ÄKTAexplorer 10 to ÄKTAexplorer 100, Upgrade Kit
Contents
1. 8 Check the id no on the INV 907 valve 56 3047 07 It should be set to 7 The digit 7 should also be attached on the valve front plate to identify the valve 9 Use bracket 1 56 3002 99 locate and mount the valve V7 according to the diagram 56 3011 92 Use two M4 screws 59 3464 00 Note If the valve door is of the old type i e not moulded use bracket 6 56 3060 31 instead of bracket 1 10 Check the id no on the PV 908 valve 56 1196 08 It should be set to 5 The digit 5 should also be attached on the valve rear plate to identify the valve 11 Remove the lid covering the hole for the Sample valve V5 in the valve door Use angled bracket 2 56 3003 01 locate and mount the valve V5 according to the diagram 56 3011 92 Use two M4 screws 59 3464 00 AKTAexplorer 10 to AKTAexplorer 100 upgrade kit 4 Instruction 56 3060 21 Edition AA Last edited 1997 10 14 by G Goldring 12 13 14 Amersham Biosciences Locate and mount the Sample pump P 910 56 6500 00 according to the diagram 56 301 1 92 Use two M4 screws 59 3464 00 See Instructions P 910 included in the AKTAdesign Component binder on how to prepare the pump for operation Mount the 2 mm UV flow cell 18 1111 10 Use the fibre detachment tool 18 1111 16 Slide the rubber sleeves on the two optical fibres onto the connectors Make sure that the sleeves are pushed tight to the housing to prevent dust or fluid from entering the connections Note2. 15 Removing AKTAexplorer 10 components WARNING Ensure that the mains supply is disconnected before starting the installation WARNING Ensure that the entire system has been flushed thoroughly with distilled water before removing any components 1 Remove the 10 mm UV flow cell Use the fibre detachment tool 18 1111 16 2 Remove the four 10 ml pump heads Use the 3 mm Allen key included in Tool kit 56 3008 20 and save the attachment screws They will be re used AKTAexplorer 10 to AKTAexplorer 100 upgrade kit Instruction 56 3060 21 Edition AA Last edited 1997 10 14 by G Goldring Amersham Biosciences Installing AKTAexplorer 100 components 1 Assemble the 100 ml pump heads according to instructions in the Pump P 900 User Manual Changing piston sealings 2 Mount the 100 ml pump heads Please observe the UP marking Fit in position and fasten using the old attachment screws 3 Mount the piston seal rinsing system capillaries Please observe the flow direction of the check valve arrow should point towards the front of P 900 4 Mount the purge capillaries 5 Mount the pump head capillaries The capillaries from the A pump heads should be connected to the inner part of the outlet manifold 6 Mount the inlet manifolds on each pump module and fix with the screwed end pieces do not overtighten 7 Attach the Switch valves SV 903 to the mounting rail on P 900 Check that the control cable is running free
3. AKTAexplorer 10 equipment for possible future use Software pre requisites Before you start disassembling the AKTAexplorer 10 system install the new software included in the conversion kit General installation instructions for UNICORN software are given in the UNICORN User Manual Chapter 13 Remember to check the boxes for loading strategy and templates Required tools and documents Tools included in Tool kit 56 3008 20 included in existing system Fibre detachment tool 18 1111 16 included in existing system Connection diagram UniNet 2 56 3011 92 included in upgrade kit Connection diagram capillaries 56 3011 93 included in upgrade kit AKTAdesign Component binder 18 1120 17 included in existing system AKTAexplorer 10 to AKTAexplorer 100 upgrade kit Instruction 56 3060 21 Edition AA Last edited 1997 10 14 by G Goldring Amersham Biosciences Removing AKTAexplorer 10 capillaries WARNING Ensure that the mains supply is disconnected before starting the installation WARNING Ensure that the entire system has been flushed thoroughly with distilled water before removing any capillaries Remove all capillaries and connectors from the separation unit and the Pump P 903 The following items will be reused e WI Tefzel 0 75 i d L 1300 mm e W2 Tefzel 0 75 i d L 1200 mm e W3 56 3047 57 e Piston seal rinsing system 18 1113 32 e Purge kit 18 1115 63 e Flow restrictor FR 904 18 1112 42 e Inlet filters 18 1113
4. edited 1997 10 14 by G Goldring 10 Amersham Biosciences Cut off the connector from W2 capillary and thread it through the hole under V5 from the inside Check that the capillary designation is on the inside of the valve door and connect W2 Put the open end of W2 in a waste flask The cut off connector can be disposed of 11 Connect W1 to V1 and put the open end of W1 in a waste flask 12 Connect G17 between V1 and V5 13 Thread the G5 capillary through the hole under V1 from the outside Check that the capillary designation is on the inside of the valve door and connect GS 14 Thread the G6 capillary through the hole under V2 from the outside Check that the capillary designation is on the inside of the valve door and connect G6 15 Thread the G7 capillary through the holes under V2 and V3 from the inside Check that the capillary designation is on the inside of the valve door and connect G7 16 Thread the G8 capillary through the hole under V3 from the outside Check that the capillary designation is on the inside of the valve door and connect G8 17 Connect a stop plug in V7 port 5 18 1112 52 18 Mount G9 on V7 19 Connect G10 to the inlet of the UV flow cell top connection and V7 20 Connect G11 from the outlet of the UV flow cell bottom connection to the conductivity flow cell The flow direction of the flow cell should be so that the screw head end of the flow cell faces the pH flow cell 21 Thread the
5. Amersham Biosciences AKTAexplorer 10 to AKTAexplorer 100 Upgrade kit Instruction Conversion kit code number 18 1125 16 IMPORTANT Read through this instruction in whole before commencing with the installation IMPORTANT Install the new software included in this kit before switching off the existing system Contents GENERAD sees se naen E ees sae 65 505 0a bus apa suc eaaea Vek so ue sae Sagwea due E aaa sabes E dR TNaEUbEca see EE RE 1 1 OA 27 0 lt 4 1N Cpe 2 SOFTWARE PRE REQUISITES os cc sacs Gieesd coke dated dc a 2s eeu che AE E A ance sao AE E E E ole AR at uess 2 REQUIRED TOOLS AND DOCUMENTS sccccssssscccssscececsseeecesssececscsececsseeecesssececsseeceessseeceesaeecenseseceesseeeesesaeeeenseeeeees 2 REMOVING AKTAEXPLORER 10 CAPILLARIES csceccscecssessssecessscesescsvsceececsessavsceesecaessavsceesecsessvavacessecsesavavaceseessents 3 REMOVING AKTAEXPLORER 10 COMPONENTS cccccscssessssecescscssscsvsceesecsessavsceesscsessavscessecsessvavacessecsesavavaceseessenes 3 INSTALLING AKTAEXPLORER 100 COMPONENTS sccssessssecescscesesesveceececssescavscsesecssescavsceesecsessvavacessecsessvavaeeseevsents 4 INSTALLING AKTAEXPLORER 100 UNINET 2 CABLES uc ccccccsccssesessecescecssssesvsceesecessscavsceececsessvavacessecsensvavacescessents 5 INSTALLING AKTAEXPLORER 100 CAPILLARIES s c cecssessssecescscssesesvsceececsssscavsccesecsessavsceesecaessvavacessscsensvavsseseevsents 5 CHECKING THE INSTALLATION ccccccecsss
6. Fit all the protective caps on the old 10 mm flow cell If the pH flow cell included with the original KTAexplorer 10 delivery is not fitted it should be mounted according to the instructions in the KTAexplorer 10 System Manual Section A 6 Installing KTAexplorer 100 UniNet 2 cables 1 Install the UniNet 2 cables according to diagram 56 3011 92 Installing AKTAexplorer 100 capillaries 1 If not already done unpack and check the AKTAexplorer 100 tubing kit cpl 18 1126 30 2 Change the inlet filter inserts 18 1114 42 if required 3 Attach the inlet filters to capillaries A11 A2 B1 and B2 4 Connect the inlet capillaries A1 A3 A11 and B1 B3 in that order according to diagram 56 3011 93 5 Connect G1 and G2 to the 2 ml mixer chamber inlets G1 is connected to the inner connection of the outlet manifold A pump module 6 Attach the on line filter 18 1112 44 to the G3 capillary and connect to the mixer outlet Observe the flow direction marked by an arrow 7 Thread the G4 capillary through the hole under V1 from the outside Check that the capillary designation is on the inside of the valve door and connect G4 8 Mount the sample loop 500 ul 18 1113 99 on V1 9 Thread the G18 capillary through the hole under V1 from the inside Check that the capillary designation is on the inside of the valve door and connect G18 AKTAexplorer 10 to AKTAexplorer 100 upgrade kit 5 Instruction 56 3060 21 Edition AA Last
7. G12 capillary through the hole under V4 from the inside Connect G12 between the conductivity flow cell and the lower connection of the pH flow cell marked IN 22 Attach the flow restrictor FR 904 marked IN to the G13 capillary and connect to the pH flow cell upper connection marked OUT The flow restrictor should be housed in the hole in the pH flow cell holder store the dummy pH electrode in Box 900 when not used 23 Connect G14 24 Connect W3 Put the open end of W3 in a waste flask 25 Connect G15 The connection of G15 to the tube holder on the Frac 901 delivery arm is described in the Frac 900 901 User Manual 26 Connect F3 Put the open end of F3 in a flowthrough container AKTAexplorer 10 to AKTAexplorer 100 upgrade kit 6 Instruction 56 3060 21 Edition AA Last edited 1997 10 14 by G Goldring Amersham Biosciences 27 Mount the sample holder parts 56 3033 37 on the plastic mounting plate below V5 Install the parts sample tray tube holders and tubing holder by pushing them into the slits Checking the installation When all of the installation is carried out check that all the connections and mountings are performed according to the diagrams 56 3011 92 and 56 301 1 93 All excessive material is stored in Box 900 AKTAexplorer 100 installation test Perform an installation test as described in the AKTAexplorer 100 Installation Guide Chapter 6 AKTAexplorer 100 test record Make a photocopy of the t
8. ceceessecccesscececsseeecesssececnsssececaeeccesssececeesseceesseeecsesseeccnseseceesseeeeseaaeeeenseaeeees 7 AKTAEXPLORER 100 INSTALLATION TEST c cccsceccssecssesssccescscssescavsccesecsssscavsceevecaessavsceesecsessvavacessecsensvavaseseesaeets 7 AKTAEXPLORER 100 TEST RECORD sccccscecesesessecescecsseseavsccevscssssavscsesscasssvavsceesecaessavsceesecsessvavaseesecsessvavsceseecsenes 7 ACCESSORIES 355523 csces sess ccs dgees E nea E sana sacens Sesudusbeeseesueeeeueusouesaeenasusueeseerseossauncadenuuesvssuseaccceauvesaasaacones 7 General The AKTAexplorer 10 to AKTAexplorer 100 upgrade kit converts the flow rate from 10 ml to 100 ml and maximum pressure range from 25 MPa to 10 MPa P903 is converted to P 901 It adds new functions such as automatic sample application P 910 and PV 908 and reversed flow through column INV 907 The kit also includes new software and new manuals as well as capillaries and other components in the flowpath Amersham e KY e Biosciences Edition AA Last edited 1997 10 14 by G Goldring Amersham Biosciences Unpacking Unpack the AKTAexplorer 10 to AKTAexplorer 100 conversion kit and check the items against the supplied packing list Inspect the items for obvious damage which may have occurred during transportation Please note that some of the included packages have their own packing lists included Check these as you go along It is recommended to reuse the packing material to pack the surplus
9. est record included in the AKTAexplorer 100 Installation Guide Chapter 7 and store the test record in the AKTAdesign System Logbook Store also the packing lists in the System Logbook Accessories The accessories included in the upgrade kit is to complement the upgraded system to original delivery status Store the accessories in Box 900 or wherever convenient for the customer AKTAexplorer 10 to AKTAexplorer 100 upgrade kit 7 Instruction 56 3060 21 Edition AA Last edited 1997 10 14 by G Goldring
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