Plate reader protocol
Contents
1. Boston University Biomedical Engineering Department Biointerface Technologies Core 44 Cummington Street Boston MA02218 Molecular Devices SpectraMax M5 Plate Reader Protocol 1 Turn on the plate reader The power switch is at the back on the right hand side The plate reader will go through a self testing routine It takes a couple of minutes and will make a lot of clicking noise 2 Turn on the computer if it is not already on Open the SoftMax Pro program by double clicking the icon on the desk top 3 Check the plate reader icon at the top left corner of the SoftMax Pro window e If there is a question mark on it it means the plate reader is not ready e If there is a red cross on it it means the computer and the plate reader are not communicating Click on the icon and make sure the Serial Port is set at COM4 and the Reader is set at SpectraMax MS 4 There are three ways to set up your experiment e The SoftMax Pro has many commonly used assays stored Go to Protocols and select the type of assay you are performing Double check the settings and select the sample wells e If you prefer to set up your own experiment go to Experiment gt New Plate or New CuvetteSet click the Settings button under the new plate or new cuvette window and set up your parameters e If you want to use a saved experiment setting go to File gt Open and select your file to open 5 Put your plate in the tray and click Read button beside the plate rea2. der icon Remember to use the purple plate adaptor if you are using a 96 well plate or a 384 well plate top read If you are using a cuvette put in your reference blank click the Reference button then put in your sample and click Read 6 If you want to save your experimental settings go to File gt Save If you only want to save your data go to File gt Import Export gt Export and save your data as Text Files You can then import the text file into Excel 7 Save all your files in folder c plate reader your group name your name You can then transfer your file either through email or using a flash drive 8 When you are done close the drawer and the SoftMax Pro program If it is early in the day leave the plate reader on for other users If it is late in the afternoon 5pm turn off the plate reader 9 Wipe off any spill with wet paper towel Empty any liquid in the sink before dispose the plate in regular trash If your samples are of BL1 or BL2 disinfect with bleach before empty down the sink 10 Sign the use log For more detailed instructions on setting up the plate reader see the User Manual Boston University Biomedical Engineering Department Biointerface Technologies Core 44 Cummington Street Boston MA02218 For technical support contact David Micha at 408 747 3678 or email David_micha moldev com
Download Pdf Manuals
Related Search