The Genevac Mega 980 &1200 Max Evaporation Systems User
Contents
1. If you wish to end the work session on the system go to the Select Run screen and press STOP SELECT RUN This prompt will be No Name onluent Vol Holder T Hxt displayed on the SELECT 91 Exanple Et c Hex inl Deep Ue 40 o RUN screen gz Press Y to close the system down or N to select 33 PURIFICATION WATERSACETON I TRILE 10m 18 X 15 another run 94 SOLID PHASE SYNTH TFASDCH eml DEEP WE 95 COMPOUND pF CONFIRM REQUEST 96 OLIGO SYN Shut doun evaporator Are you sure Y N 97 SOLUTION 38 SOLUTION PHASE 2 DCH HETHAHUL iml DEEP WE 99 MAINTENANCE TEST 1 Om MAINTENANCE TEST 2 Om GENEVAC LTD MENU iii EDIT GRAPH CLEAR COPY TO DRAIN DEFROST FLUSH OPTIONS HELP select Run with Tl Menu Item with keys press START to execute starts selected Run using parameters stored e The SHUT DOWN screen will be displayed when you select the Y response e The pump will be purged of solvent vapours for 30 minutes e You can restart the run by pressing START at any point e You can abort Shutdown by pressing START at anytime e Ifyou want to start a different run press STOP select N and press ESC to display the SELECT RUN screen AA QC Jj Antr CY B ssue 2 2 October 200 8 Page 20 of 4 USER MENU e The USER M2. When the run is finished after pressing a key the following screen is displayed RUN 91 Mi Exanple ALARMS Channel 1 Temperature L CI Channel 2 Temperature L C Chamber Tenperature L C Condenser Gas Temp ad I Pressure Cnmbar 11357 Spin Rate Lrpn H Total HH 48 15 End of Run 4H HH AH Channel 2 Pressure ane Rate mbar OO 40 00 45 o0s50 Select Option or press SIUP to skip to select next run Select the DRAIN or DEFROST OPERATIONS option using the numeric keys Press ENTER to display the DRAIN or DEFROST MENU VARIABLES ALARHS e With the DEFROST uiam M MEE CYCLE highlighted press rain Valve anua Flush Valve Not Fitted ENTER to start the defrost Isolation Valve Closed cycle Vent Valve Open Rotor Stopped e his will automatically Chanber Heat Off open the defrost valve Spin Rate rpnl PN nies e Drain the condenser after Chanber Temperature C every run and at the end of Condenser Gas Tenp C the day A defrosted and drained Defrost d Completed when Elapsed Time oe epee Defrost more efficiently Drain Done e Periodically check the level of solvent in the containers attached to the drains Press START to commence DEFROST sequence STOP to cancel Screens similar to this are also displayed for DRAIN and if fitted the FLUSH option
3. DISPLAY Operating the SETTINGS key displays the RUN DATA screen only for the selected run Note that the RUN DATA screen can also be accessed from the menu VIEW EDIT DETAILS option on the SELECT RUN screen when a run is not in progress i m 2 iO o The following notes describe the function of each of each screen and provides an explanation of the prompts displayed 04 4542 Issue 2 2 October 2008 Page 11 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems SELECT RUN No Name 0 l vent 1 Oligosynthesis nmonia 2 Oligo Purification Hater ACH 3 PCR Purification Hater ACN 4 RNA Purification 5 Oligo Production Aqueous Mixture dnl 5H nl T 4H A Hater DCEHEUF LTD MENU START EDIT GRAPH CLEAR COPY TO DRAIN lags HELP Select Run with Tl Menu Item with keys press START to execute Starts selected Run using paraneters stored 04 4542 Issue 2 2 October 2008 FLUSH OPTIONS 5 5 What the screens do There are three main control screens The SELECT RUN screen lists the evaporation profiles you can choose library of This screen has the capacity to store 100 different profiles from which you can select a particular run or set of runs The screen is accessed after switching on the pump and evaporator and by pressing the START key on the keyboard You store and select your runs on these screens Use the UP and DOWN cursor keys to move between profiles Un
4. Lid will not open system powered up 04 4542 Issue 2 2 October 2008 Probable cause Check the security of all the clamps on the system If these are obviously tight and in good condition apply the following procedure Use a process of elimination to isolate the leak you will need a separate vacuum gauge with a resolution of better than 0 5 mbar contact Genevac for details Disconnect the pump from the connecting tube and connect the vacuum gauge transducer directly to the vacuum port Start the pump and note the pressure after three quarters of an hour This should be better than 1mbar If not contact Genevac Service for advice If the pump is satisfactory reconnect the connecting tube from the condenser and disconnect the tube at the condenser end Connect the vacuum transducer directly to the end of the tube and switch the pump on If the pressure is no better than 1 mbar replace the tube If the tube is satisfactory reconnect the tube to the condenser and restart the system If the same conditions prevail contact Genevac Service for assistance Probable cause Contact Genevac Service for assistance Probable cause Check the circuit breaker on the mains supply to the system and reset if necessary Check switches If the fault persists contact Genevac Service for assistance As above As above Contact Genevac Service for assistance Page 40 of 44 Technical data Mechanical data
5. 04 4542 Issue 2 2 October 2008 Page 26 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 04 4542 Issue 2 2 October 2008 Note that the term TxD indicates that the transmitter is not transmitting and will be displayed when the rotor is not rotating The control temperature can be changed during a run by pressing the SETTINGS key to display the Run Data screen Use the cursor keys to select the SampleGuard Control Temperature field and press ENTER Input the new value and press ENTER again The system will prompt you to confirm the change when you leave the Run Data screen Press Y for yes and N for no To verify the calibration of SampleGuard remove the Stuffer Plug shown connect the remote power lead and connect probes to channels 1 and 2 With the lid open immerse both probes in a beaker of water at approximately 40 degrees Centigrade Press the START key and verify the indicated temperatures against a calibrated X digital thermometer The frequency of verification and record retention should meet the requirements of your ISO EN BS 9000 series or NAMAS procedures On removal of the remote power lead always ensure that the Stuffer Plug is replaced SampleGuard will not operate without the Stuffer Plug Page 27 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems AN When flushing the condenser Start venting the chamber prior to carrying out the flush This prevents f
6. 5 2 How to switch on the evaporator Switch the mains switch on The screen will display the Software Control screen Press the START key on the keyboard to access the Select Run screen The Select Run screen will load up at the last run that was used The system will not be available for use until the vacuum pump has reached the correct operating temperature 5 2 How to switch on the evaporator Switch the mains switch on There will be a slight delay until the condenser reaches the correct temperature Page 10 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 5 4 Using the keypad The Series Il keyboard controls and displays have been designed for ease and simplicity in use The keyboard has the most used function keys on the left hand side The right side illuminates when the RUN DATA screen is accessed The START and STOP keys start and stop a START selected run H EB STOP The cursor direction keys move you around the screen in the direction of the arrows The ENTER key is used to open and close a field on the Run Data screen when inputting or amending data ac Note that the START key also acts as an ENTER key when the keyboard is not illuminated Operating the MENU key displays the USER MENU screen only when the rotor is not spinning and the chamber is vented Operating the DISPLAY key displays the RUN LOG screen only when a run is in progress or has finished
7. Please note that it remains the responsibility of the user to consider any solvents being evaporated within the context of the ATEX directive The presence of solvents on the list above indicates only that they will not damage the system If further information is required please contact your Sales Representative or visit 91 Last Used 12 25 03703703 Last Changed 12 21 03703703 Run Data Locked Next Run Number O for nonel Hun Mame sample Holder Type sample Volume ml No 0 Exanp le Deep Well Plate 0 70 Solvent Etc Hex SampleGuard Control Temperature C sampleGuard Control Channel CoolHeat Enable Pressure mbar Chamber Temperature Control C Rotor Speed Heat ff Option Heat ff Threshold End of Hun Option End of Hun Threshold 40 1 200 AUTO Cool to 324 Control at 20 Low Heat Flow Not Applicable Not Applicable Not Applicable Pressure Control Regime Controlled Pressure Controlled Pressure mbar 10 GENEVAC LTD HELP Tl to select ENTER to edit MEMII ESC to exit Select enter name of the solvent s Hun No 91 Last Used 12 25 03 03 03 SOLVENT Hun Data Locked Acetonitrile ACH Next Run Number 0 for none Ammonium Hydroxide MH3DH Hun Name Chloroform CTCE sample Holder Type Di Chloro Ethane DCE sample Volume ml 1 nu TOU PR GRAM I WARK ING Please set control parameters manually Press any key to continue Chamber Temperature
8. Max rotor speed Max Force Drive system Operation imbalance Max load IR lamps number Weight Condenser Condenser temperature Minimum Maximum Vacuum condenser capacity Exhaust condenser capacity Condenser level detector Rapid defrost Condenser chamber Condenser drain valve Vacuum system Pressure display resolution Vacuum control Dri Pure Auto vacuum vent valve Ultimate system vacuum Dimensions Evaporator W x D x H Lid Closed Lid Open Electrical Power supply Environment The following figures apply Operating Ambient Temperature Relative Humidity Altitude otorage Ambient Temperature Relative Humidity Altitude 04 4542 Issue 2 2 October 2008 Genevac Mega 980 amp 1200 Max Evaporating Systems Mega 980 Max 900 Low Speed 1100 High Speed 1100 Dri Pure 300 450G Direct 200g Refer to Page 8 12 1000 kg 45 C 60 C 2 x 8 0 litres 2 5 litres No Yes 316 Stainless steel Stainless steel PTFE 0 1000 mbar 0 1000 mbar Yes Yes 0 4 mbar 1382 x 1084 x 1371 mm 1382 x 1084 x 2313 mm Mega 1200 Max 810 Low Speed 990 High Speed 990 Dri Pure 300 450G Direct 250g Refer to Page 8 24 1300 kg 45 C 60 C 2 x 8 0 litres 2 5 litres No Yes 316 Stainless steel Stainless steel PTFE 0 1000 mbar 0 1000 mbar Yes Yes 0 4 mbar 1600 x 1304 x 1371 mm 1600 x 1304 x 2521 mm 230V 50Hz single phase 25A 5 8kW 208V 60 Hz sing
9. aqueous mixtures at pressures of less than 4 mbar ice can form and extend the evaporation time At a pressure of 6 mbar ice crystals are not formed and the time required for evaporation can be reduced by up to 30 Mixtures of water and methanol in deep well microtitre plates however respond entirely differently as shown by the graphs shown on the next page At full vacuum a reasonable evaporation rate is achieved whilst at 8 mbar the time required for evaporation is increased significantly When evaporating 40 ml fractions in 24 x 150 mm glass tubes or larger volumes in beakers typical evaporation times for water acetonitrile mixtures can be in excess of 8 hours A reduction can be achieved by setting the SampleGuard control temperature to 60 degrees centigrade for a defined period Provided the period was not excessive the sample temperature is likely to remain below 40 C even though the Coolheat lamps remain on as result of evaporative cooling After this period a second or a number of successive runs can be linked at reduced SampleGuard temperatures and different pressures Some experimentation would be necessary to ensure that safe sample temperatures are not exceeded Page 34 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 04 4542 Issue 2 2 October 2008 d zz 7 Care of your system The routine checks and the day to day care points you should be aware of were detailed in secti
10. solvent evaporates the product becomes carbonised by the heat from the lamps and form sites for crack propagation to take place Inspect glasses at regular intervals and clean with a lint free cloth and acetone If the contamination becomes excessive and carbonised as shown contact Genevac Service Genevac manufacture a range of specialised sample holders to protect the Quartz lenses Call Genevac Sales for details Bumping and spitting is unpredictable and may occur with any solvent or mixture 04 4542 Issue 2 2 October 2008 Page 33 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems Limiting the chamber pressure Bumping must be avoided as it is a potential source of cross contamination between samples Bumping can also cause product to be deposited on the glass lenses as previously described Linking Runs 04 4542 Issue 2 2 October 2008 Use the Dri Pure option on the drop down menu under Pressure Control Regime on the Run Edit screen This option incrementally reduces the pressure and automatically sets the rotor speed to high 6 3 Optimising a run This section will describe some of the general principles applicable to optimising evaporation rates by exploiting the flexibility of the Series Il control software Pressure control can be used to significantly increase the evaporation rate of mixtures of greater than 30 water when contained in glass tubes and beakers When evaporating
11. stp Pike 28 5 9 mie TO SUL Ch PNM 29 6 Getting the best from your SYStOM cccccsssceccseseeeceeseeecseuseeecaceeseaeeecseuseeessaseeessageeessegseesssuseeessageees 32 6 1 FROUUING CIC ia ai 32 6 2 FONG ON CVG IO ase sents shee nt grates 33 CUTS IA TO serio cas eps E EE 34 T Ane On e DINI E LT Lm 35 7 1 HOW TO Cican UNG Ci NO OM se m T M M 35 7 2 Maintenance and S CIVICS useiuidesqetoniosunPiaustmepdic ciim bsp osi mass a a 36 c MEE Additonal 16 0 8 812 0 M ete ee eee eee eee a epee ee ne ene eee eee erence eee 37 7 4 MOVING YOUN SY SUNN RN CETT DO 38 8 miU ipn 39 9 Technical dala TE xu E DT 41 Mechanical data m RHHT 41 ie to 0 T aen E E E E E E E EE EE EE R 41 Vacuum Systemie anena E EE N aE E A EAEE TATAA ana aa aa 41 DIMENSIONS m ae a EE EEE ET E E Ea a E E E aE a 41 Geri A POE A VEE A E N A E E E E E E 41 OO ra E E E EE E EE eee eer Ter 41 04 4542 Issue 2 2 October 2008 Page 2 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems Contents Continued 10 ze pIeririejeg amp egeimve m H 42 11 rj 42 12 Warranty Statement serse
12. Applicable Pressure Control Regime Controlled Pressure Controlled Pressure mbar 10 GENEVAC LTD HELP TL to select ENTER to edit MENU ESC to exit Temperature to maintain using SampleGuard Hun Ho 91 Last Used 12 25 03 03 03 Last Changed 12 31 03703703 Hun Data Locked Ho Next Run Number 0 for none 0 Hun Mame Example sample Holder Type Deep Well Flate sample Volume ml 0 70 Solvent EtAc Hex SampleGuard Control Temperature I CHAMBER TEMPERATURE SanpleGuard Control Channel CoolHeat Enable Pressure mbar select Chamber Temp control regime Hotor Speed Wait for Chamber to Heat Wait for Chamber to Cool Heat Off Option Heat Off Threshold End nf Hun Option End nf Hun Threshold Not Applicable Controlled Pressure Pressure Control Regime Controlled Pressure mbar 10 GENEVAC LTD HELP TL to select ENTER to accept ESC to cancel Chamber temperature controlled to suit selected solvent or mixture fy la Coria CYrtebesaer nno ssue 2 2 October 2008 SampleGuard Control Temperature oet within the range of 20 to 70 C SampleGuard Control Channel Channel 1 in Swing or Sample Holder Channel 2 in Sample Coolheat Enable Pressure oet within the range of 5 400 mbar Chamber Temperature Control There are now 3 options available Wait for Chamber to Heat Range from 0 C to 45 C Wait for Chamber to Cool Range from 0 C to 45 C Automatic Control Temperatu
13. Cont Rotor Speed NMP Heat Off Option Tri Ethyl Amine TEA Heat ff Threshold TFA End of Hun Option IHF End of Hun Threshold Toluene PhMe Water HZ0 Pressure Control Regime BP 50 Controlled Pressure mbar BP gt 1860 Other Clear Done GENEVAC LTD HELP TIYENTER to select deselect select Done when finished ESC to cancel EtAc Hex With Auto Program Y es selected The screen will now display the Auto Programmed data in Light Blue Save Run and continue If you did not select Auto Program or were not given the option due to inputting an unrecognised _ solvent then you will be presented with X this screen If editing an existing run parameters will remain as previous settings If creating a new run parameters will be set to safe default settings Page 15 of 4 Hun Ho 91 Last Used 14 40 03 03 03 Last Changed 14 46 03703703 Hun Data Locked Ho Next Hun Number O for nonel Hun Name Example sample Holder Type Deep Well Flate sample Volume ml 0 70 50 l vent Et c Hex SampleGuard Control Temperature C 40 SanpleGuard Control Channel 1 CoolHeat Enable Pressure mbar 200 Chamber Temperature Control C AUTO Cool to 32 Control at 20 Kotor Speed Low Heat Off Option Heat Flow Heat Off Threshold Not Applicable End nf Run Option Not Applicable End of Run Threshold Not
14. ENU is displayed by pressing the MENU key only when the chamber is vented and the OPTIONS rotor stationary OPERATOR CONTROLS HA INTENANCE e Lamp Layer Selection is not an option for the HT 4 or Mega e In Operator Controls you can adjust the screen brightness to suit your viewing angle e You can turn the audible warning off or on GENEVAC LTD e Press ESC to leave this HELP Tl to nove to required option ENTER to select ESC to leave this menu Option not available in this node or configuration menu CONTROLS MENU Ad just Screen Brightness Reupress Beep nct nes Use the cursor keys to select User Intervention Beeps On Off Off the OPERATOR CONTROLS and press ENTER End of Run Beeps On Off Off e Use the UP and DOWN cursor keys to adjust the screen brightness to suit your viewing angle e Toggle the beep ON or OFF e Press ESC to exit this screen GEHEURC LID HELP Tl to move to required option ENTER to select ESC to leave this menu Adjusts brightness and optinum screen viewing angle 5 6 How to enter control data All control data is entered from the RUN DATA screen From start up of the system and the software control screen press START to display the SELECT RUN screen Select the VIEW EDIT DETAILS option and press START to display the RUN DATA screen Use the UP and DOWN cursor keys to move between fields Unless otherwise stated press ENTER to open a field input y
15. OT BE OPERATED BY PERSONNEL WHO LACK THE TRAINING OR PROFESSIONAL EXPERIENCE TO COMPREHEND THE HAZARDS THAT CAN ARISE WHEN USING THE SYSTEM Personnel without such training require thorough instruction These operating instructions should form the basis of this instruction 3 4 Positioning the evaporator POSITION THE EVAPORATOR AT LEAST 300 MM AWAY FROM THE EDGE OF A BENCH AND THE SAME DISTANCE CLEAR OF BREAKABLE OBJECTS OR AREAS WHERE ENTRAPMENT COULD OCCUR Page 7 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems There are important safety and operational considerations to be made when positioning the system Refer to Section 9 Technical Data for recommended clearances MEGA 980 Max 9 6kg MAX including adaptor plate tubes solvent sample sample holder BUT excluding the swing MEGA 1200 Max 17 8kg MAX including adaptor plate tubes solvent sample sample holder BUT excluding the swing Always evenly distribute the tubes in the sample holders Genevac Ltd will accept no responsibility for any loss or damage incurred by improperly or excessively loaded rotors 04 4542 Issue 2 2 October 2008 4 Safety BEFORE OPERATING THE SYSTEM IT IS IMPORTANT THAT THE FOLLOWING NOTES ARE READ TO ENSURE THAT THE IMPLICATIONS TO THE SAFETY OF PERSONNEL OPERATING THE SYSTEM AND FOR THE PROTECTION OF SAMPLE INTEGRITY ARE UNDERSTOOD Samples in the chamber are subjected to accelerations of up to 450G w
16. TO IEC CLASSIFICATION IT MUST NEVER BE USED WITH ANY INTERRUPTION TO THE SAFETY EARTH CONDUCTOR IT IS AN INSTALLATION CATEGORY II PRODUCT AND IS INTENDED TO OPERATE FROM A NORMAL SINGLE PHASE SUPPLY THIS EVAPORATOR HAS BEEN DESIGNED TO BE USED IN A POLLUTION DEGREE 1 ENVIRONMENT NO POLLUTION OR ONLY DRY NON CONDUCTIVE POLLUTION ANY MAINTENANCE OR REPAIR OF THIS PRODUCT SHALL BE CARRIED OUT BY GENEVAC _ PERSONNEL OR _ _ APPROVED REPRESENTATIVES OF GENEVAC USING ONLY APPROVED SPARE PARTS Page 42 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 12 Warranty Statement This product is guaranteed for period of 12 months from the date of delivery to site In the unlikely event of any defect arising due to faulty materials or construction resulting in system failure the unit will be repaired free of charge This to include all labour and component costs incurred This warranty is subject to the following provisions 1 2 5 System must be sited installed and operated in accordance with operator instruction manual Unit only used for purpose it was sold and in accordance with Genevac published compatible solvent list Regular cleaning and preventative maintenance schedule to be adhered to as detailed in operator s manual Warranty does not cover accidental damage misuse modifications or inappropriate repair by untrained personnel Warranty does not cover consumable items Failure to adhere to
17. User Manual Issue 2 2 October 2008 Part Number 04 4542 Genevac Mega 980 amp 1200 Max Evaporating Systems Contents 1 Jrugejs eis c 4 Amendment Sro igo Maro ep ED OE SERRE 5 1 1 A y DOS ere E E E AEE 5 Genevac Evaporators and Combustible Solvents ccccccccccsecceceeeeeseeeeeeeceeaeecesaeeeeseeeesseecesseeeesseeeessnseeseeeenas 5 2 System description and OD NOMS cci ox o rbetecle trc InE EE au dee e EMPcbee Ei xau ede UN Eod eH o Genes uavn lt elacdoueaceda edeceunetecestecoans 6 3 Scope of delivery and installation seeelssssssssssssssseeeeen nnnm nennen nennen nennen nnn 7 3 1 Checking the CSUN m 7 3 2 Arranging COMMISSIONING sssssssssssseeseeeeeneeen nennen nennen e nnne earns earn n se sns es a nsn nnn ar nnne nnne T MUNN e E E E E nate T 3 4 F osimonimng ME CYApPOTI TON seisis ennn Sea E EN R EEE EEEE TENE T 4 E E E h TEA 8 4 1 SOAM TOO DNI 8 S MEE e 9 8 aO eaen E E E E EE E EE 9 5 SS TIT SE MUU E E AT A AT T E E EA E E EE A E N E E 10 5 1 How to switch on the pump sssessssssssesseeennnnennnen nennen nnnm nmn nnn nnnnn nnn nn nna nnne nnne sre sr nna rn sna nns 10 5 2 How to switch on the evaporator sssssssssssssssseseeee nennen nnn nnn
18. c Control Temperature is automatically set to an optimised figure for the recognised solvent mixture selected Input the minimum temperature you want the chamber to reach before spin up will commence 04 4542 Issue 2 2 October 2008 Page 23 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems Rotor Speed Press ENTER to display the Select Rotor Speed drop down menu Use the UP and DOWN cursor keys to select Low or Lyophilise is not available on a Mega High or Lyophilise Not available on a Mega Low speed applies nominally 300 G and high speed nominally 500 G Selecting the Dri Pure option described later will over ride this option and sets High If nothing is selected the setting will default to LOW speed Select either Elapsed Time Heat Flow or Sample Heat Off Option Detection For Elapsed Time enter value in hours and or minutes Heat Flow is not currently available on a Mega Only requires 1 SampleGuard probe This MUST be used to control the sample holder or swing temperature Not currently available on a Mega Either channel 1 or channel 2 may be used ensure that the channel chosen is selected as the control NOTE channel in the run options Control Channel Probe MUST be placed in the swing OR SAMPLE HOLDER Automatically detects dryness by monitoring the rate at which the solvent is evaporating Low solvent loading or if the lamps are turned off may cause Heat Flow to fail If failur
19. ch full vacuum and the full vacuum achieved This data will give an indication of the performance of your system 04 4542 Issue 2 2 October 2008 7 2 Maintenance and Service Every effort has been made during the design and manufacture of your system to assure the build quality and reliability of your system Over a period of time however there will be some degree of wear and ageing of the seals and bearings in the chamber condenser and pump The extent of wear and ageing will depend on the utilisation of the system the severity of temperature cycling and the nature of the solvents being used With some solvents pin hole corrosion may occur in the connecting tubes which will result in a decline in performance Drops in performance are manifested in the time taken to reach full vacuum the level of full vacuum and a general decline in evaporating efficiency In order to keep your system at peak performance and to avoid costly and unscheduled down time we strongly recommend some form of planned maintenance Because of the complexity of changing parts in the field a high level of skill is required to maintain a complete system To this end Genevac can offer a range of preventative maintenance service and breakdown contracts to cater for your particular needs Page 36 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems T 3 Additional equipment There may be future applications for which your curr
20. d of Run Elapsed Time HH MM 00 00 e Select your sample holder type or if not Pressure Control Regime Dri Pure defined select Final Controlled Pressure mbar 0 Other and enter the details GENEVAC LTD Sample Volume HELP fi to select ENTER to accept ESC to cancel e Select and enter Deep Well Plate value s Coin CYrtebesaer QNNQ Dann 19 LA ssue 2 2 October 2008 Page 13 of Run No 91 Last Used 12 25 03703703 SOLVENT Solvent Run Data Locked Acetonitrile ACH Next Hun Number O for none Anmonium Hydroxide NH30H Run Name Chloroform CTCE When you select this Sample Holder Type Di Chloro Ethane DCE field you are given a list Sample Volume m1 DCM of solvent types that Di Methyl fcetamide DMA DMF have already been SampleGuard Control Temperature C DMSO programmed into the samnpleGuard Control Channel Ethanol EtOH evaporator CoolHeat Enable Pressure mbar Ethyl Acetate EtAc Chamber Temperature Control C Rotor Speed Methanol MeOH Use cursor keys to NMP move up and down Heat Off Option Tri Ethyl Amine TEA Heat Off Elapsed Time CHH MM TFA End of Run Option THF Press ENTER key to End nf Run Elapsed Time HH MM Toluene PhMe select Highlighted in Mater HZ0 blue Pressure Control Regime BP 50 Final Controlled Pressure mbar BP 180 Other Please note that the he solven
21. ding evaporation of such solvents particularly with respect to the European ATEX directive is available on our website or from your local sales representative IMPORTANT THIS SYSTEM MUST BE EARTHED SEE PAGE 42 04 4542 Issue 2 2 October 2008 Page 5 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 2 System description and options Your Mega Evaporation System is comprised of an evaporation chamber and rotor combined with a cryopump condenser unit Vacuum is provided by the Scroll Pump although other types of vacuum pumps can be used Solid state case heaters and Coolheat radiant lamps heat the chamber and samples The control of chamber bucket and sample temperature vacuum ramping rate chamber pressure rotor speed and run time are all handled by an embedded PC For the first time the end user has the facility to auto program run parameters for any recognised solvent mixture entered These optimised run parameters are highlighted in Sf on the display screen when this Auto Program facility has been selected Recognised Solvent s available from drop down menu Sample Holder type available from drop down menu The Run Progress screen now has a fully featured graphical display providing annotated plots for temperature and pressure now a log scale The control software enables the user to specify and store a library of up to 100 different evaporation profiles in an uncomplicated manne
22. e occurs select one of the other two options For very low solvent loading requiring very little heat then the following would be used Sample Detection Requires both SampleGuard probes Channel 1 to monitor the swing holder temperature and Channel 2 in the centre well of the sample holder to monitor sample temperature Monitors the temperature difference between the 2 channels At the start they are the same but as the samples dry the temperature difference gets smaller When the temperature difference reaches the preset figure Not user configured the run is terminated 04 4542 Issue 2 2 October 2008 Page 24 of 44 Q D D lt t O D e t9 c CO e Qo N e e Q X m t oO t e o lt o e D 3 o This can be in a range of 0 lamps off for the entire Meat OIT Elapeed time run to 99 hours 59 minutes End of Run Option This option is the elapsed time after which the run will be stopped End of Run Elapsed Time Press ENTER and input the time after which you want the run to stop This can be in the range of 1 minute to 99 hours 59 minutes Pressure Control Regime Press ENTER to display the Select Pressure Control Option drop down menu Use the Up Down cursor keys to select the pressure option required Full Vacuum Full Vacuum will take the chamber pressure down to the vacuum capability of the pump Controlled Pressure When the Controlled Pressure opt
23. e wrong channel refer to section 5 7 How to use SampleGuard e The Coolheat enable pressure has been set incorrectly refer to section 5 6 Coolheat Enable Pressure e The lamps have not been enabled refer to section 5 5 Lamp Layer Menu e The heat off elapsed time has not been specified or specified incorrectly refer to section 5 6 Heat Off Elapsed Time Probable cause e The chamber pressure may be controlled refer to section 5 6 Controlled Pressure e Dri Pure may have been selected refer to section 5 6 Dri Pure e here may be a leak in the system refer to Leaks below Probable cause e You may have inadvertently linked a run refer to section 5 6 Next Run Number e You may have inadvertently looped a run refer to section 5 6 Next Run Number e Check that the condenser pot in use has been drained refer to section 5 8 How to use the condenser e Check the sample holders and contents for weight differences of greater than 50g refer to section 4 1 Safe loading Page 39 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems Specific Suspected leak pressure of no better than 5 mbar Be sure to check that you have NOT limited the pressure setting Check the pressure setting regime before proceeding any further Condenser Condenser not getting to temperature 40 C Electrical No power to any part of the system No power to the pump and condenser No power to the chamber
24. emperature reaches the control temperature stop the system and inspect the samples Other evaporation run profiles are given in section 6 3 Optimising a run Page 31 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 04 4542 Issue 2 2 October 2008 6 Getting the best from your system 6 4 Routine Checks For high boiling point solvents such as DMSO NMP DMF and DMI the best evaporation rates will only be achieved at pressures better than 0 5 mbar Check all clamped joints on a regular basis to ensure that they are secure Always ensure that the condenser pots are drained before every run Flush the condenser pots with a suitable solvent at regular intervals Check the catch pot connected to the pump exhaust on a regular basis and drain as necessary Never pump a heated condenser containing traces of liquid DMF DMSO or NMP as this will contaminate the pump oil Check the pump oil level initially on a daily basis until a usage pattern has been established and then once weekly CVP Only Ensure that the oil level is within the notch on the dipstick and no lower than the middle of the notch CVP Only Keep a weekly log of the time taken for your system to reach full vacuum as this data will give you an indication of seal ageing and wear With high boiling point solvents it is advantageous to pre heat aluminium sample holders before commencing a run to increase evaporation speed Use the li
25. ent range of sample holders are not adequate The following accessories are just an example of the many ways in which Genevac can assist you in developing your system for the future For further information or to discuss any ideas you may have please call Genevac Sales All sample holders are of a solid aluminium construction and black anodised to improve heat absorption Maximum contact area provide good physical support optimum heat transfer and even heat distribution Solid aluminium tube holders provide even heat distribution for uneven drying loads FastStack for shallow well plates Side Bridge Medium length tube holder Short tube holder FastStack for deep well plates Flask holder 04 4542 Issue 2 2 October 2008 Page 37 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 7 4 Moving your system These notes are included to assist you in moving your system to another area Before moving your system the following key points should be addressed e Ensure that there is sufficient floor space to position your system e Ensure that there is an adequate power supply e Ensure that there is adequate ventilation e Ensure that there is sufficient clearance for adequate ventilation of the pump and condenser e Ensure that provision has been made for the drainage of condenser solvents and the pump exhaust e Ensure that the condenser pots are drained and flushed e Ensure that the sample swings are
26. essure Controlled Pressure mbar 10 GEHEURnC LTD HELP TL to select ENTER to accept ESC to cancel switch CoolHeat off when Heat Flow drops below predetermined level fy la Coria CYrtebesaer nno ssue 2 2 October 2008 Rotor Speed Low Rotor speed applies nominally 300 G High Rotor speed applies nominally 500 G Lyophilise Not currently available on a Mega Heat Off Option Elapsed Time Press ENTER and input the time after which you want the lamps turned off This can be in a range of O lamps off for the entire run to 99 hours 59 minutes Heat Flow Not currently available on a Mega Sample Detection Monitors the temperature difference between the 2 SampleGuard channels U Q M O A RUH 981 Example Channel 1 Temperature C Channel 2 Temperature C Chamber Temperature C Condenser Gas Temp c Pressure mbar Spin Rate rpn Total 46 63 64 This Stage 46 43 64 ea ee aplinnlimng Fs T LOOLHeATt Evaporat ing RUN 91 Channel 1 Temperature C Channel 2 Temperature C Chanber Temperature L C Condenser Gas Temp a IE Pressure nbhbar l Spin Rate rpn Total 66 45 43 End of Run 68 82 32 Post Run Final Drying Period Channel 2 Chanel 2 Pressure nus 40 Pressure During a run The main section of the RUN LOG screen gives a graphical display of the real time chan
27. ges in chamber pressure spin rate sample and sample holder temperatures The top left hand section displays the current temperatures of channels 1 and 2 the chamber temperature the condenser temperature the chamber pressure the total time elapsed and the stage time elapsed if linked runs are being undertaken NOTE THE NEW GRAPHICAL ALARMS DISPLAY e The RUN LOG screen displays the real time events e The top left block gives current values Spin Rate nbar e The top right block displays any alarms e The block at the foot of the screen displays the current status of the system and Instructions e Pressure is plotted on log scale e Press STOP to terminate a run at any time When run enters final drying period the display changes to indicate the time left to End of Run or if linked runs End of Stage Press the DISPLAY key during a run to return to the RUN LOG screen Spin Rate mbar Operating the STOP key at any time during a run will terminate the run The chamber will vent and the rotor will start to spin down You will not be able to open the chamber until the chamber has reached atmospheric pressure and the rotor has stopped 00 45 spinning When the chamber has vented and the rotor stopped you will be given this STATUS prompt Run finished press lt gt to view data any other key to end 2
28. housing is stamped next to the connectors to identify channels 1 and 2 The third connection is for a remote power supply to power SampleGuard to verify temperature calibration refer to later notes Always ensure that channel 1 is used to monitor and control the sample holder temperature and channel 2 to control the sample temperature Always use channel 1 as the control channel as using channel 2 under these circumstances could result in damage to thermo labile samples Depending on the solvents being evaporated and the conditions significant cooling and or freezing will normally occur Under these conditions using microtitre plates it is advisable to position the sample probe in a sample well near the centre of the plate Since the central wells are surrounded by other frozen or cold wells there will be a significant thermal drain Under these conditions dryness may well occur in the outer wells whist the central samples remain in solution or frozen When positioning the sample probe always ensure that the probe tip is located firmly at the bottom of the well vial or tube i The use of Genevac Heat Transfer Plates would prove to be beneficial in preventing this condition arising by providing an even heat transfer to your samples Please refer to our Options amp Accessories Brochure to get the best solution for needs Available in pdf format as a download from our web site http www genevac co uk
29. ion is selected the Controlled Pressure field will be displayed Move down to this field using the DOWN cursor key and press ENTER Input the pressure you want the chamber to be limited to in the range of O to 500 mbar DRI PURE reduces the vacuum over a predetermined period to 50 mbar then goes to the control pressure as set by the user This feature is particularly useful in preventing bumping the violent boiling of solvents resulting in solvents being expelled which is a source of cross contamination of samples Bumping can also cause products to be deposited on the glass lenses of the Coolheat lamps which eventually results in breakages Note that during the DRI PURE cycle which is approximately 40 minutes the Coolheat function will be disabled Variable Dri Pure Variable Dri Pure allows the user the option to set 1 The pressure at which the vacuum ramp will start 2 The pressure at which the vacuum ramp will end 3 The total duration of the vacuum ramp 4 Final Controlled Pressure 04 4542 Issue 2 2 October 2008 Page 25 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 5 7 How to use SampleGuard SampleGuard is a dual channel temperature controller that operates when the rotor is spinning Thermocouple type probes are connected to the SampleGuard housing as shown One probe is used to control the sample holder temperature and the other the sample temperature The SampleGuard
30. ith a maximum load capacity of 9 6 kg per swing for a Mega 980 Max and 17 8kg for a Mega 1200 Max The following precautions should therefore always be observed 4 1 Safe loading of rotor For a Mega 980 Max never exceed the maximum load capacity of 9 6kg per swing For a Mega 1200 Max never exceed the maximum load capacity of 17 8kg per swing Balance pairs of sample holders that are loaded opposite each other to within 10g 5g Note that over a maximum imbalance as specified in the Technical Data the evaporator will cut out Locate tubes correctly in tube holders Locate sample blocks correctly in sample swings Load two or four tube holders in opposite and balanced configurations Distribute tubes in sample holders symmetrically Rotate the rotor by hand after loading and check that all tube holders and plates are correctly located before starting a run and before re starting an interrupted run Do not load tubes or vials into sample holders other than those types that have been approved by Genevac Ltd Do not use sample holders that have not been supplied with system without consulting Genevac Service Page 8 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 04 4542 Issue 2 2 October 2008 4 2 Safe Lid operation The lid is opened and closed automatically by an electric actuator To open the lid select OPEN on the LID DIRECTION switch and press both OPERATE switches simultaneou
31. l Holder T ml DEEP UE 35 ml DEEP WE 3H iml DEEP UE 38 By moving across the horizontal menu bar 10ml Z4 X 15 4 You can START a run You can VIEW and EDIT a profile You can CLEAR a profile You can COPY a profile Start to DRAIN Start to DEFROST Start to FLUSH If Option Fitted Adjust your OPTIONS The 100 profiles are shown on this and nine other Screens The other screens can be accessed quickly by moving the cursor down to the last entry on the page and pressing the down cursor key This will take you to the last entry on the next page Alternatively use the up cursor key Page 12 of 44 E NH EE HN EN a E ENS Each line on the SELECT RUN screen is generated from the RUN DATA screen When the chamber is vented and the rotor stationary this screen is accessed by selecting VIEW EDIT at the foot of the SELECT RUN screen and pressing START When the system is running the RUN DATA screen i E can be accessed directly by pressing the SETTINGS key SETTINGS Hun Ho 91 Last Used Not yet used Last Changed New entry Next Run Number 0 for none i Use the UP and Run Name DOWN cursor keys Sample Holder Type to move between Sample Volume ml 0 00 fields 0 lvent SampleGuard Control Temperature C zu d Press ENTER to SampleGuard Contr
32. lder Type Sample Volume Solvent SampleGuard Control Temperature SampleGuard Control Channel Coolheat Enable Pressure Chamber Temperature Control Rotor Speed Heat Off Elapsed Time End of Run Elapsed Time Controlled Pressure 04 4542 Issue 2 2 October 2008 5 9 How to start a run This section will describe a typical evaporation run of DMSO In this example two sets of eight 28 x 60 mm scintillation vials each containing 5 ml of DMSO were held in solid aluminium sample holders SampleGuard control temperature was set at 40 degrees centigrade and the pressure to full vacuum To prevent condensation the chamber preheated to 40 degrees centigrade Was Switch on the pump evaporator and condenser Display the Run Data screen and input the following data Page 29 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 04 4542 Issue 2 2 October 2008 Dispense the DMSO into the vials and load the sample holders into the sample swings Always use the upper level when loading partial rotor loads Always ensure that the vials sample holders and sample swings are correctly and securely located Position the SampleGuard probe in its location hole in the sample holder and connect to channel 1 on the rotor Position the sample SampleGuard probe in a central vial and connect it to channel 2 on the rotor Always ensure that the tip of the sample probe is located at the bottom of the vial owitch
33. le phase 25A 5 8kW 15 C to 30 C 10 60 Sea Level to 1 600m 10 C to 60 C 10 80 Sea Level to 12 000m Page 41 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 10 EC Declaration of Conformity We Genevac Limited Declare that this product Mega Series Il Evaporating System Complies with the relevant Essential Health and oafety Requirements of the European Machinery Directive 89 392 EEC as amended by 91 368 EEC and 93 44 EEC The EMC Directive 89 336 EEC and the Low voltage Directive 73 23 EEC Conformity is demonstrated by compliance with the following Harmonised European Standards EN 60204 1 1998 Safety of machinery Electrical equipment of machines Pt 1 General Requirements EN 294 1992 Safety of machinery Safety distances to prevent danger zones being reached by upper limbs EN 1088 1996 Safety of machinery Interlocking devices associated with guards Principles of design and selection BS EN ISO 12100 pts 1 amp 2 2003 Safety of Machinery Basic concepts general principles for design BS EN 50082 1 1998 Electromagnetic compatibility Generic immunity standard BS EN 61010 2 020 1995 Safety requirements for electrical equipment for measurement control and laboratory use Particular requirements for laboratory centrifuges 04 4542 Issue 2 2 October 2008 11 Safety WARNING THIS SYSTEM MUST BE EARTHED THIS EVAPORATOR IS A SAFETY CLASS 1 PRODUCT ACCORDING
34. lly inspect the probe ends and connections for damage 04 4542 Issue 2 2 October 2008 A brief discussion is given here to give the user a better understanding of the processes involved When the run is started the pressure will drop steadily as air is evacuated from the chamber and condenser With the Coolheat lamps operating from 100 mbar and because the chamber has been pre heated to 40 C the sample holders soon reach the pre set temperature The maintenance of the 40 C ceiling clearly shows the benefit of having and using SampleGuard As solvent starts to evaporate the pressure drop will stop or reduce in rate The temperature of the sample drops due to evaporative cooling Provided there is sufficient vacuum the drop in sample temperature will take place even though the Coolheat lamps are on This will substantially reduce the evaporation rate and the pressure and vapour flow will drop until a steady state is reached There is then normally a long period of constant pressure and vapour flow whilst the bulk of the solvent is evaporated As the solvent approaches dryness the evaporation rate often reduces causing the vapour pressure to drop This is particularly noticeable with oily samples whose vapour pressure is very low At this point the sample temperature will start to rise and reach the SampleGuard control temperature in a reasonably short time A few minutes after the sample t
35. lushing the whole chamber which is undesirable 04 4542 Issue 2 2 October 2008 5 8 How to use the condenser ENSURE THAT A SUITABLE RECEPTACLE IS CONNECTED TO THE DRAIN PRIOR TO SWITCH ON AS DRAINING OCCURS AUTOMATICALLY The automatic condenser VC8000TA is designed to ensure maintenance free and optimised condensing and that a fresh cryopump is always available The condenser starts in automatic mode when powered up switched ON This is indicated by the AUTOMATIC LED being illuminated When initially powered up CONDENSER 1 starts its chilling cycle indicated by a rapid drop in temperature on the display The cryopump pots automatically switchover during the run The temperature indicators and HOLDING switch lamps reflect this Provision has been made to manually over ride the automatic process After switchover the operator is able to hold the condenser on the current pot This could be used to prevent switchover if the end of the run is imminent When the HOLDING button is pressed the AUTOMATIC LED will extinguish the HOLDING LED and the AUTOMATIC switch will illuminate To re enter automatic mode press the AUTOMATIC button the AUTOMATIC button and the HOLDING LED will extinguish and the AUTOMATIC LED will illuminate At which point the drain and switchover cycle takes place Page 28 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems Run Data Locked Next Run Number Run Name Sample Ho
36. lvent Drop down menu Use the UP and DOWN cursor keys to select the option s and press ENTER Once your selection has been made select Done and press Enter you are now given a new option to Auto Program the remaining run parameters If OTHER is selected the name should be no more than 31 characters long press ENTER to return to Solvent drop down menu 04 4542 Issue 2 2 October 2008 Page 22 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems Select Done and press ENTER SampleGuard Control Temperature Press ENTER and input the temperature you wish to limit the sample to during evaporation This can be in the range of 1 to 70 C Note that if not specified the default setting is 30 C Press ENTER and input the channel on which you Sample Guard Control Channel want to control temperature Channel 1 will normally be assigned to the probe in the sample holder and channel 2 to the probe in the sample Note that when powered down this setting defaults to channel 1 as the control channel Press ENTER and input the pressure below which Coolheat Enable Pressure you want the lamps to be turned on This can be in the range of 5 400 mbar If nothing is selected the setting will default to 400 mbar Press ENTER and Select the method from the 3 Chamber Temperature Control options displayed Wait for Chamber to Heat Range from 0 C to 45 C Wait for Chamber to Cool Range from 0 C to 45 C Automati
37. n time rotor and chamber temperatures to be set in an instant and single push buttons set the other functions This manual will guide you through the start up requirements set up needs and operation of the system to facilitate the most efficient procedure to protect your product s integrity and to ensure optimum performance at all times Page 4 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems Amendment Control Form Issue Reason for Change Date Issued 1 Change to EU Declaration of Conformity 21 September 2004 Update U S address 24 October 2008 1 1 Safety symbols The following safety symbols are used throughout this manual The definitions and scope of each symbol is as described below WARNING THIS SYMBOL INDICATES HAZARDS THAT CAN LEAD TO SERIOUS MATERIAL DAMAGE OR POTENTIAL SERIOUS INJURY Caution This symbol provides information about hazards that can be harmful to your health or lead to material damage q Note i This symbol provides information about technical requirements which if not followed can lead to malfunctions inefficiency and reduced productivity Genevac Evaporators and Combustible Solvents This symbol indicates that there may be a risk to sample integrity Please note it remains the responsibility of the user to consider safety when evaporating any combustible solvents and ensure the system is placed in a well ventilated environment Genevac s position regar
38. nk run facility when evaporating solvent mixtures with large differences in boiling point refer to 6 3 Optimising a run Page 32 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 6 2 Problem prevention Condensation of the evaporating solvent will occur when the solvent vapour temperature is above that of the chamber walls This is most likely to occur with commonly used solvents such as NMP DMI DMSO and possibly DMF To prevent this it will be necessary to pre heat the chamber To do this remove the sample holders and set the Minimum Chamber Temperature field to 40 degrees on the Run Data screen and start the run The chamber will take approximately 20 minutes to reach this temperature Note that the Coolheat lamps will not operate during this pre heating cycle If condensation occurs unexpectedly immediately switch off the lamps by entering O into the Heat of Elapsed Time field on the Run Data screen Pre heating the chamber is not recommended when evaporating volatile solvents such as IFA acetonitrile or methanol TFA Creep TFA exhibits the property of creeping which is the movement of the TFA in liquid phase up the inside of the tubes vials or microtitre plates Problems can arise if solvent containing product is deposited in this way on the top face of plates During rotation of the rotor solvent and product are thrown onto the Quartz glass windows in front of the lamps Whilst the
39. nnn sn nsns n nnne rre a nns 10 5 2 How to switch on the evaporator sssssssssssssssseseeeennnn nennen nnne nnn nnn nnn nnne nnn ne rsen nns 10 5 4 Using Me Keypad MET aea ea Ace aay ch Ee a e a one aea Te EENE EE 11 55 WV FAT TNS screens CO era deicincceccsnss vende lenman iiaa ins aa aa aa Dens a e aaa ENET meee 12 Same TIONS le EVDE aea a E O enc asatasssas 13 rz Igel sm OUI RENTE E E E 13 Sl M RTT TT 14 SampleGuard Control Temperature ccccccccsesccceeseeceneeeceececceeeeseecesseeeeseueeeseaeeeseueessageeseeessaeeeseaaees 16 Sample Guard Control CHANING MNNMTET m 16 Coolheat Enable Pressure cccccscccccsscecesceeceececceececeaeeeceaeeeseeeesaueessaeeesaueessaeeeseaeessegeesseeeeseaeeesaaees 16 Chamber Temperature Control cccccccccccssceceececeeececeeeeececeeseueessaecesseeessaeeeseaseeseueessaeeessneeeseaeeesaaees 16 FON PS io MER REO ROLE T 17 Hear OM I OMe as eter teense E T a 17 DONTOTT U RE m R 18 5 6 How to enter control data cccccccccsscccseeceececeeeecueccueeceucecsueesaueesseeceeecaeeseueessueesaeessueeseueensaeessaeessaeess 21 5 7 How to USE SampleGuald anssi a E oe ei a ee eects eee 26 5 8 How to use the condenser se ecce cem prit ro a opto p En yx k pex aedand eu dui ex an sub vU ER Nuus Uy See Hk Ra huie UE CE FehE x E Use Ma sede
40. ol Channel 1 open a field make CoolHeat Enable Pressure mbar 100 the entry and press Chamber Temperature Control C Wait to cool to Z6 ENTER again to Rotor Speed High close the field Heat ff Option Elapsed Time Heat Off Elapsed Time HH MM 00 00 e During a run you End nf Run Option Elapsed Time access this screen End of Run Elapsed Time HH MM 00 00 by pressing the SETTINGS key Pressure Control Regime Dri Pure Final Controlled Pressure mbar 0 e You can edit any parameter during a GENEUAGC LTD run in this Way HELP e Toggle Run Data Tl to select ENTER to edit MENU ESC to exit Locked to protect Toggle Lock protection of Run Data p your data Please note that Main Menu order has changed It now reads Sample Holder Type Sample Volume Solvent Run Ho 91 Last Used Not yet used Last Changed Meu entry Sample Holder Type Run Data Locked SAMPLE HOLDER Next Hun Humber 0 for none Run Nane Select Sample Holder e When selected you Sample Holder Type are presented with sample Volume ml Hicrotitre Plate the SAMPLE 30 lvent Deep Well Plate Tube Vial Holder eee Ment SampleGuard Control Temperature C sampleGuard Control Channel Other e Use Cursor keys to CoolHeat Enable Pressure mbar move up and down Chamber Temperature Control C Rotor Speed High g e Press ENTER to Heat ff Option Elapsed Time select Heat ff Elapsed Time L HH HMH 00 00 End of Run Option Elapsed Time En
41. on 6 1 Routine checks The remainder of this section will address the less frequent system checks 7 1 How to clean the chamber As mentioned in section 6 2 Problem Prevention TFA creep can give rise to debris being deposited on the inside of the chamber and on the Quartz glasses Periodic inspection of the glasses the inside of the chamber and rotor is recommended to avoid an unacceptable build up of potential contaminants Excessive build up of debris on the pivoting faces of the sample swings and rotors can give rise to sample swings sticking in the out position and result in product loss Inspect the sample swings on a weekly basis for free movement and the inside of the chamber and glasses for build up of debris Clean with acetone taking care to avoid contact with the outside paintwork and accessories of the chamber Use the accepted Health and Safety precautions in using acetone in this manner Page 35 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems Seals and bearings will wear with use Over a period of time this will result in a gradual decline in performance of your system Lack of any maintenance at all will result in unscheduled breakdowns and costly down time Planned preventative service and maintenance will keep your system operating at peak performance Please refer to section 12 Useful Information for contact details Keep a weekly log of the time taken for your system to rea
42. our requirement and press ENTER again to close the field 04 4542 Issue 2 2 October 2008 Page 21 of 4 Genevac Mega 980 amp 1200 Max Evaporating Systems Run Data locked When set to Yes this setting protects the run data from inadvertent corruption Press the ENTER key to toggle between Yes and No Note that you will not be able to make amendments or additions if the data is locked Lock the data after completing the run entry Next Run Number This control enables you to link and loop runs in any sequence If you want to link runs list the number of the run you want to follow the run you are entering Repeat this process for any subsequent runs you would like to link You can also loop a run The limitation of how many runs you can link will be either the maximum run time of 99 hours 59 minutes or 99 different steps Linked and looped runs can always be interrupted by manually stopping the system Use the DOWN cursor key to move to the next field Enter the title of the run that should be no more than 20 characters long Use the DOWN cursor key to move to the next field Sample Holder Type Press ENTER to display the Select Sample Holder drop down menu Use the UP and DOWN cursor keys to select the option and press ENTER If OTHER is selected the description should be no more than 20 characters long Sample Volume Press ENTER and input the volume in mls Solvent Press ENTER to display the So
43. r Run profiles can also be linked to provide multi stage runs Up to 100 different profile steps can be linked and iterative loops can also be programmed With a maximum single run time of just below 100 hours the system is extremely flexible to meet every requirement dada zssauN 4 Scroll pump 04 4542 Issue 2 2 October 2008 Page 6 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 04 4542 Issue 2 2 October 2008 3 Scope of delivery and installation You will have purchased your system with or without the option of commissioning by Genevac personnel Reference will be made in the following notes to the installation procedures required to cover these options On delivery it is advisable to unpack your system at the point of receipt to ease the movement of the component parts to the point of use 3 1 Checking the delivery Check the contents of the delivery as soon as possible against the delivery note and notify Genevac Ltd immediately of any missing or damaged parts Refer to section 13 for contact details 3 2 Arranging commissioning If your system is to be delivered separately Genevac Ltd will contact you prior to the delivery to agree a date to commission your system 3 3 Training Commissioning will normally include training in the basic operation of the System Further in house training is recommended to fully exploit the flexibility of the system THE EVAPORATOR MUST N
44. re Is automatically set to an optimised figure for the recognised solvent mixture selected Page 16 of 4 Run Hn 91 Last Used 12 25 03703703 L ROTOR SPEED Run Data Locked Next Run Sumber QO for none Run Mame Sample Holder Tupe Lou Sample Volume ml High 30 l vent Lyophi lise select Lamp On Ratio SanpleGuard Control Temperature C SanpleGuard Control Channel CoolHeat Enable Pressure mbar Chamber Temperature Control C AUTO Cool to 32 Control at 20 Rotor Speed Low Heat Off Option Heat Flow Heat Off Threshold Not Applicable End of Run Option Not Applicable End of Run Threshold Not Applicable Pressure Control Regime Controlled Pressure Controlled Pressure mbar 10 GENEVAC LTD HELP TL to select ENTER to accept ESC to cancel Kotor Speed Low Hun Ho 91 Last Used 14 40 03 03 03 Last Changed 12 35 03703703 Hun Data Locked Ho Next Hun Number 0 for none Hun Mame Example sample Holder Type Deep Well Plate Sample Volume ml 0 70 50 vent EtAac Hex SanpleGuard Control Temperatur HEAT OFF OPTION SanpleGuard Control Channel CoolHeat Enable Pressure mbar Select option for turning off CoolHeat Chamber Temperature Control Kotor Speed Elapsed Time eat Dff Option sample Detection Heat Off Threshold End of Run Option End of Run Threshold Pressure Control Regime Controlled Pr
45. removed form the rotor e Ensure that all the clamps seals tubes cables and these instructions are retained together The system is provided with wheels for easy movement for access and service It is also provided with feet which should normally be screwed down to prevent excessive movement during operation If the load is 300g or more out of balance the vibration sensor will close the system down automatically If the feet are not screwed down this can occur with considerably lower out of balance loads Feet shown in the retracted position 04 4542 Issue 2 2 October 2008 Page 38 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems Temperature e he sample temperature exceeds the control limit e he Coolheat lamps will not switch on Pressure e The system will not reach full vacuum General e The system did not stop when expected it to e herunis taking far longer than expected e he system has shut down and have an imbalance alarm prompt 04 4542 Issue 2 2 October 2008 8 Fault Finding This section will describe potential errors and faults that may occur and lists their probable causes The symptoms are listed on the left hand side of the page and the source of solutions on the right Always check that you have called up the correct run from the Select Run screen Probable cause e he wrong control channel has been selected or the probes have been connected to th
46. ri Eana aAA ENEA E HMM UT A M RS IINE 43 13 LS SRM PUTO IE OUR e es 44 These instructions are subject to change without notice No part of these instructions may be reproduced in any form or be processed duplicated or distributed by electronic or optical means without the written permission of Genevac Limited All rights reserved Genevac Limited These operating instructions should be read before you use the Genevac Mega Evaporating System Keep them near the system for easy reference Your attention is drawn in particular to Section 4 Safety 04 4542 Issue 2 2 October 2008 Page 3 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems 04 4542 Issue 2 2 October 2008 NS Ln M dil 1 Introduction The Genevac Series ll range of evaporation systems are state of the art and represent a significant step forward in evaporation technology for the R amp D laboratory Drawing on extensive experience in the drug discovery field the Mega systems are designed to provide very high performance coupled with ease of use As you will discover the system is simple to set up easy to operate and very flexible The status of the system is displayed and controlled on a single keypad display module indicating the run time the vacuum rotor temperature and chamber temperature on digital displays Simple to use up down controls enable the ru
47. sly until the lid is sufficiently or fully opened BEFORE CLOSING THE LID ALWAYS ENSURE THAT ALL PERSONNEL AND OBJECTS LIKELY TO BE ENTRAPPED ARE CLEAR OF THE LID AND MATING SURFACES PARTICULARLY IN THE AREA OF THE HINGE To close the lid select CLOSED on the LID DIRECTION switch and press both OPERATING switches simultaneously until the lid is fully closed Note that the system will not operate if the lid is not fully closed 4 3 Limitations of use Your Mega Series evaporating system is unsuitable for use under the following circumstances e With strong mineral acids such as HCl and HBr at all concentrations unless specifically built to order e For use as a pressure vessel Page 9 of 44 33 PURIFICATION SOLIDO PHASE SYTHE J ALIGO SHTHESIS 3r SOLUTION PHASE 1 30 SOLUTION MASE Z EMATE 100 MAINTENANCE TEST Z SELECT Rut 5n 1 went WATER ACETONITRILE Tli DOM Deis WATER ARRON HYTROTTD DAF BAS AE THAHOL BENDA LTD nEHU START EDIT GRAPH CLEAR CORY UM Genevac Mega 980 amp 1200 Max Evaporating Systems OPEM Inr T Het iml i X 15 ol BEEF WE t ml DEEP HE 4 ILI MT iml 4 X 15 40 imi BEEP WE 4 Onl DEFROST FLUGH OPTIONS 04 4542 Issue 2 2 October 2008 5 Getting started The following notes describe the basic start up set up and run instructions for your Mega evaporating system 5 1 How to switch on the pump Switch the pump mains switch on
48. t s that you have ee selected from the menu appear in the bottom HELP left corner of the TL EHTEH to select deselect select Done when finished ESC to cancel screen EtAac Hex GENEVYAC LTD Select Done when finished Once your selection has been made you are now given a new option to Auto Program the remaining run parameters Hun No 91 Last Used 12 25 03703703 SOLVENT Hun Data Locked Acetonitrile ACH Next Run Number 0 for none Anmonium Hydroxide CNHSOH Run Name Chloroform TCE Press Y to accept Auto sample Holder Tupe Di Chloro Ethane DCE Program sample Volume ml p AUT FR GRAM Solvent thul Acetamide CDMAc Auto Programme If you have entered an SampleGuard Control Temp remaining parameters unrecognised solvent by sampleGuard Control Chan TLles HLo ol EtOH i CoolHeat Enable Pressure Acetate EtAc selecting ier irom the Chamber Temperature Cont se Hex solvent menu you will Rotor Speed Methanol MeOH not be presented with Heat Off Opti Tri Ethyl mi TEA ue Spon au eat ption ri Wl Amine Heat Off Elapsed Time HH MM IFA Program End of Run Option THF End of Run Elapsed Time CHH MM Toluene PhMe Mater HZ0 Pressure Control Regime BP 50 Final Controlled Pressure mbar BP 180 Other Clear I GENEVYAC LTD HELP TL EHTEH to select deselect select Done when finished ESC to cancel EtAc Hex Genevac and the ATEX Directive
49. the above would result in the costs of repairs being charged Consumable Items Sample Guard thermocouple probes Control fuses 04 4542 Issue 2 2 October 2008 Page 43 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems Genevac Limited The Sovereign Centre Farthing Road Ipswich IP1 5AP United Kingdom Sales and Service Hotlines Service Hotline 44 0 1473 243000 Sales Hotline 44 0 1473 240000 Fax 44 0 1473 461176 Email salesinfo genevac co uk Web site http www genevac com Genevac Inc SP Industries 815 State Route 208 Gardiner NY 12535 United States of America Sales and Service Hotline 1 845 267 2212 Fax 1 845 267 2212 Email atlas genevacusa com 04 4542 Issue 2 2 October 2008 13 Useful information If you need to contact Genevac for assistance use either the telephone or fax Hotlines given It will always help Genevac Service if you have the serial numbers at hand for the components of your system If you need to contact Genevac Sales for information on Service Contracts or products use the telephone or fax Hotlines given Alternatively Email or visit our web site Page 44 of 44
50. the lid selector to close and close the lid of the evaporator ensuring that no items will be entrapped by the lid Press the START key The system will indicate that the chamber is warming up This will take approximately twenty minutes When the chamber is up to temperature the system will start automatically With aluminium sample holders of this thermal capacity some time can be gained by preheating them in an oven to 40 C Page 30 of 44 Genevac Mega 980 amp 1200 Max Evaporating Systems Notes on probe position and channel choice Use channel 1 to control the sample holder or rotor temperature as this will always ensure that the sample temperature will never exceed the control temperature you set Outer tubes or wells in microtitre plates invariably dry more quickly than central ones This is because the central positions are surrounded by cold neighbours Choose central positions to be sure that all locations are dry or an outer position if you are unsure of how long a particular run will take The upper rotor has two diametrically situated holes for probes Alternatively use one of the holes in the sample holder as shown Always use the same channel as the control channel as once this is changed for a particular program it will remain so If you do change the control channel for a particular run this may cause confusion between different users and could give rise to product damage Periodica
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