Coded Wire Tagging Operations Protocol for Chinook Salmon in the
Contents
1. 1 Position T4 Attach extender to fish outfall and position assembled we T4 See Appendix 1 for assembly under water flow and above 2 holding tank Figure 4 Adjust leg height so top edge of funnel 1s parallel to ground and at correct height for fish outfall Water from fish outfall s sufficient to move fish through T4 however a hose attachment 1s located under the funnel 1f additional flow 1s required Add a fish live car under the right side of the diverting gate to catch unmarked fish Marked fish should fall into holding tank Adjust flow so fish are sliding easily through tunnel and wa ter coming out back of detector 1s hitting surface water of holding tank about 30 cm from end of tunnel 2 Attach power supply and cords The two 8 pin connectors are Figure 4 T4 positioned between fish outfall of identical on the T4 Detector and control box Figure 5 The uni trailer and holding tank versal cables are also identical at each end To insure good elec trical contact and waterproof assembly 1 gently push and rotate plug in connector until plug hits connector pins 2 push plug as far into connector as 1t easily goes and 3 turn locking ring a half turn while continuing to push until ring and connector are firmly attached Note Make sure cables and connectors are dry before attaching and close dust covers when not in use e Connect cord from control box to T4 e Connect cord from control box to diverting gate2. Overnight Escaped Tagged Untagged Release Date le Appendix 3 Coded Wire Tagging Data Sheet continued Sequential Tag Information Date Page____ Fish Length FL mm Fish Weight g Initial Tag Final Tag Tag Code Notes Comments AE F dl Bda CRAMER FISH SCIENCES Copyright 2007 by Cramer Fish Sciences
3. T pump u not enter the trailer and generator 1s clear from all potential fire hazards like dry grass or brush Connect red extension cord between trailer service panel and generator 2 Connect water pump Remove water pump and hose connections from trailer Check water tightness of water pump cord Station water pump Figure 1 and attach hoses to trailer Figure 2 Plug water pump into generator D Start generator As generator starts check water flow and supply inside Figure 2 Hose attachments to C WT trailer Blue hose delivers water from pump green hose delivers internal freshwater overflow back to river and outside trailer Check water pressure inside trailer and make sure sinks do not overfill Adjust water pressure at sink valves 1f necessary Water flow will fill inside and outside holding tanks Make sure tanks do not overfill Prepare Tricaine S dope water Fill red tank to within 2 inches of top 15 gallons Figure 3 Add 1 tsp of Tricaine S powder to red tank and stir Check pH with paper and add 1 1 tsp baking soda as needed to achieve neutral pH 7 Prime small water pump with water from tank and plug into outlet above sink Pump will start re circulating dope water Open sink valves and fill sinks check pressure at sink valves and do not overfill Figure 3 Red Tricaine S water tank a l NB CODE WIRE TAGGING PROTOCOL AT CASWELL STATE PARK B T4 Detector Setup
4. solution on electrical outlets caps connections of power supply and touch switch and allow to dry before replacing caps Dry soaking injector parts and wipe down tools and other equipment in tap water Remove any debris CWT tags attached to cutter parts Use tag wire dipped in alcohol solution and probe holes in cutter to clean Force a stream of alcohol through tagging needle with an alcohol loaded syringe Reassemble parts using disinfected tools T4 Detector Unplug power and beeper cables from back of detector and put caps over connectors before washing with dilute bleach solution Be sure connectors are dry before closing caps Remove power supply and wipe dry Wash tunnel gate and fish funnel thoroughly to remove scales and fish slime To disinfect the T4 wipe clean with dilute bleach solution CODE WIRE TAGGING PROTOCOL AT CASWELL STATE PARK 6 405 Appendix 1 T4 Assembly gt _ P 2 Clip T4 on to pins at lower end 3 Fit high end into grooves and secure with clips l j i J d 4 Attach diverting gate fit pins 5 Attach funnel fit pins to 6 Assembled T4 to holes clip to T4 holes and clip to T4 IP gt 19 PX GG p GG 7 e 7 Attach control box with wing nuts 8 Universal cords are used to connect 9 Turn locking ring to attach cords and screws above funnel components place duct tape over bell to reduce sound volume o
5. Coded Wire Tagging Operations Protocol for Chinook Salmon in the Stanislaus River A Detailed Protocol for Coded Wire Tagging Operations on the Stanislaus River at Caswell State Park Prepared for U S FISH 8 WILDLIFE SERVICE amp U S BUREAU OF RECLAMATION Prepared by Ayesha Gray Ryan Cuthbert amp Brad Cavallo CRAMER FISH SCIENCES Copyright 2007 by Cramer Fish Sciences CRAMER FISH SCIENCES 636 HEDBURG WAY SUITE 22 OAKDALE CA 95361 209 847 7786 WWW FISHSCIENCES NET CODE WIRE TAGGING PROTOCOL AT CASWELL STATE PARK Table of Contents ll TOGUE EVO raid pi no 1 Il NEeIErenNcES s z too o uk tina non ohna plni aliada 1 W SEUD ee SEE 1 A Water and Power Setup ee 1 b A DECECO OUD arcas 2 E Mark IV Injector SEE aaa cate 2 V PM AAA E a 4 Ao AP A 4 A Half Length Coded Wire Tags 35 60 MM nn 4 B Sequential Decimal Coded Wire Tags gt 60 MM 5 VU BIESKAOWN euren i nov ale oe oo 6 Vl MAIM ENING nen in SK edk S po ir 7 A Disintecting ABC rosana esos O aAa 7 B MAK IV MMO CL OM een lese nie eine release sues T U TA DOCO 0 eee osn ee ee ee eee T Appendix 1 T4 Assembly ooccoccocccnccocnonccnncnnconccnnonanonrnnnnnnnnnrnnnnnncnnrnnrnnnrnnrnnrnanennenarnanen 9 Appendix 2 Disassembling and Cleaning the MKlV o occconconocinccconcnoncconanonanonannns 10 Appendix 3 Coded Wire Tagging Data SheetS occooncccncoccccccccocacoccconanonnnonanonannos 11 CODE WIRE TAGGING PROTOCOL AT CAS
6. WELL STATE PARK I Introduction The following protocol describes the procedure for coded wire tagging operations on the Stanislaus River at Caswell State Park including set up and equipment operation and maintenance determining tag depth and placement tag ging fish handling release and mortality tag retention studies Refer to the Detailed Study Plan for Coded Wire Tagging for additional information about the project Il References Refer to the T4 Detector User s Manual Mark IV Instruction Manual and Coded Wire Tag Project Manual for additional information from Northwest Marine Technology NMT regarding use and setup of equipment These manuals can be found on the NMT website http www nmt us support manual htm III Setup When the trailer 1s used at a remote site 1 e away from a hatchery or other facility providing electricity and security it will be necessary to set up power generator water pump from the river marking eguipment fish holding tanks etc on a daily basis At the end of the day eguipment will need to be put away and secured Note Generator water pump hose connections and T4 Detector will all be stored within the tagging trailer when not in use A Water and Power Setup 1 Position generator Generator should be positioned several meters away from the T4 Detector as a magnetic field created by generator may cause false positives by T4 Detector Take care to insure generator exhaust does sea
7. d dead through T4 Detector and determine number of tagged and untagged fish Record number of tagged and untagged fish on data sheet Label and bag all mortalities and return to the office freezer If fish are released at night a night crew will also check for mortalities and record any additional mortalities on data sheet Mortalities and tag retention numbers will be reported to Jason Guignard CDFG each week Tagged fish may be held in river net pens until nighttime release or released immediately after QC Tagged fish will be released below trapping site into the river in small groups to avoid predation Releases of marked fish to determine trap efficiency may also use tagged fish Sequential Decimal Coded Wire Tags gt 60 mm One marking station will be dedicated to tagging larger fish with sequential tags A larger head mold will be used for these fish Larger fish will be segregated from day s catch and held sepa rately from fish marked with half length tags mum efficiency of process Each tagger will follow instructions detailed above regarding water conditions marking small groups of fish at a time Tricaine S exposure etc from the MKIV read with the Magni viewer Figure 12 taped to the datasheet and tag number recorded on data sheet see Appen dix 3 If fish are to be individually marked flanking tags initial and final will be ejected and taped to the datasheet These tags will be read with the Magni view
8. e Connect cord from T4 to power supply ll f ZD o Figure 5 T4 control box displaving the identi supply can be used to power the T4 Slide the on off switch on E conector io Cane con battery pack to the on position The green power ON Led will trolled using duct tape red arrow which can light Charge the battery pack for 8 hours before use for maximum De removed so alarm is heard inside the trailer capacity When using the 24 V DC power supply plug into the 3 prong outlet on generator Note The cord is glued into the power supply so please do not try to remove it 4 Check T4 function using detection standard The detection standard a CWT on the end of a stick can be used to verify T4 1s detecting tags determine range of speeds a tag 1s detected and to make sure live car 1s positioned under the correct side of the diverting gate for catching unmarked fish Note When T4 detects a tag the diverting gate will open moving tagged fish into holding tank or live car C Mark IV Injector Setup 1 Setup MKIV Place MKIV at each tagging station above round sink Attach power supply to small connec tor at rear of MKIV run cord through ceiling hooks and plug into AC outlet on wall Note The MKIV has an internal breaker and protective fuse which will turn off the MKIV when tripped After 1 minute the breaker will reset unless the protective fuse has blown in which case NMT servicing is required Attach touch switch to either of the large con
9. er after completion of day s tag ging to avoid slowing the tagging process and tag numbers will be recorded on datasheet If fish are to marked in daily batches after initial tag is ejected and read fish are tagged then final tag is eyected read and saved Initial and final tag numbers are recorded on datasheet Figure 12 NMT Magni viewer top used to read seguential tags bottom CODE WIRE TAGGING PROTOCOL AT ASWELL STATE PARK 4 After fish 1s tagged place in round sink below tagging machine Make sure water pressure 1s sufficient to move fish efficiently through pipe Fish will travel down pipe and exit trailer into T4 Detector funnel Fish will be counted if tagged as 1t moves through T4 Detector and will be deposited into external holding tank Untagged fish will be diverted by T4 Detector into live car in external holding tank Fish from live car should be retrieved often re anesthetized and re tagged Note Do not attempt to tag any fish more than twice If tagging fails twice fish will be released untagged Tagged fish will recover in external holding tank for at least one hour Make sure tank is fully shaded and water temperatures are low 1 e lt 16 C 60 F After all the day s catch are tagged and have recovered in exter nal holding tank fish will be carefully counted manually and the number of fish tagged will be compared to number as counted by T4 and batch count on each MKIV Press BATCH on k
10. eypad to display number of fish tagged at each station Record each number T4 MKIV and manual count on data sheet Press CLEAR and MKIV will display OK TO CLEAR COUNTS so press OK to clear Fish tagged with sequential tags will be held for tag retention and mortality estimates separate from fish tagged with half length tags so tag retention and mortality estimates are specific to tag type Follow instructions above for holding and releasing fish Vi Breakdown 1 Flush all sinks with 5 gallons of river water to make sure all tagged fish are in the external holding tank After flushing tanks empty dope water on to ground at least 20 meters away from river using the small water pump and a hose Turn on water and pump all dope water out of red tank Vote Do not dump large amounts of water on dike road Please make sure it is drained well off the side Fill a couple of buckets with water to be used in cleaning trailer floor then shut off generator Use mop and squeegee to clean floor of trailer Sinks and tabletops will be wiped clean with dilute bleach solution MKIV tagging button and power supply will be disconnected and stored and MKIV will be cleaned after each use following instructions in Appendix 2 Make sure all supplies and equipment are stored properly Let generator cool outside before putting in trailer Once a week floors will be thoroughly mopped with disinfectant dilute bleach solution T4 Detecto
11. h will be released untagged Cope WIRE TAGGING PROTOCOL AT CASWELL STATE PARK 8 Tagged fish will recover in external holding tank for at least one 10 B 1 Fish greater than 60 mm will be marked with seguential tags Processing and marking area will be setup to achieve the maxi Before marking begins the initial sequential tag will be ejected hour Make sure tank is fully shaded and water temperatures are low 1 e less than 16 C 60 F After all the day s catch are tagged and have recovered in external holding tank fish will be carefully counted manually and the number of fish tagged will be compared to number as counted by T4 and batch count on each MKIV Press BATCH on keypad to display number of fish tagged at each station Record each number T4 MKIV and manual count on data sheet MKIV will display OK TO CLEAR COUNTS so press OK to clear Figure 11 Tagging juvenile Chinook Esti F l j 11 l th stimates of tag retention and mortality will be determined wit with MKIV a sub sample of tagged fish Retrieve 3 of tagged fish after each tagging session and put them in a net pen in a protected location in the river Label marking tape on net pen with date number of fish batch code number location and purpose tag retention and mortality or effi ciency release After 24 hours check the net pen remove all fish and count Record the number of mortalities on data sheet Run all fish live an
12. nectors at rear of MKIV Keep the protective caps on connectors when not in use Make sure appropriate head mold is on machine for size of fish and it is in proper position with sides parallel to ground and head mold cup up Figure 6 CODE WIRE TAGGING PROTOCOL AT CASWELL STATE PARK 2 Turn on MKIV Power MKIV by pressing rubber covered on off button on back of MKIV The MKIV will show the message NO QCD OK Press OK on keyboard to confirm and clear message as we are using a T4 Detector instead of a in line QCD The READY V7 0 message 3 Inspect tagging needle Press SHOW on keypad and examine needle with magnifier Make sure 1t 1s sharp and smooth and the needle position 1s correct with bevel angled up After inspection retract needle by hitting ESC on keypad 4 Engage rollers Open latch on front of MKIV and swing open M i door Find lower drive roller arm and move lever to engage Figure 6 Headmold positioned on Mark IV rollers Figure 7 Note Rollers should always be left disengaged tag injector after use 5 Load tag wire Put the MKIV in Load mode by pressing LOAD on keypad This will disengage motor drive Remove the label on one side of the tag wire and attach to datasheet for sampling week Add spool and thread tag wire through rear wire guide until it reaches drive rollers Rotate roller clockwise by hand to feed wire into front wire guide Use hemostats from tool kit to start wire into forwa
13. owever the margin for error is larger 8 MKIV is ready to mark fish Figure 8 CWT in Chi nook salmon fry CODE WIRE TAGGING PROTOCOL AT CASWELL STATE PARK IV Final Check 1 2 Check temperature and Dissolved Oxygen DO Make sure temperature of both fresh water tanks and dope water ranges from 10 19 C 50 65 F and check dissolved oxygen with DO meter Dissolved oxygen should be at 95 saturation Dope water temperature can be lowered if necessary by adding frozen water bottles If DO is low add an aerator to holding tanks to increase oxygen levels Check flow Make sure all sinks have adequate flow to move fish through pipe system without too much splash and spray on MKIVs V Daily Operations A Half Length Coded Wire Tags 35 60 mm 1 Each tagger will record and monitor water conditions at their The tagger will take small groups of fish 10 individuals Carefully pick up fish cradling head between thumb and index Position fish upside down in head mold be sure proper size After fish is tagged place in round sink below tagging machine Each day s fish are transferred to trailer s internal holding tank Figure 9 from screw trap live box using buckets station including water temperatures dissolved oxygen satura tion and time to sedation Each water sink has a digital ther mometer to constantly monitor water temperature from internal holding tank with dip nets Fish will be pu
14. ptional a GSAS ned under fish Appendix 2 Disassembling and Cleaning the MKIV A 1 Disengage black latch AM cutter daily M 4 Unscrew top nut on cutter as sembly with 7 64 hex wrench 7 Examine hole with magnifier for damage or wear 10 Pull out needle and examine tip for damage or wear with magnifier 13 Remove needle carrier and use tag wire to check for stray tags 10 5 Hold and unscrew lower nut on cutter assembly 6 Remove head mold with 1 1 6 hex wrench 11 Unscrew needle carrier nut with 3 32 hex wrench 14 Clean head mold with bleach solution ONLY 6 Pull out cutter bar use tag wire to check for stray tags 9 Carefully remove tagging needle 12 Use hemostats to remove needle carrier 15 Clean interior of MKIV with alcohol and soak all internal parts in dilute bleach solution Appendix 3 Coded Wire Tagging Data Sheet Tagging Crew Members Pretagging Released Morts Untagged Half Sequential Comments Half Tag Information Batch Code Tagging Morts Release Held after Tagging Overnight Field Check Entered By FP 3CRAMER F 41 FISH SCIENCES mi Half Tag QA QC QC Crew Overnight Escaped Tagged Untagged Release Efficiency Members Morts Date Mark QC Crew Members
15. r will be shut off cords detached and dried T4 tunnel and funnel will be cleaned using dilute bleach solution and paper towels Make sure caps on connectors are dry and closed Store T4 inside trailer Neatly stack all hoses inside trailer Generator water pump T4 Detector will be placed carefully in trailer CODE WIRE TAGGING PROTOCOL AT CASWELL STATE PARK VII Maintenance A Disinfecting Eguipment Equipment should be cleaned and disinfected at the end of each tagging session See Appendix 2 for additional photos and instructions for cleaning the MKIV B 1 C Wipe marking counter with dilute bleach solution Make sure all Remove head mold and wipe clean with bleach solution DO Open MKIV and remove tagging needle needle carrier clamping Mark IV Injector Wipe down exterior surfaces of MKIV with a damp paper towel including the touch switch cords and power supply outlets are capped on MKIV and wipe all exterior surfaces with bleach solution NOT USE ALCOHOL ON HEAD MOLDS nut and dismantle the cutter Figure 13 see also Appendix 2 Place in container and cover with bleach solution Remove head mold positioning screws and place with tools in same solution Figure 13 Technician removing the cutter bar With the alcohol spray bottle wet and clean the interior surfaces Carefully clean all surfaces and tubes and shift parts to make sure cleansing agent reaches all surfaces Spray alcohol
16. rd guide Re engage rollers and turn top roller clockwise until tag wire extends slightly beyond top of the head mold Press the ESC key The MKIV will be in Tag mode Press TAG on keypad once to cut extra tag wire MKIV is now ready to operate Figure 7 Internal parts of the Mark IV injec tor including the green drive rollers and drive roller arm red arrow 6 Check tag placement To set penetration put MKIV in SHOW mode which will move needle to its fully extended position Loosen set screws on head mold and slide head mold in or out to adjust distance needle will extend into specimen Gently tighten set screws to hold head mold in place Note Correct placement for the com monly used head molds will be marked by pencil or waterproof marker Tag placement can be confirmed in very small fish 35 40 mm by opening the mouth and looking ventrally through the nose 7 Tag fish and check placement Tag should appear centered in the sinus cavity of the small fish Figure 8 If tag is too deep move the head mold out slightly If tag is too shallow move the head mold in slightly These movements need to be very fine small differences e g 0 5 mm can be extreme in small fry Repeat procedure until ideal tag placement is achieved Mark position on head mold with pencil or waterproof pen so approximate position of head mold 1s known the next time tag placement 1s set Note Setting tag placement in larger fish is done the same way h
17. t into net baskets and placed in dope water sink at a tagging station when tagger is ready to begin marking Fish will be anesthetized after 2 5 minutes When fish reach Figure 9 CWT trailer s internal holding tank sedation and exhibit loss of equilibrium marking will begin If fish are in dope water longer than 5 minutes they can be transferred to adjacent fresh water sink to recover finger Position fish so adipose fin is resting on middle finger Figure 10 Use surgical scissors to cut off adipose fin being careful not to cut fish or other fins Note Be sure to remove at least 50 of the adipose fin to prevent later regrowth head mold 1s being used When fish 1s secure and firmly but gently pressed against MKIV push tagging button Make sure p the fish s head stays firmly against the tagging machine while Y by SN tag is placed and does not move away from machine Figure 11 Figure 10 Cutting adipose fin with surgical SCISSOVS Make sure water pressure 1s sufficient to move fish efficiently through pipes Fish will travel down pipe and exit trailer into T4 Detector Fish will be counted if tagged as it moves through T4 Detector and will be deposited into external holding tank Untagged fish will be diverted by T4 Detector into live car in external holding tank Fish from live car should be retrieved re anesthetized and re tagged Note Do not attempt to tag any fish more than twice If tagging fails twice fis
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