FastPrep® Cell Disrupter INSTRUCTION MANUAL Models FP100
Contents
1. Thermo Savant FastPrep Cell Disrupter INSTRUCTION MANUAL Models FP100 FP120 FP220 FP100A FP120A and FP220A Qbiogene Inc North America Qbiogene Inc Europe 2251 Rutherford Road Parc d Innovation BP72 Carlsbad CA 92008 67042 Illkirch Phone 800 424 6101 Cedex France Fax 760 918 9313 Phone 33 0 388 675 425 Fax 33 0 388 671 945 143 3000 00 Rev D 2002 Thermo Savant A Thermo Electron business Section 1 0 Section 2 0 Section Section 4 0 Section 5 0 Section Appendix 1 Appendix 2 Appendix 3 FastPrep is a registered trademark of Qbiogene This purchase of this machine grants to the purchaser the right to practice the process described in the United States Patent 5567050 and 5643767 2002 Thermo Savant Table of C ontents Section Title Introduction Introduction Section Title Installation Installation Unpacking Inspection Set up Connecting the Power Section Title Operation Operating Instructions Preparing the FastPrep for Operation Control Panel Programming a Run Loading and Securing the Samples For Models FP100A FP120A and FP220A For Models FP100 FP120 and FP220 Running the Unit Section Title Specifications Specifications Section Title Warranty and Liability Warranty amp Liability Section Title Appendices Maintenance amp Cleaning An Explanation of FastPrep Instrument Speed Settings Emergency Interl2. SPEED TIME COVER is used to select either SPEED or TIME to be shown in the display Press cover to release the lid Note Run settings cannot be changed once the run is in progress 3 3 3 PROGRAMMING A RUN Note For application protocols refer to extraction kit instructions supplied by Qbiogene A Select desired speed The units displayed are in meters second See Appendix 2 for explanation of FastPrep Instrument speed settings Set switch A SPEED TIME COVER to SPEED position Press and hold CHANGE on switch B RUN OFF CHANGE to scroll to the desired speed setting When the switch is released it returns to the OFF position Note While setting run speed the display counts forward in increments of 0 5 m sec within the operating range of 4 0 6 5 m sec B Select desired time of run The units displayed are seconds Set switch A SPEED TIME COVER to TIME position Press and hold CHANGE on switch B RUN OFF CHANGE until the desired time setting is shown on the display The longer CHANGE is held the faster the incre ments are displayed When the switch is released it returns to the OFF position Note While setting run time the display counts forward in increments of 1 second within the operating range of 1 45 seconds 3 4 LOADING AND SECURING THE SAMPLES 3 4 1 FOR MODELS FP100A FP120A and FP220A The main power must be ON to open the FastPrep cover Unlock and open cover by setting switch A SPEED TI
3. be damage report it to the carrier and contact Qbiogene immediately Save the packaging material in the event a return is necessary Set up To assure safe operation and best results read this manual in its entire ty before operating the FastPrep Instrument The FastPrep Instrument comes fully assembled requiring very little set up Install the system on a clean dry level stable surface within 4 feet 1 2m of a compatible electrical outlet Allow 1 2 inches 3 5 cm of space around the FastPrep Instrument for proper motor ventilation This unit is FOR INDOOR USE ONLY Avoid operating in areas of excessive humidity or extremes of temperature IMPORTANT Remove motor locking plate prior to use as per instructions See on following page WARNING The hold down bracket must be removed before operating the FastPrep Instrument HOLD DOWN BRACKET SCREW CHAMBER COVER PLATE To Remove the hold down bracket Loosen the two screws Slide the bracket out and discard Retighten the two screws Your unit is now ready for operation 2 0 3 0 3 1 3 2 INSTALLATION cont d Connecting the power WARNING Before connecting the FastPrep Instrument to an outlet make sure that the voltage frequency and amperage are compatible with the requirements indicated on the label on the instrument rear panel If there are any questions please consult an electrician Make sure the power switch loc
4. ME COVER to COVER position Loosen the three clamps that secure the spoke plate by lifting up on the clamp levers as shown in Figure 2 Note the anti rotation pin located under each latch arm Using the rotation knob rotate the spoke plate clockwise to expose the sample cav ities WARNING Before inserting sample tubes always make sure that tube screw caps are tight Load sample tubes into the sample holder Whenever possible position the tubes symmetrically Make certain that the tubes are fully seated into the sample holder The FastPrep Instrument is designed to be used exclusively with the FastPrep kits and reagents These can be purchased from Qbiogene at 800 424 6101 North America or 33 0 388 675 425 Europe Use of non approved reagents may result in damage to the FastPrep Instrument leakage of content and instrument contamination Also unapproved reagents may cause sample tubes to burst in downstream processing Anti rotation pin Pin well Latches Closed 5 When all samples are loaded rotate the spoke plate by turning the rotation knob counterclockwise so that the retention spokes are centered above each sample tube cap Press down on the clamp levels to secure as shown in Figure 3 making sure the anti rotation tab is inserted down into the spoke plate WARNING Samples must be secured properly before running the FastPrep Instrument Close and lock cover of FastPrep 3 4 LOADING AND SECU
5. RGENCY INTERLOCK OVERRIDE If a general electrical power failure should occur while the FastPrep Instrument is in use the cover will remain locked The cover may also remain locked if an error code appears in the display In such an emergency access to samples may be gained by means of the emergency interlock override 1 Unplug power from electrical unit 2 Release vacuum under suction cup feet 3 Slide the FastPrep Instrument forward until the emergency interlock override handle is visible underneath situated toward the front The override mechanism is a flat metal handle with release cord wound around attached to the undersurface by two Phillips head screws 4 Remove the screws 5 Holding the metal handle pull down on cord until the safety interlock releases There will be an audible click and the cord will release 6 Remove samples 7 Rewind release cord around handle 8 Replace screws reattaching handle to undersurface of the FastPrep Instrument 9 Slide the FastPrep Instrument back to its previous location 10 Reconnect power cord 12 143 3000 00 Rev D 2002 Thermo Savant
6. RING THE SAMPLES 3 4 2 FO Spoke Sample holder Sample cavity S R MODELS FP100 FP120 and FP220 The main power must be ON to open the FastPrep cover Unlock and open cover by setting switch A SPEED TIME COVER to COVER position Loosen the three knobs that secure the spoke plate by turning the knobs counter clockwise see Figure 2 CAUTION Do not completely remove these screws Using the rotation knob rotate the spoke plate clockwise to expose the sample cav ities WARNING Before inserting sample tubes always make sure that their screw caps are tight Load sample tubes into the sample holder Whenever possible position the tubes symmetrically Make certain that the tubes are fully seated into the sample holder The FastPrep Instrument is designed to be used exclusively with the FastPrep kits and reagents These can be purchased from Qbiogene at 800 424 6101 North America or 33 0 388 675 425 Europe Use of non approved reagents may result in damage to the FastPrep Instrument leakage of content and instrument contamination Also unapproved reagents may cause sample tubes to burst in downstream processing Retention spoke plate Rotation ecuring knob knobs Open position Closed position Figure 2 FastPrep FP120 sample holder showing both open and closed positions When all samples are loaded rotate the spoke plate by turning the rotation knob counterclockwise so that the r
7. UN OFF CHANGE to OFF to unlock the cover the EE display stays on for 2 seconds then returns to the preset value Open cover and remove sample tubes Warning Use caution when removing samples sample holder may become hot after the run CAUTION To prevent sample overheating machine must rest for 5 10 min utes between runs SPECIFICATIONS Controls Time Speed Acceleration Deceleration Duty Cycle Construction Dimensions W x D x H Weight Power Requirements Safety Registration Programmable run time and speed large LED readout Range 1 45 sec Programmable in 1 sec increments Range 4 0 6 5m sec Programmable in 0 5 m sec increments lt 2 seconds to maximum speed lt 2 seconds to stop 6 5 m s for 45 seconds with a 5 minute rest period between runs Anodized aluminum sample holder Polypropylene sample retention spoke plate Chemical resistant painted surfaces 11 in x 15 75 in x 12 in 28 0 cm x 40 0 cm x 30 5 cm 36 lbs 16 3 kg 100 V AC 50 60 Hz 8 A JAPAN ONLY 120 V AC 50 60 Hz 8A 230 V AC 50 50 Hz 5 A CSA and CE Approved ISO 9001 1994 Registered by QMI Quality Management Institute Certificate number 001080 5 0 WARRANTY amp LIABILITY The FastPrep Instrument product is warranted against defects in material and work manship for one year after the date of delivery to the original purchaser This war ranty is limited to defective mate
8. ated on the rear panel is OFF Connect the power cord to the instrument power fixture is on the rear panel and plug it into a com patible outlet Note A safety latch prevents the FastPrep lid from opening when the main power switch located on the rear panel is OFF Before switching power ON make sure that the unit is properly set up connected to a suitable power source and the RUN OFF CHANGE switch on the front control panel is turned OFF see Figure 1 Below Once the main power is ON press COVER SPEED TIME COVER switch to release the lid OPERATING INSTRUCTIONS PREPARING THE FASTPREP FOR OPERATION Turn the main power switch ON This switch is located on the rear panel of the instru ment When switched ON factory set default values automatically program FastPrep to operate at speed 4 0 m sec and run time 20 seconds to override these values see section 3 3 below CONTROL PANEL A DISPLAY B SPEED 70 RUN TME OFF COVER CHANGE Figure 1 FastPrep FP120 FP120A Control Panel There are two rocker switches on the instrument front panel Figure 1 Switch B RUN OFF CHANGE allows the user to choose RUN for operation cellular disrup tion or CHANGE to program the desired operating parameters speed or time Setting the switch to the OFF center position opens the lid after a run is complet ed The OFF position can also be used if necessary to terminate a run that is in progress Switch A
9. ed under this warranty 10 APPENDIX 1 MAINTENANCE amp CLEANING Maintenance The FastPrep Instrument requires no scheduled maintenance Clean surfaces immediately after contact with sample solutions or reagents Cleaning The FastPrep Instrument should be cleaned if reagents or sample solu tions spill on or inside the unit If a sample tube leaks during a run the solution will be sprayed on the inside cover Always clean up any spray or spills immediately using a damp paper towel Always wear gloves and protective clothing when cleaning The components of the FastPrep Instrument are chemically resistant and com patible with most cleaning solutions The spoke plate can be removed for a thorough cleaning To remove the spoke plate unscrew and remove the three knobs by gently pulling up on the knobs and turning counterclockwise until they come completely free from the unit Carefully remove the three knobs remove the spoke plate and then remove the three wash ers and three springs under each knob Clean spoke plate with damp cloth or paper towel Reassemble instrument in reverse order If potentially infectious agents are used in the FastPrep Instrument spills should be cleaned immediately and appropriate decontamination carried out The FastPrep Instrument may be not be resistant to all cleaning regi mens required for all infectious agents Exercise appropriate caution and wear protective clothing eyewear and gloves whe
10. etention spokes are centered above each sample tube cap Hand tighten the three securing knobs while pushing down on the rotation knob WARNING Samples must be secured properly before running the FastPrep Instrument Overtightening of FastPrep knobs may result in damage to FastPrep spokeplate and FastPrep knobs Close and lock cover of FastPrep 3 6 RUNNING THE UNIT Start run by setting switch B RUN OFF CHANGE to RUN If display flashes CC the cover is not properly secured Firmly press down cover to lock As a safety feature the FastPrep Instrument will not operate when cover is not properly locked To display speed during the run set switch A SGPEED TIME COVER to SPEED To display time remaining in the run set switch A GPEED TIME COVER to TIME Note When SPEED is selected on switch A AA is displayed during motor acceleration When the motor reaches its preset speed the set speed value is dis played If the set speed is not reached within 10 seconds of starting a run an error message appears E 3 and the run is automatically aborted During motor decel eration at the end of a run dd is displayed If the motor does not decelerate to a complete stop within 10 seconds error message E 4 appears Call Qhiogene immediately if an error code appears At the end of a run the motor brakes to a stop automatically and the display reads EE to indicate that the run has ended Set switch B R
11. n working with potentially infectious samples Contaminated units should be kept in an appropriate biosafety level facility and should only be maintained or serviced by per sonnel trained in safe handling practices specific to the infectious agent APPENDIX 2 AN EXPLANATION OF FASTPREP INSTRUMENT SPEED SETTINGS The cell disruption process during a FastPrep Instrument run is caused by the col lision of matrix and sample within the FastPrep Instrument sample tube The rate of collision and energy of impact both of which determine the effectiveness of the disruption process are a function of the FastPrep Instrument speed settings and specific gravity of the bead material used The FastPrep Instrument speed settings in meters per second refer to the maximum vertical velocity achieved by a sample tube during reciprocating motion The rate of collision is proportional to speed while the energy of impact is proportional to the square of the speed For example a 50 increase in the FastPrep Instrument speed setting increases the rate of col lision by 50 and at the same time increases the energy of impact by 125 The FastPrep Instrument has been specifically designed to allow operation within an ideal range of parameters for disrupting membranes from a wide variety of cell types When used with cell specific protocols and kits Qbiogene www qbio gene com cell membrane disruption and nucleic acid yield is maximized APPENDIX 3 EME
12. ock Override Page Page Ww UY W W Fe 1 Page OO N ou A UW W W I 1 co Page Page 10 Page 11 11 12 1 0 INTRODUCTION The FastPrep is a high speed benchtop reciprocating device for efficient dis ruption of cell membranes The unit is designed for use with the FastPrep Extraction Kits Qbiogene www qbiogene com These kits are available for a wide variety of extraction and purification applications The FastPrep sample holder has the capacity to process 12 FastPrep sample tubes simultaneously We do not recommend using any tubes other than those supplied with the FastPrep Kits The front control panel of the FastPrep Instrument includes a digital display and two control switches Speed 4 0 6 5 m sec and run time 1 45 seconds are fully programmable The microprocessor measures and displays time and speed during each programmed run For information on FastPrep kits other kits for use with the FastPrep Instrument and their various applications call Qbiogene at 800 424 6101 North America or 33 0 388 675 425 Europe or visit the FastPrep Users Resource Group at http www qbiogene com 2 0 INSTALLATION Unpacking Carefully remove the FastPrep Instrument and accessories from the shipping carton Compare the packing list to the box contents If there is a discrepancy call Qbiogene Inc Inspection Inspect the unit for any damage that may have occurred during shipment Should there
13. rials and workmanship and does not cover inciden tal or consequential damages Qbiogene will repair free of charge any apparatus covered by this warranty If a new component fails to work we will replace it absorb all charges and continue the one year warranty period Warranty work is subject to our inspection of the unit No instru ments equipment or accessories will be accepted without a Return Material Authorization RMA number issued by Qbiogene Costs of shipping the unit are not covered under warranty The warranty obliges you to follow the precautions in this manual When returning apparatus that may contain hazardous and or infectious materials you must pack and label them according to US Department of Transportation DOT and or European Community EC regulations applying to transportation of haz ardous and or infectious materials Your shipping documents must also meet DOT and or EC regulations All returned units must be fully decontaminated of any chemical biological or infectious agent Use of this equipment in manners other than those specified in this manual may jeop ardize personal safety Under no circumstances shall Qbiogene or Thermo Savant be liable for damages due to the improper handling abuse or unauthorized repair of their products Qbiogene and Thermo Savant assumes no liability express or implied for use of this equipment Use of non approved kits and reagents with the FastPrep Instrument is not cover
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