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1. Front view EVE Automatic Cell Counter 2013 NanoEnTek Inc Rear and side view Power inlet Connect the counter to an electrical outlet using the supplied power cord and the appropriate plug based on the electrical outlet configuration in your country Image adjustment focus knob The image adjustment focus knob is used to adjust the image quality to obtain better contrast between live bright centers and dead dark blue centers cells This is important to obtain accurate cell counts and viability measurements Focus lock knob Power inlet The focus lock knob is used to lock the image adjustment focus knob once the image is optimized There is no need to use the focus lock knob but is available for convenience if you are measuring multiple samples of the same cell type Focus lock Focus knob Rear view Side view Installation Installing EVE Automatic cell counter 1 After unpacking the instrument place the instrument on a flat level dry surface 2 Plug one end of the supplied power cord into the EVE To the other end attach the appropriate plug adaptor based on the electrical outlet configuration in your country 3 Plug the power cord into the electrical outlet Be sure to use only the power cord supplied with your instrument Powering the instrument with an unapproved power cord may damage the instrument 4 When you are ready to us
2. This product conforms to UL61010 1 CSA C22 2 No 61010 1 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part l General Requirements Instruments bearing the TUV symbol are certified by TUV Product Services to be in conformance with the applicable safety standards for the US and Canada EVE Automatic Cell Counter 2013 NanoEnTek Inc 39 Product specifications Environmental conditions Operating power AC 100 240 VAC 1 5A Frequency 50 60 Hz Electrical input 12 VDC 1 0 A Installation site Indoor use only Operating temperature 5 40 C Maximum relative Humidity 20 80 Altitude lt 2 000 m Transient category Installation categories II Pollution degree 2 Degree of protection IPXO EVE instrument Instrument type Benchtop cell counter Counting time lt 20 seconds Cell measurement range cells mL 1 x 10 1 x 10 Optimal measurement range cells mL 1 x 10 4 x 10 Cell size range 5 60 um Dimensions 27 cm W x 20 cm D x 19 cm H Weight 2 1 kg EVE Cell counting slide Material Polymethy methacrylate Dimensions 75 mm L x 25 mm W x 1 8 mm H Chamber depth 100 um Chamber volume 10 uL EVE USB drive 2 GB 40 Ordering information The following products can be used with the EVETM and are available separately from NanoEnTek
3. Cat No Description Contents EVS 050 EVE Cell counting slide 50 slides 100 counts with 1 ea X 1 5 mL of trypan blue 0 4 EVS 1000 EVE Cell counting slide 1 000 slides 2 000 counts with 20 ea X 1 5 mL of trypan blue 0 4 EVS 5000 EVE Cell counting slide 5 000 slides 10 000 counts with 100 ea X 1 5 mL of trypan blue 0 4 EBB 001 Test beads 2eaX1 mL Concentration avg 1 0 X 10 EVE Automatic Cell Counter 2013 NanoEnTek Inc 41 Technical Support Visit the our Website at www nanoentek com for Technical resources including manuals FAQs etc Technical support contact information Additional product information and special offers For more information or technical assistance please call or email NanoEnTek Inc HQ 12F Ace High end Tower 235 2 Guro3 dong Guro gu Seoul 152 740 Korea Tel 82 2 6220 7913 Fax 82 2 6220 7721 NanoEnTek USA Inc 5627 Stoneridge Drive Suite 304 Pleasanton CA 94588 USA Tel 1 925 225 0108 Fax 1 925 225 0109 Email sales nanoentek com Website www nanoentek com 42 All the materials in this user guide are protected by Korean and international copyright laws They cannot be reproduced translated published or distributed without the permission of the copyright owner EVE User manual Website www nanoentek com E mail sales nanoentek com l Manufactured by NanoEnTek Inc 12F Ace High e
4. Automatic Cell Counter 2013 NanoEnTek Inc 93 Troubleshooting Inaccurate cell count Sample handling Do not insert the EVE Cell counting slide upside down as this may introduce liquid into the instrument that could damage it Do not reuse the EVE Cell counting slides as leftover dye from the previous reading may affect the next reading Do not use any other counting slides such as a glass hemocytometer with the EVE as it results in inaccurate cell count and may damage the instrument Ensure that the sample covers the entire counting area and the EVE Cell counting slide is inserted completely into the counter Low and high readings The EVE is designed to read samples from 1 x 10 cells mL to 1 x 10 cells mL with the highest accuracy between 1 x 10 cells mL and 4 x 10 cells mL If your sample is not in this range you may need to dilute the sample or add more cells and read the sample again Poor image quality While viewing cells in the Zoom mode use the focus knob to adjust the image to ensure that live cells have bright centers and dead cells have dark blue centers Cell clumping Error codes Ensure the cells are not clumped To maintain instrument sensitivity we recommend that you calibrate the counter each year as described on page 23 See page 36 for a description of error codes 34 Saving and printing problems Use the USB drive
5. between large or small sizes Measures cell concentrations ranging from 1 x 10 to 1 x 10 cells mL and cells with sizes ranging from 5 um to 60 um Provide the clumping cell counting function to get more accurate results using EVE PC software Uses disposable cell counting slides that eliminate washing steps and cross contamination between samples Saves and print cell count data including images using the EVE software and EVE USB drive Presents comprehensive data with graphical reports and as a CSV comma separated value file for sample comparisons Description Front view Power button The power button is used to turn the instrument on and off The red status light indicates that the instrument is off the green status light indicates that the instrument is on LCD touch screen Located in the front of the instrument contains buttons for all the functions needed and displays data from the cell count Slide port The slide port is used to insert the EVE Cell counting slide containing the sample with trypan blue stain into the counter for analysis USB port Power button Slide port USB port LCD touch screen The USB port allows you to transfer and save the cell count data and image to your computer for record keeping and printing purposes The USB drive supplied with the instrument or any other standard USB drive is inserted into the USB port for data transfer
6. button to make the changes To restore default parameters press Default button 3 Press Save protocol button to create a new protocol EVE Automatic Cell Counter 2013 NanoEnTek Inc 4 Type protocol name and user name in the appropriate fields and press Save button again 5 Once a protocol is saved it is available for use at any time Press Load protocol button 6 The protocol appears in the protocol menu Use the up and down arrows to find your saved protocol To use the protocol select one of protocol and press Apply button Protocol User 1 DEFALI KHNA Y 2 PTI_SAMPLE ANI 3 PT2 PROTOOL PETER 4 5 press Delete button to delete protocol press Edit button to edit protocol TM Date and 1 Turn on the EVE by pressing the Power button The Start i up screen is displayed after a few seconds time setup 2 Press Settings and then press Date and Time 3 The Date Time Properties screen is displayed To select the month and day scroll to the appropriate month using the arrow keys and then press the day on the calendar Use a pointed object like a stylus or pipette tip to push the small buttons on the calendar 4 To select the time scroll to the appropriate time and select Automatically adjust clock for daylight saving if needed Press Apply or OK button to make the date time changes The updated date time is displayed on the top of the window Once
7. mix well Mix gently by pipetting up and down 4 Load 10 uL of the sample mixture on EVE Cell counting slide side A using pipette The two chambers of the slide are labeled A and B for easy tracking of your samples a EVE Automatic Cell Counter 2013 NanoEnTek Inc 15 ounting 5 Insert the EVE Cell counting slide sample side slide A first into the slide port on the instrument you will hear a soft click if the slide is pushed in correctly Each chamber is counted separately 6 Press the Count cells or Next sample button 7 Adjust the image by pressing the Zoom button Navigate by pressing the location you like to see on the grid 8 While viewing cells in the zoom mode use the Focus knob to adjust the image After you have counted the first sample you may not have to use the Focus knob again If measuring multiple samples of the same approximate size you may lock the Focus knob You are able to unlock the knob later to adjust the image Operation Cell counting Optimize the image for analysis such that Live cells have bright centers and dark edges Dead cells have a uniform blue color throughout the cell with no bright centers lt Correct Image gt lt lncorrect Image 1 gt lt lncorrect Image 2 gt Dead cells have bright Live cells have dark centers and are blue centers are counted as live counted as dead EVE Automatic Cell
8. of ve ONA S 205 ery ch wl EVE Operation Seivane v2 06 on itis mcommended that you GRE all other programs balire preteed wits ind lo Click Ngxi to continua Installation Oy click lance to cancel installation Nest Cancel 3 If you want to change installation folder click Browse and choose the location that you want And click Install 5 a instalation of EVE Operation Software v2 05 i Select install D ol Becwpe For Folder HE t La ld Sofware v S will be installed ta Selec ingtall tolder Click lestall gt t begin ixetalliHen 2 tllowing for me en oh v a ier k paan Eh ane ns path de ticie Space needed l 34FElBiyles Insiall Ipk3gr he Program Files Brewaa i Pragram Files gabi ng i Cancel EVE Automatic Cell Counter 2013 NanoEnTek Inc 4 The computer activates the installation of the Software fl gt 2talfatlon of EVE Operation sofware v2 08 installing EVE Osrgdion Sofware v E Ciki Abort t abort instaladon InetMliag bae CA Pregam Flest Evi InstMling Bem agurgarri gi Progress Abort 5 Click OK when Installation is complete i inizallation of EVE Operation fottware v2 05 x heatalaton of EVE Dperation sotaan v0 bimini 6 When the software is installed the PC software icon appears on your desktop 7 Click on the PC software icon on your PC St
9. supplied with the counter or an USB 2 0 drive as some types of USB drive are not detected or recorded by the counter Incorrect USB drive Do not save too many files in a USB drive as the counter may slow down to read the USB drive Do not remove the USB drive or turn off the counter when updating Accidentally removed the USB drive Do not remove a USB drive when saving or reading data as it may damage the counter Instrument not updating firmware Download a new version of the firmware from the website May be using a corrupted on a different USB drive and try updating the firmware on firmware file or a damaged the EVE Contact technical support if the problem persists USB drive EVE Automatic Cell Counter 2013 NanoEnTek Inc 35 Error codes This section describes the error codes displayed by the EVE when it encounters a problem Contact technical support page 42 for details on error codes and if you need to send the instrument back for servicing Position Driver Preview Memory Reason Message Camera driver loading fail Camera driver open fail Camera driver initializing fail System Error E0101 GPIO driver loading fail Reboot the device or refer to service personnel Camera preview fail Image buffer memory allocation fail Camera memory allocation fail Error code 0101 0102 0103 0104 0201 0301 0302 36 Warranty Nanoen
10. them 30 Spread sheet 1 Select the saved file you wish to open and click Spread sheet button Atomitie ctl counter 2 User can check cell distribution according to cell size differences And also this information can be exported by EVE Automatic Cell Counter 2013 NanoEnTek Inc 31 Prepare 1 Select the saved file you wish to open and click Prepare report button Automatic cel counter n co g Report report 2 You can check preview of report before export to PDF file 3 Click Export to PDF button to obtain a printable version of the image and data Export to POF _ 4 Type the file name and select location for saving file mavr As Becent Diesk hog My Documents 5 You can check the file on the location you selected 32 Cleaning amp maintenance Cleaning and Clean the surface of the EVETM with a damp cloth To clean the LCD screen turn off the EVE disconnect the power cable and clean the LCD screen with a soft cloth lightly moistened with LCD cleansing detergent Cleaning the screen with excessive force can damage the LCD the screen Wipe the screen dry immediately Do not reuse the cell counting slides maintenance The EVE does not need regular maintenance To troubleshoot problems with EVE contact technical support page 42 Do not perform any repairs or service on the EVE to avoid damaging the instrument EVE
11. C EVE Automatic cell counter User Manual NanoEnTek Table of contents Product contents 4 Product overview 5 6 Description 7 8 Installation 9 Operation 10 24 Settings 10 Cell mode parameters 10 12 Date and time setup 13 Recommend actions Cell counting 15 19 Transferring data to a computer 20 Using the beads mode for counting beads 21 22 Calibrating 23 24 PC software 25 32 Installation 25 26 Open the data 27 28 Cell gating 29 Counting 30 Spread sheet 31 Prepare report 32 Cleaning and maintenance 33 Troubleshooting 34 35 Error codes 36 Warranty 37 Safety precautions 38 39 Product specifications 40 Ordering information 41 Technical support 42 EVE Automatic Cell Counter 2013 NanoEnTek Inc 5 Product contents EVE is shipped with the following components Please check that all items listed below were shipped receiving the instrument If any items are missing or damaged contact your local distributor or e mail sales nanoentek com Mo BE k N k Automatic cell counter Power cord with 4 adaptor Cell counting slides cords 4 pcs set for U S Canada Taiwan Japan Europe or UK with 1 5 mL of Trypan blue 0 4 1 EA 1 SET 1 BOX 50 slides box Cat No Cat No EVE MC EVS 050 E Z o _ USB drive User manual Quick manual 2GB 1EA 1EA 1 EA Quick Manual PPP 3 53 AufomaSc cal courier Product overview The EVE Automatic
12. Counter 2013 NanoEnTek Inc 17 9 When you are satisfied with the image press Count Cells 10 After 20 seconds to count each sample and the cell count for live dead and total cells as well as percentage viability is displayed on the screen Record the cell count or insert a USB drive and press Save button see page 20 11 To see more details on the data as well as graphical representation of the data press the More data button ab z Number i 40 Coa atte jan Press the Close button to return to the main screen 18 12 The Calculator button allows you to quickly calculate adjustments to the cell suspension to obtain a desired concentration 13 To count the cells in the other side of the slide side B remove the EVE slide after side A is counted by pushing in the slide slightly and then pulling the slide out Turn the slide around and reinsert into the slide inlet and repeat the procedure 14 The EVE USB drive holds one set of data To save your data for future analysis or archiving you must record the data or save after each reading See page 20 for Transferring data to a PC 15 After recording or saving the data remove and discard the slide appropriately as bio hazardous waste 16 At this point the EVE is ready for another sample If you are not using the instrument press the Power button to turn off the instrument If the touch screen is not respondin
13. art up screen will be shown Automatic cell counter 26 1 Insert the USB to USB port of computer And Click Open button to load data files transferred from the instrument 2 Select the file that need to adjust counting result in different condition Open the data 3 Adjust the cell image by pressing the Zoom button Navigate the fields by clicking the location you like to see on the grid 4 If itis necessary you can adjust cell counting result using Right button of mouse interface You can change the circle mark recognized by counting algorithm of each cell to live or dead And also each cell can be deleted or added from counting result Live Dead Delete Cancel Add EVE Automatic Cell Counter 2013 NanoEnTek Inc 27 5 Click Apply changes button to make the changes 6 You can check cell size graph Cell size graph will be shown with different color for viable cells dead cells and total cells Using each button you can check individual result for viable cells dead cells and 7 If modified image should be saved then click Save changes total cells 28 Cell gating 1 Select the saved file you wish to open and adjust range of cell size using Cell gating button Automatic cell counter 2 Determine the value for minimum cell size and maximum cell size using up and down arrow button Minimum cell size is used to determine the low range of cell size to inclu
14. cell counter uses state of the art optics and image analysis to automatic cell counting The EVE is a benchtop counter designed to measure cell count and viability live dead and total cells accurately and precisely using the standard trypan blue technique Using the same amount of sample that you currently use with the hemocytometer the EVE takes less than 20 seconds per sample for a typical cell count and is compatible with a wide variety of eukaryotic cells and provides information on cell size The EVE offers an intuitive user interface and provides the option to save and print cell count data using the EVE Software download from www nanoentek com and USB drive supplied with the instrument or available separately The EVE is supplied with disposable EVE Cell counting slides that contain two enclosed chambers to hold the sample to allow you to measure two different samples or perform replicates of the same sample The cell counting occurs in the central location of the counting chamber and the volume counted is 0 4 uL the same as counting four 1 mm x 1 mm squares in a standard hemocytometer EVE Automatic Cell Counter 2013 NanoEnTek Inc 5 Features and benefits User friendly benchtop design for simple fast automated cell count and viability measurements within 20 seconds Provides data on cell size and is compatible with a wide variety of eukaryotic cells without the need for any special changes
15. de in the measurement The algorithm first identifies all objects and calculates the average size From the percent of average size setting the algorithm calculates the smallest object size to include in the final measurement Adjusting the number up increases inclusiveness thereby decreasing the lower cell size range Maximum cell size is used to determine the high range of cell size to include in the measurement The algorithm first identifies all objects then calculates the average size From the percent of average size setting the algorithm calculates the largest object size to include in the final measurement 3 After modifying cell size click Apply changes button to 4 You can check modified cell size graph will be shown EVE Automatic Cell Counter 2013 NanoEnTek Inc 29 PC software 1 If the cells clumping together are existed these will be counted for one cell by counting algorithm For this reason the cell counting result may not be accurate Counting In this case select the saved file you wish to open and click Counting button for re counting the cells exception algorithm will solve this problem User can achieve more accurate result 2 You can check more accurate counting result lt Result before recounting gt lt Result after recounting gt Before recounting cells clumping together could be counted one cell But after recounting software algorithm will recognize and count each of
16. e start the EVE by pressing the Power button 5 When the instrument is turned on the Start Up screen is displayed Here you can proceed immediately to cell counting set up the instrument for cell or bead count or adjust the screen brightness EVE Automatic Cell Counter 2013 NanoEnTek Inc co Operation Settings 1 Press Settings from the Start Up screen to display Settings The settings menu allows you to set up the following Count mode to operate the instrument for cell counting choose Cells or bead counting choose Beads Parameters see below and next page for details Calibration to calibrate the instrument page 23 Update to install new firmware versions as they become available Date and Time to set up date and time page 13 Use the scroll buttons to adjust the screen brightness Cell mode 1 Press Parameters from the settings screen to display Cell parameters mode parameters screen The Parameters function allows you to change the image analysis algorithm for specific or mixed cell types and the specific parameters must be determined empirically we y Protocol XFAIRT 2 The cell mode parameters are described below Sensitivity refers to the contrast of the objects from the background Adjusting the sensitivity higher makes instrument more sensitive to objects useful for cells that do not stain well with trypan blue while adjusting the sensitivity lower
17. ent is intended for use in an industrial environment In the documentation for the user a statement shall be included drawing attention to the fact that there may be potential difficulties in ensuring electromagnetic compatibility in other environments due to conducted as well as radiated disturbances 38 Review and follow the safety instructions below Symbol A c FCC compliance Meaning Caution amp Warning Protective earth Ground This instrument and consumables conforms to the Declaration of Conformity This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense WEEE Waste Electrical and Electronic Equipment symbol indicates that this product should not be disposed of in unsorted municipal waste Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of WEEE
18. g you can turn off the instrument by pressing and holding the Power button for 4 seconds EVE Automatic Cell Counter 2013 NanoEnTek Inc 19 Transferring data to a computer 1 To archive your data or generate a printed report insert EVE USB drive into the USB port O e O USB port A 2 Save your data on the USB drive by pressing the Save button on the main screen The image and the counting data are saved 3 Enter the file name using the keypad buttons displayed on the Save menu The numerical data is also automatically saved as a CSV file that can be opened with any spreadsheet program To delete all data from the CSV file and start with a blank file press Start CSV file button Start CSV fle 4 Transfer the EVE USB drive to the USB port on your PC You may open the CSV file using a spreadsheet program To see the image and generate a report use the EVE PC software on page 25 20 Using the 1 Press Settings and then press Beads to place the instrument beads mode into bead counting mode for counting beads 2 Put 10 uL of beads to 10 uL of 0 4 trypan blue stain and mix well Mix gently by pipetting up and down 3 Load 10 uL of the sample mixture on EVE Cell counting slide side A or B using pipette The two chambers of the slide are labeled A and B for easy tracking of your samples 4 Insert the EVE Cell counting slide with beads
19. her material enters the instrument the adaptor or power inlet disconnect the power cord andb contact a service person For operating environment refer to Product Specifications Do not touch the main plug or power cord with wet hands Always ensure that the power supply input voltage matches the voltage available in your location This instrument is air cooled so its surfaces may become hot during operation When installing the instrument leave a space of more than 10 cm 4 inches around it and do not place any objects between the instrument and the wall Do not install the instrument on a slant or a place prone to vibrations which induces the risk of instrument malfunction or damage of the instrument Never insert any objects into the air vents of the instrument as this could result in electrical shock personal injury and equipment damage Plug the power cord firmly into the wall outlet and AC adapter To avoid potential shock hazard make sure that the power cord is properly grounded Be sure to position the equipment such that it is easy to disconnect the instrument Turn off the instrument before unplugging the power cord and or moving the instrument If the instrument is broken or dropped disconnect the power cord and contact a service person Do not disassemble the instrument Use only authorized accessories adaptor power cord and USB drive Class A equipm
20. into the slide port on the instrument making sure that the sample side is inserted completely into the instrument 5 Press Count beads button Countbeads You can check the result in detail pressing More data button More data EVE Automatic Cell Counter 2013 NanoEnTek Inc 21 6 Adjust the bead image by pressing the Zoom button Navigate the fields by pressing the location you like to see on the grid 7 When you are satisfied with the image press the Count beads button Countbeads _ 8 The instrument takes approximately 20 seconds to count each sample and the bead count is displayed in the screen Record the bead count 9 To count beads in the other side of the slide chamber side B remove the slide after side A is counted turn the slide around and reinsert into the slide inlet to repeat the counting procedure 10 After counting beads place instrument into Cell Count mode for counting cells by pressing Settings button and then pressing the Cells button Calibrating 1 Press Settings and then press Calibration button Settings Callbration 2 Check the current background level and press Yes button to recalibrate This function sets a new background level Current background level is 6 Calibration Do you want to recalibrate 3 To recalibrate the EVE mix 10 uL trypan blue solution with 10 uL of a standard buffer such as phosphate buffered sali
21. makes the instrument less sensitive and is useful if there is a lot of background Minimum cell size is used to determine the low range of cell size to include in the measurement The algorithm first identifies all objects and calculates the average size e g 15 um From the percent of average size setting the algorithm calculates the smallest object size to include in the final measurement e g 70 of 15 is 10 5 um 15 10 5 4 5 um 4 5 um would be the smallest particle included in the count Adjusting the number up increases inclusiveness thereby decreasing the lower cell size range e g 50 of 15 is 75 um 15 Z 5 7 5 um Maximum cell size is used to determine the high range of cell size to include in the measurement The algorithm first identifies all objects then calculates the average size e g 15 um From the percent of average size setting the algorithm calculates the largest object size to include in the final measurement e g 200 200 of 15 um 30 um 30 um is the largest cell size included in the measurement Circularity is used to determine the objects to include in the measurement based on roundness Increasing the value from 80 requires objects to be more round for inclusion in the measurement Decreasing the value from 80 allows objects to be less round Adjusting this may be useful if the cell type is not particularly circular or perhaps oddly shaped After modifying any parameters press Apply
22. nd Tower 235 2 Guro3 dong Guro gu Seoul 152 740 Korea Tel 82 2 6220 7913 Fax 82 2 6220 7721 The information in this manual is described as accurately as possible Firmware and software changes and updates may change without prior consent or notification Copyright 2013 by NanoEnTek Inc All rights reserved Published in Korea Documentation NESMU EVE 001E Revision History V 0 0 Date MAR 2013 EVE Automatic Cell Counter 2013 NanoEnTek Inc 43 EVE_ Automatic cell counter NESMU EVE 001E V 0 0 NanoEnTek NanoEnTek Inc HQ 12F Ace High end Tower 235 2 Guro3 dong Guro gu Seoul 152 740 Korea Tel 82 2 6220 7913 Fax 82 2 6220 7721 NanoEnTek USA Inc 5627 Stoneridge Drive Suite 304 Pleasanton CA 94588 USA Tel 1 925 225 0108 1 888 988 0108 Toll free Fax 1 925 225 0109 Website www nanoentek com
23. ne PBS Mix thoroughly Calibrate the instrument this function sets a new background level 1 Mix trypan blue 1 1 with buffer 2 Load counting chamber with 10 pL 3 Insert counting slide 4 Press Start EVE Automatic Cell Counter 2013 NanoEnTek Inc 23 4 Load 10 uL of the sample mixture to the chamber ports on one side of the EVE Cell counting slide Press Start 5 After calibration is completed press the OK button to restart the instrument and proceed to cell counting There is no need to recalibrate each time the instrument is turned on 24 PC software Installation The EVE is designed for stand alone use and does not require the use of an external computer If you wish to archive data and generate reports you must transfer data to your computer and use the EVE software to generate and print reports see instructions below Alternatively data stored in the CSV file may be transferred to your computer by the USB drive and imported into any spreadsheet program without the need for EVE PC software Computer requirements EVE is not compatible with Macintosh operating systems USB port 1 1 or later 2 0 is recommended Windows XP 2000 Vista 7 1 Insert the supplied USB drive into the computer Then open the file EVE PC Software 2 The start up dialogue of the software will appear Click Next to start installation Wh instalation
24. tek warrants that EVE will be free from defects in material and workmanship for a period of one 1 year from date of purchase If any defects occur in EVE during this warranty period NanoEn Tek will repair or replace the defective parts at its discretion without charge The following defects however are specifically excluded Defects caused by improper operation Repair or modification done by anyone other than Nanoentek or an authorized agent Damage caused by substituting alternative parts Use of fittings or spare parts supplied by anyone other than NanoEnTek Damage caused by accident or misuse Damage caused by disaster Corrosion caused by improper solvent or sample NO N I W For your protection EVE being returned must be insured against possible damage or loss NanoEnTek cannot be responsible for damage incurred during shipment of a defective instrument It is recommend that you save the original packing material in which the instrument was shipped This warranty is limited to the replacement of defective products For any inquiry or request for repair service contact sales nanoentek com or your local distributor EVE Automatic Cell Counter 2013 NanoEnTek Inc 37 Safety precautions Review and follow the safety instructions below Do not install the instrument in a humid place such as a greenhouse or an incubator to avoid a danger of electric shock If water or ot
25. the date time is set there is no need to set it each time the instrument is turned on 5 Press Close button to exit the screen EVE Automatic Cell Counter 2013 NanoEnTek Inc 13 Recommend actions To obtain the best results follow these recommendations Wear Protective gloves during sample handling Do not touch the optical surfaces of the EVE Cell counting slides Hold the cell counting slides by the edges Use the EVE at room temperature only 5 40 C For accurate viability count results ensure the counting area is covered with cell suspension and count cells within 3 minutes of mixing the cells with trypan blue solution as trypan blue is toxic to cells For best data with biological samples we recommend counting at least two samples and taking an average The EVE is supplied pre calibrated To recalibrate your instrument see page 23 The EVE memory holds one set of data Save your data to the USB drive after each reading You may transfer the data to your PC using the USB drive immediately as described in Transferring data to a PC page 20 After using EVE appropriately dispose slides as biohazardous waste Do not reuse the cell counting slides 1 Push the Power button to start the instrument The Start up Cell counting screen is displayed 2 The EVE is preset to Cells mode 3 Put 10 uL of your sample to 10 uL of 0 4 trypan blue stain and

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