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G5664-90001 - Agilent Technologies

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1. CAN bus AC power Figure 8 Connections at the Rear of the Thermostatted Fraction Collector 1260 Infinity Bio inert Fraction Collector 39 3 Installing the Fraction Collector Flow Connections to the Fraction Collector Parts required Preparations Description Parts from the accessory kit Fraction Collector is installed in the LC system Explosive gas mixtures There is a risk of explosive gas mixtures in the instrument if flammable solvents are used gt Cover the plates gt Remove the plates from the fraction collector after turning it OFF gt Only use solvents with a flash point higher than 200 C Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere 40 1260 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 CAUTION Liquid spills or fraction losses Worn or damaged tubings can cause potential spills or lead to fraction
2. Procedure Typical Frequency Notes Exchanging the leak sensor When defective See Exchanging the Leak Sensor on page 157 Exchanging or installing the When defective or new board See Exchanging or Installing optional BCD board installed the Optional Interface Board on page 160 1260 Infinity Bio inert Fraction Collector 131 9 Maintenance Replacing the Inlet Waste Tubings When Parts required Preparations When contaminated worn out or visibly damaged Typically once every year p n Description G5664 68712 Analytical tubing kit 0 25 mm i d PTFE ESD Position the transport unit of the fraction collector in the Home position see Maintenance Functions on page 118 Remove all installed trays from the tray base Position the transport unit of the fraction collector in the Change Parts position see Maintenance Functions on page 118 Turn OFF the instrument Remove the rear end of the fraction collector s waste tubing from the waste container unscrew the front end of the fraction collector s inlet tubing from the flow cell of the detector Personal injury There is a risk of personal injury because the needle arm can move during fraction collector operation gt To avoid personal injury keep fingers away from the needle area CAUTION Liquid spills or fraction losses Worn or damaged tubings can cause potential spills or lead to fraction losses gt Explicitly fol
3. height x width x depth Line voltage Line frequency Power consumption Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC CSA UL 100 240 VAC 10 Wide ranging capability 50 or 60 Hz 5 180 VA 180 W 239 BTU Maximum 4 40 C 41 104 F 40 70 C 4 158 F lt 95 at 25 40 C 77 104 F Non condensing Up to 2000 m 6562 ft Up to 4600 m 15091 ft For storing the module Installation category II Pollution degree 2 For indoor use only 1260 Infinity Bio inert Fraction Collector 19 2 Site Requirements and Specifications Performance Specifications 20 Table3 Specifications Agilent 1260 Infinity Analytical Bio inert Fraction Collector G5664A Type Specifications Delay volume Maximum system flow Fraction containers Fraction trays Cooling Trigger modes Approximately 50 pL 10 mL min Shallow or deep well plates up to 48 mm height 96 or 384 format Test tubes up to 48 mm height Autosampler Vials 2 mL and 6 mL Eppendorf safe lock tubes 0 5 mL 1 5 mL 2 5 mL Full trays cover complete fraction collector 4 well plates 40 x 20 mL test tubes 30 mm od 48 mm height 60 x 15 mL test tubes 25 mm od 48 mm height 126 x 8 mL test tubes 16 mm od 48 mm height 215 x 5 mL test tubes 12 mm od 48 mm height Trays with possibiity to
4. The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch e The power input socket accepts a line voltage of 100 240 VAC 10 witha line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1260 Infinity Bio inert Fraction Collector Hardware Information 12 Electrical Connections Rear view of the module BOoooES amp Cem tanpoponoo Moots __ 400 63 3 Figure 35 Fraction Collector Electrical Connections 1260 Infinity Bio inert Fraction Collector 195 12 Hardware Information Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Ta
5. 26 If your fraction collector is part of a HPLC system you can ensure optimum performance and minimum delay volume by installing the following configuration Figure 1 on page 27 and Figure 2 on page 28 show the configuration recommended for the fraction collector in an analytical scale system Agilent 1260 Infinity LC Purification Systems can comprise many different LC modules This section describes some common stack configurations and general considerations On more complex systems e g with two or more fraction collectors or with mass selective detector other stack configurations might show advantages 1260 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 Optimizing the Stack Configuration Solvent cabinet oe e Pump S 900 000 888 Local user interface oe p Autosampler Thermostatted column compartment Detector Figure 1 Recommended Stack Configuration Front View 1260 Infinity Bio inert Fraction Collector 27 3 Installing the Fraction Collector Optimizing the Stack Configuration AC power CAN bus cable to local user interface Remote cable CAN bus cable LAN to control software location depends on detector EEEEELLJ Analog detector signal 1 or 2 outputs per detector Figure 2 Recommended Stack Configuration Rear View 28 12
6. CAUTION Loss of data loss of fractions With multiple fraction collectors a fraction start location must be specified Otherwise the fraction collection of the next run will start at the fraction collector where the last run stopped gt Always specify a fraction start location e g 1 P1 A 01 or 2 Vial 10 gt Never use the valve settings to define a start location 72 Sequence Parameters Instrument 1 x Operator Name Bio Chemist Data File Bar Code Reader Auto Prefix Counter T Use In Sequence Prefix Counter On a bar code mismatch fsict ooo Inject anyway Subdirectory PROTEOME Don t inject Path C HPCHEM 1 DATA Shutdown Part of methods to run According to Runtime Checklist F Post Sequence Cmdi Macra F Use Sequence Table Information macro SHUTDOWN MAC go WaitTime min nRdy Timeout min after loading a new method Fraction Information SS Fraction Start Location fi P1 A 01 gt Sequence Comment Cancel Help Figure 20 Start location in Sequence Parameters dialog box 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Starting Your Run with Fraction Collection To start a single run click Start in the graphical user interface of the ChemStation select Run Method from the RunControl menu press F5 To start the sequence click Start in the graphical user interface of the
7. Error ID 0070 Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line Defective remote cable Defective components in the instrument showing the not ready condition 1260 Infinity Bio inert Fraction Collector Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis Exchange the remote cable Check the instrument for defects refer to the instrument s documentation 101 7 Error Information Lost CAN Partner Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error
8. LoadSignal Ok Cancel Help Figure 17 Fraction Preview dialog box The Fraction Preview screen allows to test the fraction collection parameters against an example chromatogram It can also be used to optimize the fraction collection parameters interactively With the help of this tool values for up and down slope as well as for upper and lower threshold can easily be graphically specified To load a chromatogram e g a pilot run click Load Signal Parameters can now be changed either manually in the detector table and Timetable or graphically in the Fraction Preview screen By clicking the desired buttons on the right hand side of the Fraction Preview screen the chromatogram can be zoomed the values for up and down slope can be specified and the upper and lower threshold level can be set up The graphically specified values are automatically transferred to the Peak Detector table 68 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Starting Your Run with Fraction Collection Resetting the Current Fill Levels CAUTION To protect the collected fractions against contaminations and to avoid overfilling of single positions the fraction collector stores current fill levels It will block used positions if no fraction start location has been specified If a new tray of the same type is inserted in the fraction collector all fill levels can be reset not just the well plate vial or test tube After re
9. Note the orientation of the well plate The starting position A1 is always in the back left corner of the plate 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Sample Info Instrument 1 x Operator Name Bio Chemist Data File C Prefix Counter Manual Filename DEFAULT D Subdirectory PROTEOME Path C HPCHEM 1I DATA Location Vial 1 1 a Eee tun if no entry lt Eraction Start Location 1 P1 A 01 gt Sample Parameters Sample Name Sampie_o1 Plate I ID Sample Amount fo Multiplier 1 ISTD Amount fo Dilution f1 Comment Run Method Cancel Help Figure 19 Sample Info dialog box All settings made in the Sequence Table overrule the settings made in the Sequence Parameters screen In addition to the exact position for fraction collection start in the Sequence Table it is also possible to specify Next Plate and Next Location In the former case fraction collection is started at the next free plate and in the latter case fraction collection is started at the next free location If a fraction start location is specified the collector will start at this location even if this vessel has been used during a previous run If no fraction start location has been specified the fraction collection starts at the first empty vessel or location 1260 Infinity Bio inert Fraction Collector 71 4 Using the Fraction Collector Starting Your Run with Fraction Collection
10. 12 Start the instrument 13 Close the front cover 1260 Infinity Bio inert Fraction Collector 151 9 Maintenance Exchanging the Internal Tray Exchanging the Internal Tray When If defective Parts required p n Description G1364 63114 Internal tray analytical scale Preparations e Position the transport unit of the fraction collector in the Home position see Maintenance Functions on page 118 Remove all installed trays from the tray base Turn OFF the instrument 1 Locate the internal tray assembly with the rinse funnel 2 Remove the internal tray by pushing down the plastic and flow delay sensor in the bottom of the right front holder that holds it in position underneath the metal leash corner of the instrument 1 and sliding the tray to the left at the same time 2 152 1260 Infinity Bio inert Fraction Collector Maintenance 9 Exchanging the Internal Tray 3 Remove the corrugated waste tubing from the front of the 4 Install the new tray by sliding it into position underneath instrument 1 and slide the internal tray s waste tubing to the metal leash that holds it The waste tubing from the the rear of the outlet 2 the before sliding the tray out internal tray should be guided as shown 5 Make sure that the waste tubing is slid all the way Next Steps through the outlet 1 Its end should be over the edge and below the level of the laboratory desk that the sys
11. 1260 Infinity Bio inert Fraction Collector 215 13 Appendix Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren 216 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1260 Infinity Bio inert Fraction Collector Appendix 13 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that und
12. C and D can t be used A D F Rows A B C D and F can t be used Columns 1 Column 1 can t be used 1 2 Columns 1 and2 can t be used 1 4 Columns 1 2 3 and 4 can t be used 1 4 12 Columns 1 2 3 4 and 12 can t be used Single locations G12 H12 Locations G12 and H12 can t be used Configuring Multiple Fraction Collectors To increase the capacity of the systems up to three fraction collectors can be configured by using the Agilent 1260 Infinity Agilent 12 Position Selector Valve G1160A In addition a fourth fraction collector can be configured for recovery collection The Configuration has to be edited for all fraction collectors The last fraction collector in the configuration can be selected for sample recovery To configure the recovery fraction collector please read Sample Recovery on page 78 With multiple fraction collectors a fraction start location has to be specified to define the starting position For further details see Assignment of Start Location for Fraction Collection on page 70 Never use the valve settings to define a start location on systems with multiple fraction collectors We recommend to use inlet tubings of the same length for all fraction collectors Otherwise a Delay Calibration has to be performed for each of those fraction collectors For the recovery fraction collector the delay volume parameter will be ignored 1260 Infinity Bio inert Fraction Collector Using the Fraction Co
13. Card G1316A C TCC 2 No No Yes No Yes G1322A DEG No No No No No Yes AUX G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes G4227A Flex Cube 2 No No No No No G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED 1260 Infinity Bio inert Fraction Collector 197 12 Hardware Information The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN e CAN connectors as interface to other modules e LAN connector as interface to the control software e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosample
14. below the limit where this false triggering effect might occur As soon as the detector signal exceeds the Upper Threshold settings for Up Slope or Down Slope will be ignored until the signal drops again below the Upper Threshold 1260 Infinity Bio inert Fraction Collector 65 4 66 Using the Fraction Collector When using more than one peak detector fraction collection can be triggered either when all selected peak detectors detect a peak or when at least one selected peak detector detects a peak basing on the settings in the peak detectors table above If an MSD is used for mass based fraction collection Use MSD for mass based Fraction Collection must be checked Timetable The Timetable can be used to program changes in the Fraction Trigger Mode during the analysis by entering a Time and specifying the trigger settings Trigger Mode Off Peak Based and Time Based can be selected If the Off is selected no fractions are collected The last entry in the timetable has to be the command Off Whenever the Peak Based mode is specified fractions will be collected based on the peak detection parameters given in the Peak Detector table Additionally a Maximum Peak Duration in minutes has to be specified This parameter is mandatory if you use Peak Controlled fraction collection but is disabled for Time Based fraction collection When the Time Based mode is chosen two different options are available The of Fractions can be edit
15. carrier assembly 2 Bad needle carrier positioning in X or Theta 3 Disconnected needle carrier connector 4 Defective MTP main board 5 Defective Z motor Missing Vial or Missing Well plate Error ID Suggested actions Exchange the needle carrier assembly Perform a self alignment Connect needle carrier connector correctly Please contact your Agilent service representative Please contact your Agilent service representative No vial or well plate was found in the position defined in the method or sequence When the needle carrier moves to a vial or well plate and the needle is lowered into the vial or well plate the position of the needle is monitored by an encoder behind the vial pusher If no vial or well plate is present the encoder detects an error and the message missing vial or well plate is generated Probable cause 1 No vial in the position defined in the method or sequence 2 Defective needle carrier assembly 3 Defective transport unit assembly flex board 4 Defective MTP main board 1260 Infinity Bio inert Fraction Collector Suggested actions Install the sample vial in the correct position or edit the method or sequence accordingly Exchange the needle carrier assembly Please contact your Agilent service representative Please contact your Agilent service representative 109 7 Error Information Calib delay vol two peaks Error ID 4759 Two peaks have been
16. gt Keep fingers away from the needle area during fraction collector operation Do not attempt to insert or remove a vial or a plate when the needle is positioned 30 1260 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 WARNING The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed 1 Install the LAN interface board in the fraction collector if required see Exchanging or Installing the Optional Interface Board on page 160 Remove the adhesive tape which covers the side and front doors Open the front door and remove the left side door Remove the transport protection foam oF Ww N Install the corrugated waste tube in the plastic port at the front bottom center of the instrument Slide the waste tubing coming from the internal tray if present through the plastic port and the corrugated waste tube see 1260 Infinity Bio inert Fraction Collector 31 3 Installing the Fraction Collector Figure 3 on page 32 Route the corrugated waste tubing into a waste container Figure 3 Installing the Corrugated Waste Tubing in the Plastic Port 6 Re install
17. if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1260 Infinity Bio inert Fraction Collector In This Guide In This Guide This manual contains technical reference information about the Agilent 1260 Infinity Bio inert Analytical scale Fraction Collector G5664A 1 Introduction This chapter gives an introduction to the module 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Fraction Collector This chapter provides information on unpacking checking on completeness stack considerations and installation of the module 4 Using the Fraction Collector This chapter provides information on how to set up the module 5 How to Optimize the Fraction Collector This chapter provides information on how to optimize the module 6 Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic features 7 Error Information This chapt
18. leak sensor and its cable from the instrument 4 Bend the new leak sensor so that it has a 90 angle to its plastic holder as shown The bend must be sharp enough that the sensor reaches all the way down to the bottom of the leak plane but the cables must not be damaged 5 Install the new leak sensor into its position in the leak plane 6 Route the leak sensor cable nicely into the recess at the front of the instrument Plug the leak sensor into its connector on the SLF board 158 1260 Infinity Bio inert Fraction Collector Maintenance Exchanging the Leak Sensor 7 Re install the front tray guide 3 screws Be careful not to pinch the new leak sensor s cable f 3 screws Next Steps 8 Re install the tray s in the tray base 9 Start the instrument 10 Close the front cover 1260 Infinity Bio inert Fraction Collector 159 9 Maintenance Exchanging or Installing the Optional Interface Board When At installation when defective or for all repairs inside the fraction collector Tools required Description Screwdriver flat head CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use ESD protection for example an ESD wrist strap when handling elect
19. 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 1260 Infinity Bio inert Fraction Collector Identifying Cables 11 RS 232 Cable Kit p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m 1260 Infinity Bio inert Fraction Collector 191 11 Identifying Cables RS 232 Cable Kit 192 1260 Infinity Bio inert Fraction Collector 1260 Infinity Bio inert Fraction Collector eece 12 7 e Hardware Information e i e Electrical Connections 194 Rear view of the module 195 Interfaces 196 Overview Interfaces 198 Setting the 8 bit Configuration Switch without On board LAN 202 Communication Settings for RS 232C 203 Special Settings 205 Optional Interface Boards 206 This chapter describes the module in more detail on hardware and electronics gg Agilent Technologies 193 12 Hardware Information Electrical Connections 194 e The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems
20. ChemStation select Run Sequence from the RunControl menu press F6 Online Tick Marks To display tick marks for fraction start and stop events in you Online Plot click Change in your Online Plot window Then check Show fraction collection ticks in the Edit Signal Plot window Figure 21 on page 73 Fie RunControl Inctrument Method Sequence Purfy View Abert Hep i ell A ai ecKouras m x Sequences IAR id Selected Snel paa Spade 24 16 Rel erence 340 JB Saale 254 16 References gt C Signale210 8 Referenced On Sagnade 230 16 References r ae Matthod Settings 1 Show frachon collection che I Use method settings Apply to Method Ox Cancel Help coi tan e eee eee TD 26 3 346 4 45 6 ce O min 4 Change N Adjust Balance m0 Ready momin Figure 21 Online tick marks 1260 Infinity Bio inert Fraction Collector 73 4 Using the Fraction Collector Viewing Your Results Data Analysis In order to display the tick marks for the collected fractions on the screen click Signal options from the Graphics menu Then choose Separated in the Layout dropdown list To review your chromatograms file information and a fraction list select the Data Analysis view and click Fraction Task as displayed below 74 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Viewing Your Results Chromatogram with Fraction Tick Marks Purification Ta
21. G1364 84521 gt Remove all wellplates 120 1 Ifthe transport unit has been exchanged or if it is strongly misaligned set the 8 bit configuration switch to the Forced Cold Start Configuration see Table 26 on page 205 2 Install 4 well plate tray p n G1364 84521 Remove all plates 3 Ensure that the wellplate adapter is correctly assembled 4 Select the Maintenance menu in the Diagnosis view of the Agilent ChemStation 1260 Infinity Bio inert Fraction Collector Test Functions 8 5 In the menu choose Fraction Collector gt Transport Alignment to start the automated procedure The Transport Alignment Procedure takes approximately 10 15 minutes 6 Set the 8 bit configuration switch to the default setting see Table 20 on page 208 If the Transport Unit Self Alignment is started with well plates on the tray the alignment procedure is aborted without any error message 1260 Infinity Bio inert Fraction Collector 121 Test Functions Step Commands 122 Some movements of the fraction collection sequence can be done under manual control This is useful during troubleshooting where close observation of each of the fraction collection step is required to confirm a specific failure mode or verify successful completion of a repair Each step command actually consists of a series of individual commands which move the fraction collector components to predefined positions enabling the specific step to be done In
22. Lab Advisor the step commands can be selected from the Tools screen Automatic Fraction Collector Steps Valve to Waste Valve to Vessel Needle Up Move to Location Vial Move to Rinse Needle into Vessel Figure 28 Fraction Collector Step Commands 1260 Infinity Bio inert Fraction Collector Test Functions 8 Table 15 Step Commands Step Action Comments Needle Up Lifts the needle arm to the Command also switches the upper position diverter valve to waste if it is not already in that position Needle into vessel Lowers the needle into the specified vessel Needle to rinse flush port Moves the needle to the rinse flush port Switch valve to needle Switches the diverter valve to the needle Switch valve to waste Switches the diverter valve from needle to waste Troubleshooting If the fraction collector is unable to perform a specific step due to a hardware failure an error message is generated You can use the step commands to perform a fraction collection sequence and observe how the fraction collector responds to each command 1260 Infinity Bio inert Fraction Collector 123 8 Test Functions Step Commands 124 1260 Infinity Bio inert Fraction Collector 1260 Infinity Bio inert Fraction Collector Ogee Maintenance Introduction to Maintenance 126 Cautions and Warnings 127 Cleaning the Module 129 Overview of Maintenance 130 Replacing the Inlet Waste Tubings 132 Replacing the Valve to N
23. Please contact your Agilent service representative Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 1260 Infinity Bio inert Fraction Collector Error Information 7 Leak Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries Open Cover Error ID 0205 The top foam has been removed Probable cause Suggested actions 1 Foam not activating the sensor Please c
24. Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false e fan out is 10 1260 Infinity Bio inert Fraction Collector Hardware Information 12 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Table 19 Remote Signal Distribution Pin S
25. a metal free flow path for the sample The pH range is from 1 13 short term 14 pH Bio inert Materials 10 For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality materials in the flow path also referred to as wetted parts which are widely accepted by life scientists as they are known for optimum inertness to biological samples and ensure best compatibility with common samples and solvents over a wide pH range Explicitly the complete flow path is free of stainless steel and free of other alloys containing metals such as iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction contains no metals whatsoever Table 1 Bio inert Materials Module Materials Agilent 1260 Infinity Bio inert Quaternary Pump Titanium gold platinum iridium G5611A ceramic ruby PTFE PEEK Agilent 1260 Infinity Bio inert High Performance Autosampler Upstream of sample introduction G5667A e Titanium gold PTFE PEEK ceramic Downstream of sample introduction PEEK ceramic Agilent 1260 Infinity Bio inert Manual Injector PEEK ceramic G5628A Agilent 1260 Infinity Bio inert Analytical Fraction Collector PEEK ceramic PTFE G5664A 1260 Infinity Bio inert Fraction Collector Introduction 1 Table 1 Bio inert Materials Module Materials Bio inert Flow Cells Standard flow cell bio inert 1
26. a multiple fraction collector configuration the function rinse between fractions is ignored Auxiliary In the Auxiliary section the Maximum fill volume per location can be specified If as configured is selected the pre configured volume see Instrument gt Pre configured Wellplate Types is used This ensures that the location well vial or tube cannot be overfilled during fraction collection This volume can be further reduced by defining a customized volume Additional check boxes in this section provide the opportunity to Store temperature signal and to Store UIB signal Fraction Preview To determine the appropriate fraction collection parameters the Agilent ChemStation provides a valuable tool that becomes accessible by pushing the button labelled Fraction Preview Tool Figure 17 on page 68 in the Peak Detectors section 1260 Infinity Bio inert Fraction Collector 67 4 Using the Fraction Collector Fraction Preview PeakBased p p A x r Peak Detectors Detector Working Mode Up Slope Unit s Down Slope Unit s Threshold Unit Upper Threshold Unit Unit 1 DADI Threshold Slope so so 000 3000 000 2 UIB Off 5 00 5 00 5 000 3000 000 mV m Timetable Insert Time Trigger Mode Max Peak Duration min of Fractions Timeslices min Append Cut Copy Paste Refresh Fraction Preview DAD1 A Sig 254 4 Ref 550 100 DEMOW05 0101 D Threshold
27. at the front of the module is switched on and an entry is written into the module logbook 1260 Infinity Bio inert Fraction Collector 99 7 Error Information General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required 100 1260 Infinity Bio inert Fraction Collector Shutdown Error ID 0063 Error Information 7 An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system Leak detected in an external instrument with a remote connection to the system Shut down in an external instrument with a remote connection to the system Remote Timeout
28. by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up WARNING es alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 214 1260 Infinity Bio inert Fraction Collector Appendix 13 The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information
29. cover 1260 Infinity Bio inert Fraction Collector 139 9 Maintenance Exchanging the Needle Assembly When When the needle is visibly damaged When the needle is blocked or contaminated Tools required p n Description 8710 1534 Wrench open end 4 mm 8710 0510 Wrench open 1 4 5 16 inch Parts required p n Description G5667 87200 Needle assembly bio inert Preparations Position the transport unit of the fraction collector in the Home position see Maintenance Functions on page 118 Remove all installed trays from the tray base Position the transport unit of the fraction collector in the Change Parts position see Maintenance Functions on page 118 and turn off the instrument It might be more convenient to remove the needle from its carrier before unscrewing the needle tubing WARNING ie There is a risk of personal injury because the needle arm can move during fraction collector operation gt To avoid personal injury keep fingers away from the needle area 140 1260 Infinity Bio inert Fraction Collector Maintenance 9 Exchanging the Needle Assembly 1 Use the 4 mm wrench and the 5 16 wrench for counter holding to unscrew the valve to needle tubing from the needle assembly Viewed from the bottom 1260 Infinity Bio inert Fraction Collector 2 Remove the wellplate adapter Hold the needle assembly between your thumb and forefinger slide out the assembly towa
30. done on a more frequent basis Table 16 Simple Repair Procedures Procedure Typical Frequency Notes Replacing the inlet waste tubings Replacing the valve to needle tubings Exchanging the needle assembly Exchanging the needle capillary carrier assembly Exchanging the diverter valve Exchanging the internal tray Repairing or exchanging a funnel of the internal tray or funnel tray When worn out when showing visual signs of damage typically once per year When worn out when showing visual signs of damage typically once per year When needle shows indication of damage or blockage Or when using the short needle assembly for operation with high test tubes 45 mm gt When the needle carrier is defective When defective internal external leak valve not switching any more When flow delay sensor defective When defective leaky blocked or contaminated See Replacing the Inlet Waste Tubings on page 132 See Replacing the Valve to Needle Tubing on page 136 See Exchanging the Needle Assembly on page 140 See Exchanging the Needle Capillary Carrier Assembly on page 143 See Exchanging the Diverter Valve on page 148 See Exchanging the Internal Tray on page 152 See Repairing or Exchanging a Funnel of the Internal Tray on page 154 1260 Infinity Bio inert Fraction Collector Maintenance 9 Table 16 Simple Repair Procedures
31. gt To prevent personal injury be careful when getting in contact with sharp metal areas Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere 1260 Infinity Bio inert Fraction Collector 127 9 Maintenance CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use ESD protection for example an ESD wrist strap when handling electronic boards and components CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment CAUTION Sample degradation and contamination of the instrument Metal parts in the flow path can interact with the bio molec
32. installation of the tray a pop up window see Figure 18 on page 69 will be displayed Click Yes to reset all fill levels from the previous run G Tray 1 was replaced by a tray of the same type 7 Do you want to reset the Fill volume ves No Figure 18 Reset fill volumes Contaminated fractions unwanted overfill events or split fractions The window Figure 18 on page 69 to reset current fill levels is not displayed if the well plate the vial or the test tube is removed without removing the complete tray Consequently contaminated fractions unwanted overfill events or split fractions might be observed during the following LC run gt Remove the complete tray to correctly reset fill levels 1260 Infinity Bio inert Fraction Collector 69 4 70 Using the Fraction Collector Assignment of Start Location for Fraction Collection The start location for fraction collection can either be assigned in the Sample Info dialog box Figure 19 on page 71 in the Run Control menu in the Sequence Parameters dialog box Figure 20 on page 72 or in the Sequence Table in the Sequence menu All fraction start locations are entered in the following formats For vials and test tubes fraction collector vessel number e g 1 Vial 1 For well plates fraction collector plate number row column e g 1 P1 A 01 with fraction collector 1 2 plate number P1 P4 row is A to H and column 1 12 for 96 well plates
33. instruction given in Lab Advisor 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Setting up a Fraction Collector Method In order to setup or change the method parameters of your fraction collector select Setup Fraction Collector from the Instrument menu or right click on the fraction collector icon in the graphical user interface This will open the Setup Fraction Collector dialog box as displayed in Figure 15 on page 63 In the Setup Fraction Collector dialog box general method settings are specified Set up Fraction Collector LC113 xi Fraction Trigger Mode Off Use Timetable Max Peak Duration 0 5 min Peak Detectors Detector Working Mode Up Slope Unit s Down Slope Unit s Threshold Unit Upper Threshold Unit Unit 1 DADI Threshold Slope 5 00 10 00 10 000 3000 000 mAU Fraction is collected when a peak is detected by F Use MSD for Mass based Fraction Collection all selected peak detectors at least one selected peak detector Timetable Time Trigger Mode Max Peak Duration min of Fractions Timeslices min Append Time Rinse Fraction Collection Needle Auxiliary Max fill volume per location Stoptime pis a min I At the start of collection as configured a ml Posttime Off min I Between fraction collection I Store temperature signal Store delay sensor signal F Store UIB signal Figure 15 Set up Fraction Collector dialog box 1260
34. losses gt Explicitly follow the described installation procedures to maximize the lifetime of inlet waste tubing assembly and the valve to needle tubing and to avoid potential spills or fraction losses gt Regularly inspect the tubings and exchange them if they are worn out or show visible signs of damage To waste From flow cell T Figure 9 Flow Connections to and from the Fraction Collector 1 Route the waste outlet around the instrument as shown below and into a corrugated waste tubing Fix the waste outlet tubing and the corrugated waste tubing to the instrument with the sticking holders part of the 1260 Infinity Bio inert Fraction Collector 41 3 42 Installing the Fraction Collector Flow Connections to the Fraction Collector Accessory Kit p n G1364 68755 as shown below Route the corrugated waste tubing into a waste container Figure 10 Fixing and Routing the Waste Tubings 1260 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 Fraction Collector Trays Installing the Fraction Collector Trays WARNING Explosive gas mixtures There is a risk of explosive gas mixtures in the instrument if flammable solvents are used gt Cover the plates gt Remove the plates from the fraction collector after turning it OFF gt Only use solvents with a flash point higher than 200 C WARNING Contamination with adhesives Closing mats with adhesives
35. of the collected liquid This option is recommend for low flow rates and small fraction volumes to avoid air bubbles and accomplish an accurate fraction volume Well Plates In the Well Plates section the type of well plates used in a well plate tray can be configured The well plate type can be chosen from the Plate Type dropdown list More detailed information about all pre configured well plates can be found in the Instrument menu Only one type of well plate can be used on the well plate tray The checkbox Treat as 4 x 96 is available only for 384 well plates and allows to split the 384 well plate virtually into 4 separate 96 well plates This only changes the collection order The numbering of the wells remains as indicated on the plate The four virtual 96 well plates start at locations Al A13 I1 and 113 respectively The filling order of each quarter is as specified in the Collection Order section When the 384 well plate is split into four equal quarters the order of the four plates is the same as displayed in the Installed Plates section In the Reserved Locations you have the possibility to specify locations that will not be used for Fraction Collection see Table 7 on page 56 1260 Infinity Bio inert Fraction Collector 55 4 56 Using the Fraction Collector Table 7 Syntax for the definition of Reserved Locations Location Syntax Description Rows A Row A can t be used A B Rows A and B can t be used A D A B
36. performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1260 Infinity Bio inert Fraction Collector Introduction 1 Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations e the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio freque
37. right click on the fraction collector icon in the graphical user interface In the resulting Fraction Collector Configuration dialog box Figure 13 on page 53 the configuration of the Trays the Fraction Delay Volumes the Collection Order the Needle Movement and the Well Plates can be specified Trays In the online mode of the Agilent ChemStation the type of the installed tray is recognized automatically In the off line mode an appropriate tray type can be chosen from a dropdown list For a selection of supported trays see Supported Trays for a Fraction Collector on page 166 Tube volume ml Volume of the test tube should be specified 10 less than the maximum fill volume to avoid overfill Tube height mm The exact test tube height has to be specified For the analytical scale fraction collector with 50 mm needle standard the maximum height is 48 mm In the online ChemStation the installed tray is recognized and the default settings for tube volume and tube height are loaded and displayed 52 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Fraction Collector Configuration LC113 Trays Tray A 40 Tubes Tray B Tube Volume ml 45 00 Tray C Tube Height mm 38s Fraction Delay Volumes Collection Order Detector _ Yolume u C Row by row gt Shortest path 1 DADI 500 by column r m Fra Collection Mode Needle Movement P L 1B aae MAAE int
38. supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment 1260 Infinity Bio inert Fraction Collector 17 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 2 on page 19 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 18 1260 Infinity Bio inert Fraction Collector Physical Specifications Site Requirements and Specifications 2 Table2 Physical Specifications Type Specification Comments Weight 13 5 kg 29 8 lbs Dimensions 200 x 345 x 440 mm 8 x 13 5 x 17 inches
39. to hydrochloric or sulfuric acids significantly reduces corrosion rates Titanium is subject to corrosion in anhydrous methanol which can be avoided by adding a small amount of water about 3 Slight corrosion is possible with ammonia gt 10 Fused silica Fused silica is inert against all common solvents and acids except hydrofluoric acid It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with sapphire windows is recommended 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Gold Gold is inert to all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia a mixture of concentrated hydrochloric and nitric acid Zirconium Oxide Zirconium Oxide ZrOg is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications PTFE PTFE polytetrafluorethen is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Sapphire Ruby and Al 03 based ceramics Sapphire ruby and cerami
40. transport unit of the fraction collector in the Change Parts position see Maintenance Functions on page 118 and turn off the instrument It might be more convenient to remove the needle from its carrier before unscrewing the needle tubing WARNING ie There is a risk of personal injury because the needle arm can move during fraction collector operation gt To avoid personal injury keep fingers away from the needle area CAUTION Liquid spills or fraction losses Worn or damaged tubings can cause potential spills or lead to fraction losses gt Explicitly follow the described installation procedures to maximize the lifetime of inlet waste tubing assembly and the valve to needle tubing and to avoid potential spills or fraction losses gt Regularly inspect the tubings and exchange them if they are worn out or show visible signs of damage 136 1260 Infinity Bio inert Fraction Collector Maintenance 9 Replacing the Valve to Needle Tubing 1 Locate the diverter valve with the finger tight fittings of the valve to needle tubing assembly the figure shows the open fraction collector seen from the front 2 Unscrew the finger tight fitting of the valve to needle tubing assembly at the diverter valve ee io d all Tosh L pi A ALF 3 Using the 4 mm wrench and the 5 16 wrench for counter holding unscrew the va
41. valve 5 Using the 2 mm hex key loosen the holding screw of the diverter valve and remove the diverter valve from the z arm 4 Disconnect the power cable of the diverter valve from the connector on the front of the z arm 6 Install the new diverter valve to the z arm with its holding screw Plug in the new diverter valve power cable to the z arm 1260 Infinity Bio inert Fraction Collector 149 9 Maintenance Exchanging the Diverter Valve 7 Install the valve to needle tubing at the new diverter valve 8 Connect the finger tight fittings of the inlet waste tubing assembly to the ports of the diverter valve View from the bottom SA gt me Ai S HN AS lt a e et mN Z SZ aA n Sm 4 Ae Lt By Zo 5 NOTE The tubings must not be bent up or downwards The cables must not be twisted 9 Acolor coded ring on one of the tubings and the valve body indicates which cable belongs to which port 1260 Infinity Bio inert Fraction Collector Maintenance 9 Exchanging the Diverter Valve NOTE 10 IMPORTANT The cables must run into the ports of the s diverter valve in lines parallel to the horizon It is absolutely vital to connect these tubings as described in order to maximize their lifetime and operating security Next Steps 11 Re install the tray s in the tray base
42. 0 mm 13 pl 120 bar 12 MPa PEEK ceramic sapphire PTFE for MWD DAD includes Capillary Kit Flow Cells BIO p n G5615 68755 p n G5615 60022 for Agilent 1260 Infinity Diode Array Detectors DAD G1315C D Max Light Cartridge Cell Bio inert 10 mm V o 1 0 pl p n PEEK fused silica G5615 60018 and Max Light Cartridge Cell Bio inert 60 mm V o 4 0 pl p n G5615 60017 for Agilent 1200 Infinity Series Diode Array Detectors DAD G4212A B Bio inert flow cell 8 uL 20 bar pH 1 12 includes Capillary Kit PEEK fused silica PTFE Flow Cells BIO p n G5615 68755 p n G5615 60005 for Agilent 1260 Infinity Fluorescence Detector FLD G1321B Bio inert heat exchanger G5616 60050 for 1290 Infinity PEEK steel cladded Thermostatted Column Compartment G1316C Bio inert Valve heads G4235A G5631A G5639A PEEK ceramic Al 03 based Bio inert Connection capillaries Upstream of sample introduction Titanium Downstream of sample introduction Agilent uses stainless steel cladded PEEK capillaries which keep the flow path free of steel and provide pressure stability to more than 600 bar To ensure optimum bio compatibility of your Agilent 1260 Infinity Bio inert LC system do not include non inert standard modules or parts to the flow path Do not use any parts that are not labeled as Agilent Bio inert For solvent compatibility of these materials see Solvent information for parts of the 1260 Infinity Bio ine
43. 1 B23 With the 2 plates 10 x 2ml vials or 10 funnels std trays e Plate in the front position P1 e Plate in the back position P2 Vessel Al AQ B1 B23 e Vials funnels 1 10 With the 100 vials std tray e Vial 1 100 1260 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 With the half trays Left hand 40 position tray 1 40 e Center 40 position tray 101 140 e Right hand 40 position tray 201 240 or Left hand 15 position tray 1 15 e Center 15 position tray 101 115 e Right hand 15 position tray 201 215 With the 40 60 125 or 215 position test tube full trays e Numbering starts in front left corner in columns to the back and then to the right 1260 Infinity Bio inert Fraction Collector 45 3 Installing the Fraction Collector ell Configure plate Types Configure Well plate Types If the plate you are using is not found on the List of Recommended Plates and Closing Mats on page 171 you may configure a custom plate Measure the exact dimensions of the plate as marked below and enter the values in the plate configuration table of the ChemStation or the Agilent Instant Pilot G4208A column Figure 11 Well plate Dimensions straight 46 1260 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 Figure 12 Well plate Dimensions staggered Table 6 Configuring Well plate Types Locatio
44. 11 Identifying Cables Cable Overview 180 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n Description 35900 60750 Agilent module to 3394 6 integrators 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs Remote cables p n Description 03394 60600 Agilent module to 3396A Series integrators 3396 Series Il 3395A integrator see details in section Remote Cables on page 184 03396 61010 Agilent module to 3396 Series III 3395B integrators 5061 3378 Remote Cable 01046 60201 Agilent module to general purpose BCD cables p n Description 03396 60560 Agilent module to 3396 integrators G1351 81600 Agilent module to general purpose 1260 Infinity Bio inert Fraction Collector Identifying Cables 11 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n Description G1103 61611 External contact cable Agilent module interface board to general purposes RS 232 cables p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5
45. 4 IMPORTANT After fixing the needle in the needle carrier clip the tubing into the guide on the bottom of the needle carrier assembly in case it slipped out of this guide It is absolutely vital that the tubing is installed as described to maximize the lifetime of the tubing 15 Re install the tray s in the tray base 16 Start the instrument 17 Close the front cover 1260 Infinity Bio inert Fraction Collector 147 9 Maintenance Exchanging the Diverter Valve Exchanging the Diverter Valve When If leaky or defective Tools required p n Description 8710 2438 Hex key 2 0 mm Parts required p n Description G5664 60901 3 2 Way valve assembly bio inert Preparations e Position the transport unit of the fraction collector in the Home position see Maintenance Functions on page 118 Remove all installed trays from the tray base Turn OFF the instrument 1 Locate the diverter valve with the finger tight fittings of 2 Unscrew the two finger tight fittings of the inlet waste the inlet waste tubing assembly the figure shows the tubing assembly at the diverter valve open fraction collector seen from the front 148 1260 Infinity Bio inert Fraction Collector Maintenance 9 Exchanging the Diverter Valve 3 Unscrew the finger tight fitting of the valve to needle tubing assembly at the diverter
46. 60 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 If a G1330B ALS thermostat is part of the system it must always be installed underneath the thermostatted injector or fraction collector see Installing a Thermostatted Fraction Collector on page 35 The fraction collector should never be positioned on top of a module that generates heat This could lead to an unwanted evaporation of fractions in the fraction collector e g Agilent 1260 Infinity Thermostatted Column Compartment G1316A or Agilent 1260 Infinity Diode Array Detectors 1260 Infinity Bio inert Fraction Collector 29 3 Installing the Fraction Collector Installing the Module Parts required Preparations Description Fraction Collector Chemstation or Agilent Instant Pilot G4208A Power cord For other cables see below and Cable Overview on page 180 Locate bench space Provide power connections Unpack the fraction collector When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Personal injury In the needle area there is a risk of beeing hurt by the needle during operation
47. 60 Infinity Bio inert Fraction Collector 115 7 Error Information Module Error Messages 116 1260 Infinity Bio inert Fraction Collector 1260 Infinity Bio inert Fraction Collector I hd Test Functions e E e j Maintenance Functions 118 Transport Unit Self Alignment 120 Step Commands 122 Troubleshooting 123 This chapter describes the module s built in test functions gy Agilent Technologies 117 Test Functions Maintenance Functions 118 Some maintenance procedures require the needle arm and needle carrier to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position In the ChemStation the Fraction Collector Maintenance Positions can be selected from the Maintenance menu in the Diagnosis view see Figure 27 on page 118 In the Instant Pilot the functions can be selected in the Test screens of the fraction collector Fraction collector Maintenance Positions Change Parts End Move Arm Home Park Arm for Transport Park Arm m Figure 27 Fraction Collector Maintenance Positions dialog box Change Parts If you click Start the transport unit will move upwards the needle carrier assembly will move to the front center and then turn off the theta motor to allow free rotation of the theta arm This position enables easy access to the transport unit to e ch
48. Agilent 1260 Infinity Bio inert Analytical scale Fraction Collector User Manual Notices Agilent Technologies Inc 2011 2012 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 65664 90001 Rev B Edition 05 2012 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information
49. Collector 52 Delay Volumes and Delay Calibration 58 Setting up a Fraction Collector Method 63 Starting Your Run with Fraction Collection 69 Viewing Your Results 74 Special Applications 77 Limitations and How to Avoid Problems 80 Check out Procedures 81 Application Notes 84 Solvent Information 85 1260 Infinity Bio inert Fraction Collector Contents How to Optimize the Fraction Collector 89 Optimizing Fraction Collection 90 Troubleshooting and Diagnostics 91 Overview of the Module s Indicators and Test Functions 92 Status Indicators 93 Agilent Lab Advisor Software 95 Error Information 97 What Are Error Messages 99 General Error Messages 100 Module Error Messages 106 Test Functions 117 Maintenance Functions 118 Transport Unit Self Alignment 120 Step Commands 122 Maintenance 125 Introduction to Maintenance 126 Cautions and Warnings 127 Cleaning the Module 129 Overview of Maintenance 130 Replacing the Inlet Waste Tubings 132 Replacing the Valve to Needle Tubing 136 Exchanging the Needle Assembly 140 Exchanging the Needle Capillary Carrier Assembly 143 Exchanging the Diverter Valve 148 Exchanging the Internal Tray 152 Repairing or Exchanging a Funnel of the Internal Tray 154 Exchanging the Leak Sensor 15 7 Exchanging or Installing the Optional Interface Board 160 Replacing Module Firmware 162 1260 Infinity Bio inert Fraction Collector Contents 10 Parts for Maintenance 163 Fraction Collector M
50. Halftray for 40 x 2 mL vials p n G1313 44512 In this configuration the forty 2 ml vials on the half will automatically be used for recovery b Standard tray for two well plates 10 x 2 ml vials p n G2258 60011 and Halftray for 15 x 6 mL vials p n G1313 44513 In this configuration the fifteen 6 mL vials on the half will automatically be used for recovery c Standard tray for two well plates 10 x 2 ml vials p n G2258 60011 and Half Tray for 40 Funnels p n G1364 84532 In this configuration the funnels on the half will automatically be used for recovery 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 The Std tray for 2 well plates 10 collecting funnels p n G1364 84522 allows two modes of operation a If well plates are configured in the fraction collector configuration the checkbox Plate 1 or Plate 2 are selected the 10 funnel locations will automatically be used for recovery b Ifthe checkbox Plate 1 and Plate 2 are not selected the 10 funnel position can be used for regular fraction collection CAUTION Fraction contamination With each start of a new sequence the recovery will start at the same position This can lead to fraction contamination if the vessels containing the recovery fractions are not exchanged gt Exchange the vessels containing the recovery fractions before starting a new sequence The number of recovery locations automatically defines the maximum number of
51. Infinity Bio inert Fraction Collector 63 4 64 Using the Fraction Collector Fraction Trigger Mode Use Timetable Enables the Timetable Peak based If Peak based is selected the collection of a fraction is triggered by the signal of the detector e g the Agilent 1260 Infinity Diode Array Detector or Variable Wavelength Detector The detailed trigger conditions are specified in the Peak Detectors table In the peak based trigger mode all entries in the timetable are ignored Max Peak Duration Defines a maximum collection time in case that the signal does not reach the condition to cut the fraction as exhibited in Figure 16 on page 64 This could be caused by tailing peaks or if the baseline is drifting during gradient runs The default value is set to 0 5 minutes If broad peaks are expected this value should be increased without exceeding the run time Threshold Max peak duration Figure 16 Maximum Fraction Duration 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Peak Detectors In the Peak Detectors section a list of all peak detectors that are connected to the system is displayed Agilent 1260 Infinity diode array detectors multiwavelength detectors variable wavelength detectors and fluorescence detectors are recognized automatically Other detectors e g Agilent 6000 mass selective detectors or HP1050 detectors are connected through the Universal Interface Box UIB The peak detect
52. The processor monitors the current drawn by each motor and the time the motor is drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the time the motor draws current is too long the error message is generated Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 High friction in the transport assembly Please contact your Agilent service representative 3 Motor belt tension too high Switch off the module at the power switch Wait at least 10 minutes before switching on again 4 Defective motor Please contact your Agilent service representative 5 Defective transport assembly flex board Please contact your Agilent service representative 1260 Infinity Bio inert Fraction Collector 113 7 Error Information Vessel Stuck to Needle Error ID 4453 The vessel sticks to the needle when the needle moves up Probable cause Suggested actions 1 Closing mat to rigid thick Check that the closing mat is not too thick 2 Bad X or Theta positioning and the needle Please contact your Agilent service sticks into the wall between two holes representative 3 Defective encoder on the needle carrier Please contact your Agilent service assembly representative Cluster Partner Lost During Analysis Error ID There was a problem with the inter module communication Pro
53. V The Agilent 1260 Infinity Fraction Collector has been installed and configured The delay volume has been calibrated as described in Performing a Delay Calibration with an UV Detector on page 62 V The 40 vial tray Halftray for 40 x 2 mL vials p n G1313 44512 with at least four 2 ml vials is installed V All wells are empty and the fill levels which are stored on the module have been reset To verify this remove and insert the 40 vial tray Then click Yes in the displayed dialog box 1260 Infinity Bio inert Fraction Collector 81 4 Using the Fraction Collector Table 13 Method Parameters for UV triggered Fraction Collection Check out AS Agilent 1260 Infinity modules Standard or micro degasser lsocratic quaternary or binary pump Autosampler well plate sampler Column compartment Diode array detector multi wavelength detector or variable wavelength detector with analytical flow cell e Fraction collector analytical scale Column Zorbax Eclipse XDB C18 150 mm x 4 6 mm 5 pm p n 993967 906 or equivalent Sample Agilent isocratic checkout sample p n 01080 68704 Flow 0 8 mL min Stoptime 6 minutes Solvent A 10 HPLC grade water Solvent B 90 HPLC grade acetonitrile Wavelength VWD 254 nm Wavelength DAD MWD Signal A 254 16 nm Reference 360 80 nm Peakwidth Responsetime gt 0 1 min 2 s Injection volume Tul Column temperature 40 0 C Fraction trigger mode Peak based Max peak duration 0 5
54. _Diverter Valve Assembly 1260 Infinity Bio inert Fraction Collector 175 10 Parts for Maintenance Tubing Kits Tubing Kits Item p n Description G5664 68712 Analytical tubing kit 0 25 mm i d PTFE ESD 1 G5664 86706 Inlet waste tubing assembly analytical scale 0 25 mm i d 2 G5664 86703 Diverter Valve to needle tubing analytical scale 0 25 mm i d 3 0100 1516 Fitting male PEEK 2 pk 3 gt m EER HO r S sgi oomunn H01 ac SI Hoi Figure 33 Tubing kits 176 1260 Infinity Bio inert Fraction Collector Internal Tray Assembly Item p n 1 G1364 63114 2 5022 2200 3 G1364 68730 4 G1364 86708 5 G1364 43201 Parts for Maintenance 10 Internal Tray Assembly Description Internal tray analytical scale Funnel Funnel seal kit pack of 10 Waste tubing kit 0 5T analytical scale Funnel coupler Flow delay sensor not shown Figure 34 1260 Infinity Bio inert Fraction Collector Internal Tray Assembly Analytical Scale 177 10 Parts for Maintenance Internal Tray Assembly 178 1260 Infinity Bio inert Fraction Collector This chapter provides information on cables used with the Agilent modules 1260 Infinity Bio inert Fraction Collector 11 Identifying Cables Cable Overview 180 Analog Cables 182 Remote Cables 184 BCD Cables 187 CAN LAN Cables 189 External Contact Cable 190 RS 232 Cable Kit 191 ee Agilent Technologies 179
55. add 1 aditional half tray 2 well plates 10 x 2 mL vials one possible additional half tray 100 x 2 mL vials one possible additional half tray Half trays up to 3 per fraction collector 15 x 6 mL vial 40 x 2 mL vial Plates for well plate trays 2 or 4 per fraction collector depending on well plate tray Eppendorf safe lock tubes 27 x 0 5 mL 1 5 mL or 2 5 mL 24 test tubes 18 mm OD 54 x 2 mL vial 15 x 6 mL vial Optional Time slices and peak threshold up downslope upper threshold and timetable Boolean logic for different detector signals combination of different modes manual trigger supported with Agilent 1200 Series Instant Pilot 1260 Infinity Bio inert Fraction Collector Site Requirements and Specifications 2 Table3 Specifications Agilent 1260 Infinity Analytical Bio inert Fraction Collector G5664A Type Specifications Trigger source Agilent 1200 Infinity Series VWD MWD and DAD detectors Agilent 6100 Series Quadrupole LC MS ELSD FLD RID third party detectors require UIB Diverter valve 3 2 valve switching time lt 100 ms Max pressure 6 bar diverter valve Environment 4 55 C constant temperature lt 95 humidity non condensing Materials in flow path PEEK Ceramic PTFE pH range 1 13 short term 14 For solvent compatibility refer to section Solvent information for parts of the 1260 Infinity Bio in ert LC system in the manual Only one type of
56. ails p n Description G1369B or Interface board LAN G1369 60002 G1369C or Interface board LAN G1369 60012 One board is required per Agilent 1260 Infinity instrument It is recommended to add the LAN board to the detector with highest data rate For the configuration of the G1369 LAN Communication Interface card refer to its documentation The following cards can be used with the Agilent 1260 Infinity modules Table 28 LAN Boards Type Vendor Supported networks Interface board LAN p n Agilent Technologies Fast Ethernet Ethernet 802 3 G1369B or G1369 60002 or RJ 45 10 100Base TX Interface board LAN p n recommended for re ordering G1369C or G1369 60012 LAN Communication Interface Agilent Technologies Fast Ethernet Ethernet 802 3 board p n G1369A or RJ 45 10 100Base TX G1369 60001 obsolete J4106A Hewlett Packard Ethernet 802 3 RJ 45 10Base T 1260 Infinity Bio inert Fraction Collector Hardware Information 12 Table 28 LAN Boards Type Vendor Supported networks J4105A Hewlett Packard Token Ring 802 5 DB9 RJ 45 10Base T J4100A Hewlett Packard Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX BNC 10Base2 1 These cards may be no longer orderable Minimum firmware of these Hewlett Packard JetDirect cards is A 05 05 Recommended LAN Cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair
57. ain Assemblies 164 Supported Trays for a Fraction Collector 166 List of Recommended Vials and Caps 167 Recommended Plates and Closing Mats 170 Fraction Collector Accessory Kit 172 Fraction Collector Thermostat 173 Needle Assembly 174 Diverter Valve Assembly 175 Tubing Kits 176 Internal Tray Assembly 177 1 Identifying Cables 179 Cable Overview 180 Analog Cables 182 Remote Cables 184 BCD Cables 187 CAN LAN Cables 189 External Contact Cable 190 RS 232 Cable Kit 191 12 Hardware Information 193 Electrical Connections 194 Interfaces 196 Setting the 8 bit Configuration Switch without On board LAN 202 Optional Interface Boards 206 13 Appendix 211 General Safety Information 212 The Waste Electrical and Electronic Equipment Directive 215 Lithium Batteries Information 216 Radio Interference 217 Sound Emission 218 Agilent Technologies on Internet 219 1260 Infinity Bio inert Fraction Collector Contents 8 1260 Infinity Bio inert Fraction Collector 1260 Infinity Bio inert Fraction Collector ee 1 66 Introduction e E e j Introduction to the Fraction Collector 10 Bio inert Materials 10 Early Maintenance Feedback 12 Instrument Layout 13 This chapter gives an introduction to the module Agg Agilent Technologies 1 Introduction to the Fraction Collector The G5664A is a fraction collector for analytical scale fractionation The bio inert fraction collector is designed with
58. ange the needle assembly e change the needle capillary carrier assembly e change the valve to needle tubing e change the diverter valve After the maintenance or repair task has been finished click End to move the transport assembly to the Home position 1260 Infinity Bio inert Fraction Collector Test Functions 8 Home Position This maintenance function moves the arm up and to the right rear for better access and exchange of the trays Park Arm This maintenance position moves the arm to the park position at the upper rear left side of the tray for transporting or shipping the fraction collector 1260 Infinity Bio inert Fraction Collector 119 8 Test Functions Transport Unit Self Alignment When CAUTION The transport unit alignment is required to compensate for larger deviations in positioning the needle carrier assembly This might be necessary after disassembling the system or when you exchange the transport unit the needle carrier assembly or the MTP main board This function is in the Diagnose screen of the ChemStation the Instant Pilot and Lab Advisor After disassembling the module When exchanging the transport unit the needle capillary carrier assembly the MTP main board Damage to the module If the Transport Unit Self Alignment is started with well plates on the tray the alignment procedure is aborted WITHOUT error message gt The sample transport self alignment requires the 4 well plate tray p n
59. bable cause Suggested actions 1 Disconnected or defective CAN cable Check the interconnection between the modules 2 Disconnected or defective 24V CAN DC OUT Reconnect the UIB Valve Start a test cable for an external valve analysis run 3 Defective UIB external Valve or MTP board Switch power off on complete system off then on Start a test analysis run e Please contact your Agilent service representative 114 1260 Infinity Bio inert Fraction Collector Error Information 7 Movement to Next Position Failed Error ID 4957 The transport mechanism detected an unexpected situation during the movement to the next fraction position Probable cause Suggested actions 1 Mismatch between tray configuration and Check the configuration and the loading of the the loading of the trays e g 4 well plates are tray configured in the UI but only three are loaded or shallow plates are configured but deep well plates are used or tube height doesn t match the configuration Could Not Find a Valid Next Position Error ID 4958 There has been more fractions than fraction positions Probable cause Suggested actions 1 Unexpected number of fractions e If possible use a tray with more positions Add an additional G5664 to the system 2 Start of an analysis sequence run Ensure that there are enough fraction positions without changing of the tray of the previous for the complete analysis sequence run run 12
60. before the initilization is finished Probable cause Suggested actions 1 The side door is not installed Install the side door 2 The door is bent or the magnet is Change the side door misplaced broken 3 The sensor on the MTP board is defective Please contact your Agilent service representative 4 Excessive weight on top of the fraction Check stack configuration and reduce weight on collector see also Initialization Failed on top of the fraction collector page 112 Arm Movement Failed or Arm Movement Timeout Error ID 4002 The transport assembly was unable to complete a movement in one of the axes The processor defines a certain time window for the successful completion of a movement in any particular axis The movement and position of the transport assembly is monitored by the encoders on the stepper motors If the processor does not receive the correct position information from the encoders within the time window the error message is generated Axes identification e Arm Movement 0 Failed X axis e Arm Movement 1 Failed Z axis e Arm Movement 2 Failed Theta needle carrier rotation 1260 Infinity Bio inert Fraction Collector 107 7 108 Error Information Probable cause 1 Mechanical obstruction 2 High friction in the transport assembly 3 Defective motor assembly 4 Defective sample transport assembly flex board 5 Defective main board Suggested actions Ensure unobstructed movement
61. ble 17 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Pumps G1310B Iso Pump 2 Yes No Yes 1 Yes G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump G4220A B Bin Pump 2 No Yes Yes No Yes G1361A Prep Pump 2 Yes No Yes No Yes CAN DC OUT for CAN slaves Samplers G1329B ALS 2 Yes No Yes No Yes THERMOSTAT for G2260A Prep ALS G1330B G1364B FC PS 2 Yes No Yes No Yes THERMOSTAT for G1364C FC AS G1330B G1364D FC uS CAN DC OUT for CAN G1367E HiP ALS slaves G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler G4226A ALS 2 Yes No Yes No Yes 196 1260 Infinity Bio inert Fraction Collector Hardware Information 12 Table 17 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes G4212A B DAD 2 No Yes Yes 1 Yes G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 1 Yes G1362A RID G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or with additional G1369C LAN
62. can be replaced funnel screw 1 seals 2 tubing 3 or funnel holder 4 1 2 3 4 5 Turn the internal tray upside down Slide the funnel s 6 Insert the funnel into the tray and screw it tight clockwise 155 9 Maintenance Repairing or Exchanging a Funnel of the Internal Tray 7 Re install the plug that holds the flow delay sensor 1 8 Re install the internal tray and make sure to route the pull the waste tubing tight through the flow delay sensor waste tubing out through the outlet of the instrument 2 and mount the waste tubing 3 correctly see Exchanging the Internal Tray on Next Steps 9 Re install the tray s in the tray base 10 Start the instrument 11 Close the front cover 156 1260 Infinity Bio inert Fraction Collector Maintenance 9 Exchanging the Leak Sensor Exchanging the Leak Sensor When If defective Parts required p n Description 5061 3356 Leak sensor Preparations e Position the transport unit of the fraction collector in the Home position see Maintenance Functions on page 118 Remove all installed trays from the tray base Turn OFF the instrument 1 Unscrew the front tray guide 3 screws 2 Unplug the leak sensor from the SLF board 3 Screws 1260 Infinity Bio inert Fraction Collector 157 9 Maintenance Exchanging the Leak Sensor 3 Notice the position of the leak sensor and pull out the old
63. can give some contamination in the system The adhesive is soluble in most of the solvents used in HPLC gt In general do not use closing mats with adhesive The fraction collector has no prepunch needle therefore the adhesive will clog the needle after several injections 1 Press the front door latch holding button located at the front of the right side cover 2 Lift the front door 3 Adjust the top plate of the test tube tray for the correct tube height if required 4 Load the fraction collector tray with fraction collector well plates test tubes or vials as required 5 Slide the fraction collector tray into the fraction collector so that the rear of the tray is seated firmly against the rear of the tray area Installed trays are automatically detected and identified 1260 Infinity Bio inert Fraction Collector 43 3 44 Installing the Fraction Collector 6 Press the front of the fraction collector tray down to secure the tray in the fraction collector 7 Close the front door If the tray pops out of position the air channel adapter is not correctly inserted Before starting a run the instrument has to be correctly configured in the user interface Numbering of Vial Test Tube and Well plate Positions With the 4 plates full tray e Plate in the left front position P1 e Plate in the left back position P2 Plate in the right front position P3 e Plate in the right back position P4 e Vessel Al A2 B
64. contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that
65. cs based on Al Og are inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Data above were collected from external resources and are meant as a reference Agilent cannot guarantee the completeness and correctness of such information Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually increases at higher temperatures If in doubt consult additional resources 1260 Infinity Bio inert Fraction Collector 87 4 Using the Fraction Collector Solvent Information 88 1260 Infinity Bio inert Fraction Collector 1260 Infinity Bio inert Fraction Collector e e e e0 0e 7 e ed to Optimize the Fraction Collector a e ss Optimizing Fraction Collection 90 This chapter provides information on how to optimize the module Agilent Technologies 5 How to Optimize the Fraction Collector Optimizing Fraction Collection 90 Time based fraction collection Time slices must have a length of at least 0 05 min Set of Fractions such that length of resulting fractions is at least 0 05 min Peak based fraction collection Set threshold and slope values such that length of fractions is at least 0 05 min Unresolved peaks can be separated using appropriate threshol
66. ctive earthing and has been manufactured and tested according to international safety standards Operation 212 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired 1260 Infinity Bio inert Fraction Collector Appendix 13 the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not att
67. d and slope values If two unresolved peaks are to be collected as one fraction collect based on threshold only If the baseline of the chromatogram is below or above 0 mAU this offset is not accounted for when triggering peaks using a threshold value The threshold value is always added to 0 mAU 1260 Infinity Bio inert Fraction Collector 1260 Infinity Bio inert Fraction Collector 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 92 Status Indicators 93 Power Supply Indicator 93 Module Status Indicator 94 Agilent Lab Advisor Software 95 Overview about the troubleshooting and diagnostic features ee Agilent Technologies 91 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 92 Status Indicators The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Maintenance Functions The maintenance functions position the transport unit and needle carr
68. detected during the delay calibration Probable cause Suggested actions 1 Wrong sample has been used for the delay Check method and delay calibration procedure calibration 2 Wrong method has been used for the delay Check method and delay calibration procedure calibration 3 Air bubbles are in the flow path Check flow path for leaks and air bubbles Valve Switch Failed Error ID 4959 If multiple fraction collectors are configured an external valve G1160A is used to switch between the fraction collectors The error message is displayed if the external valve failed to switch to next position Probable cause Suggested actions 1 Valve is blocked Possible if eluents with Purge valve to dissolve crystals highly concentrated electrolytes are used 2 Power cord for the valve is not connected Check power cord connection 3 Valve drive or valve electronics are defective Synchronize the valve Exchange the valve 110 1260 Infinity Bio inert Fraction Collector Adapter Required Error ID 4961 Error Information 7 The wellplate adapter is required for the following operation Probable cause 1 Wellplate adapter not attached 2 Cable between needle carrier assembly to transport unit assembly disconnected or defective 3 Needle carrier assembly defective Funnel Not Supported Error ID 4962 Suggested actions Attach wellplate adapter Check cable between needle carrier assembly to transport unit ass
69. dorf tubes 1 pk Closing mat for all 96 Agilent plates Only one type of well plates can be used at a time in one tray 1260 Infinity Bio inert Fraction Collector 171 10 Parts for Maintenance Fraction Collector Accessory Kit p n Description G1364 68755 Accessory Kit p n Description 0100 1711 1 16 x 10 32 Male Nut PEEK 0100 1856 3 16 ID Tube Barb Y Ftg PP OBS 5042 6421 Micro Clamp 5042 6472 Clamp Waste Tube 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 5181 1519 CAN cable Agilent module to module 1 m 8710 2476 HEX ALLEN KEY 2 0 MM A F G1313 44512 Halftray for 40 x 2 mL vials G1313 44513 Halftray for 15 x 6 mL vials G1329 43200 Adapter air channel 172 1260 Infinity Bio inert Fraction Collector Parts for Maintenance 10 Fraction Collector Thermostat Fraction Collector Thermostat Figure 31 Fraction Collector Thermostat Item p n Description 1 G1330 69030 FRC thermostat exchange assembly 1260 Infinity Bio inert Fraction Collector 173 10 Parts for Maintenance Needle Assembly p n Description G5667 87200 Needle assembly bio inert yf For bio inert modules use bio inert parts only 174 1260 Infinity Bio inert Fraction Collector Parts for Maintenance 10 Diverter Valve ibly Diverter Valve Assembly Item p n Description 1 G5664 60901 3 2 Way valve assembly bio inert 2 0515 1211 PIN screw yy For bio inert modules use bio inert parts only Figure 32
70. e connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL E 9 1 White Digital ground NC 2 Brown Prepare run Low 80 7 3 3 Gray Start Low x NC 4 Blue Shut down Low oO e o gt NC 5 Pink Not connected le NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators p n 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1260 Infinity Bio inert Fraction Collector Identifying Cables 11 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low NC 4 Blue Shut down Low e o NC 5 Pink Not connected le NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 A D Pin Agilent Signal Name Active module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue S
71. ed to collect a fixed number of equal fractions in a give time interval This time interval is defined by the time value in the current and following timetable line e Timeslices min can be edited to collect fractions with a defined collection time With this option the collection time of the last fraction can be shorter This depends on the overall runtime For editing the Timetable the functions Insert Append Cut Copy and Paste are offered To access the additional sections in the Setup Fraction Collector dialog box click More Time In the time section of the dialog box the Stoptime and the Posttime for the fraction collector can be specified By default the Stoptime is set to as pump and the posttime is switched OFF 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Rinse Fraction Collection Needle Analytical Scale only If Discrete fractions is selected as Collection Mode see also Collection Mode on page 54 you can setup a needle rinse step before the fraction collection and or between fractions Then the needle will move to the funnel on the internal tray and the diverter valve will switch to flush the needle in order to avoid carry over from the previous fraction The instrument will determine if it is possible to rinse the needle before the next fraction is expected If you have recovery positions in your fraction collector or if you are using one fraction collector for sample recovery in
72. eedle Tubing 136 Exchanging the Needle Assembly 140 Exchanging the Needle Capillary Carrier Assembly 143 Exchanging the Diverter Valve 148 Exchanging the Internal Tray 152 Repairing or Exchanging a Funnel of the Internal Tray 154 Exchanging the Leak Sensor 157 Exchanging or Installing the Optional Interface Board 160 Replacing Module Firmware 162 This chapter describes the maintenance of the module ott Agilent Technologies 125 9 Maintenance Introduction to Maintenance The module is designed for easy maintenance Maintenance can be done from the front with module in place in the system stack There are no serviceable parts inside Do not open the module 126 1260 Infinity Bio inert Fraction Collector Maintenance 9 Cautions and Warnings Module is partially energized when switched off as long as the power cord is plugged in Risk of stroke and other personal injury Repair work at the module can lead to personal injuries e g shock hazard when the module cover is opened and the instrument is connected to power gt Never perform any adjustment maintenance or repair of the module with the top cover removed and with the power cord plugged in gt The security lever at the power input socket prevents that the module cover is taken off when line power is still connected Never plug the power line back in when cover is removed Sharp metal edges Sharp edged parts of the equipment may cause injuries
73. een status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e a red LED may indicate a problem of a different module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of main firmware A fast blinking indicator indicates that the module is in a low level error mode In such a case try to re boot the module or try a cold start see Special Settings on page 205 Then try a firmware update see Replacing Module Firmware on page 162 If this does not help a main board replacement is required 1260 Infinity Bio inert Fraction Collector Troubleshooting and Diagnostics 6 Agilent Lab Advisor Software The Agilent Lab Advisor sof
74. embly Exchange needle carrier assembly Funnels are only supported for the analytical scale fraction collector Consequently the error message is displayed if a tray with funnels configured for the preparative or the micro collector spotter Probable cause 1 Fraction collector type and tray not compatible 1260 Infinity Bio inert Fraction Collector Suggested actions Check your tray configuration Select Instrument Agilent 1100 1200 System gt More Agilent 1100 1200 Fraction Collector gt Configuration Funnel trays are only supported for the analytical scale fraction collector Check your 1260 Infinity System Configuration Select Instrument Agilent 1100 1200 System gt More Agilent 1100 1200 Fraction Collector gt Configure 1100 1200 Access and verify that an analytical scale fraction collector is configured 111 7 112 Error Information Pusher Wrong or Defect Error ID 4965 Probable cause Suggested actions 1 Wellplate adapter not attached Attach wellplate adapter 2 Needle carrier assembly defective Exchange needle carrier assembly Wrong or Missing Needle Analytical Scale Error ID 4966 Probable cause Suggested actions 1 No needle installed Check which needle has been installed 2 Short needle for high flow rates installed but Remove wellplate adapter if short needle for the wellplate adapter hasn t been removed semi preparative operation is installed Initialization Fai
75. empt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1260 Infinity Bio inert Fraction Collector 213 13 Appendix Safety Symbols Table 29 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the A instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced G
76. er operating conditions the radio interference limits are still met within the premises 1260 Infinity Bio inert Fraction Collector 217 13 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 218 1260 Infinity Bio inert Fraction Collector Appendix 13 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the modules for download 1260 Infinity Bio inert Fraction Collector 219 Index Index 8 bit configuration switch without On Board LAN 202 A adapter required 111 Agilent Diagnostic software 95 Agilent Lab Advisor software 95 Agilent Lab Advisor 95 Agilent oninternet 219 ambient non operating temperature 19 ambient operating temperature 19 analog signal 200 analog cable 182 apgremote 200 battery safety information 216 BCD board external contacts 206 BCD cable 187 bench space 18 bio inert 128 materials 10 board HP JetDirect card 208 boards LAN card 208 220 C cable analo
77. er describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 1260 Infinity Bio inert Fraction Collector In This Guide 8 Test Functions This chapter describes the module s built in test functions 9 Maintenance This chapter describes the maintenance of the module 10 Parts for Maintenance This chapter provides information on parts for maintenance 11 Identifying Cables This chapter provides information on cables used with the Agilent modules 12 Hardware Information This chapter describes the module in more detail on hardware and electronics 13 Appendix This chapter provides addition information on safety legal and web 4 1260 Infinity Bio inert Fraction Collector Contents Contents Introduction 9 Introduction to the Fraction Collector 10 Bio inert Materials 10 Early Maintenance Feedback 12 Instrument Layout 13 Site Requirements and Specifications 15 Site Requirements 16 Physical Specifications 19 Performance Specifications 20 Installing the Fraction Collector 23 Unpacking the module 24 Optimizing the Stack Configuration 26 Installing the Module 30 Installing a Thermostatted Fraction Collector 35 Flow Connections to the Fraction Collector 40 Fraction Collector Trays 43 Configure Well plate Types 46 Transporting the Fraction Collector 50 Using the Fraction Collector 51 Configuration of the Fraction
78. er tight fittings of the inlet waste tubing assembly to the ports of the diverter valve View from the bottom NOTE The tubings must not be bent up or downwards The cables must not be twisted 7 A color coded ring on one of the tubings and the valve body indicates which cable belongs to which port 134 1260 Infinity Bio inert Fraction Collector Maintenance 9 Replacing the Inlet Waste Tubings NOTE It is absolutely vital to connect these tubings as described in order to maximize their lifetime and operating security Next Steps 9 Re install the tray s in the tray base 10 Start the instrument 11 Close the front cover IMPORTANT The cables must run into the ports of the diverter valve in lines parallel to the horizon 1260 Infinity Bio inert Fraction Collector 135 9 Maintenance Replacing the Valve to Needle Tubing When When contaminated worn out or visibly damaged Typically once every year Tools required p n Description 8710 1534 Wrench open end 4 mm 8710 0510 Wrench open 1 4 5 16 inch Parts required p n Description G5664 68712 Analytical tubing kit 0 25 mm i d PTFE ESD Preparations Position the transport unit of the fraction collector in the Home position see Maintenance Functions on page 118 Remove all installed trays from the tray base Position the
79. erials see Bio inert Materials on page 10 in the flow path also referred to as wetted parts which are widely accepted by life scientists as they are known for optimum inertness to biological samples and ensure best compatibility to common samples and solvents over a wide pH range Explicitly the complete flow path is free from stainless steel and free from other alloys containing metals such as iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction contains no metals whatsoever However there are no materials that combine suitability for versatile HPLC instrumentation valves capillaries springs pump heads flow cells etc with complete compatibility with all possible chemicals and application conditions This section recommends the preferred solvents Chemicals that are known to cause issues should be avoided or exposure should be minimized for example for short term cleaning procedures After potentially aggressive chemicals have been used the system should be flushed with compatible standard HPLC solvents 1260 Infinity Bio inert Fraction Collector 85 4 86 Using the Fraction Collector PEEK PEEK Polyether Ether Ketones combines excellent properties with regard to biocompatibility chemical resistance mechanical and thermal stability and is therefore the material of choice for biochemical instrumentation It is stable in t
80. esponse jo g fi Pages be Help 76 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Special Applications Pooling CAUTION We define pooling as collecting fractions from multiple injections into the same fraction locations In the Agilent ChemStation there are two ways to initiate pooling e Ifyou want to collect multiple injections from the one sample vial or sample well into the same fraction location specify multiple injections in the Sequence Table Incase you want to collect multiple injections from the several sample vials or sample wells into the same fraction location specify the same Fract Start for multiple samples in the Sequence Table An example is displayed in Figure 23 on page 77 In this sequence the sample 1 will be injected from plate 1 position Al of the Agilent 1260 Infinity High Performance Autosampler and fraction collection will start at plate 1 position Al of the fraction collector For the next sample 2 which is injected from a different location in the high performance autosampler the fraction collection will start again at plate 1 position A1 of the fraction collector Line Location Sample Name Dilution Datafile Inj Volume Frac Start LimsID Target Masses 1 P1401 Sample 1 2 P1402 Sample 2 1 P1 4 01 3 P1403 Sample 3 1 P1 4 01 l l l l l l Figure 23 Pooling sequence Data loss due to pump shut down If yo
81. g 182 BCD 187 CAN 189 external contact 190 LAN 189 remote 184 RS 232 191 cables analog 180 BCD 180 CAN 181 external contact 181 LAN 181 overview 180 remote 180 RS 232 181 calib delay vol two peaks 110 CAN cable 189 cleaning 129 cluster partner lost during analysis 114 Communication settings RS 232C 203 compensation sensor open 103 compensation sensor short 104 condensation 18 could not find a valid next position 115 D defect on arrival 24 Diagnostic software 95 dimensions 19 E electrical connections descriptions of 194 electronic waste 215 electrostatic discharge ESD EMF early maintenance feedback 12 128 160 error messages calib delay vol two peaks 110 cluster partner lost during analysis 114 could not find a valid next position 115 lost CAN partner 102 motor temperature 113 remote timeout 101 vessel stuck to needle 114 error messages adapter required 111 arm movement 107 compensation sensor open 103 compensation sensor short 104 fan failed 104 ignition without cover leak sensor open 103 leak sensor short 102 leak 105 shutdown 101 timeout 100 external contact cable 190 external contacts BCD board 206 105 105 1260 Infinity Bio inert Fraction Collector F fan failed 104 firmware updates 162 162 upgade downgrade 162 upgrade downgrade 162 frequency range 19 G general error messages 100 HP JetDirect card 208 humidity 19 installation bench space 18
82. ges 100 Timeout 100 Shutdown 101 Remote Timeout 101 Lost CAN Partner 102 Leak Sensor Short 102 Leak Sensor Open 103 Compensation Sensor Open 103 Compensation Sensor Short 104 Fan Failed 104 Leak 105 Open Cover 105 Module Error Messages 106 Exhaust Fan Failed 106 Front Door Error 106 Side Door Error 107 Arm Movement Failed or Arm Movement Timeout 107 Needle to Needle Rinse Funnel Position Failed 108 Needle Carrier Failed 109 Missing Vial or Missing Well plate 109 Calib delay vol two peaks 110 Valve Switch Failed 110 Adapter Required 111 Funnel Not Supported 111 Pusher Wrong or Defect 112 Wrong or Missing Needle Analytical Scale 112 Initialization Failed 112 ot Agilent Technologies 97 7 Error Information Motor Temperature 113 Vessel Stuck to Needle 114 Cluster Partner Lost During Analysis 114 Movement to Next Position Failed 115 Could Not Find a Valid Next Position 115 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 98 1260 Infinity Bio inert Fraction Collector Error Information 7 What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator
83. gilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module n 1 1 BCD 5 20 ae 2 2 BCD 7 80 K 3 3 BCD 6 40 4 4 BCD 4 10 g 5 5 BCDO 1 6 6 BCD 3 8 z 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 188 1260 Infinity Bio inert Fraction Collector CAN LAN Cables Identifying Cables 11 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection 1260 Infinity Bio inert Fraction Collector 189 11 Identifying Cables External Contact Cable 190 5 10 L115 6 O B 11 One end of this cable provides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent Module Interface Board to general purposes p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 a Brown 2 EXT 1 x Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green
84. he specified pH range and inert to many common solvents There is still a number of known incompatibilities with chemicals such as chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc When used above room temperature PEEK is sensitive to bases and various organic solvents which can cause it to swell As normal PEEK capillaries are very sensitive to high pressure especially under such conditions Agilent uses stainless steel cladded PEEK capillaries to keep the flow path free of steel and to ensure pressure stability to at least 600 bar If in doubt consult the available literature about the chemical compatibility of PEEK Titanium Titanium is highly resistant to oxidizing acids for example nitric perchloric and hypochlorous acid over a wide range of concentrations and temperatures This is due to a thin oxide layer on the surface which is stabilized by oxidizing compounds Reducing acids for example hydrochloric sulfuric and phosphoric acid can cause slight corrosion which increases with acid concentration and temperature For example the corrosion rate with 3 HCl about pH 0 1 at room temperature is about 13 um year At room temperature titanium is resistant to concentrations of about 5 sulfuric acid about pH 0 3 The addition of nitric acid
85. hut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 1260 Infinity Bio inert Fraction Collector 185 11 186 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL White 1 Digital ground A Q 1 ou Brown 2 Prepare run Low 00 pees DE KEY Gray 3 Start Low oo poe 00 S EE te 4 Shut down Low og Pink 5 Not connected oo 26 Yellow 6 Power on High 5 15 Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 1260 Infinity Bio inert Fraction Collector Identifying Cables 11 BCD Cables One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 Violet 2 BCD7 80 a Blue 3 BCD 6 40 m Yellow 4 BCD 4 10 SS Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low 1260 Infinity Bio inert Fraction Collector 187 11 Identifying Cables A
86. ier assembly on certain positions for maintenance homing parking or delay calibration see Maintenance Functions on page 118 Transport Unit Self Alignment The transport unit alignment with the transport unit and the well plate tray is required to compensate for larger deviations in positioning the needle carrier assembly The transport unit self alignment is required after disassembling the system or when you exchange the transport unit the needle carrier assembly or the MTP main board This function is in the Instant Pilot and Lab Advisor 1260 Infinity Bio inert Fraction Collector Troubleshooting and Diagnostics 6 Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status Status indicator green yellow red Line power switch green light Figure 26 Location of status indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 1260 Infinity Bio inert Fraction Collector 93 6 Troubleshooting and Diagnostics Module Status Indicator The module status indicator indicates one of six possible module conditions 94 When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis A gr
87. ignal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces Some modules have module specific interfaces connectors They are described in the module documentation 1260 Infinity Bio inert Fraction Collector 201 12 Hardware Information Setting the 8 bit Configuration Switch without On board LAN 202 The 8 bit configuration switch is located at the rear of the module This module does not have its own on board LAN interface It can be controlled through the LAN interface of another module and a CAN connectio
88. injections When using the standard tray for two well plates and 10 funnels only ten injections per sequence are executed 1260 Infinity Bio inert Fraction Collector 79 4 Using the Fraction Collector Limitations and How to Avoid Problems 80 Rinse Fraction Collection Needle If Rinse Fraction Collection Needle is set to Between fraction collection at least 0 3 min are required to perform this task When doing time based fraction collection rinsing the needle is only possible between two time table entries which must have a gap of at least 0 3 min For peak based fraction collection a time gap of also at least 0 3 min is required If a new peak is detected during the rinse process it is aborted and the needle moves back to the next free fraction position Depending on flow rate and delay volume VD1 the beginning of this peak may be lost If you have recovery positions in your fraction collector or if you are using one fraction collector for sample recovery in a multiple fraction collector configuration the rinse function between fraction collection is ignored Needle Movement The option into location under Needle Movement in the fraction collector configuration must only be used for capped 2 or 5 mL vials or well plates Using other or open vials with this command can lead to a Movement failed error Replacing fraction containers When replacing filled tubes vials or well plates from the fraction collector make sure to re
89. inlets caps and septa you have to specify the x size of the septa Size of the well in y direction Plate width If you use well plates with inlets caps and septa you have to specify the y size of the septa For round wells the relative of the top and bottom of the well 85 50 0 25 mm SBS Standard up to 47 mm up to 45 mm min 3 7 mm min 3 0 mm with position accuracy alignment micro scale down to 3 7 mm min 3 0 mm with position accuracy alignment micro scale gt 1 0 cylindrical well 0 0 conical well 48 1260 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 Table6 Configuring Well plate Types Location Description Definition Limits Square Click in the field to specify whether the Yes rectangular well is rectangular or round No round oval Is well plate Click in this field to specify if this is a Yes well plate or MALDI well plate or not Relevant for Target continuous flow operation No Vial Tray or Eppendorf tray The distances need to be measured with high precision It is recommended to use a caliper If possible contact the vendor of non predefined well plates to obtain these dimensions 1260 Infinity Bio inert Fraction Collector 49 3 Installing the Fraction Collector Transporting the Fraction Collector 50 When moving the fraction collector inside the laboratory no special precautions are needed However if the fraction collector needs
90. instrument layout 13 interfaces 196 internet 219 L LAN cable 189 communication interface board 208 eak sensor open 103 eak sensor short 102 eak 105 ine frequency 19 ine voltage 19 ithium batteries 216 ost CAN partner 102 M maintenance definition of 126 feedback 12 replacing firmware 162 162 materials bio inert 10 message ignition without cover 105 105 remote timeout 101 non operating altitude 19 non operating temperature 19 0 operating Altitude 19 operating temperature 19 P packaging damaged 24 physical specifications 19 power consumption 19 power supply indicator 93 power consideration 16 power cords 17 R radio interference 217 remote cable 184 repairs replacing firmware 162 162 RS 232C cable 191 communication settings 203 S safety class 212 safety information 1260 Infinity Bio inert Fraction Collector Index lithium batteries 216 safety general information 212 standards 19 symbols 214 shutdown 101 site requirements power cords 17 solvent information 85 sound emission 218 special interfaces 201 special settings boot resident 205 forced cold start 205 specification physical 19 status indicator 94 T temperature sensor 105 timeout 100 troubleshooting error messages 99 92 status indicators 92 93 V voltage range 19 wW waste electrical and electronic equipment 215 WEEE directive 215 weight 19 221 www agilent com In This Book This manua
91. ion 5181 1210 Crimp Cap silver aluminum septum clear PTFE red rubber 100 Pack 5183 4498 Crimp Cap silver aluminum septum clear PTFE red rubber 1000 Pack 5181 1215 Crimp Cap blue aluminum septum clear PTFE red rubber 100 Pack 1260 Infinity Bio inert Fraction Collector Parts for Maintenance 10 p n Description 5181 1216 Crimp Cap green aluminum septum clear PTFE red rubber 100 Pack 5181 1217 Crimp Cap red aluminum septum clear PTFE red rubber 100 Pack Snap Caps Caps for Use with the Analytical Scale Fraction Collector only p n Description 5182 0550 Snap Cap clear polypropylene septum clear PTFE red rubber 100 Pack 5182 3458 Snap Cap blue polypropylene septum clear PTFE red rubber 100 Pack 5182 3457 Snap Cap green polypropylene septum clear PTFE red rubber 100 Pack 5182 3459 Snap Cap red polypropylene septum clear PTFE red rubber 100 Pack Screw Caps Caps for Use with the Analytical Scale Fraction Collector only p n Description 5182 0717 Screw Cap blue polypropylene septum clear PTFE red rubber 100 Pack 5182 0718 Screw Cap green polypropylene septum clear PTFE red rubber 100 Pack 5182 0719 Screw Cap red polypropylene septum clear PTFE red rubber 100 Pack 5182 0720 Screw Cap blue polypropylene septum clear PTFE silicone 100 Pack 5182 0721 Screw Cap green polypropylene septum clear PTFE silicone 100 Pack 5182 0722 Screw Cap red polypropylene septum clear PTFE silico
92. ion Collector CAUTION Loss of data If the internal signal delay is longer than the delay time tp some of the peak will be lost gt The maximum allowed signal delay time can be calculated using the following equation Voy may v Flowrate Signal delay time gt After calculating the maximum signal delay time a Peakwidth setting can be selected that gives a signal delay time which is shorter than the calculated maximum signal delay time This Peakwidth setting should then be used for the LC purification run 62 We recommend to set the Peakwidth always to gt 0 01 for the DAD and MWD or to gt 0 005 for the VWD If the Peakwidth setting cannot be reduced and the signal delay time is longer than tp it is also possible to increase Vp by adding additional tubing However this higher delay volume will increase the peak dispersion between detector and fraction collector The stop time of the run in the ChemStation must be set to at least Total duration of time table time of last entry OFF fraction collector delay time Vp v 0 1 min for time based fraction collection End of last peak tg fraction collector delay time Vpj v 0 1 min for peak based fraction collection Performing a Delay Calibration with an UV Detector 1 Place a vial containing the Delay sensor calibrant p n G1946 85020 in position 1 of the autosampler 2 Perform Delay Calibration by using Lab Advisor B 02 01 or higher Follow
93. ion Collector Main Assemblies Fraction Collector Main Assemblies Figure 30 165 1260 Infinity Bio inert Fraction Collector 10 Parts for Maintenance Supported Trays for a Fraction Collector 166 For more information on trays well plates and collecting tubes for an LC Fraction Collector refer to the Agilent Web site http www chem agilent com en US Products columns supplies instrument parts lc pages gp10885 aspx http www chem agilent com en US Products columns supplies generalchro matography pages gp10886 aspx 1260 Infinity Bio inert Fraction Collector Parts for Maintenance 10 List of Recommended Vials and Caps Crimp Top Vials Caps for Use with the Analytical Scale Fraction Collector only p n Description 5181 3375 Crimp Top Vial 2 mL clear glass 100 Pack 5183 4491 Crimp Top Vial 2 mL clear glass 1000 Pack 5182 0543 Crimp Top Vial 2 mL clear glass write on spot 100 Pack 5183 4492 Crimp Top Vial 2 mL clear glass write on spot 1000 Pack 5183 4494 Crimp Top Vial 2 mL clear glass write on spot 100 Pack silanized 5182 3376 Crimp Top Vial 2 mL amber glass write on spot 100 Pack 5183 4493 Crimp Top Vial 2 mL amber glass write on spot 1000 Pack 5183 4495 Crimp Top Vial 2 mL amber glass write on spot 100 Pack silanized SnapTop Vials Caps for Use with the Analytical Scale Fraction Collector only p n 5182 0544 5183 4504 5183 4507 5182 0546 5183 4505 5183 4508 Descrip
94. ion inside the module can lead to overheating gt Place the fraction collector thermostat with 25 cm 10 inch space on each side for sufficient air circulation 36 1 Place the thermostat on the bench 2 Remove the front cover and route the condensation drain tube to a waste container 3 Install the LAN interface board in the fraction collector if required see Exchanging or Installing the Optional Interface Board on page 160 Remove the adhesive tape which covers the side and front doors Open the front door and remove the left side door Remove the transport protection foam y oO Oo A Install the corrugated waste tube in the plastic port at the front bottom center of the fraction collector and route down into a waste container Slide the waste tubing coming from the internal tray if present through the plastic port and the corrugated waste tube see Figure 3 on page 32 Route the corrugated waste tubing into a waste container 8 Re install the left side door take care of the magnet at the back Ensure the side door is correctly installed its presence is sensed by a hall sensor a missing side door will result in a NOT READY state of the instrument 9 Place the fraction collector on top of the thermostat Make sure that the fraction collector is correctly engaged in the thermostat locks 1260 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 Installing a Thermostatted Fraction Co
95. l contains technical reference information about the Agilent 1260 Infinity Bio inert Analytical scale Fraction Collector G5664A The manual describes the following e introduction to the fraction collector e site requirements and specifications installing the fraction collector e using and optimizing e troubleshooting and test functions maintenance e parts and materials e hardware cables and e safety information Agilent Technologies 2011 2012 Printed in Germany 05 2012 G5664 90001 Rev B fe Agilent Technologies
96. led Error ID 4950 The fraction collector failed to complete initialization correctly The fraction collector initialization procedure moves the needle arm and transport assembly to their home positions in a predefined routine During initialization the processor monitors the position sensors and motor encoders to check for correct movement If one or more of the movements is not successful or is not detected the error message is generated Probable cause Suggested actions 1 Transport unit not aligned correctly Perform an auto alignment 2 Mechanical obstruction Ensure unobstructed movement of the transport assembly 3 Defective transport assembly flex Please contact your Agilent service representative board 1260 Infinity Bio inert Fraction Collector Error Information 7 Probable cause Suggested actions 4 Defective MTP main board Please contact your Agilent service representative 5 Excessive weight on top of the fraction Check stack configuration and reduce weight on top collector see also Side Door Error on of the fraction collector page 107 Motor Temperature Error ID 4027 4040 4261 4451 One of the motors of the transport assembly has drawn excessive current causing the motor to become too hot The processor has switched off the motor to prevent damage to the motor Motor identification e Motor 0 temperature X axis motor e Motor 1 temperature Z axis motor e Motor 2 temperature Theta motor
97. llector 10 Remove the plastic cover from the tray base place the air channel adapter 1 into the fraction collector tray base Make sure the adapter is fully pressed down This assures that the cold airstream from the thermostat is correctly guided to the tray area of the fraction collector Place the plug channel 2 on top of the air channel adapter Both devices must be installed correctly to assure proper operation of the instrument Y v a Figure 6 Installation of thermostat and fraction collector 11 The fraction collector is delivered with a pre installed tray compartment divider This divider should only be used with the thermostatted fraction collector if a half or std tray is installed into the left and center positions of the instrument If any tray is installed to the right side of the instrument remove the tray compartment divider The tray compartment divider optimizes the cooling performance of the instrument if only the left and center position of the fraction collector are in use Figure 7 Removing the Tray Compartment Divider 1260 Infinity Bio inert Fraction Collector 37 3 38 Installing the Fraction Collector 12 Install the tray you have ordered for your fraction collector The test tube trays can be adjusted in height depending on the height of the used test tubes To adjust the height of the trays press against the snappers at the guides in the four corners of a tray and move the top plate of the t
98. llector 4 The Delay Calibration can only be performed for fraction collector 1 of your configured system The calculated delay volume is used for all fraction collectors therefore the volume tubing between the selection valve and each installed fraction collector must be identical Otherwise fraction collector 2 and fraction collector 3 have to be temporarily configured as fraction collector 1 during the delay calibration The fraction collector inlet tubings have to be connected to port 1 3 of the selection valve in the same order as configured in the ChemStation The recovery fraction collector is always connected to the waste tubing of the main fraction collectors The waste tubing from multiple fraction collectors and the inlet tubing to the recovery collector have to be connected through a T piece e Fraction collector 4 will always be used as a recovery fraction collector 1260 Infinity Bio inert Fraction Collector 57 4 Using the Fraction Collector Delay Volumes and Delay Calibration Figure on page 58 shows a schematic drawing of the flow path between the detector and the fraction collector with the two delay volumes Vp and Vp For peak based fraction collection the system delay times tp and tpg can be calculated by dividing the delay volumes by the flow rate Diverter valve Waste The delay volume Vp is a system parameter it depends on the installed fraction collector tubing and the fraction collector
99. low the described installation procedures to maximize the lifetime of inlet waste tubing assembly and the valve to needle tubing and to avoid potential spills or fraction losses gt Regularly inspect the tubings and exchange them if they are worn out or show visible signs of damage 132 1260 Infinity Bio inert Fraction Collector Maintenance 9 Replacing the Inlet Waste Tubings 1 Locate the diverter valve with the finger tight fittings of 2 Unscrew the two finger tight fittings of the inlet waste the inlet waste tubing assembly the figure shows the tubing assembly at the diverter valve open fraction collector seen from the front 3 Unplug the inlet waste tubing assembly from the bolt 4 Install the new inlet waste tubing assembly into the bolt carrier located on the top center of the front cover by carrier as shown below Slide in the long ends of the moving the snapper as indicated by the arrows Slide the tubings from bottom to top and let the snapper click into tubings out to the bottom position 1260 Infinity Bio inert Fraction Collector 133 9 Maintenance Replacing the Inlet Waste Tubings 5 The rear tubing with the label with the arrow must be connected to the flow cell of the detector The front tubing must be inserted into the waste container Yo a X N From flow cell 6 Connect the fing
100. lve to needle tubing from the needle Viewed from the bottom 4 Un clip the tubing from the bottom of the needle carrier assembly 1260 Infinity Bio inert Fraction Collector 137 Maintenance Replacing the Valve to Needle Tubing Slide the tubing through the hole in the needle carrier assembly from bottom to top and out of the holder in the z arm assembly Install the new valve to needle tubing assembly by clipping it in to the holder in the z arm assembly 1 Important and slide it through the hole in the z arm 2 and out on the bottom of the needle carrier assembly top to bottom Screw the finger tight fitting into the port of the diverter valve 3 7 Using the 4 mm wrench and the 5 16 wrench for counter holding connect the valve to needle tubing to the needle Viewed from the bottom IMPORTANT After fixing the screw clip the tubing into the guide on the bottom of the needle carrier assembly It is absolutely vital that the tubing is installed as described to maximize the lifetime of the tubing 138 1260 Infinity Bio inert Fraction Collector Maintenance 9 Next Steps 9 Re install the needle to the needle carrier assembly if you previously removed it Make sure to slide the needle all the way to the front of the needle carrier assembly clicks into position 10 Re install the tray s in the tray base 11 Start the instrument 12 Close the front
101. m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m 1260 Infinity Bio inert Fraction Collector 181 11 Identifying Cables Analog Cables o One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog 182 1260 Infinity Bio inert Fraction Collector Agilent Module to BNC Connector Identifying Cables 11 p n 8120 1840 Pin BNC Pin Agilent Signal Name module a bed A Shield Shield Analog cL Center Center Analog Agilent Module to General Purpose p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog Red Analog a f 1260 Infinity Bio inert Fraction Collector 183 11 Identifying Cables Remote Cables 184 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to b
102. ment It might be more convenient to remove the needle from its carrier before unscrewing the needle tubing WARNING ie There is a risk of personal injury because the needle arm can move during fraction collector operation gt To avoid personal injury keep fingers away from the needle area 1260 Infinity Bio inert Fraction Collector 143 9 Maintenance Exchanging the Needle Capillary Carrier Assembly 1 Use the 4 mm wrench and the 5 16 wrench for 2 Hold the needle capillary guiding assembly between your counter holding to unscrew the valve to needle tubing thumb and forefinger and slide out the assembly towards from the needle assembly Viewed from the bottom the rear of the needle carrier assembly S f on gt i 4 V FEA 3 Un clip the tubing or capillary from the bottom of the 4 Slide the tubing or capillary through the hole in the carrier needle carrier assembly assembly from bottom to top and out of the holder in the z arm assembly 144 1260 Infinity Bio inert Fraction Collector Maintenance Exchanging the Needle Capillary Carrier Assembly pin slot fit It is only oriented and installed correctly three holding screws The carrier is held in position with a when pushed all the way up to the top assembly and unscrew them viewed from the bottom 5 Unplug the blue connector of the brown flat band cable of 6 Locate the three holding screws of the
103. message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system Leak Sensor Short Error ID 0082 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Please contact your Agilent service representative 2 Leak sensor incorrectly routed being Please contact your Agilent service representative pinched by a metal component 102 1260 Infinity Bio inert Fraction Collector Error Information 7 Leak Sensor Open Error ID 0083 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak senso
104. min DAD MWD working mode Threshold slope Upslope 5 mAU s Downslope 10 mAU s Threshold Upper threshold 10 mAU 3000 mAU 82 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Check out Procedures Expected Result Run a single injection under the conditions as described in Table 13 on page 82 You should observe a chromatogram with four peaks as displayed in Figure 25 on page 83 Four fractions should have been collected in the fraction collector vials NOTE The retention times of the peaks could be different depending on the system configuration e g flow cell type capillary volumes type and age of column Ready 10 0033 min 0 198 zec 2141 datapoints Figure 25 Chromatogram of the check out injection with UV based trigger 1260 Infinity Bio inert Fraction Collector 83 4 Using the Fraction Collector Application Notes More information about the Agilent 1260 Infinity fraction collectors and purification systems are available from a of application notes Printed versions can be ordered from Agilent or pdf files can be downloaded from the Agilent Website http www chem agilent com Table 14 Selected Agilent Technologies Application Notes Title Publication Number An optimized system for analytical and preparative work 5988 9649EN Recovery collection with the Agilent LC purification system 5988 9650EN Solutions for preparative HPLC Application Co
105. move and re insert the complete tray Otherwise the fraction collector will not recognize that the fraction containers were emptied Pooling When pooling fractions overfill protection no longer exists It is the user s responsibility to make sure that all fraction collection locations are large enough to completely collect all pooled fractions If a fraction collection location is overfilled an error message occurs and the pump is shut OFF 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Check out Procedures The purpose of the check out injection is the verification of the correct installation and configuration of the Agilent 1260 Infinity Purification System In addition the checkout procedure can be used for troubleshooting if the Agilent 1260 Infinity Fraction Collector doesn t operate as expected The checkout procedure depends on the flow range and on the peak trigger UV based or mass based In the following sections the check out procedures for the UV based trigger are described For the mass based system refer to the Agilent 1260 Infinity Purification System User s Guide p n G2262 90011 Check out Procedure for UV triggered Fraction Collection AS Prerequisites V The Agilent 1260 Infinity LC has been flushed thoroughly with the liquid phase as described in Table 13 on page 82 and the lamp s of the UV detector have been switched on for at least 15 minutes to provide a stable baseline
106. mpendium 5988 9646EN Innovative fraction collection with the Agilent LC purification 5988 9250EN platform Automated fraction re analysis does it really make sense 5988 8653EN Injection of high concentration samples with the Agilent LC 5988 8654EN purification system Sophisticated peak based fraction collection working with up and 5988 7895EN down slope Strategies for purification of compounds from non baseline 5988 7460EN separated peaks Method scale up from analytical to preparative scale with the 5988 6979EN Agilent LC purification system PS Peak based fraction collection with the Agilent LC purification 5988 5747EN system AS Influence of delay volume on recovery 84 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Solvent Information Observe the following recommendations on the use of solvents e Follow recommendations for avoiding the growth of algae see pump manuals e Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters e Avoid or minimize the use of solvents that may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Solvent information for parts of the 1260 Infinity Bio inert LC system For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality mat
107. n Description Definition Limits Rows Number of rows on the plate up to 16 Columns Number of columns on the plate up to 24 Volume Volume in pl of a sample vessel A Row distance Distance in mm between the center of two rows B Column distance Distance in mm between the center of two columns C Plate length X size in mm at the bottom of the plate 127 75 0 25 mm SBS Standard 1260 Infinity Bio inert Fraction Collector 47 3 Installing the Fraction Collector Table 6 Configuring Well plate Types Location Description Definition Limits D Plate width Plate height Column offset Row offset Column shift Well depth Well X size Well Y size Bottom size Y size in mm at the bottom of the plate Size in mm from the bottom to the top of the plate If you use well plates with inlets caps and septa you have to specify the distance from the bottom to the top of the caps Distance in mm from the left edge bottom to the center of the first hole A1 Distance in mm from the back edge bottom to the center of the first hole A1 Offset in mm to Y when the rows are not straight but staggered Distance in mm from the top of the plate to the bottom of the well If you use well plates with inlets caps and septa you have to specify the distance from the top of the septa to the bottom of the inlets Size of the well in x direction Plate length If you use well plates with
108. n completed the column instrument must be powered up again in order to store the values in the non volatile memory Table 21 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 Rs 232c 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up 1260 Infinity Bio inert Fraction Collector 203 12 Hardware Information 204 Table 22 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 3 4 5 0 0 9600 1 0 0 9600 0 0 1200 1 0 1 14400 0 1 2400 1 1 0 19200 0 1 4800 1 1 1 38400 Table 23 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 1 7 Bit Communication 8 Bit Communication Table 24 Parity Settings without on board LAN Switches 7 8 0 0 0 1 1 1 Parity No Parity Odd Parity Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity 1260 Infinity Bio inert Fraction Collector Hardware Information 12 Special Settings The special settings are required for specific actions normally in a service case Boot Resident Firmware update procedures may require this mode in case of firmware loading er
109. n to that module Figure 37 Configuration switch settings depend on configured mode All modules without on board LAN e default should be ALL DIPS DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 e DIP 1 DOWN and DIP 2 UP allows special RS 232 settings e for boot test modes DIPS 1 2 must be UP plus required mode For normal operation use the default best settings Switch settings provide configuration parameters for serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN 1260 Infinity Bio inert Fraction Collector Hardware Information 12 The following tables represent the configuration switch settings for the modules without on board LAN only Table 20 8 bit Configuration Switch without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 RSVD SYS RSVD RSVD FC The LAN settings are done on the LAN Interface Card G1369B C Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has bee
110. ncy emissions from the instrument itself 1260 Infinity Bio inert Fraction Collector 13 1 introduction Instrument Layout 14 1260 Infinity Bio inert Fraction Collector 1260 Infinity Bio inert Fraction Collector ee 2 Site Requirements and Specifications e i e j Site Requirements 16 Physical Specifications 19 Performance Specifications 20 This chapter provides information on environmental requirements physical and performance specifications gy Agilent Technologies 15 2 Site Requirements and Specifications Site Requirements Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 2 on page 19 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable t
111. ne 100 Pack 1260 Infinity Bio inert Fraction Collector 169 10 Parts for Maintenance Recommended Plates and Closing Mats Warnings WARNING Explosive gas mixtures There is a risk of explosive gas mixtures in the instrument if flammable solvents are used gt Cover the plates gt Remove the plates from the fraction collector after turning it OFF gt Only use solvents with a flash point higher than 200 C WARNING Contamination with adhesives Closing mats with adhesives can give some contamination in the system The adhesive is soluble in most of the solvents used in HPLC gt In general do not use closing mats with adhesive The fraction collector has no prepunch needle therefore the adhesive will clog the needle after several injections 170 1260 Infinity Bio inert Fraction Collector Parts for Maintenance 10 List of Recommended Plates and Closing Mats Recommended Plates and Closing Mats Std Well Plates and Closing Mats for Use with the Analytical Scale Fraction Collector only p n 5042 1386 5042 1385 5042 6454 5065 4402 5188 5321 5042 1388 5042 8502 G2255 68700 5022 6539 5022 6538 5042 1389 Description 96 well plate 0 5 ml PP pack of 10 96 well plate 0 5 ml PP pack of 120 96DeepAgilent31mm 96CappedAgilent Glass inserts 0 35 ml 1000 Pack 384Agilent 96Agilent conical Vial plate for 54 x 2 mL vials 6 pk Vial plate for 15 x 6 mL vials 1 pk Vial plate for 27 Eppen
112. needle It is approximately 23 ul Delay volume Vp which is specified in the Fraction Collector Configuration window is determined using the Delay Volume Calibration feature of the ChemStation software When a peak is detected during a purification run Figure 14 on page 59 the diverter valve is triggered using the following delay time calculations e Start of fraction collection t t tp End of fraction collection t tg tp tpe 58 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 0 1 2 3 4 5 6 7 8 9 min Figure 14 Chromatogram with peak start tg and peak end tg Detector Signal Delay Every Agilent 1260 Infinity detector that is used for triggering fractions has an internal signal delay caused by filtering the raw data The signal delay depends on the Peakwidth setting of the detector and is accounted for when the diverter valve is triggered Table 8 on page 59 to Table 12 on page 61 list the internal signal delay times for different Peakwidth settings Table 8 Signal Delay Times for the Agilent 1260 Infinity DAD MWD G1315D G4212B G1365D Peakwidth min Response time sec Signal delay sec lt 0 01 0 1 0 05 gt 0 01 0 2 0 15 gt 0 03 0 5 0 5 gt 0 05 1 0 1 25 gt 0 10 2 0 2 75 gt 0 20 4 0 5 9 gt 0 40 8 0 11 9 gt 0 85 16 0 23 9 1260 Infinity Bio inert Fraction Collector 59 4 60 Using the Fraction Collector Table9 Signal Delay Times for the Agilen
113. needle carrier VA WY ASA NNA y pa 2 x DI SS the needle carrier assembly from its plug at the z arm 7 Slide out the old needle carrier assembly to the bottom 8 Install the new carrier assembly and secure it with its es 145 1260 Infinity Bio inert Fraction Collector 9 Maintenance Exchanging the Needle Capillary Carrier Assembly 9 Plug in the blue connector of the brown flat band cable of the needle carrier assembly to its plug at the z arm 10 Slide the valve to needle tubing assembly through the hole in the z arm and out on the bottom of the needle carrier assembly top to bottom 11 With the needle held underneath the needle carrier assembly and using the 4 mm wrench and the 5 16 wrench for counter holding connect the valve to needle tubing to the needle Viewed from the bottom SeN PLIS 12 Insert the needle or capillary guiding assembly into the holder of the new needle carrier assembly Make sure to push it all the way to the front clicks into position 146 1260 Infinity Bio inert Fraction Collector Maintenance 9 Exchanging the Needle Capillary Carrier Assembly 13 IMPORTANT Clip the tubing or capillary into the guide on the bottom of the needle carrier assembly It is absolutely vital that the tubing is installed as described to maximize the lifetime of the tubing Next Steps 1
114. network cable shielded 7 m for point to point connection 1260 Infinity Bio inert Fraction Collector 209 12 Hardware Information Optional Interface Boards 210 1260 Infinity Bio inert Fraction Collector 1260 Infinity Bio inert Fraction Collector 13 Appendix General Safety Information 212 General Safety Information 212 Safety Standards 212 Operation 212 Safety Symbols 214 The Waste Electrical and Electronic Equipment Directive 215 Lithium Batteries Information 216 Radio Interference 217 Sound Emission 218 Agilent Technologies on Internet 219 This chapter provides addition information on safety legal and web gy Agilent Technologies 211 13 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for prote
115. ng hurt by the needle during operation gt Keep fingers away from the needle area during fraction collector operation Do not attempt to insert or remove a vial or a plate when the needle is positioned The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed CAUTION Damage of module electronics Connecting or disconnecting the fraction collector to thermostat cable as long as the power cords are connected will damage the electronics of the modules gt Before connecting the cable between the fraction collector and the thermostat unplug the power cords of the fraction collector and the thermostat 1260 Infinity Bio inert Fraction Collector 35 3 Installing the Fraction Collector CAUTION Damage through condensation If the condensation tube is located in liquid the condensed water cannot flow out of the tube and the outlet is blocked Any further condensation will then remain in the instrument This may damage the instruments electronics gt Make sure that the condensation tube is always above the liquid level in the vessel CAUTION Overheating of the module Insufficient air circulat
116. o location Depth 5 0 mm above location Distance 2 0 mm Continuous flow contact control Distance 0 0 mm rWell Plates Reserved Locations Plate Type Treat as m 4x96 Installed Plates Row s Plate 1 front left Plate 3 front right Column s I Plate 2 back left Plate 4 back right Hei Location s Figure 13 Fraction Collector Configuration dialog Use for Sample Recovery This checkbox only appears if multiple fraction collectors are configured see Figure 24 on page 78 Then the last fraction collector can be selected for sample recovery If four fraction collectors are configured the fourth one will automatically be used for sample recovery To change the order of your fraction collectors select Configure 1260 Access from the Instrument menu In the Configuration dialog box use Up or Down to change the configuration order and restart your ChemStation 1260 Infinity Bio inert Fraction Collector 53 4 54 Using the Fraction Collector Fraction Delay Volumes The Fraction Delay Volume specifies the volume between the detector cell and fraction collector diverter valve This volume has to be determined during the delay calibration See Delay Volumes and Delay Calibration on page 58 for a detailed description Collection Order The Collection Order describes the way of capillary movement during fraction collection Four different settings are possible R
117. o the instrument while the covers are removed CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 16 1260 Infinity Bio inert Fraction Collector Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of
118. odule firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module 1260 Infinity Bio inert Fraction Collector 1260 Infinity Bio inert Fraction Collector 10 Parts for Maintenance Fraction Collector Main Assemblies 164 Supported Trays for a Fraction Collector 166 List of Recommended Vials and Caps 167 Recommended Plates and Closing Mats 170 Fraction Collector Accessory Kit 172 Fraction Collector Thermostat 173 Needle Assembly 174 Diverter Valve Assembly 175 Tubing Kits 176 Internal Tray Assembly 177 This chapter provides information on parts for maintenance ee Agilent Technologies 163 10 Parts for Maintenance Fraction Collector Main Assemblies 164 Item p n 1 gt wo N G5664 60901 G1364 63114 G1367 47200 G1329 43200 G5667 87200 G1364 60022 G1351 68701 Description 3 2 Way valve assembly bio inert Internal tray analytical scale Plug channel Adapter air channel Needle assembly bio inert Needle carrier assembly analytical scale Interface board BCD with external contacts and BCD outputs OPTIONAL For bio inert modules use bio inert parts only Linert 1260 Infinity Bio inert Fraction Collector Parts for Maintenance 10 Fract
119. of the transport assembly Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Needle to Needle Rinse Funnel Position Failed Error ID 4955 4980 4981 4990 The needle failed to reach the needle rinse funnel position The position of the needle is monitored by a position encoder on the needle carrier If the needle fails to reach the end point or if the encoder fails to recognize the needle carrier movement the error message is generated Probable cause 1 Bad sample transport unit alignment 2 Bent needle 3 Missing needle 4 Blocked rinse or funnel position 5 Defective needle carrier assembly 6 Disconnected needle carrier connector 7 Defective MTP main board Suggested actions Do a self alignment Check and exchange the needle assembly if necessary Check and exchange the needle assembly if necessary Clean or change the funnel or rinse port assembly if necessary Exchange the needle carrier assembly Connect needle carrier connector correctly Please contact your Agilent service representative 1260 Infinity Bio inert Fraction Collector Needle Carrier Failed Error ID Error Information 7 The needle carrier on the transport unit assembly failed to move correctly Probable cause 1 Defective position sensor in the needle
120. on page 196 for details Optional Interface Boards p n Description G1351 68701 Interface board BCD with external contacts and BCD outputs 2110 0004 Fuse for BCD board 250 mA The BCD board provides a BCD output for the bottle number of the Agilent 1200 Series autosampler and four external contacts The external contact closure contacts are relay contacts The maximum settings are 30 V AC DC 250 mA fused Board identification 12 Processor BCD BCD interface register connector Line driver RFI filter 250mA External 4x External contacts lt contact connector DRF filter 206 1260 Infinity Bio inert Fraction Collector Hardware Information 12 There are general purpose cables available to connect the BCD output see BCD Cables on page 187 and the external outputs see External Contact Cable on page 190 to external devices Table 27 Detailed connector layout 1200 Pin Signal name BCD digit 1 BCD 5 20 2 BCD7 80 3 BCD 6 40 4 BCD 4 10 5 BCD 0 1 6 BCD 3 8 7 BCD 2 4 8 BCD 1 2 9 Digital ground 10 BCD 11 800 11 BCD 10 400 12 BCD 9 200 13 BCD 8 100 15 5V Low 1260 Infinity Bio inert Fraction Collector 207 12 Hardware Information 208 LAN Communication Interface Board The Agilent modules have one optional board slot that allows to add an interface board to the modules Some modules do not have this interface slot Refer to Interfaces on page 196 for det
121. ontact your Agilent service representative 2 Dirty or defective sensor Please contact your Agilent service representative 1260 Infinity Bio inert Fraction Collector 105 7 Error Information Module Error Messages Exhaust Fan Failed Error ID 4456 4457 The exhaust fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain value the error message is generated and the module shuts down Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Front Door Error Error ID 4750 The front door and or the SLF board are damaged Probable cause Suggested actions 1 The flat ribbon cable from MTP main Please contact your Agilent service representative board to the SLF board is not connected correctly 2 The sensor on the SLF board is Please contact your Agilent service representative defective 3 The door is bent or the magnet is Change the side door misplaced broken 4 Defective MTP main board Please contact your Agilent service representative 106 1260 Infinity Bio inert Fraction Collector Error Information 7 Side Door Error Error ID 4750 The side door and or the MTP board are damaged This error message is not displayed
122. or table contains seven columns Working Mode For each peak detector Threshold only Threshold Slope or Slope only are possible In the Threshold only mode the settings for Up Slope Down Slope and Upper Threshold in the subsequent columns are ignored Fraction collection is triggered whenever the detector signal exceeds the specified threshold value When the signal drops below the threshold value fraction collection is stopped In the Slope only mode fraction collection is triggered on the slope of the detector signal Adequate values for Up Slope and Down Slope can be specified in the corresponding fields In the Threshold Slope mode fraction collection is triggered on the corresponding values for threshold and slope The fraction collection is started if the detector signal exceeds both the threshold and the Up Slope value The fraction collection is stopped if the detector signal drops either below the threshold or the Down Slope value To specify the trigger values Up Slope Down Slope Threshold and Upper Threshold we recommend to use the Fraction Preview tool as described in Fraction Preview on page 67 Upper Threshold At high absorbance values the light intensity on the detector is extremely low and consequently detector noise will be superimposed on the detector signal In this case the detector noise might trigger fraction collection To avoid false fraction collection triggering we recommend setting an Upper Threshold well
123. ow by row Column by column Shortest path by column Shortest path by row Shortest path by row column is recommend if Continuous flow is selected as Collection Mode Collection Mode The Collection Mode defines the triggering of the diverter valve For Discrete fractions the diverter valve switches to waste while the needle moves to the next test tube vial or well For Continuous flow the diverter valve is not switching during needle movements except for long movements in the Row by row or Column by column mode In addition the needle cannot move into location during Continuous Flow operation Continuous flow is only available for well plates Needle Movement Into location In the Into Location mode the needle tip moves into the well to the specified Depth in mm This option is only available for the analytical scale fraction collector in the Discrete fractions mode Above location In the above location mode the needle tip stays at the specified Distance in mm above the well during fraction collection Contact control In this mode the needle tip moves down to the well bottom until is reaches the specified Distance in mm between needle tip and the vial well bottom This ensures that the forming droplet is in contact to the vial well 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 bottom During the continuing filling process the needle tip moves upwards while staying in contact with the surface
124. r 198 1260 Infinity Bio inert Fraction Collector Hardware Information 12 RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for e 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table 18 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI 1260 Infinity Bio inert Fraction Collector 199 12 Hardware Information 200 Instrument PC DCD 1 1 DCD RX 2 et 2 RX TX 3 X 3 Tx DTR 4 Sf 4 DTR GND 5 y 5 GND DSR 6 6 DSR RTS 7 V 7 RTS cis 8 slias RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 36 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent
125. r not connected to the main Please contact your Agilent service representative board 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being Please contact your Agilent service representative pinched by a metal component Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative 1260 Infinity Bio inert Fraction Collector 103 7 104 Error Information Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the main board in the module has failed short circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board
126. ray up or down see Figure 4 on page 33 13 Ensure the power switch on the front of the fraction collector is OFF and the power cables are disconnected 14 Connect the cable between the fraction collector and the thermostat see Figure 8 on page 39 15 Connect the power cables to the power connectors 16 Connect the CAN cable to other Agilent 1260 Infinity modules 17 If an Agilent ChemStation is the controller connect the LAN connection to the LAN interface 18 Connect the APG remote cable optional for non Agilent 1260 Infinity instruments 19 Turn ON power by pushing the button at the lower left hand side of the fraction collector 1260 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 Installing a Thermostatted Fraction Collector 20 The exhaust fan will turn ON and remove potential solvent vapor from the inside of the instrument After 2 minutes close the front door Then the fraction collector will start the hardware initialization process At the end of this process the status LED should be green 21 The fraction collector is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The fraction collector is turned OFF when the line power switch is protruding and the green light is OFF 000000 l eae AS 232 CAN CAN Fraction collector thermostat cable
127. rds the rear of the needle carrier assembly lt 141 9 Maintenance Exchanging the Needle Assembly 3 Insert the new needle assembly into the holder of the 4 Use the 4 mm wrench and the 5 16 wrench for needle carrier assembly Make sure to push it all the way counter holding to connect the valve to needle tubing to to the front Reassemble the wellplate adapter the needle Reassemble the wellplate adapter Viewed from the bottom Next Steps 5 IMPORTANT After fixing the needle in the needle carrier clip the tubing into the guide on the bottom of the needle carrier assembly in case it slipped out of this guide It is absolutely vital that the tubing is installed as described to maximize the lifetime of the tubing Re install the tray s in the tray base 7 Start the instrument Close the front cover 142 1260 Infinity Bio inert Fraction Collector Maintenance 9 Exchanging the Needle Capillary Carrier Assembly When If defective Tools required p n Description 8710 2438 Hex key 2 0 mm Parts required p n Description G1364 60022 Needle carrier assembly analytical scale Preparations Position the transport unit of the fraction collector in the Home position see Test Functions on page 117 Remove all installed trays from the tray base Position the transport unit of the fraction collector in the Change Parts position see Test Functions on page 117 and turn OFF the instru
128. ronic boards and components Switch OFF the fraction collector at the main power switch Disconnect cables from the interface board connectors Loosen the screws Slide out the interface board from the fraction collector F amp F O N Install the interface board Secure the screws 160 1260 Infinity Bio inert Fraction Collector Maintenance 9 Exchanging or Installing the Optional Interface Board 5 Reconnect the cables to the board connectors Figure 29 Exchanging the Interface Board 1260 Infinity Bio inert Fraction Collector 161 9 Maintenance Replacing Module Firmware When Tools required OR OR Parts required Preparations 162 The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or ifa new module with newer firmware is added to a system or if third part control software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Diagnostic Software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required m
129. rors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 25 Boot Resident Settings without on board LAN Mode Select sw1 sw2 sw3 SW4 SW5 SW6 swW7 Swe TEST BOOT 1 1 0 0 1 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 26 Forced Cold Start Settings without on board LAN Mode Select sw1 sw2 SW3 SW4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 0 0 1 0 0 1 1260 Infinity Bio inert Fraction Collector 205 12 Hardware Information Optional Interface Boards BCD External Contact Board The Agilent 1200 Infinity Series modules have one optional board slot that allows to add an interface board to the modules Some modules do not have this interface slot Refer to Interfaces
130. rt LC system on page 85 1260 Infinity Bio inert Fraction Collector 11 1 Introduction Early Maintenance Feedback 12 Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument
131. sk Fie Gactcs ltegation Caltvationy Report Sola Batch Pufy Y TRE Oe Joon arsenate 3 ei fea cal Lo e DADS A Sig 254 10 Ret 300 100 SCREENSICHECKOUTOOO00T 0 Information Fite Path C CHEMI2 1 DATA SCREENS Reason Date 03 Jan 06 1531 28 oao ooo Sample EX SF C Sample Irto 8A BSAA E A lt lt 1 0 Fraction table Figure 22 Fraction Task button In order to display the tick marks for the collected fractions on the screen click Signal options from the Graphics menu Then choose Separated in the Layout dropdown list 1260 Infinity Bio inert Fraction Collector 5 4 Using the Fraction Collector Viewing Your Results Report In order to create reports with a fraction table and tick marks the item Add Fraction Table and Ticks in the Specify Report box has to be checked Specify Report Instrument 1 x m Destination Quantitative Results l Printer V Screen Calculate 1 File Type Based On Area xl l File F Fw File Prefix F DIF r csv Sorted By Report LS T Ae Sith Signal Options Style Report Style Short x I Sample info on each page V Add Fraction Table and Ticks I Add Chromatogram Output l Add Summed Peaks Table Report Layout For Uncalibrated Peaks Separately With Calibrated Peaks C Do Not Report i Chromatogram Output Size Portrait of Page C Landscape 7 andscape Time jpo H Multi Page Landscape R
132. t 1260 Infinity DAD MWD SL G1315C G1365C Peakwidth min Response time sec Signal delay sec lt 0 0025 gt 0 0025 gt 0 005 gt 0 01 gt 0 03 gt 0 05 gt 0 1 gt 0 2 gt 0 4 gt 0 85 0 02 0 05 0 1 0 2 0 5 1 0 2 0 4 0 8 0 16 0 0 0375 0 0625 0 144 0 294 0 619 1 27 2 57 5 17 10 4 20 8 Table 10 Signal Delay Times for the Agilent 1260 Infinity VWD G1314B Peakwidth min Response time sec Signal delay sec lt 0 005 gt 0 005 gt 0 01 gt 0 025 0 05 0 1 0 2 0 4 lt 0 12 0 12 0 25 0 5 1 2 4 8 0 07 0 14 0 29 0 58 1 3 2 8 6 0 12 3 1260 Infinity Bio inert Fraction Collector Using the Fraction Collector 4 Table 11 Signal Delay Times for the Agilent 1260 Infinity VWD SL G1314C Peakwidth min Response time sec Signal delay sec lt 0 00125 gt 0 00125 gt 0 0025 gt 0 005 gt 0 01 gt 0 025 gt 0 05 gt 0 1 gt 0 2 gt 0 4 lt 0 02 0 02 0 05 0 12 0 25 0 5 o 0 0182 0 0364 0 0728 0 146 0 328 0 710 1 49 3 08 6 26 12 6 Table 12 Signal Delay Times for the Agilent 1260 Infinity FLD G1321A Peakwidth min Response time sec Signal delay sec lt 0 005 gt 0 005 gt 0 01 gt 0 03 gt 0 05 gt 0 1 gt 0 2 gt 0 4 lt 0 12 0 12 0 25 0 5 1 2 4 8 0 018 0 073 0 18 0 44 0 96 2 0 4 2 8 6 1260 Infinity Bio inert Fraction Collector 61 4 Using the Fract
133. tem stands on to avoid any back flow of solvent Re install the 6 Re install the tray s in the tray base 7 Start the instrument 8 Close the front cover 1260 Infinity Bio inert Fraction Collector 153 9 Maintenance Repairing or Exchanging a Funnel of the Internal Tray When When leaky or contaminated Parts required p n Description G1364 68730 Funnel seal kit pack of 10 G1364 43201 Funnel coupler G1364 86708 Waste tubing kit 0 5T analytical scale 5022 2200 Funnel Preparations Position the transport unit of the fraction collector in the Home position see Maintenance Functions on page 118 and remove all installed trays from the tray base Turn OFF the instrument 1 Remove the internal tray from the instrument see Exchanging the Internal Tray on page 152 eg ey 2 Turn the internal tray upside down and remove the funnel s waste tubing through the flow delay sensor 154 1260 Infinity Bio inert Fraction Collector Pana epa Maintenance ring or Exchanging a Funnel of the Internal T waste tubing through the flow delay sensor 1260 Infinity Bio inert Fraction Collector 3 Remove the funnel assembly from the internal tray by 4 Exploded view for the parts of the internal tray Once the screwing it counter clockwise and lifting it out funnel has been removed from the internal tray it can be disassembled and defective parts
134. the left side door take care of the magnet at the back Ensure the side door is correctly installed its presence is sensed by a hall sensor a missing side door will result in a NOT READY state of the instrument 7 Place the fraction collector in the stack or on the bench in all horizontal position 8 Ensure the power switch at the front of the fraction collector is OFF Connect the power cable to the power connector at the rear of the fraction collector 32 1260 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 Installing the Module 10 Connect the CAN cable to the other Agilent 1260 Infinity modules 11 If an Agilent ChemStation is the controller connect the LAN connection to the LAN interface should be installed to the detector 12 Connect the APG remote cable optional for non Agilent 1260 Infinity instruments 13 Install the tray that has been delivered for your fraction collector The test tube trays can be adjusted in height depending on the height of the used test tubes To adjust the height of the trays press against the snappers at the guides in the 4 corners of a tray and move the top plate of the tray up or down see Figure 4 on page 33 NOTE The Agilent 1260 Infinity Bio inert Analytical Scale Fraction Collector G5664A is shipped with two trays 40 x 2 ml half tray and 15 x 6 ml half tray All other trays have to be ordered separately Figure4 Adjusting the Height of the F
135. tion Snap Top Vial 2 mL clear glass 100 Pack Snap Top Vial 2 mL clear glass 1000 Pack Snap Top Vial 2 mL clear glass 100 Pack silanized Snap Top Vial 2 mL clear glass write on spot 100 Pack Snap Top Vial 2 mL clear glass write on spot 1000 Pack Snap Top Vial 2 mL clear glass write on spot 100 Pack silanized 1260 Infinity Bio inert Fraction Collector 167 10 Parts for Maintenance 168 p n Description 5182 0545 Snap Top Vial 2 mL amber glass write on spot 100 Pack 5183 4506 Snap Top Vial 2 mL amber glass write on spot 1000 Pack 5183 4509 Snap Top Vial 2 mL amber glass write on spot 100 Pack silanized Screw Top Vials Caps for Use with the Analytical Scale Fraction Collector only p n Description 5182 0714 Screw Cap Vials 2 mL clear glass 100 Pack 5183 2067 Screw Top Vial 2 mL clear glass 1000 Pack 5183 2070 Screw Top Vial 2 mL clear glass 100 Pack silanized 5182 0715 Screw Top Vial 2 mL clear glass write on spot 100 Pack 5183 2068 Screw Top Vial 2 mL clear glass write on spot 1000 Pack 5183 2071 Screw Top Vial 2 mL clear glass write on spot 100 Pack silanized 5182 0716 Screw Cap Vial 2 mL amber glass write on spot 100 pk 5183 2069 Screw Top Vial 2 mL amber glass write on spot 1000 Pack 5183 2072 Screw Top Vial 2 mL amber glass write on spot 100 Pack silanized Crime Caps Caps for Use with the Analytical Scale Fraction Collector only p n Descript
136. to be shipped to another location via carrier ensure V The transport assembly is in the park position Use the ChemStation or the Agilent Instant Pilot G4208A for this command To move the arm to the park position 1 Switch to the Diagnosis view of the ChemStation and select Fraction Collector gt Maintenance Positions from the Maintenance menu 2 In the upcoming dialog box click Park Arm V The vial tray and the sample transport mechanism is secured with the transport protection foam 1260 Infinity Bio inert Fraction Collector 1260 Infinity Bio inert Fraction Collector ee 4 Using the Fraction Collector Configuration of the Fraction Collector 52 Delay Volumes and Delay Calibration 58 Detector Signal Delay 59 Performing a Delay Calibration with an UV Detector 62 Setting up a Fraction Collector Method 63 Fraction Preview 67 Starting Your Run with Fraction Collection 69 Viewing Your Results 74 Special Applications 77 Limitations and How to Avoid Problems 80 Check out Procedures 81 Application Notes 84 Solvent Information 85 This chapter provides information on how to set up the module ott Agilent Technologies 51 4 Using the Fraction Collector Configuration of the Fraction Collector Configuration of the Fraction Collector in the ChemStation In order to setup or change the configuration parameters of your fraction collector select More Fraction Collector gt Configuration from the Instrument menu or
137. tware is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files The Instrument Utilities is a basic version of the Lab Advisor with limited functionality required for installation use and maintenance No advanced repair troubleshooting and monitoring functionality is included 1260 Infinity Bio inert Fraction Collector 95 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 96 1260 Infinity Bio inert Fraction Collector 1260 Infinity Bio inert Fraction Collector 7 Error Information What Are Error Messages 99 General Error Messa
138. u specify multiple injections for pooling the overfill protection is not activated If a fraction collection location is overfilled an error message occurs and the pump is shut OFF gt Make sure that all fraction collection locations are large enough to completely collect all pooled fractions 1260 Infinity Bio inert Fraction Collector 77 4 78 Using the Fraction Collector Sample Recovery The Agilent 1260 Infinity Fraction Collector offers different possibilities for sample recovery The preferred recovery strategy is to install multiple fraction collectors in your LC systems and use the last of those fraction collectors for recovery This recovery fraction collector can be selected in the Configuration dialog box as displayed in Figure 24 on page 78 The fourth fraction collector in your systems will always be used for sample recovery Also read Configuring Multiple Fraction Collectors on page 56 Fraction Collector 2 Configuration Instrument 1 E x Trays Tray A 40 Funnels M IV Use for Sample Recovery Tray B 40Funnels x Tube Volume ml 0 00 Tray C 40 Funnels x Tube Height mm jo Figure 24 Sample Recovery Fraction Collector For the following tray configurations fixed recovery location will be assigned from the ChemStation In order to disable the recovery the tray configuration has to be changed a Standard tray for two well plates 10 x 2 ml vials p n G2258 60011 and
139. ules in the sample leading to sample degradation and contamination gt For bio inert applications always use dedicated bio inert parts which can be identified by the bio inert symbol or other markers described in this manual gt Do not mix bio inert and non inert modules or parts in a bio inert system 128 The electronics of the module will not allow operation of the module when the top cover and the top foam are removed A safety light switch on the main board will inhibit the operation of the fan immediately Voltages for the other electronic components will be turned off after 30 seconds The status lamp will light up red and an error will be logged into the logbook of the user interface Always operate the module with the top covers in place 1260 Infinity Bio inert Fraction Collector Maintenance 9 Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 1260 Infinity Bio inert Fraction Collector 129 9 Maintenance Overview of Maintenance 130 The procedures described in this section can be done with the fraction collector in place in the stack These procedures can be
140. ull Test Tube 1260 Infinity Bio inert Fraction Collector 33 3 Installing the Fraction Collector Installing the Module 14 Turn ON power by pushing the button at the lower left hand side of the fraction collector 15 The exhaust fan will turn ON and remove potential solvent vapor from the inside of the instrument After 2 minutes close the front door Then the fraction collector will start the hardware initialization process At the end of this process the status LED should be green CAN cable to previous module sate whan prew orm we Configuration switch Control of thermostat Remote CAN DC Out RS232C AC Power CAN bus CAN cable to next module Figure 5 Cable Connections NOTE The fraction collector is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OFF 34 1260 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 Installing a Thermostatted Fraction Collector Parts required Preparations Description Fraction Collector and thermostat Chemstation or Agilent Instant Pilot G4208A Power cord For other cables see below and Cable Overview on page 180 Locate bench space Provide power connections Unpack the fraction collector and the thermostat Personal injury In the needle area there is a risk of beei
141. well plates can be used at a time in one tray 1260 Infinity Bio inert Fraction Collector 21 2 Site Requirements and Specifications Performance Specifications 22 1260 Infinity Bio inert Fraction Collector 1260 Infinity Bio inert Fraction Collector 3 Installing the Fraction Collector Unpacking the module 24 Optimizing the Stack Configuration 26 Installing the Module 30 Installing a Thermostatted Fraction Collector 35 Flow Connections to the Fraction Collector 40 Fraction Collector Trays 43 Configure Well plate Types 46 Transporting the Fraction Collector 50 This chapter provides information on unpacking checking on completeness stack considerations and installation of the module ee Agilent Technologies 23 3 Installing the Fraction Collector Unpacking the module Damaged Packaging CAUTION If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Deliver
142. y Checklist 24 Ensure all parts and materials have been delivered with the fraction collector For this compare the shipment content with the checklist included in each instrument box Please report missing or damaged parts to your local Agilent Technologies sales and service office The following models of Agilent 1260 Infinity Bio inert Fraction Collector are available e G5664A Bio inert Bio inert Fraction Collector designed for flow rates below 10 mL min well plates and a 40 funnel tray connecting to external locations of any size The G5664A can also be equiped with a thermostat G1330B 1260 Infinity Bio inert Fraction Collector Installing the Fraction Collector 3 Table4 Delivery Checklist for the Gb664A Bio inert Analytical Scale Fraction Collector Description Quantity Fraction collector module with inlet waste tubing assembly 1 Power cord local Matching the sockets in your country or region 1 Accessory kit see Fraction Collector Accessory Kit on page 172 1 Delay sensor calibrant p n G1946 85020 1 Table5 Delivery Checklist for the G1330B Thermostat Module optional Description Quantity Thermostat Module 1 Power cord local Matching the sockets in your country or region 1 Accessory kit thermostat module see Fraction Collector Thermostat on page 173 1 1260 Infinity Bio inert Fraction Collector 25 3 Installing the Fraction Collector Optimizing the Stack Configuration

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