PlateLoc User Guide
Contents
1. H S gt _ N gt SEN ge Qu o 295 pon po U 99L 8 Feed the seal until you see the front edge of the seal material is flush with the front edge of the seal transfer plate Do not insert the seal too far or it will stick to the hot plate Ensure that the seal is centered 9 Close the seal gripper by pushing the silver gripper release button under the gripper 10 Roll any slack back up into the seal roll Be careful not to move the seal from the correct position 40 PlateLoc User Manual Chapter 5 Maintenance 11 Run at least two manual cycles using an empty plate to ensure that the seal material is feeding correctly Overall Machine Cleaning 1 Set the temperature to 30 C and wait for the machine to cool down NOTE The PlateLoc takes approximately one hour to cool down from its sealing temperature Ensure that the machine is cool before reaching inside to clean it Reaching WARNING inside the PlateLoc while it is still warm is extremely dangerous 2 Turn off power to the machine 3 Remove any scraps of seal material lying in the main sealing chamber 4 Using aclean dry cloth remove any dirt or residue from the inside of the machine 5 Using a clean dry cloth wipe down the machine exterior and the area surrounding the machine It is also safe to use alco hol or standard glass tile cleaner to clean the machine 6 Turn power back on to the machin2. NOTE Always use the thinnest insert that will sufficiently support the wells 17 PlateLoc User Manual Chapter 3 Plate Sealing Using the LCD Touch Screen Use the LCD touch screen to control the PlateLoc manually 1 Tilt the LCD screen for easier viewing e Press down on the screen release on the right side of the machine see figure 11 Pull the bottom of the screen out Allow the screen release to click into place when the screen reaches its open position Toclose press down on the screen release and push the bottom of the screen Allow the screen release to click into place when the screen reaches its closed position Figure 11 Manual Sealing Preparation L je PLA TEL yrs LCD Screen open Screen Release Door 2 Tum on power to the machine by switching the power switch to ON The Main Menu will automatically appear on the LCD touch screen see figure 12 3 Press the Setup buttton in the Main Menu to enter the Set Parameters menu see figure 12 4 TheSet Parameters menu is used to set the sealing parameters as well as to adjust the screen contrast To adjust the contrast of the LCD screen press the up or down arrow next to the symbol of the sun see figure 12 To adjust sealing parameters seal time and seal temperature press the up or down arrow on the screen next to the parameter you wish to adjust see figure 12 5 Press the Done b
3. Ifthere is no scrap the transfer plate was obstructed from reaching its transfer position at the front of the machine Remove the seal from the rear of the machine If the material is difficult to remove refer to Seal Material Feed ing Problems on page 47 e Cut off any tattered edges and reload the seal material into the machine Refer to Reloading Seal Material into a Hot Machine on page 39 e Try running the PlateLoc with a spare plate to see if the cycle completes successfully Ifthe cycle completes successfully continue with normal use of the PlateLoc Ifthe same error repeats multiple times there may be a problem with the seal material feed mechanism 48 PlateLoc User Manual Chapter 6 Troubleshooting Cycle Stopped Manually Warning The user aborted the sealing cycle by pressing the Stop button Try running the PlateLoc with a spare plate to see if the cycle completes successfully Ifthe cycle completes successfully continue with normal use of the PlateLoc Ifa new error appears find the error in the list of PlateLoc error messages Door Close Error The door was not closed for the entire seal cycle Turn the pneumatic ON OFF switch on the back of the machine to the OFF position towards the outside of the machine e Push the stage into the machine while holding the door open Gently open and close the door to ensure nothing is blocking the door or the door hinges Ifthere is noth
4. StopCycle Description Aborts the current cycle Parameters None Returns None Example Visual Basic Visual C Long PlateLocl Initialize 2 Set up on COM 2 PlateLocl StartCycle Start up a seal cycle leep 1000 Wait 1 second PlateLocl StopCycle Kill cycle nee Public Declare Sub Sleep Lib kernel32 Alias Sleep ByVal dwMilliseconds As Platel Platel Sleep Locl Initialize 2 Set up on COM 2 Locl StartCycle Start up a seal cycle 1000 Wait 1 second Platel Locl StopCycle Kill cycle 33 PlateLoc User Manual Events Chapter 4 Active X Documentation Events are fired asynchronously by the ActiveX to notify the container that a procedure has finished or an error has occurred Consult Microsoft s ActiveX documentation on how to handle events in your Visual C or Visual Basic code CycleCompleted Description This event fires whenever the sealing cycle has completed Parameters None Error Description This stock event fires whenever any fatal or non fatal PlateLoc error has occurred The SCODE parameter can be used by the container application to determine what kind of error occurred Parameters short Number BSTR FAR Description SCODE Scode LPCTSTR Source LPCTSTR HelpFile long HelpContext BOOL FAR CancelDisplay Table 6 SCodes Description SCode Communication port failed to open 3276
5. This Contract may not be modified or amended except in a writing signed by a duly authorized representative of each party Both parties acknowledge having read the terms and conditions set forth in this Contract and Exhibits attached hereto understand all terms and conditions and agree to be bound thereby 53 PlateLoc User Manual Appendix A EXHIBIT A SUPPORT SERVICES VELOCITY11 S STANDARD WARRANTY The Standard Warranty effective for one full year upon purchase date covers all parts and labor costs necessary to repair your covered product due to all functional part failures as reasonably considered under this contract Ifthe technician cannot correct the Product problem over the telephone at the initiation of a service request then the technician will follow the procedures outlined for the Support Services described in this Agreement Non shippable Products a service technician will be dispatched the next business day excluding holidays to repair the Product s Regular holidays shall include New Year s Day Memorial Day Independence Day Labor Day Thanksgiving Day the day after Thanksgiving and Christmas Day unless Velocity11 determines otherwise and notifies Customer with 30 day advance notice Shippable Products Product s must be shipped directly to Velocityll to be repaired On Site Service On site service is available for Customer Products within the United States The technician will need to have the full ad
6. PlateLoc User Manual Chapter 4 Active X Documentation Example In this example we will paint the PlateLoc bitmap over a button Visual Basic Visual C Commandl on the current form must set the Style property of Commandl to Graphical Commandl Picture PlateLocl ControlPicture Assume that there is a button named You The CPicture class will be imported into your project when the ActiveX is installed CButton button Create a button CPicture PlateLocPic PlateLocPic PlateLoc GetControlPicture Retrieve the picture button SetBitmap HBITMAP PlateLocPic GetHandle Paint the bitmap onto the button Description Initializes the COM port that the PlateLoc is attached to This method must be called before any other commands can be issued to the PlateLoc Methods Initialize Parameters short Returns None Example Visual Basic Visual C PlateLocl Initialize 2 sets the COM port to COM2 PlateLocl Initialize 2 sets the COM port to COM2 ShowDiagsDialog Description Opens a dialog box that allows you to easily test the PlateLoc From this dialog you may set the sealing time sealing temperature read the PlateLoc status codes and start a sealing cycle Parameters None Returns None Example Visual Basic Visual C PlateLocl Initialize 2 sets the COM port to COM2 PlateLocl ShowDiag
7. RUN button This button is also used to stop a cycle 7 The door will automatically close and you will hear air being released within the machine The door will automatically open and shuttle the tray outward when the cycle is complete 8 The No Plate in Holder error message will appear on the LCD indicating that no plate was found in the machine Press Clear to return to the Main Menu 9 Remove the cut piece of seal material from inside the machine 10 Repeat the cycle four more times 15 PlateLoc User Manual Chapter 2 Installation 16 PlateLoc User Manual Chapter 3 Plate Sealing Chapter 3 Plate Sealing Selecting a Plate Locator Insert Figure 9 Loading the Plate Locator and Insert 1 Place the plate locator onto the three pins of the stage 2 Place the support insert that best fits your plate type into the plate locator If the underside of the wells are flush with the bottom rim of the plate use the 80 insert If the underside of the wells are not flush with the bottom rim of the pate use an insert which will support the underside of the wells and lift the bottom rim of the plate slightly above the plate locator surface see figure 10 Figure 10 Cross Section of Plate and Support Insert Chimneys oS LA FRRRARR AKAROA I pr EEK HE PME TS TED CI Be TA LLL LL LLL TL Bu En LI LI LI LHP CC ee Ae HG IGE BEP Wells PlateLoc Support Insert 4 Plate
8. Screen does not turn on or does not respond to touches e The Touch Screen may have been disconnected or damaged on the inside of the machine Call the Velocity11 Service Center The LCD Touch Screen is dim or difficult to read Thecontrast may need adjustment To adjust the contrast of the LCD screen 1 From the Main Menu press Setup to enter the Set Parameters Menu 2 Use the up and down arrows next to the picture of the sun to adjust the contrast of the LCD Screen fthe screen remains dim or difficult to read the screen needs internal repair 45 PlateLoc User Manual Chapter 6 Troubleshooting Air Problems Insufficient Air Pressure Error Message on LCD screen e Check the pneumatic ON OFF switch on the rear of the PlateLoc Ensure that it is in the ON position towards the inside of the machine e Check the air pressure gauge on the rear of the PlateLoc It should read approximately 90 psi fit reads above 80 psi and the error message persists there is a problem with the PlateLoc s air pres sure sensor Contact the Velocity11 Service Center e Check the air supply Ensure that it is turned on and supplying enough pressure nspect the NAFD2000 air filter to see if it needs replacement For instructions on removing and changing the NAFD2000 air filter refer to Changing the NAFD2000 Filter on page 41 e Ifthe problem persists the pressure regulator within the PlateLoc may have a problem Sea
9. Support Services under this Contract Support Services shall be discontinued at the end of the then current term If Support Services are terminated for any reason at Velocityll s sole option Customer may be permitted to reinstate or renew Support Services by paying Velocity11 applicable Support Services fees LIMITED WARRANTY Velocityll warrants that Support Services will be performed with the same degree of skill and professionalism as is demonstrated by like professionals performing services of a similar nature LIMITATION OF LIABILITY Direct Damages VELOCITY 11 S SOLE LIABILITY AND CUSTOMER S EXCLUSIVE REMEDY FOR DAMAGES WITH RESPECT TO THE SUPPORT SERVICES UNDER ANY CONTRACT TORT INCLUDING NEGLIGENCE STRICT LIABILITY OR OTHER THEORY SHALL BE LIMITED TO THE AMOUNT PAID BY CUSTOMER FOR THE SUPPORT SERVICES Consequential Damages UNDER NO CIRCUMSTANCES INCLUDING NEGLIGENCE SHALL VELOCITY11 BE LIABLE FOR ANY SPECIAL INCIDENTAL OR CONSEQUENTIAL DAMAGES INCLUDING WITHOUT LIMITATION DAMAGES FOR LOST PROFITS LOSS OF DATA OR COSTS OF PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES ARISING IN ANY WAY OUT OF THIS CONTRACT OR THE USE OF THE LICENSED PRODUCT DESIGN TECHNIQUES AND DOCUMENTATION ENTIRE CONTRACT This Contract and the attached Exhibit constitute the entire Contract between the parties regarding Support Services and supersede all previous Contracts or representations oral or written regarding the subject matter
10. roll with hubs and axle onto the seal roll supports The roll should be placed so that the seal feeds from the underside of the seal roll as opposed to feeding from the top of the roll The roll should fit easily into the two seal roll sup ports 5 Feed the seal from the front of the roll toward the back along the top of the machine and down the back Be sure to feed the seal under the bar connecting the roll supports 6 Feed the material into the seal roll slot directly above the lower seal gripper If you have problems loading the seal ensure that the edge entering the machine has not curled NOTE down Curl the edge up by hand to remove any downward curl 7 Figure 15 shows the path that the seal material takes through the machine Look into the front opening of the machine You should see the seal material emerging from on top of the seal transfer plates see figure 15 The surface above the main chamber is extremely hot Do not insert hand into the VAN WARNING door of the machine Adjust the position of the seal by moving it at the back of the machine Figure 15 Feeding Seal Material into a Hot PlateLoc Seal Material o Feed seal material into the Transfer Plate PlateLoc until the seal material s flush with the edge of the transfer plate L
11. the user tries to set a temperature that is too low the temperature is automatically set to 20 C Similarly if the user tries to set a temperature that is too high the temperature is automatically set to 230 C In either case an error event is fired short None Visual Basic Dim temp As Short temp 196 PlateLocl Initialize 2 F Visual C PlateLocl Initialize 2 sets the COM port to COM2 sets the COM port to COM2 short itemp itemp 196 PlateLocl DesiredT mperature temp PlateLocl SetDesiredTemperature itemp 24 PlateLoc User Manual short CurrentTemperature Chapter 4 Active X Documentation CurrentTemperature is the present temperature in Celsius of the hot plate GetCurrentTemperature Description Retrieves the current temperature in Celsius of the PlateLoc s hot plate If an error occurs GetCurrentTemperature will return 1 and an error event will be fired Parameters None Returns short Example Visual Basic Visual C PlateLocl Initialize 2 sets the COM port to COM2 Dim temp As Short temp PlateLocl CurrentTemperatur PlateLocl Initialize 2 sets the COM port to COM2 short iTemp iTemp PlateLocl GetCurrentTemperatur SetCurrentTemperature Not implemented double SealingTime SealingTime refers to the length of time needed to seal a microplate in seconds GetSealingTi
12. thirty 30 days prior to the expiration date of the Product Warranty or Support Services contract and will have the option of purchasing additional coverage based on the current Support Services rates at that time Velocity11 may suspend or terminate Support Services if Customer fails to timely pay Support Service fees as provided in this Contract Velocity11 may also terminate Support Services if Customer breaches any provision of this Contract and such breach is not remedied within thirty 30 days after Customer receives written notice of the breach Velocityll shall also have the right not to renew this Contract with respect to any Product by providing written notice of such election at least ninety 90 days prior to the termination of Support Services for such Product provided that Velocity 1 no longer generally provides Support Services for such Licensed Product or no longer provides the specific Support Services previously offered Customer may terminate this Contract if Velocity11 materially breaches its obligations under this Contract If Customer terminates this Contract due to a material breach on the part of Velocity11 Velocityll will refund the support fees for the then current term paid by Customer under the Contract from the date of termination prorated to the end of the then current term 52 PlateLoc User Manual Appendix A 8 1 8 2 REINSTATEMENT OR RENEWAL OF SUPPORT SERVICES In the event Customer fails to timely renew
13. 7 Time is invalid 32766 Temperature is invalid 32765 Could not create status thread 32764 Could not create temperature thread 32763 Communication port is not open 32762 Waiting for status 32761 Waiting for temperature 32760 Could not create cycle thread 32759 Cycle error 32758 No dialog thread 32757 Waiting for current sealing time 32756 Waiting for desired temperature 32755 Cycle never started 32754 No response to cycle start command 32753 Cycle start command not acknowledged 32752 No response to Set t ime command 32751 34 PlateLoc User Manual Table 6 SCodes Chapter 4 Active X Documentation Description SCode Settime not acknowledged 32750 Error with Sett ime command 32749 No response to Sett ime command 32748 Settemp command not acknowledged 32747 Error with Sett emp command No response to Clearerror command 32746 32745 Clearerror command was not acknowledged 32744 Error with Clearerror command 32743 Cycle did not finish Non runtime error 32742 32741 No response 32740 Error with Abortcycle command Abortcycle command not acknowledged No response to Abort cycle command 32739 32738 32737 35 PlateLoc User Manual Chapter 4 Active X Documentation 36 PlateLoc User Manual Chapter 5 Maintenance Chapter 5 Maintenance The PlateLoc needs little regular maintenance to keep it running smoothly All repairs
14. Aer PlateLoc User Manual version 2 1 435 Acacia Avenue Palo Alto CA 94306 650 846 6500 www velocity11 com Information in this document is subject to change without notice copyright 2001 by Velocity11 Reproduction in any manner whatsoever without the written permission of Velocity11 is strictly forbidden Microsoft and Windows are registered trademarks of Microsoft Corporation Other trademarks and trade names may be used in this document to refer to either the entities claiming the marks and names or their products Velocity11 disclaims any proprietary interest in trademarks and trade names other than its own Rev 2 1 October 2001 PlateLoc User Manual Table of Contents Table of Contents Chapter I General Information cocieciccsesccccctecccsvsseescetsscessecevssceosseesdscssascece vocessouesvetensevectesvasccavevecescteseassenostes 5 Introduction 4 45 n E ee t DR DI a DERE ee inate ea aiio s 5 System Requirements 55 sg ee dete p deti eate dr dede e iR eee 5 Warranty Information and Service Contracts sse 6 Warnings and Advisories 1 1 na te E prede ise E 6 Chapter 2 Installation 5 reet esse nero INE ne Nene Vou e NEUMS engen EN Spee ue ea SSO S ESES 7 Unpacking the PlateLocIM sanie e IH e canes E e e anne an 7 IUE P PE 8 Setting up the PlateLoc noceant e e e ette eder sd e e edes 11 Cold Running the PlateLO6 niet dp et an OO rise eine 14 Chapter 3 Plate Sealing ssor rensz
15. Check that the seal roll sits straight within the seal roll supports Ifthe problem persists the seal roll may be damaged If a spare seal roll is available check to see if the same problem exists with the spare roll Seal material does not feed into the seal roll slot e Check that the pneumatic ON OFF switch on the PlateLoc is switched to the ON position towards the inside of the machine e Check that the seal gripper is lowered by pressing the silver gripper release button and then pushing down on the lower seal gripper until it clicks Turn the power off and on again The machine powers on ready to accept seal material e Check that the front edge of the seal material has not curled down or wrinkled Cut off any curled or wrinkled seal and try to reinsert the material into the seal roll slot Look through the seal roll slot from the rear of the machine You should be able to see through to the front open ing of the machine If the view is obstructed there may be scraps of seal material obstructing the seal material path 47 PlateLoc User Manual Chapter 6 Troubleshooting PlateLoc Error Messages Hot Plate Vacuum Error The hot plate did not detect that a full piece of seal material was ready to be applied to the plate Look inside the machine to see if there is a scrap of seal material If there is a scrap of material in the machine remove it e Try running the PlateLoc with a spare plate to see if the cycle com
16. Loc User Manual Chapter 6 Troubleshooting Seal Material Feeding Problems Seal material is difficult to remove from the machine e Turn the power off The transfer plates will automatically release the seal material e Ifthe seal material is still difficult to remove the seal may be stuck inside the PlateLoc e Turn the pneumatic ON OFF switch to the OFF position towards the outside of the machine This will release the internal mechanisms from their fixed positions Ifthe seal material is NOT piercable seal try to pull the seal material out using significant force if nec essary If using piercable seal try to pull the seal material out being careful not to tear the seal e Ifthe seal material remains stuck or tears contact the Velocityll Service Center to have it removed internally Seal material does not feed straight e Check that the seal roll hubs are screwed tightly against the seal roll e Check that the seal roll sits straight and is seated fully within the seal roll supports e Check that the seal material is being fed underneath the bar connecting the two seal roll supports e Check that the seal gripper is closed by pressing the silver gripper release button Ifthe seal feeding does not improve after 10 to 20 cycles check the cutter blade Refer to Inspecting the Cutter on page 43 Seal roll does not spin easily in roll supports e Check that the seal roll hubs are screwed tightly against the seal roll e
17. OFF Switch re a 3 ID ON C AE en Jorr gt EI AN O CoH Power Switch 01 ON Ol OFF Air Input Port Power Port 120VAC Q 85 29 Serial Communications Port 3 Turn off the air supply and bleed off any excess air remaining in the line between the air compressor and the PlateLoc 4 Hold the filter body and unscrew the filter bowl see figure 17 Figure 17 Unscrewing the NAFD2000 Filter Bowl Filter Body 5 Check if the filter is saturated A saturated filter is the final indication that the filter needs to be replaced 6 Unscrew the dirty filter from the filter body and replace it with a new one see figure 18 Figure 18 Unscrewing the NAFD2000 Dirty Filter Filter Screw the filter bowl back on the filter body Turn on the air supply and ensure that it reads the proper pressure 7 Switch the pneumatic ON OFF swtich on the back of the PlateLoc to the ON position Ensure that the pressure gauge on 8 9 the back of the machine reads about 90 psi When you turn on the air supply to the PlateLoc the door will open and the stage A N WARNING will extend Keep the area in front of the PlateLoc clear 42 PlateLoc User Manual Chapter 5 Maintenance Inspecting the Cutter 1 Set the temperature to 30 C and wait for the machine to cool down NOTE The PlateLoc takes approximately one hour to cool down from its sealing temperature Ensure that the machine is c
18. Troubleshooting Chapter 6 Troubleshooting The following troubleshooting guide offers a series of steps to follow to correct problems with your PlateLoc as quickly as possible If problems persist or if you have any questions regarding the troubleshooting procedure please contact the Velocity11 Service Center covers Refer servicing to qualified Velocity11 personnel only To avoid electrical shock and possible severe injury do not remove PlateLoc WARNING The Velocityll Service Center is always available to assist you with any of your PlateLoc needs Please feel free to contact the Velocity11 Service Center at any point in the troubleshooting process For information regarding your PlateLoc service plans consult Velocityll s Product Warranty on page 51 NOTE Velocity11 s Service Center can be reached at 650 846 6500 Power and LCD Touch Screen Problems The PlateLoc does not turn on e Check the power cable on the back of the machine to ensure that both ends are plugged in correctly e Check the power switch to ensure it is switched to the ON position toward the inside of the machine Looking through the front door of the machine check if there is a red light turned on within the main chamber of the machine Ifthe red light is on there is a problem with the LCD screen Refer to the next section on checking the LCD screen Ifthe red light is NOT on there is a problem with the PlateLoc s power supply The LCD
19. alize 2 PlateLocl Initialize 2 Dim iErrorFlags As Short short iErrorFlags iErrorFlags PlateLocl ErrorFlags iErrorFlags PlateLocl GetErrorFlags SetErrorFlags Not implemented Table 4 Flag Bit Protocol Definitions Bit Definition Bit 0 Set Cycle Finished Clear Cycle Not Finished Bit 1 Set Fatal Error Bit 2 Set Non Fatal Error Bit 3 Set Insufficient Air Vacuum Error Bit4 Set Sensor Error Bit 5 8 Spare VARIANT ErrorString ErrorString contains a text explanation of the last error asserted by the PlateLoc A summary of the error strings is given in table 5 GetErrorString Description Retrieves the PlateLoc s error strings Parameters None Returns VARIANT Example Visual Basic Visual C PlateLocl Initialize 2 PlateLocl Initialize 2 sets the COM port to COM2 sets the COM port to COM2 Dim ErrorString As String VARIANT vErrorString ErrorString PlateLocl ErrorString vErrorString PlateLocl GetErrorString CString sStatus sStatus vErrorString bstrVal 28 PlateLoc User Manual Chapter 4 Active X Documentation SetErrorString Not implemented Table 5 Error Strings Error String Flag Bits Set Transfer Plate Vacuum Error 2 3 Keystone Error 2 Low Air Pressure Error 2 3 Gripper Plate Vacuum Error 2 3 Hot Plate Vacuum Error 2 3 Overheat Error 2 Cycle Stoppe
20. ate sealing or integration into a larger automation platform this user s manual will walk you through the required steps to get your unit working to its fullest capacity Velocity 11 strives to produce the highest throughput and highest quality products available for the laboratory automation market Technical information on the PlateLoc and other exciting Velocityll products are available from Velocityll With questions or comments please contact our Service Center at 650 846 6500 or visit our website at http www velocity11 com System Requirements Stand Alone Unit 8 5 x 13 5 benchspace 1 4 compressed air line capable of supplying 2 5 cfm at 90 psi with 0 05 um filtration e 120 VAC 50 60 Hz 5 amps factory installed 220 VAC models are also available Automated Platform Same as the stand alone unit above Pentium 166 or better PC Windows NT4 Service Pack 6 or Windows 2000 e 32MBRAM e Serial I O RS 232 9 pin connector PlateLoc User Manual Chapter 1 General Information Warranty Information and Service Contracts All Velocity11 products are covered under warranty for parts and labor for the first year of service see table 1 Table 1 Velocity11 Standard Service Warranty Shippable Items The damaged unit will be shipped to Velocityll for repair Upon repair the unit will be shipped back to you Non Shippable A technician will be dispatched to your site the next Items business day not including holiday
21. cts other than the current version of the product unless otherwise covered under an existing service contract or agreement c any Product with software that has been modified or is out of date without the written approval and authorization of Velocity 1 d Product problems caused by Customer s negligence Customer abuse or misapplication including but not limited to use of incorrect voltages use of incorrect fuses use of incompatible devices or accessories and improper or insufficient ventilation use of Product other than as specified in the user manual or other causes beyond the reasonable control of Velocity11 including but not limited to an act of God such as lightning floods tornado or earthquake e Products installed on any hardware operating Product version or network environment that is not supported by Velocity 1 f Products sold or transferred by the Customer to another entity FEE SCHEDULE Customer shall pay the annual fees for Support Services for the initial term of this Contract within thirty 30 days of the Effective Date Thereafter unless Support Services are not renewed Customer shall pay annual Support Services within 30 days of the commencement of the then current term for such Support Services TERM AND TERMINATION The initial term of this Contract is one 1 year from the Effective Date of this Contract unless earlier terminated in accordance with this Contract The Customer will be notified within
22. d Manually 2 No Plate in Holder 2 4 Temperature Sensor Error 2 4 Memory Access Error 1 Actuator Timeout Error 2 Serial Communications Error 2 Door Open During Cycle 2 4 Insufficient Vacuum Pressure 253 long ProcessTimeout ProcessTimeout represents the allowable length of a sealing cycle in mS If the cycle exceeds this length an error is asserted The default timeout is 10000 ms 10 seconds 29 PlateLoc User Manual Chapter 4 Active X Documentation GetProcessTimeout Description Returns the current timeout in ms Parameters None Returns long Example Visual Basic Visual C PlateLocl Initialize 2 PlateLocl Initialize 2 sets the COM port to COM2 sets the COM port to COM2 Dim iProcessTimeout As Long long iProcessTimeout iProcessTimeout iProcessTimeout PlateLocl ProcessTimeout PlateLocl GetProcessTimeout SetProcessTimeout Description Sets a new process length in ms Parameters long Returns None Example Visual Basic Visual C PlateLocl Initialize 2 PlateLocl Initialize 2 sets the COM port to COM2 sets the COM port to COM2 PlateLocl ProcessTimeout 10000 PlateLocl SetProcessTimeout 10000 Set the process timeout to 10 seconds Set the process timeout to 10 seconds ControlPicture Description Retrieves a picture of the PlateLoc bitmap that can be used in the container s application Parameters None Returns IPictureDisp 30
23. ded Ensure that the serial command input is correct e fthe error persists contact the Velocityll Service Center 50 PlateLoc User Manual Appendix A Appendix A Velocity11 s Product Warranty Velocityll a California corporation with its principal place of business at 435 Acacia St Palo Alto California 94306 Velocity11 is the obligor of this service contract Contract This Contract sets forth the terms and conditions under which Velocityll will provide Support Services as defined below for its Products covered under the Product Warranty guidelines 1 1 1 3 1 3 2 DEFINITIONS Support Services means the support and or services that are available from Velocity 1 as further described in Exhibit A may be amended from time to time COVERAGE Velocityll will provide the Support Services set forth in Exhibit A and pursuant to the terms and conditions of this Contract for one year after the purchase date of the product Additionally the Customer has the option to purchase an enhanced Platinum Support Services contract Terms conditions and rates of enhanced coverage are set forth in Exhibit B of this Contract Within 30 days prior to the expiration date of the Product Warranty or Support Services contract Velocityll will offer the Customer the option of purchasing additional coverage based on the current Support Services rates at that time SUPPORT SERVICES In order for Customer to r
24. dress of Customer Products location A service technician will be dispatched to Customer s location to service Customer s Product The Services provided under this Agreement are repair services that are necessary because of any defect that exists or occurs in materials or workmanship in the Products or in any Product component covered by this Agreement The Standard Warranty does not include preventive maintenance installation deinstallation relocation services or operating supplies If Customer or Customer s authorized representative are not at the location when the service technician arrives Velocityll will no longer be obligated to provide services under this Agreement for the Product that requires service on such visit The service technician will leave a notice with the time of arrival as proof of arrival Consequently you may be charged an additional fee for a second service call to repair your instruments Service Area This Agreement applies only to Products located in the United States In addition Velocity11 may invoice Customer an additional charge for service in certain remote areas of the United States Velocityll will notify Customer if Customer Products are in an area where Velocity11 cannot offer on site service If Customer relocates its Product s to a geographic location in which the Standard Warranty service is not available Customer may incur an additional charge to maintain the same categories of service coverage at the new
25. e and reset the machine to its sealing temperature Changing the NAFD2000 Filter The frequency with which the NAFD2000 filter needs to be changed depends on the particular air supply setup and overall PlateLoc usage An Insufficient Air Pressure Error is the initial signal that the filter may need to be changed Upon receiving an Insufficient Air Pressure Error check the following conditions to determine if your filter needs to be changed The PlateLoc gives an Insufficient Air Pressure Error when trying to run The pressure gauge on the rear of the PlateLoc reads less than 90 psi The pressure gauge at the air compressor reads 90 psi or greater If all the above conditions are met the NAFD2000 filter may need to be changed To verify that the problem is a dirty filter remove the filter from the air line running to the PlateLoc If the pressure on the back of the PlateLoc reads correctly the filter needs to be replaced If the pressure still reads incorrectly call the Velocityl1 Service Center The following steps describe how to change the NAFD2000 filter 1 Turn off the PlateLoc 2 Switch the pneumatic ON OFF switch on the rear of the PlateLoc to the OFF position toward the outside of the machine see figure 16 41 PlateLoc User Manual Chapter 5 Maintenance Figure 16 PlateLoc Control Panel Pneumatic Gauge C Io 7 FE Pneumatic ON
26. eceive the Support Services described in Exhibit A Customer must a Initiate a service request by contacting Velocity 11 at 650 846 6500 b Provide the serial number of the Product a description of the problem and the current version of the control software c Provide a detailed description of the type of error a description the specific error message and when it occurs the activity or use of the Product when the error occurred and remedial measures taken by the Customer to correct the error d Follow procedures and recommendations provided by the Velocity11 technician in an effort to correct problems via telephone VELOCITY11 WILL NOT DISPATCH A SERVICE TECHNICIAN TO PERFORM ON SITE REPAIRS UNTIL THE TELEPHONE BASED TROUBLESHOOTING PROCESS HAS BEEN EXHAUSTED e Subject to Customer s applicable security requirements provide Velocity11 with access to and use of all information and Product facilities including working space electricity and a local telephone line necessary for Velocityll to provide timely Support Services pursuant to this Contract f To the best of its abilities read comprehend and follow operating instructions and procedures as specified in but not limited to Velocityll documentation and other correspondence related to the products Velocityll shall have no obligation to support 51 PlateLoc User Manual Appendix A 5 1 5 2 5 3 a altered or damaged Products b any version of the Produ
27. eerte 43 Lubricating the Door Hinges x eese gie eee de aan nmel 43 PlateLoc User Manual Table of Contents terrae Eripuit Power and LCD Touch Screen Problems Air Problems snin PEE Sealine Problems u nern este Perse eere etre s iere e Ye eR ER EY Seal Material Feeding Problems PlateLoc Error Messages Appendix A Velocityll s Product Warranty PlateLoc User Manual Chapter 1 General Information Chapter 1 General Information Introduction Thank you for purchasing a Velocityll PlateLoc thermal plate sealer The PlateLoc is a self contained module for applying thermal seals to microplates These seals prevent contamination and evaporation of valuable samples during transport or storage The PlateLoc consistently achieves a high quality seal with its sophisticated thermal control system The PlateLoc needs no adjustment to seal plates of various heights The PlateLoc s small footprint allows easy robotic integration and its touch screen display enhances usability in manual applications Above all the PlateLoc s processing time and operating costs are significantly less than competing products making it the optimal solution for both manual and automated sealing applications The purpose of this guide is to provide the basic information necessary to achieve the optimal performance from your PlateLoc Whether your application is manual pl
28. er et eerte eee ehe Suae NUES SPUREN ee PEN RUeTe NEN nS EN eR a E Evo nes 17 Selecting a Plate Locator Insert necis e o RE ER E TR Edge 17 Using the LCD Touch Screen euet date e ERU OK 18 Setting Seal Time and Temperature ccccceccceseceseeseeseesseesceeseceecesecseeeseceseeseeeeeseeeeeesseeeaeeaeeseenseeseeas 19 Manually Sealing a Microplate sss eene eene nnne nennen nnn 20 Chapter 4 Active X Documentation rine ea erret reto rientro caves ne aee esee Sepe ERES NUR eS NER Yee Ene rea sence deneseases 23 Installing PlateLoc Software aan t e e E ERE Te VERE CERE ERES 23 Properties ce eee o ten Pea a n cid Re T RI RE IS Re ERE 24 State Codes as e eodein eoe este tr i preesse ia tab ue eec ee aes 27 Methods es dp et ee tte ter tide et E eas eins 31 EV6entS incusat eto en ser eurer Hrn HERE Om 34 Chapter 5 Maintenance 220 05 scccescsccctsesecetstececesedacscstespnescsssveesevssetocsensvsocssunscoveeseoessenessontcensoeestcosestestedessaecesseee 37 Generali Maintenance Schedule 8 3 2 tm e RE ee tei teet de perte um 37 Cleaning the Hot Plate c tere ee rti e hee aee E e In e e ee ee er ts 38 Changing the Seal Roll tane tede oed PE er eiue 38 Reloading Seal Material into a Hot Machine essere eere nenn 39 Overall Machine Cleanmg tai oen eo ee e i b E ed tede 41 Changing the NAFD2000 Filter 4 d aneidai ehe RR eis 41 Inspecting the Cutter ccs rs tese tete date ta e s ce apt
29. five times at a cold temperature and without a plate This ensures that the PlateLoc is functioning properly and the seal material is feeding correctly 1 Turn on power to the PlateLoc by switching the power switch at the rear of the machine to the ON position The LCD touch screen will turn on and display the Main Menu as shown in figure 7 Figure 7 PlateLoc Main Menu Main Menu Seal Time 0 5 S Seal Temp 30 C Status Warming Up Setup ty VELOCITYII 2 Press Setup on the Main Menu This will take you to the Set Parameters Menu see figure 8 To adjust the contrast ofthe LCD screen press the up or down arrow next to the symbol of the sun Figure 8 PlateLoc Set Parameters Menu Set Parameters Menu Set Seal Time 0 5 S Set Seal Temp 30 C va 3 Ensure that the temperature is set to 30 C and time is set to 0 5 s Use the up or down arrows next to the corresponding paramter to adjust the value 4 Press Done to return to the Main Menu 14 PlateLoc User Manual Chapter 2 Installation 5 The PlateLoc may take a few minutes to reach the temperature setting The Status display in the Main Menu will indicate 29 66 whether the Machine is Warming Up Cooling Down or Ready NOTE The RUN button remains blank until the temperature reaches the required level 6 When the RUN button appears press RUN to execute a cold cycle test of the PlateLoc NOTE Do not hold your finger on the
30. he line has been properly installed PlateLoc User Manual Chapter 2 Installation 8 Turn on the air supply Listen for leaks in the air line connections 9 Turn the pneumatic ON OFF switch for the PlateLoc to the ON position toward the inside of the machine see figure 1 When you turn the air supply on the door will open and the stage will extend When you turn the air supply on the door will open and the stage will extend WARNING Keep the area in front of the PlateLoc clear 10 Check the pneumatic gauge on the rear of the PlateLoc to ensure it reads approximately 90 psi Install the Power Cable Operating the PlateLoc at the wrong voltage could seriously damage the CAUTION instrument Before turning on the system ensure that the voltage supplied to the PlateLoc system is 120 VAC 50 60 Hz and the line is rated to supply a minimum of 5 amps Also ensure that the red box directly above the power switch reads 120V If it does not contact your Velocityll service representative 220 VAC models of the PlateLoc are available but must be factory installed Contact Velocity LI for more details 1 Turn the power switch on the back of the PlateLoc to OFF position see figure 1 2 Plug one end of the power cable into the 120 VAC power port on the rear of the PlateLoc see figure 1 3 Plug the other end of the power cable into a grounded electrical socket that hair clothing and jewelry do not become caught in the
31. hold your finger on the RUN button This button is also used to stop a cycle 6 The plate will be shuttled into the machine The shuttle door will close automatically You will hear compressed air being released and the pneumatic cylinders moving as the plate is processed 7 The shuttle door will open and the sealed plate will be shuttled out of the machine Note that the seal on the plate will be warm to the touch 20 PlateLoc User Manual Chapter 3 Plate Sealing The inside of the PlateLoc remains hot for approximately one hour after WARNING being powered down Checking Seal Qualit To determine if the plate has been properly sealed 1 Manually seal a plate 2 After the plate has been sealed wait five seconds for the plate and seal to cool down 3 Remove the plate from the locator and carefully peel off the seal material from the plate 4 Inspect the underside of the removed seal material A plate that has been properly sealed will leave unbroken impressions of each chimney on the underside of the seal material If there are faint or broken impressions on the seal material the plate may need to be sealed for a longer time or at a higher temperature Visually inspect the plate If the top of the plate looks overly melted the plate may need to be sealed for a shorter time or at a lower temperature 21 PlateLoc User Manual Chapter 3 Plate Sealing 22 PlateLoc User Manual Chapter 4 Active X Documentati
32. ing Seal Material into a Hot Machine on page 39 38 PlateLoc User Manual Chapter 5 Maintenance Removing an Empty Seal Roll 1 Detach any leftover seal material from the existing seal roll 2 Remove the seal roll and hubs from the seal roll supports see figure 14 Figure 14 Removing the Empty Seal Roll 7 Empty Seal Roll SAN 0 b A ARIA Seal Roll Supports 3 Unscrew one hub from the axle and remove the empty seal roll 4 To remove the leftover seal material open the seal gripper by pressing down on the lower seal gripper Pull out the left over seal material from the back of the machine The Hot Plate is still EXTREMELY HOT Do not insert hands through the front AN WARNING 4 door of the machine Reloading Seal Material into a Hot Machine The following procedure describes how to change the seal roll while the Hot Plate AN WARNING is still hot Follow this procedure only when necessary Use extreme caution when interacting with a hot PlateLoc 1 Place the new seal roll onto the axle The roll should fit snugly against the seal hub 2 Screw the second hub back onto the axle Tighten until the hubs are securely holding the roll 3 Using scissors make a clean straight cut across the end of the seal material cutting off any part of the seal that is wrinkled or torn The PlateLoc will not work with wrinkled seal 39 PlateLoc User Manual Chapter 5 Maintenance 4 Place the seal
33. ing is a complete list of the contents of the Velocity11 PlateLoc packages e PlateLoc e Seal material e Power cable e Serial communications cable e 15 feet of 1 4 polyurethane tubing Three hose fittings optional depending on your house air configuration 1 4 quick connect 1 4 NPT 1 4 quick connect 1 8 NPT 1 4 quick connect T e NAFD2000 0 01 um air filter NAFD2000 Filter Installation Instructions e Seal roll axle amp hubs e Plate locator Support inserts PlateLoc User Manual PlateLoc ActiveX CD Inspection Check that all items from the list above have been included in the PlateLoc packages Inspect all items for possible shipping damage before continuing the installation process If anything is missing or appears damaged contact a Velocityll service representative as soon as possible NOTE Save the carton and packing materials in case you ever need to ship the unit PlateLoc User Manual Chapter 2 Installation Installation Figure 1 Control Panel Air and Power Connections Pneumatic Gauge Pneumatic ON OFF Switch a Power Switch ON O OFF i a t VUE Power Port y eLA Serial Communications Port e SE Install the Compressed Air Line Working with open charged air lines can result in injury Proper procedure N WARNING should be used to bring an air line int
34. ing obstructing the door but it will not close fully call the Velocity11 Service Center Ifthe door closes fully and the error message persists the door sensor may be malfunctioning If the door closes fully turn the pneumatic ON OFF switch to the ON position and the PlateLoc NOTE stage will push the door open No Plate in Holder Error e Check that the plate was positioned properly in the plate locator e Run the PlateLoc again If the error repeats there may be a problem with the plate sensor Temperature Sensor Deviation Error The PlateLoc encountered a problem reading the temperature of the hot plate The heaters automatically turned off and the machine will start to cool down e Restart the PlateLoc e Ifthe error persists call the Velocity11 Service Center Overheat Error The PlateLoc s temperature control system has malfunctioned Monitor the temperature of the machine displayed on the LCD screen If the temperature continues to rise power down the machine and unplug the machine from the outlet e Contact the Velocity11 Service Center Keystone Error or Memory Access Error PlateLoc encountered problems accessing system memory e The PlateLoc memory may have been corrupted Call the Velocity11 Service Center to verify that the PlateLoc settings are still correct 49 PlateLoc User Manual Chapter 6 Troubleshooting Serial Communications Error The serial communications input buffer has been overloa
35. l that is wrinkled or torn The PlateLoc will not work with wrinkled seal 6 Place the seal roll with hubs and axle onto the seal roll supports The roll should be placed so that the seal feeds from the underside of the seal roll as opposed to feeding from the top of the roll The roll fits tightly between the two seal roll sup ports see figure 4 11 PlateLoc User Manual Chapter 2 Installation Figure 4 Proper Seal Mounting and Loading E Ex X Y PS IN M MEETS L1 ILI 2 Lm 72 Seal Roll Supports Loading the Seal After the seal roll is mounted the seal itself must be loaded into the machine 1 Feed the seal from the front of the roll toward the back along the top of the machine and down the back Be sure to feed the seal under the bar connecting the roll supports see figure 4 Figure 5 Control Panel Seal Gripper Detailed View Seal Roll Slot e O71 c ane gt SC Lower Seal Gripper Gripper Release Button EN NC pp VA f i e LL m 12 PlateLoc User Manual Chapter 2 Installation 2 3 4 5 6 7 8 The following procedure for loading the seal roll should ONLY be followed when the machine is cold room temperature For instructions on reloading the seal roll while the machine is hot refer to Reloadi
36. ling Problems The plate sticks to the hot plate There may be residue on the hot plate Refer to Cleaning the Hot Plate on page 38 The seal material does not cut e The cutter blade may need to be sharpened or adjusted Refer to Inspecting the Cutter on page 43 to determine the problem with the cutter The seal edge is tattered or cut unevenly e The cutter blade may need to be sharpened or readjusted Refer to Inspecting the Cutter on page 47 to deter mine the problem with the cutter The plate does not seal completely or seals unevenly e Check that you are using the correct support insert See Selecting a Plate Locator Insert on page 17 to deter mine the proper insert for your plate e Refer to the standard seal times and temperatures listed in Table 2 and ensure your setup corresponds to the table e Inthe Set Parameters menu increase the seal time Different plates demand different seal times and temperatures than those suggested by the seal manufacturer Inspect the top surface of the plate to ensure it is flat Warped or crooked plates may seal poorly e Ifthe plate continues to seal poorly examine the plate carefully Velocity11 s PlateLoc is specifically designed to seal microplates whose well edges chimneys are raised above the surface of the plate Plates with chimneys lower than the surface of the plate may not seal well or may require a longer seal time and higher temperature 46 Plate
37. location If Customer chooses not to pay such additional charge Customer service may be automatically changed to categories of service that are available in such new location with no refund available For purposes of the Gold Service all references to time will mean the Customer s local time References to the United States include the continental United States Alaska Hawaii and Puerto Rico Whole Unit Replacement If the telephone technician determines that the defective unit is one that is easily disconnected and reconnected or if the technician determines that Customer Product is one that should be replaced as a whole unit Velocityll reserves the right to request that the defective unit be shipped to Velocity11 for repairs Under the Standard Warranty Velocity11 will repair the unit and ship it back to Customer Parts Ownership All service parts removed from Customer Product s become Velocityll property Customer must pay the current retail price s for any service parts removed from Customer Product s and retained by Customer 54 PlateLoc User Manual Appendix A EXHIBIT B OPTIONAL SUPPORT SERVICES Platinum Service Plan The Platinum Service Plan is available to each customer at an additional cost Under the Platinum Service Plan the Customer is guaranteed a four hour response time for all repair service calls When required a Service Technician will be dispatched the same day the request was initiated 365 days of the
38. machine The PlateLoc is an automated piece of equipment with moving parts Ensure WARNING Install the Serial Communications Cable For automated control users only If you are only using the PlateLoc manually skip this NOTE section The serial communications cable is only necessary if you wish to command the PlateLoc remotely or if the PlateLoc is part of an integrated robotic system 1 Plug one end of the serial communications cable into the RS 232 serial communications port on the rear of the PlateLoc see figure 1 2 Plug the other end of the serial communications cable into the available serial port on the PC you will use to control the PlateLoc 10 PlateLoc User Manual Chapter 2 Installation Setting up the PlateLoc Mounting the Seal Roll is on Before mounting the seal roll ensure that the power is off and the air pressure CAUTION d P 1 Remove the seal roll seal roll hubs and axle from their packaging 2 Take one of the seal roll hubs and screw it 15 to 20 turns onto the seal roll axle Figure 3 Seal Roll Axle and Hubs SealRollHubs Seal Roll 3 Place the seal roll onto the axle see figure 3 The roll should fit snugly against the seal roll hub 4 Screw the second hub onto the axle Tighten until the hubs are securely holding the roll 5 Using scissors make a clean straight cut across the end of the seal material cutting off any part of the sea
39. material 11 feeding problems 47 loading 12 seal quality 21 seal roll axle 11 changing 38 hubs 11 mounting 11 slot 13 supports 11 Seal Temp 19 seal transfer plate 40 vacuum error 48 seal transfer plates 13 sealing problems 46 serial communications 10 error 50 port 10 service 6 phone number 45 Service Center 5 Support Services 51 Service button 19 Set Parameters Menu 14 Setup button 19 State Codes 27 Status 15 support insert 17 system requirements 5 T temperature errors 49 troubleshooting 45 tubing 9 U UL6 unpacking 7 V vacuum error 48 Ww warranty 51 website 5 wells 17 57
40. me Description Retrieves the current sealing time set in the PlateLoc s memory If an error occurs GetSealingTime will return 1 and an error event will be fired Parameters None Returns double Example Visual Basic Visual C PlateLocl Initialize 2 sets the COM port to COM2 Dim sealingtime As Double sealingtime PlateLocl SealingTime PlateLocl Initialize 2 sets the COM port to COM2 double fSealingTime fSealingTime PlateLocl GetSealingTime 25 PlateLoc User Manual Chapter 4 Active X Documentation SetSealingTime Description Sets the amount of time that the hot plate contacts the sealing foil Valid numbers are from 0 5 to 12 0 seconds inclusive If a sealing time less than 0 5 seconds is specified the sealing time will automatically be set to 0 5 seconds and an error event will be fired Similarly if a sealing time greater than 12 0 seconds is specified the sealing time will automatically be set to 12 0 seconds and an error event will be fired Parameters double Returns None Example Visual Basic Visual C PlateLocl Initialize 2 PlateLocl Initialize 2 sets the COM port to COM2 sets the COM port to COM2 Dim sealingtime As Double double fSealingTime 2 3 sealingtime 2 3 PlateLocl SetSealingTime fSealingTime PlateLocl SealingTime sealingtime short State State contains information about the PlateLoc s current
41. ng Seal Material into a Hot Machine on page 39 Press the silver gripper release button see figure 5 You should hear the gripper snap closed Open the seal gripper by pushing the lower seal gripper down until it clicks into place see figure 5 Using a pen or marker mark the seal material 7 from the end Feed the material into the seal roll slot directly above the lower seal gripper see figure 5 If you have problems loading the seal ensure that the edge entering the machine has not curled down Curl the edge up by hand to remove any downward curl NOTE Look into the front opening of the machine After a few inches of seal material have been fed in you should see the seal material emerging into the main sealing chamber Keep feeding the seal material until the 7 mark just disappears Ensure that the emerging edge of the seal is centered Close the seal gripper by pressing the silver gripper release button under the gripper You should hear the gripper snap closed Gently roll any slack back up into the seal roll Be careful not to pull the seal out of the machine The seal should look like the example in figure 6 when loaded correctly Figure 6 Properly Loaded Seal Roll T Loaded L Seal Material N N PlateLoc 13 PlateLoc User Manual Chapter 2 Installation Cold Running the PlateLoc After installing the PlateLoc the machine should be cycled
42. njury do not remove PlateLoc WARNING covers 37 PlateLoc User Manual Chapter 5 Maintenance Cleaning the Hot Plate The hot plate remains EXTREMELY HOT during cleaning Use a THICK rag WARNING when cleaning to keep exposed skin safely away from the hot plate 1 Remove the plate from the stage Do not turn off the machine The hot plate is easier to clean when kept hot 2 The hot plate is located on the roof of the main sealing chamber Using a thick clean dry rag reach into the front opening of the PlateLoc and carefully wipe off built up residue from the hot plate DO NOT use any type of metal object to clean the hot plate Doing so will CAUTION damage the hot plate and adversely affect the seal quality Changing the Seal Roll The seal roll only needs to be replaced when a roll empties To replace a seal roll follow the steps for removing the seal roll listed below After removing the empty seal roll it is recommended to let the machine cool down to 30 C before following the procedures outlined in Mounting the Seal Roll on page 11 and Loading the Seal on page 12 Sometimes it is necessary to reload seal material without cooling down the machine This may occurr if the PlateLoc displays an error and instructs you to reload the seal material The need also arises if the seal roll empties in the middle of an automated process To reload seal material without cooling down the machine refer to Reload
43. o the system If you have any questions contact your facilities department or a Velocity11 service representative Before installing the compressed air line ensure that the air system can consistently supply 2 5 cfm at 90 psi to the PlateLoc Any drop in air pressure may reduce the performance of the unit Do not allow the input air pressure to exceed 145 psi The PlateLoc cannot STOP CAUTION withstand pressure above 145 psi Additionally confirm that the air supply is clean dry air The NAFD2000 0 01 um air filter is included in your PlateLoc packaging This filter must be installed in the air line going to the PlateLoc A detailed instruction sheet on installing the NAFD2000 0 01 um air filter is included in your PlateLoc packaging Ensure that the air line coming into the PlateLoc is filtered from moisture and CAUTION oil Significant moisture or oil can adversely affect the life of the unit The NAFD2000 air filter is included with the PlateLoc to help filter out excess oil from the air flow CAUTION Switch off air supply before connecting the air line to the PlateLoc 1 Turn the pneumatic ON OFF switch on the rear of the PlateLoc to the OFF position toward the outside of the machine See figure 1 2 Cut two lengths of 1 4 polyurethane tubing to the length you need for the air line PlateLoc User Manual Chapter 2 Installation 3 Ifyour air supply uses a threaded fitting attach the 1 4 NPT fitting to the nozzle A
44. older has been specified click Next The Select Program Folder screen will appear 8 Click Next if you want the specified folder If not select a new folder and then click Next 9 The Setup Status Screen will appear When InstallShield Wizard Complete appears click Finish 10 Follow the directions for your particular development environment for adding an ActiveX to your application The PlateLoc ActiveX has been installed in the folder you specified You are now ready to run the PlateLoc ActiveX from your computer 23 PlateLoc User Manual Chapter 4 Active X Documentation Properties short DesiredTemperature DesiredTemperatur GetDesiredTemp is the temperature in Celsius needed to seal a plate rature Description Retrieves the current temperature set point in Celsius saved in the PlateLoc If an error occurs GetDesiredTemperature will return 1 and an error event will be fired Parameters None Returns short Example Visual Basic Visual C PlateLocl Initialize 2 PlateLocl Initialize 2 sets the COM port to COM2 sets the COM port to COM2 Dim desiredtemp As Short short iDesiredTemp desiredtemp iDesiredTemp PlateLocl DesiredTemperatur PlateLocl GetDesiredTemperature SetDesiredTemperature Description Parameters Returns Example Sets the temperature in Celsius used for a sealing cycle Valid numbers are from 20 to 230 inclusive If
45. on Chapter 4 Active X Documentation The PlateLoc is designed to allow easy integration into an automated system An ActiveX control is provided that installs on your computer simplifying the programming needed to control the PlateLoc from your system This document provides information on how to use Velocityll s ActiveX control within your Visual Basic or Visual C application Please note that this documentation is for ActiveX version 3 1 and that all example code assumes that the PlateLoc control is instantiated as PlateLocl NOTE For manual operation of the PlateLoc please see Manually Sealing a Microplate on page 20 Installing PlateLoc Software 1 Take the compact disc marked PlateLoc ActiveX from its case and insert it into the CD drive on your PC For the highest performance from your PlateLoc we recommend a Pentium 166 or better based NOTE PC running Windows NT 4 0 Service Pack 6 or Windows 2000 2 Double click Setup exe The InstallShield Wizard will appear 3 Click Next The Customer Information screen will appear 4 Enter your name company name and PlateLoc serial number in the appropriate fields 5 Click Next The Choose Destination Location screen will appear 6 The installation program will install the program on your C drive unless you otherwise specify a location To specify a location click Browse and choose the desired destination folder Otherwise click Next and skip to step 9 7 When the f
46. ool before reaching inside to clean it Reaching WARNING inside the PlateLoc while it is still warm is extremely dangerous 2 Run the machine without a plate and with the temperature set to 30 C 3 Remove the piece of seal material and ensure that it has a nice clean edge 4 Repeat the process three or four times 5 Ifyou repeatedly notice the seal material has a frayed or poorly cut edge the cutter on your PlateLoc may have dulled or may have shifted to an incorrect position Please contact Velocity 11 s Service Center at 650 846 6500 Lubricating the Door Hinges 1 Turn off power to the machine 2 Switch the pneumatic ON OFF switch on the back of the machine to OFF toward the outside of the machine 3 Remove the plate plate support insert and plate locator from the stage 4 With the stage extended carefully apply one drop of a standard lubricant Tri Flow 3 in 1 to the side of the door hinges located just on the inside of the main chamber see figure 19 Be sure to apply the lubricant to the side of the door hinge and allow the lubricant to run down into the door hinge axle the rod and spring connecting the two door hinges Figure 19 Lubricating the Door Hinges M 4 5 Open and close the door repeatedly by hand to help spread the lubricant 6 Wipe off excess lubricant from the machine interior 43 PlateLoc User Manual Chapter 5 Maintenance 44 PlateLoc User Manual Chapter 6
47. pletes successfully Ifthe cycle completes successfully continue with normal use of the PlateLoc Ifthe same error repeats multiple times there may be a problem with the hot plate vacuum system Ifa new error appears find the error in the list of PlateLoc error messages listed on the following pages Transfer Plate Vacuum Error The transfer plate did not detect the seal material in a correct position for feeding Remove the seal from the rear of the machine If the material is difficult to remove refer to Seal Material Feed ing Problems on page 47 e Cut off any tattered edges and reload the seal material into the machine Refer to Reloading Seal Material into a Hot Machine on page 39 e Try running the PlateLoc with a spare plate to see if the cycle completes successfully Ifthe cycle completes successfully continue with normal use of the PlateLoc Ifthe same error repeats multiple times there may be a problem with the transfer plate vacuum system Actuator Timeout Error The transfer plate did not complete one of its movements in the allotted time e Check if the seal roll spins with only moderate resistance in the roll supports Refer to Seal Material Feeding Problems on page 47 e Check if there is a cut piece of seal material inside the machine fthere is a scrap the transfer plate was obstructed from returning to its home position in the rear of the machine Remove the scrap piece of seal material
48. s For the product warranty for this PlateLoc please refer to Velocity11 s Product Warranty on page 51 Warnings and Advisories Your safety while utilizing the PlateLoc is of paramount importance to Velocityll Carefully read and follow all warnings and cautions that are outlined in this manual The PlateLoc must only be used in the manner in which it was intended by Velocity11 Any other use of the PlateLoc may cause damage to the product samples or harm to the user Take note of the following signs They call attention to a procedure that if not correctly performed could result in a hazardous condition or damage all or part of the product Do not proceed beyond a warning label until the indicated conditions are fully understood and met Warning signs signal procedures that will potentially cause bodily harm or death if not performed correctly Caution signs signal procedures that will potentially cause damage to the CAUTION instrument if not performed correctly Note signs indicate points that are important in order to correctly setup or use the PlateLoc No danger is involved This unit is currently being evaluated and tested for full CE and UL certification Any modifications or changes to the PlateLoc not expressly approved in this manual could void your warranty and authority to operate this equipment PlateLoc User Manual Chapter 2 Installation Chapter 2 Installation Unpacking the PlateLoc The follow
49. s for your PlateLoc setup is to experiment with various times and temperatures until you find settings that provide a consistently good seal for your plates Locate your plate material and seal material in table 2 Use the standard seal time and seal temperature provided as a starting point from which to determine the ideal temperature and time for your machine Table 2 Seal Times and Temperatures Plate Type Peelable Seal Piercable Seal Clear Seal temp 180 C 180 C 150 C Polystyrene time 1 2 s 1 2s 2 0 s temp 170 C 170 C 150 C Polypropylene time 12s 1 25 2 0s When testing times and temperatures it is recommended to hold the temperature constant and NOTE cycle through a few different seal times since the PlateLoc takes some time to warm up and cool down Check the Velocity11 web page for updates and further details on seal materials seal temperatures and seal times To determine if your settings have produced a good seal refer to Checking Seal Quality on page 21 Manually Sealing a Microplate 1 Ensure that all of the setup procedures have been followed 2 Ensure that the temperature and sealing time have been set satisfactorily 3 Ensure that the seal you have chosen is applicable for your kind of microplate 4 Place a microplate in the plate locator It should fit easily 5 When the Status reads Ready on the Main Menu of the LCD touch screen see figure 12 press RUN NOTE Do not
50. sDialog PlateLocl Initialize 2 sets the COM port to COM2 PlateLocl ShowDiagsDialog 31 PlateLoc User Manual Chapter 4 Active X Documentation The following screen will appear when ShowDiagsDialog is called see figure 13 Figure 13 PlateLoc Diagnostics Screen PlateLoc Diagnostica SedTme Curent Seal Time 2 0 rec New Seal Tink 7 sec Set Tine Seal T anperalue Cunerk Sea Terps IT Hew Seal Temperature fet Close Description Closes serial port used by the PlateLoc ActiveX After calling Close the method Initialize must be called again before resuming communications with the PlateLoc Parameters None Returns None Example Visual Basic Visual C PlateLocl Initialize 2 PlateLocl Initialize 2 sets the COM port to COM2 sets the COM port to COM2 PlateLocl SealingTime 2 3 PlateLocl SetSealingTime 2 3 PlateLocl Close PlateLocl Close Free up the serial port Free up the serial port 32 PlateLoc User Manual Chapter 4 Active X Documentation StartCycle Description Parameters None Returns None Example Starts the PlateLoc sealing cycle Visual Basic Visual C PlateLocl Initialize 2 sets the COM port to COM2 PlateLocl StartCycle PlateLocl Initialize 2 sets the COM port to COM2 PlateLocl StartCycle
51. state of operation GetState Description Retrieves the PlateLoc state code Refer to table 3 for a list of valid state codes Parameters None Returns short Example Visual Basic Visual C PlateLocl Initialize 2 PlateLocl Initialize 2 sets the COM port to COM2 sets the COM port to COM2 Dim state As Short short iState state PlateLocl State iState PlateLocl GetState SetState Not implemented 26 PlateLoc User Manual State Codes Chapter 4 Active X Documentation Table 3 State Protocol Definitions Status Code Definition 0 Ready 1 Running 2 OutOfTemp 3 Error 4 Setup Screen 5 Password Screen 6 Service Screen service use only 7 AD Values Screen service use only 8 Actuation Screen service use only 10 Threshold Screen service use only 11 Timing Screen service use only 12 Return Actuators 13 Debounce 14 Waiting to Run 15 Config Screen service use only 16 Fatal Error 17 Temperature Screen service use only short ErrorFlags GetErrorFlags Description Parameters None Returns short 27 ErrorFlags contain information about the last error asserted by the PlateLoc A summary of the error flags is given in table 4 Retrieves the PlateLoc s error flags PlateLoc User Manual Chapter 4 Active X Documentation Example Visual Basic Visual C PlateLocl Initi
52. ttach the 1 4 one touch hose fitting into the 1 4 tubing If your lab already uses 1 4 tubing use the T fitting to connect to the existing tubing as close to the source as possible supply line refer to the sheet Installing Your NAFD2000 Air Filter included with your PlateLoc For more detailed instructions on installing the NAFD2000 air filter with your air Q5 CAUTION 4 Attach the other end of the 1 4 tubing to the quick relesase input of the included NAFD2000 air filter see figure 2 Tug gently on the line If you feel resistance the line has been properly installed 5 Attach one end ofa second length of 1 4 tubing to the quick release output of the NAFD2000 air filter Tug gently on the line to ensure it has been properly installed The ARROW on the top side of the NAFD2000 filter indicates the direction in CAUTION which air should flow through the filter Attaching the filter incorrectly will cause damage to the filter Figure 2 Air Line Layout Diagram The ARROW on the top of the filter indicates the direction in which air should flow through the filter pi amp From Air Supply X aau ee E Air Input Port Automatic Bleed Valve PlateLoc 6 Insert the other end of the 1 4 tubing into the quick release air input port on the back of the PlateLoc see figure 1 7 Tug gently on the air line after installing it into the PlateLoc If you feel resistance then t
53. utton in the Set Parameters menu to return to the Main Menu see figure 12 18 PlateLoc User Manual Chapter 3 Plate Sealing Setting Seal Time and Temperature 1 Press the Setup button on the Main Menu This will take you to the Set Parameters Menu see figure 12 2 There are two parameters to set Seal Time and Seal Temperature These parameters differ for the type of seal and plate being used see table 2 To adjust the parameters simply press the up or down arrow on the LCD touch screen next to the parameter you wish to adjust 3 Press Done to return to the Main Menu 4 The PlateLoc will take approximately 2 5 minutes to reach sealing temperature NOTE The RUN button remains blank until the temperature reaches the required level Figure 12 Menus Main Menu EN Set Parameters Menu Seal Time 1 28 Set Seal Time Seal Temp 170 C Status set Seal Temp I _ Setup Service xis Done a ua la VELOCITY II The Service button is a password protected entrance into Velocity11 S service system The NOTE operator will not have access to the service system 19 PlateLoc User Manual Chapter 3 Plate Sealing Standard Seal Times and Temperatures The PlateLoc is designed to be used with as many different plates and seal materials as possible Each type of plate and each type of seal will require slightly different seal times and seal temperatures The best way to determine setting
54. within the first year will be covered under Velocity 11 s initial one year warranty See Appendix B Velocity11 s Product Warranty on page 51 After the first year your warranty can be extended through one of Velocity11 s service plans See Warranty Information and Service Contracts on page 6 for information regarding service plans General Maintenance Schedule Use the following table to determine the maintenance needs of your PlateLoc based on observed symptoms and the approximate schedule After determining your maintenance need follow the steps below to properly clean your machine The maintenance schedule is approximated for expected regular use of the PlateLoc up to 150 plates day Heavier use may require more frequent maintenance Table 7 PlateLoc Maintenance Schedule Maintenance Need Time Schedule Symptoms Clean the Hot Plate Inspect once a week Hot Plate Vacuum Error Plate sticks to the hot plate after sealing Change the Seal Roll Empty seal roll Overall Machine Cleaning Once a month Dust and grime on machine exterior and interior Change the NAFD2000 Filter Varies nsufficient Air Pressure Error Saturated Filter Inspect the Cutter Once a month The seal material lacks a clean edge or does not cut Lubricate Door Hinges Once every six months Door squeaks or moves slowly Door does not close completely To avoid electrical shock and possible severe i
55. years For shippable items Velocity 1 will ship out a replacement product within twenty four hours The Customer will be responsible for shipping the damaged product back to Velocityll in three working days after receiving the replacement product from Velocityll The Customer may upgrade to this plan during the first year of service Please contact your Velocityll representative for more details 35 PlateLoc User Manual Appendix A 56 PlateLoc User Manual Index Index A ActiveX 23 Diagnostics Screen 32 Error Strings 29 Events 34 Install 23 Methods 31 Properties 24 SCodes 34 advisories 6 air 8 filter 8 input port 9 pneumatic ON OFF switch 10 problems 46 quick connect 7 9 requirements 5 C CE 6 certification 6 chimney 17 21 cleaning 41 cold cycle 15 control panel air connections 8 covers 45 cutter inspecting the 43 D door close error 49 door hinges 37 43 E error messages 48 F filter 8 arrow 9 changing the 41 G gripper release button 13 H hot plate 39 cleaning 38 plate sticks to 46 safety 38 vacuum error 48 installation 8 L LCD touch screen 14 18 problems 45 lower 13 lubricate 37 43 M main chamber 40 43 Main Menu 14 maintenance 37 schedule 37 manual sealing 20 memory errors 49 P plate locator 17 pneumatic gauge 10 polyurethane tubing 9 power 120VAC 10 cable 10 problems 45 switch 10 R regular use 37 S seal gripper 12 seal
Download Pdf Manuals
Related Search