User Manual
Contents
1. Recorded P 2 at 1496 1580 No Name Surface cma OIV 601 OIV6O2R OIv602a OIV603R OIVEORX OIV604R JOIV604 OIV605R OIVv60 amp OIVv606R JOlV L OIV611R JOlV lilL JOIVSIR JOlV i OIV613R jOIV61X ON GRALED v2 03 Copyright c 2014 LAAZ Bt Registered to BCE Sz l szeti Tansz k Figure 6 10 Data appear in the lower table 2 GRALED v2 03 C sample jpg olm Start Stop A Next point F8 Zoom 25 Recorded P 2 at 1496 1580 Surface ema OIV 601 OIV602R JOIV6OA OIV603R j OIv603X OIV6048 JOIV604 OIV605R OIv60 amp OIVv606R JOlV L JOIV LIR JOlV llL JOIVSIR jOIv61X OIV613R jOIV61X3 ON RALED v2 03 Copyright c 2014 LAAZ Bt Registered to BCE Sz l szeti Tansz k Figure 7 11 You have the possibility to measure further samples with opening a new image or stop measurements after a single sample 12 Click SAVE to export table data 2 GRALED v2 03 CAsample jpg olim Start Stop Next point F8 Zoom 25 Recorded P 2 at 1496 1680 o o o o o o o o o o o LII en No E p edo 601 pr soar pez pes p ex or soa for sos pss es ory eoa ow sue pre preis for sia jo 613R es fon GRALED v2 03 Copyright c 2014 LAAZ Bt Registered to BCE Sz l szeti Tansz k gt Figure 8 Landmark points first ramification of N3 irst ramification of N irst ramification of N f f first ramification of N3 f f i2. is freely distributed AS IS in the hope that it will be useful but WITHOUT ANY WARRANTY without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE Citing GRA LE D Please refer to this software in your publication as GRA LE D version 2 04 Corvinus University of Budapest Hungary 2014 You can download from e http www laaz hu en software graled developer szoleszet uni corvinus hu index php id 56232 researchers It is highly appreciated if you cite this software or any publication announced on the software website How to install Installing GRA LE D is quite easy Download the zip compressed package and save it on your hard disc or flash drive then extract and open GRA LE D executable How to use GRA LE D Sampling and digitalization collect your leaf sample and scan it even with an ordinary desktop scanner on 300 dpi recommended value Save images using bitmap BMP or JPEG JPG format Position your samples consequently with the upper side up or down Such uniform set of images is easy to handle for any purpose It is recommended to place your sample in the scanner with petiole up Processing method is not sensitive to rotation or other adjustment variations but uniformity makes batch processing much easier Please note that precise measurements could be carried out with some criteria e leaf has to be intact without any damage e flat the leaf as much as it is possible e ma
3. middle third of several shoots on both halves of the leaf distance from petiole sinus to upper lateral leaf sinus Mature leaf length petiole sinus to lower lateral leaf sinus ENN d To be measured on at least 10 mature leaves from the Description middle third of several shoots on both halves of the leaf distance from petiole sinus to lower lateral leaf sinus Mature leaf angle between N1 and N measured at the first ramification Code Nos OIV 601 and OIV 602 Description To be measured on at least 10 mature leaves from the middle third of several shoots on both halves of 10 leaves the angle between N4 and N measured on the tangents formed before these veins first branch OIV code w Mature leaf angle between N and LAN measured at the first ramification Code Nos OIV 601 and OIV 602 Description To be measured on at least 10 mature leaves from the middle third of several shoots on both halves of 10 leaves the angle between N and N measured on the tangents formed before these veins first branch Mature leaf angle between N and N measured at the first ramification Code Nos OIV 601 and OIV 602 Description To be measured on at least 10 mature leaves from the middle third of several shoots on both halves of the leaf the angle between N and N measured on the tangents formed before these veins first branch Mature leaf angle between N3 and the tangent between pe
4. senescence of the leaves or even phytotechnical treatments When you reach to missing point simply presses F8 and skip it Later You have to handle missing data in statistical analysis In the present example Figure 10 landmark point 13 is missing In this case you reach and record landmark point 12 and press F8 two times and indicate landmark point 14 In your exported data all missing coordinates and missing OIV values will be indicated with N A Figure 10 Biometric points given by the software refer to the following OIV descriptors Mature leaf length of vein N Description To be measured on at least 10 mature leaves from the middle third of several shoots length of vein N OIV code OIV602 Mature leaf length of vein N To be measured on at least 10 mature leaves from the Description middle third of several shoots on both halves of the leaf length of vein N OIV code OIV603 Mature leaf length of vein N To be measured on at least 10 mature leaves from the Description middle third of several shoots on both halves of the leaf length of vein N3 Mature leaf length of vein N 5 To be measured on at least 10 mature leaves from the Description middle third of several shoots on both halves of the leaf length of vein Na Mature leaf length petiole sinus to upper lateral leaf A sinus To be measured on at least 10 mature leaves from the Description
5. 6L p 611R 611 Jo 612R 612 613R p 613 jon GRALED v2 03 Copyright c 2014 LAAZ Bt Registered to BCE Sz l szeti Tansz k Figure 3 Red cross also appear on the picture so that you can check position In case of false positioning click again on the correct landmark point Coordinates will be updated automatically Press F8 for next and click to 2 landmark point On the top of the table on the left you can check which point you are currently reading 2 GRALED v2 03 CAsample jpg olm DPI 300 Start Stop Next pont F8 Zoom 25 Recorded P 2 at 1496 1580 eo e o e o e o o o o o No Name surface mdomo forsor foiveoa orveosx foweox oweo foweoa Jorv6osR owveox foiveosr fowveon omeim Jove foe owsa foweur oweix fon GRALED v2 03 Copyright c 2014 LAAZ Bt Registered to BCE Sz l szeti Tansz k Figure 4 Go on until you finish with the 32 landmark points and Table fill with coordinates Figure 5 2 GRALED v2 03 CAsample jpg 5 0 0 0 0 0 0 0 0 0 0 0 No Name Surface cm4OIV 601 OIV6O2R OIv602 OIV603R OIVEORX OIV604R JOIV604 OIV605R joro OIV606R JOlV L JOIVSLIR JOlV llL JOIVSIR OIVv6i1X OIV613R jOIV61X On GRALED v2 03 Copyright c 2014 LAAZ Bt Registered to BCE Sz l szeti Tansz k Figure 5 9 When all points are defined press ANALYZE 2 GRALED v2 03 CAsample jpg olm Start Stop Next pont F8 Zoom 25
6. GRA LE D GRApevine LEaf Digitalization Version 2 04 User Manual L szl Baranyai amp P ter Bodor Corvinus University of Budapest Budapest Hungary 2009 2015 Whati GRA LED T a aa aaa aaa 3 EIN OR ME t 3 HOWTO STAL astar cS 3 ONTO e GRALE D MT 3 Landmark ere Br E T onto aeons 9 TOUDE NOONE aien A tet do Eee pci hU A 10 Biometric points given by the software refer to the following OIV descriptors 11 NON OIV descriptors provided by the software ccccsssccccessececeesecccceesececseeceeeeesceeseuecetsuneceesenseeetes 18 What is GRA LE D GRA LE D GRApevine Leaf Digitalization is free raster graphic software exclusively built up for digital analysis of Vitis leaves based on the standard ampelographic measurements published by OIV International Organization of Vine and Wine 2 Edition of the OIV descriptor list for grape varieties and Vitis species During morphological description of grapevine cultivars have discovered that leaf provides numerous data which could be statistically analysed For facilitating objective description we created a free software which we think may help in this process We appreciate your feedback any suggestions or ideas are welcome and please feel free to send us your questions about the GRA LE D software Contact us at laszlo baranyai uni corvinus hu bodor peter uni corvinus hu This program
7. ative Overlapping lobes measure at the widest overlap between the blades The value will be recorded as positive NON OIV descriptors provided by the software NON OIV Distances between the points descriptors Overlapping To handle overlapping you should make a marking on the leaf before scanning as the following schedule suggest Flat the leaf with overlapping on the petiole sinus Mark the overlapping with color marker Replace the overlapped lobes Supplementary figure 20
8. pening overlapping of petiole sinus E To be measured on at least 10 mature leaves from the middle third of several shoots Wide open sinuses measure the distance between Description the blades at half the height of the petiole sinus The value will be recorded as negative Overlapping lobes measure at the widest overlap between the blades The value will be recorded as positive Mature leaf opening overlapping of petiole sinus DT E To be measured on at least 10 mature leaves from the middle third of several shoots Wide open sinuses measure the distance between the blades at half the height of the petiole sinus The value will be recorded as negative Overlapping lobes measure at the widest overlap between the blades The value will be recorded as positive Mature leaf opening overlapping of petiole sinus E To be measured on at least 10 mature leaves from the middle third of several shoots Wide open sinuses measure the distance between the blades at half the height of the petiole sinus The value will be recorded as negative Overlapping lobes measure at the widest overlap between the blades The value will be recorded as positive Mature leaf opening overlapping of petiole sinus E To be measured on at least 10 mature leaves from the middle third of several shoots Wide open sinuses measure the distance between the blades at half the height of the petiole sinus The value will be recorded as neg
9. rkoverlapping Overlapping modify your results in two ways give false result of the leaf area and make difficulties when you measure opening of the petiole sinus See chapter OVERLAPPING Measurement Before start print the supplementary figure to follow biometric points in the GRA LE D 1 2 Start GRA LE D and open a file from your hard disc or flash drive with OPEN TD GRALED 201 a Open No Name Surface omPOlv 601 wen joven jom om maa otv com pra pres jor cos jore wes ren pues jor sum eta Jovem A GRALED v2 01 Copyright c 2014 LAAZ Ex Registered to BCE Sz l szeti Tansz k Figure 1 Click on OPEN and choose your file from your hard disc or flash drive with the selection of file type Bitmap bmp or JPEG jpg D N ewr m uU a EI A m v Bitmap bme hd JPEG jpg Figure 2 Zoom out and adjust zoom for proper marking of the points Press START STOP 2 times 5 Start marking the 1 biometric point with click on the 1 landmark point on your sample Coordinates of the point appear in the right table gt GRALED v2 03 CAsample jpg a DPI 300 Next point F8 Zoom 25 Recorded P 1 at 1380 1748 bi al al alol nisi al al O Open Analyze Save Exit No p eov 601 jov 602R jov 602 603R 603 ev 604R 604 605R ov 605L p 606R p 60
10. rst ramification of N 25 top ofthe teeth of the first ramification No top of the teeth N 14 Jjbaeofthesns SS 5 7 1 1 1 1 1 1 1 1 1 1 2 2 2 2 0 1 2 3 4 5 6 7 3 9 0 1 2 3 4 5 base of the teeth N top of the teeth Ns base of the petiole Export data You can export your data in tab delimited plain text TXT file format to your hard disc This structured data can be imported to spreadsheet applications and statistical softwares In the text file sample name will be the same as the picture filename was Troubleshooting 1 Following dialog bar appear Figure 9 You did not select one or more biometric point of the leaf 2 Your exported txt file is empty Figure 9 You forgot to press ANALYZE after the measurements 3 During the data analysis you have outliers Your sample set was not uniform or you made mistake during the measurements 4 After finish with the marking of 32 points you still have empty cell in the table You forgot to press F8 in one or more times during the measurements and coordinates are overwritten If you miss one point or marked a wrong position correct the mistake by pressing F8 until you reach the point needs to be replaced 5 You have missing landmark point on your sample You should aim to collect intact samples without any damages but it not always come true It is usually happens that one or more parts are missing on the sample caused by hail
11. tiole point To be measured on at least 10 mature leaves from the Description middle third of several shoots on both halves of the leaf angle between N3 and the tangent between petiole point and the tooth tip of N5 Mature leaf Mature leaf length of veinNS 0 of vein N5 To be measured on at least 10 mature leaves from the Description middle third of several shoots on both halves of the leaf length of vein N5 mm Mature leaf length of tooth N2 To be measured on at least 10 mature leaves from the Description middle third of several shoots on both halves of the leaf length of tooth of N2 mj S Mature leaf width of tooth N2 To be measured on at least 10 mature leaves from the Description middle third of several shoots on both halves of the leaf width of tooth of N2 mm Mature leaf length of tooth N4 To be measured on at least 10 mature leaves from the Description middle third of several shoots on both halves of the leaf length of tooth of N4 mj SI Mature leaf width of tooth N4 To be measured on at least 10 mature leaves from the Description middle third of several shoots on both halves of the leaf width of tooth of N4 Mature leaf length between the tooth tip of N2 and the tooth tip of the first secondary vein of N2 Description Measurement on at least 10 mature leaves from the middle third of several shoots on both halves of the leaf Mature leaf o
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