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INFINITY ANALYZE User`s Manual

1. Fill Color Transparent DO Trailer Style Size Head Style Size 0 Show the color to draw the line Click to pick up a new color from the popup palette as illustrated below Page 111 Copyright 2008 GZ Lumenera Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 A ubomakic More Colors Push Automatic to specify the light gray color Push More Colors to bring up the Windows Color Picker Dialog for more and customized colors Show the width of line A new value may be entered directly Or use the spin buttons to adjust the setting Show the style of the line Click to pick up a new style from the popup style selector as illustrated below Ze Ee Ee Ee 8 KA A A A A A A E E Hf Ee E ee E E d E E ft E ee E FE d E E E Ee E E M T 77 Kall T T T T If checked the endpoints of L Round Pen the line will be drawn as solid balls Page 112 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual 3 9 3 Rectangle Use the Rectangle command to add rectangle objects Each rectangle is specified by two diagonal vertices Press down left mouse button to specify one vertex Move the mouse to the other vertex while keeping left mouse button pressed to draw the rectangle
2. INFINITY ANALYZE User s Manual SS Lumenera Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Page 2 Copyright Notice Release 4 6 0 Copyright 2008 Lumenera Corporation All rights reserved The contents of this document may not be copied nor duplicated in any form in whole or in part without prior written consent from Lumenera Corporation Lumenera makes no warranties as to the accuracy of the information contained in this document or its suitability for any purpose The information in this document is subject to change without notice License Agreement Software This Agreement states the terms and conditions upon which Lumenera Corporation Lumenera offers to license to you the Licensee the software together with all related documentation and accompanying items including but not limited to the executable programs drivers libraries and data files associated with such programs collectively the Software The Software is licensed not sold to you for use only under the terms of this Agreement Lumenera grants to you the right to use all or a portion of this Software provided that the Software is used only in conjunction with Lumenera s family of products In using the Software you agree not to a decompile disassemble reverse engineer or otherwise attempt to derive the source code for any Product except to the extent applicable laws specifically prohibit such restriction b rem
3. d Lu menera Copyright 2008 corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual When the active image window is showing the video the command actually rotates the live images 3 6 9 Magnification Use the Magnification command to synchronize calibration with objective switching For correct measurement and printing INFINITY ANALYZE needs to know the sampling intervals and magnification of the digital image Horizontal sampling interval vertical sampling interval and magnification comprise a micrometer Each image acquired with INFINITY ANALYZE has a micrometer associated with it This micrometer is the basis of correct measurement and printing and cannot be modified after the image has been created INFINITY ANALYZE also maintains a system micrometer When an image is being captured the system micrometer is duplicated and set as the micrometer of that image The process that sets up the system micrometer is referred to as calibration In the simplest sense calibration can be done for a single objective and use the Magnification command to adapt to different objectives or magnifications A successful calibration will correctly set up the sampling intervals and magnification for use with that objective When a new objective and or some other intermediate lenses are in place the Magnification command records the updated magnification and scales the sampling intervals accordingly System Micrometer Horz Gamp
4. All settings will be saved automatically for later use in the current and later sessions Copyright 2008 Lumenera corporation Page 28 d Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Image Layout Alignment O AAA All Images Automatically Aligned CAP Curent Image Absolutely Positioned CFP Current Image Fit to Paper oom for 444 and CAP Displacement for CAP mm Horz U Vert U Alignment This section determines whether all images or only the active image might be printed Three modes are defined by INFINITY ANALYZE AAA All Images Automatically Aligned CAP Current Image Absolutely Positioned and CFP Current Image Fit to Paper e AAA Under this mode all images except empty ones would be printed The layout and pagination of images are fully automatic A zoom factor may be applied to all images before the final page layout is calculated The zoom factor is specified in Zoom for AAA and CAP The titles would also be printed under images e CAP Under this mode the active image would be printed if it is not empty The size and position of the printed image may be modified The image may be zoomed according to the factor specified in Zoom for AAA and CAP The image can be placed anywhere on the paper The position of the printed image refers to the horizontal and vertical offset of the top left corner of the image with respect to the top left corner of the printable area
5. Targa File TGA Dr Halo CUT Amiga lFF IFFLBM__ TI JPEG Network Graphics JNG TI Fo a Format Zei me Graphics PCX Bitmap Format IPC TI Adobe Photoshop PSD SUN Raster File DAG TI Page 26 d Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Wireless Bitmap WBMP OO X11 Bitmap Format XBM TI X11 Pixmap Format EM J S O Metallograph Containing calibration and magnification information Note the native format sif preserves the most complete information for images acquired by INFINITY ANALYZE including sampling interval and magnification settings The Metallograph format meg also contains such information However the other formats do not contain metrical information and cannot be used for taking further measurements when re opened unless re calibrated When such a file is opened the sampling interval is assumed to be 1 micron and the magnification is assumed to be 100X 3 1 3 Close Use the Close command to remove the active image window lf the active image window is the only image window closing this window will cause INFINITY ANALYZE to switch to Main Frame Menu To go back to Document Menu choose File gt New for Video Preview to create an empty image window or choose File gt Open to load an image file 3 1 4 Save Use the Save command to store the active image onto disk in the native format lf the active image has been p
6. The first image is captured with a camera having defective pixels The second image is result of the operation The third image is the result of the command executed twice Page 86 Copyright 2008 Lumenera INFINITY ANALYZE 3 5 Unsharp Use the Unsharp command to do the generalized unsharp mask filtering for the active image The example below illustrates the process Copyright 2008 Page 87 GZ Lumenerg Corporation Lumenera INFINITY ANALYZE 3 7 6 Unsharp Options Use the Unsharp Options command to set up parameters for Unsharp Unsharp masking is a traditional film compositing technique used to sharpen edges in and improve the contrast of an image INFINITY ANALYZE generalizes this technique to make it more powerful The generalized unsharp masking also known as latitude reduction uses a Gaussian filter to separate an image into two images one is the low frequency part called background image and the other is the high frequency part called contrast image The background image representing the latitude or dynamic range of the image may be reduced by multiplying a coefficient smaller than unity Latitude reduction actually improves the contrast without enhancing noise If the image is free of noise the contrast image may also be multiplied by a coefficient greater than unity to augment contrast in a straightforward manner This process is referred to as high boost Finally there is a visual psychology f
7. VideoOn 0 Video Of Presets A preset consists of a complete set of parameters for controlling the camera a subset of capture options and image calibration information as detailed below Camera Settings Capture Options Frame Averaging Subsampling Calibration Information Sampling Intervals Micrometer Magnification INFINITY ANALYZE maintains a list of presets A preset is identified by a name and by its position in the list if the name is not unique A present name is a single line of text which is entered when a preset is created and added to the preset list as shown below Copyright 2008 Page 129 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Preset Hame 40X Fluorescent w OK A Cancel show the name of the preset i that is currently applied Click to apply another preset A special preset named Default will be created by INFINITY ANALYZE whenever a new camera Is attached It is always the first in the preset list and contains factory settings Push to remove the current preset from the preset list Note that the present camera settings capture options and system micrometer will not be affected Just that the preset is deleted from the list Add Push to create a new preset and add it to the preset list The name of the preset is specified in the dialog above The content of the preset is derived from the current camera settings capture options and s
8. 3 6 10 Micrometer gt CGalinraton 80 Page 6 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual 3 6 11 Micrometer gt Burn into Jmage 1cccccccseccnsecceeccescnsesccusccesenusssuseaussaeness 82 3 6 12 Micrometer gt Burn into Image Options 1ccccccseeeeesneeeeeeaneneseaneneenanees 82 3 THE ENHANCE MENU sessrererererenirtreNepeNE Sorene Eere NOREEN EEO rE Nore NON NON RDON ENEEK ESONRNS 83 3 7 1 FSA COCOON aa N E E eee 83 3 7 2 Flatfield Correction Options cccccccccsecccseeccseeccseecsseecsseecseeecsseessseeseeesenes 84 is A arc DOO EE 85 3 4 Remove Bad E 86 OLI UNS a eo E EAE ee en eee E N E E E E EE EEEE 87 3A UNNAD OOS E 88 3 7 7 Max Contrast ree cs ee ee 90 CNE e ORONO i EEA orcs EEA E E E E E EE 91 O79 MPE CIC ETa S a E E E 92 3 7 10 Eousgltzaton 93 OFT AmMplitud TCD C HON E 94 KEE E ee SINNU QUO ese era 95 3A E leie Hanso E 96 3 7 14 Spherical Aberration Correchon 98 3 7 15 Spherical Aberration Correction Options cccccceccceccceeccneesceescnseneeesseeees 99 3 8 JRE MEASURE MENU gue 99 3 8 1 LEE 100 38 2 Abo 100 AS hc A 0 BE 100 K Gi Ee 101 309 mmm le et Re TE 101 COI GE 103 387 ee sineren E ie sate Roem AEAEE 104 E Eege 105 IOF GETON eeh 106 3 8 10 Area erimeier 107 SETT OUDOT eE eE EE EE EAEE EE E O EETA NS 108 ET FONO EE 109 3 9 THE ANNOTATION MENU 109 3 9 1 TT AO EE 110 ERO PE e 110 3 9 3 Rectangle 1 2
9. ETS NHAGBEGZEid Control Panel A Imaging Control Preset Add White Balance ZZ eawB JO Capture BE Zo gt VideoOn 00 Video Off E Frequency s 0 Duration s o a xe x ommend Parameter cen quaweanseaw DIS D oe E Search in Caption Date Bone 2006 04 22 16 3 Tissue 2006 04 22 18 0 Inclusion 2006 04 23 16 1 u aseqeqeq F xd se we46oysIq PI LJ Page 14 Copyright 2008 Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual 2 1 5 The Empty Image Window INFINITY ANALYZE employs an empty image window to display live images from the camera The live image may pan and scroll in the same way as a still image i e the hand tool is also available for live image Use the Fit to Window icon to change between Full Field of View and a selected Region of Interest ROI for pan and scroll functionality A new preview window can be opened from the File menu or from an icon in the toolbar if the current one is closed Besides image windows many other windows or user interface elements reside within the main window of INFINITY ANALYZE These include menus toolbars status bar and auxiliary windows providing additional information about the images or control of the camera 2 2 1 Menu The menu can be either docked or floating Double click its grip or caption bar to toggle between the two states Whe
10. INFINITY ANALYZE User s Manual Release 4 6 0 po fll feld of view Subsamplirig Gi 02 03 O4 Sub sample the output image If 7 is selected no sub sampling will be done If 2 is selected the number of columns and number of rows of the image will be reduced by a factor of two by merging every two consecutive columns and rows 3 and 4 are similarly defined Subsampling reduces the noise level much like averaging The way INFINITY ANALYZE performs subsampling also helps to avoid aliasing Subsampling is performed only when capturing full field of view Pixel Shift Cameras Pixel shifting cameras such as INFINITYX 21 and INFINITY X 32 shift the sensor position from the Original location Use this option to specify the pixel shifting steps to use when completing an image capture To O File Field Group C Both select the destinations of the acquired image If File is selected the image will be stored to disk under that file path specified below If Field Group is selected the image will be appended to Field Group if there is still room If Both is selected the image will be stored as a file on disk and also appended to Field Group Show the file name to be used when the acquired image is to store on disk A new file name may be entered directly Push to bring out a File Save As dialog to specify the file name to be used when the acquired image is to store on disk Capture full field of view lf checked the ima
11. Release left mouse button to complete the creation of this rectangle Repeat the procedure above to add more rectangle objects A rectangle object has eight resizing handles as illustrated below Drag the handles at the four corners to adjust width and height of the rectangle simultaneously Drag the other handles to adjust width or height but not both Drag anywhere else within the rectangle to move the rectangle object Double click a rectangle to edit its properties As illustrated below the properties of a rectangle object consist of those properties of those four lines that make up the rectangle and the properties that specify how the interior of the rectangle is to be painted Copyright 2008 Page 113 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Properties Graphics Line Color E Style _ Round Fen i A Gize Fill Color _ Transparent DO Trailer Style Size Head Style Size 0 Release 4 6 0 For line properties please refer to the Line command The painting of the interior of a rectangle is defined by the Fill sector of the above dialog Fill Color e Transparent show the present color to fill the interior of the rectangle Click to pick up a new color as shown below Page 114 Copyright 2008 Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Automatic More Colors Click Automatic to cho
12. Selecting an image in Field Group 1 Click the thumbnail 2 Hover over the thumbnail for 2 or more seconds 3 5 10 Clear All Use the Clear All command to empty the Field Group Equivalent Command Right click the thumbnail to bring up Field Group Context Menu and choose Clear All Selecting an image in Field Group 1 Click the thumbnail 2 Hover over the thumbnail for 2 or more seconds 3 5 11 Show Previous Use the Show Previous command to display the currently selected image of Field Group in the active image window and then select the preceding image in Field Group For this command to work the selected image in the Field Group must have the same numbers of columns and rows of pixels and number of color channels as the active image otherwise the command fails quietly but the internal index will be decremented so next time the command is executed it can find the right image to display The Show Previous command replaces the image in the active image window by the selected image in the Field Group Note that only pictorial data are replaced and the other layers such as graphical overly are kept intact This facilitates the comparing of multiple images 3 5 12 Show Next Use the Show Next command to display the currently selected image of Field Group in the active image window and then select the following image in Field Group Copyright 2008 Lumenera corporation Page 64 d Lumenera INFINITY ANALYZE Relea
13. Should the unit fail during the warranty period Lumenera will at its option repair or replace the failed unit Repaired or replaced units will be covered under warranty for the remainder of the original one 1 year warranty period This warranty does not apply to units that after being inspected by Lumenera have been found to have failed due to customer abuse accidents mishandling tampering alteration improper installation improper power source negligence opening of the enclosure or if the serial number has been removed or damaged This warranty does not cover labor or incurred charges required in removing or installing the unit any business interruption loss of profits revenues or any consequential damages Units returned to Lumenera beyond the warranty period will be repaired if possible and all appropriate material and labor charges will apply Any returning product specifically those being returned under warranty must follow the Returned Material Authorization RMA process Any units being returned are to be properly packaged in Original packing if possible Lumenera will not cover damage sustained in shipping due to improper packing For RMA instructions please refer to our website at www lumenera com RoHS WEEE Compliance Statement The Restriction of Hazardous Substances in Electrical and Electronic Equipment ROHS Directive was passed into law by the European Union E U It affects manufacturers sellers
14. The maximal saturation allowable for the camera This is equivalent to adding 256 to the saturation of each pixel in a still image Hue Use the slider to adjust the hue of the camera Hue is J adjusted independently of saturation The minimal hue allowable for the camera This is equivalent to subtracting 128 from the hue of each pixel in a still image Show the current hue that is added to that of the images from the camera A new value may also be entered here Note that for a camera the hue is cyclic with a period of 360 The maximal hue allowable for the camera This is equivalent to adding 128 to the hue of each pixel in a still image Brightness Use the slider to adjust the brightness that is being added J to that of the images from the camera allowable for the camera 19 Show the current brightness that is being added to that of the images from the camera A new value may also be entered here The default value is 0 where the Page 140 d Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE brightness of raw images is not modified The maximal brightness 100 allowable for the camera Use the slider to adjust the contrast of the camera The minimal contrast allowable for the camera Show the current contrast of the camera A new value may also be entered directly here The default setting is 1 The maximal contrast 100 allowable for the camera The manage
15. added to a certain color component of all pixels A value may be entered directly Maximal allowable amount that can be added to a color component Push to close the dialog and finalize the adjustments to the active image Push to close the dialog and discard any modification to the active image lf the active image window is empty and is streaming video the nteractive command adjusts the gain saturation and hue of the camera Copyright 2008 Page 75 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Interactive Adjustment Intensity J v OK 1 25685 1 39063 A Cancel Saturation J Indicate the gain of the camera is being adjusted The gain of the camera is equivalent to a multiplier of the intensity channel of a still image Indicate the saturation of the camera is being adjusted The saturation aturnation i value 1 for the camera does not modify the images coming from the camera Hie Indicate the hue of the camera is being adjusted Note the hue of a camera is cyclic with a period of 360 That is adding 180 is equivalent to subtracting 180 The hue value 180 for the camera is equivalent to the hue value of 128 for a still image Use the slider to adjust the gain saturation or hue of the camera The minimal saturation applicable to the camera When the saturation of a color camera is set to 0 it delivers monochrome images Show the current saturation of the camera A
16. adjusted with the spin buttons The luminance or intensity is in the range 0 255 show the red of the currently selected color The red value can also be entered directly or adjusted with the spin buttons Page 52 Copyright 2008 Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual 0 255 ES Show the green of the Green 255 E currently selected color The green value can also be entered directly or adjusted with the spin buttons The green is in the range 0 255 show the blue of the currently selected color The blue value can also be entered directly or adjusted with the spin buttons The blue is in the range 0 255 Show the currently selected color Show the currently overlay color Push to dismiss the dialog and set the selected created color as the new color for displaying overlay Push to dismiss the dialog and discard any changes made during the dialog 3 3 Overlay Size Use the Overlay Size command to change the line width and font size for graphics generated by subsequent measurement operations All measurement operations generate graphical overlays Most of the measurement operations also generate textual annotation objects describing the quantitative output of the measurement In addition measurement may be carried out in either browsing mode or focusing mode of image display In browsing mode the full field of view is visible albeit possibly in a reduc
17. bmp JPEG pg Dr Hao cut Amiga IFF i om PO JPEG Network Graphics om Commodore 64 Koala Format koa oS Multiple Network Graphics mng SSS PCX Bitmap oe TI Portable Network Graphie png Adobe Photoshop oe Sun Rasterfile Ce TI Tara tg Wireless Bitmap wm X11 Bitmap Cum TI X11 Pixmap am TI Copyright 2008 Page 61 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 3 5 4 Export Use the Export command to save images in Field Group to disk The images in the Field Group will be saved in turn For each image a File Save As dialog is open with a default file name It is possible to change file name format and location on disk separately for each image The acceptable file formats are listed below Windows Bitmap bmp _ Independent JPEG Group Least compression applied TIFF Save as single page Portable Bitmap Save in binary format only Portable Network Graphics png Portable Pixelmap om o 3 5 5 Append Use the Append command to add the active image to the end of Field Group The Field Group is limited in both pre allocated memory and number of thumbnails When there is no room for another image the Append command fails quietly The Field Group retains all information of the image including metrical and magnification settings Equivalent Command Double click within the active image window 3 5 6 Load Use the Load command to
18. camera When cooling is activated the fan will be audible on the camera and the blue LED on the front of the camera housing will illuminate De select the checkbox to turn off the cooling function 4 1 3 Image Orientation Depending on the model of the camera being connected the live image may be rotated or flipped The image orientation i e rotation and flip may be controlled from this sub pane a Rotate degree clockwise Flip Horizontal _ Vertical Rotate degree clockwise Flip _ Horizontal _ Vertical Show the angle by which the live Show whether horizontal or image is currently being rotated vertical or both flips have been Click to specify a new value applied to the live image Click to set or reset a flip state Page 134 d Lu menera Copyright 2008 corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual 4 1 4 Capture Options Lapture Options Averaging GI 02 O04 Q8 Subsampling 1 O2 O3 Q4 To File Field Group Both C Documents and Settings Capture full feld of view Preview captured image Verify file name on capture Auto increment fle name Capture 16 bit file format Use alternate exposure 0 ms 10000 Averaging Select the number of frames GI O2 O04 O8 to be averaged If 7 is selected no frame averaging will be performed If 2 is selected two successive frames will be summed and then divided by two to create the inte
19. ccsccsscccsecccuccnseensusccescnsusnsuscassensusnsusesseessusnsusecesessusneusenseenaass 113 Bae POOO FROCIO ca wer nated rans A eee 115 KEREN 116 Choo EE tee 116 DF Ee 117 3 9 8 ES EE 119 3 9 9 EXO EE 120 EE EE 121 EOE EE 121 Co E 121 B G 13 Properties 00 cccccccc secs sccceecceecsuecsuecsuccsuessesseessuensuensueseessuessuensuessesseesseessees 121 Copyright 2008 Page 7 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Ta MOVETI TOD CHIC Sareea EEEE 122 3 9 15 Move to Lowermoet 1c cc ccececececcccecececececececececeneaeaeaeaeaeaeaeaeaeaeaeatataeaeaes 122 39 16 Move UPDET serris reir EEEa 122 3 9 17 EE 122 3 9 18 Burn miolmage 11s cseccseccneecsneeneecauecsueesauecsuessueessuessuessueesauessuessnesseesans 122 3 10 THE WINDOW MENU EE 123 OT ee EE 123 3 10 2 Tile Horizontally 0 eccceccsecccseccssccnsueccusccusensusceusesenensusssusesuensusssusessesnnssens 123 DAO TUOVA EE 123 SAQA ANANJO ICON soscas iiaii iniiai ir E TERETA E EEEE EE 123 Slk THE RELP MENU WE 123 ek beh E E a e E E E E E ener ners O E E 124 3 11 2 Database Geitigs 124 3113 Manual EE 126 PA ONO E e E 126 EK E eet 126 OTHER COMMANDS NEEN 127 4 1 IMAGING CONTROL N 127 dil CAO ME GOO E 129 Aba Fe e en E E E tet te eee ne eee se eee 132 AG WIV SC COC ANON E 134 7 a er COUE 8 0 6 lo ee eR en ee Pe Par enn nee nae ee ne en eee 135 4 1 5 Beeler EE 138 4 1 6 Camera Control Eviended ae raa Erias 139 DATABASE ccc
20. ends the measurement The caliper will be placed on the overlay layer of the image A short description showing the distance will appear in a text box annotation object A new entry will be appended to the auxiliary window Measurement to hold the value of the distance The example below illustrates the process Page 108 Copyright 2008 Lumenera INFINITY ANALYZE 3 8 12 Polygon Use the Polygon command to measure the area and perimeter of a polygon Click to specify the vertices of the polygon After the last vertex has been specified double click right button to start and to complete the measurement The polygon will be put on the overlay layer of the image A short text describing the measured area and perimeter will appear in a text box annotation object Two entries will be appended to the auxiliary window Measurement One entry is the area the other is perimeter An image of INFINITY ANALYZE may have temporary layers called annotation layers Each annotation layer consists of an annotation object An annotation object is a vector graphics object that can be moved resized or edited without loosing resolution Annotation layers exist only when an image is being annotated by INFINITY ANALYZE Annotation objects may be burned into the image where the pixels of the pictorial layer of the image are modified to represent the vector graphics of the annotation objects Annotation objects that have not been burned into image wh
21. image formation 3 1 14 Toggle Preview Use the Toggle Preview command to turn video streaming on or off INFINITY ANALYZE employs an empty image window to display live images Such a window is created automatically upon program startup To create a new empty image window choose File gt New for Video Preview The caption of the window indicates the model and serial number of the currently connected camera Only one empty image window can be used to preview video Choose Toggle Preview again to shutdown the video If it is desired to preview video in a particular empty image window make that image window the active image window and choose Toggle Preview Equivalent Commands From Capture Control Sub Pane Copyright 2008 Lumenera corporation Page 38 d Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual mh ah 400 Fluorescent wi Preset Add White Balance Auto Exposure a ArawWwe Capture VideoOn C Video Of gt Push to turn on live image preview on an empty image window If FI Video Un W there is currently no empty image window a new one will be created i video D Push to turn off live image preview 3 1 15 Capture Use the Capture command to acquire an image from the camera based on the present camera settings and capture options The camera settings include exposure time global gain color gains Gamma saturation hue brightness contrast image orie
22. mode INFINITY ANALYZE menu toolbars auxiliary windows except the Control Panel are hidden as illustrated below Copyright 2008 Lumenera corporation Page 30 d Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Pest White Balance AeawB je Capture gt VideoOn 1 Video Off in k Frequency s 0 Duration sl 0 Note that an enhanced print preview toolbar appears There are seven buttons the function of each is explained below i eprint kd OA 2 dose Click to start the actual printing Click to view the next page Click to view the last page Click to toggle between two page view and single page view Click to zoom in the image Click to zoom out the image Click to exit the print preview mode This is equivalent to pressing Esc key The status bar will display the page number of the image being previewed Page 31 Copyright 2008 DLLumenera Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 The main window shows the print layout of the images Print preview has incorporated configurations set by both Print Setup and Print Image Layout 3 1 9 Print Use the Print command to output a hardcopy of the active image or all opened images This command only initiates the printing process and does not specify how the printing should be done The printer selection and configuration is carried out by Print Setup The digital zoom factor position and align
23. open the selected image in Field Group A new image window will be created to hold a copy of the image in Field Group The image in Field Group will not be removed Equivalent Command Double click within the thumbnail of the image in Field Group Page 62 d Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Selecting an image in Field Group 1 Click within the thumbnail 2 Hover over the thumbnail for 2 or more seconds 3 5 7 Move Up Use the Move Up command to swap the selected image in Field Group with the preceding one Equivalent Command Right click the thumbnail to bring up Field Group Context Menu and choose Move Up Selecting an image in Field Group 1 Click the thumbnail 2 Hover over the thumbnail for 2 or more seconds 3 5 8 Move Down Use the Move Down command to swap the selected image in Field Group with the following one Equivalent Command Right click the thumbnail to bring up Field Group Context Menu and choose Move Down Selecting an image in Field Group 1 Click the thumbnail 2 Hover over the thumbnail for 2 or more seconds 3 5 9 Clear Use the Clear command to remove the selected image from the Field Group Equivalent Command Copyright 2008 Page 63 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Right click the thumbnail to bring up Field Group Context Menu and choose Clear
24. paper All measurements are done in microns and have nothing to Magniticatiar do with magnifying factor v K Push to close the dialog and commit the new settings of system micrometer e Push to close the dialog and GE discard any change to system micrometer IMPORTANT Calibration should be done on an image captured at Full Field of View with no subsampling 3 6 11 Micrometer gt Burn into Image Use the Burn into mage command to place a graphical representation of micrometer on the active image The graphical representation of the active image s micrometer will be placed on the overlay layer of the active image 3 6 12 Micrometer gt Burn into Image Options Use the Burn into Image Options command to customize the graphical representation of the micrometer Page 82 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Options for Burning Micrometer into Image Length 100 Ti Uk Text TOU micron A Cancel Margin Right bt Margin Botto b4 op The length of the scale bar in microns to be Length drawn on the overlay layer of the active image The text to appear under the scale bar ech BA The distance in pixels between the right side Margin Hight of the scale bar and the right side of the active image E4 The distance in pixels between the bottom Margin Bottom of the scale bar and the bottom of the active image options for burning micrometer in effect change to optio
25. referred to as the active image In this document the active image the current image or sometimes the active image window are used interchangeably Copyright 2008 Page 13 DXI Lumenera corporation Lumenera INFINITY ANALYZE The active image window can be easily distinguished from the other image windows The title of the active window is appended to the title of the main window and the title bar is highlighted When tabs are enabled for image windows the color of the tab of the active image is the same as the color of the inner frame of the main window as shown below in yellow The first example illustrates the active image window in normal state The second example illustrates the active image window in maximized state File Edit View Database Field Adjust Enhance Measure Annotate Window Help Dgan r a A u aA KNxKx Haan A IL L EEL i GE CR SEE a BE EAE Control Panel A Imaging Control Preset Add White Balance ZZ eawB JO Capture gt VideoOn 0 Video Off E Frequency s 0 Duration s o i cose quaweunseay E x D oe 3 Search in Caption Date 2006 04 22 16 3 Tissue 2006 04 22 18 0 Inclusion 2006 04 23 16 1 al co asegejeq GIS weaboysiy DI B LJ EE Infinity Analyze Video G8 File Edit View Database Field Adjust Enhance Measure Annotate Window Help DSH anjan Rea AX San e fa eee amp tE ORM
26. text box annotation object A new entry will be made to the auxiliary window Measurement recording the value of the radius Repeat the above procedure to measure more circles The example below illustrates the process Page 106 Copyright 2008 Lumenera INFINITY ANALYZE 3 8 10 Area Perimeter Use the Area Perimeter command to measure the area and perimeter of a closed shape The closed shape is created by freehand drawing i e moving the mouse while pressing down left mouse button Release the left mouse button to signify the end of drawing The starting point i e where left button is pressed and the ending point Le where the left button is released will be connected automatically by a straight line to close the shape The drawing will be put on the overlay layer of the image A short text describing the measured area and perimeter will appear in a text box annotation object Two entries will be appended to the auxiliary window Measurement One entry is the area the other is perimeter The example below illustrates the process Copyright 2008 Page 107 Lumenera INFINITY ANALYZE 3 8 11 Caliper Use the Caliper command to measure the distance between two points or two parallel lines Click to specify two points After the first click a rubber band with two perpendicular legs is being drawn to connect the first point and the current mouse position Use the legs to align with parallel lines The second click
27. used in a loose sense to describe wavelength distributions of the light sources Copyright 2008 Page 35 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 The camera itself or INFINITY ANALYZE cannot possibly determine the temperature of the light source from live images alone and has to rely on user input to generate the digital image that best matches human perception Light Source O Fluorescent Ce Daylight O Incandescent Menon gt Halogen Other Select to indicate to INFINITY ANALYZE that the light source has an interrupted spectrum 3 Daylight Select to indicate to INFINITY ANALYZE to add more red Fluorescent Select to indicate to INFINITY ANALYZE to add even more red ANALYZE to add more blue Select to indicate to INFINITY ANALYZE to add more red and blue Select to indicate to INFINITY Other ANALYZE no color temperature should be incorporated in image formation Push to close the dialog and keep the just specified light source temperature Push to close the dialog and ji discard any modification to light source temperature Page 36 d Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Equivalent Commands 1 From Settings File Upon startup INFINITY ANALYZE automatically loads and applies the light source color temperature setting used last time The color temperature
28. 9 DXI Lumenera corporation Lumenera INFINITY ANALYZE image will be modified by adding corresponding pixels from the contrast image The parameter determines the dependency of back projection on background image Push to dismiss the dialog and keep the parameters for Unsharp Cancel Push to dismiss the dialog and discard any change to parameters for Unsharp 3 7 7 Max Contrast Use the Max Contrast command to linearly scale the intensities of the active image so that they span the whole dynamic range The following two images together with their histograms illustrate the process Page 90 Copyright 2008 Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Histogram Histogram 3 7 8 Sketch Use the Sketch to generate a pencil drawing from the active image The example below illustrates the process Copyright 2008 Page 91 Lumenera INFINITY ANALYZE 3 7 9 Adaptive Edge Emphasis Use the Adaptive Edge Emphasis to enhance edges in the active image The example below illustrates the process Page 92 Copyright 2008 Lumenera INFINITY ANALYZE 3 10 Equalization Use the Equalization command to manipulate the histogram of the active image to make it as flat as possible The following two images together with their histograms illustrate the process Copyright 2008 Page 93 pLu nenera corporation Lumenera INFINITY ANALYZE User s Manual Relea
29. Aberration Correction Options command to specify to what degree the spherical aberration will be corrected Options for Spherical Aberration Correction More Clarity No Effect 100 Less Nose There is only one parameter to control the spherical aberration correction Acceptable values for this parameter are in the range 1 200 Setting the parameter to a number less than 100 will cause haze to be removed from the image Setting the parameter to a number greater than 100 will smooth the image The measuring operations of INFINITY ANALYZE are the digital version of the more traditional planimetry with microscopes A measuring operation of INFINITY ANALYZE generates one two or three pieces of output information Each operation results at least in some graphics on the overlay layer of the image Most operations also create textual annotation objects describing the quantitative outcome of the measuring operations and entries in the table of the auxiliary window Measurement holding the quantities Copyright 2008 Page 99 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 An image of INFINITY ANALYZE can have many layers At the bottom is the pictorial layer which is similar to an ordinary image consisting of pixels Above the pictorial layer is the mask layer The mask layer is useful to image segmentation and mathematical morphology The next layer is the overlay The micrometer when burned into image will be placed on this
30. ITY ANALYZE preset for example 400 X examines the most recently Fluorescent is selected the grabbed frame and adjusts camera will be directed to amplify the color channel gains of the each color channel of the video camera in an effort to stream by respective parameters reproduce the colors in the recorded in that preset best way The preset will be deselected to reflect the change 3 1 12 Manual White Balance Use the Manual White Balance command to adjust the gain setting used on each color channel to manipulate the color output of the camera This feature can be used to fine tune the color appearance of the camera image when necessary 3 1 13 Light Source Use the Light Source command to tune the color reproduction of the camera The same object may appear slightly different in color under different lighting conditions The reason for this change in camera response is due to the light source itself which can have varied chromatic content called temperature It is measured in Kelvin When describing a light source as having a Kelvin rating it refers to a light source that emits Page 34 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual energy across the entire visible range from 300nm to 700nm Sunlight and incandescent lamps fit to this model conveniently However all fluorescent and discharge lamps have interrupted spectra where Kelvin temperature is only
31. NFINITY ANALYZE Release 4 6 0 User s Manual Database Caption Bone Date o 42272006 w April 2006 7 4 85 E 7 6 11 q2 13 14 15 18 19 20 A Author responsible for the acquisition Jack processing or measurement of the active image Must be a single line of text Description Specify a single line of text as a note Radiograph processed as photomicrograph fiat or comment Push to add the new record to database Push to exit the dialog and do not modify the database in any way The user account must have write privilege on the folder where the database is located otherwise adding records to the database will not be successful Typically the database file is in the same location as the INFINITY ANALYZE application however it is also possible to specify an alternate file folder location for the database if required Copyright 2008 Page 143 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 4 1 8 Load Push to open the selected record in the database Note a record in the database must have been selected for this command to work Otherwise a warning message appears as the following Infinity Analyze A Please select a record When a record is successfully loaded a new image window will be created to hold the image data from that record If the image has been measured before measurement information will also be loaded into the auxiliary window Measureme
32. Sub Pane h Pan 400 Fluorescent w Preset Add White Balance Auto Exposure A Arawe 26 Capture Video On C Video Of 400 Fluorescent e Camera presets include averaging and decimation options lf a preset for Page 40 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual example 400 X Fluorescent is selected the camera will be directed to average incoming frames and decimate the averaged image according to settings recorded in that preset The Capture Options Sub Pane will be updated to synchronize with the current setting 3 From Capture Options Sub Pane Averaging 1 02 04 O8 Decimation Oi O2 O3 Gi To Fie Field Group C Both ehreportspecimer jpg aa Capture full feld of view _ Preview captured image _ Verify file name on capture Auto increment file name Averaging Select the number of frames to be averaged If 1 is 1 O2 04 Cp selected no frame averaging will be performed If 2 is selected two successive frames will be summed and then divided by two to create the intermediate image that is the input to subsequent post capture processing 4 and 8 are similarly defined Frame averaging reduces noise If n frames are averaged the energy of the noise will be 1 n of its Original level Frame averaging is performed only when capturing Copyright 2008 Page 41 DXI Lumenera corporation Lumenera
33. Y ANALYZE will load the screen Gamma setting used last time Upon exit INFINITY ANALYZE will store the screen Gamma value to a private settings file 2 From Help gt Settings Any change made to screen Gamma will be shown when this dialog is open Any change made to screen Gamma during this dialog will be effective when INFINITY ANALYZE runs next time Copyright 2008 Lumenera corporation Page 56 d Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual A full featured database system has been built into INFINITY ANALYZE The database is accessible whenever INFINITY ANALYZE is running All the information content of an image together with its quantitative result of measurement date of creation author and textual description may be archived in the built in database INFINITY ANALYZE uses a caption to uniquely identify each record in the database User specifies a caption for each image that is being stored to database Besides the caption there are three other fields in each record Date is the time of creation of the record Author is the name of the operator that has captured or processed the image Description is a single line of text usually explaining the image or the measurement performed over the image When a new record is created for an image the quantitative result of any measurement done so far for the image as shown in the auxiliary window Measurement will also be saved to database The built in dat
34. abase is fully controlled from the corresponding auxiliary window The menu duplicates some of the more frequently used commands 3 4 1 Store Use the Store command to save the active image and its measurement result to the built in database Database Caption Add Bone ior Date 4 22 2006 Author Jack Description Radiograph processed as photomicrograph Copyright 2008 Page 57 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Laption Specify a caption for the record to create Must be a single line of text The title of the active image is Initially displayed but it is free to specify a different one The caption should be unique within the database Otherwise a warning message appears as shown below Answer Yes to replace the existing record with the same caption Answer No to abort the storing operation Bone Infinity Analyze K Database already contains 4 record captioned Bonge Would you like to replace the record Date Specify the date of creation of the record e ele ce ara as illustrated below Database Caption E one Date O 4722 2006 na April 2006 4 5 E 7 6 11 12 13 14 15 18 193 20 A Page 58 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual po be a ingle line of text Description Specify a single line of text as a note remark or comment Radiograph processed as photomicrograph Pus
35. actor to be considered Boosted contrast in dark areas of an image may be interpreted as noise To make the processed image appear natural the intensities of background image pixels can be used as weights when the contrast image is being added back to the background image to form the output image of the latitude reduction operation The process of adding contrast image to the background image is called back projection Page 88 Copyright 2008 Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Options for Unsharp Mask Radius Latitude Reduction High Boost Combine TE The support of the Gaussian filter to separate the active image into background and contrast The coefficient by which the background image will be multiplied 0 5 corresponds to latitude reduction by 1 bit 0 25 corresponds to latitude reduction by 2 bits Any value may be entered here However if a number greater than 1 is given the contrast of the image will be decreased High Boost The coefficient by which the contrast image will be multiplied Any value may be entered here However if a number smaller than 1 is given the image will be smoothed Specify how the background image and contrast image are combined Valid values are in the range 0 1 If O is entered the background image and the contrast image will be simply added If 1 is entered only the brightest pixels of the background Latitude Reduction Copyright 2008 Page 8
36. age itself is being modified in any way including the way it is being displayed For a live image its histogram is updated every three seconds In both cases the histogram is only for the part of the active image that is visible on computer screen Look Up Table x T iy sy mafa a ry lt a WEBROOT The title bar of the histogram pane includes icons used to access Look up Table LUT functions When enabled the LUT is applied to the live preview window providing instant feedback based on the Red Green and Blue channel manipulations Each channel can be modified individually or all three channels can be set to be the same The LUT can be enabled or disabled as desired Use the File menu to access the option that applies the active LUT to a captured image The horizontal axis of the histogram represents the image input intensity values from minimum 0 on the left up to maximum 255 on the right The vertical axis represents Page 20 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual the corresponding output intensity value from minimum 0 at the base up to maximum 255 at the top The line displayed across the histogram represents the point at which an input intensity value is transitioned to the corresponding output intensity value Therefore a line sloping upwards from the bottom left corner to the top right corner represents a neutral LUT defini
37. al and the hue and saturation channels of HSI are set to zero 2 1 2 Raster Images and Vector Graphics The components that make up the scene on a computer screen fall into two main categories raster images and vector graphics Raster images use a grid of small squares known as pixels to represent pictures Each pixel in a raster image has a specific location and color value assigned to it When working with raster images groups of pixels rather than objects or shapes are being edited Because they can represent subtle gradations of shades and color raster images are the most common electronic medium for continuous tone pictures such as photomicrographs The terms raster image and pixel array are used interchangeably in this document In fact a raster image may also be described as a matrix of points The distance between neighboring points on a row is referred to as the horizontal sampling interval The distance between neighboring points on a column is referred to as the vertical sampling interval Sampling interval multiplied by the number of columns and rows is exactly the size of the field of view over which the image has been captured Vector graphics consist of lines and curves defined by mathematical objects called vectors which describe pictures according to their geometric characteristics The lines or curves of an object can be painted with a specific color The interior of the shape enclosed by these lines and curves may also b
38. ands for the half of the dynamic range i e 128 Another line being drawn below stands for 0 The curve drawn over the image stands for the intensities of the pixels Move the mouse while keeping the left button of the mouse pressed to preview other rows of pixels Release the left button of the mouse to finalize the profiling Repeat the above procedure for more profiles The Light Density command generates graphical output only The examples below illustrate the process Page 102 Copyright 2008 Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual 3 8 6 Counting Use the Counting command to simulate a tally machine Click to count A temporary mark appears with each click Right button double click to finish counting The visited locations will be marked with serial numbers These graphics appear on the overlay layer of the image The Counting command also generates an entry in the auxiliary window Measurement The example below illustrates the process Copyright 2008 Page 103 GZ Lumenera corporation Lumenera INFINITY ANALYZE 3 8 7 Angle Use the Angle command to measure angles Click to specify three controlling points The angle made by the line passing through the 1 and the 2 point and the line passing the 2 and 3 point will be measured The Angle command if successfully executed generates three types of output The angle will be shown as graphics on the overlay lay
39. ast of a stack of 29 images gathered over the same field of view and the image on the right is the fusion of the image stack 3 5 16 Math The Math functions provide the ability to perform operations on images stored in the Field Group 3 5 16 1 Combine Use the Combine command to compose a color image from the last three images in the Field Group Copyright 2008 Page 69 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 The last three images are assumed to be the red green and blue channels of a color image As such they must be identical in the numbers of columns and rows of pixels It is not required that these images are all color or all monochrome If a color image is in place only its first channel i e red will be extracted A new image window will be created to hold the output image The output image has the same metric and magnification setting as those of the last image in the Field Group 3 5 16 2 Split Use the Splitcommand to decompose a color image into three monochrome images representing its red green and blue channels The output images are appended to the Field Group 3 5 16 3 Subtract Use the Subtract command to subtract the active image from each image in Field Group For this command to work each image in the Field Group must have the same numbers of columns and rows of pixels and number of color channels as those of the active image The output images will replace those corresponding
40. ay be specified Copyright 2008 Page 119 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 object Enter the text for the Show how the Fees Border Elipse Lei rectangle of the text appears Click the drop list to specify whether the bounding rectangle is to be displayed Straight as a rectangle ora round rectangle or an ellipse or not to be displayed at all Er Show how the text is positioned in the bounding rectangle Click the drop list to specify whether the text is to be left justified right justified or to be placed in the center If the bounding rectangle is to be shown the line and fill properties apply to the bounding rectangle If the bounding rectangle is to be shown as rectangle full line properties apply If the bounding rectangle is to be shown as round rectangle or ellipse line style is forced to be solid and the other line style settings will be ignored 3 9 9 External Use the External command to insert an OLE object This command opens the standard Windows Insert Object Dialog as shown below All servers currently installed on the system are listed where an object may be created anew or from a file Page 120 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Insert Object Object Type Create New Microsoft Equation 3 0 Microsoft Graph Chart KE Microsoft Office Excel Chart O Create from Eile Micro
41. cecececececcececececeeeeeenecececeeeuaenenececeeeneuaeneneneceeetaeaeneneeeceveneeaeneneeevenenenennenenes 141 r m Oe Ee E 141 4 1 8 LOO ON 144 4 1 9 Ueieie 144 4 1 10 Reffesh E 145 a ON een E E E E E ee E AT 145 Copyright 2008 Lumenera corporation T d Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Introduction The INFINITY ANALYZE application package is designed to work exclusively with the Lumenera INFINITY camera models It provides all of the functions necessary to control all available camera settings for both the live video preview and the capture of still images In addition ANALYZE provides a comprehensive set of measurement and analytical operations useful in many clinical laboratory and inspection operations For details on the features and performance of a particular INFINITY camera model refer to the INFINITY CAPTURE Users Manual Copyright 2008 Page 9 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Getting Started It is assumed that the users of INFINITY ANALYZE and the readers of this document have a working knowledge of microscope digital camera and Microsoft Windows INFINITY ANALYZE may be used for both compound and stereo microscopes It also contains a few functions specifically designed for fluorescent imaging INFINITY ANALYZE conforms to the Microsoft Windows User Interface Guideline and so is similar to other programs in opening file
42. d rectangle i e only solid line style is applicable 3 9 6 Polygon Use the Polygon command to add polygon objects There are two ways to create a polygon object by clicks and by freehand drawing By Clicks A polygon is specified by its vertices Click to add a new vertex Double click to add the last vertex By Freehand Drawing Press down the left mouse button to start drawing Move the mouse while keeping left button pressed to draw a polygon Double click to finish drawing lf a polygon has been created by clicks each vertex is a resizing handle If a polygon has been created by freehand drawing each point on the drawing is a resizing handle See below for an illustration The polygon on the left is created by clicks the one on the right is created by freehand drawing Copyright 2008 Lumenera corporation Page 116 S Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual A polygon has an identical set of properties to that of an ellipse 3 9 7 Arrow Use the Arrow command to add arrow objects An arrow is made up of an arrow head an arrow trailer and a line connecting the arrow head and trailer The arrow head and arrow trailer are shapes consisting of enclosing lines and interiors The lines enclosing arrow head and arrow trailer and the line connecting the arrow head and arrow trailer have full line properties associated with them The interiors of arrow head and arrow trailer have full fill propert
43. d to hold the output panorama image The output image copies the sampling intervals and magnification setting from the first input image from Field Group Due to the overlapping of the input images the number of columns of the output image would be less than those of input images summed Due to possible vertical shift of the input images the number of rows of the output image might also be less than that of an input image The cropping is automatic and transparent to users The example below illustrates the process The first three images are stitched to create the forth image Copyright 2008 Page 65 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 3 5 14 Panorama Portrait Use the Panorama Portrait command to stitch a column of images The images to stitch should have been stored in Field Group and ordered correctly All images in Field Group participate in the operation These images must be identical in the numbers of columns and rows of pixels and the number of color channels The first image in Field Group should be the topmost image of the column of images to be aligned The last image in Field Group should be the bottommost image of the column of images to be aligned There must be at least two valid images in Field Group In addition any two adjacent images must have at least 20 non overlapping areas and at least 32 overlapping rows of pixels The images are allowed to shift horizontally but the shift has to be li
44. ders an image as a modified document if it has never been saved in native format or it has been processed or measured after the last time it was saved Viewing an image in different ways measurement in progress not yet completed and annotating an image before the annotation objects are burned into images are not considered as modifying an image Copyright 2008 Lumenera corporation Page 46 d Lumenera INFINITY ANALYZE INFINITY ANALYZE maintains a processing history for each image The processing history is a chain of internal buffers holding the states of the image A new state of the image is automatically created and appended to the processing history after each operation that has modified that image Viewing an image in different ways measurement in progress not yet completed and annotating an image before the annotation objects are burned into images are not considered as modifying an image 3 2 1 Undo Use the Undo command to reverse the most recent action that has modified the image The possible number of undo steps is the length of processing history 3 2 2 Redo Use the Redo command to undo an Undo operation 3 2 3 Copy to Clipboard Use the Copy to Clipboard command to convert a copy of the active image to Windows Bitmap format and place it on the clipboard The commands in this group are used to customize the appearance of user interface elements images and computer screen 3 3 1 Toolba
45. dification to the active image lf the active image is an empty image and the active image window is streaming video the Image Gamma command actually sets the Gamma value of the camera Image Gamma Ka wf DK K Cancel Use the slider to set the Gamma of the camera The minimal Gamma value applicable to the present camera 0 75 Show the current Gamma setting of the camera A value can also be entered directly The maximal Gamma value applicable to the present camera Copyright 2008 Page 73 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 ills Push to dismiss the dialog and keep the new Gamma setting for the camera Push to dismiss the dialog and discard any change to the camera settings during the dialog 3 6 2 Area Based White Balance Use the Area Based White Balance command to correct color aberration of the active image or the camera Correction of color reproduction is based on the values of a small rectangular neighbourhood of pixels These pixels should be colorless but instead are showing a tint INFINITY ANALYZE will calculate the relative strength of each color component and adjust the color gains accordingly Click a pixel within the active image to indicate to INFINITY ANALYZE the centre of the neighbourhood on which color correction will be based lf the active image window is displaying live images from the camera this command performs area based white ba
46. distributors and recyclers of electrical and electronic equipment containing lead cadmium mercury hexavalent chrome polybrominated biphenyl PBB and polybrominated diphenyl ether PBDE After July 1 2006 the use of these materials will be banned in new products sold in Europe The RoHS Directive complements the WEEE Directive China is expected to adopt similar legislation within a similar timeline The Waste Electrical and Electronic Equipment Directive WEEE aims to reduce the waste arising from electrical and electronic equipment and to improve the environmental performance of all those involved in the life cycle of these products Lumenera is committed to protecting people and the environment and we are working on identifying any materials used in our processes that could pose a potential hazard to our employees customers or the environment For this reason we are committed to have all our products comply with the RoHS and WEEE directives We are constantly improving our compliance with these directives For more information on our compliance or to track our progress please refer to our website Trademarks and Proprietary Names Lumenera Scientific INFINITY X INFINITY 1 INFINITY 2 INFINITY 3 INFINITY 4 INFINITY 5 and DeltaVU are trademarks of Lumenera Corporation This manual may refer to other product names which may be trademarked by their respective companies and are used herein for identification purposes only Copyrigh
47. e lapse capture one is the time interval at which the images will be acquired and the other is the span referred to as duration of the time which is needed for the whole sequence of images to be captured An image of the sequence will be acquired according to the camera settings and capture options at the time when the acquisition action occurs Each captured image will also be calibrated according to the system micrometer and sub sampling factor at the time when the acquisition action occurs Note that the time lapse capture initiated from this sub pane will be carried out in the background Other functions within INFINITY ANALYZE may still be accessible during the time lapse capture Time Lapse S Interval Is Duration Je merd sl Time interval in seconds Duration 3 Time duration in seconds Push to initiate time lapse capture Video preview might be necessary if not to capture full field of view Push to abort the time lapse capture 1 of 4 images OK 12s ta go The progress of the time lapse capture Both the number of captured images and the estimated time in seconds needed to complete the task are given The interval between sequential image captures will depend on the A performance of the camera In addition the ANALYZE application imposes a certain amount of processing overhead It may not be camera model and camera settings that dictate the frame rate possible to obtain images at a requested interval shorter
48. e magnification power of the objectives 3 6 10 Micrometer gt Calibration Use the Micrometer gt Calibration command to set up system micrometer Page 80 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual The system micrometer consists of the sampling intervals in horizontal and vertical directions and the magnification intended for printing this image In the calibration process only one of the two sampling intervals will be calculated as modern cameras all have square pixels INFINITY ANALYZE derives the sampling interval from the length of a straight line segment on the image of a calibration slide An image of calibration slide may be captured with INFINITY ANALYZE as illustrated below Click on the two endpoints to specify a line segment on the image of the calibration slide 30 40 e NW Te DU Micrometer Calibration Length 100 ue Magnification 100 AC Cancel iil In the Micrometer Calibration dialog specify the length of the line in microns and the intended magnification Micrometer Calibration Length Ti Magnification A Cancel Length a Enter the length of the line in microns not counting magnification factor Copyright 2008 Page 81 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Enter the nominal magnification The magnification is used in printing only where the image appears magnified on
49. e or save an LUT definition Copyright 2008 Page 23 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Menu Commands Most commands are accessible from the menu Some of the more frequently invoked commands also have toolbar buttons and or shortcut key combinations associated with them Those commands that are not accessible from the menu are described in Section 3 Other Commands Relating a toolbar icon to a menu item The toolbar icons denote the button functions available on the various toolbars The same icons appear to the left of the corresponding menu items on the dropdown menus Relating a shortcut key combination to a menu item A short textual description of the shortcut key combination is shown to the right of the corresponding menu item INFINITY ANALYZE has 7 major menus Document Menu Main Frame Menu OLE Container Menu OLE Server Menu Field Group Context Menu Image Window Tab Context Menu Toolbar and Auxiliary Window Context Menu or DE NY Most work will be carried out by commands in the Document Menu which is the default menu when INFINITY ANALYZE starts up The Main Frame Menu will replace the Document Menu whenever there is no image window in the main window The commands contained in this menu are mainly useful in creating a new image window by New for Video Preview or Open or to exit from INFINITY ANALYZE by selecting Exit The Main Frame Menu will not ap
50. e painted with a specific color Vector graphics can have only a limited number of colors and cannot represent natural scenes They are mainly useful in annotating raster images Raster images are resolution dependent that is they represent a fixed number of pixels and can appear jagged and lose detail if they are scaled on screen or if they are printed at a higher resolution than they were created for On the other hand vector graphics are Page 12 dL Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual resolution independent that is they are defined by analytical formulas and so can be scaled arbitrarily to appear crisp and sharp on any output device at any resolution INFINITY ANALYZE works with both raster images and vector graphics The images obtained from cameras are raster images The annotation objects created with INFINITY ANALYZE are vector graphics In a broad sense both raster images and vector graphics are considered digital images or digital pictures Vector graphics are always converted to raster images for purpose of display a process known as digitization INFINITY ANALYZE can also burn vector graphics into raster images This is the same as digitization but is irreversible 2 1 3 The Layers For any image processing program that is more sophisticated than a simple image viewer additional information is stored with the pixel data For example sampling intervals a
51. ed resolution In focusing mode maximal details are shown although the field of view may be partially visible Copyright 2008 Page 53 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Overlay Size Line Pitch Text Size Auto Zoom Line Fitch 1 A relative scale of the pen width for graphical overlay Cent 5 A relative scale of font for textual annotation objects EOE generated during measurement process lf checked both line pitch and text size will be re scaled _ Auto Zoom according to zoom ratio currently used for displaying the image being measured subsequent measurement gt lt Push to close the dialog and discard the changes to _ Cancel overlay size made during the dialog 3 3 8 Centre Use the Centre command to place the centre of the image at the centre of the image window 3 3 9 Corner Use the Corner commands to place a corner of the image at the centre of the image window 3 3 10 Fit to Window Use the Fit to Window command to toggle between browsing and focusing mode of image display Lumenera Copyright 2008 corporation Page 54 d Lumenera INFINITY ANALYZE In browsing mode the full field of view is visible albeit possibly in a reduced resolution In focusing mode maximum image details are shown although the field of view may be reduced to a sub window The image display is in focusing mode whenever scroll bars are provided alon
52. en the image is saved to disk or cached in Field Group will be deleted The process of burning an annotation Copyright 2008 Page 109 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 object includes deleting that temporal layer Annotation layers are topmost layers of an image of INFINITY ANALYZE and may obscure pictorial and overlay layers Annotation is possible only when INFINITY ANALYZE is in annotating mode To enter the annotating mode choose Annotate gt Enable To leave the annotating mode choose that menu item again In annotating mode those measurement commands that involves extended mouse actions will be disabled The hand tool for image pan and scroll and the micrometer calibration are also disabled in annotating mode One or more annotation objects can be selected Click to select an object in the annotating mode Shift Click to select an additional object To select all the annotation objects press Ctrl A To deselect all the annotation objects double click anywhere that is not occupied by an annotation object Selected objects can be moved together To move selected objects drag any one of them Selected objects can also be cloned To clone selected objects click any one of them while pressing down the Ctrl key When a selected object is the only selected object it may be resized To resize an annotation object drag its resizing handles An annotation object can have as many as nine resizing handles loca
53. epresents the amount of gray in proportion to the hue Intensity stands for the relative lightness or darkness of the color It is the energy contained in the color As in the case of the RGB model INFINITY ANALYZE uses a triplet of integers of the range 0 255 to represent a color based on the HSI model However a particular camera supported by INFINITY ANALYZE may implement a camera specific range of values for hue saturation or intensity and may not use integers to represent the value For example some cameras allow the adjustment of saturation in the range 0 2 In this situation it is understood that the real number in the range 0 2 will be scaled to 0 255 and added to the saturation component of pixels in each frame of the video from that camera Luminance is sometimes used in place of intensity in describing a color in the HSI model In camera terminology gain has the greatest similarity to intensity Whenever gain is involved it is multiplicative instead of additive as in the processing of a still image Grayscale and Indexed Color When dealing with a monochrome camera or image the Grayscale model is used to represent the values from colored objects In this case a single integer in the range 0 255 is used to describe the energy content of a color corresponding to the intensity channel of HSI model The other color model that uses a single number to describe the color is the Indexed Color which is based on a palette
54. epts Windows Themes for its auxiliary windows If Auxiliary Window Captions is unchecked as is the default INFINITY ANALYZE will draw the captions of auxiliary windows in its own way which in a sense is more convenient to use However if Auxiliary Window Captions is checked INFINITY ANALYZE will draw the captions of auxiliary windows according to the current Windows Themes This command merely affects the appearance of the auxiliary windows and does not limit their functionalities 3 3 4 Image Window Tabs Copyright 2008 Lumenera corporation Page 48 d Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Use the Image Window Tabs command to toggle the visibility of image window tabs Image windows can have or have not tabs When an image window has a tab the tab can either appear on top or at bottom of the main window Choose Image Window Tabs to hide the tabs if the image windows currently have tabs Choose Image Window Tabs to show the tabs if the image windows currently do not have tabs Equivalent Commands Image Window Tab Context Menu Use Hide Tabs to remove image window tabs currently shown in the main window 3 3 5 Overlay Use the Overlay command to toggle the visibility of measurement and micrometer overlays A successful measurement operation creates graphics overlay on the image This overlay is on a separate layer from pixels The overlay layer can be either shown or hidden Choose Overlay to remo
55. er A short description of the angle will appear in a text box annotation object over the image The auxiliary window Measurement will also have a new entry added The following example illustrates the process Page 104 Copyright 2008 Lumenera INFINITY ANALYZE 3 8 8 Polyline Use the Polyline command to measure the lengths of connected line segments Click to specify endpoints of the connected line segment Right button double click to signify that all endpoints have been added and the Polyline command has been completed The total and average length of the line segments will be calculated The line segments will appear on the overlay layer of the active image A short description of the total and average length of the line segments will appear in a text box annotation object Two entries will be appended to the auxiliary window Measurement One entry is the total length of the line segments The other entry is the average length of the line segments The example below illustrates the process Copyright 2008 Page 105 ZZ Lumenerg Lumenera INFINITY ANALYZE 3 8 9 Circle from 3 Points Use the Circle from 3 Points command to measure the radius of a circle Click to specify three points If these points are not collinear a circle can be unique determined which passes through the three points The circle will appear on the overlay layer of the image A short text describing the quantity of its radius will appear in a
56. f a still image The amount of change for each color channel may be specified by the Increments command 3 6 6 Increments Use the Increments command to set the amount of change made by More and Less commands Copyright 2008 Page 77 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Increments Intensity d Saturation JE Hue d e Stillmage LUve image Intensity The intensity of a pixel The range of applicable gain is in the range 0 255 values is specific to each model Values out of the of camera The increment of gain range will be is proportional to that of the truncated intensity of a still image G aturatior JE The saturation of a The saturation of a camera is in pixel is in the range the range 0 2 The increment of 0 255 Values outof saturation of the camera is the range will be proportional to that of a still truncated image Hue J The hue of a pixel is in The hue of a camera is in the the range 0 255 and range 180 180 The increment is cyclic with a period of hue of the camera is of 256 proportional to that of the hue of a still image Push to dismiss the dialog and discard any changes 3 6 7 Mirror Use the Mirror commands to flip the active image When the active image window is showing the video the command actually flips the live images 3 6 8 Rotate Use the Rotate commands to rotate the active image Page 78
57. g cables or connectors other than those recommended This device complies with part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation European Community CE Statement Products INFINITY 1 2 3 4 5 X Lu1175 Lu1175X Lu1205 Lu1135 Lu1165 Lw1235 Lu1275 Lu1335 Lu1375 and Lw1625 USB Scientific Cameras Directives 2001 59 EC General Product Safety Directive Self declared 89 336 EEC EMC Directive Standards to which conformity is declared EN55024 1998 EN55022 1998 Class B EN61000 3 2 1995 EN61000 3 3 1995 Manufacturer s Name and Address Lumenera Corporation 7 Capella Court Ottawa ON K2E 8A7 Canada Type of Equipment USB 2 0 Scientific Digital Camera First Year of CE Conformity 2003 This is to certify that the Lumenera USB 2 0 Scientific Cameras meet or exceed the standards for CE compliance per the Council Directives noted above All equipment is built at Lumenera Corporation and pertinent testing documentation is available for verification Se d Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Table of Contents TABLE OF ee Ch KC 5 INTRODUCTION ME 9 GETTING START ED EN 10 2 1 MAE ee 10 2 1 1 Goor ole K AE 10 2 1 2 Raster Images and Vecto
58. g the bottom edge and right side of the image Browsing mode is best for finding the right field of view to capture Focusing mode is best for a rapid response to focus adjustments 3 3 11 Zoom Preview Use the Zoom Preview command to toggle the visibility of the zoom preview window Zoom Preview Se Sg 200 Ei i e an cA EE EE aa Oh see DEE sk The zoom preview window is a floating window created automatically by INFINITY ANALYZE upon startup It magnifies a small region of an image The magnification rate is adjustable with the slider at the bottom of the zoom preview window The zoom preview window works on both still image and live image The region to magnify is set by current mouse position Copyright 2008 Page 55 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 3 3 12 Screen Gamma Use the Screen Gamma command to adjust the Gamma of the computer screen Screen Gamma NN E wi OF K Cancel Use the slider to set a new Gamma value I The minimal allowable screen Gamma corresponding to the physical value 0 01 The current screen Gamma scaled up by 100 The maximal allowable screen Gamma corresponding to the physical value 4 Push to dismiss the dialog and keep the screen Gamma setting WO Cancel Push to dismiss the dialog and discard any modification to screen Ae Gamma made during the dialog 3 3 13 Equivalent Commands 1 From Settings File Upon startup INFINIT
59. ge of the full field view will be captured regardless of the preview state If unchecked only the image that is being previewed will be captured This can be a sub region of the full field of view or lower resolution image which INFINITY ANALYZE is using for display purpose Preview captured image If checked a new image window will be created to load a copy of the acquired image Copyright 2008 Lumenera corporation Page 42 d Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Verify file name on capture If checked and the captured image is to store ina file a File Save As dialog will be brought up after each image is acquired The file name contained in the dialog is initially set to the previously specified file name such as e report specimen jpg However it is free to change the file name or instruct INFINITY ANALYZE not to save this image at all l Auto increment file name If checked and the acquired image is to store ina file the file name will be changed by INFINITY ANALYZE after the current image has been saved The new file name will be used for the next acquired image The new file name will have a integer appended to the original text and this integer will be incremented by one each time a new file name is generated by INFINITY ANALYZE 3 1 16 Time Lapse Capture Use the Time Lapse Capture command to acquire a sequence of images at equally Spaced temporal points This com
60. h to add the new record to database Push to exit the dialog and do not modify the database 3 4 2 Load Use the Load command to open the selected record in the database Note that a record in the database must have been selected for this command to work Otherwise a warning message will appear as the following Infinity Analyze ANN Please select a record When a record is successfully loaded a new image window will be created to hold the image data from that record If the image has been measured before measurement information will also be loaded into the auxiliary window Measurement 3 4 3 Delete Use the Delete command to delete the selected records in the database For this command to work at lease one record has to be selected Otherwise the following warning message appears Copyright 2008 Page 59 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Infinity Analyze A Please select a record A deleted recorded cannot be restored 3 4 4 Refresh Use the Refresh command to reset the record list to its default state Upon startup the auxiliary window Database shows the most recently added records up to 100 entries After a query has been made on the database the auxiliary window Database shows the query result Choose Refresh to list the records in order of their creation The Field Group of INFINITY ANALYZE is a buffer of images with a thumbnail displayed for each image F
61. he image will be stored to disk under that file path specified below If Field Group is selected the image will be appended to Field Group if there is still room If Both is selected the image will be stored as a file on disk and also appended to Field Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Group Show the file name to be used when the captured image is to Store on disk A new file name may be entered directly Push to bring out a File Save As dialog to specify the file name to be used when the captured image is to store on disk Capture full field of view If checked the Image of the full field view will be captured regardless of the preview State If unchecked only the image that is being previewed will be captured This can be a sub region of the full field of view or lower resolution image which INFINITY ANALYZE is using for display purpose _ Preview captured image lf checked a new image window will be created to load a copy of the acquired image _ Verify file name on capture lf checked and the acquired image is to store in a file a File Save As dialog will be brought up after each image is acquired The file name contained in the dialog is initially set to the previously specified file name such as e report specimen jpg However it is free to change the file name or instruct INFINITY ANALYZE not to save this image to disk a
62. ication setting as those of the first image in the Field Group 3 5 16 6 Min Use the Min command to create a pixel wise channel wise minimal value image from all images in the Field Group For this command to work all images in Field Group must be identical in the numbers of columns and rows of pixels and the number of color channels and there must be at least two images in the Field Group A new image window will be created to hold the output image The output image has the same numbers of columns and rows of pixels and the same number of color channels The value of each color component channel of each pixel of the output image is set as the least of all corresponding values from all images in the Field Group The output image has the same sampling intervals and magnification setting as those of the first image in the Field Group The example below illustrates the process The first image is pure green simulating a microscope filter The second image is a raw image captured for a specimen The third image is the output of the Min operation with the previous two images as input Copyright 2008 Page 71 DXI Lumenera corporation Lumenera INFINITY ANALYZE 3 5 16 7 Average Use the Average command to create a pixel wise channel wise mean value image from all images in the Field Group For this command to work all images in Field Group must be identical in the numbers of columns and rows of pixels and the number of col
63. ield Group can be a destination of acquired images Images in Field Group can also act as the input to a multi operand image processing operation The Field Group is a complementary means of image organization While an image in an Image Window can be more readily viewed or processed the Field Group provides the concept and infrastructure of an ordered set of images INFINITY ANALYZE has a mechanism for exchanging data between Field Group and Image Window 3 5 1 Open Use the Open command to load images from a Field Group File fov A Field Group File fov is a file format used to hold the content of the Field Group Successfully loaded images from a Field Group file will be appended to the Field Group Note that images in Field Group File are also properly ordered as in the Field Group so the successfully loaded images will be appended to the Field Group in that order Page 60 Copyright 2008 Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual 3 5 2 Save Use the Save command to store the content of Field Group to a Field Group File fov A Field Group File fov is a file format used to hold the content of the Field Group 3 5 3 Import Use the mport command to load image files to Field Group This command is capable of opening multiple image files subject to the conditions that these files reside in the same folder and are in the same format The acceptable file formats are listed below Windows Bitmap
64. ies associated with them An arrow is created moved and resized in the same way as a line The properties of an arrow include besides line and fill properties the shape and size of arrow head and arrow trailer as shown below Copyright 2008 Page 117 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Properties Graphics Line Color me Style _ Gize 3 _ Round Pen Fill DO Traller Style a Size 0 Head Stule Size 2 TO Trailer Style Se Size o ES Head Style ech Size g L Click to pick up a new style of the arrow head gt gt gt gt 8 Page 118 Copyright 2008 Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual The style of the arrow trailer is set in a similar way The shapes of arrow trailer are mirrors of those of arrow head Use the spin button to adjust the size of arrow head or arrow trailer The size is defined as diameter of the bounding circle of the shape measured in pixels 3 9 8 Text Use the Text command to add text box objects Click to add a text box object Unlike other annotation commands the property dialog appear immediately upon the creation of a text box object as illustrated below Properties Text Graphics see here for comments Border E lipse Push to open the standard Windows Font Dialog where a font including its style size and effects m
65. ight Source Temperature Copyright 2008 Page 127 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Capture Frame Averaging S O Options Decimation Destination Where to store the acquired images It can be Field Group disk files or both A new image window may also be created to hold a newly acquired image newly acquired image Capture Full Field INFINITY ANALYZE may be directed to exploit the of View full pixel array of the camera for the image acquisition or just to grab a frame from current video stream which may be sub sampled or only part of the field of view Image copy of the just acquired image Verify File Name Show a File Save As dialog box for each acquired on Capture image that is to be stored to a file This option may be used to prevent overwriting an existing file or to convert the image to another format Auto Increment INFINITY ANALYZE may be directed to name the File Name files automatically to store the acquired images It does so by appending a serial number to the initially specified file name Calibration Sampling interval Information in horizontal Micrometer direction Sampling interval in vertical direction Magnification Page 128 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual 4 1 1 Capture Control Capture Control White Balance Auto Exposure F Area wEB EI Capture
66. ill be based The width and height of the rectangle is the smallest region of interest that is supported by the camera Auto Exposure Push to have exposure time determined and set automatically based on the visible portion of live image The other sub panes will be updated to synchronize with the new exposure time Preview and Capture Push to turn on video preview INFINITY ANALYZE uses an empty image window to display live images At any time at most one of those empty image windows may be streaming video If there are multiple empty image windows and none is being used for streaming video this command randomly picks up one for video preview If a window is already streaming video this command has no effect If there is currently no empty image window this command creates one and displays live images therein mu Video Off Push to turn off video preview if there is currently a window displaying A live images gt Video On Push to acquire an image based on the current camera settings and capture options The acquired image will be automatically calibrated according to system micrometer and present decimation factor Copyright 2008 Page 131 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 4 1 2 Time Lapse INFINITY ANALYZE is able to acquire a sequence of images automatically an operation referred to as time lapse capture Two parameters have to be specified to start a tim
67. images in the Field Group Negative pixel values are truncated to zero 3 5 16 4 Divide Use the Divide command to divide each image in Field Group by the active image For this command to work each image in the Field Group must have the same numbers of columns and rows of pixels and number of color channels as those of the active image The output images will replace those corresponding images in the Field Group If there is a zero pixel in the active image this command fails quietly The output image will be scaled in intensity to make it approximately the same brightness as those of the input images 3 5 16 5 Max Use the Max command to create a pixel wise channel wise maximal value image from all images in the Field Group Page 70 d Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual For this command to work all images in Field Group must be identical in the numbers of columns and rows of pixels and the number of color channels and there must be at least two images in the Field Group A new image window will be created to hold the output image The output image has the same numbers of columns and rows of pixels and the same number of color channels The value of each color component channel of each pixel of the output image is set as the greatest of all corresponding values from all images in the Field Group The output image has the same sampling intervals and magnif
68. l parameters must be within the range 0 1 and x2 1 gt 0 1 C AllChannee Red Green C Blue Apply to The dialog above is displayed when the icon is clicked in the histogram panel Use this panel to select a specific LUT channel or to apply changes to all three channels at the same time It provides an easy option to generate an inverse LUT and can be used to specify the actual coordinates for control points on a relative scale from 0 0 to 1 1 z boer Enter the x y coordinate of the first control point DN 0 433071 j STEIER Enter the x y coordinate of i the second control point 0 645669 Invert the current LUT T Invert apply to Select the active channel or C AllChannele Red Green C Blue all channels o OK Click OK to save the modifications and close the dialog ches Rollback any changes made to the dialog settings and close the dialog Page 22 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual uw Apply bo Camera Apply to Still Image J Reset Define OU Red Green Blue T All Channels Load Save The LUT menu is accessed from the File gt Lookup Table menu Each function represented with an icon beside has been explained in the table above Use the Apply to Still Image option on this menu to apply the LUT to the active image in the ANALYZE working area Use the Load and Save options to restor
69. lance for the camera Equivalent Commands From Camera Control Sub Pane mh Eu 400 Fluorescent int Preset Add White Balance Auto Exposure J AreawB 9 Capture VideoOn C Video Of Push to perform white balance based on a small rectangle within the visible portion of the live image The operation is completed by a click on the live image which specified the center of the rectangle on which the white balance will be based The width and height of the rectangle is the smallest region of interest that is supported by the Ch Page 74 Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual i camera 3 6 3 Interactive Use the nteractive command to adjust the intensity saturation and hue of the active image or the camera Interactive Adjustment Intensity Saturation Indicate the intensity of the image is being adjusted The intensity of a pixel is in the range 0 255 Values out of the range will be truncated Indicate the saturation of the image is being adjusted The saturation of a pixel is in the range 0 255 Values out of the range will be truncated ne Indicate the hue of the image is being adjusted The hue of a pixel is in the range 0 255 and is cyclic with a period of 256 Use the slider to adjust a color component Hue Minimal allowable amount that can be added to a color component Show the current amount that is being
70. layer The graphics generated by measurement operations will also be placed on the overlay layer An annotation object is a temporary layer on top of all permanent layers When annotation objects are being burned into image they will be placed on the pictorial layer Those annotation objects that have not yet been burned when the image is closed will be discarded The graphical output of a measuring operation is placed on overlay layer The textual output of a measuring operation is a text box exactly as that can be created manually with annotation commands except the textual description of measurement result is generated by INFINITY ANALYZE The quantitative output of a measuring operation is one or more new entries in the auxiliary window Measurement Each entry or record has three fields the first field is the name of the command that has generated the entry the second field is the parameter that is being measured and the third field is the numerical result of the measurement operation lf some measurement has been done for an image the measurement results will also be saved when the image is stored to the database When the image is again loaded from the database the previous measurement results will also be loaded 3 8 1 Reset Use the Reset command to clear overlay layer and measurement result of the active image 3 8 2 Abort Use the Abort command to terminate the present measuring operation Equivalent command Right button do
71. lmg w i Vert Sampling A Cancel Magniticatiorn D SE The present horizontal sampling interval in pane anes microns kept in system micrometer ae e The present vertical sampling interval in HI microns kept in system micrometer The present magnification kept in system micrometer The new magnification should be entered here Magrittcation Copyright 2008 Page 79 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Push to dismiss the dialog The newly entered magnification will be accepted and the sampling intervals will be scaled accordingly Push to dismiss the dialog and discard any change to system micrometer Equivalent Commands 1 From Settings File Upon startup INFINITY ANALYZE automatically loads and applies the system micrometer used last time When INFINITY ANALYZE exits it automatically updates the settings file 2 From Capture Control Sub Pane ah ah 400 Fluorescent Ww Preset Add White Balance Auto Exposure eawWwe T Capture VideoOn C Video Of 400 Fluorescent ye Camera presets include micrometer setting If a preset for example 400 X Fluorescent is selected the system micrometer will be replace by the one stored in the preset and all subsequently acquired images will be calibrated according to the system micrometer stored in that preset This helps to avoid measurement errors induced by system error of th
72. mand brings up a dialog box where time interval and total time length can be specified Time Lapse Capture Frequency fa 3 Duration 10 Once the time lapse capture begins the progress will be given upon the completion of each capture as shown below Copyright 2008 Page 43 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Time Lapse Capture Frequency s 3 Duration 3 10 A Cancel 1 of 4 images captured 12 seconds to go Time interval in seconds Frequency s 3 Duration 2 Time duration in seconds Push to start time lapse capture Video preview might be necessary if not to capture full fields of view The dialog is dismissed automatically upon the completion of time lapse capture Push to abort the time lapse _ Cancel capture and dismiss the dialog The progress of the time lapse Equivalent Commands From Time Lapse Sub Pane a Frequency s 3 Duration s 10 1 of 4 mages UK 12s to go Page 44 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Frequency s 3 Time interval in seconds Curation s 10 Time duration in seconds Push to initiate time lapse capture Video preview might be necessary if not to capture full fields of view Push to abort the time lapse Capture The progress of the time lapse capture Both the number of captured images and the e
73. ment of images are specified by Print Image Layout 3 1 10 Auto Exposure Control Use the Auto Exposure Control command to have the application determine an appropriate integration time to deliver an image of acceptable brightness It may be necessary to select this option more than once as the application restricts itself to performing several iterations of exposure time adjustment in an attempt to reach a Suitable brightness Selecting this option a second time may refine the selection of the integration time Equivalent Commands 1 From Capture Control Sub Pane mh Eu 400 Fluorescent We Preset White Balance Auto Exposure A ArawWwe Capture Video On C Video Of i Camera presets include Use the Auto Exposure button Page 32 Copyright 2008 Lumenera corporation Ch Release 4 6 0 exposure setting If a preset for example 400 X Fluorescent is selected the camera will be directed to integrate according to the time value recorded in that preset The position of the Exposure slider in the Camera Control Sub Pane will be updated to synchronize with the current exposure setting 3 1 11 White Balance Lumenera INFINITY ANALYZE User s Manual to initiate an analysis and automatic exposure adjustment INFINITY ANALYZE performs a limited number of exposure adjustments while analyzing the average scene brightness It may be necessary to click the Auto Exposure button a second
74. ment of the built in database and the interaction of the active image and the database may be carried out from the auxiliary window Database Database D Gel P searchin WA Caption Description Inclusion 2006 04 25 16 13 35 John Mon metal inclusion in Tissue 2006 04 22 18 01 25 Jack The haze illustrates spherical aberration Ik cannot be Bone 2006 04 22 16 35 42 Jack Radiograph processed 45 pio 4 1 7 Store Push E to save the active image and its measurement result to the built in database Copyright 2008 Page 141 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Database Caption Bone Date 4 22 2006 Author Jack Description Radiograph processed as photomicrograph Laption Specify a caption for the record to create Must be a single line of text The title of the active image is initially displayed but it is free to specify a different one The caption should be unique within the database Otherwise a warning message appears as shown below Answer Yes to replace the existing record with the same caption Answer No to abort the storing operation Bone Infinity Analyze ie ei Database already contains a record captioned Bonge Would you like to replace the record Date Specify the date of creation of the record i The date initially shown is that of the o i computer Click to pick up another date as illustrated below Page 142 Copyright 2008 Lumenera I
75. mited to 16 columns of pixels A new image window will be created to hold the output panorama image The output image copies the sampling intervals and magnification setting from the first input image from Field Group Due to the overlapping of the input images the number of rows of the output image would be less than those of input images summed Due to possible horizontal shift of the input image the number of columns of the output image might also be less than that of an input image The cropping is automatic and transparent to users The example below illustrates the process The three images on the left are stitched to create the image on the right Page 66 Copyright 2008 Lumenera INFINITY ANALYZE 3 5 15 Multi Focus Composition Use the Multi Focus Composition command to create a best focused image from partially focused images in Field Group The partially focused images at least two must have been cached in Field Group All images in Field Group will contribute to the focus of the output image These input images must be identical in the numbers of columns and rows of pixels and the number of color channels For the multi focus composition to work the active image must also have the same number of columns and rows of pixels and the same number of color channels When the computation starts the active image will be replaced by the first image in the field group As the following images in field group are aligned and fu
76. n the menu is docked it can be docked to any of the four sides of the main window Drag the grip or caption of the menu to adjust its position or to dock it to a particular side of the main window The grip of the menu is the dot matrix at the left or top of the menu in docked state 2 2 2 Toolbar INFINITY ANALYZE has four toolbars Each toolbar can be either shown or hidden When a toolbar is shown it can be either docked or floating Double click its grip or caption to toggle between the two states When a toolbar is docked it can be docked to any of the four sides of the main window Drag the grip or caption of a toolbar to adjust its position or to dock it to a particular side of the main window The grip of a toolbar is the dot matrix at the left or top of the toolbar in docked state 2 2 3 Status Bar Copyright 2008 Page 15 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 The status bar is located at the bottom of the main window Depending on whether a still image or the video is being displayed in the active image window the status bar provides different information The status bar is divided into thirteen panes from left to right The information displayed in each pane is as follows Still Image Leftmost 2 Mouse position in horizontal direction in The horizontal offset of the iw pixels relative to the top left corner of the region of interest on the active image camera 3 Mouse position in vertical di
77. nd dragged to an external spreadsheet program for further processing With the external program already running drag the highlighted measurement data over the program icon in the task tray at the bottom of the screen This brings the spreadsheet program to the foreground where the measurement data can be dragged to the desired location Release the mouse button to paste the selected data into the spreadsheet at the mouse pointer location Histogram Histogram A histogram is a graphic representation of the number of pixels at each brightness level in an image INFINITY ANALYZE plots histograms for all three channels of the active image based on RGB color model In the black square box the x axis represents the color value from darkest 0 at the far left to brightest 255 at the far right the y axis Copyright 2008 Page 19 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 represents the frequency Le the total number of pixels with that value the origin of the histogram is the bottom left corner of the black box Note that histograms of red green and blue channels are shown in the same coordination system as red green and blue bars and so they may overlap The following table helps to tell the overlapping range of ee i _ the histograms Green and Blue NN eg The histogram can be shown for both still and live images For a still image its histogram is updated whenever the im
78. nd magnification settings are also recorded in an image within INFINITY ANALYZE In fact even the pixels have to be organized into layers Each layer is either a raster image or a vector graphics object Layers may be combined in various ways to produce specific presentations of an image within INFINITY ANALYZE The presentation itself is a simple raster image An image in INFINITY ANALYZE has three layers The bottommost layer is the pixel array also called the pictorial layer The topmost layer is the overlay where the graphics generated by measurement operations and micrometer are placed In between is the mask layer This layer is used by advanced editions of INFINITY ANALYZE to hold segmentation results In displaying an image its pictorial layer is drawn first The layers above the pictorial layer are drawn later and may obscure the pictorial layer When INFINITY ANALYZE is running there can be temporary layers hosting annotation objects Each annotation object occupies a temporary layer by itself The temporary layers are above any other types of layers Annotation objects may be burned into the image where the pictorial layer is modified according to the vector graphics of the temporary layers Unburned annotation objects i e temporary layers are discarded when the image is closed 2 1 4 The Active Image With INFINITY ANALYZE many images can be open simultaneously However only one of them may receive input focus This image is
79. ns for burning micrometer 3 7 1 Flatfield Correction Use the Flatfield Correction command to filter the active image to make appear as if having been evenly illuminated The example below illustrates the process Copyright 2008 Page 83 Lumenera INFINITY ANALYZE 3 7 2 Flatfield Correction Options Use the Flatfield Correction Options command to customize the filter employed in Flatfield Correction Page 84 Copyright 2008 Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual The support of the Gaussian Homogenous Radius 2 filter that will be used to detect illumination distribution Intuitively it is the radius in pixels of the biggest circle within which illumination remains even The default value 31 should work well in most cases A greater value may be specified if the illumination is flatter A smaller value may be specified if the illumination varies more abruptly Push to dismiss the dialog wv DK and update the parameter for use with Flatfield Correction Push to dismiss the dialog a Cancel and keep the original parameter of Flatfield Correction 3 7 3 Denoise Use the Denoise command to suppress noise in the active image The example below illustrates the process Copyright 2008 Page 85 Lumenera INFINITY ANALYZE 3 7 4 Remove Bad Pixels Use the Remove Bad Pixels command to detect and make up malfunctioning pixels The example below illustrates the process
80. nt 4 1 9 Delete Push 3 to delete the selected records in the database Note for this command to work at least one record has to be selected Otherwise the following warning message will appear Infinity Analyze A Please select a record A deleted record cannot be restored Page 144 Copyright 2008 Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual 4 1 10 Refresh Push S to reset the record list to its default state Upon startup the auxiliary window Database shows the most recently added records up to 100 entries After a query has been made on the database the auxiliary window Database shows the resulted record list Choose Refresh to list the records in the order of their creation 4 1 11 Query Push Lok to search for particular records in the database Before the search criteria of search have to be established Query by Date Click SEA and select Date The toolbar of the auxiliary window 4232006 5 41252006 Ze Database shows the two date control 23 where the time interval during which the search will be done may be specified Use the spin buttons to 33 adjust starting and ending date Push Lt to start searching The search result will be given in the record list Query by Author Search in 8 Click and select Author The toolbar of the auxiliary window Database shows the edit control John where the name of the author on A which the search will be d
81. ntations and light source temperature For more details please refer to respective commands that set these parameters The capture options include frame averaging decimation where to store the captured images and so on For more details please refer to the Capture Options command Equivalent Commands 1 Shortcut Double click the empty image window that is displaying video to initiate an image capture The resulting camera image will be stored directly to file into the field group or both places based on the settings selected If the option to preview the captured image is selected then the resulting camera image will be opened in a new window 2 From Capture Control Sub Pane Copyright 2008 Page 39 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 mh Eu 400 Fluorescent Se Preset White Balance Auto Exposure A ArawWwe 26 Capture VideoOn 0 Video Of F l Capture Push to acquire an image from the camera based on the present wem P camera settings and capture options Equivalent Commands 1 From Settings File Upon startup INFINITY ANALYZE automatically loads and applies the capture options used last time The capture options and many other options and settings are specific to each camera and are stored in a file that is uniquely identifiable by the serial number of the camera When INFINITY ANALYZE exits it automatically updates the file 2 From Capture Control
82. of 256 colors INFINITY ANALYZE does not attempt to interpret the color in an image of indexed color Whenever it reads a file of indexed color image it converts the image to grayscale by simply mapping the indices to the intensities of a grayscale image Color Components and Channels In the RGB model each of the red blue or green values is referred to as a component or channel of the color Color component and color channel terms that are used interchangeably in this document The same is true for the HSI model and the Grayscale model A color described by the HSI model has three components or channels namely the hue channel the saturation channel and the intensity or luminance channel A color described by Grayscale model has only one channel namely the intensity channel Copyright 2008 Page 11 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 The triplet used by the RGB and the HSI models and the single number used by Grayscale model are called the color value Color Model Conversions RGB and HSI are equivalent means to describe colors The main reason to use multiple color models is to facilitate the understanding of image processing of INFINITY ANALYZE The internal conversion between the two models is handled implicitly and needs no user input RGB and HSI may also be used to represent a monochrome image as Grayscale model does In this situation the three channels of RGB are set equ
83. of the paper in millimeters The offsets are specified in Displacement for CAP mm e CFP Under this mode the active image would be printed if it is not empty The image is automatically scaled to fill the width or height of the paper depending on aspect ratios of the image and paper The aspect ratio of the image is kept constant If the image could not occupy the whole printable area of the paper it Copyright 2008 Page 29 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 will be placed to the top side as far as possible but centered horizontally on the paper Zoom for AAA and CAP This section specifies a zoom ratio of the images for use with printing under AAA and CAP modes Displacement for CAP mm This section specifies offsets of the image for use with printing under CAP mode The Origins of the image and paper are their top left corners Horizontal displacement is defined as the distance between the leftmost column of the image and the left side of the paper s rectangular printable area Vertical displacement is defined as the distance between topmost row of the image and the top of the paper s rectangular printable area The distance is measured in millimeters The Print Image Layout command is used to configure the printing but it does not initiate a printing process 3 1 8 Print Preview Use the Print Preview command to simulate image printing on computer screen In this
84. og and to apply the settings when INFINITY ANALYZE runs again and not to change any setting 3 11 2 Database Settings Use the Database Settings command to choose an alternate location and file for the INFINITY ANALYZE database record and the storage location of the the performance of INFINITY ANALYZE Page 124 d Lu menera Copyright 2008 corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual settings for Database 9 Use local default Gi Use other MS Access file Database Mame T DatabasesMy0B mdb J Image File Folder E HESE dg select this option to use the default database stored in the same location where INFINITY ANALYZE is installed EH TE Select this option to specify an alternate location for the database file and image storage SE Identify the location and filename of the remote database It can be ona network drive or in another local folder as desired Hint Copy the default database file from the INFINITY ANALYZE folder location to this new location prior to invoking this feature Soe location where database images are to be stored Sie ee location EE and close the dialog Copyright 2008 Page 125 GZ Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 3 11 3 Manual Use the Manual command to open this User s Guide from within INFINITY ANALYZE 3 11 4 Online Help Use the Online Help to connect to the Lumenera websi
85. olors Curent Again click a hexagon to pick up its color as the new overly color The present overlay color is shown in a box at the bottom right corner of the dialog box The present selection is shown in the box above the present overly color box Push OK to dismiss the dialog and set the new color as overlay color Push Cancel to dismiss the dialog with overlay color remaining the original If it becomes necessary to create a unique color click Custom to switch to the other tab of this two tab dialog box as shown below Colors Standard Custom Colors 1 Hue A3 Sa Bed 255 amp Le Sat 255 Green 255 A Li Lum 125 Blue 0 Curent Copyright 2008 Page 51 GZ Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Click or drag to adjust hue and saturation of the currently selected color Click or drag to adjust luminance intensity of the currently selected color show the hue of the currently selected color The hue value can also be entered directly or adjusted with the spin buttons The hue is in the range 0 255 app L Show the saturation of the I currently selected color The saturation value can also be entered directly or adjusted with the spin buttons The saturation is in the range 0 255 jag L Show the luminance or SI intensity of the currently selected color The luminance or intensity can also be entered directly or
86. one may be specified Push 4 to start searching The search result will be given in the record list Query by Description Click SEA and select Description The toolbar of the auxiliary window Database shows the edit control ge where one or more words A on which the search will be done may be specified Push Lt to start searching The search result will be given in the record list Copyright 2008 Page 145 DXI Lumenera corporation Microscope World 6122 Innovation Way Carlsbad CA 92009 www MicroscopeWorld com PHONE 760 438 0528 FAX 760 438 0553 TOLL FREE USA 800 942 0528 info microscopeworld com
87. or channels and there must be at least two images in the Field Group A new image window will be created to hold the output image The output image has the same numbers of columns and rows of pixels and the same number of color channels The value of each color component channel of each pixel of the output image is set as the mean of all corresponding values from all images in the Field Group The output image has the same sampling intervals and magnification setting as those of the first image in the Field Group 3 5 17 Color Composition For a description of the Color Composition function see the technical Note in the INFINITY ANALYZE documentation folder typically located here C Program Files Lumenera Corporation INFINITY Software INFINITY ANALY ZE Documentation Page 72 Copyright 2008 Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual 3 6 1 Image Gamma Use the mage Gamma command to adjust the Gamma of the active image or the camera Image Gamma 0K K Cancel _ Use the slider to specify a factor to be multiplied to the original Gamma of the active image Di The minimal allowable multiplier Show the current factor being multiplied to the original Gamma of the active image A value can also be entered directly The maximal allowable multiplier Push to dismiss the dialog and finalize the modification to the Gamma of the active image Push to dismiss the dialog and discard any mo
88. orporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 333 AANA VVINOOW Captions E 48 s34 HMO WNdoW FS isaac sae ee 48 E OVON genera ane er ere re 49 iS ro mo OWEN e E 49 3 3 7 Overlay Size 53 3 3 8 Genre 54 3 3 9 EE 54 3 3 10 Fitto tee e 54 3 3 11 LOOM Hrevieuw 2 ccccccccccccecececececececececececececesesesececeseseseseseseneseseseseneaeaenenees 55 3 3 12 Screen E ge e E EEN 56 3 3 13 Equivalent Commande 56 3 4 DATABASE MENU coctcacacestasatcectasctcnutanctaseeaceecsntenesanseenceaneteececeetenatandtecnseneanes 5 3 4 1 te 57 3 4 2 LOJO GE 59 3 4 3 EE 59 3 4 4 PTS EE 60 39 SET ENEE 60 Sy OOT EE 60 3 5 2 EE 61 OD Os 61 24 Export 62 oS Go APD O EEN 62 3 5 6 ae EEN 62 397 MOVO UD ne ee 63 3 5 8 MOVE DOWN EN 63 3 5 9 CO coi se ese see sn 63 iS om O CON E 64 3 5 11 SNOW PICVIOUS EE 64 3 5 12 Show NOX oie e ieee cc cece cece ececececeecececececneneneaeseceeneneneaeseseceeeaeaeseaesesesnenentaees 64 3 5 13 Panorama Eeer 65 3 5 14 Panorama PoOrTall ee re ee ere ner entre ete etree tan 66 35 19 MUIU FOCUS GOMPOSINOR siiccicisrieresrieeamavanimimrienariainmaieene 67 3 5 16 Math EE 69 BOF LOO C OPOS ON eaa A EE E A E E 72 3 6 THE ADJUST MENU 13 3017 TIGA ce ee 73 3 6 2 Area Based White Galance 74 3 6 3 ere CIC etter iri e ela celta ele ciel cle delet oldest lease ets easels 75 3 6 4 ale EE 77 3 6 5 E L 3 6 6 Increments E L SO MNO EE 78 3 6 8 Eet 78 BOF ACAI C QUOI serait onset ce manecn T autedee ghee 79
89. ose gray Click More Colors to bring up the standard Windows Color Picker Dialog where a new color may be defined lf checked the Color property will be ignored and the interior of the rectangle will not be painted to allow the otherwise obscured objects or pixels to be visible Transparent 3 9 4 Round Rectangle Use the Round Rectangle command to add round rectangle objects A round rectangle is similar to a rectangle except that its corners are made up of arcs The creation moving resizing and editing of a round rectangle is similar to those of a rectangle except for the following A round rectangle has nine resizing handles The handle within the round rectangle as shown below is used to adjust the roundness of the corners Copyright 2008 Page 115 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 A round rectangle has limited line properties The line styles other than the solid cannot be specified for a round rectangle 3 9 5 Ellipse Use the Ellipse command to add ellipse objects An ellipse is fully specified by its minimum bounding rectangle The creation of an ellipse object is identical to that of its minimum bounding rectangle as illustrated below although the rectangle is not visible when the ellipse is not selected a ee An ellipse is moved resized in the same way as a rectangle object The properties of an elliose are identical to those of a roun
90. ould be incorporated in image formation Copyright 2008 Lumenera corporation Page 138 d Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual 4 1 6 Camera Control Extended The saturation hue brightness and contrast of the camera may be controlled from this sub pane Note that adjusting saturation of a camera means to modify the saturation of each pixel in each frame of the video stream from that camera by the specified amount Note also that the range of saturation of a camera must be scaled to match that of a pixel which is always 0 255 The same is true for hue Brightness and contrast are purely camera specific All these parameters of the camera are adjusted independently of the others that is each type of adjustment is separately applied to the raw video frames of the camera a Saturation 0 Hue 1850 10 180 Brightness 100 19 100 Contrast 0 A 100 Saturation Use the slider to adjust the saturation of the camera The minimal saturation allowable for the camera If set to 0 a color camera appears to be delivering monochrome images This is equivalent to subtracting 256 Copyright 2008 Page 139 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 pixel in a still image Show the current saturation of the camera A new value may also be entered here The default setting is 1 where the saturation of the raw image is not modified
91. ove or obscure any trademark or copyright notices Limited Warranty Hardware and Software ANY USE OF THE SOFTWARE OR HARDWARE IS AT YOUR OWN RISK THE SOFTWARE IS PROVIDED FOR USE ONLY WITH LUMENERA S HARDWARE AND OTHER RELATED SOFTWARE THE SOFTWARE IS PROVIDED FOR USE AS IS WITHOUT WARRANTY OF ANY KIND TO THE MAXIMUM EXTENT PERMITTED BY LAW LUMENERA DISCLAIMS ALL WARRANTIES OF ANY KIND EITHER EXPRESS OR IMPLIED INCLUDING WITHOUT LIMITATION IMPLIED WARRANTIES OR CONDITIONS OF MERCHANTABILITY QUALITY AND FITNESS FOR A PARTICULAR PURPOSE LUMENERA IS NOT OBLIGATED TO PROVIDE ANY UPDATES OR UPGRADES TO THE SOFTWARE OR ANY RELATED HARDWARE Limited Liability Hardware and Software In no event shall Lumenera or its Licensor s be liable for any damages whatsoever including without limitation incidental direct indirect special or consequential damages damages for loss of business profits business interruption loss of business information or other pecuniary loss arising out of the use or inability to use this Software or related Hardware including but not limited to any of Lumenera s family of products Product Warranty Lumenera Corporation warrants to the original purchaser that our cameras are guaranteed to be free from manufacturing defects for a period of one 1 year from the original date of purchase Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual
92. pear when there is an image window in the main window Copyright 2008 Lumenera corporation Page 24 d Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual The OLE Container Menu will be called up only in the image annotation process when an external object is inserted and the server of this object is activated In this situation The OLE Container Menu will merge with the object s server menu The OLE Server Menu will be invoked when a native image object of INFINITY ANALYZE is embedded into a document of another container program and is being edited INFINITY ANALYZE will load the embedded image into its own image window When this image is the active image the OLE Server Menu will be used The Field Group Context Menu may be brought up by right click anywhere within Field Group The Image Window Tab Context Menu may be brought up by a right click anywhere within the tab area of image windows The Toolbar and Auxiliary Window Context Menu may be brought up by a right click in any area of the main window that is not occupied by other menus toolbars auxiliary windows or image windows In the following paragraphs only the Document Menu is described The other menus often duplicate part of the Document Menu to provide a level of convenience Where they are unique they typically represent simple commands that are self explanatory The File menu commands are used to open save print and capture images Some of
93. r GraphiCs ccccscccccssseccnsesccnensccsensensensensnees 12 2 1 3 The LAY OMS ec cceccsscccseccnsecnsusccuscnsusnausecusensusnausecusensusnsusecusensusssuseseeenenssaes 13 2 1 4 TINS PACT VC eg 13 2 1 5 The Empty Image WINGOW vivo scceseertenatertwestcuneeuatnwnereniunvereciualnecenueenuais 15 2 2 WOpkKGPACE 15 2 2 1 EE 15 2 2 2 TOODA E 15 2 2 3 IS EE 15 2 2 4 Te Ate 16 MENU COMMANDS E 24 3 1 THE FILE MENU 25 3 1 1 New for Video Preview 2 cccccccececececececececececececececesececececeseseseseneneneneness 25 SA a O E 26 3 1 3 We 27 3 1 4 ee ee a ea ee EN 27 SEET EE 27 Ki FI OUD ee ee ee cere ee eee E re eres ee eee eee 28 Eege 28 3 1 8 get 30 3 1 9 POE PE 32 3 110 AU EEN 32 3 1 11 White Balance 0c cc cccccccccccececececececececececececececeseseceseseceseseseseseseseseseneaees 33 3 1 12 Manual White DGalaonce 34 KKK GC me ee 34 3 1 14 Toggle PLeViOW cccccccncecccescnsecccusccescneuensusessuensusseusesesensusssusessessaussaaeess 38 E EE EE 39 Sa GE Ren tc 8 fc Ee E 43 3 1 17 Lookup TOI E 45 K CC EE 46 3 2 THE EDIT MENU 2 eee ee ec ec ece ec ececeeeeeeeeeececeeeeeeaeseeenececeeaeeeseneeeeevanaeneneneeeneneees 47 3 2 1 nde 47 EE EE 47 Bee COV O C0080 caer ee ee eee 47 3 3 HE VIEW MENU ssscsccvicewcoraies es cnvicnoisecbatvierobessiesebat ickwhossiobeieviboradesvaatehondleseleurias 47 oo Toolbars and Auxiliary Wndouws 47 3 3 2 Slalus Bal ee 48 Copyright 2008 Page 5 DXI Lumenera c
94. rection in The vertical offset of the pixels relative to the top left corner of the region of interest on the active image camera The red component of the pixel at the mouse position oixels The green component of the pixel at the mouse position pixels mouse position the pixel array of the camera mouse position pixel array of the camera E the mouse position camera JL ss E ME mouse position milliseconds of the camera position camera the system micrometer of view In microns Rightmost of view in microns 2 2 4 Auxiliary Window INFINITY ANALYZE has five auxiliary windows Control Panel Help Desk Database Measurement and Histogram Each of these auxiliary windows can be either visible or hidden When an auxiliary window except Control Panel is shown it can be either docked or floating Double click the grip or caption of an auxiliary window to toggle Page 16 S Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual between the two states When an auxiliary window is docked it can be docked to any of the four sides of the main window Drag its grip or caption to adjust the position of an auxiliary window or to dock it to a particular side of the main window The grip of an auxiliary window is the dot matrix at the left or top of the caption bar of the window in docked state All the auxiliary windows can be resized in the same way as an ordinary
95. representation of all the annotation objects on the pictorial layer and delete all the annotation objects Annotation objects are temporary and cannot be saved into a file Page 122 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE 3 10 1 Cascade Use the Cascade command to make all image windows the same size and stacked from top left to bottom right of the main window 3 10 2 Tile Horizontally Use the Tile Horizontally command to make all image windows aligned edge to edge horizontally and the same size if possible 3 10 3 Tile Vertically Use the Tile Vertically command to make all image windows aligned edge to edge vertically and the same size if possible 3 10 4 Arrange Icon Use the Arrange Icon command to align the icons of image windows at the bottom of the main window Settings Settings for Database Manual Online Help About INFINITY AMALYZE Copyright 2008 Page 123 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 3 11 1 Settings Use the Settings command to tune the performance of INFINITY ANALYZE Settings Humber of Fields wi Preallocated Memory A Cancel Number of Undo Steps Screen Gamma 100 NOTE Effective After Restart This Program i The number of thumbnails in Number of Fields J Field Group The size of the memory in Field Group The maximum length of image screen in hundredth vo DK Push to dismiss the dial
96. reviously saved in the native format ef this command will simply update the corresponding file If the image has never been saved in the native format this command will act as File gt Save As 3 1 5 Save As Use the Save As command to store the active image onto disk with a new file name or in a new format The native image format sif and Metallograph format meg retains both pictorial and metrical information and are the best for archival purpose The other image formats keep pictorial information only The supported image formats are listed below Copyright 2008 Page 27 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Format File Remark Extension Containing complete information Bitmap siz Writing in Windows Bitmap format Group compressing mere m sson Format Portable Bitmap Writing in binary format Portable Gray Map Writing in binary format Portable Network PNG Graphics Portable Pixel Map Writing in binary format O Targa File Metallograph Containing calibration and magnification information 3 1 6 Print Setup Use the Print Setup command to select a printer and set its properties for use with image hardcopy output 3 1 7 Print Image Layout Use the Print Image Layout command to specify whether all images or only the active image should be printed and the way each image is to be aligned and scaled This command brings up the dialog shown below
97. rmediate image that is the input to subsequent post capture processing 4 and 8 are similarly defined Frame averaging reduces noise If n frames are averaged the energy of the noise will be 1 n of its original level Frame averaging is performed only when capturing full field Copyright 2008 Page 135 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 pf fviewe Subsampling Gi O2 02 4 For either the INFINITYX 21 or INFINITYX 32 camera models the Subsampling option is replaced by the following Fisel Shutting Gi 02 03 Q4 To File Gei Field Group C Both Page 136 Ch Subsample the output image lf 7 is selected no sub sampling will be done If 2 is selected the number of columns and number of rows of the image will be reduced by a factor of two by merging every two consecutive columns and rows 3 and 4 are similarly defined Subsampling reduces the noise level much like averaging The way INFINITY ANALYZE performs subsampling also helps to avoid aliasing Subsampling is performed only when capturing full field of view Pixel Shift Cameras Instead of decimating pixels pixel shift cameras such as Infinity X are decimating the Original sampling interval which actually leads to an increase of pixel numbers in each row and column by the decimation factor Select a destination of the acquired images If File is selected t
98. rs and Auxiliary Windows Use the Toolbars and Auxiliary Windows commands to toggle the visibility of each toolbar or auxiliary window Auxiliary Window Copyright 2008 Page 47 pLu menera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Standard Control Panel Image Processing Help Desk Image Measurement Image Annotation pf Histogram S O A toolbar can be either visible or hidden When it is visible it can be either docked or floating The menu can also be docked or floating although it cannot be hidden Drag its grip to adjust the position of a toolbar or the menu Double click on its grip to toggle the dock state of a toolbar or the menu Control Panel can be visible or hidden However it is always docked to the left side of the main window Control Panel can be resized but cannot be floating or docked to any other side of the main window The other auxiliary windows can be either visible or hidden When they are visible they can be either docked or floating Drag their captions to adjust their positions Double click on their captions to toggle their dock states 3 3 2 Status Bar Use the Status Bar command to toggle the visibility of the status bar 3 3 3 Auxiliary Window Captions Use the Auxiliary Window Captions command to turn on or off the Windows Themes on all the auxiliary windows INFINITY ANALYZE is able to draw the captions of the auxiliary windows in its own way but also acc
99. s and mouse operations This section lays a background for image capture and analysis with INFINITY ANALYZE 2 1 1 Color Models Digital images record colors by numbers Different color models are used to interpret the digital values representing pixel intensity readings INFINITY ANALYZE uses a number of color models for image acquisition display processing and printing In most cases the underlying color model is transparent to users Where they are explicit an understanding of RGB and HSI models suffices for the purpose of working with INFINITY ANALYZE RGB A large portion of the visible spectrum can be represented by mixing three basic components of colored light in various proportions and intensities The primary components used are the colors red green and blue RGB Computer screens use an RGB model to display graphics and images Most 8 bit image processing programs including INFINITY ANALYZE employ a triplet of integers in the range 0 255 to represent a color being described by the RGB model Page 10 Copyright 2008 Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual HSI Human beings understand colors by their three fundamental characteristics hue saturation and intensity HSI Hue is the wavelength or frequency of light reflected from or transmitted through an object Most often hue is identified by the name of the color such as orange or purple Saturation is the strength or purity of the color and r
100. se 4 6 0 Histogram Histogram 3 7 11 Amplitude Depletion Use the Amplitude Depletion command to extract the texture of the active image The example below illustrates the process Copyright 2008 Lumenera corporation Page 94 d Lumenera INFINITY ANALYZE 3 7 12 Darkfield Simulation Use the Darkfield Simulation command to generate a darkfield representation of a brightfield image The example below illustrates the process Copyright 2008 Page 95 Lumenera INFINITY ANALYZE 3 7 13 Photometric Transform Use the Photometric Transform commands to generate a grayscale or inverted representation of the active image The Monochrome command removes color information from the active image and leaves only intensity information as shown below Page 96 S Lu menera Copyright 2008 corporation Lumenera INFINITY ANALYZE The Negative command reverses the lookup table of the active image to produce an image that is complementary in color with the original image as shown below Copyright 2008 Page 97 Lumenera INFINITY ANALYZE 3 7 14 Spherical Aberration Correction Use the Spherical Aberration Correction to reduce the haze over the active image due to spherical aberration of the optical system The example below illustrates the process Page 98 Copyright 2008 Lumenera INFINITY ANALYZE 3 7 15 Spherical Aberration Correction Options Use the Spherical
101. se 4 6 0 User s Manual For this command to work the selected image in the Field Group must have the same numbers of columns and rows of pixels and number of color channels as the active image otherwise the command fails quietly but the internal index will be incremented so next time the command is executed it can find the right image to display The Show Next command replaces the image in the active image window by the selected image in the Field Group Note that only pictorial data are replaced and the other layers such as graphical overly are kept intact This facilitates the comparing of multiple images 3 5 13 Panorama Landscape Use the Panorama Landscape command to stitch a row of images The images to stitch should have been stored in Field Group and ordered correctly All images in Field Group participate in the operation These images must be identical in the numbers of columns and rows of pixels and the number of color channels The first image in Field Group should be the leftmost image of the row of images to be aligned The last image in Field Group should be the rightmost image of the row of images to be aligned There must be at least two valid images in Field Group In addition any two adjacent images must have at least 20 non overlapping areas and at least 32 overlapping columns of pixels The images are allowed to shift vertically but the shift has to be limited to 16 rows of pixels A new image window will be create
102. sed with the previous ones the active image will be replaced by the current state of the focus combination There are two concatenated stages of multi focus composition The first stage is the registration of the two adjacent images and the second stage is the fusion of two images The registration is the process to correct the image displacement It is used only when imaging with stereomicroscopes The fusion is the heart of multi focus composition and is common to all types of microscopy The Multi Focus Composition command brings up the following dialog box For images acquired from compound microscopes with vertical objectives simple press Start Page 67 Copyright 2008 GZ Lumenera Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 button To fuse images acquired from stereomicroscopes a single parameter Maximum Step Displacement MSD needs to be set before pressing Start button This parameter should be set as an upper bound of the translation in pixels between any two adjacent images Note it is not absolutely necessary to specify a precise value for the MSD as it is used only as a hint to the computation process for efficiency purpose Multi Focus Composition Maxinum Step Displacement Index i After the Start button is pressed the computation starts The intermediate results are given in both the dialog box and the active image as shown below First of all the Start but
103. setting and many other settings are specific to each camera and are stored in a file that is uniquely identifiable by the serial number of the camera When INFINITY ANALYZE exits it automatically updates the settings file 2 From Capture Control Sub Pane amt ah 400 Fluorescent hd Preset Add White Balance Auto Exposure A ArawWwe 26 Capture VideoOn C Video Of 400 Fluorescent vw Camera presets include light source setting If a preset for example 400 X Fluorescent is selected the camera will be directed to modify the directly captured image to add a tint typical of objects illuminated with a light source of a temperature recorded in that preset The Light Source Sub Pane will be updated to synchronize with the current settings 3 From Light Source Sub Pane Page 37 Copyright 2008 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 a Fluorescent O Daylight Incandescent O senon Halogen Other Select to indicate to INFINITY ANALYZE that the light source has an interrupted spectrum Daylight Select to indicate to INFINITY ANALYZE to add more red Select to indicate to INFINITY ANALYZE to add even more red Select to indicate to INFINITY ANALYZE to add more blue Select to indicate to INFINITY ANALYZE to add more red and blue Other Select to indicate to INFINITY ANALYZE no color temperature should be incorporated in
104. soft Office Excel Worksheet Microsoft PowerPoint Presentation Microsoft PowerPoint Slide Microsoft Word Document Microsoft Word Picture _ Display As Icon Result Inserte a new Adobe Acrobat Document object Ki into Your document 3 9 10 Select Use the Select command to select one or more annotation objects Click to select an annotation object Shift Click to select additional objects It is also possible in this mode to draw a rectangle to select all objects covered by the rectangle Click anywhere within the active image window that is not occupied by any annotation object to deselect all objects 3 9 11 Select All Use the Select All command to select all annotation objects 3 9 12 Delete Use the Delete command to delete selected annotation objects 3 9 13 Properties Use the Properties command to edit the properties of a selected object Copyright 2008 Page 121 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 3 9 14 Move to Uppermost Use the Move to Uppermost command to make the selected object the topmost layer 3 9 15 Move to Lowermost Use the Move to Lowermost command to make the selected object the bottommost layer 3 9 16 Move Upper Use the Move Upper command to move the selected object one layer above 3 9 17 Move Lower Use the Move Lower command to move the selected object one layer down 3 9 18 Burn into Image Use the Burn into Image command to create a pixel
105. stimated time in seconds needed to complete the task are given 1 of 4 images UK 14s to go Time lapse capture depends on the current camera settings and capture options When the Time Lapse Capture command is in execution these settings and options are locked until all requested images have been acquired However it is possible to change camera settings and capture options during a time lapse capture operation initiated from Time Lapse Sub Pane It is even possible to capture separate or additional images or to carry out any other tasks which INFINITY ANALYZE is able to do because the time lapse capture is in background processing 3 1 17 Lookup Table The Lookup Table function provides access to the dynamic lookup table controls The functionality of the controls is explained in detail in section 2 2 4 on the histogram pane Copyright 2008 Page 45 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 We Apply bo Camera Apply to Still Image cir Reset Define nl Red Green Blue ee ll Channels Reset the active LUT channel pen te LUT eding cao Me Make the Red channel active Make the Green channel active Mm Make the Blue channel active ee Activate all three channels Enable Disable the LUT on the Live Image me Ei 3 1 18 Exit Use the Exit command to close INFINITY ANALYZE This command prompts to save modified documents INFINITY ANALYZE consi
106. t 2008 Page 3 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Federal Communications Commission FCC Statement This equipment has been tested and found to comply with the limits of a Class B digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a residential environment This equipment generates uses and radiates radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference However there is no guarantee that interference will not occur If this equipment does cause interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to correct the interference by one of the following measures e Reorient or relocate the receiving antenna e Increase separation between the equipment and receiver e Connect the equipment into an outlet on a circuit different from which the receiver is connected e Consult the dealer or an experienced radio TV technician Any changes or modification to said product not expressly approved by Lumenera could void the user s authority to operate this device Shielded and grounded cables and connectors must be used to meet FCC emission limits Lumenera takes no responsibility for any radio or television interference caused by usin
107. t all Auto increment file name If checked and the captured image is to store in a file the file name will be changed by INFINITY ANALYZE after the current image has been saved The new file name will be used for the next captured image The new file name will CG eport specimen jpg Copyright 2008 Page 137 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 have a integer appended to the original text and this integer will be incremented by one each time the new file name is generated by INFINITY ANALYZE 4 1 5 Light Source The temperature of the light source may be advised to INFINITY ANALYZE to aid color reproduction of the camera The same specimen may appear different under different lighting environment because of the different spectral distribution of the light source The camera may incorporate the factor of light source to produce the right colors comfortable with human perception a Fluorescent O Daylight Incandescent O senon Halogen Other Select to indicate to INFINITY ANALYZE that the light source has a broken spectrum Daylight Select to indicate to INFINITY ANALYZE to add more red Select to indicate to INFINITY ANALYZE to add even more red Select to indicate to INFINITY ANALYZE to add more blue Select to indicate to INFINITY ANALYZE to add more red and blue Select to indicate to INFINITY ANALYZE no color temperature sh
108. te 3 11 5 About Analyze Use the About Analyze command to read copyright notice and version information Page 126 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Other Commands This section details the commands that are not accessible from menu Specifically the Infinity cameras and built in database are most conveniently controlled from Control Panel and Database Window The camera presently connected to INFINITY ANALYZE and the whole image acquisition process can be fully controlled from within the Imaging Control Pane of the Control Panel Image acquisition will be based on the current camera settings and capture options which are configured by the seven sub panes of the Imaging Control Pane described in detail below Camera settings refer to those parameters that are necessary to put the camera into a specific state Capture options refers to what INFINITY ANALYZE will do to the frames grabbed from video stream of the camera including calibration for purpose of measurement and printing In fact system micrometer has to be properly scaled before applying to the newly formed image according to the decimation factor specified in capture options The contents of camera settings capture options and calibration information are listed below Camera Exposure Settings Gain Gamma Saturation fF Hwe S Brightness Contrast fF White Balance Rotation Fp L
109. ted on the bounding rectangle of the annotation object Resizing handles usually reside at the four corners or the center of line segments To detect a resizing handle hover the mouse over the bounding rectangle and wait for the change of cursor If a resizing cursor appears it means the mouse is over a resizing handle Note the bounding rectangle will not appear until the object is selected Each annotation object has a set of properties associated with it The properties may be edited through popup dialogs To change the properties of an annotation object double click that object 3 9 1 Enable Use the Enable command to toggle annotating mode In the annotating mode of INFINITY ANALYZE all mouse actions over the active image except those for annotation are disabled 3 9 2 Line Use the Line command to add line objects Each line object is defined by two endpoints Press left mouse button to specify one of the endpoints Move the mouse to the other endpoint while keeping the left mouse Page 110 S Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual button pressed to draw the line Release the left mouse button to complete the line drawing The above procedure may be repeated to create more lines A line object has two resizing handles located at the two endpoints Properties x Graphics Line Color E Style p Gize 2 ZA _ Round Fen Ea
110. than a few seconds Page 132 d Lu menera Copyright 2008 corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Camera Control The exposure global gain and Gamma of the camera may be set from this sub pane Camera Control Exposure m Exposure ms Use the slider to adjust the exposure time of the camera The 5 exposure is in milliseconds and is adjusted logarithmically IW o o The minimal exposure time allowable for the camera 75 900 Show the current exposure time of the camera A new value may also be entered directly The exposure is in milliseconds The maximal exposure time allowable for the camera G LU ain Use the slider to adjust the global gain of the camera The global gain of a camera is the factor multiplied to the intensity channel of each raw pixel The minimal gain allowable for the camera Show the current gain A new value may also be entered 3 directly 930754 The maximal gain allowable for the camera Use the slider to adjust the Gamma of the camera Minimal Gamma allowable for the camera show the current Gamma of the camera A new value may also be entered directly Page 133 Copyright 2008 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Maximal Gamma allowable for the camera On any INFINITY3 1 camera model this feature option will be available Select the checkbox to enable the cooling function on the
111. the camera manipulation commands also reside here 3 1 1 New for Video Preview Use the New for Video Preview command to create an empty image window to display live images from the camera The caption of the created window is the model or name of the camera presently attached to the computer If no camera is connected or none of the connected cameras is working properly the caption of the created window is Video The camera serial number is appended to the window caption after a dash Copyright 2008 Page 25 Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 3 1 2 Open Use the Open command to load existing image files Multiple images from a single folder can be opened simultaneously whether they are the same or in different image formats Besides the native image format sif numerous commonly used image formats are supported Files of unsupported formats will be filtered out and will not be visible in the dialog box Equivalent Commands e Drag one or more selected files in Windows Explorer and drop them in the main window of INFINITY ANALYZE e Double click a file of native image format sif in Windows Explorer The application recognizes the following image file formats Extension calibration data Group decompressing Format Portable Bitmap Reading both ASCII and binary formats Portable Gray Map Reading both ASCII and binary formats Portable Network PNG Graphics Portable Pixel Map PPM
112. time to obtain the ideal image intensity The preset will be deselected to reflect the change The position of the Exposure slider will also be updated to synchronize with the current setting Use the White Balance command to correct color reproduction aberration of the Camera The color correction is based on the visible portion of the live images For the best results it is advised to perform white balance when imaging a white gray or colorless and bright but not saturated object This command initiates a one push effort to reproduce the right colors It might be necessary to repeat the process until the desirable state is reached Equivalent Commands 1 From Settings File Upon startup INFINITY ANALYZE automatically loads and applies the camera color correction settings used last time These settings and many others are specific to each camera and are stored in a file that is uniquely identifiable by the serial number of the camera When INFINITY ANALYZE exits it automatically updates the settings file 2 From Capture Control Sub Pane Copyright 2008 Page 33 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 mh Eu 400 Fluorescent wi Preset Add White Balance Auto Exposure Amawe J Capture Video On C Video Of 400 Fluorescent White Balance Camera presets include camera When White Balance button is color correction settings Ifa pressed INFIN
113. tion where each input value maps to the identical output value An inverse look up table is shown by a line sloping down from the upper left corner to the bottom right corner resulting in all minimum input values being transitioned to maximum output values and vice versa such that the channel becomes a negative representation of the original enya s WEBI Y The LUT can be modified either graphically or numerically To define the LUT graphically click the mouse on the one end of the line displayed on the histogram There are two control points available to manipulate into a slope that produces the desired result Each of the control points can be manipulated individually by clicking either on the square icons on the line or on the line itself to the outside of the control points Click on the line between the control points and drag the graphic to perform translational adjustments to the line slope The histogram is adjusted to display the LUT adjusted image pixel intensities Enable Disable the LUT on the Live Image Reset the active LUT channel Open the LUT editing dialog Make the Red channel active Make the Green channel active Make the Blue channel active Ea Activate all three channels Copyright 2008 Page 21 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Define Lookup Table Control Pants a eg OK vi fois e Jong Invert Cancel vl 0 433071 pa 0 645663 Al
114. ton changes to the Abort button If it is pressed the computation will be terminated with whatever the intermediate image left as the active image The table in the middle of the dialog box shows the displacements detected from the input images The first column shows the index of the image in Field Group that has just been registered and fused The second column shows the horizontal displacement detected and the third column shows the vertical displacement detected The fourth column shows the time in milliseconds used for the registration and fusion When 0 is specified as the MSD i e when fusing images acquired from a compound microscope no registration is performed and 0 is set as displacement When a non zero MSD is specified i e when fusing images acquired from a stereomicroscope and when the detected displacement is equal to MSD the specified controlling parameter might be too small and the computation should probably be terminated and re started with a greater MSD The progress of the computation is shown at the bottom of the dialog box Copyright 2008 Lumenera corporation Page 68 d Lumenera INFINITY ANALYZE 329 completed 25s to go A new image window will be created to hold the output image The output image has the same metrical and magnification settings as those of the first image in the Field Group The example below illustrates the process The two images on the left are the first and the l
115. tton to ix Ft The Help Desk provides context sensitive information for commands that involve extended mouse actions Database Database D o e E Search in A inclusion 1 Caption Description Inclusion 2006 04 23 16 13 35 John Mon metal inclusion in 2 Tissue 2006 04 22 18 01 25 Jack The haze illustrates spherical aberration It cannot be L Bone 2006 04 22 16 35 42 Jack Radiograph processed as pho lt LI gt The Database panel provides full control over the built in database Some commands may only be available from this auxiliary window For example click a column header to sort the list This command is not accessible from the application menu Measurement Copyright 2008 Lumenera corporation Page 18 S Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual Measurement Parameter Caliper Distance 105 46 Area Perimeter rea 6595 50 Area Perimeter Perimeter 339 68 Area Perimeter Area LU UU Area Perimeter Perimeter 511 42 This auxiliary window is used to hold measurement results A row is also referred to as an entry As illustrated above an entry consists of three cells namely the command that generates the entry the parameter that is being measured and the measurement result A measurement command may generate more than one entry because two types of parameters may be measured with a single command Entries in the Measurement window may be highlighted using the mouse a
116. uble click on the active image being measured 3 8 3 Grid Use the Grid commands to draw a lattice on the active image The Grid gt Line draws a rectangular lattice on the overlay layer of the active image Page 100 d Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual The Grid gt Circle draws co centric circles on the overlay layer of the active image 3 8 4 Grid Options Use the Grid Options command to specify how grids are to be drawn Options for Grid Rectangular wi Spacing A Cancel Cocentnic A adius Ae Radius Increment Ae b Ed The distance in microns Spacing between neighboring parallel lines of the rectangular grid The radius in microns of the innermost circle of the co centric circular grid 29 The difference of radius in Radius Increment microns of neighboring circles of the co centric circular grid Push to close the dialog and e keep the settings Push to close the dialog and discard any change to grid options 3 8 5 Light Density Copyright 2008 Page 101 Lumenera INFINITY ANALYZE Use the Light Density command to profile the intensity distribution of a row of pixels Push down the left button of the mouse to start profiling A horizontal line will be drawn passing through the position of mouse cursor The intensity distribution of those pixels of the active image on this line will be profiled This line also st
117. value may also be entered directly The default value 1 does not modify the saturation of the camera The maximal saturation applicable to the camera The minimal hue applicable to the camera Show the current hue of the camera A value may also be entered directly The maximal hue applicable to the camera Page 76 Copyright 2008 Lumenera corporation Ch Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual w OF Push to dismiss the dialog and commit the changes of settings to the present camera Push to dismiss the dialog and discard any change of settings to the present camera 3 6 4 More Use the More commands to increment by a predefined amount the intensity saturation or hue of the active image or the camera When the active image is an empty image and is being used to display video streams the commands increment the gain saturation and hue settings of the presently connected camera instead of the intensity saturation and hue of a still image The amount of change for each color channel may be specified by the Increments command 3 6 5 Less Use the Less commands to decrement by a predefined amount the intensity saturation or hue of the active image or the camera When the active image is an empty image and is being used to display video streams the commands decrement the gain saturation and hue settings of the presently connected camera instead of the intensity saturation and hue o
118. ve the overlay view currently superimposed on the picture Choose Overlay again to restore This command affects the visibility of the overlay layer only and does not delete the overlay 3 3 6 Overlay Color Use the Overlay Colors command to set a new color for the overlay layer The overlay layer can be shown in different colors after it have been created To select a new color choose Overlay Colors and pick up a desirable one This command is special in that a dialog appears in place of a popup menu item as shown below Copyright 2008 Page 49 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Overlay Color O nima l Automatic Overlay Size EEEREN Se eae eee Corner Soa eee ii ft ft wi Fit to Window PILILILIG More Colors 200m Preview The dialog is initially docked to the menu item Overly Color however if desired it can be made floating by dragging its grip Overlay Color Automatic More Colors The dialog in two different dock states behaves in exactly the same way Click Automatic to select the color white as the overlay color Click any of the color boxes to select that color as the overlay color Click More Colors for more choices or to create a new color Click More Colors will bring up another dialog as shown next Page 50 Copyright 2008 Lumenera INFINITY ANALYZE Colors Standard Custom C
119. window Control Panel The Control Panel provides access to all camera functions and image acquisition commands It also controls image capture options and how acquired images are cached Two panes Imaging Control and Field Group comprise the Control Panel The Imaging Control Panel is further divided into seven sub panes for specific aspects of the image display and capture process Sub panes are collapsible To collapse a sub pane click the arrow in its top right corner To expand a collapsed sub pane click the arrows in the top right corner again The arrows at the top left corner of a sub pane point upward to indicate the expanded state and downward to indicate the collapsed state When INFINITY ANALYZE is launched the displayed panes in the Control Panel are restored to the state they were in when the application was last closed Control Panel y E D fat Imaging Control hi Field Group EI Imaging Control Preset Add Vi rite Balance ZZ Area T l Capture VideoOn 1 Video Off Kee pane Click to go to the next pane Oo to go to Field Group Copyright 2008 Page 17 DXI Lumenera corporation Lumenera INFINITY ANALYZE User s Manual Release 4 6 0 Click to go to a pane directly Imaging Control Field Group E Imaging Control Help Desk Light Density Move curser up or down over the image with lett button down to select the line tor density profiling Release lett bu
120. ystem micrometer Use the Update button to apply a change in camera settings to an existing Preset Remove Most Important Camera Settings Color reproduction and overall brightness dictate image quality The color reproduction of a camera is controlled by gains applied to color channels of the camera It is most convenient to let INFINITY ANALYZE determine the necessary gains from live images a process known as white balance White balance may be based on either the whole live image that is currently being displayed or a small area therein INFINITY ANALYZE uses the term White Balance for the operation based on the whole image and Area Based White Balance Area WB for the operation based on a small area within the live Page 130 d Copyright 2008 Lumenera corporation Lumenera INFINITY ANALYZE Release 4 6 0 User s Manual image Much like white balance an optimal exposure time may also be determined by INFINITY ANALYZE Auto Exposure refers to the operation that INFINITY ANALYZE determines and sets an optimal exposure time based on the visible portion of the live images being displayed white Balance Push to perform white balance based on the visible portion of the live image Push to perform white balance based on a small rectangle within the visible portion of the live image The operation is completed by a click on the live image which specified the center of the rectangle on which the white balance w

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