Sample Concentrator
Contents
1. Sample Concentrator OPERATOR S MANUAL Bibby Scientific art ome ome CONTENTS PAGE INTRODUCTION 2 Warning 2 Operator safety 3 Specification 3 Installation 3 Diagram of Sample Concentrator 4 Guarantee 5 OPERATION 6 Preparation 7 Operation 8 ADDITIONAL INFORMATION 9 User maintenance 9 Solvent evaporation temperatures 10 Diagram of gas chamber 12 Replacement parts 13 ome INTRODUCTION Please read all the information in this user manual before using the Sample Concentrator The Techne Sample Concentrator has been designed to provide a quick and convenient method of accelerating the concentration by evaporation of solvents from samples prior to analysis It is designed to work in conjunction with Techne DB 3 DB 3A or DB 3D Dri Block heaters The Sample Concentrator consists of a gas chamber mounted above a Dri Block heater into which vessels containing samples are placed Hyperdermic needles carry the gas down from the chamber into he test tubes The samples are heated from below by the heater and the flow of gas directed over the surface of the samples displaces the evaporated solvent from above the liquid s surface This significantly increases the rate of sample concentration The unit provides accurate and reproducible control over the positioning of the needles The gas chamber can be removed from its stand to provide easy access to the samples and it can be replaced q2. cientific com Bibby Scientific Italia Srl Via Alcide de Gasperi 56 20077 Riozzo di Cerro al Lambro Milano Italia Tel 39 0 2 98230679 Fax 39 0 2 98230211 e mail marketing bibby scientific it www bibby scientific it
3. e Ethylene chloride 83 210 1 2 dichloroethene Cis acetylene dichloride 60 479 1 2 dichloroethene Trans acetylene dichloride 48 751 2 propan 2 yloxypropane Isopropyl ether 68 375 1 4 Dioxane Diethylene oxide 101 107 N N dimethylformamide Dimethylformamide DMF 153 11 Acetic acid Ethanoic acid 118 44 Ethanol Alcohol 79 175 Ethyl acetate Ethyl ester 77 40 Heptane Dipropylmethane 98 120 Hexane n hexane 69 335 Propan 2 ol Isopropanol 82 137 3 methyl 1 butanol Isoamyl alcohol 130 14 Butan 2 one Methylethylketone MEK 80 243 10 some SOLVENT EVAPORATION TEMPERATURES continued IUPAC Name Common Name Boiling point C at Pressure mbar at 1013 mbar pressure which b p is 40 C Methanol Methyl alcohol 65 337 Dichloromethane Methylene chloride 40 Atmospheric Pentane Pentane 36 Atmospheric Propan 1 ol Propyl alcohol 97 67 1 1 1 2 2 pentachloroethane Pentachloroethane 162 13 1 1 2 2 tetrachloroethane Tetrachloroethane 138 35 1 1 1 trichloroethane Trichloroethane 75 271 Tetrachloromethane Carbon tetrachloride 76 300 1 1 2 2 tetrachloroethene Tetrachloroethylene 21 53 Oxolane Tetrahydrofuran THF 67 357 Toluene Methylbenzene 111 77 1 1 2 trichloroethene Trichloroethylene 87 183 Oxidane Water 00 72 Dimethylbenzene Xylene mixed 37 25 1 2 dimethylbenzene o xylene 144 34 1 3 dimethylbenzene m xylene 39 25 1 4 dimethylbenzene p xylene 38 31 11 ome DIAGRAM OF GAS CHAMBER Matrix plate Sealing plate Supp
4. e label amp number number 6mm x 30 F3502 A Omm x 20 F3503 B 2mm x 20 F3504 B 3mm x 20 F3505 B 15mm x 12 F3506 C amp D 16mmx 12 F3507 C amp D 9mm x 8 F3508 D E amp F 25mm x 6 F3509 D amp E 9mm x 10 F4461 A 7mm x 20 24mm x 6 F4462 D amp E 26mm x 6 F4463 D amp E 1 5ml x 20 F4464 B 0 5ml x 30 F4465 A ome A A A A eA oA A B B B B B B de nia ie ie ne Dos i p e As A o Ao eA 0 ee eA eA B B ef B B B B o o ec ec ec ec A A A A eA A eA o eB sE eB B sE eB eB eE eB eB sE eB eB eE eB eB eE eB o A A A A A A eA A A ec ec ec ec ec o B eB B op F oB B B B eA eA Ae eA eA eA eA oae eA A D o eepe ep D B B B eB C oB B B eB A 0A eA eA eA eA eA eA eA oA eA eA 96 well hole array 12 x 8 matrix as above Press each needle firmly pointed end first through the guide holes and through the sealing pad behind so that it is held firmly in position Build up the desired pattern of needles to suit the arrangement of test tubes to be used Do not put a needle in any position which will not have a corresponding sample to be concentrated as this will only waste gas The needles may be removed and replaced many times as the pad is self sealing INSERTING THE NEEDLES 4 Replace the gas chamber o
5. ld be obtained from the supplier Notwithstanding the description and specification s of the units contained in the user manual Techne hereby reserves the right to make such changes as it shall see fit to the units or to any component of the units This user s manual has been prepared solely for the convenience of Techne customers and nothing in this manual shall be taken as a warranty condition or representation concerning the description merchantability fitness for purpose or otherwise of the units or the components Preparation ome OPERATION 1 Place the aluminium insert blocks to be used into the Dri Block unit The blocks should touch each other and be positioned centrally 2 Loosen the locking knob on the carriage Remove the gas chamber disconnecting the gas pipe if necessary and lay the chamber upside down on a suitable work surface 3 Insert the hyperdermic needles in the pattern to suit the test tubes to be placed in the heater The needle guide holes are labelled with a letter corresponding with the type of insert block used in the heater These are listed in the following table As an example if you use the block for twelve 15mm test tubes block number F3506 you would place a needle in all the holes labelled C and all holes labelled D Only Sample Concentrators with the part number FSC496D or a replacement head part number 6104202 will have the 96 well array Tube size Insert block Hol
6. low for wear the friction pressure may be increased by tightening the 4 screws on the rear of the carriage sufficiently to give adequate grip Ensure that all four are tightened equally To replace the sealing pad After many repeated needle insertions the self sealing property of the sealing pad may start to degrade Spare pads can be obtained from your supplier or from Techne Replacing the pad is simply accomplished as follows Remove the gas chamber from the carriage disconnect the gas pipe and place upside down on a suitable work surface Remove the four nuts securing the matrix plate Lift off the matrix plate leaving the four spacers in position Lift off the sealing pad Ensure that the support strip underneath the sealing pad and the four spacers remain in place Replace the sealing pad with the new one Reassembly is the reverse of the above procedure For any other servicing requirements please contact your supplier or Techne s service department Fora SOLVENT EVAPORATION TEMPERATURES IUPAC Name Common Name Boiling point C at Pressure mbar at 1013 mbar pressure which b p is 40 C 2 propanone Acetone 56 556 1 pentanol Pentyl alcohol 137 11 Benzene Benzene 80 236 1 butanol Butyl alcohol 118 25 2 methyl 2 propanol Tert butyl alcohol 83 130 Chlorobenzene Chlorobenzene 132 36 Chloroform Trichloromethane 61 474 Cyclohexane Cyclohexane 81 235 Ethoxyethane Diethyl ether 35 Atmospheric 1 2 dichloroethan
7. nto the carriage and tighten the locking knob Re connect the gas pipe if necessary ome PROCESSING OF SAMPLES Warning remember high temperatures are dangerous The heater insert blocks sample vessels needles and samples may be very hot Always take the precautions listed earlier in this manual 1 By turning the carriage handle on the right hand side counter clockwise raise the gas chamber to take the needles well away from the heater Place the sample vessels in the insert blocks and lower the chamber so that the tips of the needles enter the test tubes Position them at the required height above the liquid surface Set the heater to the required temperature and adjust the gas flow to the desired rate Remember not to exceed 2 psi To increase the rate of concentration the chamber may be lowered as evaporation takes place so that the needles follow the level of the samples in the tubes Using the scale on the column fine adjustments are easily made On completion switch off the gas flow and raise the chamber out of the way For operation of the Dri Block see the user s manual supplied separately orm ADDITIONAL INFORMATION User maintenance 1 Cleaning Your Sample Concentrator can be cleaned by wiping with a cloth dipped in soapy water Ethanol and formaldehyde may also be used Do not use abrasive cleaners Carriage friction Height adjustment is by means of a friction drive To al
8. ort bracket already fixed to the base board Locate the column on the two pins in the support bracket Screw the fastening knob through the rear of the column and tighten firmly 2 Insert the locating pin on the rear of the gas chamber into the front of the carriage aligning the indent in the chamber with the positioning pin on the carriage Tighten the locking knob ome DIAGRAM OF SAMPLE CONCENTRATOR Positioning pin Carriage Locking knob Locating J ia Column Fastening knob Location spigots Baseboard Connect the intake nozzle at the rear of the gas chamber to a suitable supply of gas via a pressure reducing valve The gas pressure should be adjusted as required at the reducing valve but should not exceed 2 psi Pressure in excess of 2 psi will cause gas to be vented and wasted Place the Techne Dri Block heater onto the base of the Sample Concentrator It is positioned by means of two spigots on the base board which align with two centrally located screw heads on the underside of the Dri Block unit some GUARANTEE This instrument is guaranteed against any defect in material or workmanship for a period as specified on the enclosed guarantee card This period is from the date of purchase and within this period all defective parts will be replaced free of charge provided that the defect is not the result of misuse accident or negligence Servicing under this guarantee shou
9. ort strip Spacer 12 ome REPLACEMENT PARTS AND ACCESSORIES The following replacement parts and accessories may be obtained from your supplier or from Techne Part Number Description F7209 Pack of 100 x 76mm long needles F7210 Pack of 100 x 127mm long needles FSCANCS Pack of PTFE coated needles 100 x 76mm long FSCANCL Pack of PTFE coated needles 100 x 127mm long 6101609 Base board 6101608 Spigot 6101604 Sealing pad 6101606 Spacer 6101605 Support strip Spare and alternative heads complete 6010080 Standard three block matrix head 6104202 96 well holes added to standard matrix 6103824 96 well head off set for automatic machine Concentrators with various heads FSC400D Standard three block matrix FSC496D 96 well lay out added to standard matrix Can be used with standard blocks or 96 well block but is not easy to differentiate for needle insertion 13 Bibby Scientific Bibby Scientific Ltd Beacon Road Stone Staffordshire ST15 OSA United Kingdom Tel 44 0 8449 360234 Fax 44 0 8449 360235 email techne bibby scientific com www bibby scientific com Techne Inc 3 Terri Lane Suite 10 Burlington NJ 08016 USA Tel 800 225 9243 Fax 609 589 2571 email labproducts techneusa com www techneusa com Bibby Scientific France SAS ZI du Rocher Vert BP 79 77793 Nemours Cedex France Tel 33 1 64 45 13 13 Fax 33 1 64 45 13 00 e mail bsf bibby scientific fr www bibby s
10. uickly The whole unit is compact enough for convenient use in a fume cupboard WARNING HIGH TEMPERATURES ARE DANGEROUS they can cause serious burns and ignite combustible material Users should pay attention to the following points e USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS e DO NOT put hot objects on or near combustible objects e DO NOT place any liquid directly into your Dri Block heater e At all times USE COMMON SENSE The Dri Block heater is not provided and must be purchased separately OPERATOR SAFETY ome SAFETY ADVICE BEFORE USE All users of Techne equipment must have available the relevant literature needed to ensure their safety It is important that only suitably instructed personnel operate this equipment in accordance with the instructions contained in this manual and with proper safety standards and procedures SPECIFICATION Maximum vertical travel Maximum gas pressure Maximum gas usage Gas Needle positions Gas Intake Nozzle Diameter Height Width Depth Weight INSTALLATION 320 mm 2 psi 15 litres min Any inert gas often nitrogen Variable to suit Techne Dri Blocks 6 35 mm 1 4 530 mm 240 mm 295 mm 3 5 Kg For packing purposes the Sample Concentrator is supplied unassembled in three parts the base board the column and carriage and the gas chamber The unit is re assembled as follows 1 Unscrew the column fastening knob from the column supp
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