RUO Data Sheet for International
Contents
1. Cytomegalovirus CMV Concentrated and Prediluted Cocktail Antibody Control Number 902 118 040215 Catalog Number CM 118A B C PM 118 AA Description 0 1 0 5 1 0 ml concentrated 6 0 ml prediluted Dilution 1 50 1 100 Ready to use Diluent Van Gogh Yellow N A Intended Use For Research Use Only Not for use in diagnostic procedures Summary and Explanation This antibody is a mixture of two monoclonal antibodies that reacts with immediate This antibody does not react with herpes virus or human papilloma virus In the later stage early and early protein antigens in tissues infected with cytomegalovirus of infection a cytoplasmic reaction may be observed Principle of Procedure Antigen detection in tissues and cells is a multi step immunohistochemical process The initial step binds the primary antibody to its specific epitope A secondary antibody may be applied to bind the primary antibody followed by an enzyme labeled polymer or an enzyme labeled polymer may be applied directly to bind the primary antibody The detection of the bound primary antibody is evidenced by an enzyme mediated colorimetric reaction Source Mouse monoclonal Species Reactivity Human others not tested Clone DT10 BC90 Isotype IgG2a IgG1 Total Protein Concentration 10 mg ml Call for lot specific Ig concentration Epitope Antigen Cytomegalovirus proteins Cellular Localization Nuclear staining pattern with some cytoplasmic stain2. 22 Atlanta GA April 30 1976 Decontamination of Laboratory Sink Drains to Remove Azide Salts 3 Clinical and Laboratory Standards Institute CLSI Protection of Laboratory Workers from Occupationally Acquired Infections Approved Guideline Fourth Edition CLSI document M29 A4 Wayne PA 2014 Page 1 of 1 Fax 925 603 8080
3. act with lead and copper plumbing to form highly explosive metal azides Upon disposal flush with large volumes of water to prevent azide build up in plumbing Center for Disease Control 1976 National Institute of Occupational Safety and Health 1976 2 2 Specimens before and after fixation and all materials exposed to them should be handled as if capable of transmitting infection and disposed of with proper precautions Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens If reagents or specimens come in contact with sensitive areas wash with copious amounts of water 3 3 Microbial contamination of reagents may result in an increase in nonspecific staining 4 Incubation times or temperatures other than those specified may give erroneous results The user must validate any such change 5 Do not use reagent after the expiration date printed on the vial 6 The SDS is available upon request and is located at http biocare net Technical Support Contact Biocare s Technical Support at 1 800 542 2002 for questions regarding this product References 1 Vago L et al Coinfection of the central nervous system by cytomegalovirus and herpes simplex virus type 1 or 2 in AIDS patients autopsy study on 82 cases by immunohistochemistry and polymerase chain reaction Acta Neuropathol 1996 Oct 92 4 404 8 2 Center for Disease Control Manual Guide Safety Management NO CDC
4. ing Positive Control CMV infected tissues Known Applications Immunohistochemistry formalin fixed paraffin embedded tissues Supplied As Buffer with protein carrier and preservative Storage and Stability Store at 2 C to 8 C Do not use after expiration date printed on vial If reagents are stored under conditions other than those specified in the package insert they must be verified by the user Diluted reagents should be used promptly any remaining reagent should be stored at 2 C to 8 C Staining Protocol Recommendations manual use Peroxide Block Block for 5 minutes with Biocare s Peroxidazed 1 Pretreatment Solution recommended N A Pretreatment Protocol N A Digestion Method Digest with Pepsin enzyme for 5 minutes at 37 C or for 15 minutes at room temperature Protein Block Optional Incubate for 5 10 minutes at RT with Biocare s Background Punisher Primary Antibody Incubate for 30 minutes at RT Probe Incubate for 10 minutes at RT with a secondary probe Polymer Incubate for 10 minutes at RT with a tertiary polymer Chromogen Incubate for 5 minutes at RT with Biocare s DAB OR Incubate for 5 7 minutes at RT with Biocare s Warp Red Counterstain Counterstain with hematoxylin Rinse with deionized water Apply Tacha s Bluing Solution for 1 minute Rinse with deionized water Biocare Medical LLC 4040 Pike Lane Concord CA 94520 coal Tel 800 799 9499 www biocare net OAR 118 T60 60 tes
5. ts prediluted Ready to use N A Protocol Recommendations ONCORE Automated Slide Staining System OARI18 is intended for use with the ONCORE Automated Slide Staining System Refer to the ONCORE Automated Slide Staining System User Manual for specific instructions on its use Protocol parameters in the ONCORE Automated Slide Stainer Protocol Editor should be programmed as follows Protocol Name CMV Protocol Template Description Ms HRP Template 1 Dewaxing DS Option DS Enzyme Antigen Retrieval AR Option AR2 low pH 80 C Reagent Name Time Temp CMV 30 min 25 C Technical Note This antibody has been optimized for use with Biocare s MACH 4 Universal HRP Polymer Detection and ONCORE HRP Detection Other Biocare polymer detection kits may be used however users must validate incubation times and protocols for their specific application Use TBS for washing steps Limitations This product is provided for Research Use Only RUO and is not for use in diagnostic procedures Suitability for specific applications may vary and it is the responsibility of the end user to determine the appropriate application for its use Precautions 1 This antibody contains less than 0 1 sodium azide Concentrations less than 0 1 are not reportable hazardous materials according to U S 29 CFR 1910 1200 OSHA Hazard communication and EC Directive 91 155 EC Sodium azide NaN used as a preservative is toxic if ingested Sodium azide may re
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