tacoTM Instrument user manual
Contents
1. eS ty 1 i tac MINI Research Use Only Automatic Nucleic Acid Extraction System User Manual Manufacturer GeneReach Biotechnology Corporation TEL 886 4 2463 9869 Email sales genereach com No 19 Keyuan 2 d Road Central Taiwan Science Park Taichung City 407 Taiwan Website www tacomag com Cat no atcd 8 2014 09 M taco M mini Automatic Nucleic Acid Extraction System Content vidc 1 T InIEOQUCIIOU condat bibens ovr ERE IEEE EUR OE E VENUE OE E E OE eg 2 INNEN e 2 1 2 taco mini Extraction Principle assess ccc csaiscnataneiasnsucweuniauebacau sda ats appe veas ev E 2 P CACO A E OU m E A E E A E 3 2 1 Product C OMpPONCINS doc casussasteccsccscasssadangiccdssasuennsenaceashaitabnedan EENE S EEEE 3 22 taco M mini SPC CIIC AION S sn aa a end beet uM Ea a EEA E 3 2 9 SOUIPMEHIIO Be S pplied Dy USET veteciscsntaccnedicesacesetarennstnnsawesabevens Seesausaeraceenadaeeacesetancnooues 3 2A Foncion DCP iee E R E E EE E 4 Dy Important NOLOS raa E T E EEE EOS 7 IL ca a a O Umm 7 eZ NC IUE 7 4 Operation Procedure OF taco mini scscssssssececccccsssscceecccsssssscccoccosssssccecocosssssseccesosssssseeeesesssss 8 4 1 How to START taco nil perta ehoc cicius lonctbsinsesDlanetanessnuseasianalgncthosaadlexedrexetuces 8 A42 Howto PAUSE taco miii eror erro2. 120V 200 240V AC 50 60 Hz 2A T 2 3 Equipment to Be Supplied by User taco Nucleic Acid Extraction Kit taco Plate amp Comb Uninterrupted power system UPS optional Laminar air flow optional Disposable gloves Protective goggles Lab coat Note To avoid power failure connecting taco mini to an UPS is recommended Note Use non recommended consumables may cause damage to the instrument taco M mini Automatic Nucleic Acid Extraction System 2 4 Functional Description 2 4 1 Front view of taco M mini Description Press to operate taco mini To protect interior mechanical components and operator Press to lock unlock the door To protect mechanical components and operator Note Make sure the top cover is in position when closing the door otherwise the door release may be damaged 2 4 3 Rear view of taco mini Ooo To turn on off the main power Cooling vents Ventilation M taco mini Automatic Nucleic Acid Extraction System 2 4 3 Internal view of taco mini Mixing Comb Binding of magnetic beads Mixing Comb holder Housing for the Mixing Comb Plate holder Housing for the 48 Well Extraction Plate taco mini Automatic Nucleic Acid Extraction System 2 4 4 Indicator description tac mini OVA LY m Signal Indicators During self testing E uu Remove used Mixing Comb Mixing Comb from l l Press operation button to proceed previous
3. nen Dori E E E 9 AS HOW t RESET faco i RC 9 44 TOW tosSHUTDOWN taco MiNi eun totes esses cc beataseaneeittectee te ysn Peta teu ii eoibet ated mee 9 S5 Cleanin and PVE ATIC ANN CO 3 E coctectecesssvncecesececessseceecessecosceusevenccesucrcesssuseesersacvecsusceseuceeesses 11 NMEGTI CI 87 EeE s P 11 2 2 Deano o HOS DIIS 15 oo ace cuese Sox austin us bu MES NOmUSMN SDUL DUDEN TM RMURUSd De UE UIS 11 o Tron pes hooo oe sstcceccessesecesssccecsusscssaexetoccesessessceurscceveusuaesscursaccuesssassscurcocesesssassscunsoccaestiscusssveceeess 12 M taco M mini Automatic Nucleic Acid Extraction System Symbols Date of manufacturing Manufacturer Catalog number Serial number Alternating current Consult instructions for use Caution consult accompanying documents This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE WEEE symbol Directive 2002 96 EC Follow your institutional requirements for disposal of the accessories Stop of action Start of action Door open Mixing Comb signal 4NCO a PE BIER taco M mini Automatic Nucleic Acid Extraction System 1 Introduction The taco mini Nucleic Acid Automatic Extraction System taco mini is a magnetic bead based nucleic acid extraction and purification device Nucleic acids bind to silica coated m
4. run has not l to self testing been removed l l Close the door and press the During self testing the l l operation button to proceed to door is open l self testing Mixing Comb is not l Insert the Mixing Comb inserted During extraction the door is open Please consult local distributor or System error l GeneReach for instructions Please refer to Procedure 4 2 for Extraction paused instructions EE Please refer to Procedure 4 3 or Extraction finished l l 4 4 for instructions 1O Signal light flashing Signal steadily illuminated The alarm will be on while door is open during operation Close the door to turn off the alarm and door signal light M taco M mini Automatic Nucleic Acid Extraction System 3 Important Notes After receiving taco mini check the instrument and accessories for any damages Should any questions arise contact your local distributor or GeneReach immediately Any damaged components may lead to poor performance of the instrument 3 1 Safety Information Not following the instructions may cause personal injury or device failure Place taco mini in a room with good ventilation Operation temperature range Ambient temperature Make sure the working area is clean dry flat and vibration proof leave enough room for cables lids and other accessories Keep power cord away from any hot surface Do not touch any plugs and elect
5. to stop taco M mini if necessary Flashing Q signal indicates the device is in the pause mode The comb holder will move up to the top To move comb holder up or down press the operation button while the door is open 4 2 2 To resume extraction close the door of taco mini first then press the operation button 4 2 3 To abort extraction reset the program see Procedure 4 3 or turn off the main switch Note Under the circumstances listed below the device does not go to the pause mode Drying of magnetic beads Transferring of magnetic beads 4 3 How to RESET taco mini 4 3 1 Users may reset taco mini when extraction is finished or in pause mode 4 3 2 Remove any 48 Well Extraction Plate and Mixing Comb 4 3 3 To reset taco mini close the door and press the operation button for 5 seconds in pause mode The system will start self test which should complete in 1 minute 4 4 How to SHUTDOWN taco M mini 4 4 1 Before switching off taco mini make sure 48 Well Extraction Plate and Mixing Comb are removed from the device Note At this point the magnetic rod module should be positioned above well 4 of 48 Well Extraction Plate Contact Genereach when the magnetic rod module is found to be mispositioned 4 4 2 Close the door of taco mini Turn off the main switch to shutdown taco mini M taco M mini Automatic Nucleic Acid Extraction System Note Transfer the extracted nucleic acids
6. agnetic beads allowing easy separation of nucleic acids from biochemical contaminants in the solution through automatic transfer and processing of magnetic beads Purified nucleic acids could be eluted from the beads after several washing steps 1 1 Intended Use taco mini is developed to work with nucleic acid extraction kits developed by GeneReach Biotechnology Corporation GeneReach to extract nucleic acids from samples for research purpose This product is intended for professional use by properly trained laboratory technicians familiar with molecular biology techniques 1 2 taco mini Extraction Principle taco mini based on the magnetic separation technology for nucleic acid extraction works with the specially designed disposable taco Plate amp Comb provided in all GeneReach nucleic acid extraction kits for taco mini The automated steps performed by taco mini include Transfer of magnetic beads Mixing magnetic beads with samples Sample lysis Binding of nucleic acids to magnetic beads Washing to remove unwanted biochemicals from magnetic beads Elution of nucleic acids from magnetic beads M taco M mini Automatic Nucleic Acid Extraction System 2 taco mini Layout 2 1 Product Components Component Quantity 1 taco M mini Power Cord User Manual Warranty Card 2 2 taco mini Specifications l i WxDxH 1260 mm x 265 mm x 300 mm Net Weight 5 5 kg Power Requirement 100
7. ion in each well does not exceed 900 ul before extraction 4 1 3 Open the door of taco mini by pressing the door release Insert the Mixing Comb into the Mixing Comb holder see illustration below The MM signal will go off 4 1 4 Press the operation button to raise the Mixing Comb Insert loaded 48 Well Extraction Plate from Procedure 4 1 1 into the plate holder Ensure the 48 Well Extraction Plate is fully inserted into the plate holder with the notched corner positioned at the far right 4 1 5 Close the door of taco mini press the operation button to start the extraction During the extraction step TACO min signal light stays illuminated a Power on and self test begin b Insert the Mixing Comb and Ill signal light will be on push it all the way into the Mixing Comb holder raise the Mixing Comb holder Extraction Plate and push it all the way in taco M mini Automatic Nucleic Acid Extraction System Note Operate taco mini only within its operation temperature range ambient temperature Note Open the door of taco mini during operation may cause alarm Please do not open the door during operation or pause the extraction before open it Note Under the circumstances listed below the extraction will not be activated Mixing Comb is not inserted The door of taco mini is open 4 2 How to PAUSE taco mini 4 2 1 During extraction press the operation button
8. ises from the machine d Liquids spill and adhered to the device Magnetic rods function failures a Stains on the magnetic rods b The magnet detaches from the magnetic rod e Make sure the plug is connected to the power system e Make sure the main power is switched on e Check voltage of electricity outlet see 2 2 taco mini Specifications e Contact your local distributors or GeneReach for assistance e Contact your local distributors or GeneReach for assistance e Make sure the Mixing Comb is pushed all the way into the Mixing Comb holder e Make sure the 48 Well Extraction Plate is pushed all the way into the plate holder e Make sure nothing prevents the track from moving e Contact your local distributor or GeneReach e The total volume of solution in each well should not exceed 900 ul e Repeat extraction procedures with new samples and reagents e Ensure the Mixing Comb is installed securely before each extraction e Contact your local distributor or GeneReach e Contact your local distributor or GeneReach 2014 GeneReach Biotechnology Corporation For research use only Not intended for any animal or human therapeutic or diagnostic use All rights reserved 12
9. ric switches with wet hands Keep taco mini away from water Do not unplug by pulling the power cord Hold the plug not the cord to unplug Turn off and unplug the machine before moving it Package taco mini carefully before moving and or delivery Vibrations or crashes may lead to device damage Users should revalidate taco mini after the device has been moved Waste disposal must be in compliance with local laws When handling infectious materials always wear a lab coat disposable gloves and protective goggles Do not repair the machine yourself Contact your local distributor or GeneReach directly to deal with the problems 3 2 Emergency Stop 3 2 1 Should any emergency occur press the operation button or turn off the MAIN SWITCH to stop taco mini 3 2 2 After all emergency is cleared follow the instruction to resume restart taco mini see 4 1 and 4 2 M taco M mini Automatic Nucleic Acid Extraction System 4 Operation Procedure of taco mini Note Make sure the top cover is in position when closing the door otherwise the door release may be damaged 4 1 How to START taco mini 4 1 1 Plug in taco mini and turn on the main switch The device will complete self test within minute 4 1 2 Load sample and reagents to 48 Well Extraction Plate in advance see taco nucleic acid extraction kit manuals for instruction Note Make sure total volume of the solut
10. to a new tube immediately after extraction Quality of nucleic acids may decline otherwise The extracted nucleic acids are ready for downstream applications Store at 80 C for long term storage 10 taco mini Automatic Nucleic Acid Extraction System 5 Cleaning and Maintenance Note Make sure the top cover is in position when closing the door otherwise the door latch may be damaged 5 1 General Care Do not disassemble taco mini for cleaning This will void the warranty Never allow liquids to sit on any surfaces of the device for extended periods of time Note Wear gloves or other protective gears Always turn off and unplug taco mini before cleaning 5 2 Dealing with Spills Do not use organic solvents except for 70 Ethanol detergents or bleaches to clean the internal surfaces Wipe off any visible spills with wet paper towel immediately Incase of serious liquid spills contact your local distributor or GeneReach for assistance Do not dismantle the instrument for cleaning Make sure total volume of the solution in each well does not exceed 900 ul before extraction Use UV light for sterilization after extraction for 30 minutes Open top cover for cleaning 11 Troubleshooting taco mini Automatic Nucleic Acid Extraction System Comments and Suggestions taco mini does not perform normally a No LED light signals b Self test failure c Unusual no
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