Technical Manual - Elchrom Scientific AG
Contents
1. xe Elchrom Scientific Excellence in Electrophoresis www elchrom com 1 5 Technical features Integrated Peltier elements for buffer cooling heating Integrated temperature probe to automatically control buffer temperature Range 4 55 C t constant running buffer temperature Integrated pump for buffer circulation to eliminate temperature and pH gradients c laminar buffer flow and constant pH even in the vicinity of the electrodes Integrated pump start delay controller t allows DNA RNA to enter gel matrix before buffer circulation starts Unique double wired Platinum Iridium electrode amp homogeneous electric field Drainage system for easy buffer exchange 1 6 Performance Versatility in combination with Elchrom Scientific s ready to use gels the ORIGINS by Elchrom guarantees optimal results for different applications such as microsatellites RFLP SSCP heteroduplex analysis next generation sequencing LAM PCR multiplex PCR high throughput PCR checking and many others Unparalleled reproducibility all electrophoretic parameters are tightly controlled to insure identical running conditions for all the gels High resolution with Elchrom s proprietary gel matrix size differences of down to 1 bp can be separated on only 8 cm running distance High sensitivity Elchrom s ready to use gels are significantly more sensitive than agarose gels as low as 0 05 ng band Fast runs2. change without notice Payment Terms within Switzerland Terms of sale are net 30 days from date of the invoice Elchrom Scientific AG maintains the ownership of all delivered products until the invoice is fully paid Shipping Standard delivery time will be 1 3 weeks from receipt of your order For large quantities please contact us directly for a better estimation of delivery time Inspection and Returns Upon receipt please inspect the goods immediately within three days and notify our Customer Services Department for any claims such as shortage defects or damages Authorisation for return shipments needs to be approved on beforehand by our Customer Services Department No credit will be granted on returns that have not been priorly authorised Certain products cannot be authorised for return shipments due to temperature and packaging restrictions Warranty Elchrom Scientific AG warrants that all products will perform according to the specifications stated on the Quality Control Certificate up until their respective expiry dates and will repair any defective product We will not replace products that do not perform due to misuse or inappropriate storage No other warranties are expressed or implied including but not limited to the implied warranties of merchantability and fitness for any particular purpose Elchrom Scientific AG is not liable for any incidental indirect special exemplary or consequential damages All produ
3. of the gel 3 Move the Catamaran close to the gel until it rests on the plastic backing 4 Lower the other side of the Catamaran until it entirely frames the gel and both sides sit precisely on the plastic backing ORIGINS by Elchrom Technical Manual 11 2012 Page 17 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com The gel can be repositioned by carefully shifting the Catamaran Similarly the gel can be shifted closer to one or the other electrode Wide Mini S 4x13 and S 4x25 AV These gel formats consist of 4 gels on one presentation On each side _ lt the two gels are separated by a little gap When positioning those gels the opening in the Catamaran should align perfectly with the gap between the upper and lower gels to ensure proper buffer circulation 5 Check the correct Catamaran position Both sides of the Catamaran are located on top of the gel backing and not on top of the gel The row of sample wells must be parallel to the electrodes Correct Position Wrong Position Precast Gel Buffer Level 3 Buffer level Ideally the buffer level should be 2 to 3 mm above the upper electrode wire A blue white mark indicates the correct buffer level see left side of gel compartment If necessary adjust buffer with a 20 50 ml syringe 4 Sample loading NZ _ lt Before loading any samples switch off the pump Load samples with a 1 20 ul pipette The optimal load
4. temperature control allows high voltage amperage runs without the negative effects of increased buffer temperature High throughput up to 400 samples per run application dependent Cost effective ORIGINS by Elchrom offers a cost effective alternative to capillary sequencing since it allows the analysis of PCR fragments generated without fluorescence labeled primers ORIGINS by Elchrom Technical Manual 11 2012 Page 6 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 2 Set Up 2 1 Packaging content ORIGINS by Elchrom Easy Stain Gel Tray P N 2344 Peel IT P N 2355 Power cord CH EU or USA 2 power cords for Power Supply black P N 2100 501 red P N 2100 502 Flex tube with quick fit for buffer drainage P N 2100 503 Catamaran S 8 Mini gel P N 2008 Catamaran S 12 Mini gel P N 2012 Catamaran S 50 100 Wide Mini gel PN 2020 Special forceps PN 2366 4x50 ml of 40 x running buffer Technical manual Short technical manuals for 9 20 and 55 C Warranty Certificate of Conformity Unpack the equipment and accessories carefully and keep the packaging material for future transports or storage Check all equipment and accessories for damages caused during transportation Should you identify any damages please inform Elchrom Scientific and our local representative immediately Claims for missing parts defects or damages should be pla
5. the gel in running buffer for pre warming 4 7 Exchanging the running buffer To exchange the running buffer of the ORIGINS by Elchrom connect the flex tube for buffer drainage to the out let for the running buffer See 7 in section 3 1 Note Buffer drainage starts immediately after the flex tube is connected To suspend buffer drainage disconnect the flex tube by pressing the corresponding button on the quick fit connection The tube can be removed without buffer spill Ethidium Bromide Caution Ethidium Bromide is a powerful mutagen Wear appropriate protective clothing gloves and safety googles Solutions and gels stor contaminated with EtBr must be disposed of in compliance with local regulations For safe EtBr disposal Elchrom Scientific recommends the use of Bind ET an efficient and easy to use EtBr removal system P N 2350 Before refilling the unit with fresh 1 x TAE running buffer it is recommended to clean the ORIGINS by Elchrom with ddH20 turn on the circulation pump for 5 min For profound decontamination please refer to protocol 2 on our web page elchrom support protocols ORIGINS by Elchrom Technical Manual 11 2012 Page 20 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 5 Accessories 5 1 Catamarans Catamarans are plastic frames designed to properly position ready to use gels in the ORIGINS by Elchrom In addition they prevent the gel from flo
6. 230 V EU plug 2100U ORIGINS by Elchrom 115 V US plug ORIGINS by Elchrom Technical Manual 11 2012 Page 10 of 31 Cy Elchrom Scientific Excellence in Electrophoresis www elchrom com 3 2 Power cord connection Legend 1 On Off button 2 Fuse 5 AT 3 Power cord 115 230 V AC 4 Serial number 3 3 Display Legend ORIGINS 1 Temperature settings a actual temperature of running buffer b set temperature of running buffer 2 Circulation pump settings a pump on off b pump delay 0 minutes no delay 1 5 minutes delay 4 5 minutes delay 12 minutes delay 3 Timer settings a timer on off b set time elapsed time will be indicated left to the setting Menu navigation 4 Navigate through the menu temperature pump timer settings 5 Change value of parameter 6 Confirm the selected value ORIGINS by Elchrom Technical Manual 11 2012 Page 11 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 3 4 Exchanging the running buffer The ORIGINS by Elchrom is equipped with a so called quick fit connection to drain the running buffer from the unit It allows for easy and spill free running buffer exchange The valve is located on the left side of the electrophoresis unit See Hl in section 3 1 3 5 Electrode arrangement The anode and cathode of the ORIGINS by Elchrom consist of 2 parallel Platinum Iridium wires 1 and 7 mm above the gel t
7. Ay Important 205 Please follow the instructions indicated to avoid unnecessary mistakes and or errors ORIGINS by Elchrom Technical Manual 11 2012 Page 4 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 1 3 Important user information To ensure best performance of ORIGINS by Elchrom please read the following instructions carefully before operating the apparatus or other system components for the first time The technical manual describes the use of the ORIGINS by Elchrom ready to use gels and accessories and other additional tools It thereby focuses on instructing the use of the apparatus in combination with Elchrom s ready to use gels The use of casting trays for agarose gels is mentioned briefly in 5 2 Elchrom Scientific reserves the right to make changes to the following instructions without notification For the latest version of the technical manual please refer to our web page www elchrom com 1 4 Product information Type 2100CH 230 V 50 Hz CH plug 2100E 230 V 50 Hz EU plug 2100U 115 V 60 Hz US plug Operating voltage 115 or 230 V AC Power consumption 260 W Electrodes Platinum Iridium 120 mm distance between cathode and anode Main power fuse 5A Pump delay min 0 1 5 4 5 12 Z Dimensions Width x depth x hight 550 x 410 x 220 mm Weight 18 kg Gel compartment 108 x 278 mm Buffer volume 1 9 ORIGINS by Elchrom Technical Manual 11 2012 Page 5 of 31
8. Excellence in Electrophoresis www elchrom com ORIGINS BY ELCHROM SCIENTIFIC P N 2100CH ORIGINS by Elchrom 230 V CH plug P N 2100E ORIGINS by Elchrom 230 V EU plug P N 2100U ORIGINS by Elchrom 115 V US plug ORIGINS by Elchrom Technical Manual 11 2012 K Elchrom Scientific Excellence in Electrophoresis www elchrom com Table of Content 1 General Information 4 1 1 Introduction 4 1 2 Symbols 4 1 3 Important user information 5 1 4 Product information 5 1 5 Technical features 6 1 6 Performance 6 2 Set Up 7 2 1 Packaging content 7 2 2 Positioning 7 2 3 Safety instructions 8 2 4 Installation 8 3 User Manual 10 3 1 The ORIGINS by Elchrom Scientific 10 3 2 Power cord connection 11 3 3 Display 11 3 4 Exchanging the running buffer 12 3 5 Electrode arrangement 12 4 Electrophoresis 13 4 1 Preparing ready to use gels 13 4 2 Preparing TAE running buffer 14 4 3 Preparing DNA samples 15 4 4 Preparing the ORIGINS by Elchrom 16 4 5 Running ready to use gels 17 ORIGINS by Elchrom Technical Manual 11 2012 Page 2 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 4 6 High resolution electrophoresis with Spreadex and 20 Poly NAT gels 4 7 Exchanging the running buffer 20 5 Accessories 21 5 1 Catamarans 21 5 2 Agarose gel casting trays 22 5 3 Special forceps 23 5 4 Peel IT 23 5 5 Easy Stain Gel Tray 24 6 Customer Sample Analysis Demo
9. Gel Tray and the plastic backing washed off in the staining solution Except for pre stained PCR CheckIT gels we recommend to detach all gels from the backing to increase staining and destaining efficiency Elchrom Scientific provides a Destaining Solution P N 3037 to further reduce possible background For staining Elchrom Scientific recommends to use SYBR Gold SYBR Green or EtBr However alternative dyes may be used ORIGINS by Elchrom Technical Manual 11 2012 Page 19 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 4 6 High resolution electrophoresis with Spreadex and Poly NAT gels High resolution gel electrophoresis is normally performed on Spreadex and 9 or 12 Poly NAT gels for very short fragments Short Fragment gels may be recommended The gels are run at 55 C to eliminate anomalous sequence dependent DNA migration Furthermore elevated temperatures significantly reduce running time approximately by a factor of 2 at 55 C Ready to use gels intended to run at 55 C do require sequential pre warming see 4 1 to RT for 20 to 30 minutes to 55 C for 30 minutes Pre warming is essential to prevent the formation of small bubbles within the gel matrix during the run Those may disturb the banding pattern and influence resolution AVA For pre warming place the gel ON TOP of the Catamaran in the pre A 205 warmed ORIGINS by Elchrom Do not submerge
10. TAE buffer Ideal buffer level indicated by a blue white mark on the left hand side of the ORIGINS by Elchrom Electrophoresis parameters The correct buffer composition and fill level for electrophoresis should result in the following values for voltage and amperage slight discrepancies can be adjusted by adding ddH20 or by removing buffer 20 C 120 V 10 V cm 350 mA 50 mA 55 C 120 V 10 V cm 700 mA 100 mA 9 C 108 V 9 V cm 250 mA 50 mA 9 C 72 V 6 V cm 170 mA 20 mA Note Using running buffers different from TAE with ready to use gels from Elchrom Scientific will result in inferior results SE Ready to use gels by Elchrom Scientific are NOT compatible with Pi borate containing buffers ORIGINS by Elchrom Technical Manual 11 2012 Page 14 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com Running buffer durability In average the running buffer can be re used up to three times However durability can vary depending on running conditions Such as temperature time amperage etc Low buffer capacity and quality may reflect in blurry DNA RNA bands and reduced resolution In this case the running buffer should be changed before the next run Running buffer for PCR CheckIT PCR CheckIT gels may alternatively be equilibrated to a running buffer different from TAE Equilibration to the alternative buffer is performed in the ORIGINS by Elchrom for at least 2 hour
11. ace Always keep the work bench of the ORIGINS by Elchrom clean and d free of small movable objects such as paper sheets pipette tips etc The ventilation system of the ORIGINS by Elchrom generates a g remarkably strong air influx Free moving small object may therefore be sucked into the ventilation and damage the rotor or block cooling Temperature Ambient temperature should be between 15 and 28 C Avoid temperature fluctuations and keep the unit away from heating devices lt Environment The working environment should be free of dust smoke and corrosive fumes The unit must not be operated in a potentially explosive atmosphere lt D Light exposure Protect from direct sun light overheating Use sufficient light for gel manipulation and loading m ORIGINS by Elchrom Technical Manual 11 2012 Page 8 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com d Placement Place the unit firmly on a suitable and stable surface 0 ORIGINS by Elchrom Technical Manual 11 2012 Page 9 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 3 User Manual 3 1 The ORIGINS by Elchrom Scientific Legend 1 On Off button see below 2 Cords to power supply 3 Display 4 Navigation for display 5 Electrodes 6 Cover 7 Outlet for running buffer P N Product description 2100CH ORIGINS by Elchrom 230 V CH plug 2100E ORIGINS by Elchrom
12. al 11 2012 Page 21 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com Catamaran S 208 400 P N 2021 HT size 10 8 x 13 5 x 2 7 cm To position 1 Wide Mini High throughput gel S 2x104 S 2x104L S 2x200 Catamaran S 13 100 P N 2021 SF Size 10 8 x 26 5 x 2 7 cm To position 1 Wide Mini gel of Short Fragment gels SF50 S 2x2 S 2x3 S 2x4 S 2x13 S 2x25 S 4x25 5 2 Agarose gel casting trays To run self made agarose gels in the ORIGINS by Elchrom special casting trays are required to allow buffer circulation The trays therefore possess circulation slots located 5 mm above the bottom of the tray Elchrom Scientific provides two different tray sizes each to take two Teflon combs For pouring the trays are sealed with tape agarose temperature lt 60 C UV transparent mini agarose gel tray P N 2040 Inner dimensions 7x 10 7 cm maximum gel volume 22 ml Running distance 10 cm with 1 comb 2 x 4 7 cm with 2 combs Combs 10 wells 1 5 mm thick 5 mm wide P N 2041 16 wells 1 5 mm thick 3 5 mm wide P N 2042 In the ORIGINS by Elchrom 3 mini agarose gel trays can be run simultaneously UV transparent wide mini agarose gel tray P N 2045 Inner dimensions 12 8 x 10 7 cm maximum gel volume 40 ml Running distance 10 cm with 1 comb 2 x 4 7 cm with 2 combs ORIGINS by Elchrom Technical Manual 11 2012 Page 22 of 31 FTO Elchrom Scientific Excellence in Electrophoresis
13. ank bottom respectively Arranging the electrodes in the same panel as the gel leads to an unsurpassed homogeneous electric field Simultaneously this special geometry achieves a higher effective current density compared to a standard electrophoresis tank The distance between the anode and the cathode is 12 cm This short distance creates substantially higher field strength at identical voltage setting than the longer distance in standard submarine units ORIGINS by Elchrom Technical Manual 11 2012 Page 12 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 4 Electrophoresis Please also follow the tutorial movie on www elchrom com 4 1 Preparing ready to use gels 1 Choose the appropriate gel type for your application To identify the best option please refer to Elchrom s on line software ElQuant and How to find the best gel on www elchrom com 2 Remove the aluminium bag by cutting one short side carefully with scissors 3 Remove the inside plastic bag containing the gel and place it on the work bench Caution some gels may be immersed in a solution containing Ethidium Bromide EtBr 4 Cut the plastic bag on three sides and carefully remove the gel by gripping the plastic backing with the special forceps provided with the ORIGINS by Elchrom 5 All ready to use gels need to be pre warmed to the relevant temperature prior to the run 9 C No pre warming 20 C Allow to r
14. ating and orientate it perpendicularly to the electric field Catamarans also optimise buffer circulation by directing it homogeneously across the gel surface These factors guarantee uniform and constant temperature independent of running time and therefore ensure highly reproducible results There is a variety of different Catamarans fitting to all different gel types Mini Wide Mini high throughput and Short Fragment gels Catamaran S 50 100 P N 2020 size 10 8 x 26 5 x 2 7 cm To position 1 Wide Mini presentation S 2xX13 S 2x25 S 4x13 S 4x25 Catamaran S 13 50 P N 2015 size 10 8 x 13 5 x 2 7 cm To position 1 Wide Mini gel consisting of 1 gel S 2x13 S 2x25 or 2 gels S 4x13 S 4x25 Opening to allow efficient flushing of the channel between the gel sections in the S 4x25 and S 4x13 gels Catamaran S 8 P N 2008 Size 10 8 x 7 x 2 7 cm To position 1 Mini gel for 8 samples 8 7 cm running distance Slot of 3mm allows flow of running buffer over gels Catamaran S 12 P N 2012 Size 10 8 x 10 x 2 7 cm To position 1 Mini gel for 12 samples 5 7 cm running distance Catamaran S 208 400 P N 2020 HT Size 10 8 x 26 5 x 2 7 cm To position 1 Wide Mini High throughput presentation S 2x104 S 2x104L S 2x200 Catamaran S 50 100 P N 2020 SF To position 1 Wide Mini presentation of Short Fragment gels SF50 S 2x2 S 2x3 S 2x4 S 2x13 S 2x25 S 4x25 ORIGINS by Elchrom Technical Manu
15. ced with Elchrom Scientific within 3 days after receipt Do NOT return equipment or accessories without prior instructions from Elchrom Scientific AVA AAN a 2 2 Positioning surface Please make sure that no obstacles block ventilation To set up the ORIGINS by Elchrom chose an even clean and stable ventilation intakes located at the bottom plate of the apparatus ORIGINS by Elchrom Technical Manual 11 2012 Page 7 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 2 3 Safety instructions Always unplug the ORIGINS by Elchrom when the unit is not in use We do not warranty products against unauthorised modifications improper maintenance or repair work Repair work which is not carried out by a suitably qualified professional not following the manufacturer s instructions and not in accordance with local and national safety regulations will result in a loss of warranty rights and may be dangerous In such case the manufacturer s liability is excluded If you have any questions please contact the manufacturer Protect the power cord from mechanical damage Should any inexplicable or unexpected problems occur during the initial set up unplug the unit immediately and contact Elchrom Scientific and our local representative 2 4 Installation The following instructions will guarantee safe operation of the ORIGINS by Elchrom and minimise maintenance of the unit Work sp
16. chapter 3 4 Depending on the application and gel type different temperature settings will be used during the run The following table indicates the pump delay applied for each situation ORIGINS by Elchrom Technical Manual 11 2012 Page 16 of 31 P Elchrom Scientific Excellence in Electrophoresis www elchrom com Gel type Voltage Pump delay Spreadex gels 120 V 55 C X Spreadex gels 120 V 20 C X Poly NAT gels 120 V 55 C X 6 Poly NAT gels 120 V 20 C X PCR CheckIT gels 120 V 20 C X PCR CheckIT EB gels 120 V 20 C GMA 72 84V lt 10 C X Agarose gels lt 2 5 gt 84 V 20 C X Agarose gels gt 2 5 lt 84 V 20 C X 12 cm distance between electrodes 4 5 Running ready to use gels 1 Gel positioning After pre warming see 4 1 turn off the pump and position the gel in the gel compartment of the ORIGINS by Elchrom To facilitate gel handling special forceps are provided Use those to grab the gel by the 2 mm overhang of the plastic backing Make sure no air bubbles are trapped under the plastic backing or in the sample wells The ORIGINS by Elchrom gel compartment provides enough space to run 1 Wide Mini presentation or 4 Mini gels 8 samples each simultaneously 2 Catamaran 1 Chose the appropriate Catamaran for your gel type and format Use the Catamaran to position the gel in the centre of the gel compartment 2 Position one side of the Catamaran on the side
17. ctrophoresis www elchrom com ete Product Number Product description 74 2100 101 Electrode carriage support with Platinum wire anode red 8 2100 501 Power supply black cable 9 2100 502 Power supply red cable 2100 201 Pump for buffer circulation 230 V 2100 202 Pump for buffer circulation 150 V 2100 203 Temperature probe Platinum wires must be changed by Elchrom Scientific personal only AVA To order spare parts please state the product number product gt EN description and serial number see 3 2 of the ORIGINS by Elchrom in your order ORIGINS by Elchrom Technical Manual 11 2012 Page 28 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 9 Related Products Product Product description Number 2029CH PSE Electrophoresis Power Supply 200 V 2000 mA 230 V CH plug 2029E PSE Electrophoresis Power Supply 200 V 2000 mA 230 V EU plug 2029U PSE Electrophoresis Power Supply 200 V 2000 mA 115 V US plug 2350 Bind ET Ethidium Bromide removal system including cartridge ORIGINS by Elchrom Technical Manual 11 2012 Page 29 of 31 P Elchrom Scientific Excellence in Electrophoresis www elchrom com 10 Terms and Conditions Orders Orders can be placed by fax e mail or mail For orders and shipping from countries other than Switzerland please contact our local distributor Prices Terms All prices are based on delivery ex works Cham Switzerland Prices are subject to
18. cts should be handled only by qualified persons trained in laboratory safety procedures and familiar with the potential hazards In addition please read the product label and note the storage instructions and expiry date We are not liable for a product after its expiry date or for a product that has been misused or has become unusable due to improper storage or handling ORIGINS by Elchrom Technical Manual 11 2012 Page 30 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com Liability Our products are intended for laboratory and research use only and are not to be used for any other purposes We are not responsible for damage due to the misuse of our products Technical Assistance Elchrom Scientific AG provides high quality products and technical assistance and information We make no warranty regarding such technical assistance or information Applicable Law Swiss law For additional information on Elchrom s products please visit our web page ORIGINS by Elchrom Technical Manual 11 2012 Page 31 of 31
19. each room temperature RT 20 to 30 min 55 C Allow to reach RT 20 to 30 min first subsequently place gel onto Catamaran inside the pre warmed ORIGINS by Elchrom for approximately 30 minutes Note Each half of a Wide Mini gel can be used individually Separate the two halves by cutting through the gap between the two gels Each side should retain approximately 2 mm of plastic backing for fixation with the Catamaran The half to be run at a later time point should be re packed and re sealed in the plastic as well as the aluminium bag Close the latter tightly with tape and store the package at 4 C 40 F Properly sealed gels can be kept for up to 2 weeks ORIGINS by Elchrom Technical Manual 11 2012 Page 13 of 31 P Elchrom Scientific Excellence in Electrophoresis www elchrom com 4 2 Preparing TAE running buffer Elchrom s ready to use gels must be run in 1 x TAE running buffer Elchrom Scientific offers a standardised 40 x Stock Solution P N 3031 20 x 50 ml Preparing a 40 x TAE stock solution Tris hydroxymethyl aminomethane 145 37 g NazEDTA 2 H20 11 16 g Acetic Acid glacial 34 4 ml Dissolve Tris and NazEDTA in 800 ml of ddH20 In a fume hood add acetic acid and adjust to 1 litre with ddH20 For electrophoresis prepare 2 litres of 1 x running buffer by diluting 50 ml of the 40 x stock solution in 1 95 liters of ddH2O Fill the ORIGINS by Elchrom carefully with approximately 1 9 liters of 1 x
20. ightening Please contact us for details For any necessary repair work please inform our local representative and contact us directly Elchrom Scientific does not warranty products against unauthorised modifications improper maintenance or repair work Repair work which is not carried out by a suitably qualified professional not following the stor manufacturer s instructions and not in accordance with local and national safety regulations will result in a loss of warranty rights and may be dangerous In such case the manufacturer s liability is excluded If you have any questions please contact the manufacturer 7 2 Cleaning For cleaning always use ddH20 or follow the instructions provided in protocol 2 on our web page elchrom com protocols Do not use alcohols acetone or chlorinated solvents for cleaning ORIGINS by Elchrom Technical Manual 11 2012 Page 26 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 8 Spare Parts PEPPETTEEE ERED EEETEEEEE EEE ee m g pr a a Product Number Product description 1 2100 503 Flexible tube with Quick Fit for buffer emptying 2 2100 220 Cover 3 2100 152 Flow control barrier left 4 2100 100 Electrode carriage support with Platinum wire cathode black 5 Platinum wire 6 2100 151 Flow control barrier right ORIGINS by Elchrom Technical Manual 11 2012 Page 27 of 31 P Elchrom Scientific Excellence in Ele
21. ing volume is 4 8 ul Elchrom s ready to use gels are designed for standard pipette tips Wide Mini gels can be loaded with a multichannel pipette S 2x13 and S 4x13 formats follow the SBS 96 well standards the S 2x25 and S 4x25 formats the standards for 384 well plates 5 After loading close the cover of the ORIGINS by Elchrom 6 Check and adjust the values for voltage running time and pump delay if necessary Turn on the power supply and subsequently the timer of the ORIGINS by Elchrom ORIGINS by Elchrom Technical Manual 11 2012 Page 18 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com The pump delay controller will start the pump automatically after the pre set time N Zz Do not run gels in the ORIGINS by Elchrom without buffer NY circulation End of electrophoresis The timer of the power supply will end the electrophoresis run after the pre set time elapsed The ORIGINS by Elchrom will signal the end of the run by an acoustic alarm 8 Post staining 1 2 Y Lift the Catamaran and remove the gel by sliding it carefully against one side of the Catamaran Grab the plastic backing overhang with the special forceps and lift up the gel Alternatively use surgical injection needles to lift the plastic backing Transfer the gel onto the Peel IT and detach the gel with the provided nylon string Subsequently the gel is transferred into the Easy Stain
22. nstrations 25 6 1 Customer sample analysis 25 6 2 Demonstrations 25 7 Maintenance Repair and Cleaning 26 7 1 Maintenance and repair 26 7 2 Cleaning 26 8 Spare Parts 27 9 Related Products 29 10 Terms and Conditions 30 ORIGINS by Elchrom Technical Manual 11 2012 Page 3 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 1 General Information 1 1 Introduction ORIGINS by Elchrom Scientific is an advanced easy to use electrophoresis apparatus developed with the aim to maximise the reproducibility and resolving power of pre cast gels for DNA RNA electrophoresis ORIGINS by Elchrom Scientific features running buffer temperature control improved homogeneity of the electric field and laminar buffer circulation The combination of ORIGINS by Elchrom Scientific with Elchrom s ready to use gels provides unparalleled reproducibility resolving power throughput speed and convenience The ability to tightly control all relevant electrophoresis parameters makes the ORIGINS by Elchrom Scientific ideally suited to introduce new standards in high throughput routine and industrial electrophoresis applications Elchrom Scientific s products are intended for research use only 1 2 Symbols Danger stor High risk for personal injury Please follow the instructions provided carefully Warning Please follow the instructions carefully to avoid damages to equipment and or accessories
23. o slide the gel on the transilluminator and back into the tray 1 Easy Stain Gel Tray is suitable to stain 1 Wide Mini gel or 2 to 4 Mini gels ORIGINS by Elchrom Technical Manual 11 2012 Page 24 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 6 Customer Sample Analysis Demonstrations 6 1 Customer sample analysis For interested new customers Elchrom Scientific offers a sample analysis service Hereby you have the opportunity to send us up to 10 samples from your lab for analysis Not only will this service allow you to get a relevant impression of the advantages of the ORIGINS by Elchrom system but it will also provide you with a detailed protocol tailored and optimised for those samples sent to us Please visit our web page elchrom com FREE sample testing for further details and contact us or our representative in your country 6 2 Demonstrations Are you interested in a product demonstration Our partners in your country are able to introduce the ORIGINS by Elchrom to you and provide you with demo equipment Please contact them or us for details ORIGINS by Elchrom Technical Manual 11 2012 Page 25 of 31 K Elchrom Scientific Excellence in Electrophoresis www elchrom com 7 Maintenance Repair and Cleaning 7 1 Maintenance and repair The ORIGINS by Elchrom does require very little maintenance Depending on usage electrodes may require periodical changing or re t
24. s at room temperature pump switched on For some running buffers e g MOPS formaldehyde overnight equilibration is recommended 4 3 Preparing DNA samples DNA concentration The optimal DNA concentration depends on the detection method and the intercalating dye for detection In the case of EtBr the amount of DNA per band should be at approximately 2 to 20 ng For SYBR Gold or SYBR Green we recommend to use 0 4 4 ng band Note Compared to self made agarose gels ready to use gels from Elchrom Scientific are substantially more sensitive Consequently the amount of DNA per lane and per band is considerably lower hence smaller quantities of DNA can be analysed For samples of unknown DNA concentration we recommend to initially run dilution series e g undiluted vs 10 x diluted Maximal loading volume per gel type Optimal loading volume 4 8ul Maximal loading volume Mini Gel 18 ul Wide Mini S 2x13 S 4x138 S 2x104 25 ul Wide Mini S 2x25 S 4x25 S 2x200 14 ul Loading buffer We strongly recommend to use 5 x loading buffer by Elchrom Scientific Each gel box contains enough loading buffer stock solution for the total amount of sample wells of that particular box Additional loading buffer can be ordered from Elchrom Scientific P N Product description 3033 Sample Loading Buffer 10 ml BPB ORIGINS by Elchrom Technical Manual 11 2012 Page 15 of 31 P Elchrom Scientific Excellence in Electrophore
25. sis www elchrom com P N Product description 3034 Sample Loading Buffer 50 ml BPB 3035 Sample Loading Buffer 10 ml 2 dyes BPB and XC 3036 Sample Loading Buffer 50 ml 2 dyes BPB and XC Ideally add 1 ul of 5 x loading buffer to 4 ul of sample load onto the gel Note High salt concentrations may lead to distorted bands Desalting or diluting the sample may be required Do not load more than 50 ng of DNA per band Overloading severely A impairs resolution gt SDS concentration higher than 0 01 impair resolution To bypass post staining for PCR CheckIT gels we recommend to supplement the 1 x TAE running buffer with 0 5 ug ml of EtBr to avoid dye depletion during the run Do not add EtBr to the sample h an 4 4 Preparing the ORIGINS by Elchrom 1 Prepare 1 x TAE running buffer see 4 2 and use 1 9 litres to fill the electrophoresis chamber SE Do not turn on the ORIGINS by Elchrom without running buffer we S Do not turn on the pump without running buffer 2 Connect the ORIGINS by Elchrom to the power supply Power supplies from other suppliers my require special adapters 3 Switch on the ORIGINS by Elchrom The pump will turn on by default The display shows the current running buffer temperature and will heat or cool according to the pre set value By default the instrument stores the values form the previous run For different parameters re program the unit accordingly See
26. www elchrom com Combs 20 wells 1 5 mm thick 5 mm wide P N 2046 13 wells 1 5 mm thick 7 3 mm wide P N 2048 26 wells 1 5 mm thick 3 5 mm wide P N 2047 multi channel pipette compatible In the ORIGINS by Elchrom 2 wide mini gel trays can be run simultaneously 5 3 Special forceps To facilitate and ensure safe gel handling all manipulations are done with special forceps Sharp teeth at their tip guarantee a safe grip of the overhang of the plastic backing The gels are covalently linked to the plastic backing which confers physical stability on the gels and protects them from damages 5 4 Peel IT For post staining ready to use gels are best detached from the plastic backing This will increase diffusion of the dye significantly and therefore lead to more efficient staining Peel IT has been designed to easily detach the gel matrix from the plastic backing Peeling is mediated purely by physical detachment of the gel via a nylon string ORIGINS by Elchrom Technical Manual 11 2012 Page 23 of 31 0 Elchrom Scientific Excellence in Electrophoresis www elchrom com 5 5 Easy Stain Gel Tray The Easy Stain Gel Tray has been specifically developed to post stain Elchrom s ready to use gels After peeling the gels are relatively fragile and the Easy Stain Gel Tray allows hands free staining without the risk of breaking the gel A special grid A allows easy buffer exchange and a scoop B t
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