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1. Step Action 1 Choose Administration System Setup in the UNICORN Manager Result The System Setup dialog box is displayed e p 123 6 Administration 6 2 System administration 6 2 1 System definitions 03 0014 93 ep 124 Step Action e Select click the icon of the system you want a summary of e Click the Summary button Result System Table Summary dialog box is displayed System Table Summary F x System 1 The system is a chromatography AKTA system Control unit 1 at computer HOME controls the system Backup is taken every 5 minutes Strategy in use E100F400 The strategy is designed for the systems AKTAExplorerl 00 Air Strategy Version 4 00 Required Unicorn version UNICORN 4 12 or later Required instrument versions 4D 900 v1 00 or later Air Sensor Air 900 v1 01 or later AutoS ampler 4 900 v1 04 or later AutoS ampler 4 905 v1 00 or later Fraction Collector Frac 950 V1 00 or later pH C monitor pH C 900 v1 01 or later Sample Pump P 950 v1 12 or later Sample Pump P 960 v1 00 or later System Pump P 900 v1 40 or later UY Monitor U 900 v1 04 or later Valves v1 04 or later Compiled with xl Phamka mas Milde Ot e Click the Print button to print out the information e Click the Close button to exit the dialog box 6 2 2 Purpose The different types of audit trail files Tabs of the Audit trail dialog box How to view the audit trail
2. eC Create a new UNICORN user The created user has default set as logon password User name p avid Eull name p avid Wiggans Position Power user Home folder Drive c Name DW iad OK Cancel Help ep 147 6 Administration 6 3 User Administration 6 3 3 How to create a new user Home folders How to create subfolders to the home folder 03 0014 93 ep 148 General Each user should be assigned to a home folder preferably created on a network drive If the home folders are created on the local drive C they will not be accessible from other computers Network installations Always create home folders on a network drive which is accessible from all computers Make sure that the drive is addressed by the same drive letter from all computers in the network Non network installations A home folder can always be created on a network drive even if UNICORN is not installed for network control The computer only needs to be connected and logged on to the local network The table below describes how to create new folders in the home folder Step Action In the UNICORN Manager choose File New Folder Result The Create New Folder dialog box is displayed e Type the new folder name in the dialog box e Click OK Note This operation can be performed by each user in their own home folders 6 3 4 Introduction How to open the User properties dialog box
3. e In the Action field make sure that the Create option is selected e Click the Browse button to select where to store the backup file Note Select A to store the file on a diskette e In the Items field select which information to include on the backup file e Click the Create button to create the backup file and store it in the selected location The created backup file has the ending bck Note You can click the Information button to see which inform ation files will be included in the backup file How to restore The table below describes how to restore the system definitions from a backup file in defini ocated for example on a rescue diskette Note Any user can restore their personal files To restore global and system files the user needs the access item Edit global list s see 6 3 2 Access items on page 142 ep 135 6 Administration 6 2 System administration 6 2 3 How to back up and restore system definitions 03 0014 93 ep 136 If the backup file is located on a diskette insert the diskette into the computer Choose Administration Create Restore Backup in the UNICORN Manager to display the Create Restore Backup dialog box Create Restore Backup x Action C Create Restore Browse C UNICORN Items Global Files Personal Files C System Files Information Cancel Help e Ifthe backup file is located in a different place
4. Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box is displayed Choose View Renew interval Result The Renew interval dialog box is displayed x m Next renew The log file will be renewed at Renew now m Interval Renew log file every 7 days Set Close 03 0014 93 ep 130 Administration 6 Step Action Set renewal interval e Type the desired interval in the Interval field The standard value is 7 days e Click the Set button Result The new Interval setting will take effect after the next scheduled renewal or immediately if the Renew now button is clicked Renew now e Click the Renew now button to start a new audit trail file The old file is saved automatically Example If the audit trail is set to renew every 7 days and you click Renew now on a Friday a new file will be started immediately and another file will be started the following Friday Click the Close button How to back up The table below describes how to back up audit trail files audit trail files i oo i Note Old audit trail file are automatically saved each time a new one is created This backup function is only necessary if you want to copy or move the audit trail files to a specific location Step Action Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box is displayed Choose File Backup Result
5. IV Use large toolbar icons I Show unused variables Show variable details Il Default overwrite of baselines and peak tables Quick view curve f Fraction mark height 2 Character heights bs Injection mark height 50 Percent of window height x Logbook mark height 3 Character heights fe Cancel Help e Make the desired changes e Click OK 03 0014 93 p160 6 3 6 Introduction How to delete a user How to delete a folder Administration 6 How to delete users and folders This section describes e how to delete users and folders e how to delete special folders home folders with assigned users folders with shared access The table below describes how to delete a user Action Result The User Setup dialog box is displayed e Click OK to confirm the right to perform administration functions Click the Close button Choose Administration User Setup in the UNICORN Manager e Select the user from the Users list and click the Delete button Note You can delete all users except the last user with User setup Levels access This ensures that at least one user has Note When you delete a user the user s home folder or method and result files are not deleted Caution A deleted file folder cannot be recovered The table below describes how to delete a folder Result The User Setup dialog box is displayed Result The User properties
6. 2 3 1 How to install UNICORN Step 2 License agreement and user information 03 0014 93 ep 38 Step Action e Insert the CD ROM disk into the CD ROM drive The UNICORN Setup Program should start automatically If not e click the Windows Start button and select Run e type the command d setup where d is the unit for your CD ROM drive e click OK The UNICORN Setup Program is launched Continue the setup be low This table describes how to complete step 2 of the UNICORN Setup Program Action e The Welcome dialog box is displayed e Click the Next button to continue e The UNICORN Software License Agreement dialog box is displayed You must accept the license agreement to install UNICORN e Click the Yes button to continue e The User Information dialog box is displayed Type your name company and the product serial number of the software The serial number can be found on the UNICORN License Agreement that is shipped with the CD e Click the Next button to continue Installation 2 Step 3 Select Sys In the Select System Type dialog box you choose the type of system instrument you will use tem Type Step 4 Select Components Select System Type l x AKTA System except those listed below Ettan LC System AKTAxpress System AKTAoligopilot System BioProcess System lt Back Next gt Cancel Note In UNICORN 5 0 a dialog box with
7. If you connect from a local station to a chromatography system physically connected to the local station you do not have to log on to the network Be aware though that there are some drawbacks if you do not log on e Files stored on network drives will not be accessible e UNICORN is placed in an error state which is not ideal Global files such as the user settings file musers30 mpm etc are stored on the network Any changes made to these files while you are not logged on will apply only locally and will be lost the next time you log on to the network to use UNICORN e For runs performed in this stand alone mode the result file cannot be saved on a network drive If the file is directed to a network drive it will instead be saved in the Failed folder on the local station System connections 3 3 3 Connection modes Types of connec The user can establish two different types of connections to a chromatography tions system e Control mode connection The user is able to actively control the system e View mode connection The user can monitor the system activity but cannot control the system Several simultaneous connections can be established to one system but only one may be in control mode The other connections are in view mode Status bar inform The System Control module displays information in the status bar regarding the connection mode The table below explains the status bar text and the possible actions t
8. e Select the Security tab and click the Remove button to remove non authorized users and groups e Click the Add button and make sure the correct domain is dis played in the top drop down menu Select the newly created user group in the list box and click the Add button e Click OK Set the permission Full Control to the newly created user group S e N Click OK to accept sharing a folder e p11 1 Network setup 1 5 How to configure the workstations 1 5 How to configure the workstations Introduction This section describes how to configure the workstations in the UNICORN network depending on the operating system installed on the workstations In this section This section contains these topics Topic See How to configure Windows 2000 workstations 1 5 1 How to configure Windows XP workstations 1 5 2 03 0014 93 e p12 1 5 1 Introduction User rights How to set the correct user right Network compon ents to install Network setup 1 How to configure Windows 2000 workstations This subsection describes how to set up Windows 2000 workstations in a UNICORN network with a Windows server and TCP IP network protocol All users must have the Windows user right Access this computer from the network which is also the default user right If the user right is not correct the network administrator can change it Follow the instructions in the table below to set
9. Access Access to folders and systems Instructions Available manual instructions sounds 03 0014 93 ep150 The User item Administration 6 The illustration below displays the User properties dialog box with the User item selected User properties S Dave David Wilton i P Pdtibutes g Advanced E ow Access RA Instructions User Username Dae mae awn EE Postion PodRes Group Administrator X Home folder fc Default z Administrator Attributes I User disabled R Mutt change petsword amp next logon cot He xj The table below describes how to edit the User item of the User properties dialog box Action e Click OK e Select another item to edit if desired See further information be low Open the User properties dialog box see the table in beginning of this section and select the User item e Type a new password in the Password text box e Type the password again in the Confirm text box Note The password entries must be identical to be accepted Passwords are case sensitive in UNICORN e Select an access group from the Group drop down list Note A pre defined access group is assigned a certain level of access to UNICORN e Select a folder from the Home folder drop down list e p 151 6 Administration 6 3 User Administration 6 3 4 How to assign user properties The Attributes item 03 0014 93 ep152 The i
10. Predefined The following applies to the predefined groups e Each group has a name that reflects the status of its members such as Administrator and Process operator e Each group has different rights to perform actions in the UNICORN system e The names and rights of the groups can be changed to the requirements of each individual group Undefined If the system administrator wants to create access groups in addition to the predefined groups he or she can use the undefined access groups The following applies to the undefined groups e They have generic numbered names like Group 7 Group 8 and so on e They do not have any rights defined e Itis up to the system administrator to define names and rights to the undefined groups The access items The access items are used to assign rights to the access groups How to assign rights to the access groups is described in How to edit the access groups below Each access item is described in 6 3 2 Access items on page 142 03 0014 93 ep 138 The User Setup dialog box How to view the access groups Administration 6 From the User Setup dialog box you can e see which access group each user belongs to e view the access level of each access group e change user access groups Note Other actions which can be performed from the User Setup dialog box are described in subsequent sections of this chapter How to open the dialog box Choose Administra
11. Setup Type C Network installation Remote only system Note The Setup Type options are limited in the Remote and Dry versions of UNICORN as described in 2 3 1 Different UNICORN versions on page 36 The table below describes how to select the setup type Action You can perform either a Stand alone installation or a Network install ation see e 2 3 1 Step 6 Stand alone Installation on page 43 e 2 3 1 Step 6 Network Installation on page 44 When you have made your selections click the Next button to con tinue A stand alone installation can be either e a local station e a demo station Select the Demo system check box if you want to install a demo station The network options settings are ignored for a stand alone installation Note If you perform a stand alone installation and later want to connect the system to a network you must remove the current installation and install the software with the appropriate settings ep 43 2 Installation 2 3 Software Installation 2 3 1 How to install UNICORN Step 6 Network A network installation can be either installation Step 7 Program Options 03 0014 93 ep44 e a local station e aremote only system You have to select these disk drives in a network installation e A local disk for the program files e A server disk for the server files Select the Remote only system check box to install a remote only system that being
12. Strategy files 69 Subfolders to the home folder create 148 Index e pix Index System unlocked 79 locked 79 unlocked 81 locked 81 System Connect 74 System connections 74 System Control connect 75 System control connection security 91 System Control module 77 System control module disconnect system 81 System Control window 74 System Disconnect 74 System installation 71 System parameters default values 164 System recommendations 189 hardware 189 software 190 software 190 network 190 System run hours display 132 System security default user delete 90 maintain 90 T Template files Installation 47 Installation 63 Template files 69 03 0014 93 epx U UNICORN log off 82 quit 82 Unicorn administrator responsibilities 4 Unicorn network example configuration 8 Unicorn software installation in network setup using TCP IP and Windows 2000 16 in network setup using TCP IP and Windows 2000 20 Unicorn workstations local and remote stations 4 Unlock locked systems 79 Unlock locked systems 81 Unlocked system 79 Unlocked system 81 User default description 145 assign to a home folder 148 delete instruction 161 User access password rules 90 User access group the User Setup dialog box 139 User access groups 138 different kinds 138 access items 138 view 139 edit 140 User definition edit 151 User information 68 User passwords for a selected u
13. The Backup log files dialog box is displayed x Log fies Select a folder for the files Data 8 My Computer 3 SQ My Network Places ep 131 6 Administration 6 2 System administration 6 2 2 Audit trails How to display the system run hours 03 0014 93 ep 132 Step Action 3 You can choose either to copy or move the log files e Select the files you want to back up in the Log files field e Select a destination folder in the structure tree e Select to Copy or Move the file s in the Mode field The Move al ternative is recommended to save disk space e Click the Copy or Move button depending on the previous choice e Click the Close button Note Backup operations are recorded in the audit trail Run hour values show the number of hours that the system has been used for manual or method controlled runs The Run hours record is useful if you want follow up the expected and actual lifetimes for liquid handling components The table below describes how to display and reset the system run hours Step Action Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box is displayed e Choose a System tab e Choose View Run hours Result The Run Hours dialog box with the accumulated run time for the system is displayed 10 0 Hours Reset Help e If you want to reset the accumulated run hours to zero 0 click the Reset butto
14. a computer to which no systems are physically connected Note When you perform a network installation the necessary UNICORN software components will be copied automatically to the network server disk Follow the instructions in the table below to set the required parameters for password and system control windows Program Options x Set the requirement for minimum number of password characters and the number of system control windows Press the NEXT button to continue Password Password required Minimum number of password characters 3 No password required System Control Windows 4 C3 C4 lt Back Cancel Step Action 1 Select Password required and type the minimum number of characters required for passwords Valid numbers of password characters are 3 15 Select No password required if you do not require password protec tion With this setting users can be defined with or without pass words Note Make sure that you enter the same password settings on each station in a network Installation 2 Step Action Choose the number of System Control windows that should be avail able in the installation Maximum value is 4 3 Click the Next button to continue Step 8 Select In the Select Program Folder dialog box you choose where to store the program Program Folder jon Select Program Folder Ea Setup will add program ico
15. amp AKTAc Component Selection Program Files Global Procedures Global Report Formats The setup program is ready to copy the files The Start Copying Files dialog box displays all the selections that have been made and the components to be installed Note If you want to make any changes you can click the Back button one or more times If the settings are correct click the Next button to copy the files 03 0014 93 ep 46 Installation 2 Step 10 Strategy The Strategy and Template Installation dialog box is displayed if you chose to install and Template In stallation strategy and or template files in step 4 If you did not choose to install strategy and template files go to Step 12 System Table settings below Strategy and Template Installation x Select the strategy you want to install and press the INSTALL button If your strategy template is located on a floppy or on another source press the HAVE DISK button to locate it When all strategies templates are installed press NEXT to continue Options Type 2E IV Install strategy Explorer JV Install template Strategy template AKTA Explorer 10 5 with Frac 300 AKTA Explorer 10 with Frac 950 AKTA Explorer 10 with Frac 900 AKTA Explorer 10 with Frac 950 AKTA Explorer 100 Air with Frac 300 AKTA Explorer 100 Air with Frac 950 AKTA Explorer 100 with Frac 300 lorer 100 with Frac 350 Have Disk lt Back Nex
16. e Remove Tools gt GoTo menu from Explorer e Remove File menu from Explorer e Remove common programs groups from Start menu e Disable context menus for the taskbar e Disable Explorer s default context menu Make sure all the other options are deselected Expand the Windows NT System item by clicking the plus sign Select these options e Parse Autoexec bat e Disable Task Manager Make sure all the other options are deselected e Click OK e Choose File Save e Close the System Policy Editor Log off Windows 2000 Restrict access in Windows Ex plorer Security 5 When you have edited the system policy as described above the user can still access Windows Explorer in Windows 2000 workstations This can be achieved by clicking the Windows Start button and right clicking on Programs e Therefore you must restrict access to the drives in Windows Explorer Note You do not have to perform this in Windows XP The table below describes how to do this Step Action e Log on to the computer as an administrator Note This can be any user with Windows administrator rights Open your local security policy Result The Group Policy window in displayed Close the Group Policy window and log off from the computer Note The procedure above for group policy changes by default Click Start Run and then type gpedit msc In the tree view select the folder User Configuration gt Administrat ive Temp
17. when connected e Click OK and close the Network Connections window Log off Edit the system The table below describes how to edit the system policy with the System Policy policy Editor Action e Log on to the workstation as the user that will run UNICORN and whose access you want to restrict the same user as in the instructions above Note The user must have Windows administrator rights other wise the System Policy Editor will not be able to save the settings e Double click the shortcut UNICORN Programs Poledit Result The System Policy Editor is started e Choose File Open Registry e Double click the Local User icon ep 103 5 Security 5 5 Single application mode 03 0014 93 ep 104 Step Action Expand the Shell Restrictions item by clicking the plus signs Select these options e Remove Run command from Start menu e Remove folders from Settings on Start menu e Remove Taskbar from Settings on Start menu e Remove Find command from Start menu e Hide all items on desktop Make sure all the other options are deselected Expand the Windows NT Shell Custom folders item by clicking the plus signs Select these options e Custom Programs folder e Hide Start menu subfolders e Custom Startup folder Make sure all the other options are deselected Expand the Restrictions item by clicking the plus sign Select these options e Remove View gt Options menu from Explorer
18. 203 10 1 25 8000 16000 0 8 Technical specifications A How to ensure To ensure maximum resolution for a part of a run issue a New_Chromatogram ae resolu instruction at the beginning of the part This empties the data buffers and resets the sampling frequency to the value specified in the system settings ep 193 03 0014 93 ep 194 A Access groups 90 defined 138 Undefined 138 Access groups edit 140 Access groups view 139 Access items 138 Access security purpose 90 access passwords 90 default user delete 90 Access to folders define 154 Access to systems define 154 Accidental shut down possible reasons 98 avoid 98 Administration areas descriptions 113 Administrator responsibilities table 4 Administrators tasks prepare the program for users 113 Advanced items define 152 define 153 Alarm limit 166 Alarms settings 166 Assign user properties 149 define the advanced items 152 define the advanced items 153 Audit Trail dialog box global view 126 items description 126 system view 127 items 127 Audit trail system setup 164 Audit trails 125 Index epi Index Global view 125 System view 125 print 129 print 129 renew 130 back up 131 display the system run hours 132 Audit trails 134 Audit trails older view 128 Auto save feature 97 i AKTAdesign system sample pump 85 monitor calibration 86 Back up audit trails 131 C Calibrate KTAdesig
19. 950 USB drivers rivers Note This installation is only necessary if you use the CU 950 USB Step Action e Start and log on to the PC e Connect the CU 950 power cable to the mains socket and wait until the LEDs show that it has started up Power LED On steady light PC LED Flashing System LED Flashing e Connect the USB cable to the CU 950 and the PC 2 Since this is the first time you connect the CU 950 the Found New Hardware Wizard is started Found New Hardware Wizard Welcome to the Found New nS Hardware Wizard This wizard helps you install software for CU 950 If your hardware came with an installation CD QP or floppy disk insert it now What do you want the wizard to do Install the software automatically Recommended Install from a list or specific location Advanced Click Next to continue lt Back Cancel e Select the option Install the software automatically e Click the Next button ep3l 2 Installation 2 2 Hardware installation 2 2 3 How to install CU 950 Step Action Found New Hardware Wizard Please choose your search and installation options Search for the best driver in these locations Use the check boxes below to limit or expand the default search which includes local paths and removable media The best driver found will be installed IV Search removable media floppy CD ROM IV Include this location in
20. AKTAxpress Note The instruments that can be used with CU 900 PCI can also be used with the CU 950 See 2 2 3 How to install CU 950 on page 30 for information on how to install CU 950 A maximum of four instruments liquid handling modules can be connected to one PC as specified by the rules below e One instrument can be connected to each CU 900 PCI card e UNICORN supports up to four CU 900 PCI cards on one PC The table below describes the hardware installation i e how to install the CU 900 PCI card in an empty PCI slot in a PC Step Action e Turn off the power to the PC e Remove the power cable from the mains socket e Open the PC cover See the PC documentation if you are not sure how to do this Take the expansion card out of the anti static bag Handle with care Handle the card by its edges and avoid touching the electronic components as far as possible discharges of static electricity can permanently damage electronic components on the card If you are working in a room where static electricity tends to build up discharge any electricity from your body by touching an earthed metal surface for example a water tap or radiator before handling the card e Locate the DIP switch on the expansion card How to do this is described in 2 2 2 How to locate the DIP switch on page 27 e Check the DIP switch setting to determine which CU is selected Write down the number of the selected CU How this is done is
21. Administration 6 6 3 3 How to create a new user Introduction This section describes how to create a new user and assign a home folder for a user s methods and results The default user A default user is created when the system is installed The default user has unrestricted access to all UNICORN functions You log on with this profile when you access a newly installed system for the first time Note The default user should be deleted when regular user profiles have been created The table below describes how to log on as the default user Step Action Start UNICORN e Select user default from the User name drop down list e Type the password default e Click OK Note The default user is the only user where the user name and the password can be identical CE 8 x a UNICORN logon User name default Password Cancel Help ep 145 6 Administration 6 3 User Administration 6 3 3 How to create a new user The User Setup All user administration is performed in the User Setup dialog box in the UNICORN dialog box Manager module It is accessible only to authorized users and the default user e Choose Administration User Setup when you want to display the User Setup dialog box See the illustration below OOOO hl Go Define users and their security attributes m Access groups Each user is assigned to an access group This group defines access to different functions
22. Administration 6 How to assign user properties In the User properties dialog box in the UNICORN Manager module users are assigned different properties that define for example e folders and chromatography systems that the user can access e password rules The table below describes how to open the User properties dialog box Choose Administration User Setup in the UNICORN Manager module Result The User Setup dialog box is displayed e Select a user in the Users list e Click the Edit button Result The User Properties dialog box is displayed Note By default the User item is displayed when the dialog box opens Select another item Attributes Advanced Access or Instructions to view their respective properties Note The dialog box is illustrated in Items of the User properties dialog box below ep 149 6 Administration 6 3 User Administration 6 3 4 How to assign user properties Items of the User The illustration below displays the User properties dialog box with no item selected n dialog ox ICCC j x Dave David Wilson User A Alttibutes Advanced On Access SA Instructions No extended information to display Items The table below lists the items of the User properties dialog box and some of their properties that can be edited Property examples User name password access group User interface and program options Password age account lock
23. Computer Change Names dialog is opened e Select the Domain radio button and write the domain name in the text field e Click OK epl19g9 1 Network setup 1 5 How to configure the workstations 1 5 2 How to configure Windows XP workstations Step Action e Type User name and Password in the subsequent dialog and click OK e Click OK in the Domain Welcome dialog 6 A dialog is displayed which informs that the computer should be restarted for the changes to take effect Click OK in the dialog and restart the computer To map a drive The last steps in the network setup are letter and install UNICORN e to map the shared folder to a drive letter e to install the UNICORN software See the instructions below How to map the Follow the instructions in the table below to map the shared folder to a suitable shared folder drive letter Step Action Log on to the Windows XP workstation with domain user rights This user must also be a member of the user group created before Open Windows Explorer Select Tools Map Network Drive to connect the shared folder on the UNICORN file server to the designated drive letter e Select the drive letter in the upper drop down list box e Make sure the Reconnect at Logon option is selected e Click the Browse button e Locate and select the shared folder UNICORN will use and click OK e Click the Finish button How to install Follow the ins
24. E ENTA EEE EEA E ATEENA 138 6 3 2 ACCESSItE mS aaa e ar E e E Got ANAE 142 6 3 3 How to create a NeW MUSE iss sacevanesvaiminaaieavenvixeunes anne duets tesvaxtovadseareweriwnndns 145 6 3 4 How to assign user PrOPEITIOS sci csees cor ycee Fad hancgandde chdedave fageoaWeyaisaededneteeseans 149 6 3 5 How to change user passwords and user attriDUteS ccceeeseeeeeeeeeee eens 158 6 3 6 How to delete users and Toldersi cc sids sesurcari widleceawk cei iaprniauaaenyacieats 161 TEV EA A cecal oe hie deste ea ccta aes ve lean aise doves A T 163 7 1 The Instructions dialog DON erecta ute vacc ald fay yh tied svag hh wid othe htt leet beh td saan se 164 TAER E EE a A E E E EA A AE A 166 Z3 Specials Seting Sn maea E E E T E AE 169 TA Monitors setting Serisine ng Sane aT E TETUA L EEA E TETEA 170 TE ON EEEE TE A E E E OET 171 7 0 CU 950 settihE Snee a a a a a R a a a ea a ara aTa 172 G TROUDLESNOOUING a raea aae a aaa A aeaa aa a a TAa aa aa aa AEE rA EEDA AAE Tadaa aE 174 8 1 Advice on operation LOGON sentescsswasotteterrnenonteis iaatmenmidetslenseardermierdeiadrasentante dds 175 8 2 Advice on operation UNICORN ACCESS cccceeccsereereeeeeeteeeeeeeeeeeeeeeaaeeneeeneees 177 8 3 Advice on operation MethodS s sssssrsrserrsrrsrrrrrersrrtrrsrrtrsrrrrrerrtrrnrrererrtrrnn 180 8 4 Advice on operation Evaluation ssssssssserssrrsrrrrrersrrtrrsrrtrsrrrrrsrrtrtnrrererrtrrn 184 8 5 Advice on operation KTAdesign SYStOM
25. Montos Mode Disabled HighAlsem 999 99 mS cm LowAlarm 0 00 mS cm Click a radio button to select one of the Instructions groups e Alarms See 7 2 Alarms settings on page 166 for more information e Specials See 7 3 Specials settings on page 169 for more informa tion e Monitors See 7 4 Monitors settings on page 170 for more inform ation e Curves See 7 5 Curves settings on page 171 for more information Result The instructions and parameters for the selected Instructions group is displayed 3 Instructions and parameters In the list e the heading items are instructions e the indented items are parameters 03 0014 93 ep 164 System settings 7 Step Action How to change a parameter setting e Select an instruction or a parameter in the list box e Use the controls to the right in the dialog box to change the asso ciated parameter setting Result The parameter is updated in the list How to restore a parameter to its default setting as defined in the system strategy e Select a parameter in the list e Click the Set Selected Parameter to Strategy Default Value button Result Only the currently selected parameter is returned to its default value How to save the settings e Click OK to save the settings when all the changes have been made Or e Click the Cancel button to discard all the changes made since the dialog box was last opened e p165 7 System settings 7
26. Restart all the UNICORN com puters in the system table e Change the port number in the Socket dialog box e Restart all the UNICORN PCs in the network Note To check which port num bers are busy you can type the command netstat in the Windows Command Prompt window Note If you cannot establish a connection to the network server you can still continue to work from the local station and use the Method Wizard to create methods See the User Reference Manual for more information The Connection field in System Control displays a NO 1 NO 2 or NO 3 Troubleshooting 8 Scenario The Connection field in the Run data pane in System Control says NO 1 or NO 2 The Connection field in the Run data pane in System Control says NO 3 Solution If you use CU 900 PCI Check that the CU 900 PCI is configured according to settings made during the UNICORN installa tion The same Control unit number must be set on the card as in the UNICORN software See 2 2 2 How to install CU 900 PCI on page 26 If you use CU 950 e Choose Administration System Setup in the UNICORN Manager e Select the system with problems in the dialog box and click the Edit button e Check that the strategy computer name and the control unit number are correct according to the installation at the local station which is physically connected to the system See 6 2 1 System defini ti
27. Strategies are installed independently of systems A strategy is assigned to a system when the system is defined or e Click the Next button to continue to the next installation step 03 0014 93 ep 48 Step 11 System Installation Installation 2 The System Installation dialog box is displayed if you chose system installation in Step 4 Select Components System Installation x Select a system and press the CHANGE button to edit the settings for the selected system When all systems physically connected to the computer have been defined press the NEXT button to continue Select System Systemi C System2 C System3 C System 4 System name Strategy Controller Settings Control Unit lt Back Next gt Cancel The table below describes how to define each system that is directly connected to the PC 1 e Click one of the radio buttons to select a system e Click the Change button to set up the system Result The System Setup dialog box is displayed In the System Setup dialog box you set the system parameters for each system connected to the PC one system at a time Continue with the step that correspond to the controller s you will use e CU 950 USB with USB connection see step 3 a below e CU 950 Advanced with Ethernet connection see step 3 b below e CU 900 PCI expansion card see step 3 c below Note It is only possible to have systems with the
28. chromatography control process as such 1 4 Introduction In this section Network setup 1 How to configure the network server This section describes how to configure the UNICORN network server This section contains this topic See Topic How to configure Windows 2000 Server 1 4 1 epg 1 Network setup 1 4 How to configure the network server 1 4 1 How to configure Windows 2000 Server 1 4 1 Introduction How to create a user group and and add users 03 0014 93 ep10 How to configure Windows 2000 Server This subsection describes how to set up a UNICORN network with Windows 2000 domain and TCP IP network protocol Follow the instructions below to create a global user group with a suitable name for example UNICORN Users e members of the user group Step m O N Action Log on to the UNICORN file server with domain administrative rights Select Start Programs Administrative Tools to enter the Active Directory Users and Computers for the domain Create a group that all UNICORN users will be part of Select the Users folder in the tree structure and then Action New Group Enter a suitable name for the group Set Group scope to Global and Group type to Security Click OK Create the users that will run UNICORN Select the Users folder in the tree structure and then Action New User e Type First name Last name and User logon name
29. files Administration 6 Audit trails The audit trail provides the system administrator with a full record of UNICORN usage and system activity There are two types of audit trail files global files and system specific files The global audit trail files e are saved on the server disk in a network installation e are examined via a network connection The system audit trail files e are saved on the local station to which the chromatography system is physically connected e can be examined from the local station without logging on to the network e can be examined from any computer in a network installation The Audit trail dialog box has two or more tabs one Global tab and a System tab for each installed system e The global tab displays usage information for the complete UNICORN installation e The system tab s displays usage information for a chosen system The table below describes how to examine the global and system audit trail files Step Action Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box is opened see the illustration in 6 2 2 The Global tab on page 127 By default the Global tab is displayed which shows the information of the global audit trail file If you want to examine the audit trail file for a system click a System tab There is one tab for each installed system See illustration in 6 2 2 The System tab on page 128 Select the chec
30. in UNICORN When changing a group all users assigned to that group are affected Users Name Full name Group default default Administrator OPC user OPC user Administrator i Instruction The table below describes how to create a new user Step Action Click the New button in the User Setup dialog box Result The Create New User dialog box opens See illustration below this table e Type a user name in the User name text box e Type the full name of the user in the Full name text box e Type the position of the user in the Position text box 03 0014 93 ep 146 Administration 6 Step Action Select a Home folder e Select a drive from the Drive drop down list and a folder from the Name drop down list e Go to step 6 Create a Home folder e Ifyou need to create a new home folder go to step 4 See Home folders below this table for more information Click the New button Result The Create New Folder dialog box opens e Select a drive and type a folder name e Click OK Result The folder is created and you return to the Create New User dialog box Click OK Result The new user is created and added to the Users list of the User Setup dialog box Repeat step 1 6 if you want to create more users Click the Close button The Create New The illustration below displays the Create New User dialog box User dialog box Create New User x
31. installing UNICORN for the first time on the computer The Select Sub components dialog box Select User Information check box and click the Change button Result The Select Sub components dialog box is dis played showing the sub components e Global Procedures e Global Report Formats e Global BufferPrep Recipes e Global Columns e User File Select the components you wish to install If sub components already exist If any of the above components already exist from a previous installation you will be asked if you want to replace them with the new default files e Normally you should answer No and keep the ex isting files Note See also 2 3 3 User Information on page 69 where this dialog box is described in more detail Strategy and Template Select the Strategy and Template files check box to files install strategies and templates e Click the Change button to display the Select Sub components dialog box There you can select which components to install Strategy files and or Tem plate files System installation The System check box must be selected for worksta tions where systems are connected but not on demo stations Manuals e Select the Manuals check box if you want to install the UNICORN manuals e Click the Change button to display the Select Sub components dialog box There you select additional system manuals to install e When you have selected the components click the Next button
32. item UNICORN will not allow you to remove this access item from all access groups e UNICORN Manager If access to the UNICORN Manager is restricted for an access group the access group will still be able to Log off Quit program Change user attributes Change password e p141 6 Administration 6 3 User Administration 6 3 2 Access items 6 3 2 Access items What is an access An access item specifies a specific action that the user is allowed to perform in the 1em UNICORN system The access items are assigned to one or more user access y 8 group s in the User properties dialog box see 6 3 1 User access groups on page 138 To view the access items e choose Administration User Setup in the UNICORN Manager and click the Access groups button Result The access items are listed in the Access field when the access group is selected Description of the The table below describes each access item access items Access item Allows the user UNICORN Manager to create and edit methods with the UNICORN Manager Method Editor to create and edit methods for pre programmed con trol of systems with the Method Editor module System Control to control and monitor processes online with the System Control module Evaluation to process result data with the Evaluation module User setup Groups to define and change access levels and users Caution It is recommended that only one user in an installation or
33. network with a Windows server and TCP IP network protocol All users must have the Windows user right Access this computer from the network which is also the default user right If the user right is not correct the network administrator can change it Follow the instructions in the table below to set the user right to Access this computer from the network Step Action Log on to the workstation with administrative rights Choose Start Control Panel Administrative Tools Local Security Policy Note If you cannot see the individual items in the Control Panel you have to click the link Switch to Classic View in the left pane of the Control Panel Choose Security Settings Local Policies User Rights Assignment in the tree structure Double click the Access this computer from the network option Click the Add User or Group button Result The Select Users or Groups dialog box is opened Select Users or Groups Select this object type Users or Built in security principals Object Types From this location Enter the object names to select examples _ Make sure the correct domain is displayed in the text field From this location Click the button e Object Types to change the type of objects to select e Locations to change the root location domain from which to begin your search epl7 1 Network setup 1 5 How to configure the workstations 1 5 2 How to configure Windows
34. of the local computer one at a time in order to restore the local policies on their accounts as well Note Make sure to log on as a user in step 1 and 2 03 0014 93 ep 108 Security 5 5 6 The Options dialog box Introduction This section describes the Options dialog box in the UNICORN Manager How to open the In the UNICORN Manager choose Administration Options to open the Options dialog box dialog box Te x Global T Run UNICOF m Evaluation I Show negative retentions Asymmetry Ratio at fi 0 0 100 of peak height Resolution algorithm e Oligo IV Start message IV Sequence check J Sequence paste OPC J Logon Logoff security HDA Memory cache limit P AllUsers 0 I Take Control HDA File cache limit 10 HDA File cache path TEMP Browse Cancel Help There are four fields in the dialog box which are described individually below The Global field Select the Run UNICORN in single application mode check box to activate UNICORN single application mode on the computer Explanation Single application mode means that UNICORN will be the only application available when it is running The setting is done on a per computer basis and will apply to everyone who starts UNICORN on the specific computer To achieve single application mode UNICORN is started in a separate desktop When UNICORN is terminated Windows switches back to the ori
35. point calibration is based on measurements at two reference points The ton monitor will be calibrated assuming a linear response between the two reference values It is important that monitors are calibrated in the measuring range for which they will be used particularly when the response is not linear over the whole operating range of the monitor see the figures below r rd Pi calibrated a 2 mmm r response P actual actual og response working range response j calibrated J f 7 response y Ca Good calibration Poor calibration Note For a monitor with a non linear response make sure the reference points are within a linear working range Continuous calib Continuous calibration is based on a value accumulated during a given time This ration kind of calibration applies to the sample pump in KTAdesign systems e p85 4 Calibration 4 2 How to calibrate monitors for AKTAdesign systems 4 2 How to calibrate monitors How to calibrate a pH monitor 03 0014 93 e p 86 How to calibrate monitors for KTAdesign systems The table below describes how to calibrate the monitors for KTAdesign systems Step Action Select System Calibrate in the System Control module Result The Calibration dialog box is opened Select a monitor in the Monitor drop down box and perform the procedures as described in the dialog box Repeat step 2 for each monitor type Click th
36. processes including those needed for system connection The services are only started when the computer is booted up Print screen does not send a copy of Scenario Solution the screen to the f i printer The Print screen command only makes If you want to print the view on the a copy of the screen to the clipboard screen press the lt Print Screen gt key and not to the default printer and paste the image from the clip board into an appropriate program such as Microsoft Paint and then print out the image ep 183 8 Troubleshooting 8 4 Advice on operation Evaluation 8 4 Advice on operation Evaluation In this section This section describes the following evaluation scenarios e Incorrect date and time in the result file e Evaluation procedure aborts Incorrect date and time in the result Scenario Solution file 7 The result file shows incorrect date The date and time recorded in the and time result file are taken from the PC sys tem clock settings Action Check the system clock set tings Evaluation proced J ure aborts Scenario Solution The evaluation procedure aborts Instructions in an evaluation proced ure refer to curves by identification number irrespective of the curve names Make sure that the curves processed when the procedure is ex ecuted are compatible with those pro cessed when it was recorded An eval uation procedur
37. resolutions at 10 Hz initial frequency 192 ensure maximum resolution 193 Data storage 192 Default system parameters 164 Default user 145 log on instruction 145 Default user delete 90 Delete a folder 161 Delete a user instruction 161 Delete special folders instruction 162 Delete users and folders 161 Disconnect scouting run 81 MethodQueue 81 Disconnect system 81 Display the system run hours 132 E Edit the user definition instruction 151 Edit the user definition 151 03 0014 93 epiv Enable alarms 167 Examine global audit trails 125 F Failed folder retreive the results 93 policies for access 94 access policies 94 Folder to which several users share access delete 162 Folder delete 161 Fraction collector Specials settings 169 G Global audit trails examine 125 Global BufferPrep recipes 68 Global procedures 68 Global report formats 68 H Hardware installation neccessary when 25 CU 900 PCI expansion card instructions 26 CU 950 instructions 30 Hardware recommendations 189 Home folder create subfolders 148 delete 162 Home folders network installations 148 non network installations 148 Hysteresis setting 168 Index epv Index Install components 67 Install software 67 Install system 71 overview 23 system recommendations 23 Software 36 Prerequisites 36 Software license agreement 38 Strategy files 47 Template files 47 Software 54 Prerequisit
38. same type of controller on one physical computer either CU 900 PCI or CU 950 ep 49 2 Installation 2 3 Software Installation 2 3 1 How to install UNICORN Step Action 3a System Setup for CU 950 USB System Setup xj Set the system parameters for the selected system Set the system name type and the strategy that shall be used for the system Select and setup the installed type of controller System name System 1 System type Chromatography KTA bi Strategy E100F400 hd Controller Settings CU 950 USB Control Unit fi CU 950 Advanced CU 900 PCI Help Default Clear e In the Controller field select the CU 950 USB option e Type anew System name if you want to change the suggested name e Choose the System type and Strategy e Choose the Control Unit number 1 Note The number must be set to 1 when you use CU 950 USB since only one such unit can be connected and it must have ID 1 e The Default button sets the default resource settings for the selected system type e The Clear button clears all fields if you want to remove a system e When you are finished click OK 03 0014 93 ep 50 Installation 2 Step Action System Setup for CU 950 Advanced System Setup i x Set the system parameters for the selected system Set the system name type and the strategy that shall be used for the system Select and setup the installed t
39. the chromatogram curves 1 46 to display in Quick view Example The value 1 displays the first chromatogram curve which is Indicates the height of each fraction Indicates the height of each injection Logbook mark height Indicates the height of each logbook mark in a chromatogram The Advanced Open the User properties dialog box see the table in the beginning of this section item User Attributes Advanced 3 Om Access BA Instructions and select the Advanced item User properties eC Dave David Wilson Advanced Password age I Expires in F 0 days Password uniqueness I After g bad logons Automatic workstation I Lock after ayes I Logoff after r Signature Password LK ce e x e p 153 6 Administration 6 3 User Administration 6 3 4 How to assign user properties Advances properties The table below describes the Advanced properties Property Description Password age The number of days a password is valid 14 182 Password uniqueness How many times a password must be changed before the same password can be used again 1 1024 Account lockout How many bad logins that are allowed before the account is locked 1 20 Automatic workstation How many minutes of inactivity before the worksta lock logoff tion is locked or the user is logged off 1 480 Signature An additional password Note This cannot be the same as t
40. the search JENDrivers Cu950 gt Browse Don t search will choose the driver to install Choose this option to select the device driver from a list Windows does not guarantee that the driver you choose will be the best match for your hardware cmo e Select Search for the best driver in these locations e Select only Include this location in the search e Insert the UNICORN CD into the CD ROM drive of the PC e Click the Browse button and locate the folder Drivers Cu950 e Click the Next button Result The necessary files are copied to the hard disk Note In Windows XP a warning may be displayed saying that the drivers are not signed Choose the option to install anyway The Completing the Found New Hardware Wizard screen is displayed e Click the Finish button to complete the installation 03 0014 93 ep 32 Installation 2 CU 950 system To be able to control a system an instrument with the CU 950 controller the installation UNICORN software has to be set up accordingly Usually this is done when the UNICORN software is installed for the first time but it can also be done afterwards System installation during UNICORN software installation The system installation part of the UNICORN software installation is described in 2 3 1 Step 11 System Installation on page 53 System installation after UNICORN software installation The table below describes how to perform a CU 950 system in
41. the user right to Access this computer from the network Step Action Log on to the workstation with administrative rights 2 Select Start Settings Control Panel Administrative Tools Local Security Policy 3 Select Security Settings Local Policies User Rights Assignment in the tree structure Double click the Access this computer from the network option Click the Add button and make sure the correct domain is displayed in the top drop down menu Select the group or person s who will receive this right and click the Add button Click OK In order to connect a Windows 2000 workstation to the network the following network components need to be installed on the workstation e Client for Microsoft Networks e File and Printer Sharing for Microsoft Networks e TCP IP Protocol e A valid network card ep 13 1 Network setup 1 5 How to configure the workstations 1 5 1 How to configure Windows 2000 workstations How to install Follow the instructions below to install the necessary network components network compon ents Step Action e Start the computer with the appropriate network card inserted e Log on to the Windows 2000 workstation with administrator rights The adapter will be found and installed Choose Start Settings Control Panel Double click the Network and Dial up Connections icon Double click the Local Area Connection icon Choose Properties and then Install If the component Cl
42. to a backup location Since access restrictions are in place you can do it like this Open the Windows Command Prompt e Inthe Command Prompt window type copy c winnt sys tem32 grouppolicy user registry pol a tocopythe file to a floppy disk Note lt user gt is the identity you used to log on in step 1 Open the local security policy e Click Start Run and then type gpedit msc Result The Group Policy window in displayed e In the tree view select the folder User Configuration gt Administrat ive Templates gt Windows Components gt Windows Explorer e Inthe right window pane double click the setting Prevent access to drives in My Computer e Click the Policy tab e Click the Disable radio button e Click the Apply button and then OK e Close the Group Policy window e Copy the backup Registry pol file created in step 2 back to the folder C WINNT System32 GroupPolicy lt user gt Since access restrictions are in place you can do it like this Open the Windows Command Prompt window Type copy a registry pol c winnt sys tem32 grouppolicy user to copy the file from a floppy disk e When prompted to replace the existing file click Yes Security 5 Step Action Log off from the computer e Log on to the computer again as the same administrator e Verify that the restrictions no longer apply to the administrator Log off from the computer e Log on to the computer as ano
43. to continue e p 57 2 Installation 2 3 Software Installation 2 3 2 How to install UNICORN for AKTAxpress Step 5 Select In the Select Drive dialog box you choose the installation folder for the UNICORN software Drive Select Drive x The UNICORN software have to be installed to a folder named Unicorn located in the root directory of the selected drive Select the drive that you want to install the UNICORN software to and press the NEXT button to continue Available disk drives on the system Destination Path C UNICORN a Follow the instructions in the table to select a disk drive Step Action Select the disk drive where the program is to be installed This should be a physical disk drive usually C on the computer where you in stall UNICORN not a network disk drive e Click the Next button to continue e Click the Yes button if asked whether Setup should create the UNICORN program folder 03 0014 93 ep 58 Installation 2 Step 6 Setup The Setup Type dialog box is displayed Type Setup Type x Select the type of installation you prefer then press NEXT The network installation will setup the software to work over a network Check Remote only system if no systems will be physically connected to the computer 4 remote only system have to be installed to an existing UNICORN server The stand alone installation will setup the software to work local
44. 0 Index ep vii Index map shared folder instructions 20 install Unicorn instructions 20 Network setup using TCP IP and Windows NT create user groups and users 10 Network terms about 4 explanations table 5 storage of data 5 communication 5 log files in a network setup 5 named pipes communication 5 network failiure in a middle of arun 5 access to the network drive while running 5 server in a Unicorn network setup 5 P Parameters curves settings 171 Passwords rules and recommendations 158 pH monitor calibration 84 Prepare UNICORN for usage instruction 113 Print audit trails 129 Print audit trails 129 Program files 68 Q Quit 82 R Remote connection 75 Remote workstation definition 4 Remote only systems 71 03 0014 93 ep viii Renew audit trails 130 Resolution at 10 Hz initial sampling frequency 192 Result file transfered 97 S Sampling frequency 192 Security access groups 90 Select components 67 Select Sub components 68 Settings alarms 166 specials 169 monitors 170 curves 171 CU 950 172 Simultaneous connections 77 Software installation 67 Software components Select at installation 39 Select at installation 56 Software recommendations 190 Software recommendations 190 Special folders delete 162 Specials settings 169 Stand alone installation 74 Storage 192 Store monitor signals 171 Strategy files Installation 47 Installation 63
45. 0014 93 e p16 Select the drive letter in the upper drop down list box Make sure the Reconnect at Logon option is selected Click the Browse button Locate and select the shared folder UNICORN will use and click OK Click the Finish button Follow the instructions in the table below to install UNICORN Step Action Install UNICORN See 2 3 1 How to install UNICORN on page 36 select the Network installation option or 2 3 2 How to install UNICORN for AKTAxpress on page 54 Reboot the PC and log on as one of the domain users that will run UNICORN Connect the shared folder again This is necessary since connected network drives are user specific Open Windows Explorer Select Tools Map Network Drive to connect the shared folder on the UNICORN file server to the designated drive letter Select the drive letter in the upper drop down list box Make sure the Reconnect at Logon option is selected Click the Browse button Locate and select the shared folder UNICORN will use and click OK Click the Finish button Start UNICORN and set up the system definitions see 6 2 1 System definitions on page 115 the user profiles see 6 3 User administration on page 137 1 5 2 Introduction User rights How to set the correct user right Network setup 1 How to configure Windows XP workstations This subsection describes how to configure Windows XP workstations in a UNICORN
46. 1 Do not copy the UNICORN is supplied on a CD ROM Files on the CD ROM are compressed and Hee o cannot simply be copied onto the hard disk During the installation procedure the required folder structure is created on the hard disk and the files are decompressed Do not attempt to decompress the files using any other file decompression utility Step 1 Insert the Follow the instructions in the table below to begin the installation Setup CD Step Action e Insert the CD ROM disk into the CD ROM drive The UNICORN Setup Program should start automatically If not e click the Windows Start button and select Run e type the command d setup where d is the unit for your CD ROM drive e click OK The UNICORN Setup program is launched Continue the setup be low 03 0014 93 ep54 Installation 2 Step 2 License The table below describes how to complete step 2 of the UNICORN Setup program agreement and user information e The Welcome dialog box is displayed e Click the Next button to continue e The UNICORN Software License Agreement dialog box is displayed You must accept the license agreement to install UNICORN e Click the Yes button to continue e The User Information dialog box is displayed Type your name company and the product serial number of the software The serial number can be found on the UNICORN License Agreement that is shipped with the CD e Click the Next button
47. 2 Alarms settings 7 2 Introduction Alarm and Warn ing limits Messages are dis played on all sta tions 03 0014 93 ep 166 Alarms settings This section describes the Alarms settings which are available in the Instructions dialog box in the System Control module x Instructions Alarm_UV1 Parameters Alaims Mode HighAlaim 25 0000 mALJ C Disabled Enabled C Specials LowAlarm 0 0000 mAU j Alarm_Cond HighAlaem 8000 0000 6000 0000 c Mode Disabled fs000 CS SELES HighAlairn 999 99 mS cm Leg a Pring 0 00 mS cm LowsAlarm 8000 0000 6000 0000 Mode Disabled o 0000 maU High larm 14 000 5 C Curves I mart ann th zl Set Selected Parameter To Strategy Default Value Lock ce eo Note How to open and use the Instructions dialog box is described in 7 1 The Instructions dialog box on page 164 The Alarms settings define e upper and lower warning limits e upper and lower alarm limits for process monitor signals Alarm limits If the signal exceeds the Alarm limits e an alarm sounds e an alarm message is displayed e the process is paused that is the method execution is suspended and all pumps are stopped Warning limits If the signal exceeds the Warning limits a warning message is issued without interrupting the process Alarm and warning messages are displayed on all stations with a connection to the system concerned regardless of the identity and access rights of th
48. 2001091700 Monday September 17 2001 9 2001092400 Monday September 24 2001 2001100100 Monday October 01 2001 2001100800 Monday October 08 2001 2001101600 Tuesday October 16 2001 E 2001102300 Tuesday October 23 2001 9 2001103000 Tuesday October 30 2001 2001110600 Tuesday November 06 2001 2 2001111300 Tuesday November 13 2001 annann z owe 1 AR aan Browse Gk Cancel Help Boe ea Helo e Choose Global or a System in the Type drop down box e Select the file s to open from the File list Use the CTRL key if you want to open several audit trail files at the same time Click the Browse button if you want to open a file which is not listed Note Files are named by date and serial number e Click OK Result The audit trail file s is now displayed on a new temporary tab 03 0014 93 ep 128 Administration 6 How to search for The table below describes how to search for text in audit trail files text in audit trail files Step Action Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box is displayed If you want search the currently open audit trail file e press the CTRL F keys or choose Edit Find in current log Note The currently open audit trail is the one displayed in the Audit trail dialog box and depends on the selected tab Global or a System type the text string you want to find in the
49. 6 15 W Europe Daytght Time Eroe Controle Board 1 error 2012 No response i gt Infcamas 25 04 2002 08 27 17 W Europe Daylight Time Eirce Frac 950 0 enor 62 DiopSyne Not Conn I Timezone The table below describes the items which can be displayed on a System tab Item Displays Run End Manual e the times for start and completion of a run e the times for manual operation Alarms Warnings alarms and warnings defined in the strategy for the system Calibration monitor calibration operations ep127 6 Administration 6 2 System administration 6 2 2 Audit trails Item Displays Information system log started stopped Timezone the time zone where the activity was logged How to view tll A new audit trail file is created at regular intervals and the old audit trail file is ous audit trail files automatically saved See 6 2 2 How to renew audit trail files on page 131 for more information The table below describes how to view previous audit trail files Action Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box displays the current audit trail e Choose File Open to display the Open audit trail dialog box Open audit trail xi Type File z Name _Date 9 2001082000 Monday August 20 2001 2 2001082700 Monday August 27 2001 9 2001090300 Monday September 03 2001 2001091000 Monday September 10 2001 2
50. Advanced 03 0014 93 ep 30 How to install CU 950 The CU 950 controller is an interface to connect chromatography or synthesis instruments to a PC CU 950 can be used together with all the AKTA instruments except AKTAprime and AKTAxpress Note The instruments that can be used with CU 950 can also be used with the CU 900 PCI expansion card See 2 2 2 How to install CU 900 PCI on page 26 for information on how to install CU 900 PCI There are three LEDs on the front of the CU 950 which can be in three different states e Off no light e Flashing e On steady light The table below describes what the state of each LED means Flashing On LED Off Power Power off Power on BIT Power on BIT running or BIT OK not OK PC Power off Power on no PC Contact with PC communication established System Power off Power on no sys Contact with sys tem AKTA in tem AKTA in strument commu strument estab nication lished BIT Built in test There are two versions of the CU 950 controller USB and Advanced The table below describes how they connect and how many that can be connected to the PC Controller Connector Max units connected CU 950 USB USB Universal Serial 1 Bus CU 950 Advanced Ethernet 4 In order to use the CU 950 USB certain drivers need to be installed See instructions below Installation 2 coe install the The table below describes how to install the CU
51. CCSSSCCUNILY cca sate antes menu T dco tme tours tnas teuss cazne gr eaeieaebaneta went t bana nate 90 52 CONMEC TION SCCUMILY irc ea ve Ea aE a nese honda A E E AAEN 91 Boa Datel La TETEE E A Secwunseateaisaiaenetotan 92 5 3 1 Network communication failure remote Station ccccccceeeeeeneeeeeeeeeeaeeeees 93 5 3 2 Network communication failure local Station ccccecceeeeeeeeeeeeeeeeeeeeaeeeees 95 Boos bOCal station TAMU sir keen calcd a a a iia v tases a p eE 96 Table of Contents 5 4 How to prevent accidental SHULCOWM sta tcccnncterrsecatertedaesan wut heeasteanenteresvatendaisd siete 98 5 5 Single application MOOS cc stinunt acatervenouds dhe ies teeees casa dereebi ht cameutehontssataees 99 5 6 The Options dialog OK aviv ctac ta cuinccde eet nati ue tatiesGce ates ntinccnetonenmeat annette tranes 109 Gs PRIMI SEL AMON EEE E aveiunesnatsvesayseaye socdwte EE 112 6 1 Administration OVEIVIEW aisiacncs seacavenherbiridvenienvarennsastuateradiuaracenRariabrenueds 113 6 2 System administration isisi iieininandai oidini ninina inan adnada aaea e andaina as daaa a aanraai dinana 114 6 2 lo System definition Sace a a E a a E a 115 6272 Audit Mall Sie n a aa e alae N A E A 125 6 2 3 How to back up and restore system definitionS sssssrsreerrsrerrrrrerrsrrerre 134 6 3 User AMMIMSWAt ON ssccscieciceccdeescesceceveesaecsctecudesenccedcenesdadeunedecccussencsandedesueeteauebenaeandien 137 6 3 User access TOU DS ee a Ea
52. Find Text dialog box and click the Find button e press the F3 key or choose Edit Find next to find the next occur rence of the search string If you want to search all the existing audit trail files either Global or System e choose Edit Find in log files e type the search string choose whether to search Global or System audit trail files in the Files at drop down box and click the Find button zi Text Files at Globa o Global d j ce Result The search results are displayed on a new temporary tab named Find result How to print The table below describes how to print an audit trail file audit trails Step Action Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box is displayed e p 129 6 Administration 6 2 System administration 6 2 2 Audit trails 2 e Select the Global tab or a System tab e If you want to print other audit trail files than the currently open then open the file s as described in How to view older audit trail files above e Choose File Print to print the file s displayed in the dialog box How to renew The audit trail file is renewed at regular intervals between 1 to 30 days The old audit trail files audit trail file is then saved automatically together with all the previous audit trail files The table below describes how to set the renewal interval and how to renew the audit trail immediately Step Action
53. S cccccccccccscesessssseteeeeeesescenerstssananees 185 8 6 Advice on operation CU 950 s ss sssssrsrrerrsrttrrrrrtrsrrtrtsrttrnrrtrtanntrt nrn tann trenn 186 Az Technical specif CANO Sarrar r Trae aera E aa a aaa aana en aer na aana a Adara me hina harena aaiae 188 A 1 System FECOMMENCALIONSy 60 couse shrnndsdeordaiae mnie alemunaiatr nena EEEE EEEn 189 AZ UNICORN Control CADAC II ieminaiiredomcedrentoinatedesecotesredeshaniseeratveitenblansalnehnageen 191 A Data Sani Pili evita seston vnc a a a dave a a ange acscuas vhe hacen ikea 192 03 0014 93 epii Introduction In this chapter Network setup 1 Network setup With UNICORN installed in a network environment chromatography systems can be controlled from any PC in the network which has the UNICORN software installed This chapter describes how to set up the network environment of a UNICORN network This chapter contains these sections Topic See Network terms and concepts Network environment UNICORN network example How to configure the network server How to configure the workstations 1 5 ep3 1 Network setup 1 1 Network terms and concepts 1 1 Network terms and concepts Introduction In this section some important network terms and concepts are explained Administrator cat The administrator duties can be divided into two categories with different egories and duties responsibilities e Network administrator Responsib
54. Step 3 Select Sys In the Select System Type dialog box you choose the type of system you will use tem Type an AKTAxpress system or a UNICORN system other than AKTAxpress Select System Type p x Please select the system type to install UNICORN for KTA System except those listed below Ettan LC System AKTAxpress System AKTAoligopilot System BioProcess System p _ amp lt Back Next gt Cancel e Click the KTAxpress System button to continue e p 55 2 Installation 2 3 Software Installation 2 3 2 How to install UNICORN for AKTAxpress Step 4 Select In the Select Components dialog box you choose the components to install by Components selecting the appropriate check boxes Normally all the check boxes should be selected Select Components l x Select the components you want to install clear the components you do not want to install Components Program Files User Information Strategy and Template files OK System Installation 50K This component includes the UNICORN Eh application files Space Required 5I410K Space Available 1558180 K lt Back Next gt Cancel Select the components to install according to the table below Program Files The Program Files check box must be selected 03 0014 93 p 56 Installation 2 Component Description User Information The User Information check box must be selected if you are
55. UNICORN Setup e Click the Finish button to exit the setup program and automatically restart the computer 03 0014 93 ep 66 2 3 3 When to install se lected components How to install se lected components Installation 2 How to install selected software components The installation program can be used to re install selected components of the software It can be useful e if your UNICORN installation is damaged for example due to accidental file deletion or hard disk failure e if you want to install additional systems strategies templates or manuals The table below describes how to install selected software components The specific installation options related to each component are described separately further on in this section Step Action e Close all programs on your computer e Log on to the network if you have a network installation Insert the UNICORN installation CD Click the Next button when the Welcome dialog box is displayed Result The Select Components dialog box is displayed The illustration below is an example of the Select Components dialog box The installation program has detected the previously installed UNICORN files on the computer and suggests the components to install by selecting the appropriate check boxes Select Components xi Select the components you want to install clear the components you do not want to install Components Program Files User Inf
56. XP workstations Step Action 6 Choose one of the following options Option 1 Write the name 1 Write the name of the group or user s in the text field Enter the object names to select examples 2 Click the Check Names button to check if the name is valid 3 If the name is accepted click OK and then OK again Option 2 Choose the name from a list 1 Click the Advanced button An expanded Select Users or Groups dialog box is opened 2 Click the Find Now button to display a list of possible names 3 Select one or more names and click OK 4 Click OK and then OK again Network a In order to connect a Windows XP workstation to the network the following SAS FOES tA network components need to be installed on the workstation e Client for Microsoft Networks e File and Printer Sharing for Microsoft Networks e TCP IP Protocol e A valid network card How to install Follow the instructions below to install the necessary network components network compon ents Step Action e Start the computer with the appropriate network card inserted e Logon tothe Windows XP workstation with administrator rights The adapter will be found and installed Choose Start Control Panel Network Connections Result The Network Connections window is opened Right click the Local Area Connection icon and choose Properties Result The Local Area Connection Properties dialog box is opened and the General tab
57. al station and a CU 950 Advanced control unit during a method run If the network The table below describes what happens when these conditions are fulfilled communication fails e A CU 950 Advanced controller with a memory card is used e Data recovery ON e Method behaviour CONTINUE Note Data recovery and Method behaviour is described in 7 6 CU 950 settings on page 172 e The network communication is lost between the local station and the CU 950 Action e The run continues under the control of CU 950 during the com munication loss e The run data is saved in the CU 950 memory card When communication is reestablished e UNICORN requests the missing data from the CU 950 e the missing data is uploaded to the local station e during the upload the message Uploading is displayed under Instru ments in the Run Data pane of the System Control module The run continues as normal e p95 5 Security 5 3 Data security 5 3 3 Local station failure 5 3 3 Local station failure In this section This section describes what happens if a local station fails during a method run The run may continue but the results generated after the failure cannot be saved unless a CU 950 Advanced controller is used Systems with CU The table below describes what happens when these conditions are fulfilled 950 Advanced i controller e A CU 950 Advanced controller with a memory card is used e Data recovery ON e Method beh
58. alled with the option Remote Only Remote station Network terms In the table below are explanations for some terms which are important to understand when working with UNICORN in a network Term Storage of data Communication Log files in a network setup Named pipes communication Network failure in the middle of a run Access to the network drive while running Explanation Methods and log files are stored in a folder shared between the local and the remote UNICORN The local and the remote UNICORN use either named pipes or sockets to send commands and data between them While running the local UNICORN system writes logs on the local hard drive When the run is over it copies them to the network drive where log files are stored From the remote UNICORN com mands are sent e g run method foo met From the local UNICORN messages and trend data are sent to the remote UNICORN The local UNICORN will continue the run and store log file on the local hard drive when the run is over When a method is started it is copied from the network drive to a local dir ectory During the run the method is read from the local directory ep5 1 Network setup 1 1 Network terms and concepts Term Explanation Server in a UNICORN network setup UNICORN requires a directory for log files and method templates to be accessible by both the local and the remote UNICORN It is generally a
59. ation and later want to connect the system to a network you must remove the current installation and install the software with the ap propriate settings e Select the Demo system check box if you want to install a demo station e Click the Next button Step 7 Program Follow the instructions in the table below to set the required parameters for Options 03 0014 93 ep 60 password and system control layout Program Options x Set the requirement for minimum number of password characters and the System Control layout Press the NEXT button to continue Password Password required Minimum number of password characters 3 i No password required System Control Layout Standard Number of System Control windows fi E AKTAxpress lt Back Cancel Installation 2 Step Action Select Password required and type the minimum number of characters required for passwords Valid numbers of password characters are 3 15 Select No password required if you do not require password protec tion With this setting users can be defined with or without pass words Choose either of the System Control Layout options e Standard The standard layout for the System Control module Must be chosen if Scouting or Methodqueues are going to be used Select the number of system control windows 1 4 AKTAxpress The new layout for the System Control module It has only one Syst
60. aviour CONTINUE Note Data recovery and Method behaviour is described in 7 6 CU 950 settings on page 172 e The communication is lost between the local station and the CU 950 due to a computer crash Action e The run continues under the control of CU 950 during the com munication loss e The run data is saved in the CU 950 memory card When the local station is up and running and the connection between the local station and the CU 950 has been reestablished e UNICORN uploads backup files from the hard drive of the local station e UNICORN requests the missing data from the CU 950 e the missing data is uploaded to the local station e during the upload the message Uploading is displayed under Instru ments in the Run Data pane of the System Control module The run continues as normal 03 0014 93 ep 96 Systems without CU 950 Advanced controller If the local station is not logged on Security 5 When the local station is restarted after the failure the temporary result file will be transferred to either of the following locations e the original result file destination usually on a network drive or e the Failed folder if the original destination is on a network drive which is not available If C is the UNICORN installation drive the path to the Failed folder is C Unicorn Local Fil Failed The result file will contain the results of the run up to and including the last auto save time see
61. control of the system when OPC starts 03 0014 93 ep110 Security Command name HDA Memory cache limit HDA File cache limit HDA File cache path Function Limits Historical Data Access in memory cache to the specified number of bytes Range 0 1 000 000 000 bytes 1 100 gt 100 bytes minimum cache size 0 unlimited cache size Indicates maximum number of result files in cache Range 0 100 0 cache not used The folder where cached result files are stored e p111 6 Administration 6 Administration Introduction This chapter describes the administration aspects of a UNICORN installation for example how to create system definitions and how to assign different access levels to the users In this chapter This chapter contains these sections Topic See Administration overview System administration User administration 63 03 0014 93 ep 112 Administration 6 6 1 Administration overview Administration The table below describes the three main areas of UNICORN administration areas Area Concerns System administration maintenance of software aspects of UNICORN including definition of connected systems see 6 2 1 Sys tem definitions on page 115 monitoring of system usage audit trails see 6 2 2 Audit trails on page 125 e routine monitor calibration see 4 Calibration on page 83 User administration authorization of acce
62. coranmnbeentwaniuraudutseniund aedauanteyats aanata dite ts 25 2 2 2 How to install CU 900 PC lil 2 3 ds ict voces sade evens cece bacruaee tua msheneranessaouces 26 2 2 3 HoW to install GUO 507 ree a a E EA A E 30 2 3 Software Installation saisies aitaan pean aa raaa raara ap suctiunSasausuenstsusdadadewisitannsoance 35 2 3 1 How to install UNICORN sssssssssssssssssressrssrrsrrrsrrrrrrnrrrsrrssrrsrrssrrsrrsrrrerrrnrs 36 2 3 2 How to install UNICORN for AKTAXDpreSS cccccsescescsscseesessssssenceneenenessens 54 2 3 3 How to install selected software components s sssserssrsererrrersrrrrrsrrererrrene 67 3 System CONNECHONS ia a Ai eal nen ten ec ec 73 SLs SYSTEM connection TACTS Hi cainetscavicnston a sevtecdmcatircedumuneidetansabes NE ETEEN EAE rene 74 3 2 How TOSCONMECTASSY SION Ic hucastveys sca candaseontasadarstactagndieananarddebyansiace tas ynesanodechana cues 75 3 3 CORMECTIOM Modes ireen E a cite rie ee tated E E Meee crate ci 77 3 4 How to leave and lock a SVSTCIN ywccuwws uacheen ctv vinniveaw usa sieguryianuniieee uwuuuvorarewnnener 79 3 5 How to disconnect a SVSLE MMi cz xnseris cre dhan and daemons van utanadinneneath Raveena 81 A CalibtatiOnisaniccvsdsir nie anneninnin dna ages 83 4 le Calibration tatis asninn aaea ca edie wen ETE tl A a a EA 84 4 2 How to calibrate monitors for KTAdesign SYStEMS cccccccsccceeeececessessssneeneesens 86 OD SOC UN E E ie An ita aok anise eae aual E AEA 89 S P
63. d 60327 K Space Available 1123892 K e Select the manuals you wish to install e Click the Continue button to return to the Select Components dialog box System connections 3 3 System connections Introduction This chapter describes how to manage system connections between a computer and chromatography systems Note This section does not describe how to manage AKTAxpress system connections For information on AKTAxpress system connections see the AKTAxpress User Reference Manual In this chapter This chapter contains these topics Topic See System connection facts How to connect a system How to leave and lock a system How to disconnect a system 3 5 e p73 3 System connections 3 1 System connection facts 3 1 System control windows Connection man agement 03 0014 93 e p74 System connection facts UNICORN installed on a given computer may have up to four System Control windows The actual number of windows is determined when the software is installed Each window may be connected to one chromatography system at a time Note A network installation may have more than four systems in total but each computer in the network can establish a maximum of four connections Connections are managed with these menu commands from the System Control e System Connect or the System Connect toolbar icon e System Disconnect or the System Disconnect toolbar icon N
64. definitions 6 2 1 Audit trails 6 2 2 How back up and restore system definitions 6 2 3 Administration 6 6 2 1 System definitions General guidelines e System definitions are used to set up the systems in a UNICORN installation The definitions must be made for each new system that is installed e Ina network installation the definitions must be performed for each local computer in the network Note Access rights to a system are controlled at the user administration level see 6 3 User administration on page 137 Important The UNICORN computer name e The computer name in the UNICORN software must be the same as the Windows computer name The Windows computer name is therefore automatically filled in as the UNICORN computer name when you create a new system definition e Subsequently if the Windows computer name for some reason is changed after the UNICORN installation you manually have to change the computer name in UNICORN How to change the computer name The table below describes how to determine the Windows computer name and change the UNICORN computer name accordingly Step Action e Open Windows Control Panel and double click the System icon e Look for an item called Computer Name Note Depending on your Windows version you might have to click a tab to find the computer name e Take a note of the computer name and click the Cancel button Start UNICORN and choose Administration System Setu
65. der only to one or a few users In either case it is sufficient to grant access to C Unicorn Local Fil Failed since this is the path to the Failed folder on all local stations Access to all users If the system administrator grants access to the Failed folder to all users it will have the following implications e The individual user gets the responsibility to retrieve his or her own result files and to delete old files from the Failed folder e Any user will be able to examine copy move and delete the other users results in the Failed folder e The Failed folder can be used to temporarily store methods and results from runs performed from the local station when the network is not running Access to a few users If the system administrator grants access to the Failed folder to only one or a few users it will have the following implications e One or a few users will have the responsibility for retrieving the result files and deleting the old files from the Failed folder e The user s with access to the Failed folder should also have access to other users home folders to be able to copy or move result files to suitable destinations Note This policy should be used if the installation requires restricted access to users result files 03 0014 93 ep94 Security 5 5 3 2 Network communication failure local station In this section This section describes what happens when the network communication fails between a loc
66. described in 2 2 2 How to determine which CU is selected on page 28 Note The number of the selected CU must be entered in the UNICORN software installation so that UNICORN can find the system when started Installation 2 Step Action Locate an empty PCI slot e Install the expansion card in the empty PCI slot e Close the cover of the PC 6 Connect the card to the liquid handling module CU connector using the communication cable provided How to locate the The CU is set with a DIP switch Look at your expansion card and compare it with DIP switch the figure below The circle in the figure indicates where the DIP switch is located on the expansion card ep2 2 Installation 2 2 Hardware installation 2 2 2 How to install CU 900 PCI Howto determine The figure below describes which DIP switch setting corresponds to which selected which CU is selec Cy ted Dip switch setting Selected on the expansion Control unit card 2 2 Qj 2 N NJ N w N s 5 N Make a note of which CU is selected on the expansion card and save the note for future use When you install the UNICORN software and the setup program asks for the card settings type the number of the selected CU See also How to verify the CU setting of the expansion card How to verify the In the Controller Settings dialog box you can verify the CU Control Unit setting CU setting of the ofan expansion card ex
67. dialog box is displayed Choose Administration User Setup in the UNICORN Manager Select the user from the Users list and click the Edit button ep 161 6 Administration 6 3 User Administration 6 3 6 How to delete users and folders Step Action Select Access Folders in the left field of the User properties dialog box Select the user s folder in the Folders field if it is not automatically selected user properties x defaut defaut 4 Instructions New Delete LK coe Hee Click the Delete button and then the Yes button to confirm Note All methods result files and folders within a selected folder will be deleted when the selected folder is deleted Click OK to close the dialog box and then the Close button How to delete The folders described below must be deleted in a different way than described special folders above A home folder to which a user is assigned To delete a home folder to which a user is assigned e delete the user then the home folder See instructions above Or e change the home folder assignment for the user Select the User item in the User Properties dialog box and assign a different home folder A folder to which several users share access To delete a folder to which several users share access e first remove the access rights from each user deselect the folder for each user under Access Folders in the User properties dialog box then delet
68. e Follow the instructions in this section to install the program yourself if your system is not pre installed Note Ifthe system is connected to the network and installed to support remote control make sure that the same version of UNICORN is installed on all stations in the network In this section This section contains these topics Topic See How to install UNICORN 2 3 1 How to install UNICORN for AKTAxpress 23 2 How to install selected software components 2 3 3 e p35 2 Installation 2 3 Software Installation 2 3 1 How to install UNICORN 2 3 1 Different UNICORN ver sions Installation pre requisites 03 0014 93 ep 36 How to install UNICORN From UNICORN 5 01 on different versions of the UNICORN software are available Besides the Full version a Remote and a Dry version are also available The table below describes the characteristics of the different versions UNICORN version Characteristics Full version Complete functionality Remote version Only remote systems connected to an existing UNICORN server can be controlled Local systems cannot be installed Dry version The System Control module is not available There fore no systems can be controlled with this version of UNICORN In this chapter In this chapter the installation of the Full version of UNICORN is described The installation procedure for the other versions is similar though certain optio
69. e Click the Next button e Type a Password e Make sure that the Password Never Expires option is selected e Make sure that the User Must Change Password at Next Logon op tion is not selected Click the Next button and then the Finish button Double click the newly created user and select the Member of tab e Select the newly created group of UNICORN users and click the Add button e Click OK Click OK in the lt user gt Properties dialog box Repeat steps 5 11 to create more UNICORN users How to set up a shared folder an set the permissions Network setup 1 The table below describes e how to create a shared folder on the UNICORN file server e how to set the folder permissions so that access to the shared folder is only granted to users with full control in this case UNICORN users Step Action Log on with administrative rights to the UNICORN file server Open Windows Explorer Select the folder to use as a shared folder and choose File Properties e Select the Sharing tab e Click the Share this folder radio button and enter a suitable name e g UNICORN Click the Permissions button If the group Everyone already has the permission named Full Control then go to step 9 Click the Add button and make sure the correct domain is displayed in the top drop down menu e Select Everyone in the list box and click the Add button e Click OK Set the permission Full Control to the group Everyone
70. e Close button when the calibrations have been made Some KTAdesign systems have a pH monitor to allow online pH measurements Below is an illustration of the dialog box for pH calibration System Control 1 Explorer 100 Calibration Calibration procedure Monitor Calbvate pH electrode for bulter 1 See Help gt facoo pa 1 Reference value 1 0 0000 0 0 14 0 2 Read value 1 VEEE pH 3 Reference value 2 pooo 00140 4 I pH Calbvated electrode slope Asymmetry potential at pH7 mV Note Calibration can be performed with the electrode either within or outside the flow cell Instruction The table below describes how to calibrate a pH monitor with the electrode mounted in the flow cell Action e Attach the first pH reference solution for example pH 7 0 to Inlet A11 on the system e Attach the second reference solution for example pH 4 0 to Inlet B1 In the System Control module select Manual Pump Result The Instructions dialog box is opened Calibration 4 Step Action e Select Pump PumpWash Explorer in the Instructions field e Inthe parameters field select Inlet A11 e Click the Execute button Result The pump and inlet tubing will now be filled with the first reference solution Wait until the PumpWash is finished before you continue to the next step e In the Instructions field Select Pump Flow e In the FlowRate box insert the flow rate that you will use later duri
71. e aborts if you try to store resulting curves at the position of an original raw data curve 03 0014 93 p 184 Troubleshooting 8 8 5 Advice on operation AKTAdesign systems In this section This section describes the following AKTAdesign system scenarios e Connected to a system but no system contact e Flow scheme does not display properly Connected to a system but no sys Scenario Solution tem contact You are connected to a system but e Check that the chromatography have no system contact system is turned on Indications In the System Control e Check that all cable connections e the option Connection in the Run are intact data pane says Yes e If the above actions do not help e the option Instruments says Scan try to restart both the computer ning and the system e there is no contact with the system after a period of waiting Flow scheme does not display prop Scenario Solution l ot The flow scheme is not displayed Choose Settings Control Panel Dis properly play Settings in the Windows Start menu to check that you have selected 65536 colors ep 185 8 Troubleshooting 8 6 Advice on operation CU 950 8 6 Advice on operation CU 950 In this section This section describes the following CU 950 scenarios e Data recovery OFF at connection loss e End is reached during connection loss e CU 950 USB cable is unplugged e The PC hard drive cras
72. e current user Alarms and warnings can only be acknowledged from the control mode connection Messages are col or coded Method instruc tions and manual instructions over ride system set tings Settings must be enabled System settings 7 Alarms and warnings are displayed in the Logbook pane of the System Control module with different colors see the illustration below 0 00 min Manual Run 2002 04 09 07 38 23 Result c4 0 00 min Flow 0 00 ml min Manual 0 02 min Pump 0 error 121 Parameter was out ofr 0 10 minA900TempHighWarmn 10 C 10 0 37 minAg00Temp High Alarm 12 C 12 0 37 min Pause 2002 04 09 07 36 48 0 37 min Pump 0 error 121 Parameter was out ofr e Warning messages are displayed in orange text e Alarm messages are displayed in red text Limits for certain monitor signals can also be set locally in a method if allowed by the system strategy In such case the method setting method instruction overrides the system setting as long as the method is running Example This feature allows for instance the pH warning limit to be set to one value during process operation and another during system cleaning Note Manual instructions also override the system settings Alarms are not active unless the mode is set to Enabled Verify the alarm mode for each instruction line in the list ep 167 7 System settings 7 2 Alarms settings Hysteresis 03 0014 93 e
73. e instruction set The method instructions do not corres pond to the components you have chosen for your system Solution There are several actions that you can take Check that the method has been connected to the correct system in either of these ways inthe System Method Connection dialog box when you use the Copy from external dialog box in the Save As dialog box in Method Editor If the system is custom designed go to the Method Editor select the red instruction and either delete it or replace it with a corresponding instruction if available from the Instruction box Repeat this for all red instructions before saving the method Check your system components under Administration System Setup in the UNICORN Manager Troubleshooting 8 After Windows The scenario below applies to local systems only not remote systems logout and login you cannot get a l system connection Scenario Solution You have logged out of Windows and Restart the computer in order to ob then logged in again but you cannot tain a system connection in get a system connection in UNICORN UNICORN Reason If you shut down Windows with the command Start Shut down Close all programs and log in as a different user you will not be able to obtain a System Control connection in UNICORN the next time you or another user logs on This is because this shutdown procedure automatically shuts down a number of
74. e names of these files Note The missing files are usually winnt adm and com mon adm Copy the missing files from the CD to the folder where Poled it exe is located Result Now the System Policy Editor should start when you double click Poledit exe Windows 2000 The table below describes some necessary folder and file administration on a Folder administra Windows 2000 workstation tion Step Action Log on to the workstation as a user that will run UNICORN and whose access you want to restrict Note This user must have Windows administrator rights e Open Windows Explorer e Create a folder called Programs in the UNICORN folder The path will be C UNICORN Programs if C is the directory where the UNICORN application is installed 03 0014 93 ep 100 Security 5 Step Action Place shortcuts to the files listed below in the UNICORN Programs folder e UNICORN Bin UNICORN exe e The manuals that will be used located in UNICORN Manuals and UNICORN HtmlManual e Poledit exe and Poledit chm located in the folder that was created in Install the System Policy Editor above Note The Poledit exe and Poledit chm shortcuts should be removed when all the instructions in this section have been performed e Place ashortcut to UNICORN Bin UNICORN exe in the folder Documents and Settings lt user gt Start Menu Pro grams Startup Note lt user gt is the identity you used to log on
75. e table below describes two parameters in the Curves settings Parameter Time between samples Description Determines whether or not the curve data is stored in a result file in UNICORN Caution Check that Store is set to ON for all signals that are to be stored If a curve is set to OFF data from the monitor concerned cannot be displayed in the curves window during a process run and will not be recorded in any way Determines the frequency with which curve data is recorded in UNICORN this does not affect the reading frequency of the monitor itself The default setting is for the shortest possible time between samples e p 171 7 System settings 7 6 CU 950 settings 7 6 Introduction Only CU 950 Ad vanced settings can be changed Data recovery in struction 03 0014 93 e p 172 CU 950 settings This section describes the CU 950 settings which are available in the Instructions dialog box in the System Control module xi Instructions Data recovery Parameters C Sys0 Data recovery C Syst Toraen ki CONTINUE B on C Sys2 C Sys3 C Curves cu 350 Set Selected Parameter To Strategy Default Value Note How to open and use the Instructions dialog box is described in 7 1 The Instructions dialog box on page 164 CU 950 Advanced The CU 950 settings are only visible in the Instructions dialog box if the CU 950 Advanced controller is installed The CU 950 Advanced is c
76. e the folder as described above 03 0014 93 ep 162 System settings 7 7 System settings Introduction Each installed system has a set of default system settings which can be changed This chapter gives an overview of the system settings and describes how to change them In this chapter This chapter contains these sections Topic See Alarms settings The instructions dialog box Specials settings Monitors settings Curves settings CU 950 settings 7 6 e p 163 7 System settings 7 1 The Instructions dialog box 7 1 The Instructions dialog box Default system The system settings have default values which depend on the strategy used and settings which are valid for all of the runs If you assign a new value to a system setting the new value remains until you change the value again or return the setting to its strategy default value Instruction The table below describes the Instructions dialog box and how it is used to change the system settings Note To change the system settings you need to have System settings access see 6 3 User administration on page 137 Step Action Choose System Settings in the System Control module Result The Instructions dialog box for the connected system is dis played system Alarms Instructions i x Instructions Alam UVI Parameters C Alaims HighAlasm 25 0000 mAU C Disabled Ensbled C Specials LowAlarm 0 0000 mA Alam_Cond C
77. em Control window which can connect to all the systems in stalled Quick switching is possible with the new system navigator Click the Next button to continue Step 8 Select In the Select Program Folder dialog box you choose where to store the program Program Folder icon Select Program Folder x Setup will add program icons to the Program Folder listed below You may type a new folder name or select one from the existing Folders list Click Next to continue Program Folders Unicorn Existing Folders Administrative Tools Common Adobe Acrobat Intel LANDesk Virus Protect 5 0 Microsoft Developer Network Microsoft Visual C 5 0 e p61 2 Installation 2 3 Software Installation 2 3 2 How to install UNICORN for AKTAxpress The table below describes how to select a program folder for the UNICORN icon Step Action In the Select Program Folder dialog box you select the Start menu folder where you want the UNICORN icon to be placed You can either e accept the suggested folder named UNICORN recommended or e create a new folder Type the name of the new folder in the text field Program Folders or e select a folder that already exists by clicking its name on the list Click the Next button to continue Step 9 Start The Start Copying Files dialog box displays the installation choices made Copying Files Start Copying Files i x Setup has enough info
78. ent sounds can be assigned to each event Administration 6 How to print user The table below describes how to print the properties for selected users setup information Action Choose Administration User Setup in the UNICORN Manager Result The User Setup dialog box is displayed Select a user and click the Print button Result The Print dialog box is displayed e If desired click the Select All button to print information for all users e Select the check boxes for the Print Items that you want to include e Click OK e p157 6 Administration 6 3 User Administration 6 3 5 How to change user passwords and user attributes 6 3 5 Introduction Rules and recom mendations for UNICORN pass words How to change the password for a selected user 03 0014 93 ep 158 How to change user passwords and user attributes This section describes e how to change the password for a selected user e how users change their own passwords e how users change their user attributes Changes to user passwords and user attributes are made in the UNICORN Manager module The list below summarizes rules and recommendations for UNICORN passwords e The system can be set up to operate without required passwords The minimum number of password characters is set up at the installation Passwords can be any combination of letters and numbers Passwords are case sensitive Avoid using obvious pass
79. ep 173 8 Troubleshooting 8 Troubleshooting Introduction This chapter describes different operational scenarios which may arise in UNICORN and their solutions or consequences In this chapter This chapter contains these sections Topic See Advice on operation Logon Advice on operation UNICORN access Advice on operation Evaluation Advice on operation AKTAdesign systems Advice on operation CU 950 8 6 Advice on operation Methods 03 0014 93 ep174 Troubleshooting 8 8 1 Advice on operation Logon In this section This section describes e how to log on to UNICORN e the following logon scenarios Unable to log on to UNICORN Error message Strategy file error How to log on The table below describes how to log on to a system Step Action Start UNICORN e Choose your user name from the Username drop down list in the Logon dialog box e Type your password in the Password field e Click OK CE x A WY UNICORN logon User name default z Password ioana Cancel Help Note If you have forgotten your password ask the system ad ministrator for a new one e p 175 8 Troubleshooting 8 1 Advice on operation Logon Unable to log on to UNICORN Error message Strategy file er ror 03 0014 93 ep 176 Scenario Username and password not accepted You cannot log on although you use your correct username and password Po
80. ery important See 6 3 5 How to change user passwords and user attributes on page 158 Each user is assigned to an access group that defines e the operations that the user can perform e the folders the user is allowed to access Access groups are described in 6 3 4 How to assign user properties on page 149 When you install UNICORN a default user with full access rights is automatically created For system security reasons the default user must be deleted when you have created the site specific users To maintain system security only the system administrator should be allowed to carry out administrative routines such as user definition and system definition 9 2 Purpose Control mode and View mode con nections How to lock a system When to lock a system Security 5 Connection security The purpose of connection security is to avoid conflicts regarding system control so that only one user at a time controls the chromatography system Two kinds of connection modes can be established with the UNICORN system e Control mode connection The user has full system control that is control of the chromatography system e View mode connection The user can view the process but not control it To prevent conflicts the system can have only one control mode connection at a time that is only one user at a time can control the process To prevent other users from establishing a control mode connection t
81. es 54 Software license agreement 55 Strategy files 63 Template files 63 system 71 L Leave and lock MethodQueue run 79 Leave and lock scouting run 79 control mode conenction 79 Leave control dialog box 82 Leave system 79 Limits warnings and alarms 166 Local connection 76 drawbacks 76 Local station failure 96 transfer of the result file 97 auto save feature 97 Local station is not logged on save the results 97 Local workstation definition 4 Lock a system 91 rule 91 Lock system 79 03 0014 93 epvi Locked system 79 Locked system 81 Log off 82 M Manuals 72 Maximum resolution ensure 193 Menu commands system control 74 Monitor calibration 86 Monitors settings 170 Network administrator responibilities 4 Network communication failure 93 Network communication failure 95 Network environment who can perform the setup 7 Network installation 74 Network logon 76 Network recommendations 190 example 8 Network setup using TCP IP and Windows 2000 set up shared folder 11 set user rights 13 network components to install 13 install network components instructions 14 connect workstation to domain 14 map drive letter and install Unicorn 15 map shared folder instructions 15 install Unicorn instructions 16 set user rights 17 network components to install 18 install network components instructions 18 connect workstation to domain 19 map drive letter and install Unicorn 2
82. estarted Complete UNICORN Setup e Click the Finish button to exit the setup program and automatically restart the computer ep53 2 Installation 2 3 Software Installation 2 3 2 How to install UNICORN for AKTAxpress 2 3 2 How to install UNICORN for AKTAxpress Before you start Before you start the installation procedure the following prerequisite has to be met e The operating system Windows 2000 XP must be correctly installed on your computer See the operating system documentation for details Also notice the following e Youcan exit the installation at any point by clicking on either the Cancel button or the Exit button If you do this however the installation will be incomplete and the software cannot be used e After the installation the installed files are compared with the original files on the installation CD to make sure that no files have been corrupted during installation The criteria used for the file comparison are name size version and checksum Upgrading a Installing a new version of the UNICORN software over an existing UNICORN OE install installation is no problem You do not have to uninstall the previous version before installing the new version Note Ifyou have made additions to the previous installation you will be asked if you want to keep these additions when UNICORN is started the first time after the new installation This is described in 2 3 1 Step 4 Select Components on page 4
83. explanation below before the failure Results after this time will be lost Auto save feature An auto save feature saves a temporary result file on the local station every five minutes during runs see 6 2 1 System definitions on page 115 If a run is performed on a local station which is not logged on to the network the result file cannot be saved on a network drive Instead the results will be saved in the Failed folder on the local station e p 97 5 Security 5 4 How to prevent accidental shut down 0 4 How an accidental shut down could occur How to prevent a shut down 03 0014 93 e p98 How to prevent accidental shut down Chromatography systems may be controlled without running the user interface modules This is possible in the following cases e Network installation If a system is controlled from a remote station without starting UNICORN on the local station e Stand alone installation If a user quits UNICORN after starting a run In both these cases it is not apparent from the desktop that the UNICORN control software is actually running Therefore there is a risk that someone shuts down the computer in the belief that it is not in use Make sure you follow the recommendations below to prevent an accidental shut down of a control station which controls a UNICORN run Do not quit UNICORN if you are controlling a system e Do not turn off local station computers in a network installa
84. f the screen to the printer Scenario Solution You are unable to perform Quit or Lo You might be running a Scouting goff from UNICORN for a connection method or a MethodQueue These functions require a control mode con nection in order to start subsequent cycles correctly Action Stop the Scouting method or MethodQueue before you quit or log off Scenario Solution Monitor signals do not appear in the e Choose System Settings Curves in Curves pane in System Control System Control e Set the Store option to ON UV1 Parameters Store gsssssen IC OFF ON Signals for which Store is set to ON can be selected from the View Proper ties Curves dialog box in System Con trol Error message Couldn t create result file Destin ation path could not be found The Method Sys tem Connection dialog box keeps appearing The Method Edit or window does not fit on the screen Troubleshooting 8 Scenario If you receive the error message Couldn t create result file Destination path could not be found at the end of a method the local computer was un able to access the folder specified in the result file path Consequence This may happen if the specified folder is on the network server and network communication has been lost The result file is instead saved in the Failed folder on the local station Scenario If the Method System Con
85. fewer choices is used e Click the button that corresponds to the system you wish to install UNICORN for In the Select Components dialog box you choose the components to install by selecting the appropriate check boxes Select Components x Select the components you want to install clear the components you do not want to install Components Program Files User Information Strategy and Template files System Installation This component includes the UNICORN application files Space Required AHOK Space Available 1558180 K lt Back Next gt Cancel e p39 2 Installation 2 3 Software Installation 2 3 1 How to install UNICORN 03 0014 93 e p40 Note System Installation is not available in the Remote and Dry versions of UNICORN The table below describes each component and possible sub components Component Program Files User Information Description The Program Files check box must be selected whether it is a local or remote station Stand alone installation The User Information check box must be selected if you are installing UNICORN for the first time on a stand alone computer Network installation The User Information component only needs to be in stalled once in a network For all following installa tions to the same network the files will already be present on the server The Select Sub components dialog box Select User Information check b
86. following will happen e The run continues under the control of the local station e Results that were destined for network drives cannot be saved in their correct folders Instead the results are saved in the Failed folder on the local station Failed folder path If C is the UNICORN installation drive the path to the Failed folder is C Unicorn Local Fil Failed There the results can be retrieved when the run is completed Result file name The name of the result file will be the same as the original result file name Files with the same name base are distinguished by an incremental serial number in the same way as result files in any other folder Follow the instructions in the table below to retrieve the results from the Failed folder Step Action Start UNICORN unless already started on the local station connec ted to the system that was run Log on as an authorized user with access to the Failed folder e Reestablish the network communication e Move the result file from the Failed folder to a suitable location on the network server where it is accessible from remote stations ep 93 5 Security 5 3 Data security 5 3 1 Network communication failure remote station Policies for access The system administrator may choose one of the following policies regarding access tothe Failed to the Failed folder e To grant access to the Failed folder to all users e To grant access to the Failed fol
87. ginal desktop e p 109 5 Security 5 6 The Options dialog box Hee Evaluation The table below describes the commands of the Evaluation field ie Command name Function Show negative reten Select the check box to show negative retentions in tions the Evaluation module This means that curve data before the injection point will be displayed that is before time or volume is zero Deselect it to hide curve data before the injection point Asymmetry ratio at The value specifies the height in percent of peak height where the asymmetry calculation is performed See Appendix B3 of the UNICORN User Reference Manual for more information Resolution algorithm Select the resolution algorithm to be used See Ap pendix B3 of the UNICORN User Reference Manual for more information The Oligo field The table below describes the function of each checkbox when it is selected Note These functions are only valid for oligosynthesis systems Checkbox name Function Start message Inserts a start message in the method Sequence check Checks a sequence for errors Sequence paste Allows paste of sequence characters from another program The OPC field The table below describes the commands of the OPC field Command name Function Logon Logoff security Enables the OPC security interface All Users Displays all users in Data Access and Historical Data Access Take Control Automatically tries to gain
88. good idea to use a directory on a Windows server for easy backup 03 0014 93 ep6 1 2 Who can perform the network setup UNICORN ver sions Windows user right If named pipes cannot be used Network setup 1 Network environment The network setup should be performed by someone with experience in Windows 2000 XP and network installations Preferably a competent network administrator should be involved in the network setup the installation of the UNICORN software and the maintenance of the network Reference Network recommendations are listed in A 1 System recommendations on page 189 All computers in the UNICORN network must have the same version of the UNICORN software installed both the computers directly connected to chromatography systems and the remote control computers The user must have the Windows user right Access this computer from network to connect to the local station in remote control In some networks the policy is to not allow named pipes In such a case TCP IP communication via sockets must be used Follow the instructions in the table below to enable sockets and disable named pipes Action Choose Administration System Setup in the UNICORN Manager Result The System Setup dialog is displayed Click the Socket button in the System Setup dialog Result The Socket dialog is displayed e Check the check box in the Socket dialog Socket si x Note If t
89. gy Files check box Note Normally both strategy and template installation is selec ted e Click the Continue button The Start Copying Files dialog box is displayed e Click the Next button The Strategy and Template Installation dialog box is displayed e Select the instrument type in the Type list e Select the exact instrument in the Strategy template list e The Options field will reflect the choices made in step 2 above Install strategy and or Install template e Click the Install button Result Which dialog is displayed depends on whether strategy in stallation was selected or not 03 0014 93 ep 70 Installation 2 Step Action Strategy installation was selected typical The Enter Information dialog box is displayed e Accept the suggested name or type a new name for the strategy This will not affect any existing method or result files in the sys tem e Click the Next button Strategy installation was not selected rare The Select Strategy dialog box is displayed To install the template files select the correct strategy and click on the SELECT button To abort the template installation click on the CANCEL button Strategies installed on the system Cancel The dialog box shows all the strategies that are installed on the sys tem e Select the strategy to which the method template files correspond e Click the Select button Note Templates are installed on the ne
90. he logon pass word The Access item The Access item is used to define which folders and systems that the user has access to Step Action 1 e Open the User properties dialog box see the table in the beginning of this section and select the Access item Result The Folders and Systems subitems are displayed e Click the plus signs to expand the subitems see the illustration below User amp Attributes Advanced x Folders fl c Default c Failed on EH Systems v system Instructions 03 0014 93 ep154 Administration Step Action The Folders subitem The user has access to all files and sub folders in the selected folders Up to 20 folders can be set up Only folders that are selected will be visible in the Methods and Results windows of the UNICORN Manager module Note All users should have access to the Failed folder on each local station in a network installation This will ensure that users can access results that were saved in the Failed folder in case of a network communication error The Systems subitem The selected systems are available for the user This step describes how to create a new folder and give the user has access to it To create a new folder e select the Folders item e click the New button to display the Create New Folder dialog box x Drive Cc Folder name maoo Cancel Help e select the D
91. he user can take ation and possible actions Status bar text Possible actions nothing Not connected To establish a connec tion either e click the System Con nection icon or e select System Connect Controlled by lt user gt Control mode To leave the system but retain the connection with the System Control module either e click Disconnect from the System icon or e select System Discon nect Note You may leave the system locked or un locked Controlled by lt other View mode None Even if you click user gt The indicated user has a the System Connection icon it has no effect control mode connec tion e p77 3 System connections 3 3 Connection modes Status bar text Locked by lt other user gt System is available Connection mode View mode The indicated user has left the system in a locked state View mode A user has left the sys tem in an unlocked state Possible actions Click the System Connec tion icon to establish a control mode connec tion You must supply a password either e the locking password or e your logon password if you have Unlock locked systems ac cess Note The password is case sensitive Click the System Connec tion icon to establish a control mode connec tion 03 0014 93 ep 78 System connections 3 3 4 How to leave and lock a system Instruction Follow the steps in the table below to leave and
92. hes during a run Data Recovery OFF at connection Scenario Solution l iii The connection between the PC and To be able to maintain correct time CU 950 is temporarily lost duringa and volume after connection loss the method run When the connection is CU 950 Advanced must be used with reestablished incorrect time and Data Recovery set to ON volume is shown Note Either CU 950 USB is used or CU 950 Advanced with Data Recovery set to OFF End is reached during connection Scenario Consequence loss End of run is reached either The time stamp indicating the time of e during connection loss day not the method time will indicate the time when the message reached the or PC e during data upload after connec tion loss CU 950 USB The table below describes a CU 950 USB scenario cable is unplugged Scenario Solution A CU 950 USB is connected to the PC e Replug the cable into the PC and the USB cable gets unplugged for e Restart the PC some reason 03 0014 93 ep 186 Troubleshooting 8 The PC hard drive The table below describes a CU 950 Advanced scenario crashes during a run Scenario e The unlikely event that the hard drive crashes unrecoverably during a run e The CU 950 Advanced is used which stores the run data on its memory card Solution In order to retrieve the data from the CU 950 Advanced you need to e recover the backup files fr
93. hese settings are changed all computers in the UNICORN network have to be restarted Socket Port Base 2000 30000 i Cancel Help e Click OK e Click Close ep7 1 Network setup 1 3 UNICORN network example 1 3 Network illustra tion Comments to the illustration 03 0014 93 ep8 UNICORN network example The figure below illustrates how a UNICORN network can be organized PC with UNICORN Chromatography LJ system An PC with UNICORN Network sever Chromatography system Chromatography system Chromatography system PC with UNICORN PC where UNICORN PC with UNICORN is NOT installed Below are some comments to the network illustration shown in the figure above PCs 1 and 5 are local stations they have UNICORN installed and are directly connected to chromatography systems To have a chromatography system accessible remotely the local station must be switched on and logged on to the network PCs 2 and 3 are remote stations they have UNICORN installed but are not directly connected to chromatography systems Via the network the remote stations can control the chromatography systems that are connected to the local stations PC 4 does not have UNICORN installed and therefore cannot control any chromatography systems although it is connected to the network The network server does not have UNICORN installed and is not involved in the
94. his section have been performed e Place ashortcut to UNICORN Bin UNICORN exe in the folder Documents and Settings lt user gt Start Menu Pro grams Startup Note lt user gt is the identity you used to log on in step 1 e Right click the Windows Start button and select Properties e Click the Start Menu tab select the Start Menu radio button and click the Customize button e Click the Advanced tab in the Customize Start Menu dialog box e In the Start menu items field select the option Don t display this item for the following items Control Panel My Computer My Documents My Music My Pictures e Inthe same field uncheck the following items Run command Search e In the Recent documents field uncheck the box List my most re cently opened documents Log off and log on as the same user as in step 1 Security 5 Step Action 5 e Delete the folder Documents and Set tings lt user gt lt user gt s Documents Note lt user gt is the identity you used to log on in step 1 e Remove the shortcuts to Windows Explorer Command Prompt Tour Windows XP usually located in the folder Documents and Set tings lt user gt Start Menu Programs Accessories e Click OK and then OK again e Click Start Control Panel and double click Network Connections e Right click Local Area Connection and select Properties e Click the General tab and uncheck Show icon in notification area
95. ient for Microsoft Networks is already installed go to step 8 If not select the component Client from the list and click the Add button Select Client for Microsoft Networks from the list and click OK Click the Install button If the component File and Printer Sharing for Microsoft Networks is already installed go to step 11 Otherwise select the component Server from the list and click the Add button Select File and Printer Sharing for Microsoft Networks from the list and click the Add button Click the Install button N If the component Internet Protocol TCP IP is already installed go to step 14 If not select the component Protocol from the list and click the Add button w Select Internet Protocol TCP IP and click OK A Select Internet Protocol TCP IP and click Properties n Configure the TCP IP protocol with network specific information How to connect Follow the instructions in the table below to add the workstation to the Windows the workstation to gomain the domain i Step Action Log on to the Windows 2000 workstation with administrator rights 2 Select Start Settings Control Panel 03 0014 93 ep14 To map a drive letter and install UNICORN How to map the shared folder Network setup 1 Step 0 11 12 3 Action Double click the System icon Select the Network Identification tab and click the Network ID button Result The Net
96. in step 1 e Remove the contents in the folder Documents and Set tings lt user gt Recent e Log off and log on as the same user as in step 1 e Delete the folder Documents and Settings lt user gt My Documents Note lt user gt is the identity you used to log on in step 1 e Remove the shortcut to Windows Explorer located in the folder Documents and Settings lt user gt Start Menu Pro grams Accessories e Log off Windows XP The table below describes some necessary folder and file administration on a yee administra Windows XP workstation Step Action Log on to the workstation as a user that will run UNICORN and whose access you want to restrict Note This user must have Windows administrator rights e Open Windows Explorer e Create a folder called Programs in the UNICORN folder The path will be C UNICORN Programs if C is the directory where the UNICORN application is installed ep 101 5 Security 5 5 Single application mode 03 0014 93 ep 102 Step Action Place shortcuts to the files listed below in the UNICORN Programs folder e UNICORN Bin UNICORN exe e The manuals that will be used located in UNICORN Manuals and UNICORN HtmlManual e Poledit exe and Poledit chm located in the folder that was created in Install the System Policy Editor above Note The Poledit exe and Poledit chm shortcuts should be removed when all the instructions in t
97. in the UNICORN Manager Result The System Setup dialog box is displayed e p117 6 Administration 6 2 System administration 6 2 1 System definitions Step Action 2 Select the system in the System Setup dialog box and click the Edit button Result The Edit System dialog box is displayed Edit System ChromatographylAKTA M E100F400 E 03 0014 93 e p118 Administration 6 Step Action 3 The fields of the dialog box are described below Note Any other changes than Strategy Computer name and Component require that you run the UNICORN Setup CD See 2 3 3 How to install selected software compon ents on page 67 e System name Type the system name Names can be up to 30 characters long Note The system name can be set only when a new connec tion is defined It cannot be edited later since user ac cess rights are linked directly to the system name e System type Select a system type either Chromatography Oligo or Xpress e Strategy Select a strategy for the system from the drop down list Click the Information button to display information about the selected strategy The Component button is described in step 4 below Note Available strategies are determined when UNICORN is installed see 2 3 Software installa tion on page 35 If there are several strategies in stalled make sure that the selected strategy is appro priate for the system Step continued belo
98. ing installation will not be erased You can regain access to these files by re defining users with appropriate folder access Note All the components in the dialog box are installed on the network server in a network installation The table below describes how to install Strategy and Template files Note The instructions below are more detailed than the other instructions in this section This is because the Select Strategy dialog box is not described anywhere else in this manual Step Action 1 e Select the Strategy and Template files check box in the Select Components dialog box e Click the Change button Result The Select Sub components dialog box is displayed ep 69 2 Installation 2 3 Software Installation 2 3 3 How to install selected software components Step Action Select Sub components xj Select the components you want to install clear the components you do not want to install Sub components Strategy files Template files OK r Description This component includes all files describing a chromatography system l Space Required 49540 K Space Available 405120K Select the Strategy files check box if you want e to re install system strategies and wizard e to install additional strategies Select the Template files check box if you want e to re install template files e to install template files for a new strategy Then you must also select the Strate
99. ings cu 950 USB Control Unit fi CU 950 Advanced CU 900 PCI Help Default Clear Cancel e In the Controller field select the CU 900 PCI option e Type a new System name if you want to change the suggested name e Choose the System type and Strategy e Set the Control Unit number 1 4 e The Default button sets the default resource settings for the selected system type e The Clear button clears all fields if you want to remove a system e When you are finished click OK The System Installation screen is displayed again showing the options you have chosen Note If you installed more than one system make a note of which system is connected to which control unit This in formation will be useful when you set up the system table or if you must reinstall UNICORN in the future e Click the Next button to copy the necessary files If there is more than one system to define repeat steps 1 to 4 in this table for each system When all systems that are connected to the station have been defined click the Next button in the System Installation dialog box to continue Installation 2 Note If you want to define systems later or change the settings for a previously defined system run the setup program once again with only the System Installation option selected in the Select Components dialog box Step 12 Setup The installation is complete and the computer must be r
100. ion between the PC and the chromatography sys tem e Check that the power to the chro matography system is turned on e Switch off the chromatography system e Quit UNICORN e Shut down and restart the com puter Check that you have access rights to the system Access rights are not automatically assigned for a newly defined system e p177 8 Troubleshooting 8 2 Advice on operation UNICORN access 03 0014 93 ep 178 Scenario You receive the error message Can not connect to system in a network installation No contact between the System Control and the system Possible reason Sometimes this can be due to the fact that named pipes cannot be used Then you have to en able sockets instead of named pipes Socket communication fails and the OCI crashes at start up Possible reason The port number as signed in the Socket dialog box is used by another application Solution e Check that the local computer to which the system is connected is turned on and logged on to the network e Check that the computer from which you try to establish a connec tion is logged on to the network e Check that the limit of 8 connec tions to the system has not been exceeded e In the UNICORN Manager choose Administration System Setup to open the System Setup dialog e Click the Socket button and check the check box in the Socket dialog e Click OK then the Close button
101. is displayed 03 0014 93 ep18 Network setup 1 If the component Client for Microsoft Networks is already installed go to step 5 Otherwise e Click the Install button select the component Client from the list and click the Add button e Select Client for Microsoft Networks from the list and click OK If the component File and Printer Sharing for Microsoft Networks is already installed go to step 6 Otherwise e Click the Install button select the component Service from the list and click the Add button e Select File and Printer Sharing for Microsoft Networks from the list and click OK If the component Internet Protocol TCP IP is already installed go to step 7 Otherwise e Click the Install button select the component Protocol from the list and click the Add button e Select Internet Protocol TCP IP from the list and click OK e In the Local Area Connection Properties dialog box select Internet Protocol TCPAP and click Properties e Configure the TCP IP protocol with network specific information How to connect Follow the instructions in the table below to add the workstation to the Windows the workstation to gomain the domain Step Action Log on to the Windows XP workstation with administrator rights Choose Start Control Panel System Result The System Properties dialog is displayed Choose the Computer Name tab and click the Change button Result The
102. k boxes for the items you want to display Note All items are recorded in the audit trail The check boxes only control which items are displayed in the dialog box ep 125 6 Administration 6 2 System administration 6 2 2 Audit trails The Global tab The illustration below shows the Global tab of the Audit Trail dialog box DA Audit Trail Bei Es Fie Edit View Help Global TheSystem BE Oligo system BE MtpTest BF Munster BF Pa Hal gt The following files are used in this summary I User 2001090300 Monday September 03 2001 2001081000 Monday Septenbe 19 a IV Backup 1091 Monday September 17 2001 2001092400 Monday September 24 2001 M Logon Logott 2001100100 Monday October 01 2001 I Define system 2001100800 Monday October 08 2001 PE ia d diti I7 MethodQueue 2001 09 03 021222 Logon Kael 7 2001 09 03 03 14 02 Logoft Kael M Maintenance 2001 09 03 03 15 04 Logon Kael I Information IT Timezone WT NONE 1 ALF I anali V mat The items of the Global tab The table below describes the items which can be displayed on the Global tab Item Displays e user creation deletion and redefinition e creation of rescue file for system definitions e restoration of system definitions with rescue file e changes of global and personal lists column list report format templates evaluation procedures e column protection mode enable disable backup operation
103. lates gt Windows Components gt Windows Explorer In the right window pane double click the setting Prevent access to drives in My Computer Click the Policy tab Click the Enable radio button Click the Apply button and then OK Log on to the computer as an administrator Verify that access to all the drives is restricted apply to all users including administrators See 5 5 Windows 2000 Exclude administrator from policy changes on page 107 on how to exclude real administrators from being affected by the group policy changes Log off from the computer Log on to the computer as a user whose policies you want to re strict Verify that the restrictions are in place for example that the user is unable to access to any drives e p 105 5 Security 5 5 Single application mode Windows 2000 Exclude adminis trator from policy changes 03 0014 93 ep 106 When you have restricted the access in Windows Explorer as described above these restrictions will apply even to the real administrator of the computer Therefore you must exclude the real administrator from those policy changes Note You do not have to perform this in Windows XP The table below describes how to do this Step Action Log on to the computer as a real administrator who you want to exclude from being affected by the policy changes performed above Copy the file C WINNT System32 GroupPolicy lt user gt Re gistry pol
104. le for network setup software installation and software maintenance e UNICORN administrator or system administrator Responsible for the use of UNICORN to control chromatography systems The network administrator and the UNICORN administrator can be the same person but the tasks can also be carried out by two different persons Administrator se The different security responsibilities of the network administrator and the we responsibil UNICORN system administrator are listed below Area Network administrator UNICORN administrat responsibilities or responsibilities Data storage security Back up routines for Control user access to back up routines server and local disks home and shared folders for example to place all home folders on a shared disk to pre vent data from being scattered through the network Network access security Maintenance of user passwords and access rights to shared re sources UNICORN security Maintenance of user profiles Local and remote In a UNICORN network the workstations can be categorized as either local stations stations or remote stations Local station A PC to which a chromatography system is physically connected 03 0014 93 ep4 Network setup 1 Workstation type Description A PC to which no chromatography system is physically connected but which can control systems over a net work link On a remote station the UNICORN software is inst
105. lect the Attributes item in the dialog box See 6 3 4 The Attributes item on page 153 for an explanation of the Attributes item e Make the desired changes Click OK and then the Close button How users change The table below describes how users change their own logon and signature their own pass words passwords Action Choose Administration Change Password in the UNICORN Manager Result The Change Password dialog box opens Change Password e default m Logon password New Confirm e Type the logon password in the Old text box of the Logon password field Note The passwords will only be shown as asterisks 1 m Signature password Old premem Old XXXXXXXKKH New xxxxx Cancel Help Type a new password in the New text box Repeat the new password exactly in the Confirm text box x e p 159 6 Administration 6 3 User Administration 6 3 5 How to change user passwords and user attributes Step Action To define a Signature password repeat step 2 in the Signature pass word field Note The signature password cannot be the same as the logon password Click OK How users change The table below describes how users can change their own user attributes their own user at tributes Step Action Choose Administration Change User Attributes Result The Change user attributes dialog box opens Change User Attributes x
106. licit authorization to use the Calibrate commands in System Control to use the Tune commands in System Control to change system settings with the Settings command in System Control Note Any user can view the system settings but this access item is required to make changes to the settings to use the MethodQueue editor to run MethodQueues ep 143 6 Administration 6 3 User Administration 6 3 2 Access items Access item Allows the user Edit global list s e to save amethod as a method template an evaluation procedure as globally available areport format as globally available acolumn in the Column list or BufferPrep recipe also Quantitation tables and Mol Size tables if the Analysis module is installed as globally available e to delete method templates global procedures global report formats global columns global BufferPrep recipes also global Quantita tion tables and global Mol Size tables e to restore global user information files user setup and system setup Note We recommend that this access item is re stricted to only one user in an installation Maintenance to gain access to the System Maintenance command in System Control Create Curve to create curves in the Evaluation module Rename Curve to rename curves in the Evaluation module Quit program to end a UNICORN session with the File Quit Program command in the UNICORN Manager 03 0014 93 ep144
107. llustration below displays the User properties dialog box with the Attributes item selected User properties J xj C Dave David Wilson Advanced 3 On Access 4 5 Instructions Attributes The table below describes the User Attributes User Attributes I Use large toolbar icons T Show unused variables I Show variable details I Default overwrite of baselines and peak tables T Prompt for column before manual runs Quick viewcuve fl Fraction mark height 2 Character heights gt Infection mark height 50 Percent of window height x Logbook mark height 3 Character heights z User Attribute Use large toolbar icon Show unused variables Show variable details Default overwrite of baselines and peak tables Prompt for column before manual runs Description Display large toolbar icons in all modules Show variables that are not used in the method on the Variable page of the Start Protocol Show detailed method variables on the Variable page of the Start Protocol When new baselines and peak tables are created the old ones are overwrit ten A manual run must have an Alarm_Pressure text instruction inser ted before the first instructions are executed Administration 6 User Attribute Quick view curve Fraction mark height Injection mark height Description usually the UV curve mark in a chromatogram mark in a chromatogram Select which of
108. lock a running system or an End state system which is connected with a control mode connection Step Action e Click the Leave control of system icon S OT e Select System Leave Control Result The Leave control of system dialog box is displayed Leave Control of system x Leave system Unlocked C Locked User name default Password Cancel Help e Select how you want to leave the system The Unlocked option leaves the system unlocked Any other user may establish a control mode connection to the system Use this option if you do not intend to use the system in the near future The Locked option locks the system with the password specified in the dialog box A control mode connection can only be es tablished by providing the correct password This password is independent of the user s logon password A locked system can also be unlocked with the logon password for a user with Unlock locked systems access This access item should be re stricted to a small number of users to prevent indiscriminate unlocking of locked systems e Click OK e p79 3 System connections 3 4 How to leave and lock a system Leave and lock a Scouting run or MethodQueue run If you leave and lock a Scouting run or a MethodQueue run it is not possible to establish a control mode connection from another computer 03 0014 93 ep 80 3 5 Instruction System connections 3 How t
109. ly on the target system Check Demo system if no systems will be physically connected to the computer r Setup Type C Network installation F Remote only system Server disk Stand alone installation I Demo system lt Back Cancel The table below describes the choices in the Setup Type dialog box Step Action Select either of the options e Network installation described in step 1 a below or e Stand alone installation described in step 1 b below la Network installation A network installation can be either e a local station e aremote only system You have to select these disk drives in a network installation e A local disk for the program files e A server disk for the server files Select the Remote only system check box to install a remote only system a computer to which no systems are physically connected Note When you perform a network installation the necessary UNICORN software components will be copied automat ically to the network server disk ep59 2 Installation 2 3 Software Installation 2 3 2 How to install UNICORN for AKTAxpress Stand alone installation A stand alone installation can be either e a local station e a demo station Select the Demo system check box if you want to install a demo sta tion The network options settings are ignored for a stand alone installa tion Note If you perform a stand alone install
110. manage system connections How to calibrate system monitors 2 5 03 0014 93 ep 22 Installation 2 2 1 Installation overview Installation sam The table below is an overview of the complete UNICORN installation procedure mary Step Action Back up files if you migrate from an older version of UNICORN to a newer version Set up the network environment for network installations only and the workstations Reference See 1 4 How to configure the network server on page 9 and 1 5 How to configure the workstations on page 12 Install UNICORN hardware and software Reference See 2 2 Hardware installation on page 24 and 2 3 Soft ware installation on page 35 Define access levels for the installation Reference See 6 3 1 User access groups on page 138 Define users with home folders and access profiles Reference See 6 3 3 How to create a new user on page 145 and 6 3 4 How to assign user properties on page 149 Check the system settings for the attached systems Reference See chapter 7 System settings on page 163 System recom The hardware software and network recommendations are listed in A 1 System mendations recommendations on page 189 ep 23 2 Installation 2 2 Hardware installation 2 2 Hardware installation Introduction The hardware can be of two types e Internal PCI expansion card CU 900 PCI e External controller CU 950 First of all read 2 2 1 When to install ha
111. manual chromatography software UNICORN 5 0 Administration and Technical Manual Amersham e Biosciences Gr 03 0014 93 UNICORN 5 0 Administration and Technical Manual 03 0014 93 Edition AC 2004 03 Office addresses Amersham Biosciences AB SE 751 84 Uppsala Sweden Amersham Biosciences UK Limited Amersham Place Little Chalfont Buckinghamshire England HP7 9NA Amersham Biosciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 USA Amersham Biosciences Europe GmbH Munzinger Strasse 9 D 79111 Freiburg Germany Amersham Biosciences K K Sanken Building 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Amersham Biosciences China Limited 13 F Tower I Ever Gain Plaza 88 Container Port Road Kwai Chung New Territories Hong Kong www amershambiosciences com Trademarks UNICORN AKTAxpress AKTAprime Ettan BioProcess Drop Design and AKTA are trademarks of Amersham Biosciences Limited Amersham and Amersham Biosciences are trademarks of Amersham ple Microsoft Windows and Windows NT are trademarks of the Microsoft Corporation in the United States and or other countries Terms and Condition of Sale Unless otherwise agreed in writing all goods and services are sold subject to the terms and conditions of the company within the Amersham Biosciences group which supplies them A copy of these terms and conditions is available on request Any use of this software is s
112. metry potential at pH 7 fields Note If you calibrate with the electrode outside the flow cell do not remove the electrode from Solution 1 until the Read Value 2 button has become available when the button text has turned from grey to black 03 0014 93 e p88 Security 5 5 Security Introduction This chapter presents the security concepts and features of a UNICORN installation The concepts and features are good to know since they are also used in other parts of this manual In this chapter The table below describes the contents of this chapter Topic See Access security Connection security Data security How to prevent accidental shut down Single application mode The Options dialog box 5 6 e p89 5 Security 5 1 Access security 5 1 Purpose Passwords to re strict access Access groups Delete default user Maintain system security 03 0014 93 e p 90 Access security The purpose of access security is to avoid unauthorized user access to the UNICORN system User access is often restricted by means of a password There are some password rules that are important keep in mind e The password must have a minimum number of characters The minimum length is defined when UNICORN is installed see 2 3 1 How to install UNICORN on page 36 and 2 3 2 How to install UNICORN for AKT Axpress on page 54 e The password should be changed regularly if access security is v
113. ministration 6 2 System administration 6 2 3 How to back up and restore system definitions 6 2 3 Introduction How to back up the system defini tions 03 0014 93 ep 134 How to back up and restore system definitions You can create a backup file with system information and store it on a diskette or another drive The backup file will contain information about e Global files global BufferPrep recipes global columns global evaluation procedures global report formats e Personal files personal BufferPrep recipes personal columns personal evaluation procedures personal report formats e System files user setup system setup Afterwards you can use the backup file to restore the system definitions if they are corrupted The table below describes how to create a backup file and store it for example on a rescue diskette Step Action 1 Insert a diskette into the computer if you want to store the backup file on a diskette Administration 6 Step Action Choose Administration Create Restore Backup in the UNICORN Manager to display the Create Restore Backup dialog box Create Restore Backup xj Action Create Restore Browse C UNICORN Global Files Global BufferPrep Recipes Global Columns Global Evaluation Procedures Global Report Formats Personal Files S System Files User Setup System Setup Information Cancel Help
114. n e Click the Exit button Note The reset time is recorded in the audit trail Other sources of information Administration 6 The table below describes how to open the Documentation dialog box Action e Open the Evaluation module or the System Control module e Click the View Documentation icon Result The Documentation dialog box is opened Select the Event Log or the Logbook tab to view some of the audit trail information See the example below Frac950 Variables Scouting Test Method Method Information Resutiniomaton Start Pr ii i i End block 2 25 06 ml Cornus 121314 Marua Sytem 28 68 ml AutoZor x 28 68 el End 28 68 mi Block Flowthrough_ Fractionation 28 68 ol SameasMan Bate Cts fave 22 84 ml End block 26 84 ml Block Autotarreler_rfection C Vamo 28 84 mi Base SameAsMan 2884 mi Sa Mak p Adu tetenion zero pm 28 86 ml Quaniiate Info Iiecton Vokme 0 400 mi Rope TE 28 86 mi Hold 1217 02 28 86 ml InjectionPorial 16 400 u Yes Noir 23 62 mi Sampleingect 23 62 ml Continue 1217 47 23 62 mi QuantiationD ata S 29 62 al 23 62 ml Block Wash_Out_Unbound_Sarple 23 62 ml Base SemetcMan 23 64 mi Quanttate Into Type 0 37 48 mi End block T Find Help Parts of the audit trail information can also be found in the Documentation dialog box available in the Evaluation and System Control modules ep 133 6 Ad
115. n V System settings V Edit MethodQueue V Run MethodQueve Edit dobal fist s Maintenance Create Curve Rename Curve V Quit program Cancel He Select a group in the left field to view its access items in the right field Access Note The authorization items that are valid for the group are written in bold style and have selected check boxes How to edit the UNICORN has 10 different access groups that can be named and assigned to ACCESS BTOUPS different levels of access to UNICORN The table below describes how to edit the access groups Action Choose Administration User Setup in the UNICORN Manager Result The User Setup dialog box is displayed Click the Access groups button Result The User properties dialog box is displayed Change the name of a group e Select the group in the left field of the dialog box e Type anew name in the Group text box e Click OK 03 0014 93 ep 140 Administration 6 Step Action 3 Choose access items for a group e Select the group in the left field of the dialog box e Select the check boxes in the right field Access to choose access items e Click OK Note If you edit the definition of an access group to which users are already assigned the changes will apply to all users in the group Special conditions Certain access items cannot be completely removed e User setup Groups At least one access group must have this access
116. n system monitor 86 Calibrate monitor pH monitor 86 Calibrate tune 84 Calibration continuous 85 Calibration facts one point calibration 84 two point calibration 85 Calibration methods 84 Calibration of monitors calibration facts 84 Calibration of monitors 86 Change password for a selected user 158 03 0014 93 epii Change password for a selected user 159 Change user attributes 158 Change user passwords 158 Close MethowQueue run 79 Close scouting run 79 Close system 79 Column files 68 Component Selection 49 Components installation 67 select 67 Connect system 75 Connect System Control module 75 Connection remote 75 local 76 modes 77 Connection management 74 Connection modes 77 Connection modes 91 Connection security purpose 91 lock a system 91 Connection Control mode 91 Connection View mode 91 Control capacity 191 network installations 191 Control mode connection 77 Create a new user 145 Create a new user instruction 146 Create new user home folders 148 Create New User dialog box 147 CU 900 PCI system setup 49 CU 900 PCI expansion card hardware installation 26 locate DIP switch 27 Index e piii Index determine which CU is selected 28 CU 950 hardware installation 30 system setup 49 advice on operation 186 CU 950 settings 172 Curves settings 171 parameters 171 Data buffers frequency 192 Data sampling storage 192 frequency 192
117. nection dialog box keeps appearing you have one or more methods that are not connected to a system Reason Most likely you have impor ted one or more methods with the command File Copy from External in the UNICORN Manager Solution Connect the method s to the appropri ate system Scenario The Method Editor window does not fit the screen and has scroll bars Reason The incorrect font size might be installed Solution e You need to install Small fonts in stead of Large fonts This requires that you have the Windows CD ROM that was shipped with your Compaq computer e Insert the CD ROM and follow the directions on the screen ep 181 8 Troubleshooting 8 3 Advice on operation Methods There are red in structions in a method 03 0014 93 ep 182 Scenario Red instructions instructions with a red dot in a method are syntax errors and may be due to the following e The method was connected to the wrong system that is the strategy of the system is incompatible with the method The Copy function was used instead of Copy from external when a meth od was imported from a disk The wrong system may have been selected in the Save As dialog box in the Method Editor You may also have templates not intended for your system which might be the case for custom de signed systems The systems strategy has been up dated with a new strategy that dif fers in th
118. network is assigned this access item Audit trail System setup to examine the audit trail and define connected sys tems Caution It is recommended that only one user in an installation or network is assigned this access item Delete move Home to delete and move files and folders within the user s only home folder It does not authorize these operations on other folders Delete move to delete and move files and folders both outside and within the user s home folder Copy file s to copy files The user must have access to both the source and target folders in order to move or copy between folders 03 0014 93 ep 142 Administration 6 Access item Confirm Sign Unlock locked system Run methods Manual interaction Calibrate Tune System settings Edit MethodQueue Run MethodQueue Allows the user e to confirm answers to start protocol questions to sign methods and result files to unlock locked systems with the user s own logon passwords Locked systems can normally only be unlocked with the locking password Note We recommend that this access item is re stricted to a few users in an installation The user who locks a system does not re quire this access item to unlock the same system to start methods to issue manual commands in System Control to pause a running process with the Pause button in System Control Note The Pause instruction in methods does not require exp
119. ng your run e Click the Execute button Allow at least 35 ml of reference buffer to pass through the cell during which time the pH should stabilize e Select System Calibrate from the System Control menu e In the Monitor drop down box select pH Note The Measured value field shows the actual reading accord ing to the previous calibration This value may be incorrect and does not affect the current calibration When the pH is stable do the following e Enter the known pH of the reference solution in the field Refer ence value 1 e Click the Read Value 1 button Switch to the second reference solution this way e Select Manual Pump e Select the instruction Pump Gradient e In the Parameters field set target to 100 B after O minutes e Click the Execute button e Select the instruction Pump PumpWashExplorer e Inthe Parameters field set Inlet B1 to ON e Click the Execute button Result Do not continue to the next step until the PumpWash is fin ished ep 87 4 Calibration 4 2 How to calibrate monitors for AKTAdesign systems Step Action When the pH is stable do the following e Inthe Calibration dialog box enter the known pH of the reference solution in the field Reference value 2 e Click the Read Value 2 button e Click the Close button e Click the End button to stop the flow rate After the calibration the values are automatically entered into the Calibrated electrode slope and Asym
120. ns are disabled Before you start the installation procedure the following prerequisites have to be met e The operating system Windows 2000 XP must be correctly installed on your computer See the operating system documentation for details e For network installations of UNICORN the network must be correctly set up See chapter 1 Network setup on page 3 Installation notes Upgrading a UNICORN install ation Do not copy the CD ROM or de compress the files Step 1 Insert the Setup CD Installation 2 Also notice the following e Perform the UNICORN installation procedure on each computer in the network for a network installation e A warning message is issued if you install UNICORN to an existing UNICORN server It is not possible for the Setup program to check which UNICORN version is installed on the server so the warning is general Warning You are installing UNICORN to an existing UNICORN server on the network All computers connected to the same UNICORN server on the network have to be of the same version for the software to work correctly Please check that all computers connected to the UNICORN server you are connecting to is of the correct version If another version is detected on one of the other computers connected to the UNICORN server those computers have to be upgraded to the same version you are currently installing e Youcan exit the installation at any point by clicking on either
121. ns to the Program Folder listed below You may type a new folder name or select one from the existing Folders list Click Next to continue Program Folders U nic ormi Existing Folders Administrative Tools Common Adobe Acrobat Intel LANDesk Virus Protect 5 0 Microsoft Developer Network Microsoft Visual C 5 0 The table below describes how to select a program folder for the UNICORN icon Step Action In the Select Program Folder dialog box you select the Start menu folder where you want the UNICORN icon to be placed You can either e accept the suggested folder named UNICORN recommended or create a new folder Type the name of the new folder in the text field Program Folders or e select a folder that already exists by clicking its name on the list Click the Next button to continue e p45 2 Installation 2 3 Software Installation 2 3 1 How to install UNICORN Step 9 Start The Start Copying Files dialog box displays the installation choices made Copying Files Start Copying Files x Setup has enough information to start copying the program files If you want to review or change any settings click Back If you are satisfied with the settings click Next to begin copying files Current Settings UNICORN 5 07 Software Setup Full Version User Information User name John Smith Company Amersham Biosciences System Type 1 KTA System except AKTAxpress
122. o convert monitor signals to appropriate units for display For monitors supplied by manufacturers other than Amersham Biosciences and for some Amersham Biosciences monitors calibration in UNICORN should be performed each time the monitor itself is calibrated For most monitors supplied by Amersham Biosciences calibration from UNICORN also performs a true calibration of the monitor adjusting the signal response level in the monitor Calibration access Monitors can only be calibrated by users with Calibrate Tune access Calibrations made directly on system modules are logged only in the System Control logbook and not in the Calibration page of Documentation dialog box Evaluation module or in the Audit trail Problem This means that the Calibration page and Audit trail information might not be up to date Solution Therefore always calibrate modules using the UNICORN calibration functions in the System Control module when possible Different calibration methods are used depending on the type of monitor and system e one point measurement d two point measurement e continuous measurement Note The calibration method depends on the routines established in the laboratory or process department One point calibration is based on measurements taken at one reference point The monitor will be calibrated based on an assumed linear response between the reference point and zero Calibration 4 Two point calibra Two
123. o disconnect a system Follow the steps in the table to disconnect a chromatography system from a System Control module Step Action e Click the Disconnect from system icon PL or e select System Disconnect Result e If you disconnected from a view mode connection you are now disconnected e If you disconnected from a control mode connection the Leave control of system dialog box is displayed Proceed to step 2 below Note You can disconnect a system during a run and the run will still continue It is not recommended to do this without locking the system since this can leave a run on the system with no responsible user You cannot however disconnect from Scouting or MethodQueue runs e p81 3 System connections 3 5 How to disconnect a system Step Action 2 This step is only relevant if you disconnect from a control mode connection Leave Control of system x Leave system Unlocked C Locked User name default Password Cancel Help e Select how you want to leave the system The Unlocked option leaves the system unlocked Any other user may establish a control mode connection to the system Use this option if you do not intend to use the system in the near future The Locked option locks the system with the password specified in the dialog box A control mode connection can only be es tablished by providing the correct password This password i
124. o the system a user should e establish a view mode connection to the system e lock the system with a password This password can be different from the user s logon password Note If a user leaves the system unlocked in a view mode connection any other user may establish a control mode connection to the system It is recommended that systems are always locked when a user leaves the system When the system is controlled and locked the responsible user is identified in the System Control window for view mode connections A system which is left unlocked with no control mode connection has no identified responsible user Systems may be locked even when they are idle to allow users to reserve a system for later use e p91 5 Security 5 3 Data security 9 3 Introduction In this section 03 0014 93 ep92 Data security Data security prevents that results of a run are lost due to failure in the network communication or the local station The table below describes the contents of this section Topic See Network communication failure 5 3 1 Local station failure 5 3 2 9 3 1 If the network communication fails Failed folder and result file How to retrieve the results from the Failed folder Security 5 Network communication failure remote station If the network communication fails while a method is running the remote station will lose control of the system Then the
125. ode should apply to You need to carry out several of the instructions in this section to configure single application mode on the user level Which instructions to use depends on the operating system of the workstation Windows 2000 workstations You must carry out the following instructions on a Windows 2000 workstation e Install the System Policy Editor e Windows 2000 Folder administration e Edit the system policy e Windows 2000 Restrict access in Windows Explorer e Windows 2000 Exclude administrator from policy changes Windows XP workstations You must carry out the following instructions on a Windows XP workstation e Install the System Policy Editor e Windows XP Folder administration e Edit the system policy e p99 5 Security 5 5 Single application mode Install the System The table below describes how install the Windows System Policy Editor on a Policy Editor Windows workstation Action Insert the installation CD supplied with Windows 2000 Server con taining the Policy Editor files e Open Windows Explorer e Create a folder in the root directory of the installation drive directly under C if C is the installation drive The name of the folder is not important e Copy the files Poledit exe and Poledit chm from the CD to the folder you created Open the folder and double click Poledit exe Result A message is probably displayed saying that files are missing Write down th
126. om the damaged hard drive if it is possible and move them to another PC e move the CU 950 to the other PC and restart the PC ep 187 A Technical specifications A Technical specifications Introduction This appendix describes e the UNICORN system recommendations e UNICORN s capability to control chromatography systems e how UNICORN samples data from the chromatography systems In this appendix The table below describes the contents of this appendix Topic See System recommendations UNICORN control capacity Data sampling A3 03 0014 93 ep 188 Technical specifications A 1 System recommendations A Introduction This section describes the following in the UNICORN system e Hardware recommendations e Software recommendations e Network recommendations Hardware recom The table below describes the recommended hardware for a UNICORN system mendations Component Recommendations PC Compaq PC Pentium 4 2 5 GHz or higher Memory e 256 MB RAM for one system e 512 MB RAM for two or more systems Hard disk 500 MB available hard disk space Monitor Color monitor 1024x768 pixels small fonts 64K colors Controller e CU 900 PCI requires 1 2 length PCI slot e CU 950 USB requires USB 1 1 port e CU 950 Advanced requires a 10Mbps network interface card Note AKTAprime and AKTAxpress instruments do not connect to the PC through a control ler AKTAprime is connected direc
127. onnected to the Ethernet connection of the computer CU 950 USB If a CU 950 USB controller is installed the settings cannot be changed and always have the following values e Data recovery OFF e Method behaviour PAUSE The illustration below shows the parameter of the Data recovery instruction Note In this case there is only one parameter and it has the same name as the instruction Data recovery Parameters Data recovery Data recovery ON Method behaviour Communication loss CONTINUE OFF ON Data recovery parameter If the connection between the PC and the CU 950 is lost during a method run and Data recovery is set to e ON the run data is saved in the CU e OFF the run data is lost System settings 7 Note You cannot set the parameter to ON unless there is a memory card installed in the CU Method behaviour The illustration below shows the parameter of the Method behaviour instruction instruction Method behaviour Parameters Data recovery Communication loss Data recovery ON z Method behaviour CONTINUE C PAUSE Communication loss CONTINUE Communication loss parameter If the connection to the instrument is lost during a method run and Communication loss is set to e CONTINUE the method run will continue to execute until end is reached e PAUSE the method run will pause When the connection is reestablished you have to click Continue to resume the run
128. ons on page 115 e Choose Administration System Setup in the UNICORN Manager Select the system with problems in the dialog box and click the Edit button Check that the strategy computer name and the control unit number are correct according to the installation at the local station which is physically connected to the system See 6 2 1 System definitions on page 115 e If you connect remotely to a system check that the local station which is physically connected to the system is turned on check that the network is functioning at both the remote and the local station e Check that the limit of eight connections to the system has not been exceeded ep 179 8 Troubleshooting 8 3 Advice on operation 8 3 In this section Cannot perform Quit or Logoff Monitor signals do not appear in the Curves pane in System Control 03 0014 93 ep 180 Methods Advice on operation Methods This section describes the following method scenarios e Cannot perform Quit or Logoff e Monitor signals do not appear in the Curves pane in System Control e Error message Couldn t create result file Destination path could not be found e The Method System Connection dialog box keeps appearing e The Method Editor window does not fit on the screen e There are red instructions in a method e After Windows logout and login you cannot get a system connection e The Print screen command does not send a copy o
129. or the result file is closed Data is also saved to disk at pre set intervals during a run thus minimizing data loss in the event of power or communication failure The capacity of the data buffer is 16000 points for up to sixteen monitors Note The data buffer capacity is listed in the Curves group of System Settings in System Control module If a buffer is filled during a run i e 16000 points have been recorded the number of points is halved by deleting every second point leaving 8000 points on the chromatogram For subsequent sample points every second point is dropped thus halving the effective sampling frequency When the chromatogram again reaches 16000 points the process is repeated and the effective sampling frequency is halved once again Now every fourth sampling point will be recorded Note The real sampling frequency the sampling frequency of the card never changes The initial effective sampling frequency for each monitor is set in the system strategy It can be viewed and changed in the Curves group of System Settings in System Control Note For AKTAxpress systems the sampling frequency cannot be viewed or changed The table below describes the resolutions that apply for the curves at an initial effective sampling frequency of 10 samples per second 10 Hz Duration Card Effective Number of Resolution minutes sampling fre sampling fre points seconds point quency Hz quency Hz 107
130. ormation Strategy and Template files System Installation Manuals Description This component allows you to set parameters Change for your connected system a Space Required 6757 K Space Available 2671792 K lt Back Cancel e p67 2 Installation 2 3 Software Installation 2 3 3 How to install selected software components e Ifyou select a component in the list and click the Change button more detailed installation options are displayed Each component is described below this table e Click the Next button when you have selected the components to install e Depending on which components you selected to install there are additional steps to complete Note These additional steps are described in 2 3 1 How to install UNICORN on page 36 and 2 3 2 How to install UNICORN for AKTAxpress on page 54 Program Files To re install the UNICORN program files e select the component Program Files in the Select Components dialog box Note This will not affect any existing method or result files in the system The program is always installed locally even in network installations User Information The Select Sub components dialog box e Select the User Information check box in the Select Components dialog box e Click the Change button Result The Select Sub components dialog box is displayed Select Sub components J x Select the components you want to install clea
131. ote Connection management is the same for stand alone and network installations System connections 3 3 2 How to connect a system Instruction The table below describes how to connect the System Control module to a chromatography system Note This instruction does not apply to AKTAxpress systems For information on how to connect an AKTAxpress system see the AKTAxpress User Reference Manual Step Action Open a System Control window that is not currently in use It is identified by the Not connected text in the status bar Select System Connect or click the System Connect toolbar icon ra Result The System Connect dialog box is displayed The System Connect dialog box lists the systems to which you have access Select a system to which you want to connect and click OK System Connect fd Explorer1 00 OK Cancel Help Note See additional information about remote and local stations below If you connect If you connect from a remote station to a chromatography system you should be aremote sta aware of the following e The local station the computer physically connected to the system must be logged on to the network e The UNICORN drivers must be running on the local station e The UNICORN program does not have to be running on the local station e p75 3 System connections 3 2 How to connect a system If you connect from a local sta tion 03 0014 93 e p76
132. ox and click the Change button Result The Select Sub components dialog box is dis played showing the sub components e Global Procedures e Global Report Formats e Global BufferPrep Recipes e Global Columns e User File Select the components you wish to install If sub components already exist If any of the above components already exist from a previous installation you will be asked if you want to replace them with the new default files e Normally you should answer No and keep the ex isting files Note See also 2 3 3 User Information on page 69 where this dialog box is described in more detail Installation 2 Component Description Strategy and Template Select the Strategy and Template files check box files for each new strategy that is installed usually when a new system is installed on a local com puter Each strategy needs only to be installed once since they are stored on the server e Click the Change button to display the Select Sub components dialog box There you can select which components to install Strategy files and or Tem plate files System installation The System check box must be selected for worksta tions where systems are connected but not on remote or demo stations Manuals e Select the Manuals check box if you want to install the UNICORN manuals e Click the Change button to display the Select Sub components dialog box There you select additional system manual
133. p 168 The hysteresis setting not available for AKTAdesign systems for a warning determines the extent to which the signal can oscillate around the warning limits without re activating the warning After the signal has activated a warning the warning will not be repeated as long as the signal remains within a window defined by the hysteresis setting above and below the warning limit This prevents repeated warnings from noisy or oscillating signals close to the warning boundary See the illustration below Warning limit gt nysteresis a Ge a x2 WARNING WARNING Note Hysteresis is only relevant for warnings since an alarm puts the system in Pause at the first alarm 7 3 The Specials set tings Specials settings System settings 7 The figure below shows the Specials settings which are available in the Instructions dialog box in the System Control module x r Instructions FracParameters Parameters eon FracParameters DelayVol 0 000 10 000 F TubeChange Tube fe 000 ml Specials Peak_FiacParametersUV Mode Slope TubeChange C Minwidth 0 150 min fe S YW Mae Level 5 000 mAJ Tuba StartSlope 100 000 mAU min EndSlope 75 000 mAU min Keyboard Clone Mode KeyLocked Set Selected Parameter To Strategy Default Value The Specials settings include for example instructions for fraction collectors Note How to open and use the Instructions dialog box is described in 7 1 The Instruc
134. p in the UNICORN Manager Result The System Setup dialog box is displayed e Select the system and click the Edit button e Check that Computer name is the same as the Windows computer name If not change the UNICORN computer name Click OK and then the Close button ep1l15 6 Administration 6 2 System administration 6 2 1 System definitions Possible actions in Systems are edited in the System Setup dialog box in the UNICORN Manager the System Setup dialog box 03 0014 93 ep116 e Choose Administration System setup in the UNICORN Manager to open the System Setup dialog box see illustration below Note To use this menu command you must have Audit trail System setup access see 6 3 2 Access items on page 142 System Setup Systems on System 1 8 System 2 i System 3 Strategy Summary Socket cose Help Possible actions The table below describes the actions you can perform in the System Setup dialog box Each action is described further on in this section Note You need the UNICORN installation CD to perform the actions New and Delete If you want to create a new system definition edit a system definition delete a system definition delete a strategy view or print a system summary use socket communication view installed systems as icons view installed systems in a list then click the button New Edit Delete Stra
135. pansion card e Choose Administration Controller Settings in the UNICORN Manager Result The Controller Settings dialog box is displayed x Select System System Settings Controller cu 900 PCI Control Unit fi System 2 03 0014 93 ep 28 Installation 2 How to install the The table below describes how to install the CU 900 PCI drivers when the hardware drivers the PCI card has been installed Step Action e Start and log on to the PC Result If this is the first time you start the PC after the hardware installation the Found New Hardware Wizard should start e Select the option Install the software automatically e Click the Next button e Select Search for the best driver in these locations e Select only Include this location in the search e Insert the UNICORN CD into the CD ROM drive of the PC e Click the Browse button and locate the folder Drivers Cu900pci e Click the Next button Result The necessary files are copied to the hard disk Note In Windows XP a warning may be displayed saying that the drivers are not signed Just choose the option to install anyway The Completing the Found New Hardware Wizard screen is displayed e Click the Finish button to complete the installation ep29 2 Installation 2 2 Hardware installation 2 2 3 How to install CU 950 2 2 3 When to use CU 950 The LEDs on CU 950 CU 950 USB and CU 950
136. r is logged on to the network before UNICORN is started so that the strategy file on the server disk is accessible 8 2 In this section Unable to access certain UNICORN func tions Connection scen arios Troubleshooting 8 Advice on operation UNICORN access This section describes the following UNICORN access scenarios Unable to access certain UNICORN functions Connection scenarios Connections are not available System is not available Error message in a network installation You cannot control the system Run data Connection in System Control displays a NO 1 NO 2 or NO 3 Scenario UNICORN functions to which you do not have access appear grey in the menu and cannot be used The Manual menu commands in the System Control are grey that is you can establish a connection but cannot control the system Solution Choose Administration User Setup in the UNICORN Manager to change the user profile e Check that no other user has a control mode connection e Check that you have access rights to control the system manually Scenario The connections are not available The connections are not available even though e the connection between the PC and chromatography system appears to be correct e the power is turned on A system is not available when you attempt to establish a connection Solution e Check the connect
137. r the components you do not want to install Sub components Global Procedures Global Report Formats 1276 K Global BufferPrep Recipes 113K Global Columns 1327 K Users File 231K m Description This component includes all files containing global procedures Space Required 60327 K Space Available 1126208 K e Select the check boxes for the components you want to install The components are described below 03 0014 93 ep 68 Strategy and Tem plate files Installation 2 Global information files e Global Procedures e Global Report Formats e Global BufferPrep Recipes e Global Columns If you select the above items you will be asked if you want replace the existing global information files If you click Yes any additions made to these items in the existing installation will be backed up When you start UNICORN the first time after the new installation you will be asked if you want to restore these additions Users file e Users File User definitions passwords and access rights for existing users Methods Results If you select the above item you will be asked if you want replace the existing users file If you click Yes e the default user will be re installed All the users defined in the system including users installed from other stations in a network installation will be deleted and cannot be restored afterwards e any new methods and results created in the exist
138. rdware on page 25 to check if any hardware installation is necessary Note Hardware installation for AKTAxpress is described in the AKTAxpress Installation Guide In this section This section contains these topics Topic See When to install hardware 2 21 How to install CU 900 PCI 2 2 2 How to install CU 950 2 2 3 03 0014 93 ep 24 2 2 1 Pre installed sys tems When hardware installation is ne cessary Installation 2 When to install hardware In most cases your system is pre installed that is it is installed by authorized personnel from Amersham Biosciences If your system is pre installed no hardware installation is necessary Hardware installation is only necessary for a PC which is directly connected to one or more systems Whether this PC is connected to a network or not stand alone installation does not matter In other words if your system is not pre installed and the computer is directly connected to a chromatography system you must install hardware ep25 2 Installation 2 2 Hardware installation 2 2 2 How to install CU 900 PCI 2 2 2 When to use CU 900 PCI Instrument capa city How to install the PCI card 03 0014 93 ep 26 How to install CU 900 PCI The CU 900 PCI expansion card is used to connect chromatography or synthesis instruments to a PC CU 900 PCI can be used together with all the KTA instruments except AKTAprime and
139. ress NEXT to continue Options IV Install strategy IV Install template Strateqy template Have Disk lt Back Next gt Cancel x Follow the instructions in the table below to install strategy and template files 1 2 Action In the Options field select only Install strategy In the Type drop down box select Xpress to install strategies from the installation CD Floppy A to install strategies from a diskette You can also use the Have Disk button for this see step 4 below 3 Select the strategy that corresponds to your system in the Strategy template list box ep 63 2 Installation 2 3 Software Installation 2 3 2 How to install UNICORN for AKTAxpress Step Action 4 e Click the Install button or e click the Have Disk button to locate the strategy files if they are stored on a separate CD or diskette Result The Enter Information dialog box is displayed 5 Enter Information xj Enter a name for the strategy lt Back Cancel e Either keep the suggested default name for the strategy to be in stalled or type another name e Click the Next button A confirmation dialog box is displayed I x es i Strategy and templates installed e Click OK Result The Strategy and Template Installation dialog box is displayed again 7 Click the Next button to continue to the next installation step 03 0014 93 ep 64 Installation 2 S
140. rive type the name of the folder and click OK Note The folder is created in the default location on the se lected drive for example C UNICORN Local Fil and not in the root directory e select the Folders item e select the check box for the new folder e Click OK 6 ep 155 6 Administration 6 3 User Administration 6 3 4 How to assign user properties The Instructions item 03 0014 93 ep 156 Open the User properties dialog box see the table in the beginning of this section and select the Instructions item Instructions B System 1 E 2 Manual V Pump M Flowpath V Alarms amp Mon v v v v Frac Other Calibration V PS60Flow P960Press P900Press P950Press P950Flow Cond_Calib Temp pH Cond_Cell her End method Save partial Local control only Remote control only View only ound Alarm Warning gt m IN NOUS 9 KKK KK KK w ERN JS K The table below describes the subitems of the Instructions item Subitem Determines which manual instructions should be available to the user Calibra which monitors the user should be allowed to calibrate tion Other e ifthe user should be allowed to end a method End method or save a partial run Save partial e how the system should be available to the user Local control only Remote control only or View only Sound which events that should render a sound Differ
141. rmation to start copying the program files If you want to review or change any settings click Back If you are satisfied with the settings click Next to begin copying files Current Settings UNICORN 5 01 Software Setup Full Version Company Amersham Biosciences System Type 2 AKTAxpress System Global Report Formats v 4 gt lt Back Cancel The table describes how to start copying the program files from the CD Step Action 1 The setup program is ready to copy the files The Start Copying Files dialog box displays all the selections that have been made and the components to be installed Note If you want to make any changes you can click the Back button one or more times 03 0014 93 ep62 Installation 2 Step Action If the settings are correct click the Next button to copy the files Step 10 Strategy The Strategy and Template Installation dialog box is displayed if you chose to install and Template In stallation strategy and or template files in Step 4 Select Components e If you did not choose to install strategy and template files go to 2 3 2 Step 11 System Installation on page 65 Strategy and Template Installation Select the strategy you want to install and press the INSTALL button If your strategy template is located on a floppy or on another source press the HAVE DISK button to locate it When all strategies templates are installed p
142. rogram Options screen is displayed e Specify the number of System Control Windows you want to be available normally the number of instruments that will be simul taneously connected to the PC e Click the Next button The Start Copying Files screen is displayed e Click the Next button The System installation screen is displayed e Select the radio button corresponding to the system you want to delete e Click the Change button to display the System Setup screen e Click the Clear button to clear all the fields e Click OK to go back to the System Installation screen e Click the Next button e Click the Finish button Note You can see the screen images in 2 3 1 Step 11 System Installation on page 53 How to deletea The table below describes how to delete a strategy strategy f Note You can only delete strategies that are not currently in use Step Action 1 Select Administration System setup in the UNICORN Manager Result The System Setup dialog box is displayed 03 0014 93 ep 122 Administration 6 Step Action Click the Strategy button Result The Strategy Administration dialog box is displayed xl Strategy Strategy used on system How to view or You can view and print a total summary of a selected system from the System Table print a system Summary dialog box summary The table below describes how to view and print an information summary of a selected system
143. s for global audit trail files Logon Logoff e all logon and logoff attempts e the name of the user logging on or off e failed logon attempts Define system system definition deletion and definition Method Queue Method Queue start operations 03 0014 93 ep 126 Administration 6 Item Displays Maintenance e maintenance activities that are scheduled in the System Control module menu command Sys tem Maintenance e settings made in maintenance module Information e system lock unlock e socket on off e global log started stopped Timezone the time zone where the activity was logged The System tab The illustration below shows a System tab of the Audit Trail dialog box ET atBed Fle Edt View Help Global Bi TheSystem BF Oligo system BE MtpTest BF MutiSwat BF pa 4 gt 2002 04 23 0805 44 W Europe Daylight Time Method makus Result v WNiklas markus001 ses 2002 04 23 030610 W Europe Daylight Time Manual ended on System WINCDRN LAB 21 2002 04 23 0213 17 W Europe Daylight Time Method markus Result v Niklas markus002 1es V Run End Manual Alarms Wamings 2002 04 23 O08 1412 W Europe Daylight Time Manual ended on System WINCORN LAB 21 I Erat 23 04 2002 08 46 26 W Europe Daylight Time Method markus Restt v Niklas markus00G res 2304 2002 03 48 08 W Europe Daylight Time Manual ended on System WINCORN LAB 21 I Calibration 25 04 2002 08 2
144. s independent of the user s logon password A locked system can also be unlocked with the logon password for a user with Unlock locked systems access This access item should be re stricted to a small number of users to prevent indiscriminate unlocking of locked systems e Click OK If you log off or If you log off or quit UNICORN it will automatically disconnect all connected quita system systems and the Leave control dialog box will be displayed for each system Systems that are disconnected in this way will be re connected automatically when you log on to UNICORN again 03 0014 93 ep 82 Calibration 4 4 Calibration Introduction This chapter introduces some calibration concepts and describes how to calibrate monitors for AKT Adesign systems Calibration of monitors is important for the monitors to display correct results In this chapter This chapter contains these topics Topic See Calibration facts How to calibrate monitors for AKTAdesign systems 4 2 ep 83 03 0014 93 4 Calibration 4 1 Calibration facts 4 1 Introduction To calibrate mon itors from differ ent manufacturers from UNICORN Calibrate system modules in the System Control Calibration meth ods One point calibra tion ep 84 Calibration facts Certain system monitors mainly pH monitors need to be calibrated regularly for correct results Most monitors can be calibrated from UNICORN t
145. s to install By default the available manuals of interest to your system will be installed e Select the check boxes for the components you want to install Note For a stand alone installation all components must be selected e Click the Next button to continue ep4l 2 Installation 2 3 Software Installation 2 3 1 How to install UNICORN Step 5 Select In the Select Drive dialog box you choose the installation folder for the UNICORN Drive software Select Drive Step Action Select the disk drive where the program is to be installed This should be a physical disk drive usually C on the computer where you in stall UNICORN not a network disk drive e Click the Next button to continue e Click the Yes button if asked whether Setup should create the UNICORN program folder 03 0014 93 ep 42 Step 6 Setup Type Step 6 Stand alone installation Installation 2 The Setup Type dialog box is displayed Setup Type Select the type of installation you prefer then press NEXT The network installation will setup the software to work over a network Check Remote only system if no systems will be physically connected to the computer A remote only system have to be installed to an existing UNICORN server The stand alone installation will setup the software to work locally on the target system Check Demo system if no systems will be physically connected to the computer
146. ser change instruction 158 change instruction 159 User properties edit the user definition 151 define the advanced items 152 Index e pxi Index define the advanced items 153 define access to folders 154 define access to systems 154 print user setup information 157 User Properties dialog box usage 152 usage 153 User properties dialog box open 149 User properties assign 149 User Setup dialog box 139 open 139 User Setup dialog box 146 open 146 User setup information print 157 Users and folders delete 161 Users file 68 Users own attributes change 160 Users own passwords change instruction 159 V View mode connection 77 View older audit trails 128 W Warning limit 166 03 0014 93 p xii
147. ss systems e 4 systems of other type than AKTAxpress Network installa The list below describes some basic facts about network installations tions Cn e Systems must be locally linked to a workstation which is linked to the network In other words the systems are not directly linked to the network e Each local workstation can be connected to a maximum of 12 AKTAxpress systems 4systems of other type than AKTAxpress e A network can support up to 99 chromatography systems which are connected locally to the workstations in the network e A workstation can locally or remotely control up to four chromatography systems This is achieved using the four possible System Control windows in UNICORN which are available on each workstation e Each chromatography system in UNICORN can be controlled by only one active System Control window can be viewed by up to eight System Control windows ep 191 A Technical specifications A 3 Data sampling A 3 Data storage Data buffer capa city Effective sampling frequency Initial effective sampling fre quency Resolution at 10 Hz sampling fre quency 03 0014 93 ep 192 Data sampling Data from chromatography system monitors is stored temporarily in data buffers in the local system controller Data is transferred from the buffers to disk storage by UNICORN whenever a chromatogram is closed that is when the New_Chromatogram instruction is issued
148. ss to the system see 6 3 User administration on page 137 Note The responsible should be one person or a small group at least in larger installations Network administra maintenance of the network functions relevant to tion UNICORN see 1 Network setup on page 3 Note In a network installation this is normally carried out by the computer staff respons ible for the company s network Actions before the When UNICORN has been installed the administrator must perform the actions peat oben in this table before other users can use the program Step Action Set up system definitions for the chromatography systems see 6 2 1 System definitions on page 115 2 Define new users with home folders access groups and access pro files see 6 3 User administration on page 137 Note The above actions can be performed from any station in a network installation The administrator must be logged on to the network from the workstation so that the changes apply globally throughout the network e p113 6 Administration 6 2 System administration 6 2 Introduction In this section 03 0014 93 e p114 System administration This section describes mainly e how to create and edit system definitions e how to view edit and save log files of the UNICORN system activity e how to back up and restore the system definitions This table describes the contents of this section Topic See System
149. ssible reason The file USERS30 MPM in the folder UNICORN SERVER FIL could be corrupt No user names Remote station Both these conditions must apply e The User name drop down box in the Logon dialog box is empty You are trying to log on from a re mote station in a network installa tion No user names Local station The user list on a local station in a network installation is not up to date Solution e Restore the file USERS30 MPM from the latest back up copy OT e reinstall the default user Make sure that the computer is logged on to the network before you start UNICORN Note A remote station accesses the user list directly from the network server Make sure that the computer is logged on to the network before starting UNICORN Note The user list is stored locally on a local station and is up dated automatically from the network server if the com puter is logged on to the net work Scenario Stand alone installation If you receive the error message Strategy file error in a stand alone in stallation the strategy file is probably corrupt Network installation In a network installation the error message Strategy file error may appear if you try to create a method for a system not physically connected to the computer Solution Reinstall the strategy as described in 2 3 3 How to install selected software components on page 67 Make sure that the compute
150. stallation after the UNICORN software has been installed Step Action e Insert the UNICORN CD in the CD ROM drive Result The Setup wizard starts and displays the Welcome screen e Click the Next button The Select Components screen is displayed e Make sure that the System Installation option is selected You should deselect the other check boxes unless you want to install other components e Click the Next button The Program Options screen is displayed e Specify the number of System Control Windows you want to be available normally the number of instruments that will be simul taneously connected to the PC Maximum value is 1 for CU 950 USB 4 for CU 950 Advanced e Click the Next button The Start Copying Files screen is displayed e Click the Next button The System installation screen is displayed e Go to 2 3 1 Step 11 System Installation on page 53 for further instructions ep 33 2 Installation 2 2 Hardware installation 2 2 3 How to install CU 950 CU 950 Advanced The TCP ports used by UNICORN for the CU 950 Advanced are numbered port numbers 60X01 60X33 where X is the CU ID minus 1 Example e The CU ID 1 default e gt X 1 a 1 0 gt The TCP port numbers used on the PC and on the CU 60001 60033 03 0014 93 ep34 Installation 2 2 3 Software Installation Introduction The UNICORN software is normally pre installed by a Amersham Biosciences representativ
151. t gt Cancel Follow the instructions in the table below to install strategy and template files Select the items you wish to install in the Options field Note Ifthe options for both strategy and template are checked the template files are installed automatically together with the strategy Select your system type in the Type drop down box Select the strategy template that corresponds to your system in the Strategy template list box e Click the Install button or e click the Have Disk button to locate the strategy template files if they are stored on a CD or diskette Note If the strategy and template files are located on different diskettes the setup program will ask for the template diskette when it is needed Result The Enter Information dialog box is displayed ep47 2 Installation 2 3 Software Installation 2 3 1 How to install UNICORN Step Action Enter Information xj Enter a name for the strategy lt Back Cancel e Either keep the suggested default name for the strategy to be in stalled or type another name e Click the Next button An Information dialog box is displayed i x es i Strategy and templates installed e Click OK Result The Strategy and Template Installation dialog box is displayed again e Repeat steps 1 to 6 in this table if you have more than one system configuration and want to install additional strategies Note
152. tegy Summary Socket Icons List Note The Socket button is described in 1 2 If named pipes cannot be used on page 7 Administration 6 How to createa The table below describes how to create a new system definition new system defini ne tion Note When the system definition has been created remember to grant access to the system to the appropriate users Step Action e Insert the UNICORN CD in the CD ROM drive Result The Setup wizard starts and displays the Welcome screen e Click the Next button The Select Components screen is displayed e Make sure that the System Installation option is selected You should deselect the other check boxes unless you want to install other components e Click the Next button The Program Options screen is displayed e Specify the number of System Control Windows you want to be available normally the number of instruments that will be simul taneously connected to the PC Maximum value is 1 for CU 950 USB 4 for CU 950 Advanced 4 for CU 900 PCI e Click the Next button The Start Copying Files screen is displayed e Click the Next button The System installation screen is displayed e Go to 2 3 1 Step 11 System Installation on page 53 for further instructions How to edit a sys The table below describes how to edit the parameters of an existing system tem definition detiiiion Step Action 1 Select Administration System setup
153. tep 11 System The System Installation dialog box is displayed if you chose system installation in Installation Step 4 Select Components System Installation j x Number of connected AKTAxpress systems 2 x Strategy to use jax 00_w0p11 System unit namefs Unitt Syst Unit fSys2 Unit 3 D Unit P uis i ame a Unit 7 a Unit 8 a Urits os ttf uif m oros Action Specify the Number of connected KTAxpress systems 1 12 This is the maximum number of systems that you plan to connect simultan eously to the computer Note The number you choose here determines how many sys tems you can name in step 3 in this table Choose the Strategy to use for the systems Note The strategy you choose will apply to all systems you cannot define different strategies for different systems To change strategies after the UNICORN installation choose Administration System Setup iz the UNICORN Manager Type the names for the systems in the Unit 1 12 edit fields Click the Next button Note If you want to define systems later or change the settings for a previously defined system run the Setup program once again with only the System Installation option selected in the Component Selection dialog box e p65 2 Installation 2 3 Software Installation 2 3 2 How to install UNICORN for AKTAxpress Step 12 Setup The installation is complete and the computer must be restarted Complete
154. than the path in dicates click the Browse button to select the correct folder Note Select A if the file is located on the diskette e In the Items field select which information to include from the backup file e Click the Restore button to restore the system definitions Note You can click the Information button to see which inform ation files are included in the backup file Administration 6 6 3 User Administration Introduction Access to the UNICORN software is controlled by username and password authorization This is done from within the UNICORN program where each authorized user is assigned to an access group that determines which functions the user can perform In this section The table below describes the contents of this section Topic See How to delete users and folders 6 3 6 e p 137 6 Administration 6 3 User Administration 6 3 1 User access groups 6 3 1 User access groups Introduction This section describes e the purpose of access groups and access items e how to view and edit the access groups e suggested responsibilities for some typical access groups Kinds of access A UNICORN installation has 10 different access groups with different rights to groups perform actions in the UNICORN system Some access groups are predefined while others are undefined The table below describes the different kinds of access groups Kind of access group Description
155. the Cancel button or the Exit button If you do this however the installation will be incomplete and the software cannot be used e After the installation the installed files are compared with the original files on the installation CD to make sure that no files have been corrupted during installation The criteria used for the file comparison are name size version and checksum Installing a new version of the UNICORN software over an existing UNICORN installation is no problem You do not have to uninstall the previous version before installing the new version Note If you have made additions to the previous installation you will be asked if you want to keep these additions when UNICORN is started the first time after the new installation This is described in 2 3 1 Step 4 Select Components on page 41 UNICORN is supplied on a CD ROM Files on the CD ROM are compressed and cannot simply be copied onto the hard disk During the installation procedure the required folder structure is created on the hard disk and the files are decompressed Do not attempt to decompress the files using any other file decompression utility Follow the instructions in the table below to begin the installation Action For network installations log on to the network and check that you have access to the server disk and folder where the UNICORN net work components are to be installed e p37 2 Installation 2 3 Software Installation
156. ther user whose access should be restricted e Verify that the restrictions apply to the user How to restore The table below describes how to undo the local policy changes previously the local policies described Step Action Log on to the computer as a real administrator 2 e Delete the file Registry pol from the folder C WINNT System32 GroupPolicy lt user gt in Windows 2000 or C WINDOWS System32 GroupPolicy lt user gt in Windows XP Note lt user gt is the identity you used to log on in step 1 Log off from or restart the computer Result Another default Registry pol file is created by the Windows File Protection system e Log on to the computer again with the same identity 3 Open your local security policy e Click Start Run and then type gpedit msc Result The Group Policy window in displayed 4 e Inthe tree view select the folder User Configuration gt Administrat ive Templates gt Windows Components gt Windows Explorer e Inthe right window pane double click the setting Prevent access to drives in My Computer e Click the Policy tab e Click the Not configured radio button e Click the Apply button and then OK e p 107 5 Security 5 5 Single application mode Step Action e Close the Group Policy window e Log off from the computer e Log on to the computer as the same administrator Log off from the computer 7 Repeat steps 1 to 5 for each of the users
157. tion e Start the UNICORN program on all local stations in a network installation if possible and establish a view mode connection as an indication that a connected system might be running 9 9 Introduction Single application mode on com puter level Single application mode on user level The instructions in this section Security 5 Single application mode To restrict access in Windows How to restrict unauthorized access to certain files and folders in the UNICORN application was described in 5 1 Access security on page 90 Still users can access these files and folders through the Windows Explorer unless Windows is configured to prevent this e This is the reason for using single application mode which restricts access in Windows Definition Single application mode means that UNICORN is the only application available when it is running The user cannot open or switch to other programs Single application mode is normally set through the Options dialog box in the UNICORN Manager module see 5 6 The Options dialog box on page 109 The setting is then valid for the computer and will apply to everyone who starts UNICORN on the specific computer Single application mode can also be applied to individual users instead of computers This section describes how to configure Windows to start in single application mode for individual users This means that instructions will have to repeated for each user the m
158. tion User Setup in the UNICORN Manager when you want to display the User Setup dialog box See illustration below Go Define users and their security attributes m Access groups Each user is assigned to an access group This group defines access to different functions in UNICORN When changing a group all users assigned to that group are affected m Users Name Full name Group default default Administrator mm OPC user OPC user Administrator Edit Delete Print LEP To view the access groups you must have User setup Groups access See 6 3 2 Access items on page 142 for an explanation of the access items The table below describes how to view the access groups and their properties Step Action 1 Choose Administration User Setup in the UNICORN Manager Result The User Setup dialog box is displayed e p 139 6 Administration 6 3 User Administration 6 3 1 User access groups Step Action Click the Access groups button Result The User properties dialog box is displayed LI x Access groups Admmistrator Access Power user Group User al procot operator UNICORN Manager ue st Process supervisor V Method Editor Group 7 V System Control Group 8 v Evaluation Group 3 User setup Groups Group 10 Audit trail System setup V Delete move Home only V Unlock locked system vV Run methods v Manual interactio
159. tions dialog box on page 164 e p 169 7 System settings 7 4 Monitors settings 7 4 Monitors settings The Monitors set The figure below shows the Monitors settings which are available in the Instructions tings dialog box in the System Control module sstem monitors Instructions i x r Instructions Wavelength Parameters Aan uvi 190 700 280 ren C Specials uv2 190 700 Monitors s Hmm I OFF uv3 190 700 K jas rm M OFF C Curves CeeneFactne 2 zi Set Selected Parameter To Strategy Default Value The instructions and parameters depend on the selected monitors Note How to open and use the Instructions dialog box is described in 7 1 The Instructions dialog box on page 164 03 0014 93 p170 1 9 Introduction The Curves para meters Curves settings System settings 7 This section describes the Curves settings which are available in the Instructions dialog box in the System Control module xl Instructions UVI Parameters C Aems Store Time between samples 0 100 s C OFF ON C Specials uv2 Store ON Time between samples a C Time between samples 0 100 s 0 100 Monitors W3 z Store ON Time between samples 0 100 Cond O Store ON Curves Tima hahunan tamniae N ANN e zi Set Selected Parameter To Strategy Default Value Note How to open and use the Instructions dialog box is described in 7 1 The Instructions dialog box on page 164 Th
160. tly to the serial port on the PC and AKTAxpress is connected to the PC via USBcan II Drives CD ROM drive Peripherals Mouse Printer The printer which is delivered together with the sys tem from Amersham Biosciences Note New printer models are added on a regular basis and therefore cannot be specified be forehand ep 189 A Technical specifications A 1 System recommendations Software recom mendations Network recom mendations 03 0014 93 ep 190 The table below describes the recommended operating systems for workstations running the UNICORN software Item Recommendations Operating system for Microsoft Windows 2000 SP4 or higher the workstations or e Microsoft Windows XP Professional SP1 or higher The table below describes the network recommendations for UNICORN ina network installation Item Recommendations Network server Microsoft Windows 2000 Server Network protocol TCP IP Services Server and Workstation Note The last two points ensure that named pipes are usable over the network and that folders can be connected to a drive unit Technical specifications A A 2 UNICORN control capacity Introduction This section describes UNICORN s capability to control systems in stand alone installations and network installations Stand alone install In a stand alone installation the computer can be connected to a maximum of ations 7 e 12 AKTAxpre
161. tor 950 Sample pump P 950 Sample pump P 960 AutoS ampler 4 900 AutoS amplerT hermo AutoS ampler 4 905 ADSOD Nine eee ee MOCO zi Cancel Help Note The strategy contains instructions for all possible compon ents but only instructions for the selected system compon ents will be shown K K K U U K K K K K K K JI e Select the check boxes for the components you want to add to the system and click OK Caution If you make changes to a system you may also have to change the arrangement of tubing pumps columns etc on the sys tem An attempt to control a system using the wrong strategy may cause malfunction and ultimately damage the system Click OK and then Close How to deletea The table below describes how to delete a system definition system definition A i o Note A system definition can only be deleted if the system is idle and no users are connected to the system e p 121 6 Administration 6 2 System administration 6 2 1 System definitions Step Action e Insert the UNICORN CD in the CD ROM drive Result The Setup wizard starts and displays the Welcome screen e Click the Next button The Select Components screen is displayed e Make sure that the System Installation option is selected You should deselect the other check boxes unless you want to install other components e Click the Next button The P
162. tructions in the table below to install UNICORN UNICORN ona workstation Step Action 1 Install UNICORN See 2 3 1 How to install UNICORN on page 36 select the Network installation option or 2 3 2 How to install UNICORN for KTAxpress on page 54 03 0014 93 ep 20 Network setup 1 Step Action Reboot the PC and log on as one of the domain users that will run UNICORN Connect the shared folder again This is necessary since connected network drives are user specific Open Windows Explorer Select Tools Map Network Drive to connect the shared folder on the UNICORN file server to the designated drive letter Start UNICORN and set up Select the drive letter in the upper drop down list box Make sure the Reconnect at Logon option is selected Click the Browse button Locate and select the shared folder UNICORN will use and click OK Click the Finish button the system definitions see 6 2 1 System definitions on page 115 the user profiles see 6 3 User administration on page 137 ep2l 2 Installation 2 Installation Introduction This chapter describes how to install e hardware external controller or expansion card e software UNICORN software It also describes e system connection management e system monitor calibration In this chapter This chapter contains these sections Topic See Installation overview Hardware installation Software installation How to
163. twork server in a network installation An Information dialog box is displayed e Click OK e Click the Next button e Click the Finish button System Installa In the Select Components dialog box select the System Installation check box tion e if you want to install a new or an additional system on a stand alone computer or a local station in a network e if you want to change the settings for an existing system Note Systems are not installed on demo or remote only systems ep71 2 Installation 2 3 Software Installation 2 3 3 How to install selected software components Manuals 03 0014 93 e p72 The table below describes how to install the Manuals Action e Select the Manuals check box if you want to install additional manuals or re install the manuals Note This will not affect any existing method or result files in the system The manuals are installed locally even in network installations e Click the Change button Result The Select Sub components dialog box is displayed Select Sub components xj Select the components you want to install clear the components you do not want to install Sub components UNICORN ATML manual Basic Optional Configurations 18 1174 45 OK Basic User Manual 18 1140 76 ad_ pdf OK AD 900 Instructions 56 3140 38 aa pdf OK J Air sensor Air 900 Instructions 56 3050 85 OK P w Description Space Require
164. ubject to Amersham Biosciences Standard Software End User License Agreement Copyright Amersham Biosciences AB 2004 All rights reserved Table of Contents Table of Contents 1 Network Setup iciicieccccctceecusnen Sacstessucenbeanscaiccanseanbeanedeaen Saeaeneeubeenedita seeenteceniastenadiaresaseistemenee 3 1 1 Network terms and CONCCHISs 4 oi siindssuveras cdecyi as centlece ga exten onuteenduunetecsawaad erste aneren 4 1 2 NEIWOFK CTIVILONMAICT bss xcuetrancsmcaiatardavewiaystuaneetahyaSrantanisesudinaw au Tes teats radteonmanetteedin 7 1 3 UNICORN enwork exain ple zicciess cwiteies entire saatouy cue ttrt aan enned 8 1 4 How to configure the network Servel cccssssssceeeeeeessssseeeeeeeeeesseeeeeeeeeeeeseeeeeeeeeeseaeaes 9 1 4 1 How to configure Windows 2000 Servel cccccccccseecseeeeeeneeeseeeeseneseneeeeeenaees 10 1 5 How to configure the WOrkstations ccccccsssssescseeeesssssseeeeeeeseeeesseeeeeeeeesseeeeeenensees 12 1 5 1 How to configure Windows 2000 workstatiOnS c ccccceeeseeeeeeeeeeeeeeeeeeeeeeaees 13 1 5 2 How to configure Windows XP workstatiONnS ccccccceeeseeeneeeeeeeeeeeeeeeeeeeeeeaees 17 2 Inst llati on n sateen initiate nis 22 2 1 Installation Overview axciiuxen sania ierateestaheereneauneacse E EE ET a 23 2 2 Hardware 1nStallatl Otiiecsiccrass ccucntetestcuua cone chncteuasarhdwaneatcuntsantasacursarhuaseueasnmaumatens 24 2 2 1 When tO Stall HardWare octctsdcatartad
165. w e p119 6 Administration 6 2 System administration 6 2 1 System definitions Step Action 3 cont Computer name The Computer name should be the same as the Windows name of the computer that the system is connected to Normally this is the local computer e Control unit number Select the control unit number 1 4 This is the connection number for the system on the local computer CU 900 PCI The control unit number is set with the DIP switch see 2 2 2 How to install CU 900 PCI on page 26 CU 950 USB Must be installed with the control unit number 1 CU 950 Advanced Please contact your local Amersham rep resentative e Auto save interval UNICORN saves a copy of the result file every five minutes during arun This minimizes loss of data in the event of a computer failure The interval cannot be changed e Component See step 4 below 03 0014 93 ep 120 Administration 6 Step Action Some system strategies allow you to define the specific system com ponents included in the system e Click the Component button to specify the system components e Ifthe Component button cannot be clicked go to step 5 Result The Component dialog box is displayed I x Select components Column alve 2 Column alve Y3 Outlet Valve Y4 SampleValve V5 Buffer alyeAl V6 FlowDirection alve Y7 Valve Y8 Valve V9 Switch valves Cond monitor pH Cell Fraction collec
166. words You cannot use the user name as password except for the default user The Advanced settings in the User properties dialog box determine the expiration time for passwords Passwords should be changed regularly by the users even if the user profile is set up without a password expiration time A user which has User setup Groups access usually a system administrator can change the password for any user The table below describes how to change the password for a selected user Step Action Choose Administration User Setup in the UNICORN Manager Result The User Setup dialog box is displayed Select the user in the list and click the Edit button Result The User properties dialog box is displayed e Select the User item in the dialog box See 6 3 4 The User item on page 151 for an explanation of the User item e Type the new password in the Password and Confirm fields 4 Click OK and then the Close button How to change the attributes for a selected user Administration 6 A user which has User setup Groups access usually a system administrator can change the attributes for any user The table below describes how to change the attributes for a selected user Action Choose Administration User Setup in the UNICORN Manager Result The User Setup dialog box is displayed Select the user in the list and click the Edit button Result The User properties dialog box is displayed e Se
167. work Identification Wizard starts Click the Next button Select the This computer is part of a business network and I use it to connect to other computers at work radio button and click the Next button Select the My company uses a network with a domain radio button and click the Next button Result The Network Information dialog page is displayed Read it and click the Next button Type a User name Password and Domain This user must be a domain administrator Click the Next button Type a Computer name and Computer domain Click the Next button Enter User name Password and Domain This user must be a domain administrator Click OK Add the displayed user to the computer and click the Next button Click the Finish button Restart the computer The last steps in the network setup are e to map the shared folder to a drive letter e to install the UNICORN software See the instructions below Follow the instructions in the table below to map the shared folder to a suitable drive letter Log on to the Windows 2000 workstation with domain user rights This user must also be a member of the user group created before Select Tools Map Network Drive to connect the shared folder on the UNICORN file server to the designated drive letter epl15 1 Network setup 1 5 How to configure the workstations 1 5 1 How to configure Windows 2000 workstations How to install UNICORN ona workstation 03
168. ype of controller System name System 1 System type Chromatography KTA z Strategy E100F400 ba Controller Settings CU 950 USB Control Unit fi CU 950 Advanced IP address CU 900 PCI 192 168 O 1 Help Default Clear cea e In the Controller field select the CU 950 Advanced option e Type anew System name if you want to change the suggested name e Choose the System type and Strategy e Choose the Control Unit number 1 4 e Type the IP address of the CU 950 controller Note IP Address IP Address Mask and IP Gateway Address must be set manually for the CU 950 controller when the CU 950 Advanced option is chosen Contact your local Amersham Biosciences representative for more information e The Default button sets the default resource settings for the selected system type e The Clear button clears all fields if you want to remove a system e When you are finished click OK e p51 2 Installation 2 3 Software Installation 2 3 1 How to install UNICORN 03 0014 93 ep 52 Step 3c Action System Setup for CU 900 PCI expansion card System Setup xj Set the system parameters for the selected system Set the system name type and the strategy that shall be used for the system Select and setup the installed type of controller System name System 1 System type Chromatography KTA E Strategy E100F400 ha Controller Sett

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