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1. User Manual TN2085 Cryostat Semi Automatic Cryostat T e AN IN E R Read Prior to Operation Always keep this manual near the unit scientific WP47113 06 02 15 TANNER User Manual scientific TN2085 Cryostat 888 708 5233 Info TannerScientific com www TannerScientific com Contents 1 General Summary Page 2 2 Safety 5 sss 3 3 Technical Parameters esters 4 4 Installation suspende 5 5 Equipment Overview 6 6 Operation 12 6 1 Main Control Panel Page 12 6 2 Turning the Instrument On 13 6 3 Turning the Instrument On 13 6 4 Peltier Element 13 6 5 Temperature Setting 14 6 6 Time Setting 14 6 7 Programming the Working Time for Each Day 15 6 8 Programming the Defrost Cycle 15 6 9 UV Sterilization Lamp 16 6 10 Stand By 16 6 11 Illumination 16 6 12 Use of Liquid Waste Container 16 6 13 Specimen Forward amp Backward Feed 16 6 14 Section Trim Thickness Selection 17 6 15 Right Side Control Panel 17 7 Cleaning amp Maintenance 18 8 Warranty amp Service 19 9 Troubleshooting sss 20 Tanner Scientific TN2085 Semi Automatic Cryostat 1 1 General Summary Model TN 2085 Semi Automatic Cryostat Microtome is a medical device used for rapid freezing of pathological sections of human and animal tissue It can be widely used for pathological diagnosis analysis and research in hospitals medical colleges and by legal me2. C 3 For high quality sectioning pay attention to the following e Proper temperature in the chamber e Correct operation and hand wheel speed e Fine adjustment of the anti roll plate e Sharp blade edge correct blade angle Hand Wheel Lock ZI LOCKING ROTARY SPANNER LEVER Tanner Scientifice TN2085 Semi Automatic Cryostat 11 6 Operation General Overview LEFT SIDE CONTROL PANEL 1 POWER SWITCH RIGHT SIDE CONTROL PANEL GLASS WINDOW LIQUID WASTE BOTTLE c Sa lt KT gt mom mn gt STABILIZING LEGS gt MOVING HANDLE HAND WHEEL 6 1 Main Control Panel e The main control panel is separated into 4 parts From left to right they are the Freezing Shelf Area the Freezing Chamber Area the Time Setting Area and the Special Function Area FREEZING FREEZING TIME SHELF AREA CHAMBER AREA SETTING AREA SPECIAL FUNCTION AREA SUN MON TUE WED THU FRI SAT Hi ac B opono 12 Tanner Scientific TN2085 Semi Automatic Cryostat RIGHT SIDE CONTROL PANEL 6 2 Turning the Instrument On Turn on the power switch on the right side of the instrument The cryostat will power up and begin cooling On initial start up the unit will automatically go into standby mode only cooling to the default standby temperature of 9 C to 10 C Turn off the standby mode
3. Press again to turn off oj ILLUMINATION 6 12 Use of Liquid Waste Container Use waste barrel to collect defrosted liquid tissue thawing solution flushing liquid and waste objects In order to prevent both human and environmental hazard from the waste put 200mL 1096 formalin or other sterile solution into the barrel in advance The disposal of the waste shall follow all the related regulations of the hospital NOTE Please use the corrected and effective method to treat the waste 6 13 Specimen Forward and Backward Feed Specimen forward and backward feed is for fast movement of the specimen position If you press the 1 button the specimen goes to home position in normal speed If you press the i button the specimen goes forward in normal speed If you press the tt button the specimen moves to home position in fast speed Pressing the H button moves the specimen to the opposite direction in fast speed Press the H button again to cancel moving command 16 Tanner Scientific TN2085 Semi Automatic Cryostat SECT TRIM S T SECTION TRIM SECTION DISPLAY Spm M RIGHT SIDE CONTROL PANEL 100 50 b VACUUM EXTRACTOR 6 14 Section Trim Thickness Selection Above the LED displays the thickness value of section trim If SECT lights up LED displays section thickness otherwise itis trim thickness Press S T button to change from
4. despite correct temperature and correctly aligned anti roll plate Blade and or anti roll plate is dirty Edge of anti roll plate is damaged Blunt blade Clean with dry cloth or brush Replace plate Use different part of the cutting edge or replace Sections curl on the anti roll plate Anti roll plate does not protrude far enough beyond the cutting edge Readjust correctly Ridged sections Damaged blade Edge of anti roll plate is damaged Use different part of the cutting edge or replace blade Replace plate Chatter during sectioning Specimen insufficiently frozen onto the specimen disc Specimen disc not clamped tightly Blade not clamped tightly enough Specimen has been sectioned too thickly and detached from the disc Blunt blade Blade profile unsuitable for the specimen to be cut Incorrect clearance level Refreeze specimen onto the disc Check disc clamping Check blade clamping Use different part of the cutting edge or replace blade Use blade with different profile Set correct angle Sections tear Temperature too low for tissue cut Blunt part dust dirt frost or rust on the blade Leading edge ofthe anti roll plate is damaged Hard particles in the tissue Increase temperature and wait Remove cause from blade Replace plate Flattened
5. Failure to follow this procedure could cause frosting and icing in the chamber e Do NOT place instrument directly in sunshine or near air condition vents Placement e The instrument is a movable floor model with 4 caster wheels It also features two stabilizing legs in the front that are adjustable to level the cryostat e The instrument must be transported in an upright position When tilted the compressor oil is displaced and can cause problems Hand Wheel Assembly e Insert the pin of the hand wheel shaft into the hole of the hand wheel e Mount the spring washer on the screw M10x15 e Tighten the screw with a 6 Allen key Freezing Chamber Assembly e Place the waste tray in the freezing chamber e Install blade holder on the microtome base and lock it in e Place all tools needed for specimen preparation in the chamber e Close glass window Connect Power Check your power socketto ensure itis well grounded and that the power supply is stable constant and accurate Compressor requires a start up current between 45 and 50A Tanner Scientific TN2085 Semi Automatic Cryostat 5 5 Equipment Overview HEAT EXTRACTOR Freezing Shelf The freezing shelf has 20 stations and is located at the left of the freezing chamber The freezing shelf can drop as low as 40 The two project stations in the front of the freezing shelf are Peltier stations and they can drop to 60 The procedure for preparing sp
6. Wait 15 minutes e Wipe chamber off with tissue paper or dry cloth e Reprogram temperature according to slicing requirement after disinfection Blade Holder Cleaning If the blade holder sticks and or contains impurities then dismount and clean as follows e Turn adjustable handles in the lateral of the rotator and draw out sideways e Push the knife clamp back which has knife clip and shift it out from rotator e Turn rod handle in the lateral of the knife cap and draw out sideways e Clean all the assemblies of the blade holder e Dry off all assemblies and apply a drop of low temperature lubrication oil e Reassemble blade holder and put into the freezing chamber 18 Tanner Scientific TN2085 Semi Automatic Cryostat 8 Warranty amp Service Tanner Scientific warrantees this product for a period of one year from the date of purchase provided that the customer complies with the rules ofthis manual Extended warranties are available Contact Tanner Scientific at 888 708 5233 for more information Tanner Scientific will not be responsible for the damage and other problems caused by abuse and misuse of the instrument Service Information If you require any service or parts during the warranty period please contact Tanner Scientific at 888 708 5233 or the sales agent who sold you the unit Prior to calling please have the instrument model and serial number ready Amendment Tanner Scientific reserves the right t
7. and minute Press the KEY button again to resume normal operation NOTE If the setting time is over 10 seconds between two operations the system will automatically return to normal state 14 Tanner Scientific TN2085 Semi Automatic Cryostat 6 7 Programming the Working Time for Each Day While in the idle state ta and e indicating setting mode is unlocked press the button to enter programming mode The LED will display as Image A indicating a working time setting mode Press D H or M to select the day and time the instrument should start running Press the button again to enter switch off mode to set up the date and time The instrument should stop running and stay in the idle state Image SUN MON TUE WED THU FRI SAT SUN MON TUE WED THU FRI SAT AM AM Hoh Hi o B 0000008 nono IMAGEA IMAGE B NOTE User can press W DAY to setup a group of days at A once By pressing the W DAY key all the days of the week will be set to the same ON and OFF times FAST DEFROST UV STERILIZE 6 8 Programming the Defrost Cycle Press A key button then press EA in the time control area When the frost indicator light comes on you can adjust the defrost settings The indicator lights up for ALL days of the week This is because the defrost cycle runs atthe same time each day You can adjust the time by pressing the hour button H and minute button M Select t
8. point 4 At this stage the Peltier element may be deactivated by pressing the Peltier button again Once deactivated the display indication returns to PE Pressing the button during the Peltier element working process will stop the Peltier freezing immediately Tanner Scientific TN2085 Semi Automatic Cryostat 13 6 5 Temperature Setting The compressor will start to work after the power switch is turned on This allows the freezing chamber to cool down The freezing chamber temperature is displayed and adjusted in the temperature set area The LED displays the temperature of the sensor in the freezing chamber Freezing Chamber Temperature Display amp Settings System Default is 22 C When the TN 2085 Cryostat is powered up and standby mode is off press A to increase the temperature and M to decrease the temperature The unit s LED will flash to display the set value When set to the desired temperature wait about 15 seconds and the instrument will return to its normal state 6 6 Time Setting C Y SUN MON TUE WED THU FRI SAT PISE EAT PANEL BUTENG SUN Sunday AM Morning AM MON Monday PM Afternoon TUE Tuesday W DAY Working Day PM WED Wednesday D Day THU Thursday H Hour FRI Friday M Minute LA o nk After turning on the system verify that the default time is correct or reset it Press the KEY button to access the setting mode Press the D H or M button to adjust the day hour
9. than 10 3 Make sure the edge ofthe plate and the edge ofthe blade are flush A EDGE OF PLATE AND BLADE MEETING FLUSH 1 The upper part of the anti roll plate should not contact the tissue specimen 2 When the quality of the tissue section is not good check the edge of the blade and check any segment of tissue on the plate 3 Avoid touching the front of the plate ANTI ROLL because high temperature will adhere PLATE HEIGHT the tissue section to the plate TURNING KNOB 10 Tanner Scientific TN2085 Semi Automatic Cryostat Section Reference 1 When using the cryostat s microtome controlling the blade speed and correctly adjusting the anti roll plate are important factors in cutting high quality sections The proper speed ofthe blade can only be acquired by practice and experience along with skillful control ofthe hand wheel The proper adjustment of the anti roll plate requires even more practice Sometimes they interfere with each other and require adjustment 2 When freezing tissue water within the tissue will condense into ice and the tissue will become hard The hardness changes with the temperature The lower the temperature the harder the tissue becomes Different tissue reacts in various ways under differenttemperatures Achieving high quality sections can only be obtained through practice Cutting temperatures of specimen tissue without fat and formalin should be between 13 C 23
10. to disable it and the indicator light will turn off simultaneously Tanner Scientific TN2085 Semi Automatic Cryostat 17 7 Cleaning Maintenance These machines are precision instruments Pay attention to cleanliness maintenance and repair work Do NOT use organic solvents or any aggressive substances to clean and sterilize the chamber Only use the cleaning agents specified in this manual such as alcohol or common disinfectants based on alcohol It may be necessary to conducta manual defrost before cleaning if the chamber is full of frost If necessary the external painted surface may be cleaned with light duty commercial household cleaner Use a wet cloth and rub until dry Do NOT apply xylene or cleaning solutions that contain alcohol Ex glass cleaner Daily Cleaning of Freezing Chamber Conduct the following steps before cleaning each time e Remove all the specimens from freezing chamber e Lock the hand wheel e Remove the blade from the blade holder and putitback in the blade box e Activate the vacuum to clean away scraps e Empty the waste tray e Use alcohol or common disinfectants to clean up baffle and brush shelf e Collect the waste cleaning liquid from the barrel and dispose of it Spray Disinfection The cryostat must be disinfected after each daily use The steps to ensure the machine is adequately cleaned are as follows e Cool down freezing chamber to 20 e Spray with disinfectant e
11. alfunction contact a qualified service technician Do NOT try and solve the problem yourself Power Supply e A professional electrical engineer should inspect the electrical circuit before installation e The instrument uses a single phase AC power supply the voltage is 110 115 11V If the power does NOT meet this requirement then a 2000 3000VA AC voltage regulator shall be connected for usage e A dedicated 20AMP circuit is required e Confirm the power supply is grounded triple socket then connect the instrument with the main power socket Turn on the power switch in the back of the instrument to start the machine Temperature e The instrument s working ambient temperature is 5 28 C High temperature or humidity will influence freezing effect and shorten compressor life Operating e The blade is sharp exercise extreme caution when handling it e NEVER TRY TO CATCH A FALLING BLADE e Before changing specimens always lock the hand wheel and cover the blade edge with the blade guard Make sure the hand wheel is in the locked position when the instrument is NOT in use e Avoid contact with cold parts of the instrument Tanner Scientifice TN2085 Semi Automatic Cryostat 3 3 Technical Parameters Section Thickness Range 1 60um Adjustable Increment 1um Increment 20 C Trimming Thickness Range 10 300um Adjustable Increment 10um Increment 20 C Specimen Retraction 20um Horizonta
12. by pressing the pu key The unit will now cool to user s set temperature Allow unit to completely cool for a minimum of 12 hours from ambienttemperature This will insure the entire chamber and microtome is at set temperature 6 3 Shutting Down the Instrument It is advisable the unit be properly shut down to avoid excess stress on the refrigeration system The compressor should not be running when you turn off the power switch Press the AL button to increase temperature by 5 C and wait until the compressor shuts off Turn off the power switch on the right side ofthe instrument LEFT SIDE Important Before restarting the unit make sure the CONTROL entire chamber and microtome are completely dry PANEL Otherwise ice will form and may cause damage to the microtome assembly Advancement retraction may not be obtained due to freezing of the step motor a 6 4 Peltier Element Under a normal state the Peltier element is off The LED shows the freezing shelftemperature When you press the Peltier button the indicator light comes on and the Peltier element begins to work to cool down the freezing stations The LED displays L15 which means the Peltier element will work in 15 minutes The countdown of the remaining cooling time is now permanently displayed The Peltier element turns off automatically after 10 minutes Once the remaining cooling time displayed shows 4 minutes the number four is followed by a
13. dical experts It utilizes advanced horizontal feeding and the specimen chamber area is much larger than competing models Instrument Consists of 4 Major Parts e The computer control panel is on the upper part of the instrument This area displays the temperature and the general working conditions of the instrument e The low temperature chamber is used to rapidly freeze the tissue and to conduct cutting e The compressor cooling condenser and evaporator are located in the lower part of the instrument e The microtome assembly is located inside the chamber A MAIN CONTROL PANEL B LEFT SIDE CONTROL PANEL C GLASS WINDOW D LIQUID WASTE BOTTLE E POWER SWITCH F RIGHT SIDE CONTROL PANEL G MOVING HANDLE H HAND WHEEL 1 STABILIZING LEGS Main Control Panel FREEZING FREEZING SHELF AREA CHAMBER AREA TIME SPECIAL SETTING AREA FUNCTION AREA wm fi SUN MON TUE WED THU FRI SAT e AM BOO amp 2 Tanner Scientific TN2085 Semi Automatic Cryostat 2 Safety Precautions This user manual includes important instructions and data related to the operating safety and maintenance of the instrument The user manual is an important part of the product It must be read carefully before using the instrument for the first time and must always be kept with the instrument This cryostat may only be operated by qualified personnel In case of m
14. ecimen is as follows e Cut specimen into the proper size e Place specimen onto freezing disc between layers of tissue freezing medium e Put freezing disc into a station on the freezing shelf e Use heat extractor to flatten the specimen surface e The specimen will freeze quickly e Move the heat extractor away after its frozen e Insert the freezing disc with the specimen into the hole of the clamp on the microtome and then tighten the locking handle e Adjustthe angle ofthe knife holder and blade e Rotate the hand wheel accordingly to cutthe specimen into sections 6 Tanner Scientific TN2085 Semi Automatic Cryostat Microtome Component NOTE Before inserting freezing disc please lock hand wheel securely 1 2 3 6 4 o o gt 1 5 1 Freezing Disc Locking Handle A Extractor Hole 2 Orientation Adjustment Handle 5 Knife Holder Locking Block 3 Orientation Lever 6 Specimen Hole Inserted Freezing Disc Insert Freezing Disc e Release the freezing disc locking handle e Insert the shaft of the freezing disc into the location hole of the specimen clamp Make sure the shaft ofthe freezing disc is fully inserted The entire rear surface of the prism should make contact with the specimen clamp easily there should be no gap between them e Lock the freezing disc locking handle e Observe the cutting angle and if necessary adjust the orienta
15. efore reinstallation Call technical support Specimen disc cannot be removed Moisture on the underside caused the disc to freeze to the freezing shelf or specimen head Apply concentrated alcohol to the contact point or heat the specimen head Cryostat not operational Main plug not properly connected Defective fuses Temperature control switch activated Check that the main plug is properly connected Replace the fuses Reset temperature control switch Tanner Scientific TN2085 Semi Automatic Cryostat 21 TANNER scientific Information The information notes and figures that appear in this user manual represent the present state of knowledge as we comprehend it Due to continuous improvements in technology and manufacturing technique specific and manufacturing procedures may change For inaccurate statements drawings and technical illustrations in this user manual we exclude liability as far as permissible No liability is accepted for any monetary loss or consequential damage caused by or related to compliance with statements or other information in this user manual Tanner Scientific will update the manual as needed according to the latest technological developments This document is protected under copyright laws Any reproduction of illustrations or text requires prompt documented consent by Tanner Scientific Statements drawin
16. g lever on the upper rightto open the blade clearance and then insertthe blade from left to right then lock the clamp lever refer to diagrams of blade holder shown on pg 8 NOTE Blade is sharp be careful when replacing Adjusting the Angle of the Blade Loosen the lowest adjustment handle on the right side ofthe blade holder Refer to the reference scale to turn rotator in orderto choose the best cutting angle of the blade Retighten the adjustable handle Adjusting the Anti Roll Plate The anti roll plate is made of plexiglass Q KNOB FOR CLAMPING ANTI ROLLING PLATE USEA HEXAGONAL WRENCHON BLADE AND SLIDETO MAKE PARALLEL Tanner Scientifice TN2085 Semi Automatic Cryostat 9 Adjusting the Anti Roll Plate includes the following steps 1 Make the edge of the anti roll plate parallel to the edge of the blade e Insert blade and lock the clamping lever e Lock two knobs e Use hexagonal wrench to make blade and anti rolling plate parallel e Fasten hexagonal screw e Turn height turning knob to adjust height of anti rolling plate 2 Adjustthe anti rolling plate angle with the blade ANTI ROLL PLATE TISSUE ADJUSTABLE ANGLE A 700 NOTE The angle 300 should be no La ANGLE OF CUTTING BLADE EDGE more
17. gs illustrations and other information regarding contents or technical details of the present user manual are not to be considered as warranted characteristics of our products These are determined only by the contract provisions agreed between us and our customers Refer to the name plate on the back of the unit for the serial number and manufacturing date of this instrument Distributed by Tanner Scientific 1073 Tallevast Rd Sarasota Florida 34243 888 708 5233 Info TannerScientific com www TannerScientific com TANNER scientific 1073 Tallevast Rd Sarasota Florida 34243 888 708 5233 Info TannerScientific com www TannerScientific com
18. h a cloth Frost on chamber walls and microtome Cryostat is exposed to air flows open windows and doors Sliding window was open and exposed to air flow too long Frost built up by breathing into the cryochamber Change the position area in which the cryostat is installed Ice formations on the bottom ofthe cryochamber Condensation water drain blocked Open the tap of the drain tube turn offthe instrument and allow it to thaw and dry Condensation on anti roll plate and blade during cleaning Brush forceps and or cloth too warm Store all tools on shelf in chamber Anti roll plate damaged after adjustment Plate too high above the cutting edge the adjustment was carried out in the direction of the cutting edge Raise plate when aligning Be more careful next time Tissue sticks or crumbles on the anti roll plate Anti roll plate too warm or incorrectly positioned Static electricity Fat on corner edge of anti roll plate Rusty blade Cool down anti roll plate or reposition plate Remove static electricity source Remove fat with alcohol Remove rust or replace blade Inconsistent or insufficient specimen feed Microtome was not entirely dry when switching on a refrigeration subsequently ice built up in the micrometer feed system Defective microtome Remove the microtome and dry it thoroughly b
19. he time that you would like the defrost cycle to run ES Fast Defrost Button The fast defrost button is located in the special function area Under the normal freezing state itis NOT lit Press E to activate the defrost function The indicator light will go on as the system goes into fast defrost mode The unit should then defrost within a 6 minute time period To cancel fast defrost press the button again The indicator light will go out simultaneously e If system automatically enters fast defrost mode the 52 button lights up too Press it again to stop fast defrost and the indicator light will turn off Tanner Scientific TN2085 Semi Automatic Cryostat 15 6 9 UV Sterilization Lamp Press the UV button to make the lamp active press once more to stop it Indicator light is on or off correspondingly UV UV STERILIZE UV RADIATION IS HARMFUL FOR THE HUMAN BODY When doing UV sterilization do not forget to close glass window and A keep hands away from chamber Before doing UV sterilization please remove all specimens and clean chamber first 6 10 Stand by Press once to enter into standby mode Press again to return to normal a working mode Indicator light is on or off correspondingly In standby mode the freezing chamber temperature is maintained between 4 and 8 to STANDBY conserve electricity 9 6 11 Illumination 101 Press button turn lamp on
20. l Stroke 1 Vertical Stroke 1 75 Specimen Orientation 8 C X Y X Three Directions Voltage 110V 10 15AMP Frequency 50Hz Power 850VA Largest Start up Current 5 sec 45A Chamber Temperature 10 C 35 C Adjustable Freezing Shelf Temperature lt 50 C Number of Freezing Stations 18 Peltier Number 2 Refrigerant R404a 300g 10g Compressor Oil 0 6L EMKARATE RL 22S ICI Dimensions Width w o Hand Wheel 26 7 Width w Hand Wheel 32 5 Depth 27 5 Height 45 3 Weight 278lbs 4 Tanner Scientific TN2085 Semi Automatic Cryostat 4 Installation Transportation Unpacking e Unit must be shipped in the upright position If the unit was shipped or stored lying down it MUST stand upright for 36 hours before use for all lubricating oils to enter the compressor e The instrument should only be used after 4 hours of standing still to ensure proper lubricant distribution e Please inspect the package for damage If it is damaged do NOT open Immediately contact Tanner Scientific and the transportation carrier Site Requirements e Do NOT operate in rooms with or near explosive chemicals e Place instrument at least 30cm from other instruments e Areas on both sides of the instrument should be clear to allow proper air flow to the cooling unit e Do NOT place the instrument in rooms above 75 F or a humid environment The environment s relative humidity should be no more than 80
21. o change the technical parameters of any model for improving the function of our instruments Quality Guarantee e Tanner Scientific will ensure that every instrument sold has been strictly examined to ensure it meets our stringent quality and technical standards e Service terms are only provided for those who regularly use the instrument and operate the instrument according to its instructions Disposal of Unwanted Instruments Discarding the instrument and its parts should be done according to current laws and regulations Tanner Scientific TN2085 Semi Automatic Cryostat 19 9 Troubleshooting SEMI AUTOMATIC CRYOSTAT Section smears Specimen not cold enough Blade and or anti roll plate not yet cold enough Select lower temperature Wait until the blade and or anti roll plate have reached chamber temperature Section splinters Specimen too cold Select a higher temperature Sections not properly flattened Static electricity Specimen not cold enough Large area specimen Anti roll plate is poorly positioned or aligned Incorrect clearance angle Blade is not sharp enough or damaged Remove cause Select lower temperature Trim the specimen parallel to increase the specimen thickness Reposition the anti roll plate align correctly Set correct angle Use different part of cutting edge Sections not properly flattened
22. sections curl up when anti roll plate is picked up Static electricity or air currents Anti roll plate too warm Remove static electricity Cool down the anti roll plate No or insufficient refrigeration Compressor defective Leak in the cooling system Inappropriate site conditions Ventilation slits of the liquefier dirty Call technical support Clean ventilations slits 20 Tanner Scientific TN2085 Semi Automatic Cryostat Thin thick sections Temperature incorrect for tissue cut Blade profile inappropriate for specimen cut Ice buildup in the blade back Hand wheel speed not uniform Blade not clamped tightly enough Specimen holder not clamped tightly Cryocompund applied to cold specimen disc specimen detached from the disc after freezing Blunt cutting edge Incorrect clearance angle Microtome not properly dried before reinstallation Dried specimen Increase temperature and wait Remove cause from blade Replace plate Scraping noise during sectioning and specimen return movement Anti roll plate protrudes too far beyond the cutting edge and is scraping against the specimen Readjust correctly Scraping noise atthe microtome Friction between the slot cover and the microtome housing Apply cryostat oil to the slot cover and distribute by turning the hand wheel or wit
23. selection to trim one time or conversely Section Thickness Range 1 60um default value is 1 20um in 1um steps 20 60um in Sum steps Trim Thickness Range 10 300um default value is 30um 10 60um in 5um steps 60 100um in 10um steps 100 300um in 50um steps Thickness Selection Under current working mode press or to adjust section or trim thickness Section Turn the hand wheel one rotation The system will cuta piece of section one time and the thickness will be displayed NOTE When adjusting specimen movements the hand wheel must be locked in the right position 6 15 Right Side Control Panel The right side control panel consists of counter and vacuum extractor control sections Counter The figure above the LED displays the number of wheel rotations and thickness When Zn lights up the number of rotations is shown in the LED screen When Zum lights up the total thickness is shown Press M button to change from total pieces to total thickness or conversely press C to clear all the numbers Vacuum Extractor Vacuum extractor is designed for cleaning tissue scrap Press button of the right control panel to enable the extractor motor Press once for 3 seconds of the vacuum and press twice for continuous run The indicator light on the upper corner of the symbol comes on Move the speed controller on the left side to adjust the motor speed so you get suitable air volume Press again
24. tion e To adjust orientation Loosen orientation adjustable handle Adjust orientation lever to choose best cutting angle Relock orientation Tanner Scientifice TN2085 Semi Automatic Cryostat 7 Blade Holder Position Adjustment CLAMPING LEVER KY FOR LOCKING BLADE IO o 7 S E CLAMPING LEVER FOR MOVING BLADE HOLDER FRONT AND BACK CLAMPING LEVER TO MOVE BLADE HOLDER LEFT TO RIGHT Se E 4 4 Y E e S 72 CUTTING ANGLE OF BLADE ANTI ROLL PLATE ADJUSTING KNOB ANTI ROLL lt gt PLATE SLIDE ZI LJ IN o e pu CLAMPING S LEVER FOR MOVING BLADE HOLDER 8 Tanner Scientific TN2085 Semi Automatic Cryostat ANTI ROLLING PLATE HEIGHT KNOB Release counter clockwise blade holder lock handle on the left refer to diagrams of blade holder on pg 8 Manually move the holder back and lock itin the desired position When the holder moves to the terminal point the entire blade holder can be taken out and put back by reversing this procedure Release counter clockwise blade holder lock handle on the right manually move the holder left and right and lock it at the desired position NOTE Remember to lock the blade holder to right position when doing forward or backward specimen adjustment Changing the Blade Release the clampin
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