemicals e g organic solvents and organic acids e If possible store all strong oxidizing agents in a chemical cabinet dedicated only for oxidizers e The amount of oxidizers and organic peroxides stored in the lab should be kept at a minimum e All material must be clearly labeled the original manufacturer s label with the chemical name hazard labels and pictograms should not be defaced or covered 5 7 8 Refrigerators and Freezers Chemic
67. chemicals from one area to another shall be Stable and in good condition Transport only a quantity which can be handled easily Plan the route ahead of time so as to avoid all steps or stairs e Freight elevators not passenger elevators should be used to transport hazardous chemicals whenever possible The individual transporting the hazardous chemicals 55 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan should operate the elevator alone if possible Avoid getting on an elevator when a person is transporting hazardous chemicals 5 15 Laboratory Security All laboratory personnel have a responsibility to protect university property from misuse and theft of hazardous materials particularly those that could threaten human health At a minimum the following security measures should be employed in all campus laboratories e The laboratory door should remain locked when not occupied e Always feel free to question anyone that enters the lab that you do not know and ask to see identification if necessary e If you see anything suspicious or someone displays suspicious behavior immediately report it to the Purdue Police Department by dialing 911 emergency or 765 494 8221 non emergency e Any sensitive information or particularly hazardous chemicals should not be stored out in the open where anyone can readily have access to them These types of materials should be stored in a s
68. cts Explosive Organic Peroxide Gas Under Pressure Corrosive Self Reactive Environmental Toxicity Acute Toxicity Severe Figure 2 2 GHS Hazard Pictograms GHS labeling requirements are only applicable to chemical manufacturers distributors and shippers of chemicals GHS labeling requirements are not required for chemicals being stored in a laboratory However since most chemicals stored in the laboratory have been purchased from a chemical manufacturer the GHS labeling and pictogram requirements are very relevant and must be understood by laboratory employees Figure 2 3 illustrates the GHS label format showing the required elements 11 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems ACETONE PRODUCT IDENTIFIER HAZARD PICTOGRAMS Code Product Name SUPPLIER IDENTIFICATION Company Name Street Address City Postal Code Phone Number SIGNAL WORD Danger HAZARD STATEMENT Highly flammable liquid and vapor Causes mild skin irritation Causes serious eye irritation May cause drowsiness or dizziness PRECAUTIONARY STATEMENTS Keep away from heat sparks open flames hot surfaces No smoking Avoid breathing dust fumes gas mist vapors and spray IF IN EYES Rinse cautiously with water for several minutes Remove contact lenses if present and easy to do Continue rinsing Repeated exposure may cause skin dryness and cracking In Ca
69. d but check and make sure d Again the liquid valve should be closed completely already but check and make sure e Remove the nozzle from the gas use valve as shown below Tab 2 Lab Specific Protocols Requirements Rules e Continued on next page f Remove the Whisper Valve from the vent valve as shown below g Remove the nozzle from the liquid valve as shown below may or may not be frosty Tab 2 Lab Specific Protocols Requirements Rules h Do not mess with the pressure building valve Now you are ready to take the tank down to be refilled Note Tank may only travel on the freight elevator Fill station is on loading dock at East end of Brown Fill is at 39m M F no other times available Pick up usually at 330 or 4 Plan ahead e Reassembly a Reattach all nozzles and valves as shown in Disassembly Makes sure they are well tightened with the appropriate wrench also shown in Disassembly b Open the vent valve Tab 2 Lab Specific Protocols Requirements Rules c Open the gas use valve Notes and Knowledge Theory Liquid nitrogen is made by rapidly lowering the pressure of gaseous nitrogen to draw off heat When the temperate drops to around 77K it liquefies For efficient long term storage more than a day it must be stored in vacuum dewars which provide excellent thermal insulation In storage it slowly boils to gas this creates pressure the pressure is relieved for safety and storage effic
70. d Assessment Form is required for all laboratories Detailed information regarding the hazard assessment process is presented in Section 6 3 of the CHP The Carcinogens Reproductive Toxins or Extremely Toxic Chemicals label Toxic Chemicals Label which is illustrated in Figure 4 6 is required if a lab uses or stores any chemicals on the list linked below Contact REM 765 49 46371 to request Toxic Chemicals Labels http www purdue edu rem home booklets crdalist pdf Figure 4 6 Toxic Chemicals Label 27 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls e The Abbreviations Acronyms and Chemical Formulas list is required for all labs that use abbreviations acronyms and or chemical formulas as a means to label chemical containers including secondary containers such as beakers flasks and vials This list which can be found on the REM webpage is not all inclusive and any abbreviations not listed must be added by laboratory personnel http www purdue edu rem home files guide htm There are several other lab postings that may also be required that are not discussed in the CHP particularly if radioisotopes and or biological agents are used in the lab This information should be obtained by reviewing the Radiation Safety Manual and or Biological Safety Manual Additional information regarding lab postings and labels can be found on the REM webpage http www purdue
71. d be cleaned routinely e g daily weekly e For operations where spills and contamination are likely e g agarose gel electrophoresis ethidium bromide applications cover work spaces with a bench paper or liner The soiled bench paper should be changed on a routine basis or as needed e All chemical spills must be cleaned up immediately Refer to Chapter 8 of the CHP for detailed chemical spill cleanup procedures e Do not allow materials to accumulate in laboratory hoods and remove used tissues foil gloves or other unnecessary objects immediately after use The safety of the workspace and the hood ventilation may be compromised when excessive chemicals and equipment are kept in hoods e Ensure that all waste e g trash chemically contaminated waste etc is placed in the appropriate containers Do not overfill waste containers e All equipment should be cleaned and returned to storage after each use e Equipment should be stored in a safe and orderly manner that prevents it from falling e Chemical containers must be clean properly labeled and returned to storage upon completion or usage Avoid storing liquids above eye level e Do not store heavy or frequently used items on top shelves Locate items used daily close to the work area 31 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 3 Chemical Inventories It is a prudent practice to develop and maintain a chem
72. d be followed when using vacuum apparatus in the laboratory e Do not allow water solvents or corrosive gases to be drawn into vacuum systems Protect pumps with cold traps and vent their exhaust into an exhaust hood e Assemble vacuum apparatus in a manner that avoids strain particularly to the neck of the flask e Avoid putting pressure on a vacuum line to prevent stopcocks from popping out or glass apparatus from exploding e Place vacuum apparatus in such a way that the possibility of being accidentally hit is minimized If necessary place transparent plastic around it to prevent injury from flying glass in case of an explosion e When using a rotary evaporator the glass components of the rotary evaporator should be made of Pyrex or similar glass Glass vessels should be completely enclosed in a shield to Pe Se guard against flying glass should the components implode Increase in rotation speed and application of vacuum to the flask whose solvent is to be evaporated should be gradual e When using a vacuum source it is important to place a trap between the experimental apparatus and the vacuum source The vacuum trap protects the pump and the piping from the potentially damaging effects of the material protects people who must work on the vacuum lines or system and prevents vapors and related odors from being emitted back into the laboratory or system exhaust The following vacuum trapping guidelines should be followed o Mak
73. dary chemical containers as well 5 6 Chemical Segregation All chemicals must be stored according to chemical compatibility Once segregated by chemical compatibility they can then be stored alphabetically Information regarding chemical compatibility can be found in the SDS primarily in Section 7 Handling and Storage and Section 10 Stability and Reactivity If unsure of proper segregation procedures contact the Laboratory Supervisor for assistance Chemical segregation can be achieved by either isolation e g organic solvents stored in a flammable cabinet physical distance e g acids and bases are stored on opposite sides of a chemical storage room or secondary containment e g placing oxidizing acids such as nitric acid into a secondary containment to segregate from organic acids such as formic acid as shown in Figure 5 7 In the most general terms proper segregation can be achieved by e Storing acids away from bases and toxics e Storing oxidizers away from organic chemicals and e Storing reactive and acutely toxic materials away from all other chemicals Table 5 1 illustrates a more detailed chemical compatibility logic that can be used for chemical storage Hazard classes marked by an X need to be segregated from each other e g Acid inorganic must be segregated from Base inorganic Contact REM at 765 49 40121 with questions regarding chemical segregation 33 The official version of this document will
74. dling storage segregation and labeling of chemicals and equipment safety must be established and known by all laboratory personnel This chapter details how laboratories should be managed at Purdue 5 1 Laboratory Safety Guidelines All laboratory employees must have a good understanding of the hazards associated with the chemicals being used and stored in the lab Basic factors such as the physical state gas liquid or solid of the chemical and the type of facilities and equipment involved with the procedure should be considered before any work with hazardous materials occurs 5 1 1 Laboratory Safety Questions Many factors are involved is laboratory safety Asking and answering the following questions will help address many of the factors that should be considered when it comes to laboratory safety e ls the material flammable explosive corrosive or reactive e Is the material toxic and if so how can be exposed to the material e g inhalation skin or eye contact accidental ingestion accidental puncture e What kind of ventilation do need to protect myself e What kind of PPE e g chemical resistant gloves respirator and goggles do need to protect myself e Will the process generate other toxic compounds or could it result in a fire explosion or other violent chemical reaction e What are the proper procedures for disposal of the chemicals e Dol have the proper training to handle the chemicals and carry ou
75. e PPE necessary for laboratory work with chemicals Regulatory agency familiarity and compliance topics including OSHA EPA DOT and NRC are also included CHM 605 is open to all departments and is a required course for some departments All graduate students working in a laboratory environment are strongly encouraged to take CHM 605 9 5 REM Researcher s Guide The CHP focuses on work with hazardous chemicals in the laboratory However other common types of hazards are present in many research labs as well e g biological hazards lasers etc REM has developed the Researcher s Guide as a tool to assist researchers with compliance and training requirements for a broad range of common hazards and regulatory programs found in the laboratory Visit the REM Researcher s Guide for more detailed information http www purdue edu rem home files researchers htm 84 The official version of this document will only be maintained online Appendix A CHP Awareness Certification Form Appendix A CHP Awareness Certification Form 85 The official version of this document will only be maintained online Appendix A CHP Awareness Certification Form PURDUE UNIVERSITY Chemical Hygiene Plan and Hazardous Materials Safety Manual Awareness Certification Please Type or Print Legibly For Principal Investigator Building and Room s After reading the Purdue Chemical Hygiene Plan complete and return a copy of this form to WTHR 1
76. e included below e REM SDS Search http www purdue edu rem ih msds htm e Siri MSDS Index http hazard com msds e Sigma Aldrich Product Search http www sigmaaldrich com united states html 32 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 5 Chemical Labeling Requirements Every chemical container present in the laboratory whether hazardous or not must be properly labeled All secondary ae seat as 1 1 eens Fe iBT SIMPPING NAMEZUNE lt ea chemical containers e g wash bottles beakers flasks sample amenes case JZE pets physcian immediately INGESTION DO nio j vials etc that are in use must also be properly labeled Avoid o orna p using abbreviations chemical formulae or structure unless there is a complete and up to date legend e g MeOH een ee ito mare pn lt Sd contamination fron ne rout CODE No Methanol prominently posted in the lab Most chemicals eam O E come with a manufacturer label that contains all of the Sace LL necessary information so care should be taken to not damage or remove these labels It is recommended that each bottle also be dated when received and when opened to assist in determining which chemicals are expired and require proper disposal Detailed information and strategies for the labeling of research samples is discussed in Section 5 9 of the CHP These same strategies can be used when labeling secon
77. e safety cap must be in place when a gas cylinder is moved For large gas cylinders gt 27 inches an approved gas cylinder cart should be used The cylinder must be strapped to the cart and the protective cap must be in place before moving the cylinder A cylinder should never be moved or transported without the protective cap The proper way to move a large gas cylinder is illustrated in Figure 5 7 Never dispense from a cylinder if it is on a gas cylinder cart 42 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan Figure 5 7 Gas Cylinder Cart A few compressed gas cylinders have a shelf life and can become more hazardous as time goes on It is extremely important that these chemicals are identified and managed properly If any time sensitive gases are found to be past the manufacturer s expiration date they must be submitted to REM for hazardous waste disposal immediately The following is a list of time sensitive compressed gases e Hydrogen Fluoride anhydrous e Hydrogen Bromide anhydrous e Hydrogen Sulfide anhydrous e Hydrogen Cyanide anhydrous e Hydrogen Chloride anhydrous The compressed gases listed above have a shelf life provided by the manufacturer that must be strictly followed There have been numerous incidents involving these compounds related to storage past the expiration date For example hydrogen fluoride HF and hydrogen bromide HBr cylinders
78. e sure the flask is properly clamped and secured o Make sure the vacuum hose is connected to the vacuum line not the gas line o To prevent contamination all lines leading from experimental apparatus to the vacuum source should be equipped with filtration or other trapping device as appropriate 50 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan o For particulates use filtration capable of efficiently trapping the particles in the size range being generated o For most aqueous or non volatile liquids a filter flask at room temperature is adequate to prevent liquids from getting to the vacuum source o For solvents and other volatile liquids use a cold trap of sufficient size and cold enough to condense vapors generated followed by a filter flask capable of collecting fluid that could be aspirated out of the cold trap o For highly reactive corrosive or toxic gases use a sorbent canister or scrubbing device capable of trapping the gas 5 13 Research Samples and Chemicals Developed in the Lab Research samples and chemicals developed in the lab samples must be managed responsibly Samples often accumulate in labs for years and are difficult to identify and dispose of and can create unsafe and non compliant conditions if not managed properly The following requirements apply to samples developed in the laboratory e All samples must be kept closed except when in use S
79. ects may only become evident after a long latency period Carcinogens are separated into two classes select carcinogens and regulated carcinogens e Corrosives Substances that cause destruction of living tissue by chemical corrosion at the site of contact and can be either acidic or caustic basic e Hazardous Substances with Toxic Effects on Specific Organs Substances that pose adverse health effects to specific organs such as the liver kidneys lungs etc e High Acute Toxicity Substances Substances that may be fatal or cause damage to target organs as the result of a single exposure or exposures of short duration Acute toxins are quantified by a substance s lethal dose 50 LD50 or lethal concentration 50 LC50 which is the lethal dose of a compound to 50 of a laboratory tested animal population e g rats rabbits over a specified time period e Irritant Substances that cause reversible inflammatory effects on living tissue by chemical action at the site of contact e Reproductive Toxins Substances that may affect the reproductive capabilities including chromosomal damage mutations and effects on fetuses teratogens e Sensitizer also called allergen A substance that causes exposed individuals to develop an allergic reaction in normal tissue after repeated exposure to the substance 17 The official version of this document will only be maintained online Chapter 2 Classes of Hazardous Chemicals 3 3 Biological Hazards
80. ecure location and lab personnel should always be present when these materials are in use 5 16 Laboratory Self Inspections REM performs laboratory inspections for various purposes e g routine building safety and compliance inspections However the Laboratory Supervisor a qualified designee should also inspect the laboratory for compliance with the requirements of the CHP at a minimum on an annual basis Lab personnel have a much greater understanding of the unique hazards and issues that are encountered in their individual lab than REM does The goal of these inspections is to identify and correct unsafe and non compliant conditions that could potentially result in an injury to lab personnel or a fine from a regulatory agency e g open hazardous waste container All deficiencies found during the inspection should be reviewed and corrected The following elements should be performed during these inspections e Housekeeping practices should be reviewed Chemicals should be stored appropriately and labeled Evidence of spills and or chemical contamination should be cleaned All glassware and equipment should be stored appropriately etc e Hazard assessments should be updated if process changes have occurred For example the lab is now performing organic synthesis and working with organometallic compounds 56 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan e Training records s
81. elf is not necessarily combustible may generally by yielding oxygen cause or contribute to the combustion of other material e Pyrophoric Substance also called Spontaneously Combustible A liquid or solid that even in small quantities and without an external ignition source can ignite after coming in contact with the air 16 The official version of this document will only be maintained online Chapter 2 Classes of Hazardous Chemicals e Self Heating Substance A liquid or solid other than a pyrophoric substance which by reaction with air and without energy supply is liable to self heat e Self Reactive Substance A liquid or solid that is liable to undergo strong exothermic thermal decomposition even without participation of oxygen air e Water Reactive Substance A liquid or solid that reacts violently with water to produce a flammable or toxic gas or other hazardous conditions 3 2 Health Hazards A chemical is a health hazard if there is statistically significant evidence based on at least one study conducted in accordance with established scientific principles that acute or chronic health effects may occur in exposed employees Each health hazard is briefly defined below Refer to Appendix B section B 2 for detailed information on each health hazard e Carcinogens Substances that cause cancer Generally they are chronically toxic substances that is they cause damage after repeated or long duration exposure and their eff
82. enerated in A central waste collection room should not be established e The waste storage volume should never exceed 55 gallons per SAA e Containers should be segregated by chemical compatibility during storage e g acids away from bases secondary containment can be used as a means of segregation e Avoid halogenated and non halogenated wastes in the same waste container e Avoid mixing incompatible waste streams in the same container e g acids with bases oxidizers with organic solvents that will potentially create an exothermic reaction in the waste container If mixing waste streams does create heat allow the container to vent and cool in a chemical fume hood before sealing to avoid over pressurization of the container as illustrated in Figure 7 2 e Collect all highly toxic reactive mercury and any exotic wastes e g dioxin compounds PCBs controlled substances separately even if they are chemically compatible with other waste streams Failing to do so can result in costly disposal fees e g mixing mercury with an organic solvent waste means that the entire waste stream must be treated as mercury waste e All spills and leaks should be cleaned up immediately e Identification of SAAs is not required by the EPA but it is recommended as a good practice 72 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management Figure 7 2 Container Failure Due to Mixing Incomp
83. ensure that all lab employees receive documented PPE training before any work with hazardous materials occurs Document PPE training using the Certification of Training form Appendix B of the Purdue University Personal Protective Equipment PPE Policy http www purdue edu rem home booklets PPEPolicy pdf or get it directly from http www purdue edu rem home forms CertT pdf Each lab employee must be trained to know at least the following e When PPE is necessary e What PPE is necessary e How to properly don doff adjust and wear PPE e The limitations of the PPE e The proper care maintenance and useful life of PPE Each affected employee must demonstrate an understanding of the training provided and the ability to use the PPE properly before performing any work requiring the use of PPE 9 3 SOP Training As discussed in Chapter 4 of the CHP SOPs are written instructions that detail the steps that will be performed during a given procedure and include information about potential hazards and how these hazards will be mitigated SOPs must be developed for all high hazard tasks that are performed in the lab which is defined as work with e Explosives e Water reactive pyrophoric self heating or self reactive chemicals e Particularly hazardous substances which includes carcinogens reproductive toxins and acutely toxic substances e Compressed gases e Work involving more than 1 liter of flammable liquids flammable solids
84. ent is exposed the device should be either discarded or repaired before it is used again e The external cases of all variable autotransformers have perforations for cooling by ventilation and therefore should be located where water and other chemicals cannot be spilled onto them and where they will not be exposed to flammable liquids or vapors e Fail safe devices can prevent fires or explosions that may arise if the temperature of a reaction increases significantly because of a change in line voltage the accidental loss of reaction solvent or loss of cooling Some devices will turn off the electric power if the temperature of the heating device exceeds some preset limit or if the flow of cooling water through a condenser is stopped owing to the loss of water pressure or loosening of the water supply hose to a condenser 5 12 4 Distillation and Solvent Purification Systems The process of thermal solvent distillation is inherently dangerous If not handled properly fire explosion and or personnel exposure can result A few common chemicals distilled in laboratories include tetrahydrofuran methylene chloride diethyl ether toluene dimethylformamide benzene and hexanes The guidelines below should be followed while thermal distillation of organic solvents is conducted in the lab e The thermal solvent distillation system should be installed inside of a chemical fume hood if possible e Ensure that all heat generating equipment has
85. epartments engaged in work with hazardous chemicals or hazardous operations that are not sufficiently covered by the CHP must customize this document by adding appropriate sections in the form of standard operating procedures SOPs hazard assessments and any other written lab specific operating procedures that address the hazards and how to mitigate risks The following instructions detail how this CHP template should be used and customized by each laboratory e Review this template CHP provided by REM e Insert your lab specific standard operating procedures SOPs into your customized CHP under Tab 1 located in the back of the CHP document More details regarding SOPs can be found in Chapter 4 of the CHP e Insert all other documented lab specific rules requirements and procedures e g equipment protocols internal lab inspections etc under Tab 2 e Insert your lab specific hazard assessments under Tab 3 More details regarding hazard assessments can be found in Chapter 6 of the CHP e Review update if necessary and retrain all employees on the lab specific CHP at least annually 1 4 Employee Rights and Responsibilities As part of the OSHA Laboratory Standard employees and other personnel who work in laboratories have the right to be informed about the potential hazards of the chemicals in their 3 The official version of this document will only be maintained online Chapter 1 Introduction work areas and to be properly
86. er is chipped or broken properly dispose of it Always wear appropriate PPE when working with glassware and varying temperatures Always wear safety glasses 5 12 6 High Pressure Systems Working with high pressure systems in a laboratory can result in over pressurization explosion and the possible hazards of flying glass chemical exposure and fire All high pressure systems must be set up and operated with careful consideration of potential risks The following procedures should be followed when working with high pressure systems in the laboratory High pressure operations should be performed only in pressure vessels appropriately selected for the operation properly labeled and installed and protected by pressure relief control devices Vessels connecting hoses and any apparatus must be strong enough to withstand the stresses encountered at the intended operating temperatures and pressures and must not corrode or otherwise react when in contact with the materials it contains All pressure equipment should be visually inspected before each use 49 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 12 7 Vacuum Systems Vacuum work can result in an implosion and the possible hazards of flying glass splattering chemicals and fire All vacuum operations must be set up and operated with careful consideration of the potential risks The following guidelines shoul
87. er repeated exposure to the substance Examples of sensitizers include diazomethane chromium nickel formaldehyde isocyanates arylhydrazines benzylic and allylic halides and many phenol derivatives Sensitizer exposure can lead to all of the symptoms associated with allergic reactions or can increase an individual s existing allergies 91 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions B 2 3 Corrosives Corrosive substances cause destruction of living tissue by chemical corrosion at the site of contact and can be either acidic or caustic basic Major classes of corrosive substances include e Strong acids such as sulfuric nitric hydrochloric and hydrofluoric acids e Strong bases such as sodium hydroxide potassium hydroxide and a X ammonium hydroxide e Dehydrating agents such sulfuric acid sodium hydroxide phosphorus pentoxide and calcium oxide e Oxidizing agents such as hydrogen peroxide chlorine and bromine Chapter 5 7 6 of the CHP details corrosives storage requirements B 2 4 Hazardous Substances with Toxic Effects on Specific Organs Substances with toxic effects on specific organs include e Hepatotoxins which are substances that produce liver damage such as nitrosamines and carbon tetrachloride e Nephrotoxins which are substances that cause damage to the kidneys such as certain halogenated hydrocarbons e Neurotoxins which are substances tha
88. eresssrerseeee 84 95 REM Researcher S GUII Osni aea AA EA AAEE 84 Appendix A CHP Awareness Certification FOrim sessesessesesssoecsscessecesescecesoecesoecessecesseceeee 85 iii The official version of this document will only be maintained online Table of Contents Appendix B OSHA Hazard Class Defimitions csccscssscsccscsccccccscsccccccscsceccccecsceseccecscecess 87 Bed PhysicakHazardSram uniia naaa a aa cee canseatauue a a aa 88 BALL FammnableLliguid Seisean tcuaa diac tae GctuasadiecnnOnitaaah ereneaeceeatanes 88 Bla sl daligt 8 oles 10 6 Once ate on nen R T T Re ee 88 BAAS Gases Under Pressure enson eideensnedeancnerbatcanesieanen tenes 89 B 1 4 Pyrophoric Self Heating and Self Reactive Materials ccccccssecccseseeeeseseneeeees 89 BLS WaterReactive Matenale seeria r N A ixacstebhedatimiciakeuetaaes 90 BL OAZE a AEN A E E A te ates 90 BiZ ORE ANIC Peroxide S crisa a a a a eo teen 90 BLE cb 4 9 Jc IVES aa ree Rn aR a E 91 BeZ Health Hazards sec cicestes cesses can ee tuou Sc Gadetede nsta E E E N 91 BL APE a E A AE 91 BAA SOUS EI ZOMG oi E T E a a R cle S due 91 Ba COMOSIVES niiae aran T T deena eaeaeoaks 92 B 2 4 Hazardous Substances with Toxic Effects on Specific Organs cccccsssececesseeeeesees 92 B 2 5 Particularly Hazardous Substances efcc cedtecsarcnssecsactdcncta sethoousdtecncinsecnissaatasncdesacheanetees 92 B291 C areno EN e a E E chew en aap eenaen ee teatereat 93
89. erse any part of your body into a cryogenic liquid 5 10 Nanoparticle Safety The American Society of Testing and Materials ASTM Committee on Nanotechnology has defined a nanoparticle as a particle with lengths in two or three dimensions between 1 and 100 nanometers nm Nanoparticles can be composed of many different base materials and may be of different shapes including nanotubes nanowires and crystalline structures such as fullerenes and quantum dots Nanoparticles present a unique challenge from an occupational health perspective as there is a limited amount of toxicological data currently available for review However some studies have shown that existing exposure control technologies have been effective in reducing exposure to nanoparticles Refer to the REM webpage for detailed procedures and guidance regarding the safe handling of nanoparticle http www purdue edu rem home booklets nanopolicy pdf 5 11 Sharps Handling Safety Sharps are defined as items capable of puncturing cutting or abrading the skin such as glass or plastic pipettes broken glass test tubes petri dishes razor blades needles and syringes with needles Sharps are often contaminated with hazardous chemicals and or infectious agents so multiple hazards are often encountered Employees that routinely work with sharps must be aware of the risk of being punctured or lacerated It is important for these employees to take precautions and properly handle sharps in
90. ertification Form must be completed for all lab employees This instruction and information box should remain This model Chemical Hygiene Plan is the 2014 version the most current version can be found on the Forms page at www purdue edu rem The official version of this document will only be maintained online Table of Contents Table of Contents CHP DOCUMENT ACFONVINIS Listesine e EN eel eda E 1 Chapter Te INtFOGUCUION sorsi n E senses iu sounseteesvanssansbuneceaeesueaouies 2 EE PUDOSG ee eee ert ae nce UE eI ete Rr oR ee reer eee 2 E2 COP Ct coer ees ec iict N N cicoon upc tonson anne eae man oceans aeaae a anetrauseattacneaeions 2 iad CHIP ISG IMS UPUGCIOMS serinin E A eae eee 3 1 4 Employee Rights and Responsibilities sccciscscssicieisisesecinasdoieed sejeneasiachdeisancsersacdpiaes aj edessedeoes 3 1 4 1 Laboratory Supervisor Responsibilities ee eeccceeseceeeececeeececeeeceeeeeceseeecesseeeees 4 1 4 2 Laboratory Employee Responsibilities w d ssosecsevesedseaigevscresinededsdtaveasedededieadavesabasaeateiveia 5 1 4 3 Laboratory Safety Officer Responsibilities esseeseeenseensrensrensrensrresrersressrersrersrrsseees 5 1 4 4 Non Laboratory Personnel Support Staff Responsibilities eseneeeeseeseeseessreeesn 6 1 4 5 Chemical Hygiene Officer Responsibilities s nsssensesensseensseesssrressrrsseerssrerssersseereses 6 1 5 Radiological amp Environmental Management Department sssssessseerssee
91. es Non emergency chemical spills are generally defined as less than 1 liter do not involve a highly toxic or reactive material do not present a significant fire or environmental hazard and are not in a public area such as a hallway These spills can be cleaned up by properly trained lab personnel using conventional lab PPE e g safety glasses goggles lab coat gloves and the lab spill kit In general when a non emergency spill occurs in the lab the area around the spill should be isolated everyone in the lab should be made aware of the spill and the spilled material should be absorbed and collected using either pads or some other absorbent material such as oil dry or kitty litter Decontamination of the spill area should be conducted using an appropriate solvent soap and water is often the most effective Proper PPE should be worn at all times and only trained personnel should conduct the cleanup Additionally review the SDS s specifically Section 6 Accidental Release Measures to obtain chemical specific cleanup information 8 2 Emergency Chemical Spill Procedures Emergency chemical spills are generally defined as greater than 1 liter involve a highly toxic or reactive compound present an immediate fire or ai nae Ty 5 17 ia ar f t _ environmental hazard or require additional PPE e g respirator and specialized training to properly a cleanup The following procedures should be followed in the even
92. es are required Skin and Body Protection Laboratory coats must be worn and be appropriately sized for the individual and buttoned to their full length flame resistant lab coats must be worn if handling flammable gases such as hydrogen Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel must also wear full length pants or equivalent and close toed shoes Full length pants and close toed shoes must be worn at all times by all individuals that are occupying the laboratory area The area of skin between the shoe and ankle must not be exposed Hygiene Measures Wash thoroughly and immediately after handling Remove any contaminated clothing and wash before reuse Section 6 Engineering Controls Use of compressed gases should be conducted in a properly functioning chemical fume hood whenever possible The chemical fume hood must be approved and certified by REM and have a face velocity between 85 125 feet Tab 2 Lab Specific Protocols Requirements Rules per minute Contact REM for information regarding specific handling requirements when work with toxic highly toxic corrosive and reactive gases Generally these of gases need to be stored and used with local exhaust ventilation e g fume hood or gas cylinder cabinet Section 7 First Aid Procedures If inhaled Move into the fresh air immediately Consult a physician If not breathing give artificial respiration
93. f this document will only be maintained online Chapter 2 Chemical Classification Systems Chapter 2 Chemical Classification Systems Chemical classification systems are designed to communicate hazards The three most widely used classification systems are the OSHA Globally Harmonized System for Classifying and Labeling Chemicals recently adopted and implemented under the OSHA Hazard Communication Standard the National Fire Protection Association NFPA system of classifying the severity of hazards and the Department of Transportation DOT hazard classes These classification systems are used by chemical manufacturers when creating safety data sheets and chemical labels therefore it is important that Purdue lab employees understand the basic elements of each classification system 2 1 Globally Harmonized System for Classifying Chemicals The Globally Harmonized System GHS is a world wide system adopted by OSHA for standardizing and harmonizing the classification and labeling of chemicals The objectives of the GHS are to e Define health physical and environmental hazards of chemicals e Create classification processes that use available data on chemicals for comparison with the defined hazard criteria numerical hazard classification is based ona1 5 scale 1 being the most hazardous and 5 being the least hazardous and e Communicate hazard information as well as protective measures on labels and Safety Data Sheet SDS former
94. flasks vials and test tubes The label should be placed on the container not the cap to avoid accidental mislabeling e Immediately replace labels that have fallen off or that are deteriorated e Label containers using chemical names Do not use abbreviations structure or formulae e Archived research samples are often stored in boxes containing hundreds of small vials Label the outside of the box with the chemical constituents paying special attention to regulated materials such as radioactive material organic solvents heavy metals and other toxics If the samples are nonhazardous label them as such e Submit frequent Hazardous Materials Pickup Request Forms to reduce the amount of chemicals in your laboratory e Employees should dispose of all of their waste before leaving graduating from Purdue The lab and or department should come up with a system to ensure that all faculty staff and students properly dispose of hazardous waste including unwanted research samples before employees leave 77 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management 7 7 Sink and Trash Disposal No chemical waste should be poured down the drain or discarded in the trash unless it is certain that doing so does not violate hazardous waste regulations or the West Lafayette wastewater treatment plant s discharge requirements In order to ensure improper disposal does not occur the detailed instruc
95. fore any work in a glove box occurs All trained personnel must understand the design features and limitations of a glove box before use The training must include detailed instruction on elements such as the ventilation and vacuum controls that maintain a pressure differential between the glove box and outside atmosphere atmospheric controls e g controlling oxygen concentrations and moisture etc e Prior to use a visual glove inspection must be performed Changing of a glove must be documented date manufacturer model of glove and person performing change Gloves should not be used until they fail they should be changed according to the glove box manufacturer s recommendations or whenever necessary e Plugging ports that are never or infrequently used is recommended A properly plugged port should have a stub glove and a glove port cap installed e Chemical resistant gloves e g disposable nitrile gloves should be used under the glove box gloves to protect from contamination e The glove box pressure must be checked every day before use and immediately after gloves are changed The pressure check must be documented 22 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls e Keep sharps in an approved container while in the glove box e Do not work in the glove box unless the lighting is working e Follow all safe work practices for using and handling compressed gas that m
96. g REM s laboratory ergonomics program http www purdue edu rem safety ergo htm 5 18 Laboratory Electrical Safety 5 18 1 Training Laboratory employees shall be trained to understand the specific hazards associated with electrical energy See the written Electrical Safety Program on the REM website for more detailed information http www purdue edu rem home booklets elsp pdf Employees who need access to operate circuit breakers and fused switches in electrical panels may require additional training to be designated by their supervisor as qualified for the task 57 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 18 2 Portable Electrical Equipment and Extension Cords The following requirements apply to the use of cord and plug connected equipment and flexible cord sets extension cords e Extension cords may only be used to provide temporary power and must be used with Ground Fault Circuit Interrupter GFCI protection during maintenance and construction activities and in damp or wet locations e Portable cord and plug connected equipment and extension cords must be visually inspected before use for external defects such as loose parts deformed and missing pins or damage to outer jacket or insulation and for possible internal damage such as pinched or crushed outer jacket Any defective cord or cord and plug connected equipment must be removed from service and no
97. h goggles a face shield cryogenic safe gloves long sleeves long pants and closed toe shoes e Cryogenic liquids will vent boil off from their storage containers as part of normal operation Containers are typically of a vacuum jacketed design to minimize heat loss Excessive venting and or an isolated ice build up on the vessel walls may indicate a fault in the vessel s integrity or a problem in the process line A leaky container should be removed from service and taken to a safe well ventilated area immediately e All systems components piping valves etc must be designed to withstand extreme temperatures e Pressure relief valves must be in place in systems and piping to prevent pressure build up Any system section that could be valved off while containing cryogenic liquid must 44 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan have a pressure relief valve The pressure relief valve relief ports must be positioned to face toward a Safe location e Transfer operations involving open cryogenic containers such as Dewars must be done slowly while wearing all required PPE Care must be taken not to contact non insulated pipes and system components e Open transfers will be allowed only in well ventilated areas e Do not use a funnel while transferring cryogenic liquids e Use tongs or other similar devices to immerse and remove objects from cryogenic liquids never imm
98. hould be updated and documented if new lab personnel have not yet been trained or if any processes have changed e Excess or outdated chemicals should be properly disposed of by REM e Safety supplies such as PPE and spill containment equipment should be replenished if necessary A recommended template to use during laboratory self inspections is the ISP Self Audit Checklist which can be found on the REM website https www purdue edu rem home forms ispcheck pdf 5 17 Laboratory Ergonomics Many tasks in laboratories require repetitive motions which may lead to cumulative trauma injuries of the body these effects can be long term Tasks like pipetting weighing multiple Samples standing at the bench or hood and using microscopes for long periods of time can cause physical stress Even time compiling data at a computer poses potential physical problems Ergonomics is the study of interaction of the human body with the work environment Ergonomics strives to fit the job to the body through proper body positioning posture movement tools workplace layout and design Parts of the body commonly affected by poor ergonomics include neck shoulders back hands wrists elbows legs and feet REM has resources available to improve ergonomic conditions and help reduce cumulative trauma injuries to laboratory workers Often simple adjustments are all that is required to improve conditions Refer to the REM webpage for detailed information regardin
99. ical inventory Taking a routine chemical inventory can reduce the number of unknown chemicals and the tendency to stockpile chemicals REM recommends that all laboratories take a chemical inventory at least annually Depending on the type of chemicals being used and stored in a laboratory REM may require that a chemical inventory be prepared for a room work unit or department e g Department of Homeland Security Chemical Facility Anti Terrorism Standards Inventory on a routine basis 5 4 Safety Data Sheets The SDS provides comprehensive information that is imperative for the safe handling of hazardous chemicals Carefully read the label and SDS and make sure that you understand the information provided in this document before using a chemical In some cases it may be necessary to do additional research The Laboratory Supervisor should be consulted if necessary It is important that all lab employees have access to SDS for all hazardous chemicals that are stored in the lab Access can mean storing hard copies of SDS in the lab or some other easily accessible location e g departmental main office or can mean storing electronically by a means that is also accessible to all lab personnel e g shared network drive To obtain a copy of a SDS contact the chemical manufacturer or REM at 765 49 46371 Many manufacturers SDS can be found online at REM s SDS webpage or other websites such as Siri MSDS Index The links to these resources ar
100. iency as it removes heat from the storage container Nitrogen is fundamentally cheap as it makes up 82 of the air we breathe the expense come in the electrically intensive cooling process and the vacuum storage apparatus which generally must be stainless steel It is also quite heavy having similar density to water when liquified 1L of liquid N will expand to about 800 of gas at room temperature hence the need for pressure relief in storage Tips When you use gas or liquid the pressure will drop for a while If it drops too low for some apparatus you can use the pressure builder to bring the pressure back up If you leave the pressure builder open it will bring the pressure up to 220psi and boil off a lot of the liquid The whisper valve relieves pressure at 180 psi The tank will leak slowly all the time This is how it stays cold The whisper valve leaks out a steady flow of gas at 180 psi Safety Contents under pressure loud surprising and slightly dangerous release of gas flows possible Contents Cold Frostburns are possible It will generally boil off smooth skin w o damage but will adhere to hair and cause local burns Getting liquid nitrogen in your eyes could be dangerous Tab 2 Lab Specific Protocols Requirements Rules Contents can axsifiate in sufficient volume If sufficient nitrogen were released in a sealed environment if could displace the 18 2 Oxygen to dangerously low levels In non recirculating chemistry la
101. ific Hazard 4 Deadly 4 FP lt 73 F 4 May Detonate ACID Acid 3 Extreme Danger 3 FP lt 100 F 3 Shock Heat May ALK Alkali 2 Hazardous 2 100 F lt FP lt 200 F Detonate COR Corrosive 1 Slightly Hazardous 1 FP gt 200 F 2 Violent Chemical W No Water 0 Normal Material 0 Will Not Burn Change 4 1 Unstable if Heated FP Flash Point 0 Stable Radioactive Figure 2 4 NFPA Hazard Rating System 2 3 Department of Transportation Hazard Classes The DOT regulates the transportation of all hazardous materials in the United States and defines a hazardous material as any substance that has been determined to be capable of posing an unreasonable risk to health safety or property when transported in commerce There are several methods that can be employed to determine whether a chemical is hazardous for transport a few of which included e Reviewing the DOT Hazardous Materials Table 49 CFR 172 101 e Reviewing the SDS specifically Section 2 Hazardous Identification and Section 14 Transport Considerations for the chemical being shipped as detailed above in Section 2 1 1 of the CHP 14 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems e Reviewing the chemical label and looking for hazard information detailed above in Section 2 1 2 of the CHP and e Understanding the chemical and physical properties of the chemical All ha
102. ill only be maintained online Tab 2 Lab Specific Protocols Requirements Rules Tab 1 Specific Standard Operating Procedures Liquid Nitrogen Base Bath Compressed gasses Tab 2 Lab Specific Protocols Requirements Rules Standard Operating Procedure Liquid Nitrogen This is an SOP template and is not complete until 1 lab specific information is entered into the box below 2 lab specific protocol procedure is added to the protocol procedure section and 3 SOP has been signed and dated by the PI and relevant lab personnel Print a copy and insert into your Lab Specific Chemical Hygiene Plan Chapter 10 Section 1 Lab Specific Information Department Chemistry Date SOP was written 20141013 Date SOP was approved by PI lab supervisor 20141013 Principal Investigator Dr Shelley Claridge Internal Lab Safety Coordinator Lab Manager David McMillan Lab Phone 4 9134 Office Phone 4 6070 Emergency Contact David McMillan 4 9134 Name and Phone Number Location s covered by this SOP BRWN 4144 and 4144A Building Room Number Section 2 Type of SOP L Process lt I Hazardous Chemical Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS 7727 37 9 GHS Classification Gas under pressure cryogenic liquid Tab 2 Lab Specific Protocols Requirements Rules Molecular Formula N2 Form physical state Liquid Boiling Point 196 C 320
103. in the REM managed medical surveillance program when required and 10 Inform the Laboratory Supervisor of any work modifications ordered by a physician as a result of medical surveillance occupational injury or chemical exposure 1 4 3 Laboratory Safety Officer Responsibilities Very often it is not practical for the Laboratory Supervisor PI to be present in the lab on daily basis to ensure that safe and compliant practices are being carried out by all lab staff For this reason it is highly recommended that each PI establish a Laboratory Safety Officer to manage the daily operations of the lab s safety program The PI should empower the Laboratory Safety Officer to make decisions on daily operations involving safety and compliance including the authority to instruct other lab personnel to follow all safety 5 The official version of this document will only be maintained online Chapter 1 Introduction procedures e g PPE use hazardous waste procedures etc This person should be familiar with how the lab operates and have demonstrated lab safety experience e g senior graduate student post doc lab manager Having a Laboratory Safety Officer in each lab provides many benefits such as e Other lab personnel know who to contact with questions about daily operations involving safety and compliance e Empowers someone other than the PI to enforce lab safety rules e Provides consistency within the respective academic department
104. l acutely toxic materials in a cabinet dedicated only for acutely toxic materials Signs should be posted to indicate their presence and unique hazards 39 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan e The amount of acutely toxic material stored in the lab should be kept at a minimum Any expired or unnecessary materials must be properly disposed of as hazardous waste e All acutely toxic materials should be clearly labeled with the original manufacturer s label which should have the chemical name hazard labels and pictograms The label should not be defaced in any way 5 7 6 Corrosive Materials Storage Corrosive materials are defined as substances that cause destruction of living tissue by chemical corrosion at the site of contact and can be either acidic or basic caustic The best storage method for corrosive materials is inside of a corrosive storage cabinet or lab cabinet where acids and bases are segregated at all times Acids must also be segregated from chemicals where a toxic gas would be generated upon contact with an acid e g reactive cyanide compounds Organic acids e g acetic acid formic acid must be stored away from oxidizing acids e g nitric acid perchloric acid as these types of acids are incompatible with each other Segregation can be achieved either by physical distance preferred method or by secondary containment as shown in Figure 5 6 A
105. l kits can either be purchased from a vendor or created by lab personnel but each spill kit should be equipped to handle small spills of the most common hazards in the laboratory The kit should be equipped with response and cleanup materials such as e Absorbent materials such as pads booms oil dry or kitty litter booms or pillows e Neutralizing agents e g Neutrasorb for acids and or bases if high volume of acids and or bases are stored in the laboratory e Containers such as drums buckets and or bags to containerize spilled material and contaminate debris generated during the cleanup process e PPE such as gloves safety glasses and or goggles lab coat or apron chemical resistant booties e Caution tape or some other means to warn people of the spill 81 The official version of this document will only be maintained online Chapter 9 Training Chapter 9 Training Effective training is crucial to a successful laboratory safety program Laboratory Supervisors must actively participate in the training process to ensure that all lab employees are effectively trained before any work with hazardous materials occurs This chapter details the minimum training requirements for all Purdue laboratories It should be noted that depending on the type of research being conducted and associated hazards there may be additional training requirements that are not detailed in this chapter For more information contact REM at 765 49 637
106. laundry services as needed to maintain the hygiene of laboratory coats They may not be cleaned by staff members at private residences or public laundry facilities Alternatives to laundering lab coats include routinely purchasing new lab coats for employees to replace contaminated lab coats or using disposable lab coats 6 4 7 Foot Protection Closed toe shoes must be worn at all times when in the laboratory open toe shoes and or sandals are not permitted in any circumstance Each affected employee must wear protective footwear when working in areas where there is a high risk of objects falling on or rolling across the foot piercing the sole and where the feet are exposed to electrical or chemical hazards If there is a high risk of chemical contamination to the foot e g cleaning up a chemical spill on the floor then chemical resistant booties may need to worn as well Minimum PPE Requirements for Support Staff and Visitors Support staff e g custodians maintenance workers and visitors often must enter laboratories to perform routine tasks such as maintenance or take a tour of the lab These individuals are present in the laboratory but are not performing work with or directly adjacent to any work with hazardous chemicals To be present in the laboratory the minimum PPE requirements include safety glasses long pants and closed toe shoe If additional PPE is required or if other unique safety requirements must be fol
107. lf reacting compounds and water reactives Many reactive materials are also toxic and are dissolved or immersed in a flammable solvent e g lithium alkyl compounds dissolved in diethyl ether sodium metal immersed in mineral oil Other common hazards often associated with reactive chemicals include corrosivity teratogenicity or organic peroxide formation The following guidelines for storing reactive materials must be followed in all laboratories The amount of reactive materials stored in the lab must be kept to a minimum Any expired or unnecessary reactive materials must be properly disposed of as hazardous waste All reactive materials must be clearly labeled with the original manufacturer s label which should have the chemical name hazard labels and pictograms The label should not be defaced in any way All reactive materials should be placed into secondary containment as a best management practice Suitable storage locations for reactive materials include inert gas filled desiccators or glove boxes flammable storage cabinets that do not contain aqueous or other incompatible chemicals or intrinsically safe refrigerators or freezers that also do not contain aqueous or other incompatible chemicals If possible store all reactive chemicals in a small flammable cabinet dedicated only for reactives Signs should be posted to indicate their presence and unique hazards as shown in Figure 5 5 38 The official version of this doc
108. lowed it is the lab personnel s responsibility to notify support staff and or visitors of the additional requirements PPE Training Requirements Laboratory Supervisors must ensure that all employees receive PPE training before any work with hazardous materials occurs This training must be documented Document PPE training 67 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy using Certification of Training form Appendix B of the Purdue University Personal Protective Equipment PPE Policy http www purdue edu rem home booklets PPEPolicy pdf or access it directly from http www purdue edu rem home forms CertT pdf Each lab employee must be trained to know at least the following e When PPE is necessary e What PPE is necessary e How to properly don doff adjust and wear PPE e The limitations of the PPE and e The proper care maintenance and useful life of PPE Each affected employee must demonstrate an understanding of the training provided and the ability to use the PPE properly before performing any work requiring the use of PPE When the supervisor has reason to believe that an affected employee who has already been trained does not have the understanding and skill required e g employee is seen handling hazardous materials without wearing proper PPE then the supervisor must ensure the employee is retrained 6 7 Injuries Illnesses and Medical Examina
109. ls across campus via foot If a large volume of chemicals needs to be moved such as an entire lab move contact REM 765 49 40121 for further assistance The following procedures must be followed when moving chemicals on campus by way of foot e PPE must be worn when handling potentially contaminated surfaces During the time which the chemicals are moved on campus via foot PPE may not be necessary or even appropriate e g employees should not wear chemical resistant gloves in public areas However appropriate PPE and spill containment equipment should be brought along in the event of a spill or incident e Purdue Stores stock room personnel shall not dispense or sell chemicals in breakable containers of any size unless the customer has an approved transport container in which to place the chemical for transporting before leaving the Stock Room Chemical requisitioners may purchase a transport container from Purdue Stores Approved transport container means a commercially available bottle carrier made of rubber metal or plastic with carrying handle s which is large enough to hold the contents of the container if broken in transit Carrier lids Figure 5 10 Chemical Bottle Carrier or covers are recommended but not required Rubber or plastic should be used for acids alkalis and metal rubber or plastic for organic solvents An example of a bottle carrier is illustrated in Figure 5 10 e Laboratory carts used to transport
110. ly known as Material Safety Data Sheets MSDS 2 1 1 Safety Data Sheets The SDS provides comprehensive information that is imperative for the safe handling of hazardous chemicals Laboratory personnel should use the SDS as a resource to obtain information about hazards and safety precautions SDSs cannot provide information for hazards in all circumstances However the SDS information enables the employer to develop an active program of worker protection measures such as training on hazard mitigation Chemical manufacturers are required to use a standard format when developing SDSs The SDS will contain 16 headings which are illustrated in Figure 2 1 9 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems Identification of the substance or l l 1 Physical and chemical properties mixture and of supplier Hazards Identification Stability and reactivity Composition information on NDES l P 11 Toxicological information ingredients pa First aid measures Ecological information Firefighting measures Disposal considerations Accidental release measures Transport considerations 7 Handling and storage Regulatory information Exposure controls personal A 16 Other information protection Figure 2 1 GHS Required Sections of a Safety Data Sheet 2 1 2 Chemical Labeling The GHS standardized label elements which are not subject to variation and must appear o
111. ment used for laboratory operations Because stirring and mixing devices especially stirring motors and magnetic stirrers are often operated for fairly long periods without constant attention the consequences of stirrer failure electrical overload or blockage of the motion of the stirring impeller should be considered 5 12 3 Heating Devices Laboratories commonly use heating devices such as ovens hot plates heating mantles oil baths salt baths sand baths air baths hot tube furnaces hot air guns and microwave ovens Steam heated devices are generally preferred whenever temperatures of 100 C or less are required because they do not present shock or spark risks and can be left unattended with assurance that their temperature will never exceed 100 C Ensure the supply of water for steam generation is sufficient prior to leaving the reaction for any extended period of time A number of general precautions need to be taken when working with heating devices in the laboratory When working with heating devices consider the following 47 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan e The actual heating element in any laboratory heating device should be enclosed in such a fashion as to prevent a laboratory worker or any metallic conductor from accidentally touching the wire carrying the electric current e fa heating device becomes so worn or damaged that its heating elem
112. ment will only be maintained online Chapter 6 Laboratory PPE Policy regulated substance for which there are exposure monitoring and medical surveillance requirements medical surveillance shall be established for the affected employee as prescribed by the particular standard and Whenever an event takes place in the work area such as a spill leak explosion or other occurrence resulting in the likelihood of a hazardous exposure the affected employee Shall be provided an opportunity for a medical examination All medical examinations must be performed by or under the direct supervision of a licensed medical care provider and must be provided without cost to the employee 69 The official version of this document will only be maintained online Chapter 7 Hazardous Waste Management Chapter 7 Hazardous Waste Management 7 1 Introduction Hazardous waste is generally defined as waste that is dangerous or potentially harmful to human health or the environment Hazardous waste regulations are strictly enforced by both the Environmental Protection Agency EPA and the Indiana Department of Environmental Management The laboratory supervisor is responsible for managing the hazardous waste program in a safe and compliant manner No chemical waste should be poured down the drain or discarded in the trash unless it is certain that doing so does not violate hazardous waste regulations or the West Lafayette wastewater treatment plant s requirements
113. n the chemical label contain the following elements e Symbols hazard pictograms are used to convey health physical and environmental hazard information assigned to a GHS hazard class and category e Signal Words such as Danger for more severe hazards or Warning for less severe hazards are used to emphasize hazards and indicate the relative level of severity of the hazard assigned to a GHS hazard class and category e Hazard statements e g Danger Extremely Flammable Liquid and Vapor are Standard phrases assigned to a hazard class and category that describe the nature of the hazard and e Precautionary statements are recommended measures that should be taken to minimize or prevent adverse effects resulting from exposure to the hazardous chemical GHS also standardizes the hazard pictograms that are to be used on all hazard labels and SDSs There are 9 pictograms that represent several defined hazards and include the harmonized hazard symbols which are intended to convey specific information about each hazard Figure 2 2 illustrates these GHS hazard pictograms 10 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems Carcinogen Respiratory Sensitizer Reproductive Toxicity Target Organ Toxicity Mutagenicity Flammable Pyrophoric Self Irritant Dermal Sensitizer Heating Emits Flammable Gas Acute Toxicity harmful Organic Peroxide Narcotic Effe
114. ncipal Investigator Dr Shelley Claridge Internal Lab Safety Coordinator Lab Manager David McMillan Lab Phone 4 9134 Office Phone 4 6070 Emergency Contact David McMillan 49134 Name and Phone Number Location s covered by this SOP 4144 4144A Building Room Number Section 2 Type of SOP L Process Hazardous Chemical Hazardous Class Section 3 Physical Chemical Properties and Uses Physical Chemical Properties CAS N A GHS Classification Gases under pressure compressed gas depending on the type of compressed gas other hazards such as toxicity and or flammability often apply as well Tab 2 Lab Specific Protocols Requirements Rules Molecular Formula N A Form physical state Gas Color N A Boiling Point N A Flash Point N A Lower Explosive Limit N A Upper Explosive Limit N A Relative Vapor Density N A Section 4 Potential Hazards Contains gas under pressure may explode if heated Protect from sunlight Gases may displace oxygen and present an asphyxiation hazard Many gases present other hazards make sure that all of the potential hazards are understood before handling any chemical Section 5 Personal Protective Equipment PPE Respirator Protection If compressed gases are being used outside of a chemical fume hood respiratory protection may be required If this activity is absolutely necessary contact REM so a respiratory protection analysis can be performed Res
115. nd the glove manufacturer s glove chart should be consulted to select the most appropriate glove Do not purchase gloves from a manufacturer that does not provide an adequate glove chart It is recommended that each lab purchase a general purpose disposable nitrile glove nitrile gloves are typically more versatile and provide resistance to a wider range of chemicals than latex gloves do with a minimum of a 4 mil thickness that is suitable for general chemical handling When handling chemicals with harmful temperature extremes such as liquid nitrogen or autoclaves appropriate protection such as cryogenic gloves or heat resistant gloves must be worn The volume of hazardous chemical being handled should be considered as well For example if working with a small volume of a sodium hydroxide solution disposable chemical resistant gloves provide adequate protection But if working with a large volume of sodium hydroxide as with a base bath for instance a more durable glove such as a butyl rubber should be selected to provide adequate protection Chemical resistant gloves must not be worn outside of the laboratory e g hallways elevators offices to avoid contamination of public areas Gloves should also be removed prior to handling any equipment that could likely result in cross contamination e g water fountains telephones computer work stations Disposable gloves must never be reused 6 4 6 Body Protection Each affected employee mu
116. ng that hazard assessments are performed and the certification s is written signed dated and readily available or posted in each location The Laboratory Supervisor is also responsible for ensuring that all lab personnel receive documented training on applicable hazard assessments The certification of hazard assessment should be reviewed at least annually and updated any time a process is modified or when a new task which presents a hazard is introduced into the lab 61 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy Hazard assessments can be organized using three formats by individual task e g pipetting hazardous liquids by location e g Chemistry Laboratory Room 1250 or by job title e g Chemistry Lab Technician Any of these formats is acceptable and often will be used in conjunction with each other to provide the safest laboratory work environment possible for employees The following subsections describe each hazard assessment format in more detail 6 3 1 Task Evaluation Hazard Assessment Task evaluation hazard assessments should be conducted for specific tasks such as preparing dilute hydrochloric acid solutions or an ozonolysis reaction and workup These types of hazard assessments should be written in a very detailed manner The following describes the steps that should be taken to perform a task evaluation hazard assessment e Describe the task e List hazards a
117. ning to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures e The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer e The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year have read and understand the content of this SOP Tab 2 Lab Specific Protocols Requirements Rules 5 m Click here to enter text Click here to enter D nn nna Click here to enter text Click here to enter D enna Click here to enter text Click here to enter a Fr nl Click here to enter text Click here to enter D nine Click here to enter text Click here to enter G nina Click here to enter text Click here to enter a R Click here to enter text Click here to enter i onan Click here to enter text Click here to enter i enna Click here to enter text Click here to enter a nina Click here to enter text Click here to enter an nn rani Click here to enter text Click here to enter i enna Click here to enter text Click here to enter a nanan Click here to enter text Click here to enter perme free Click here to enter text Click here to enter i nine Tab 2 Lab Specific Protocols Requirements Rules Standard Operating Procedure Base Baths Alcoholic
118. novation maintenance repair or experimental work The following additional requirements apply e Ground fault protection e g GFCI must be provided on all temporary wiring circuits including extension cords used for construction or maintenance activities e Ingeneral all equipment and tools connected by cord and plug must be grounded Listed or labeled double insulated tools and appliances need not be grounded e Receptacles must be of the grounding type e Flexible cords and cables must be of an approved type and suitable for the location and intended use They may not be used as a substitute for the fixed wiring where run through holes in walls ceilings or floors where run through doorways windows or similar openings where attached to building surfaces or where concealed behind building walls ceilings floors rugs or carpeting e Suitable disconnecting switches or plug connects must be installed to permit the disconnection of all ungrounded conductors of each temporary circuit e Lamps for general illumination must be protected from accidental contact or damage either by elevating the fixture above 8 feet above the floor or other working surface or by providing a suitable guard Hand lamps supplied by flexible cord must be equipped with a handle of molded composition or other approved material and must be equipped with a substantial bulb guard 59 The official version of this document will only be maintained online Chapter
119. nt in the refrigerator and freezer compartments If plastic trays are not available liquid chemicals should be placed in secondary containers to contain spills e Remember that power outages and technology failures can cause internal temperatures to rise which can impact chemical contents Be aware of unusual odors vapors etc when opening the refrigerator or freezer e An inventory should be posted on the refrigerator door e Chemical refrigerator or freezers should be located away from laboratory exits e Refrigerators and freezers should be cleaned out and manually defrosted as necessary e When defrosting a freezer consideration should be taken regarding potential chemical contamination of the water If the water draining from a defrosted refrigerator may be chemically contaminated contact REM at 765 49 40121 for further assistance Compressed Gas Cylinder Safety Compressed gas storage requirements are discussed above in Section 5 7 3 However there are additional important safety requirements for use of compressed gases in laboratories detailed below Gas cylinder connections and fittings must be inspected frequently for deterioration Never use a leaking corroded or damaged cylinder and never refill compressed gas cylinders When stopping a leak between cylinder and regulator always close the valve before tightening the union nut The regulator must be replaced with a safety cap when the cylinder is not in use Th
120. nter a date Click here to enter text Click here to enter a date Click here to enter text Click here to enter a date Click here to enter text Click here to enter a date Click here to enter text Click here to enter a date Tab 2 Lab Specific Protocols Requirements Rules Tab 2 Lab Specific Protocols Requirements Rules Tab 2 Lab Specific Protocols Requirements Rules Hydrogen Flame annealing Nitrogen dewar exchange Tab 2 Lab Specific Protocols Requirements Rules Hydrogen Flame Annealing Agilent Technologies Au 111 substrates are high purity gold epitaxially grown onto green mica in a high vacuum The resulting gold surface is 1500 angstroms thick and composed of flat Au 111 terraces up to 280 000 nm 2 Hydrogen flame annealing produces contaminant free reconstructed Au 111 surfaces Safety considerations Hydrogen is a flammable gas that burns with oxygen to form water The flame is bright and colorless Make sure that all of the safety requirements are met before starting Tools and Materials required 1 Pressurized H 2 gas cylinder Single stage high purity flow regulator Anti backflash arrester Square quartz or metal plate plate Glass slide Goggles or safety glasses Torch Quartz tube narrows to a fine tip with inner diameter less than 0 25 mm oe UE Ta Procedure A clean environment is required for proper annealing Preferably the annealing counter should be placed in a very clean
121. ocument will only be maintained online Chapter 5 Laboratory Management Plan Figure 5 1 Flammable Storage Cabinet Figure 5 2 Flammable Safety Can 5 7 3 Compressed Gases Storage Compressed gases are defined as gases that are contained in a receptacle at a pressure not less than 280 kPA at 20 C or as a refrigerated liquid The following guidelines for storing compressed gases must be followed in all laboratories e Compressed gas cylinders cylinders must be stored in a secure well ventilated location and in an upright position at all times e All cylinders should be handled as if full and should never be completely emptied e Cylinders that are not in use meaning that the cap is on must be secured and have the safety cap Multiple cylinders may be secured together gang chained only if they are capped not in use Only capped cylinders can be secured with a single restraining device gang chained as shown in Figure 5 3 e Cylinders that are in use meaning there is a regulator attached must be individually secured by a chain or strap as shown in Figure 5 4 Cylinder valves and regulators should be protected from impact or damage 37 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan Figure 5 3 Not In Use Cylinders Figure 5 4 In Use Cylinders 5 7 4 Reactive Materials Storage Reactive materials include explosives pyrophorics self heating and se
122. of all components of the CHP The Chemical Hygiene Officer must 6 The official version of this document will only be maintained online Chapter 1 Introduction e Inform Laboratory Supervisors of all health and safety requirements and assist with the selection of appropriate safety controls engineering controls administrative controls and PPE e Ensure that Laboratory Supervisors have the necessary resources to maintain compliance with the CHP and that all lab staff receive appropriate training e Actas the liaison between the Laboratory Supervisors and the Chemical Laboratory Safety Committee e Conduct periodic lab inspections and immediately take steps to abate hazards that may pose a risk to life or safety upon discovery of such hazards e Ensure that SOPs and hazard assessments are being prepared e Maintain employee exposure monitoring records when applicable e Help to develop and implement appropriate environmental health and safety policies and procedures e Review and evaluate the effectiveness of the CHP program at least annually and update it as appropriate and e Actively enforce all applicable safety procedures and ensure the contents of the CHP are followed take appropriate actions when safety procedures are not followed 1 5 Radiological amp Environmental Management Department The Radiological and Environmental Management Department REM serves as the environmental health and safety department for Purdue Unive
123. ogen However if waste disposal questions arise please contact the REM Hazardous Materials Management Section at 49 40121 Section 12 Safety Data Sheet SDS A current copy of the SDS for liquid nitrogen must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Section 13 Protocol Procedure Additional lab protocol may be added here Claridge lab uses liquid Nitrogen as a gas source for the vibration isolation tables glove box fill spin coater purge IR spectrometer purge and sample blow off In these uses is it never encountered as a liquid See the compressed gas SOP for more information Additionally 1L liquid is used for IR detector cool down All personnel must have specific training for IT spectrometer cool down This si documents in the procedures section Cooling down the IR detector below To refill the main gas generator dewar refer to the lab procedure document refilling the 180L dewar elsewhere in the document NOTE Any deviation from this SOP requires approval from PI Section 14 Documentation of Training signature of all users is required e Prior to conducting any work with liquid nitrogen designated personnel must provide trai
124. oor and scales gap Self pressurizing vessels weighing gt 100 Plus skin cover to lb gross wrists throat ankles always position blow off valve away from body Frostbite eye Dry ice very cold frozen solids Safety glasses insulated gloves skin impact cover to eloows knees throat closed shoe w socks 2014 CHP Clardige Lab Chapter 1 C ERTIFICATION OF HAZARD ASSESSMENT Skin eye damage Skin eye damage poisoning inhalation of airborne dust Hot liquid rxn mixture water bath oil bath autoclave still Volume gt 1L Hazardous solids gt 100 g any hazardous solid or gt 1 g chemical requiring designated area list at REM web site or High potential for airborne particles 2014 CHP Clardige Lab Splash goggles insulated gloves skin cover to knees elbows throat closed shoe w socks Skin cover to throat wrists ankles emergency shower available in work area Safety glasses goggles for large quantities chemical resistant gloves skin cover to elbows knees throat closed shoes socks Same except skin cover to wrists ankles and only work in hood 26 1 NOTES a Being within reach of potential hazards within reach varies widely depending on scale and conditions of work and will be judged by affected staff in each room Figure 8 Liquid b Unshielded not behind a drawn hood sash or blast shield c Corrosive pH 12 or pH lt 2 5 d Toxic having any poison
125. or shipment When selecting an inner container to be given to REM for an off campus hazardous materials shipment the following guidelines must be followed e The chemical must be compatible with the container For example corrosive chemicals must not be placed in metal containers hydrofluoric acid in any concentration must not be placed in glass containers e Chemical permeability should be considered when selecting a plastic container especially for organic solvents The container must be able to effectively contain the chemical during transportation under normal conditions e The container must have an appropriate lid that is able to close and seal meaning the container will not leak during transportation under normal conditions Any containers that do not properly seal e g beaker flask test tube will not be shipped off campus by REM 5 14 2 Transporting Chemicals on Campus via Purdue Vehicle Purdue University is a state agency and therefore is exempt from Department of Transportation DOT hazardous materials regulations However the intent of the DOT regulations is still required when transporting chemicals on campus using a motor vehicle This essentially means that all chemical containers must be properly packaged labeled and segregated according to hazard class Do not attempt to move large volumes e g greater than 5 gallons in total volume of chemicals across campus If a large volume of chemicals needs to be moved ac
126. or the specific base bath solution being used must be made available to all personnel working in the laboratory at all times To obtain a copy of the SDS contact the chemical manufacturer or REM at 49 46371 Many manufacturers SDSs can be found online on websites such as Sigma Aldrich http www sigmaaldrich com united states html or Siri MSDS Index http hazard com msds Chapter 23 Section 13 Protocol Procedure Additional lab protocol may be added here Claridge lab Provides apron and face shield dedicated to base bath use and requires their use Do not abandon glassware in the basebath If glass is broken in the base bath cease further work and report this to the PI or lab safety coordinator immediately Tab 2 Lab Specific Protocols Requirements Rules NOTE Any deviation from this SOP requires approval from PI Chapter 24 Section 14 Documentation of Training signature of al users is required e Prior to conducting any work with base baths designated personnel must provide training to his her laboratory personnel specific to the hazards involved in working with this substance work area decontamination and emergency procedures e The Principal Investigator must provide his her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer e The Principal Investigator must ensure that his her laboratory personnel have attended appropriate laboratory safety training or refresher
127. order to prevent injury and potential disease transmission These employees should use appropriate PPE e g puncture resistant gloves tools barrier protection sharps waste containers and engineering controls to protect themselves Refer to the REM webpage for detailed procedures regarding sharps handling and disposal procedures https www purdue edu rem home booklets sharps pdf 45 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 12 Equipment Apparatus and Instrument Safety 5 12 1 Centrifuges The following safety guidelines should be followed when operating centrifuges Before centrifugation e Centrifuges must be properly installed and operated only by trained personnel Centrifuges cannot be placed in the hallway of a building they must remain inside of the laboratory e Train each operator on proper operating procedures review the user manual e Use only rotors compatible with the centrifuge Check the expiration date for ultracentrifuge rotors e Check tubes bottles and rotors for cracks and deformities before each use e Make sure that the rotor tubes and spindle are dry and clean e Examine O rings and replace if worn cracked or missing e Never overfill centrifuge tubes don t exceed full e Always cap tubes before centrifugation e Always balance buckets tubes and rotors properly e Check that the rotor is seated on the drive cor
128. ors or freezers e Chemical containers must be in good condition and appropriate for the chemical that they contain and be free from exterior contamination e Fume hoods should not be used as permanent chemical storage areas unless designated as such Not only does this create potentially unsafe conditions by having extraneous chemicals stored near chemical reactions and processes excess chemical 35 The official version of this document will only be maintained online 3 7 2 Chapter 5 Laboratory Management Plan bottles in the hood may also seriously impair the ventilating capacity of the hood Only chemicals being used in the process or experiment being conducted in the hood are allowed to be stored in the hood and should be removed when the process or experiment is complete Chemicals should not be permanently stored on bench tops Avoid storing any chemical containers on the floor Under no circumstance should chemical containers or anything else be stored in aisle ways corridors or in front of doors Hazardous liquids should not be stored on shelves above eye level unless there is a SOP detailing safe handling procedures Chemicals should be stored at an appropriate temperature and humidity level and never be stored in direct sunlight Periodic cleanouts of expired or unneeded chemicals should be conducted to minimize the volume of hazardous chemicals stored in the laboratory Always follow the chemical manufacturer s storage inst
129. ous or irritating effects to human tissue or human health e Chemical resistant gloves glove thickness length and material must be chosen carefully and will be specific to the chemicals mixtures used and the process conditions f Hood 100 exhaust to outside current approval for all work and functioning properly g Chemicals requiring designated areas full list is at http www purdue edu rem home booklets crdalist pdf 2014 CHP Clardige Lab
130. ous substances which includes carcinogens reproductive toxins and acutely toxic substances e Compressed gases 26 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls Work involving more than 1 liter of flammable liquids flammable solids corrosives oxidizers or organic peroxides at one time High hazard tasks can also include work with equipment that creates particularly hazardous conditions Examples include solvent distillation work with high pressure systems hydrogenation work with cryogenic chemicals such as liquid nitrogen etc REM develops SOP templates that can be used by laboratories These SOPs are not complete as is they are templates that must be customized by each laboratory before they are considered complete Instructions for completion are included in each SOP template Laboratories are encouraged to use this template format to develop their own SOPs Contact REM at 765 49 40121 if assistance is needed with developing lab specific SOPs For the up to date list of SOP templates visit the REM website http www purdue edu rem home files sop htm 4 3 2 Required Laboratory Postings The following forms and labels are required to be posted in most campus laboratories The Emergency Contact Door Posting is required for all laboratories and can be found on the REM webpage https www purdue edu rem home forms doorpost pdf The Certification of Hazar
131. oxide toxicity nitric oxide flammable propane and corrosive hydrogen chloride Chapter 5 7 3 of the CHP details compressed gases storage requirements B 1 4 Pyrophoric Self Heating and Self Reactive Materials Pyrophoric material also called spontaneously combustible is a liquid or solid that even in small quantities and without an external ignition source can ignite after coming in contact with the air A eer Self heating material is a solid or liquid other than a pyrophoric substance which by reaction with air and without energy supply is liable to self heat This endpoint differs from a pyrophoric substance in that it will ignite only when in large amounts kilograms and after long periods of time hours or days 89 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions Self reactive material is a thermally unstable liquid or solid liable to undergo a strongly exothermic thermal decomposition even without participation of oxygen air Chapter 5 7 4 of the CHP details the storage requirements for reactive chemicals B 1 5 Water Reactive Materials A water reactive material is a liquid or solid that reacts violently with water to produce a flammable or toxic gas or other hazardous conditions Alkali metals e g sodium potassium and metal hydrides e g calcium hydride are common water reactive materials found in laboratories Chapter
132. person may use it until it is repaired and tested to ensure it is safe for use e Extension cords must be of the three wire type Extension cords and flexible cords must be designed for hard or extra hard usage The rating or approval must be visible e Portable equipment must be handled in a manner that will not cause damage Flexible electric cords connected to equipment may not be used for raising or lowering the equipment e Extension cords must be protected from damage Sharp corners and projections must be avoided Flexible cords may not be run through windows or doors unless protected from damage and then only on a temporary basis Flexible cords may not be run above ceilings or inside or through walls ceilings or floors and may not be fastened with staples or otherwise hung in such a fashion as to damage the outer jacket or insulation e Extension cords used with grounding type equipment must contain an equipment grounding conductor the cord must accept a three prong or grounded plug Operating equipment with extension cords without a grounding plug is prohibited e Attachment plugs and receptacles may not be connected or altered in any way that would interrupt the continuity of the equipment grounding conductor Additionally these devices may not be altered to allow the grounding pole to be inserted into current connector slots Clipping the grounding prong from an electrical plug is prohibited e Flexible cords may only be plugged
133. pirators should be used under any of the following circumstances e Asa last line of defense i e after engineering and administrative controls have been exhausted e When Permissible Exposure Limit PEL has exceeded or when there is a possibility that PEL will be exceeded e Regulations require the use of a respirator e Anemployer requires the use of a respirator e There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Tab 2 Lab Specific Protocols Requirements Rules Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile gloves are recommended for low volume applications Wearing two pairs of nitrile gloves is recommended NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with the specific flammable or combustible liquids being used Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR http www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggl
134. pment of RADIATION LASER TYPE WAVELENGTH PULSE DURATION MAXIMUM OUTPUT written SOPs and hazard assessments discussed in CLASS 4 LASER PRODUCT detail in Chapter 6 for safe performance of the work e Restricting access to areas where hazardous materials are used e Using safety signs or placards to identify hazardous areas designated areas e Labeling all chemicals e Substitution of toxic materials with less toxic materials when possible e Good housekeeping and good personal hygiene such as routine hand washing and regular decontamination of areas that are possibly chemically contaminated such as bench tops and fume hoods e Prohibiting eating and drinking where chemicals are used or stored 4 3 1 Standard Operating Procedures SOPs are written instructions that detail the steps that will be performed during a given procedure and include information about potential hazards and how these hazards will be mitigated SOPs must be prepared by laboratory personnel who are the most knowledgeable and involved with the experimental process However the Laboratory Supervisor is ultimately responsible for approving SOPs regardless of who prepares them The OSHA Lab Standard required SOPs to be developed for all high hazard tasks that are performed in the lab High hazard tasks include any work with the following types of chemicals e Explosives e Water reactive pyrophoric self heating or self reactive chemicals e Particularly hazard
135. pplicable environmental health and safety rules including the contents of the CHP e Identify hazardous conditions or operations in the laboratory and establish SOPs and hazard assessments to effectively control or reduce hazards e Ensure that all laboratory personnel that work with hazardous chemicals receive appropriate training refer to Chapter 9 for detailed training requirements e Maintain written records of laboratory specific training e g PPE training e Ensure that appropriate PPE e g laboratory coats gloves eye protection etc and engineering control equipment e g chemical fume hood are made available in good working order and being used properly e Conduct periodic lab inspections and immediately take steps to abate hazards that may pose a risk to life or safety upon discovery of such hazards and e Actively enforce all applicable safety procedures and ensure that the CHP is followed by lab staff and all visitors including having a progressive disciplinary process for lab staff members that do not comply with safety rules Laboratory Supervisors must ensure that employees receive CHP training and information before any work with hazardous materials occurs Laboratory Supervisors must also ensure 4 The official version of this document will only be maintained online Chapter 1 Introduction that all employees receive annual CHP refresher training The Laboratory Supervisor can provide the training or delega
136. r processes that involve handling a large volume of flammable materials gt 1 liter e If working with a high hazard chemical ensure that others around you know what you are working with and understand the potential hazards e Limit access to areas where chemicals are used or stored by posting signs and or locking doors when areas are unattended e Purchase the minimum amount of hazardous materials necessary to efficiently operate the laboratory e Ensure that adequate storage facilities e g chemical storage rooms flammable safety cabinets and containers are provided for hazardous materials Ensure that hazardous materials are properly segregated by chemical compatibility e Ensure that ventilation is adequate for the chemicals being used Understand how chemical fume hoods function and be able to determine if the hood is not functioning properly e Use good personal hygiene practices Keep your hands and face clean wash thoroughly with soap and water after handling any chemical e Smoking drinking eating and the application of cosmetics are forbidden in areas where hazardous chemicals are in use Confine long hair and loose clothing e Never smell or taste a hazardous chemical Never use mouth suction to fill a pipette e When using equipment that creates potential hazards e g centrifuge ensure that the equipment is being used following the manufacturer s guidelines and instructions If equipment requires routine maintenance
137. rator e Anemployer requires the use of a respirator e There is potential for harmful exposure due to an atmospheric contaminant in the absence of PEL e As PPE in the event of a chemical spill clean up process Lab personnel intending to use wear a respirator mask must be trained and fit tested by REM This is a regulatory requirement http www purdue edu rem home booklets RPP98 pdf Hand Protection Gloves must be worn Use proper glove removal technique to avoid any skin contact Nitrile gloves layered underneath butyl rubber gauntlet style gloves are recommended Check the resources below for the most suitable glove NOTE Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with the specific base bath solution being used Refer to glove selection chart from the links below http www ansellpro com download Ansell 8thEditionChemicalResistanceGuide pdf OR http www showabestglove com site default aspx OR htto www mapaglove com Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles are required A face shield may also be necessary when there is a potential for splashes Skin and Body Protection Laboratory coats must be worn and be appropriately sized for the individual and buttoned to their full length Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves Personnel should
138. rectly close the lid on the centrifuge and secure it e When using swinging bucket rotors make sure that all buckets are hooked correctly and move freely During centrifugation e Close lids at all times during operation Never open a centrifuge until the rotor has stopped e Do not exceed safe rotor speed e The operator should not leave the centrifuge until full operating speed is attained and the machine appears to be running safely without vibration e Stop the centrifuge immediately if an unusual condition noise or vibration begins and check load balances 46 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan After centrifugation e Allow the centrifuge to come to a complete stop before opening e Wear new pair of outer gloves to remove rotor and samples e Check inside of centrifuge for possible spills and leaks clean centrifuge and rotor thoroughly if necessary e Wash hands after removing gloves 5 12 2 Stirring and Mixing Equipment Stirring and mixing devices commonly found in laboratories include stirring motors magnetic stirrers and shakers These devices are typically used in lab operations that are performed in a chemical fume hood and it is important that they be operated ina way that prevents the generation of electrical sparks Only spark free induction motors should be used in power stirring and mixing devices or any other rotating equip
139. rom the workplace or substitute a less hazardous technique process or material If elimination or substitution are not feasible options administrative controls engineering controls and PPE must be used to provide the necessary protection The laboratory employee s responsibility is to follow administrative controls use engineering controls and wear PPE correctly and effectively 4 1 Routes of Exposure There are four primary routes of exposure in which hazardous substances can enter the body inhalation absorption ingestion and injection Of these the most likely routes of exposure in the laboratory are by inhalation and or skin absorption Many hazardous chemicals may affect people through more than one of these exposure modes so it is critical that protective measures are in place for each of these exposure routes 4 2 Engineering Controls and Safety Equipment Exposure to hazardous materials must be controlled to the greatest extent feasible by use of engineering controls Engineering controls to reduce or eliminate exposures to hazardous chemicals include e Substitution with less hazardous equipment chemicals or processes e g safety cans for glass bottles e Isolation of the operator or the process e g use of a glove box when handling air or water sensitive chemicals and e Use of forced ventilation systems e g chemical fume hood biological safety cabinet 4 2 1 Chemical Fume Hoods A chemical fume hood is a
140. ross campus such as an entire lab move contact REM 765 49 40121 53 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan for further assistance The following procedures must be followed in order to properly and legally transport chemicals across campus e Only Purdue owned vehicles are permitted to be used to transport chemicals For liability and insurance purposes no personal vehicles should ever be used to transport hazardous chemicals e Ensure that each container has an appropriate tight fitting lid The lid should have the ability to contain the contents of the container even if it becomes inverted during transport Examples of inappropriate lids include cracked caps loosely fitting rubber stoppers or Parafilm e Chemicals should be segregated according to the primary hazard class For example do not place an oxidizer such as ammonium nitrate in the same container as an organic solvent such as acetone e All containers should be packaged upright e Chemical containers should be placed in some type of outer packing such as a box bin or bucket Containers should remain securely packaged during loading transport and unloading Glass to glass contact should be avoided Bubble wrap newspaper and vermiculite are good examples of packaging material that will prevent glass to glass contact e The outer containers should remain tightly secured during transport Measures sho
141. rsity REM s primary role is to manage regulatory compliance with all federal state and Purdue regulations involving environmental health and safety issues REM facilitates a number of programs that apply to laboratory safety a few of which include biological safety laser safety personal protective equipment program radiation safety development of standard operating procedures as well as the CHP REM also performs numerous safety inspections of facilities throughout the year to monitor compliance with regulatory requirements REM provides a variety of services such as training chemical biological and radioactive waste pickups and safety consultation More detailed information regarding all of REM s resources and services can be found on the REM website http www purdue edu rem 1 6 Integrated Safety Plan It is the policy of Purdue University to integrate environmental health and safety into all operations The Integrated Safety Plan ISP is Purdue University s safety program which is facilitated by REM and was developed to provide a framework for laboratories to comply with environmental health and safety EHS regulations The ISP assists in communication of EHS 7 The official version of this document will only be maintained online Chapter 1 Introduction issues across the organization and calls for departmental level safety committees and individual self audits The ISP provides indemnification from regulatory fines for
142. ructions if provided Flammable Liquids Storage Flammable liquids include any liquid with a flash point no greater than 93 C 200 F The following guidelines for storing flammable liquids must be followed in all laboratories Flammable and combustible liquids should be stored in flammable storage cabinets as shown in Figure 5 1 whenever possible No more than 10 gallons of flammable liquid is permitted to be stored outside of a flammable storage cabinet unless it is stored in a flammable safety can equipped with a spring loaded lid and an internal screen as shown in Figure 5 2 Domestic refrigerators or freezers must never be used to store flammable liquids Flammable liquids can only be stored in refrigerators or freezers that are designed for flammable materials most refrigerators are not intended for flammable storage Flammable liquids must be stored in well ventilated areas free from ignition sources Some organic solvents e g diethyl ether have a shelf life and can form organic peroxides over time while in storage These peroxide formers must be dated when received from the chemical manufacturer and disposed of once expired If any time sensitive chemicals are found to be past the manufacturer s expiration date they must be submitted to REM for hazardous waste disposal immediately See Appendix C for a list of commonly found organic solvents that potentially form organic peroxides 36 The official version of this d
143. s should be stored in a gas cylinder cabinet as shown in Figure 3 e Do not over purchase only purchase what can be safely stored in the laboratory e Avoid contact with skin eyes and inhalation e Keep away from sources of ignition if the gas is flammable Tab 2 Lab Specific Protocols Requirements Rules Figure 1 Not In Use Cylinders Figure 2 In Use Cylinders Figure 3 Gas Cylinder Cabinet e Follow laboratory supervisor s instructions for PPE which may differ depending on the type and or quantity of compressed gas being used e Use inthe smallest practical quantities for the experiment being performed e Work must be conducted in a chemical fume hood if air concentrations above 10 of the LEL could be created if the chemical is irritating to the eyes or respiratory system and or is toxic by inhalation e Gas cylinder connections and fittings must be inspected frequently for deterioration and must never be used without a regulator e Never use a leaking corroded or damaged cylinder and never refill compressed gas cylinders e When stopping a leak between cylinder and regulator always close the valve before tightening the union nut e The regulator should be replaced with a safety cap when the cylinder is not in use e The safety cap must be in place when a gas cylinder is moved e For large gas cylinders gt 27 inches an approved gas cylinder cart should be used The Figure 4 Cylinder Cart cylinder must be
144. s the basic mechanical functionality of each station Any deficiencies are repaired either by REM staff or by Purdue Physical 24 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls Facilities maintenance staff If the safety shower or eye wash unit becomes inoperable notify your building deputy immediately 4 2 6 Fire Extinguishers All fire extinguishers should be mounted on a wall in an area free of clutter Each fire extinguisher on campus is inspected on an annual basis by the Purdue Fire Department All laboratory personnel should be familiar with the location use and classification of the extinguishers in their laboratory Ensure that the fire extinguisher being used is appropriate for the type of material on fire before attempting to extinguish any fire Table 4 1 illustrates the fire classification system which should be used to determine the most suitable fire extinguisher for a particular area Laboratory personnel are not required to extinguish fires that occur in their work areas and should not attempt to do so unless e tisasmall contained fire that can be quickly and safely extinguished e g small trash can sized fire e Appropriate training has been received and the individual feels the fire can be safely extinguished and e tis necessary to extinguish a fire in order to exit an area e g fire is blocking an exit If a fire occurs in the laboratory and is ex
145. se of Fire Use water spray alcohol resistant foam dry chemical or carbon dioxide First Aid Move out of dangerous area Consult a physician If inhaled move person to fresh air If not breathing give artificial respiration In case of skin contact wash with soap and plenty of water In case of eye contact rinse thoroughly with plenty of water for at least 15 minutes If swallowed do not induce vomiting Never give anything by mouth to an unconscious person Rinse mouth with water consult a physician Figure 2 3 GHS Label Format As mentioned earlier one of the objectives of GHS was to create a quantitative hazard classification system numerical hazard classification is based on a 1 5 scale 1 being the most hazardous and 5 being the least hazardous based on physical characteristics such as flash point boiling point lethal dose of 50 of a population reactivity etc Table 2 1 illustrates how the numerical hazard classification works for flammable liquids More detailed information on GHS can be found on the OSHA website https www osha gov dsg hazcom ghs html 12 The official version of this document will only be maintained online Chapter 2 Chemical Classification Systems Table 2 1 GHS Hazard Classification System for Flammable Liquids Category Criteria Pictogram Signal Word Hazard Statement Flash point lt 23 C Extremely flammable ae l Danger a Boiling point lt 35 C liquid and vapor Flash
146. se rapid suffocation due to displacement of oxygen With asphyxiation unconsciousness may happen without warning If person becomes dizzy move them to a well ventilated area and seek immediate medical attention dial 911 In case of skin contact Skin contact with liquid nitrogen may cause severe cold burns and frostbite Flesh freezes very rapidly and may be torn when attempting to be withdrawn from object If frostbite or freezing occurs the following steps should be taken 1 Flush the area thoroughly with tepid water Do not apply heat or rub the affected area 2 Protect the area with bulky dry and sterile dressings 3 Seek immediate medical attention dial 911 In case of eye contact Eye exposure to liquid nitrogen can cause permanent and irreversible damage Delicate eye tissue can be damaged by exposure to the cold gas alone If liquid nitrogen is splashed into the eyes the following steps should be taken flush the eyes with water for 15 minutes and seek immediate medical attention dial 911 Section 10 Medical Emergency Life Threatening Emergency After Hours Weekends And Holidays Dial 911 Non Life Threatening Emergency Immediately report injury to supervisor and complete the First Report of Injury http www purdue edu rem injury froi htm Section 11 Waste Disposal Procedures Tab 2 Lab Specific Protocols Requirements Rules There is typically no waste generation involved with the use of liquid nitr
147. ssociated with each body part e Determine PPE requirements for each hazard e List other control measures required such as engineering and administrative controls See the REM webpage for the task evaluation hazard assessment template http www purdue edu rem home files forms htm ppe 6 3 2 Location Evaluation Hazard Assessment Location evaluation hazard assessments should be conducted for specific areas laboratories These types of hazard assessments should be written in a comprehensive manner that includes the majority of hazards present in a specific location e g flammable and corrosive liquids This type of hazard assessment is the most commonly used in laboratories and should be posted in a location within the lab where it is easily accessed by personnel e g posted near the front door of the lab If employees perform specific tasks not covered by the laboratory hazard assessment then it will be necessary to perform another type of hazard assessment such as the task evaluation assessment that does address the specific hazards of that task The following describes the steps that should be taken to perform a task evaluation hazard assessment e Identify the hazards e List each task where hazard is present e Determine PPE requirements for each task e List other control measures required engineering and administrative controls 62 The official version of this document will only be maintained online Chapter 6 Laboratory
148. ssseesssrreserreseerssee 7 LO integrated Satety Plaioiussirs naea a a ees 7 1 7 Chemical and Laboratory Safety CoMmMittee seseusseeneseesssresssrrssrerssrerssereserreseersseersseee 8 Chapter 2 Chemical Classification SystemS sesessssesesesecssosoececesssseoesesssssosoecessessseosoessssese 9 2 1 Globally Harmonized System for Classifying Chemicals cccccssecccssececeeseeeeeseceseeeeeseees 9 ZV SavetyiDatar SMCCUS aa a sere team eseamaetanan ess 9 22 Chemicaltabelne sureni i a nna T N IAA bane eet nasa T A 10 2 2 National Fire Protection Association Rating System ssesesseessseesssressrrresrerssrersrresseeresee 13 2 3 Department of Transportation Hazard Classes ccccccssecccssecccessceceeseeeceeceeenecesseceseees 14 Chapter 3 Classes of Hazardous Chemicals ccscsccscscsccsccccsccccsccccscsscccsccccsceccscecsscesceees 16 SE PAV EHZ A aa E E E E E 16 3 2 Health Hazards sacsunrucrenaninaii aT A E EAE RAN 17 3 SBIOIOLICAI H AZAA S ranner E E a E O 18 3 4 RAGIOACTIVE Material HAZAOS si siecseu deceit niivcecnwtaees i E OAN 18 Fe mrs Si Aa 60 cee T Cr 18 Chapter 4 Laboratory Safety Controls sessssesesesesssosoecesesssosoeoecesssosoesesssseoeossesssosoeoessesese 19 4 1 Engineering Controls and Safety EQuipMent sssessssessseessseesssrrsserreseerssressseeserresereeseeees 19 ALi Chemical Fume OOO S asnasa ma Yas wan sah a aa 19 Ae OVE DOE a PE re PRR ern Pa PDR ee A er
149. st wear protective clothing to protect the body from recognized hazards All unprotected skin surfaces that are at risk of injury should be covered Full length pants or full length skirt must be worn at all times by all individuals that are occupying the laboratory area shorts are not permitted Lab coats coveralls aprons or protective suits are required to be worn while working on or adjacent to all procedures using hazardous chemicals e g chemical bottle is open and the chemical is being poured transferred pipetted etc Laboratory coats must be appropriately sized for the individual and be fastened snap buttons are recommended to their full length Laboratory coat sleeves must be of a sufficient length to 66 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy prevent skin exposure while wearing gloves Flame resistant laboratory coats must be worn when working with pyrophoric materials or flammable liquids greater than 1 liter in volume It is recommended that 100 cotton or other non synthetic material clothing be worn during these procedures to minimize injury in the case of a fire emergency Laboratory coats should not be worn outside of a laboratory unless the individual is traveling directly to an adjacent laboratory work area Laboratory coats should not be worn in common areas such as break rooms offices or restrooms Each department is responsible for providing
150. strapped to the cart and the protective cap must be in place before moving the cylidner A cylinder should never be moved or transported without the protective cap The proper way to move a large gas cylinder is illustrated in Figure 4 e A few compressed gas cylinders have a shelf life and can become more hazardous as time goes on It is extremely important that these chemicals are identified and managed properly If any time sensitive gases are found to be past the manufacturer s expiration date they must be submitted to REM for hazardous waste disposal immediately The following is a list of time sensitive compressed gases o Hydrogen fluoride anhydrous o Hydrogen bromide anhydrous o Hydrogen sulfide anhydrous o Hydrogen cyanide anhydrous o Hydrogen chloride anhydrous Section 9 Spill and Accident Procedures Chemical Spill Dial 911 Tab 2 Lab Specific Protocols Requirements Rules Immediately evacuate area and ensure others are aware of the spill If there is an imminent threat of a fire pull the nearest fire alarm station to evacuate the building and dial 911 Chemical Spill on Body or Clothes Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes Seek medical attention dial 911 Chemical Splash into Eyes Immediately rinse eyes and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open Seek medical attention dial 911
151. t of an emergency chemical spill e Cease all activities and immediately evacuate the affected area make sure that all personnel in the area are aware of the spill and also evacuate e If chemical exposure has occurred to the skin or eyes the affected personnel should be taken to the nearest safety shower and eyewash station 80 The official version of this document will only be maintained online Chapter 8 Chemical Spills e Dial 911 which will initiate both the Purdue Police and Purdue Fire Department response if the situation is or could become an emergency e g chemical exposure has occurred a fire or explosion has occurred e The fire alarm should be pulled which will initiate building evacuation if any of the following occurs o A fire and or explosion has occurred or there is a threat of fire and or explosion o The large spill which is either highly toxic or presents an immediate fire or environmental hazard is in a public area such as a hallway o Toxic vapors are leaving the area where the spill has occurred such as seeping from the laboratory into the hallway or neighboring rooms o You are unsure of the hazards and feel that the spill could be harmful to building occupants o Ensure that no one else is allowed to enter the area until the spill has been properly cleaned up by the Purdue Fire Department 8 3 Chemical Spill Kits Each laboratory should have a spill response kit available for use Lab spil
152. t produce toxic effects on the nervous system such as mercury acrylamide and carbon disulfide e Substances that act on the hematopoietic system e g carbon monoxide and cyanides which decrease hemoglobin function and deprive the body tissues of oxygen e Substances that damage lung tissue such as asbestos and silica B 2 5 Particularly Hazardous Substances Substances that pose such significant threats to human health are classified as particularly hazardous substances PHSs The OSHA Laboratory Standard requires that special provisions be established to prevent the harmful exposure of researchers to PHSs including the establishment of designated areas for their use Particularly hazardous substances are divided into three primary types 1 Carcinogens 92 The official version of this document will only be maintained online Appendix B OSHA Hazard Class Definitions 2 Reproductive Toxins 3 Substances with a High Acute Toxicity B 2 5 1 Carcinogens Carcinogens are chemical or physical agents that cause cancer Generally they are chronically toxic substances that is they cause damage after repeated or long duration exposure and their effects may only become evident after a long latency period Chronic toxins are particularly insidious because they may have no immediately apparent harmful effects These materials are separated into two classes 1 Select Carcinogens Select carcinogens are materials which have met cer
153. t the process e Are my storage facilities appropriate for the type of materials will be using e Can l properly segregate incompatible chemicals e What possible accidents can occur and what steps can take to minimize the likelihood and impact of an accident What is the worst incident that could result from my work 29 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 1 2 General Laboratory Safety Rules It is extremely important that all laboratory safety rules are known and followed by lab personnel Not only is it important that the rules are understood and followed it is also important that the Laboratory Supervisor enforce all lab safety rules A culture of safety must be adopted by all employees before a lab safety program can be successful The following general laboratory safety rules should be followed at all times e Prior to beginning work in the lab be prepared for hazardous materials emergencies and know what actions to take in the event of an emergency Plan for the worst case scenario Be sure that necessary supplies and equipment are available for handling small spills of hazardous chemicals Know the location of safety equipment such as the nearest safety shower and eyewash station fire extinguisher spill kit and fire alarm pull station e Do not work alone in the laboratory if you are working with high hazard materials e g acutely toxics reactives o
154. tain criteria established by the National Toxicology Program or the International Agency for Research on Cancer regarding the risk of cancer via certain exposure routes It is important to recognize that some substances involved in research laboratories are new compounds and have not been subjected to testing for carcinogenicity 2 Regulated Carcinogens Regulated carcinogens are more hazardous and have extensive additional requirements associated with them The use of these agents may require personal exposure sampling based on usage When working with Regulated Carcinogens it is particularly important to review and effectively apply engineering and administrative safety controls as the regulatory requirements for laboratories that may exceed long term 8 hour or short term 15 minutes threshold values for these chemicals are very extensive B 2 5 2 Reproductive Toxins Reproductive toxins include any chemical that may affect the reproductive capabilities including chromosomal damage mutations and effects on fetuses teratogens Reproductive toxins can affect the reproductive health of both men and women if proper procedures and controls are not used For women exposure to reproductive toxins during pregnancy can cause adverse effects on the fetus these effects include embryolethality death of the fertilized egg embryo or fetus malformations teratogenic effects and postnatal functional defects For men exposure can lead to sterility
155. te this task to a qualified individual e g Laboratory Safety Officer senior lab employee The CHP training must be documented See Appendix A for CHP Awareness Certification Form which can be used to document CHP training Failure to follow the requirements of the CHP could possibly result in injuries fines from regulatory agencies such as OSHA and or disciplinary action 1 4 2 Laboratory Employee Responsibilities All employees e g lab technicians graduate students undergraduate students post doctoral researchers and visiting scientists in laboratories that use handle or store hazardous chemicals must 1 Review and follow the requirements of the CHP Follow all verbal and written laboratory safety rules regulations and SOPs required for the tasks assigned 3 Develop and practice good personal chemical hygiene habits such keeping work areas clean and uncluttered 4 Plan review and understand the hazards of materials and processes in the laboratory prior to conducting work 5 Utilize appropriate measures to control hazards including consistent and proper use of engineering controls administrative controls and PPE 6 Understand the capabilities and limitations of PPE 7 Immediately report all accidents near misses and unsafe conditions to the laboratory Supervisor 8 Complete all required REM and or other mandatory safety training and provide written documentation to the laboratory supervisor 9 Participate
156. ted in Figure 2 should be worn when directly handling a sealed cryogenic tube Self Pressurizing Tanks Self pressurizing tanks Figure 7 are generally a 140 260 L double wall stainless steel tank used to fill other liquid nitrogen containers such as Dewars These tanks are equipped with pressure relief valves and a backup rupture disk A loud hissing sound is commonly heard when the pressure relief valve opens Exposure to liquid nitrogen can occur when connecting and disconnecting equipment during the filling process from a leaking valve or from condensate ice buildup on valves and hoses Because of these risks the PPE illustrated in Figure 3 should always be worn when working with a self pressurized tank Section 8 General Safe Handling Practices and Storage Requirements e Only trained personnel should work with liquid nitrogen e Use only in well ventilated and low traffic areas e Caution signs should be posted in the area warning others that liquid nitrogen is being stored and used e Always wear the appropriate PPE e Liquid nitrogen should only be stored in approved containers e All liquid nitrogen containers must be labeled Large containers e g Dewars Self Pressurizing Tanks must be labeled with the REM provided label illustrated in Figure 8 Smaller containers such as cryogenic tubes should be labeled Liquid Nitrogen Cryogenic Hazard or with similar words that convey the hazards e Avoid breathing liquid ni
157. the surface is hot enough that water that condenses on the surface evaporates almost immediately This may take 1 2 minutes Decrease the flame length to 1 by slowly turning the pin valve clockwise Gently set the quartz tip down across the top of the cork ring to prevent the flame from scorching anything around it Set the gas lighter across the tube to prevent it from sliding around on the bench Place the sample on the glass slide in the center of the metal plate Leave 1 2 mm of mica hanging over the edge of the slide for easy sample pickup later If too much of the mica is exposed it may overheat and curl as the sample is annealed Anneal the sample Bring the flame tip to the gold film at about a 30 degree angle Sweep back and forth at approx 1 Hz freq for 15 20 seconds DO NOT OVERHEAT THE FILM The sample should glow minimally if at all Turn off the flame Turn the pin valve clockwise until closed finger tight The flame should stop Turn off the gas at the main valve Decrease the pressure at the regulator by turning counterclockwise until it turns easily Purge the gas from the regulator Open the pin valve Hold the quartz tip at the junction with the tubing to prevent it from flying off when gas pressure increases Slowly increase the regulator pressure turn clockwise until the gas audibly flows from the line Decrease regulator pressure turn counterclockwise and close the pin valve S
158. thyl acetate xylene are more hazardous at elevated temperatures due to more rapid vaporization The following definitions are important to understand when evaluating the hazards of flammable liquids e Flammable liquid is a liquid having a flash point no greater than 93 C 200 F e Flash point is the minimum temperature at which the ae application of an ignition source causes the vapors of a 0 AIR liquid to ignite under specified test conditions e Boiling point is the temperature at which the vapor pressure of a liquid equals the atmospheric pressure and the liquid changes into a vapor e Auto ignition temperature is the minimum temperature at which self sustained combustion will occur in the absence of an ignition source FLAMMBLE e Lower explosive limit LEL is the lowest concentration RANGE percentage of a gas or a vapor in air capable of producing a flash of fire in presence of an ignition source TOO LEAN arc flame heat a E 0 GAS e Upper explosive limit UEL is the highest concentration 100 AIR percentage of a gas or a vapor in air capable of producing a flash of fire in presence of an ignition source arc flame heat Some organic solvents e g diethyl ether have the potential to form potentially shock sensitive organic peroxides See Appendix 3 for additional information regarding peroxide forming chemicals Chapter 5 7 2 of the CHP details flammable liquids storage requirements B 1 2 Flammable
159. tinguished by lab personnel the Purdue University Fire Department must still be contacted immediately by dialing 911 Table 4 1 Fire Classifications System Classification Fire Type ClassA Ordinary fire wood and paper Flammable liquids and gases ClassC__ Electric fire ClassD Combustible metal fire Kitchen fire 4 2 7 Fire Doors Many laboratories may contain fire doors as part of the building design These doors are an important element of the fire containment system and should remain closed unless they are on a magnetic self closure or other automated self closing system Never disable an automatic door closure device e g placing a block under the door If you are unsure of whether a door is fire rated or not contact REM at 765 49 46371 and a staff member will come to the area to evaluate the specific door in question 25 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls 4 3 Administrative Controls Administrative controls are procedural measures which can be taken to reduce or eliminate hazards associated with the use of hazardous materials Administrative controls include the following e Ensuring that employees are provided adequate documented training for safe work with hazardous DANGER materials e Careful planning of experiments and procedures with n AET ce ATION auni 7 P E TO DIRECT OR SCATTERED safety in mind Planning includes the develo
160. tion and guidelines for acceptable sink disposal is provided in the Purdue University Hazardous Waste Disposal Guidelines Chapter 5 6 Appendix E and Appendix F http www purdue edu rem home booklets hwdg pdf Please contact REM at 765 49 40121 for further information regarding non hazardous chemical waste disposal 7 8 Sharps Waste Sharps are items capable of puncturing cutting or abrading the skin such as glass or plastic pipettes broken glass test tubes petri dishes razor blades needles and syringes with needles Sharps waste contaminated with hazardous chemicals must be placed into puncture resistant containers e g sharps container plastic or metal container with lid and properly labeled as detailed in Chapter 4 of the Purdue University Hazardous Waste Guidelines All chemically contaminated waste should be inventoried on a Hazardous Materials Pickup Request Form and sent to REM for proper disposal Clean uncontaminated broken glassware and plastic sharps should be placed in a corrugated cardboard box or other strong disposable container Do not exceed 20 pounds When ready for disposal the box should be taped shut and prominently labeled as Sharp Objects Glass Discard or similar wording The Safe for Disposal label Figure 7 5 should also be affixed to the outside of the container Contact your Physical Facilities Building Services department for specific non hazardous waste disposal instructions More detail
161. tions Employees must notify their Laboratory Supervisor of all injuries and illnesses regardless of how the magnitude The laboratory supervisor must ensure that a First Report of Injury form is completed Employees should report to a Purdue approved occupational medical provider http www purdue edu hr Benefits wcimmediatecare html treatmentFacilities if medical attention is required Note The Purdue University Student Hospital is not an approved occupational medical provider If the injury is serious and presents an emergency situation dial 911 and emergency responders Purdue Fire Department if located on the West Lafayette Campus will respond and transport the patient to a local hospital emergency room For more information regarding the First Report of Injury reporting process visit the REM webpage http www purdue edu rem injury froi htm Departments must provide all employees who work with hazardous chemicals an opportunity to receive medical attention including any follow up examinations which the examining physician determines to be necessary under the following circumstances e Whenever an employee develops signs or symptoms associated with a hazardous chemical to which the employee may have been exposed in the laboratory e Where exposure monitoring reveals an exposure level routinely above the action level or in the absence of an action level the permissible exposure limit for an OSHA 68 The official version of this docu
162. torage in beakers or flasks should be temporary If temporarily storing samples in beakers or flasks a cork Parafilm or some other closure device must be used e All samples must be labeled with the chemical name date the sample was developed received and the name of generator Chemical structure or a labeling system that is only known to lab personnel is not acceptable as the only means of labeling samples Acronyms can be used as a labeling system as long as an up to date legend is posted in the lab e Samples should be disposed of within 6 months unless actively being used for analysis Stockpiling unusable samples in not an acceptable practice All samples that are no longer necessary must be properly disposed of in a timely manner using REM s hazardous waste program e Samples must be stored according to the primary hazard class this should be done to the best of your ability considering the properties that are known or assumed such as toxicity e lf the hazard s of a sample are unknown the Laboratory Supervisor must attempt to determine whether it is hazardous or not Assume all samples are toxic unless otherwise 51 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan demonstrated This can be accomplished by literature review or reviewing the hazards of other similar compounds At a minimum the Laboratory Supervisor should be able to determine if a chemical is fl
163. trained to work safely with these substances This includes custodial and maintenance personnel support staff who work to maintain laboratories All personnel including principal investigators laboratory supervisors laboratory technicians student workers and support staff have a responsibility to maintain a safe work environment All personnel working with chemicals are responsible for staying informed on the chemicals in their work areas safe work practices and SOPs and proper personal protective equipment PPE required for the safe performance of their laboratory work 1 4 1 Laboratory Supervisor Responsibilities The Laboratory Supervisor is the individual that is ultimately responsible for the overall laboratory operation including the lab safety program and ensuring that the requirements of the CHP are followed by all staff members that work in the lab For most research laboratories the Principal Investigator PI is the Laboratory Supervisor In cases where the PI has hired an individual such as a lab manager or postdoctoral scholar to manage the daily operations of the lab the PI is still ultimately responsible for the overall operation of the lab and is considered to be the Laboratory Supervisor The Laboratory Supervisor may delegate some safety duties to a qualified individual but ultimately remains responsible for the safety of all personnel working in the laboratory Specifically the Laboratory Supervisor must e Understand a
164. trogen vapors e Carry containers away from body and face e Never drop aliquid nitrogen container Damage to a container may result in over pressurization or container failure e Dewars more than 100 pounds require two people to move safely e Always use a specially designed cylinder cart to transport liquid nitrogen containers that are too heavy to be hand carried e Use the freight elevator whenever possible e Do not leave open containers unattended e Liquid nitrogen containers should be stored in cool dry and well ventilated areas e Do not store in a cold room or other controlled environment without air supply e Liquid nitrogen containers should be stored out of direct sunlight Section 9 Spill and Accident Procedures In the event of a large liquid nitrogen spill or release immediately evacuate the area and ensure others are aware of the spill Remember that frostbite and asphyxiation are the primary hazards so ensure people are protected Tab 2 Lab Specific Protocols Requirements Rules from these hazards If there is an imminent threat pull the nearest fire alarm station to evacuate the building and dial 911 If the spill is minor and does not pose a threat to personnel contact REM at 49 40121 during normal business hours 7 AM 4 PM for spill cleanup assistance dial 911 if spill occurs after hours and assistance is needed Section 7 First Aid Procedures If inhaled Over exposure of liquid nitrogen may cau
165. ty Committee Indiana Department of Environmental Management Integrated Safety Plan Liquid Chromatography Lethal Concentration 50 Lethal Dose 50 Lower Explosive Limit Laser Safety Committee Material Safety Data Sheet National Fire Protection Association Occupational Safety and Health Administration Polychlorinated Biphenyl Particularly Hazardous Substance Principal Investigator Personal Protective Equipment Resource Conservation and Recovery Act Recombinant Deoxyribonucleic Acid Radiological and Environmental Management Radiation Safety Committee Satellite Accumulation Area Safety Data Sheet Standard Operating Procedure Upper Explosive limit 1 The official version of this document will only be maintained online Chapter 1 Introduction Chapter 1 Introduction Laboratory safety is an integral part of laboratory research and is essential to ensure that Purdue University s compliance with all applicable environmental health and safety laws regulations and requirements are met The risks associated with laboratory research workplace injuries environmental incidents and property losses or damage are greatly reduced or eliminated when proper precautions and practices are observed in the laboratory To better manage and mitigate these risks Purdue University has developed the Chemical Hygiene Plan CHP which is intended to be the cornerstone of your laboratory safety program and is designed to aid faculty staff
166. type of local ventilation installation that is designed to limit exposure to hazardous or toxic fumes vapors or dusts To determine if a chemical is required to be used inside of a chemical fume hood first check the SDS for that chemical Statements found in Section 2 on a SDS such as do not breathe dust fumes or vapors or 19 The official version of this document will only be maintained online Chapter 4 Laboratory Safety Controls toxic by inhalation indicate the need for ventilation As a best practice always use a chemical fume hood for all work involving the handling of open chemicals e g preparing solutions whenever possible If a chemical fume hood is required or recommended to be used the following guidelines must be followed at all times Chemical fume hoods must be marked to indicate the proper sash position for optimum hood performance as illustrated in Figure 4 1 The chemical fume hood sash should be positioned at this height whenever working with hazardous chemicals that could generate toxic aerosols gases or vapors In general the sash height should be set at a level where the operator is shielded to some degree from any splashes explosions or other violent reactions which could occur and where optimum air flow dynamics are achieved Most chemical fume hoods are not intended to be used with the sash fully open The sash should only be fully opened to add or remove equipment from the chemical fume hood
167. uch a temperature and pressure and at such a speed that can cause damage to the surroundings Pyrotechnic substances are included even when they do not evolve gases A pyrotechnic substance or mixture is designed to produce an effect by heat light sound gas or smoke ora combination of these as the result of non detonative self sustaining exothermic chemical reactions An explosive compound that is sometimes found in a laboratory setting is picric acid 2 4 6 trinitrophenol If a laboratory plans to work with explosive compounds contact REM for further instructions before any work occurs B 2 Health Hazards A chemical is a health hazard if there is statistically significant evidence based on at least one study conducted in accordance with established scientific principles that acute or chronic health effects may occur in exposed employees Each health hazard is defined and briefly discussed below B 2 1 Irritants lrritants are defined as chemicals that cause reversible inflammatory effects on living tissue by chemical action at the site of contact A wide variety of organic and inorganic compounds including many chemicals that are in a powder or crystalline form are irritants Symptoms of exposure can include reddening or discomfort of the skin and irritation to respiratory systems B 2 2 Sensitizers A sensitizer allergen is a substance that causes exposed individuals to develop an allergic reaction in normal tissue aft
168. uld be taken to avoid movement of the outer containers For example the containers should be secured using a strap or an empty box can be used to fill the gap between the last box and the sidewall of the vehicle e The outer container must be labeled in a manner that identifies the contents e g corrosives flammables e Transport with two or more people if possible e Be prepared for unseen accidents At least one person should be knowledgeable of the materials being transported An inventory with an estimated volume or weight per hazard should be recorded and available during transport e g 5 gallons of flammable liquid and 10 pounds of toxic solids e Prepare a spill kit prior to transport Material such as appropriate PPE absorbent material and an empty bucket is sufficient for most small spills e Carry acell phone and know who to call in the event of an emergency The Purdue Fire Department will respond to on campus emergencies Dial 911 from a Purdue phone or 765 49 48221 from a cell phone to contact Purdue Police dispatch 54 The official version of this document will only be maintained online Chapter 5 Laboratory Management Plan 5 14 3 Transporting Chemicals on Campus via Foot Transporting small volumes of chemicals across campus via foot e g from two neighboring campus buildings is acceptable as long as it is done properly Do not attempt to move large volumes e g greater than 5 gallons in total volume of chemica
169. ument will only be maintained online Chapter 5 Laboratory Management Plan mens ORGANOMETALLICS PYROPHORICS Figure 5 5 Reactive Chemicals Storage Many reactive materials are water and or air reactive and can spontaneously ignite on contact with air and or water Therefore reactives must be handled under an inert atmosphere and in such a way that rigorously excludes air and moisture If reactive materials are received in a specially designed shipping storage or dispensing container such as the Aldrich Sure Seal packaging system ensure that the integrity of that container is maintained Ensure that sufficient protective solvent oil kerosene or inert gas remains in the container while reactive materials are stored 5 7 5 Acutely Toxic Materials Storage Acutely toxic materials are defined as substances that may be fatal or cause damage to target organs as the result of a single exposure or exposures of short duration The following guidelines for storing acutely toxic materials must be followed in all laboratories Suitable storage locations for acutely toxic materials include desiccators glove boxes flammable storage cabinets that do not contain incompatible chemicals primarily strong acids or non domestic refrigerators or freezers These locations should be clearly posted Acutely toxic materials should be stored in secondary containment at all times as a best management practice If possible store al
170. uoroethylene Divinylacetylene Vinylidine chloride Group B chemicals form peroxides that are hazardous only on concentration by distillation or evaporation Test these before distillation and discard after 12 months 96 The official version of this document will only be maintained online Appendix C Peroxide Forming Chemicals Group C chemicals consist of monomers which form peroxides that can initiate explosive polymerization Inhibited monomers should be tested before use and discarded after 12 months Uninhibited monomers should be discarded 24 hours after opening Table C 3 Group C Chemicals Acrylonitrile Tetrafluoroethylene Chlorotrifluoroethylene Methyl methacrylate General Guidelines e Solvents containing inhibitors should be used whenever possible e All peroxide forming solvents should be tested prior to distillation e Peroxide forming solvents should be purchased in limited quantities e Peroxide forming solvents should be marked with the purchase date and the date opened e Peroxide forming solvents should be sealed tightly and stored away from light and heat e Periodic testing should be done on opened containers and the results marked on the containers Testing e Obtain test strips for the range of 0 100 ppm peroxide e Record the test results on the bottle e Ifthe test results are 100 ppm or greater contact REM 765 49 40121 for proper disposal 97 The official version of this document w
171. ure of elements and or compounds which is a physical or health hazard This definition of a hazardous chemical and the GHS primary classes of chemicals are briefly discussed below 3 1 Physical Hazards A chemical is a physical hazard if there is scientifically valid evidence that it is flammable combustible compressed gas explosive organic peroxide oxidizer pyrophoric self heating self reactive or water reactive Each physical hazard is briefly defined below Refer to Appendix B section B 1 for detailed information on each physical hazard e Explosives A liquid or solid which is in itself capable by chemical reaction of producing gas at such a temperature and pressure and at such a speed as to cause damage to the surroundings e Flammable Liquids Materials which under standard conditions can generate sufficient vapor to cause a fire in the presence of an ignition source and have a flash point no greater than 93 C 200 F e Flammable Solid A solid which is readily combustible or may cause or contribute to a fire through friction e Gases under Pressure Gases which are contained in a receptacle at a pressure not less than 280 kPA at 20 C or as a refrigerated liquid e Organic Peroxide A liquid or solid which contains the bivalent 0 0 structure and may be considered a derivative of hydrogen peroxide where one or both of the hydrogen atoms have been replaced by organic radicals e Oxidizer A liquid or solid while in its
172. zardous chemicals These requirements do not apply to labs that involve solely mechanical computer laser or other non ionizing radiation or electrical operations The requirements listed do not cover all operations in all laboratories Some operations and procedures may warrant further PPE as indicated by the SDS the SOP for the chemical s being used facility policies or regulatory requirements Figure 6 1 illustrates the minimum PPE required when using hazardous chemicals in a laboratory 63 The official version of this document will only be maintained online Chapter 6 Laboratory PPE Policy Safety Glasses or Goggles Lab Coat Chemical Resistant Gloves Long Pants Closed Toe Shoes Figure 6 1 Appropriate PPE for the Laboratory 6 4 1 Head Protection If there is a serious risk of chemical splash to the head a chemical resistant hoodie must be worn Each affected employee must wear protective helmets when working in areas where there is a potential for injury to the head from falling objects or bump hazards 6 4 2 Hearing Protection Hearing protection is not typically required in laboratory settings However if the lab seems excessively noisy e g operating equipment that is loud air handling unit is loud and it is difficult to communicate with co workers while in the lab contact REM 765 49 46371 fora noise level evaluation 64 The official version of this document will only be maintained online
173. zardous chemicals must be properly labeled by the chemical manufacturer or distributor before transportation occurs Chemical containers stored in laboratories are not required to be labeled per DOT standards however the DOT 9 hazard classes are often seen on chemical containers and are discussed in Section 14 of GHS formatted SDSs The DOT 9 hazard classes are illustrated below in Figure 2 5 It should be noted that Figure 2 5 only lists the primary hazard classes the sub classes e g Organic Peroxides DOT Class 5 2 were omitted for stylistic purposes weer HOW FLAMMABLE LAS eaten DOT Class 7 DOT Class 8 DOT Class 9 Radioactive Materials Corrosives Miscellaneous Figure 2 5 NFPA Hazard Rating System 15 The official version of this document will only be maintained online Chapter 2 Classes of Hazardous Chemicals Chapter 3 Classes of Hazardous Chemicals Chemicals can be divided into several different hazard classes The hazard class provides information to help determine how a chemical can be safely stored and handled Each chemical container whether supplied by a chemical manufacturer or produced in the laboratory must have a label that clearly identifies the chemical constituents In addition to a specific chemical label more comprehensive hazard information can be found by referencing the SDS for that chemical The OSHA Laboratory Standard defines a hazardous chemical as any element chemical compound or mixt

Chemical Hygiene Plan and Hazardous Materials Safety Manual

Contents

Download Pdf Manuals

Related Search

Related Contents