KingFisher Flex Quick Reference Guide
Contents
1. internal memory The protocol consists of three steps binding washing and eluting To perform Demo_Flex96 the following items incl in the KingFisher Flex plastics Demo Package in the transport box are needed e Four 4 KingFisher Flex 96 plates for tip comb binding washing and elution solution Cat no 97002540 One 1 Thermo Scientific KingFisher Flex 96 tip comb for DW magnets Cat no 97002534 KingFisher Flex head for 96 Deep Well plate Cat no 24074430 Note A corresponding demo protocol for KingFisher Flex 24 deep well head Cat no 2407440 is also stored in the instrument s memory Demo_Flex24 For more information see the protocol details on the KingFisher Flex display Demo protocol description Step name Plate type Reagent volume Step parameters Bind KingFisher 96 150 ul water Bind time 30 sec magnetic beads Speed Medium Wash KingFisher 96 150 ul water Wash time 30 sec Speed Medium Fast Elute KingFisher 96 100 ul water Elution time 30 sec Speed Slow Release Beads Disposal plate for beads Bind Release time 5 sec Speed Fast 2011 Thermo Fisher Scientific Corporation All rights reserved BindIt is a trademark and KingFisher and Microtiter are registered trademarks of Thermo Fisher Scientific Inc and its subsidiaries All other trademarks and registered trademarks are the property of their respective holders All cross references refer to the Thermo Scientific KingFish2. Thermo Scientific KingFisher Flex Quick Reference Guide KingFisher Flex Installation Checklist Step Procedure Reference 1 Remove both the transport locks indicated by tags See other side 1 the tip comb holder transport lock and p 24 Fig 3 9 3 11 2 the heating block transport lock p 25 Fig 3 12 3 13 2 Attach the shield plate Use the two screws supplied p 29 Fig 3 17 3 20 3 Switch the KingFisher Flex ON p 26 4 Select the protocol Change_heatblock Press start Insert the heating block by pressing it evenly into place Press start to confirm the p 27 Fig 3 15 3 16 installation 5 To insert Thermo Scientific KingFisher Flex heads select the Change_magnet protocol Press start Insert the KingFisher Flex head so p 31 Fig 3 22 3 23 that the three pins slot into place Press start to confirm the installation 6 Run the check protocol Check_96dw_tip Check_24dw_tip Check _KF96_ p 35 tip or Check_pcr_tip according to the KingFisher Flex head and plastics you are using If the check run is all right proceed with your own runs A 2 i KingFisher Flex Brief User s Guide Step Procedure Reference 1 Switch the KingFisher Flex ON p 40 2 Pipette the samples reagents and wash buffers into the plates p 13 3 Combine the tip comb and Thermo Scientific KingFisher plate p 53 Fig 4 37 4 38 4 Select the desired protocol from the keypad or use Thermo Scientific Bindlt p 45 So
3. er Flex User Manual Rev 1 2 Cat no N07669 Manufactured by Distributed by Thermo Fisher Scientific Oy P O Box 100 Fl 01621 Vantaa Finland Tel 358 9 329 100 Fax 358 9 3291 0415 www thermo com 2 Rev 1 3 Cat no NO7730
4. ftware to run the protocol Use the USB or RS 232C cable to connect the instrument and PC 5 Press start Alternatively if the PC connection is used follow the p 45 94 instructions on the instrument display carefully 6 Open the sliding door if the see through lid is in place p 34 Fig 3 25 7 Load the plates onto the loading station according to the protocol Press p 59 Fig 4 38 4 40 start after each plate to confirm the loading 8 After the process remove all the plates according to the protocol Confirm removal by pressing start after each plate Note that the plate containing p 98 your samples is removed first 9 Finally END_OF_RUN is displayed At the end of the run press stop p 98 10 Switch OFF the KingFisher Flex p 60 1 Rev 1 3 Cat no N07730 SCIENTIFIC Processing volumes vs plate types and magnetic heads for KingFisher Flex Head Plate KingFisher 24 Microtiter 96 KingFisher 96 PCR plate Deep Well plate Deep Well plate plate skirted KingFisher Flex head for 200 5000 ul KF 24 Deep Well plate KingFisher Flex head for 50 1000 ul 50 150 ul Microtiter 96 Deep Well plate KingFisher Flex head for KF 96 plate 20 200 ul KingFisher Flex head for PCR plate 20 200 ul 20 100 ul Demo protocols Demo_Flex96 is a short and simple protocol for demonstrating 1 Tip comb holder KingFisher Flex operation The protocol is stored in the instrument s transport lock
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