Antigen Plus Version 7.4 User`s Manual
Contents
1. ae l 4 O Test Results may also be entered by keystroke with the exception of 10 on the Marsh scale which must be selected with the mouse To remove a result from a box click once on the box 18 then click on the blank space above the zero in the choices box As the results are entered the exclusions obtained will appear in the Evaluation box at the bottom of the screen If the Shade Ruled Out Antigens on Results Panel checkbox in menu Options is checked the excluded antigens will also be shaded The bottom line of the Results Panel RuleOuts keeps a running tally of the number of times an antigen has been excluded Exclusions are specific to the results column in which they are entered and are so marked The basis for those exclusions is documented below in the Exclusion Policy section Results and exclusions are maintained as long as the selected cells are not cleared If the Selected Cell Panel is saved any results entered against those cells will be saved with the panel and will appear on the Results screen when the saved Selected Cell Panel is loaded If more cells are required for testing than appear on the Results screen simply exit the screen by clicking the Exit button and add cells to the Selected Cell Panel by the Search or Add Lot to Selected Cells methods The current Evaluation box will appear at the bottom of the Search screen Any new cells selected will be present on the Results screen when it is cal2. s additional antigen phenotype You may enter an additional antigen of up to 8 characters in the entry box or select one from the list by clicking the box open To add what is in the entry box to this cell click the Add button To delete an item from the additional antigen display box enter the item you wish to delete in the entry box then click the Delete button To delete ALL the items in the display box click the Delete button and press the Delete key on the keyboard Additional Antigens Add Delete To add what is in the entry box to this cell click the Add button To delete an item from the additional antigen display box enter the item you wish to delete in the entry box then click the Delete button To delete ALL the items in the display box click the Delete button and press the Delete key on the keyboard There is no limit on how many additional antigens you may enter However once the screen space is used up further antigens will be stored but not displayed and only the first 20 can be exported to ASCII files for use in other databases When there are more additional antigens than can be displayed on a Lot Panel print out an ellipsis at the end of the additional antigen list displayed alphabetically will indicate the presence of the additional typings and alert the user to refer to an original hard copy or to a Selected Panel print out or a Selected Cells Panel screen containing that cell to determine the precise
3. 0 RuleOuts 4 3 4825 12 6 8181114 6 3223 8 Evaluation IAT Can Not Exclude E Kpa Lea Lua Enz Can Not Exclude E Kpa Lea Lua These are tentative rule outs Additional confirmatory data based on your own criteria are recommended P1 rule outs 4 need at least 3 negative reactions 6 found for P1 Xga rule outs 3 need at least 3 negative reactions 5 found for Xga The Results screen may be called up from the File menu whenever selected cells are present as a result of selection by search or by adding a lot panel or panels to the Selected Cells in the Utilities menu Results can be recorded as they are read by clicking in the box in the appropriate test column that is in the same row as the tested cell The test column box will be highlighted and a drop down box will appear with a choice of results that may be entered by clicking on the desired number The default choice is O through 4 Agglutination Grading Result choices may be seen and entered in the Marsh O to 10 scale by choosing that option in the Options menu Once a choice is entered move the cursor down to the next tested cell and repeat the procedure If no result was entered in a box the next box will require one mouse click to be highlighted and a subsequent click to bring up the result choices Click in Results Box to Indicate Test Outcome Additional Antigens t O 0 Ch Coi i 0 0 _ CH He 2 l D 3
4. Down Arrow keys while the cursor is in the box The box will be opened and the data will be displayed in numerical and alphabetical order Any item in the list can be selected with a mouse click or by highlighting it with the Up or Down Arrow keys and pressing Enter Alternatively items can be scrolled without opening the box by pressing the Down Arrow key A list box may be opened at a particular spot in the list by first entering a character or characters in the box and then opening it A button may be activated or pushed by clicking it with the left mouse button or by moving the cursor to the button and pressing any key You can tell when the cursor is on a button because its outline becomes darker and if it has writing on it the writing 1s surrounded by a dotted line As with boxes the cursor can be moved from button to button by using the Tab or Shift Tab keys or by clicking directly with the mouse In general the cursor moves from left to right and top to bottom on the screen A donor cell is identified by its Donor ID and Lot Number A donor number or lot number may appear any number of times but the Donor ID Lot Number combination must be unique In order to save a cell in the database it must have a non blank Donor ID and a non blank Lot Number When you are entering several cells from a single antigram or lot panel the data boxes are blanked after you Save but remain indexed at the last item entered Therefore when you e
5. Fy a b Jk a b M N S s D C E c e K Fy a b Jk a b M N S s 1 If the phenotypically matched cell does not react with the patient s plasma select a panel of red cells based on one example of each antigen to be excluded per cell EXAMPLE FOR THE PATIENT LISTED ABOVE THE SELECTION CRITERIA WOULD BE CELL 1 E K Fy b Jk a S CELL 2 E K Fy b Jk a S CELL 3 E K Fy b Jk a S CELL 4 E K Fy b Jk a S CELL 5 E K Fy b Jk a S This will confirm or exclude which antibody ies to the common antigens the patient has formed and what is left to form in the future 11 1f the phenotypically matched cell s is reactive with the patient s plasma and the autocontrol is negative the most probable cause is an antibody to a high frequency antigen Use Antigen Plus to select any examples of high frequency antigen negative red cells in your cell library I e At a k Kp b Js b etc 111 If you have a limited rare cell library or failed to obtain a negative reaction with the high frequency antigen negative red cells available consider that various red cell treatments which modify or destroy certain antigens on red cells can also be useful DTT AET treatment will destroy Kell not Kx Lutheran Knopps M Coy Cartwright Cromer Dombrock Auberger JMH and Lwa Gerbich is destroyed by DTT but not AET ENZYMES papain ficin trypsin bromelin will remove Duffy MNS Chido Rogers JM
6. This is optional information and not normally of significance in the selection of panel cells Group The Group button allows you to enter the blood group of the donor cell Since most commercial panel cells are group O that is the default setting To change the blood group to A B or AB click the Group button until one of those characters appears To leave the blood group unspecified click the Group button until a blank appears Frozen Click the Frozen box to mark a cell as frozen The expiration date will be blanked Frozen cells will be found last in the search process since searching is done starting with newest cells first Antigens The 28 principal antigens are displayed with colored labels for easy identification When you are entering a new cell all the antigens will have a 0 setting You may change a setting to a s w or blank by clicking the button with the mouse pointer right and left mouse clicks scroll through the choices in opposite orders or by moving to a button with the Tab key and pressing any other key A blank setting indicates that no information is available For example some suppliers may not include the Kell system Kp and Js so you may blank these antigens buttons Save Clicking the Save button will save all the cell data displayed for the unique Donor No Lot Number combination Any items not previously present in the data boxes Donor Lot Expiration Date Supplier Additional Antigens an
7. negatives in the Rh Hr antigen group see illustration below If changes are made to that antigen group a red warning flag will appear stating that the phenotype has been cleared This flag will appear upon any user action following the change If that action is to save the cell a dialog box will present the option of canceling the save If the save is cancelled the warning flag will disappear and no warning will be given on the subsequent save action Antigen Plus Ab ID will not save a cell with an inappropriate Rh Hr designation The individual antigen entries may remain but the Rh Hr identification will be erased until it is reinstated at which time the antigens will again be marked with the appropriate pluses and minuses In cases where you wish to designate an RH Hr without knowing the entire phenotype you must enter a qualifying character after the Rh Hr description e g RIR1x to allow the program to accept an incomplete Rh Hr typing An exception is made for electronically imported panels in which a red cell manufacturer has supplied a cell with an Rh Hr designation that is not fully supported by the cell phenotype Note Antigen Plus no longer specifies the Cw antigen in Rh Hr designations since we cannot predict 100 when C will be on the same gene complex as Cw Rh Hr 11 Additional Antigens The Additional Antigens box consists of an entry box an Add and Delete button and a display box directly below them containing this cell
8. on your own criteria are recommended It is the user s responsibility to record accurate test data and to confirm that data according to established protocols 20 Technical Information for Further Testing 1 If all cells in the first panel are reactive and the autocontrol is negative try chemically treated screen cells and see if this alters the reactivity in any way Chemical treatment of red cells includes enzymes DTT AET or CDP chloroquine diphosphate Methods and information on obtaining any of the products required can be found in the American Red Cross Immunohematology Methods and Procedures D Mallory ed Selecting 2 or 3 cells from the panel or using the screen cells will save time save plasma and still provide new information If the information is that nothing changed go to step 2 More information on the actions of specific chemicals is listed in step 2 111 2 If all cells in the first panel tested are reactive and the auto control is negative perform a common phenotype on the patient s red cells patient must not have been transfused in the preceding 90 days Blood group systems to include Rh Kell Duffy Kidd and MNS Select a cell from any of the panels or screen cells in your inventory that is negative for the same antigens the patient lacks and test it This is referred to as running a phenotypically matched cell EXAMPLE PATIENT D C E c e K Fy a b Jk a b M N S s POSSIBLE MATCHES D C E c e K
9. phenotype of the donor cell The reason for the entry box method of adding additional antigens is to ensure consistency you must be consistent in your notation in order for a search to work properly For example to indicate a Colton b antigen Cop as positive you may enter Co b CoBPos or any other notation that is 8 characters or fewer but you should use the exact same notation for all cells with this antigen The entry box will contain a list of all the additional antigens previously saved in your inventory and you should check for an antigen s presence before entering a new one Out Of Stock Click the Out Of Stock button click OK in response to the pop up query and then click the Save button to mark a cell as exhausted The data will remain on file so that you can recall the phenotype in case the cell shows up in a new lot The cell will not be available in the search process unless you mark the Include in Searches feature in the Out of Stock Inventory box in the Options menu When you click the Save button you will be given the option of marking the entire lot as out of stock Click Yes or No in the popup window or type y or n to indicate your choice Please note If you do not click the Save button after marking the cell as out of stock the cell will remain in stock and the out of stock flag will not reappear on the screen when that cell is recalled 12 Sex Clicking the Sex button will change the button from blank to M to F
10. 00000000000000 25 Importne Daliesin neee E E aNs 26 Rare Cell Inv Cito yc scccics nts cecccctecssoes n eee tees 28 ASCH Fille Creati Messrs eana 29 Additional Information esesseececcsseccccessecccccssececccscecccsecccecsssececosecccecsseeceeo 30 Introduction Antigen Plus Ab ID is a red blood cell inventory and antigen search program that allows the user to enter test results that are automatically evaluated to assist with the interpretation of those results Itis designed to permit fast and easy data entry of test panels and then search the data for any combination of antigens you choose It is intended to quickly select donor cells with particular phenotypes for antibody identification studies and to speed up the interpretation process for the blood bank technologist The data stored for each donor are Donor ID Optional Note Lot Number Vial Number Expiration Date Supplier Name Location Phenotype Designation Sex Blood Group 28 Principal Antigens Phenotype Additional Antigens user defined special typings Frozen flag Out of Stock flag The user may specify any combination of antigens to search for search the data and select any cells desired enter test results and print a selected panel or results panel worksheet The user may store and retrieve the selected panels with any test results as desired AntigenPlus Version 7 must be run from inside Microsoft Windows 9x or later It requires a printer that can print in Landscape for
11. 1 added by 7 5 upgrade 02 07 2011 Date 02 07 2011 added by 7 3 upgrade 02 07 201 Last Saved 06 01 2011 EXIT LOAD Delete This Panel Open the Name Number or Date list box by clicking on the appropriate radio button or down arrow You can narrow the search list by first entering a character or characters in the box and then opening it Click on a title and click the Load button This will add the panel you have selected along with any results previously entered to the Selected Cells and Results screens Loading a saved panel will empty the Selected Cells and Results screens of any previously entered data Once a saved panel is loaded additional cells may be added to the Selected Cells and Results screens by using the Search function see page 14 or the Add Lot to Selected Cells function Any time a saved selected cell cannot be found during loading presumably because a user has deleted the cell since having saved the panel the cell will not be loaded with the panel and any results associated with that cell will be removed from the evaluation calculation The following message will appear CELL NOT FOUND ERROR Cell 3242 0511326 not Found It will not be added to your selected panel 25 Import Data This menu option imports data into the AntigenPlus Version 7 database from panel PNL files supplied by email from Rowny Systems or downloaded directly from the Rowny website You may also import entire database
12. Copyright 2013 Rowny Systems Inc Copyright and License Provisions The ANTIGEN PLUS Ab ID program is protected by the copyright laws pertaining to computer software Copies can be made for backup purposes only Itis illegal to give copies to another person or duplicate the software by any other means Purchase of the ANTIGEN PLUS Ab ID CDs and manual from Rowny Systems Inc or one of its distributors constitutes a license to install and use the program on computers or a computer network with three stations in a single laboratory facility ANTIGEN PLUS Ab ID may not be shared by facilities in different geographical locations unless each facility is individually licensed Warranty Rowny Systems Inc guarantees that the original CD is free from defects in material and workmanship for one year from the date of purchase Should any defect occur you may return your CD accompanied by a dated proof of purchase Rowny Systems Inc will replace your CD free of charge ALL OTHER WARRANTIES EXPRESS OR IMPLIED INCLUDING WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE HEREBY DISCLAIMED THE DISTRIBUTOR MAKES NO WARRANTIES WITH RESPECT TO THE PROGRAM Technical Support Rowny Systems offers free technical support and advice for Antigen Plus for one year from the date of purchase Monday Thursday between 9 am and 5 pm U S Eastern Time at 301 761 3106 International callers please call 001 301 761 3106 Rowny Systems also offers
13. H Gerbich Gilfeather Xga Pr Vw Cartwright Mia Cla Jea Nyaand most Ena Chloroquine diphosphate CDP will destroy Bg and class I HLA antigens 3 If all cells in the first panel tested are reactive and the auto control is positive do a direct antiglobulin test DAT on the patient s cells before doing further serum testing 1 If the DAT is positive a warm autoantibody should be suspected and may hide underlying alloantibodies in the patient s plasma The autoantibody should be removed by autoabsorption before further antibody identification can be achieved Autoabsorption can only be performed 21 using pre transfusion patient red cells The patient must not have been pregnant or transfused in the preceding 90 days For further information and testing methods refer to the AABB Technical Manual Current Edition or the American Red Cross Immunohematology Methods And Procedures D Mallory ed 11 If the DAT is negative a cold autoantibody should be considered A cold screen tested from room temperature down to 4 C will indicate if an autologous absorption done at 4o C will be useful to remove the autoantibody Again the patient must be untransfused in the preceding 90 days 111 If autoabsorption warm or cold is not possible there are alternatives Allogeneic warm absorption is possible using RIR1 R2R2 and rr cells A prewarm method may be useful to remove any cold reactivity Prewarm methods must be used with caution prefer
14. Lot Panel Add Edit Cells ANTIGEN PLUS v7 4 0 Fie Options Donor Note Utilities Help Donor ID Lot Number Y ial Expires Supplier CHCC JC RN Location Rh Hr Ll STO N Additional Antigens Add Delete Out OF Stock G ey Group Frozen m E E E 0000 0 000 00 0 0 0 0 0 0 00 0 00000 0 0 0 0 Delete Delete Lot Clear When the Search mode is selected a search pattern box will appear that will allow you to enter a search pattern of the 28 principal antigens and or any additional antigens search the inventory for matches and mark cells for inclusion in a selected panel Search for Match gt ANTIGEN PLUS v7 4 0 DEAR File Options Donor Mote Utilities Help Donor ID Lot Number VY ial Expires Supplier HL IC H Location Rh Hr dT EO m Out OF Stock Cex Group Frozen Additional Antigens Ka A O N DOU E D eee 00000000000000000000000 0000 p SEARCH PATTERN Additional Antigens Search Additional Antigens Group 0 Frozen 0 Selected 894 Matches Found gt Search Next gt z Search Previous Select This Cell Cells Selected The search panel will immediately display how many cells are found in your database that are matches for your current search pattern and continuously displays how many cells have been selected Note that the search pattern defaults to blood group O cells The Results Panel screen can only be activated when at least one or lot have be
15. TTERR Additional Antigens Search Additional Antigens Group 0 Frozen Selected 694 Matches Found gt Search Next gt lt Search Previous Select This Cell _1 0 Cells Selected Search Pattern To enter a search pattern click the desired antigen buttons in the search pattern box They are in the same order as the labeled antigens directly above them You may set the buttons to 0 or blank A 0 or setting means that a matching cell must have a 0 or in the same place s or w designations will be read as during a search A blank setting means don t care and during a search those antigens will be ignored To search for frozen cells only click the Frozen button To search for a particular blood group click the Group button until the appropriate group designation appears To add additional antigens to the search pattern check those desired in the Additional Antigens box You may select as many additional antigens as you like but only the first 8 to 10 depending upon their length will display on the screen To remove items from the Search Additional Antigens display box uncheck the item in the Additional Antigens list 14 Searching To perform a search click the Search Next button The system will search your inventory for a cell which matches ALL the 0 and Additional Antigens in your search pattern s or w are recognized as positive during a search When you clock the Search Next button the inven
16. ably after the clinically significant antibodies have been tentatively identified Prewarm methods have been documented to remove anti H activity in a Bombay phenotype and an example of anti Vel References on request 4 If all the cells tested are negative several things must be considered 1 Repeat the cell that gave the original positive reaction that initiated the panel i e the antibody screen test or one of the units being crossmatched Note where the antibody reacts immediate spin or IAT Was the panel tested at the same phase the reaction occurred in 11 How soon after the original testing was the panel performed Was the sample stored properly 111 Is the reactive cell homozygous for any common antigen and is there another cell homozygous for the same antigen that can be used to repeat the result iv Will enhancement media help reveal a weak reacting alloantibody pattern 1 e Enz PEG LISS with a longer incubation time or polybrene v If none of this adds any information about antibody specificity you may have an antibody directed against a low frequency antigen While the identity of the antibody is a puzzle it 1s rarely a significant problem when looking for compatible units of red cells for transfusion If you have time to play with it use Antigen Plus to locate as many low incidence antigen positive cells as you have listed under special antigens If none of them react and you still wish to resolve the specificity sen
17. and may be more suitable for reading and processing by a programming language such as BASIC or C The data will be written in Donor ID Lot Number order Each cell will consist of 59 fields in the following order 1 Donor ID 2 Lot Number 3 Date 4 Location 5 Supplier 6 Vial Number 7 Rh Hr Notation 8 Blood Group 9 D Antigen 0 for negative 1 for positive blank for unknown 10 C Antigen 11 E Antigen 12 c Antigen 13 e Antigen 14 Cw Antigen Create ASCII File 38 51 58 59 f Antigen V Antigen K Antigen k Antigen Kpa Antigen Kpb Antigen Jsa Antigen Jsb Antigen Fya Antigen Fyb Antigen 25 26 27 Leb Antigen P1 Antigen M Antigen N Antigen S Antigen S Antigen Lua Antigen Lub Antigen Xga Antigen 1st Additional Antigen 56 2nd through 20th Additional Antigen Jka Antigen Jkb Antigen Lea Antigen Sex M F or blank Frozen or 1 where 1 Frozen Out of Stock 0 or 1 where 1 out of stock EJ c ROWHNY C A SYBE J AP6 Immucor Ascii PRH CW BESAPE mmucorAsci PRH EXIT CREATE Each item will occupy one field even 1f it is blank or empty If you select comma delimited any commas found imbedded in any of your data fields will be removed 29 ADDITIONAL INFORMATION COPYING TEXT FROM THE SCREEN The text in the Full description box on the Save and Load panel screens and the text in the Help screens can be co
18. at appear on the screen during the setup procedure Please note in some cases your CD drive may have a designation other than D If so use that letter instead of D in the instruction above or use the Browse command to navigate to the setup file on the CD For Network Installation Repeat the above instructions on your server or other drive accessible to the network up to the point that the setup procedure asks you to approve the default path for the AP7 directory Change the path to place the directory on the network drive on which you want the data to reside e g f ap7 There is an additional directory on the installation disc Workstation Install This is intended to install all the supporting files on individual workstations when they are to share the server AP7 program and database file The Workstation Install Setup Exe should be run from the individual workstations It will not install the executable file onto the workstation The administrator should create a shortcut to the AP7 Exe file on the workstation and have it point to the appropriate network directory If You are Upgrading from a Version Earlier Than AP7 Once you have installed the program and opened it choose the Utilities option on the top menu bar From the Utilities menu choose Import Data Click on the arrow at the right side of the SELECT TYPE OF DATA BOX and select AP2 Database or AP6 Database Using the lower box navigate to your old Antigen Plus directory usuall
19. d Cells Clear Selected Cells Load Selected Panel Save Selected Panel Rare Cell Inventory Import Data Abou Backing Up You should frequently backup your data onto a backup drive external hard drive or tape backup system The Backup Data option on the Utility menu will prompt you for the location of a directory select a directory and then click Backup to copy the file AP7 MDB to it Please note The backup utility will overwrite an Re Up calle existing backup unless you have marked the existing file as Read only In that case you must back up to H d My Book a different directory CJ Documents 4 FT Ww J Palm J Quickbooks D EXIT BACKUP Clear Selected Cells This menu option will clear all the cells selected by the Select This Cell button and there will be zero cells selected Unless the Selected Cells Panel has been saved no results will retained Add Lot to Selected Cells After a lot number has been selected clicking the mouse on Add Lot to Selected Cells will place all the cells in that lot on a Selected Cells Panel All selected cells will be checked as selected in the Search panel 23 Save Selected Panel The Save Selected Panel option is disabled unless there has been at least one cell checked or the user has loaded a saved panel To save the currently selected panel Open the Utilities dialog box and select the Save Selected Data option A new screen will appear i Save Selected Pan
20. d Rh Hr will be added to the lists in those boxes The cell display is cleared and the cursor returns to the Donor No box The Lot Number Expiration Date and Supplier are saved as a group with the Lot Number If you have changed the date or supplier of a lot then ALL cells with that lot number will have the new date and supplier A Donor Note is saved only with the Donor ID and stays with the Donor ID regardless of which Lot it is in Delete Clicking the Delete button will prompt you to make sure then remove the displayed Donor No Lot Number combination from your inventory When a cell is deleted any unique lot additional antigen or Rh Hr information it may have had will also be deleted from the lists in those boxes Delete Lot Clicking the Delete Lot button will prompt you to make sure then remove ALL cells with the displayed lot number 13 Searching and Selecting Search for Match When the Search option is selected from the File menu the Search Pattern box appears Add Edit Cells Utilities Add Change Options Help Search Print Lot Panel Exit The pattern box contains a row of 28 antigen buttons a Group button a Frozen flag button an Additional Antigens list box and the additional antigens search display box Search Next and Search Previous buttons and a check box for selecting cells a A 2 a e P B ne 0000000 0000 000000 00 00 00 0 0000 SEARCH PA
21. d on the screen at any time by selecting the Selected Cells Panel option in the File menu The Selected Panel may be printed at any time by clicking the Print button at the top of the screen Deleting a Selected Cell A cell may be removed from the selected panel preview screen by clicking anywhere in the cell s row The row will change color and you will be prompted to make sure you wish to remove the cell from the selected panel the cell will not be deleted from the system When you respond OK the cell s checkmark will be removed and the selected panel will be redisplayed from the beginning Alternatively you may locate the cell on the search screen using the Search Next or Search Previous buttons click the Select button to erase the checkmark then preview the selected panel again 17 Evaluating Test Results SELECTED PANEL TEST RESULTS Click in Results Box to PRINT HELP Indicate Test Outcome J J J J LIL LILIX I D Supplier Donor C sis klklele u u g Additional A T A c Lot Vial C w f Y ajb ajbjaj b a b a Antigens T T 1 90 9900 0 0 0 0 5 0 0 0 m x Ortho Cl 308573 RA794 Ortho Cl 114307 RA794 2 Ortho Cl 306725 RA794 3 Ortho Cl 304620 RA794 4 Ortho Cl 303854 RA794 5 Ortho Cl 308575 RA794 6 Ortho Cl 110995 RA794 7 Ortho Cl 107619 RA794 8 Ortho Cl 304952 RA794 9 Ortho Cl 306837 RA794 10 Auto Cell 0 0 0 0 0 0 0 0 0
22. d the sample to a reference lab for crosstesting Most reference labs keep their unidentified lows in hopes of finding another example 5 If your routine test method can detect weak reacting autoantibodies at any phase of testing positive antibody screen results can lead to a common panel result of several weak reactions with no specificity determined Antigen Plus will perform exclusions based on its policy and may well exclude all common specificities depending on the number of reactions and phenotype of the cells which do not react The technologist should follow his or her Blood Bank SOP for antibody identification which should address this type of panel testing outcome Depending on method used patient population and number of occurrences the most common choices are likely to include 1 Try more sensitive method if an alloantibody may be just forming or was not stimulated in the recent past 11 Repeat the original test by a less sensitive method e g from solid phase testing back to tube LISS IAT a common approach to dealing with the very sensitive capabilities of solid phase testing 111 Seen only at immediate spin assess possible clinical significance and drop the IS test if appropriate Use the prewarm method only after significant antibodies anti Vel anti PPiPx anti H in a Bombay have been excluded 22 Utilities Help Utilities ASCII File Creation Backup Data Set Supplier Antigen Order Add Lot to Selecte
23. displayed in Donor ID Lot number order If you choose In Order Checked the cells will appear in the order in which you selected them during the search If you choose Sorted by Donor Lot the cells will be displayed by Donor ID Lot number order The same order choice will govern your printed worksheet and will remain in effect until you change it from the Options menu The selected cells will be displayed 15 at a time The 28 principal antigens are displayed in the Display Option order you have selected on the search entry screen 15 Printing Antigen Plus should ensure that your printer prints in Landscape 11 x 81 2 format If your print out appears in Portrait format you may correct this in Windows by minimizing the Antigen Plus program then invoking the Start Settings and Printers commands and setting up the printer When you are finished reactivate Antigen Plus by clicking its icon at the bottom of the screen Lot Panel The Print Lot Panel option on the File menu will print all the cells in the lot displayed in the Lot Number box The cells will be in vial order and will be shown with all the data you have entered on the screen or have imported from a file see page 26 If a cell has 6 or more additional antigens and they will not all fit into the additional antigens column the last antigen will be cut short and have an ellipsis after it to indicate that there is more data than is showing The 28 principal antigens ar
24. down arrow next to the box and select the supplier name by clicking on it with the mouse Select the appropriate number for that supplier and click on the Save button Supplier Default Antigen Display Order for This Supplier mmucor a O1 02 3 04 05 O6 O7 SAVE Supplier Default Antigen Display Order for This Supplier Biotest O1 027 03 4 05 06 O7 Supplier Default Antigen Display Order for This Supplier Ortho Clinical Diag O102 03 04 58 O6 O7 Supplier Default Antigen Display Order for This Supplier Medion 0102 03 04 05 06 87 SAVE Once the supplier orders are set the screens will change automatically when Supplier Default is checked If one of the numbers is checked the screens will display the common antigens in that order only In the Supplier Default mode Selected and Results panels will print in the Supplier order of the first cell on the screen in the case of multiple suppliers Data Entry Data may be entered or changed in a box whenever the cursor is blinking in that box The cursor starts out blinking in the Donor No box and can be moved to another box by pointing to it with the mouse and clicking the left mouse button The cursor also moves to the next box by pressing the Tab key or to the previous box by pressing the Shift Tab keys Previously saved data can be viewed and selected by clicking the little down arrow to the right of the box or by pressing the Ctrl
25. e displayed in the Display Option order you have selected on the screen see pages 6 and 7 Use the Lot Panel printout to check your data against the supplier s data sheet and keep a signed copy for your validation records To verify the existence of the additional antigens whose presence is marked with an ellipsis select the cell in question in the Search mode and print it out in a Selected Panel 16 Selected Panel The Selected Panel is a worksheet containing all of the cells with check marks on them The cells are displayed in your choice of Expiration Date Checked or Donor ID Lot and vial order If a cell has been saved with an entry to the Location box the location information will be displayed instead of a lot number The 28 principal antigens are displayed in the Display Option order you have selected on the screen The worksheet will display the date and time it was printed Additional antigens are printed to the left of the principal antigens If there are more additional antigens than can print on one line more lines will be inserted underneath the cell until all additional antigens are printed These additional lines may be printed on a consecutive page if the page on which they start is filled If a selected cell has an expiration date prior to the system date the cell s first column will be marked OOD Out of Date If a selected cell is Frozen the cell s first column will be marked FZN The Selected Panel may be previewe
26. el Save Panel s New Panel lt Lookup Existing Panel Hame Humber D ate TANDELL EJ 232 956 7180 06 01 2011 Full description He Liss T andell Kaler Panel EXIT CLEAR SAVE According to your laboratory protocol enter a name and number of up to 20 characters each into the Name and Number box or lookup an existing title by clicking the Lookup Existing Panel radio button and making a selection You may add a description of unlimited length to the saved panel in the Full description text box When you are ready to save the panel with or without an extended description press Save If an existing name and number combination was selected the currently selected panel will replace that saved panel All results associated with that panel will also be saved To delete a saved panel Selecting a Name or Number will display the relevant panel and also display the DeleteThis Panel button Pressing this button will open a dialog box to ensure that you wish to delete the displayed panel DELETE SAVED PANEL 2 Delete Panel TANDELL EJ 232 56 7180 06 01 2011 cancel 24 Load Selected Data To load a saved panel Open the Utilities dialog box and select the Load Selected Data option The Load option is disabled unless there is at least one saved panel A new screen will appear i Load Selected Panel Lookup By Name Number Date Humber D ate Full description Previous antec Antre ID d Number
27. en selected All selected cells will be displayed in the Results Panel screen grid Next to each cell phenotype seven results columns are provided SELECTED PANEL TEST RESULTS Click in Results Box to PRINT HELP Indicate Test Outcome aE AAM EM E EWEA I D Supplier Donor C s s k k e e u u g Additional A JF A c Lot Vial C w f Y ajb alblalb a b a Antigens T T 0 m x Ortho Cl RA794 Ortho Cl 114307 RA794 2 Ortho Cl 306725 RA794 3 Ortho Cl 304620 RA794 4 Ortho Cl 303854 RA794 5 Ortho Cl 308575 RA794 6 Ortho Cl 110995 RA794 7 Ortho Cl 107619 RA794 8 Ortho Cl 304952 RA794 9 Ortho Cl 306837 RA794 10 0 900 0 0 0 0 5 O 0 0 0 0 0 4 0 0 0 0 0 OQ 0 0 0 0000 0 0 0 0 gt 0 0 0 0 0 0 4 S q J Rule Outs 4 3 48625 12 6 818 1114 Evaluation IAT Can Not Exclude E Kpa Lea Lua Enz Can Not Exclude E Kpa Lea Lua These are tentative rule outs Additional confirmatory data based on your own criteria are recommended P1 rule outs 4 need at least 3 negative reactions 6 found for P1 ga rule outs 3 need at least 3 negative reactions 5 found for Xga The cells displayed on the Results Panel screen will be those currently selected either in the Search mode or by using the Add Lot to Selected Cells function on the Utilities menu An autocell row will always display at the end of each Results Panel The R
28. ent order so in order to simplify entry there are 7 display options selectable with the 7 option buttons in the Options menu Click a button with the mouse pointer or Tab to the Options menu box and press the Left or Right Arrow keys to highlight a button then press Enter A The differently colored labels will move with them to make the change more obvious Note that the order inside the Rh Hr system may change the V f and Cwantigens switch places with the V label being colored differently to make this more obvious With Option 5 the order inside the MNSs group changes to SSMN The display option will be used to print Lot Panels so that the data entered can be easily compared to a supplier s antigram It will also be used to preview and print Selected Cell and Results Panels Automating the Antigen Order If you prefer to have the antigen order change on the screen to automatically match the supplier displayed on the Add Change or Search screens open the Utilities menu and select Set Supplier Antigen Order All the suppliers that have been entered into the database will display alphabetically in the Supplier list box If the Add Change and Search screens have no supplier displayed the name in the list box will be the first in the list If a supplier is being displayed on the Add Change or Search screens that supplier name will be displayed in the list box To set the default antigen order for a supplier open the list box click on the
29. esults Panel screen cannot be minimized but clicking on the Exit button at the top of the screen will not erase the current results All results will be maintained until either the Clear Selected Cells function on the Utilities menu is selected or the program is exited Simply return to the Results Panel screen through the File menu to bring back any previously entered results The entire panel and its results with comments can be saved for future reference by going to Utilities Save Selected Panel Individual cells and their associated results can be deleted by exiting the Results Panel screen and deleting the cell from the Selected Cells Panel screen Instructions for entering test results and an explanation of their interpretation may be found in this manual in the section on Evaluating Test Results Whenever a reaction is entered into a results box the columns under IAT Enz DTT and A D those antigens that are ruled out by a negative reaction will be shaded and the number of times an antigen has been ruled out is displayed on the bottom line The rule out counts for P1 and Xga are given an asterisk with an explanation in the Evaluation box below Positive P1 and Xga antigens require three negative reactions to verify their exclusion Antigen Order The antigen buttons are displayed in a common default order of blood groups Rh Hr Kell Duffy Kidd Lewis P MN Lutheran and Sex Linked Some suppliers data sheets list them in a differ
30. ing at the beginning Selecting Whenever you are in the Search for Match mode the search pattern box is present and a cell is displayed above you may click the Select box to tag this cell with a checkmark or to erase an existing checkmark You may display a cell in the search mode either by searching for it or by entering and clicking on the specific donor number in the Donor No box or by clicking on the desired number in the Donor No drop down box Cells with a checkmark are included on selected cell panels There is no limit to how many cells you may select however the preview screen can only display up to 15 cells at a time and a printed selected panel can only print up to 15 cells on a page The results screen can only display up to 11 cells at atime You may scroll to subsequent preview screens and all cells selected will print on consecutive pages To empty the Selected and Results panels without exiting the program remove ALL checkmarks by selecting the Clear Selected Cells option on the Utilities menu Previewing a Selected Panel You may preview your selected panel on the screen by selecting Selected Cells Panel from the File menu Before previewing your panel however you should choose the order in which you want them previewed and printed This is done from the Options menu If you choose Sorted by Expiration Date the cells will start with the most recent and end with the frozen cells selected The frozen cells themselves will be
31. led up again A Results panel may be printed at any time by clicking on the Print button If any cell has a long list of additional antigens obscuring the test columns the Additional Antigen column may be compressed by moving the cursor on top of the left hand border of the shaded title bar of the Additional Antigens column the cursor will change to a two headed horizontal arrow holding the mouse clicker down and dragging the border to the right The additional antigens will be hidden and the test columns will be visible without scrolling to the right of the screen An Auto Cell row will appear as the last cell of any Results panel The Auto Cell will have blank boxes in each column If typings are known for the Auto Cell they may be entered on the Results screen and will be saved and may be recalled if the Selected Cells Panel is saved Test results may be entered for the Auto Cell but those results will have no effect on the evaluation regardless of any information that is entered in the Auto Cell columns If a positive test result is entered for the Auto Cell a warning will appear in the Evaluation box a Auto Cell 0 RuleQuts 43 4825 17 8 82133 14 13242 6 IAT Can Not Exclude E WY Kpa Jsa Enz Can Not Exclude E WY Kpa dsa These are tentative rule outs Additional confirmatory data based on your own criteria are recommended Auto Cell Warning Possible autoantibody present Do DAT to determine if sensitization occu
32. mat 11 x 81 2 Validation The phenotypic information printed on a selected or results panel can be validated by assuring that its data exactly match the data from the suppliers Part of a laboratory s validation procedure should be to print each lot panel and have the responsible technologist compare it with the supplier s data sheet and sign it The signed Lot Panel sheets which have the printing date and time on them should be kept on file for as long as the cells remain on hand An ellipsis appearing on the additional antigen section of a printed lot panel indicates the presence of more additional antigens than could print on the lot panel page Those cells can be validated by selecting them with the Search mode and printing them on a Selected Panel Sheet which will print all additional antigens for that cell Antigen Plus makes no decision or final recommendation on the meaning of test results or on patient care this is the user s responsibility Antigen Plus uses the logic recommended by the American Association of Blood Banks to evaluate initial test results in the same way a technologist would do Installation For a Single User Start Microsoft Windows Place CD in your CD drive Navigate to the disk using Windows Explorer and double click on the file SETUP EXE Alternatively select the Start menu on your desktop and choose Run In the Command Line box type D SETUP EXE and press Enter Follow all instructions th
33. message will display how many cells were added 27 Rare Cell Inventory The Rare Cell Inventory Utility allows you to print out a count of all the donors with additional antigens currently in your inventory Each additional antigen will be listed once for every occurrence in the inventory with the associated donor lot and vial numbers At the end of each additional antigen list a count of unique donors with that antigen will appear You may choose individual antigens in the list box on the right or click on the All Antigens button to print a list of every occurrence of additional antigens in your inventory When you have selected the desired antigen or antigens click on the print button and the list will be sent to your default printer lt Rare Cell Inventory EXIT RAHE CELL INVENTORY REPORT In Stock Additional Antigens Add Delete Report On These Antigens E Z a Mt a Go a All Antigens PRINT 28 ASCIl File Creation Use this option on the utility menu to create an ASCII file of your cell data which can be read by other applications such as a database or spreadsheet There are two format options regular ASCII or Comma Delimited The comma delimited option separates each field with a comma has a carriage return line feed at the end of each record and is suitable for importing into most spreadsheet or database programs The regular ASCII option places a carriage return line feed at the end of each field
34. ndently Exclusions obtained from the results recorded in columns A B C and D will be exactly the same as those obtained from identical results recorded in the Liss indirect antiglobulin test TAT column There is too much variability in antibody reactivity to risk excluding an antigen on anything but the reactions obtained by the technologist and recorded in Antigen Plus Ab ID Red cell treatments when performed correctly do have a standardized effect that is well documented Antigen Plus Ab ID does employ that information when the ENZ column and DTT column have results recorded in them The ENZ column will not exclude Duffy and MNS blood group system antigens The DTT column will not exclude Kell or Lutheran blood group system antigens High frequency antigens and low incidence antigens which can be used as a search parameter to select cells for the results panel cannot be used in the exclusion process or listed in the Evaluation box at the bottom of the results panel As the antigens listed in the Additional Antigens are entered by the individual user they are not pre coded by the program A list of technical tips to assist any technologist who may have an antibody to a high frequency antigen or to a low incidence antigen is included in the Help utility and is reproduced on the following pages The following text always appears with any results in the Evaluation box These are tentative rule outs Additional confirmatory data based
35. nter one cell and save it then enter a new Donor ID number you can Tab to the Lot Number box and press the Down Arrow to recall the previous Lot Its expiration date and supplier will also appear saving you from having to re enter the data Donor Number The Donor No box will accept up to 16 characters and may be any combination of capital letters and characters except apostrophes Enter a donor ID and press the Tab key to move on to the Lot Number box If the donor is already on file the cell data will be retrieved and displayed Donor Ho 709243 Drop down or list boxes have six positioning buttons on the right hand side go to first record page up line up line down page down and go to last record One donor may be present on several different panels If a donor has more than one cell on file and her previously existing ID is entered the most recent one found will be displayed This is to facilitate entering duplicate donors when you wish to enter a donor who is already on file into a new lot number the old phenotype will automatically appear and you then need to enter only the new lot number check the expiration date and click the Save button It is assumed that the phenotype will not have changed from a previous panel Donor Note An optional note may be stored with each Donor ID by selecting Donor Note from the top menu Add Edit Cells ANTIGEN PLUS v7 0 File Options Donor Mote Utilities Help Donor ID Lo
36. pied into another application by highlighting the text by placing the mouse cursor at the beginning of the text to be copied holding down the right mouse button and dragging the cursor across the text to be copied The text will be highlighted Release the mouse button and press the Ctrl and C keys on your keyboard simultaneously The text will then be copied to the Windows Clipboard and can be copied into any Windows application that has a Paste function COPYING TEXT TO THE FULL DESCRIPTION BOX Text from the Windows clipboard may be copied into the Full description box on the Save screen When the cursor is flashing in that box press the Ctrl and V keys on your keyboard simultaneously The text you have copied from another application will be pasted into the description of the panel and may be saved with the Panel Key name by clicking the Save button on the screen PROGRAMMING NOTES AntigenPlus Ab ID is written in Visual Basic and uses a Microsoft Access database Users familiar with Access may open the databases and produce reports run queries and generally explore record layouts and file structure for purposes of importing data held in other databases Be sure to back up the original database before attempting any of these procedures 30
37. rred in wiwo or in vitro lt 19 Exclusion Policy Antigen Plus Ab ID excludes antigens in the manner described by the AABB Technical Manual 15 Edition ch 19 pg 428 9 Homozygosity is required for the exclusion in the following blood group systems Rh Duffy Kidd MNS In the presence of anti D the program will exclude E and C on heterozygous cells The blood group antigens that can be excluded on heterozygous cells are K Kpa Jsa and Lua As the homozgosity of P and Xgais not easily determined from most panel sheets the program requires 3 examples of each antigen to be non reactive with the patient sample before it will exclude either antigen It is always advisable to review AABB Technical Manual 15 Edition ch 19 pg 429 36 for more detailed information on Complex Antibody Identification including variability of antigen strength the effect of storage on RBC antigens and use of autologous cells while considering the antigens excluded or not by the program Any final decision made by the technologist must be consistent with their facility SOP for Antibody Identification The program does not encompass the knowledge of all blood group systems and their patterns of reactivity Any technologist using the program to automate the exclusion process must apply serological expertise to interpret the program s lists of antigens excluded versus antigens not excluded The program evaluates each column of reactivity indepe
38. s a working connection to the internet The first time you press Connect you will be prompted to send an email or otherwise contact Rowny Systems to set up your subscription Once the subscription is established you will see the following screen Download AntigenPlus Panels v 7 4 0 PANEL 15943 ID141 ID142 DNO 47 v DPO 46 5519 5520 RA794 17934 17938 VRC1S4 RA 93 RB316 RC381 5517 VRALS6 VRB154 VS46572 VS4573 VS454 Vo45s Connect SUPPLIER Immucor Imnmucor Immucor Immucor Immucor Ortho Clinical Ortho llinical Ortho Clinical Immucor Immucor Ortho Clinical Ortho llinical Ortho Clinical Ortho Clinical Ortho Clinical Ortho llinical Ortho Clinical Ortho Clinical Ortho Clinical Ortho Clinical Ortho llinical Load Selected Panels EXPIRES 07 08 2011 07 05 2011 07 05 2011 07 05 2011 07 05 2011 07 05 2011 07 05 2011 07 05 2011 07 01 2011 07 01 2011 06 28 2011 O6 282011 06 78 2011 06 78 2011 06 28 2011 O6 282011 06 78 2011 06 78 2011 06 28 2011 0672872011 06 28 2011 Available panels are listed in descending order of Expiration Date You may select as many as you wish and as each panel is added to your database a message shows the Lot ID and number of cells added Lot ID DPO48 1 14Cellsadded Ei If a lot already exists in your database the message will display 0 Cells Added If any of the individual cells with that lot already exist in your database the
39. s from this or previous versions of Antigen Plus Click Download From Internet to see a list of panels available online If you are importing panels that have been emailed to you select the drive and directory where they are to be found double click on the appropriate folder in the drop down list box so that the directory name appears in the Path box below and click the Load button This selection may be run any number of times without harm only those cells which are not already present will be added to your database If an existing donor in a new lot is encountered any updated information on that donor will be added as the default information for that donor You may also import entire databases from this or previous version of Antigen Plus Neither Rowny Systems nor its distributors make any guarantee as to the accuracy of any panel data supplied electronically It is the responsibility of the end user to validate the accuracy of any imported data by printing out the imported lot and validating it against the information supplied by the red cell panel manufacturer Import Data Download From Internet Panel FNL File EJ c ROWN Y JAF Download SJ Panels EJ Ortho PHL PNL 2011 355339 PNL 355341 PNL 355342 PNL 26 Download From Internet Initially you must have entered some text in menu OptionslText to appear on printed Cell Lot panels and Selected Panels You should insure that your workstation ha
40. t Humber 304034 TE Donor Hote DTT test destroys Lutheran a EXIT The note is automatically saved when you make a change A Donor Note is associated with the Donor ID only not the Lot so it will appear with every Lot that a donor ID is in When a Donor ID has a note on file a small tick mark will appear in the box Lot Number The Lot Number is used to show the supplier s unique lot number for this cell s panel All cells with a given Lot Number will have the same supplier and expiration date Lot Number The Lot Number box will accept up to 16 characters and may be any combination of numbers and capital letters Enter a lot number and press the Tab key to move on to the Vial box If the lot number was already on file the expiration date and supplier will be retrieved and displayed Vial The Vial box will accept any number from 0 through 99 It will also accept a TC tech cell vial Enter a vial number or TC and press the Tab key to move on to the Expires box Pressing the Up and Down Arrow keys or clicking the little up and down arrows to the right of the box will automatically raise or lower the displayed vial number T C is located below the 0 Expiration Date The Expires box is for the expiration date of all cells in the lot number displayed You may enter a date in any format that is comfortable for you such as 20 Dec 10 or 12 20 11 then press the Tab key to move on to the Supplier box The date
41. technical support via Email at AntigenPlus Rowny com Data Entry Support Rowny Systems offers automated red cell panels for Antigen Plus Ab ID Please contact us for more information about this service Liability Rowny Systems Inc and its distributors assume no responsibility for the use of the ANTIGEN PLUS Ab ID program The licensee assumes all responsibility for the proper use of the program including establishing appropriate protocols and validation procedures to ensure accurate data entry ANTIGEN PLUS Ab ID makes no decision or final recommendation on the meaning of test results or on patient care Contents roduc d 9220172714 E e 1 V ada O 3P22P 079 2 A E V A RRR 14 4 E EEN E E EPA 1 Wisala 11 2222 E E 2 CODE GAUION 2002207220 20 E A EE A E E 4 ADEN o S E SAE A A EEE 7 Dala Entry E E E AEE ATA E AE EA OAA 9 Searching and Selecting ccccccccecccccccccoccoccccscccccececocccccceccoccccsccccesscoocesssene 14 132 0 141 0 14 2797279072227275 E a 16 Evaluating Test IROSUNMS ssascccssestsescccssanscaccctewscssueesceessecseeessescneseesescesseoveeseeee 18 DLATE ONC E EE E O EEE E REU E E A R 1 F 20 Technical Information for Further Testing cccc00000000000000000000000000 21 8 1 01 0 2 A T AE DEE E EE R AA Teen 22 Saving Panels and ResultSs 000ccc00000000000000000000000000000000000000000000000000000000 23 Loading Panels and ResultSs ccccoooocc00000000000000000000000000000000000
42. tory will be searched starting with the NEWEST cells first that is in reverse expiration date order All frozen cells have no expiration date and will be gathered at the END of the search order Upper and lower case letters are equivalent in searching for additional antigens If you have two notations for a single antigen for example Yta and ytA a match will be found You cannot enter any new antigens into the Additional Antigens list box the list already contains all the additional antigens in your inventory A search for an unspecified blood group blank will match ANY blood type specified or unspecified If an antigen pattern or additional antigen shows 0 Matches Found and you are sure that the pattern or antigen exists it may be because the cell s blood group does not match or the cell is marked Out of Stock and the Options Exclude From Searches box is checked Each time a match is found the cell is displayed above To search for the next match click the Search Next button again A message will appear when the end of the inventory data has been reached You may also search the data in the other direction from OLDEST to NEWEST by clicking the Search Previous button a message will appear when the beginning of the inventory data has been reached Any change to the search criteria changing any search antigen button or changing the search additional antigens box will reset the search procedure and the data will be searched start
43. twork Protocols The network administrator in the interest of data security will determine each user s rights to the Antigen Plus database Antigen Plus may be configured to comply with the network rights by using the following command line parameters AP7 Exe NoSave give this group read only rights AP7 Exe UpdateSaved give this group read only rights to everything EXCEPT that the group will have rights to update and save Selected Panels Operation There are three principal modes of operation Add Change Cells Search for Match and Results Panel You can switch between the three modes by clicking the File menu with the mouse pointer or by pressing the Alt F keys to open the menu then selecting an option If no cells have been selected the Selected Cells Panel and Results Panel mode will not be available A menu option is selected by highlighting it with the mouse scrolling to the desired option with the Up and Down Arrow keys and pressing enter or by pressing a letter key matching an underlined letter in the menu option Add Edit Cells FEM Options Donor Note Utilities Add Change Search Selected Cells Panel Results Panel Exit When the program starts the Add Change screen will automatically be displayed It contains cell data boxes and buttons and includes an Additional Antigens entry box with Add and Delete buttons and Save Delete Delete Lot Clear buttons at the bottom of the screen Print
44. will be re displayed in the format set in the Windows Control Panel Regional Settings Date function If the date is before the present system date a bright red Out of Date will appear under the Expires box The expiration date is stored 10 with the lot number so if you change the expiration date of a lot number then ALL cells with that lot number will have the new expiration date Supplier The Supplier box will accept up to 20 characters You should ensure that the name is identifiable using just the first 9 or 10 characters since an abbreviated name may be printed on selected panel sheets Supplier Ortho Clinical Diag EI The supplier is stored with the lot number so if you change the supplier of a lot number then ALL cells with that lot number will have the new supplier Location Enter a storage drawer location for a frozen cell The location is stored independently and will not affect other cells with the same lot number should there be any The location will print out on the selected panel sheets in lieu of a lot number The Location box will accept up to 15 characters in any combination of letters and numbers Rh Hr Antigen Plus Ab ID comes with the standard Rh Hr phenotype designations loaded in the drop down box that is activated by clicking on the down arrow adjacent to the Rh Hr entry box Choosing one of these designations by clicking on it will place it in the entry box and will mark the appropriate pluses and
45. y c ap62 Once you click on this path a list of available files ending in mdb will appear in the SELECT FILE box Select the file you wish to import and click Load Since AP7 recognizes a cell with irregular information e g cells with Import Data Spaces in the donor or lot number or cells with missing lot or donor numbers a message will appear informing you that the cell in question is not being AP2 Database x imported Please note the donor and lot number of that cell so that you may a enter it manually after the rest of the database has imported When the ss _ Load program has completed a message confirming that you have Progam ries gt Files imported as many cells as were properly entered in your old database will appear on the screen You have now imported all your available individual cell and lot data from your prior version of Antigen Plus as well E as any panel data that was saved To Run the Program After installation 1s completed insure that YOU ARE THE ONLY USER the first time you run the program Antigen Plus must verify the structure of the database and cannot do so if there are other users This is only necessary the first time a newly installed program is run Double click the Antigen Plus icon to start the program When the program loads select the Options menu at the top of the screen Type in the name and address of your facility as you want it to appear on all the printouts generated by the program Ne
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