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High Force Modulat Pumping Component User`s Manual

1. Where lt message gt is one of the following Syntax error in a received command NA Command not applicable at this time OOR Control data is out of the operating range of the pump Note If assistance is needed in programming pump control via RS 232 call Harvard Apparatus Customer Service Support at 1 800 272 2775 Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix A Syringe Diameters in mm Size Stainless Steel Diameter Size Terumo Diameter 8 cc 20 50 100 200 9 525 mm 19 130 28 600 34 900 44 755 Becton Dickinson Plastic Plasti pak Diameter 4 78 mm 8 66 12 06 14 50 19 13 21 70 26 70 Air Tite All Plastic Diameter 9 60 mm 12 45 15 90 20 05 22 50 29 00 Unimetrics Series 4000 amp 5000 Publication 5415 001 REV C Diameter 0 460 mm 0 729 1 031 1 460 2 300 3 260 4 610 3 cc 5 10 20 30 60 8 95 mm 13 00 15 80 20 15 23 10 29 10 Sherwood Monoject Plastic Size 20 35 60 140 Diameter 4 65 mm 8 94 12 70 15 90 20 40 23 80 26 60 38 40 Popper amp Sons Inc Perfektun Glass Size 0 25 cc 0 5 Diameter 3 45 mm 3 45 4 50 8 92 8 99 11 70 14 70 19 58 22 70 29 00 35 70 Renfac Diameter 9 12 mm 12 34 14 55 19 86 23 20 27 60 SGE Scientific Glass Engineering Size 25 pl 50 100 250 500 1 0 ml 2 5 5 10
2. After each command is received and executed the pump acknowledges the com mand with a prompt Preceding the prompt may be some additional text responses The additional text will be one or more lines of ASCII text each preceded by a line feed and terminated by a carriage return lt If gt lt text gt lt cr gt A prompt is a string of ASCII characters sent by a pump indicating the pumps address and its present state lt If gt 1 or 2 digit address prompt character Prompt Characters EN Av gt Publication 5415 001 REV C Meaning Pump stopped Pump infusing Pump refilling Pause interval Pump stopped Pumping interrupted Pump stopped Dispense trigger wait Pump stopped 2 Harvard Apparatus High Force Modular Pumping Component User s Manual Pump Chain Commands Contd Pump Commands and Responses RUN STP DEL CLD RAT Starts pumping according to the present setting of the pump If pump is already pumping a Not Applicable response will be given Stops pump if it was running If pump was already stopped a Not Applicable response will be given Request for volume delivered in ml Response is of the following format space space f f f f f f Request to zero volume delivered If the pump was interrupted it will can cel the interrupted condition If the pump is running request will not be accepted and a Not Applicable response will be given Otherwise no respon
3. Although you can view and create programs for pumps with Symphony you can only run pump programs if your pump has program capability and you have downloaded the program to the pump NOTE Pump programs are not run within Symphony A program must be loaded into pump memory in order to run There are three different versions of PHD 2000 pumps Infuse only Infuse Withdraw and Programmable The program control function will only work with the Programmable version PHD 2000 pump Publication 5415 001 REV C Declaration of Conformity Application of Council Directive s Standard s to which conformity is declared Manufacturer s Name Manufacturer s Address Type of Equipment Model No 73 23 EEC EN61010 1 1993 A2 1995 Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 U S A Syringe Pump 70 2202 High Force Pump Module I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place Date CE HARVARD APPARATUS United States of America September 06 2002 A np 2 f Signature Beth Bauman Full Name VP Engineering Operations Position
4. Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Operation Figure 1 Syringe Loading Syringe Loading The syringe holder and pusher block are fitted with movable retaining brackets 8 amp 9 which hold firmly the syringe barrel and plunger when refilling When loading the syringe into the pump it is necessary to adjust these brackets The pusher block is fitted with a mechanism 1 to release the drive nut from the leadscrew so that the block can be moved freely to facilitate loading the syringe 2 Loosen the screws on the syringe block 3 and pusher block 2 to free the retaining brackets 8 amp 9 3 To free the pusher block from the leadscrew turn the knob on the front of the block 1 until the knob slips into the slots 4 The syringe clamp post s locking screw 4 is located on the right side of the syringe block amp should be loosened so that the syringe clamp post 7 can be rotated to the side 5 Place the syringe barrel on the syringe holder block and move the pusher block to accommodate the plunger 6 Make sure the syringe barrel flange and the plunger flange are held by the retaining brackets 8 amp 9 Press the retaining brackets firmly against the flanges and tighten the retaining screws Publication 5415 001 REV C 3 Harvard Apparatus High Force Modular Pumping Component User s Manual Operation Contd 7 Rotate the syringe clamp post 7 a
5. Control data that is changed via RS 232 will be stored in the pump s nonvolatile memory After each command is received and executed the pump terminates its responses with a prompt A prompt is a string of ASCII characters sent by a pump The High Force Modular Pumping Component is capable of using two sets of pump interface commands This will allow users of Harvard Apparatus Model 22 and Model 44 pumps to use existing programs to control the High Force Modular pumps The two sets of commands are contained on the following pages For customer reference the High Force Modular Pumping Component is factory set at the 44 Protocol Each command sent to the pump chain is a string of ASCII characters with leading zero s on numbers and all spaces optional Numbers are a maximum of five digits The following symbols are used in describing the commands Publication 5415 001 REV C 4 Harvard Apparatus High Force Modular Pumping Component User s Manual Pump Chain Commands Contd Pump Chain Commands Model 22 Protocol Model 44 Protocol on page 35 After each transmission to the Pump terminating with a CR character ASCII 13 the pump enters remote mode and responds with the three character sequence CR LF prompt The prompt character indicates the status of the pump as follows When stopped ASCII 58 decimal gt When running forward ASCII 62 decimal lt When running reverse ASCII 60 decimal When stalled
6. The following examples are laid out for inputing data thru the optional keypad This same data can be entered thru the RS 233 connection See pump chain com mands starting on page 27 Multiple Infusion Example The following program will instruct the pump to infuse according to the above graph The program instructs the pump to infuse 10 ml at 75 000 ml mn then infuse another 5 ml at 25 ml mn then stop for a total of three sequences Since this graph is Rate vs Volume Strategy 1 will be used RATE makrini SEQUENCE H when entering the program SEQUENCE 1 SEQUENCE 2 SEQUENCE 3 75 000 ml mn 10 000 ml INFUSE Key Presses VOLUME mil Figure 3 Multiple Infusions Explanation PROGRAM ENTER 75 INFUSE RATE ENTER 0 ENTER 10 ENTER INFUSE REFILL ENTER Key Presses Allows selection of Programming mode Press until PROFILE selected Enters selection Enter rate of 75 000 ml mn Press until units are ml mn Enters rate Enter 0 for the time this indicates Strategy 10 ml is the first target volume Toggles direction to infuse Enters sequence s pumping direction Explanation PROGRAM ENTER 25 INFUSE RATE ENTER 0 ENTER 5 ENTER INFUSE REFILL ENTER Key Presses Press until PROFILE selected Enters selection Enter rate of 25 000 ml mn Press until units are ml mn Enters rate Enter O for the time this indicates Strategy 5 ml is the second target volume Toggles direct
7. When the volume in the syringe reaches the set refill volume Auto Fill will stop and the previous operation of the pump will resume Auto Fill continues to monitor the volume of the syringe TTL direction output is toggled ON during refill Refill Rate defaults to Infuse Rate if not set Note Auto Fill will only activate while infusing G e if the pump direction is set to Refill the pump will not stop when the syringe is full Also if the syringe plunger is manually moved the pump will lose track of the true syringe volume Publication 5415 001 REV C 3 Harvard Apparatus High Force Modular Pumping Component User s Manual Program Mode Program Description A program is made up of a set of sequences Each sequence being a set of operating instructions for the pump to follow When the pump is started in the PROGRAM run mode the pump will start at sequence 1 and execute the operating instructions in that sequence When the pump has completed the instructions for a sequence it will go to the next or specified sequence and execute the instructions in that sequence The pump continues this process until it either has reached a STOP operation the pump is manually or remotely stopped or the last sequence has been completed 4 programs with up to 9 sequences each may be entered A sequence consists of a sequence number indicating the order of the sequence a mode indicating what operation the sequence will be performing a
8. far ran mi B C a ial p fi Carri Accor flod f ote Canoe fe mi This window allows you to set on the fly all pump parameters syringe type and diameter flow rates and modes pump volume program and auto fill Values can be directly entered into fields or scrolled using up and down spin controls The volume delivered bar indicates percent volume delivered From this window you gain access to all other Symphony windows via the pull down menus or the icon buttons Symphony vpc file format Publication 5415 001 REV C Custom configurations are saved and opened in the Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix M Symphony Manual contd 5 2 Pump Graph The pump graph is like a continuously running strip chart recorder This window allows you to monitor infuse and refill rates of your pump along with dispensed vol ume Chart colors have meaning Infuse is shown in blue and refill in red The vertical axis scale range and horizontal axis chart speed period are selec table Graphs may be viewed only in the graph window and cannot be saved The reset button clears the screen Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix M Symphony Manual Contd 5 3 Pump program screen EXAMH ti Pupan If you have any Harvard model 44 PHD4400 or a PHD 2000 programmable version pump you may use the Progr
9. fy this How to run Symphony with multiple pumps Three modes are available They are Mode 1 Use this mode if you want all pumps to perform the same tasks simultaneously Connect one comm port on your PC to all syringe pumps in daisy chain fashion Set all pumps to the same address ex 00 Remember that the baud rate must be the same for Symphony and all pumps in the chain In this mode all pumps will respond to the same commands from Symphony although a small delay will occur between the first and later pumps This delay results from the time required to pass serial commands from one pump to the next along the chain Use the faster baud rates to minimize this delay Publication 5415 001 REV C 2 Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix M Symphony Manual Contd Mode 2 Use this mode if you have a small number of pumps and you want them to do differ ent tasks Connect a single comm port on your PC to all syringe pumps chained together and set all pumps to different addresses ex 00 01 etc In this mode to address a specific pump you must make it active by setting its unique address via the pump interactive screen Remember that the baud rate must be the same for Symphony and all pumps in the chain When running in this mode the log sheet will only record the activities of the active pump The log sheet header records only the pump address of the active pum
10. 252 Operating Keypad 2400 235 LCD Display Publication 5415 001 REV C 3 Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix K Maintenance amp Troubleshooting Maintenance The High Force Modular Pumping Component series pumps require no special main tenance other than keeping them clean by avoiding accidental spills of pumped material The two guide rods and the lead screw should be sparingly lubricated periodically The guide rods and the lead screw should be lubricated with Magnalube G R grease Catalog 0606 086 provided with the pump Solvents of any type should never be used to clean the pump A mild detergent solution may be used to clean the keypad Troubleshooting Infusion Accuracy To ensure infusion accuracy always use new syringe s and measure syringe bore diameter and enter actual dimensions in millimeters mm Additionally make sure that the guide rods and lead screw are properly lubricated RS 232 Difficulties Verify that the baud rates and data framing parameters on all devices are the same With the pump chain a Communication timeout error on a computer is usually caused by the computer errantly handshaking on the RTS CTS and DSR lines Verify pins 4 5 and 6 on the 25 pin connector are jumped on the computer side of the cable PHD 4400 Display Messages Only if you have a optional display amp keypad Pump Stalled This indicates that the pusher block travel ha
11. 4 Programmable output aie al 5 Logic High Reference SV 6 mA Figure 12 User I O Connector Specifications 6 Foot switch input 7 Timer Input 9 Event input Rising edge starts pump Falling edge triggers program Falling edge stops pump event 8 Directional control input Logic Low OV 0 8V Rising edge sets pump to infuse Max 6 mA current sink Falling edge sets pump to refill Logic High 2V 5V Max 6mA current source Appendix J Accessories amp Spare Parts Auto Fill Accessory To operate the Auto Fill feature either the low pressure or high pressure valve assem bly is required Each assembly contains the solenoid valve connector cable power cable and tubing and fittings to connect to the syringe Operates at 115 230 VAC 50 60 Hz via a selector switch Cat No Accessories Cat No Spare Parts 72 2748 Daisy Chain Cable 1 8 m 6 tt 0606 086 Magnalube Lead Screw Lubricant 55 7760 Daisy Chain Cable 0 6 m 2 ft 4430 034 Syringe Clamp 70 2022 Daisy Chain Connector 9 PIN 5100 060 Thumb Screw Syringe Clamp 55 4145 Daisy Chain Connector 25 PIN 5100 059 T Knob Withdraw amp Anti Siphon 55 4144 Foot Switch Bracket 55 7002 Auto Fill Valve Box Regular 5406 002 High Force Modular Pumping Pressure to 20 psi Component Operators Manual 55 1145 Auto Fil Valve Box High Pressure 4430 066 6ft Motor Encoder Extension Cable 200 psi 4430 063 6ft DC Power Extension Cable 5108 032 Adjustable Stop 2400
12. 41 Figure 13 Auto Fill Valves rarnrnnrnrrrrrvnrvnnnvnnnonnrnnrnrrnnnnnnnen 43 Publication 5415 001 REV C s Harvard Apparatus High Force Modular Pumping Component User s Manual General Information Serial Number All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the underside of the mounting plate Calibration All syringe puimps are designed and manufactured to meet their performance spec ifications at all rated voltages and frequencies Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCI DENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclu sion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly cont
13. 95 or Windows 98 3 5 floppy disk drive Mouse VGA or higher display and driver capable of 800x 600 resolution one unused serial communication port Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix M Symphony Manual Contd 2 0 Connecting a serial cable from your pump to your PC Symphony is designed to work with genuine Harvard Apparatus pumps The model 44 PHD 2000 and PHD4400 pumps will work with Symphony Symphony will not work with the model Pump 22 or other older Harvard pumps Connect pump to your PC serial communications port with a four wire RJ11 data cable with straight through pinning Do not use a four wire telephone cable It uses crossed pinning and will not work Depending on your computer you may have either a DB9 DB25 or other connector on your PC serial port Refer to your PC hard ware manual if you re unsure of the serial communications port type and signal con nections Prepare an adapter to the RJ11 cable with the following connections RJ11 pin signal Example PC DB 9 pin 2 REC 3 3 XMIT 2 4 GND 5 Additional pumps may be connected directly to the first pump in a Daisy chain fash ion to the first pump Refer to your pump manual to set pump addresses protocol and baud rate Note the Harvard Model PHD2000 and PHD4400 pumps must be set in the Pump 44 communications protocol Refer to your pump user manual to veri
14. ASCII 42 decimal Note With a pump chain if no address precedes the command transmitted the pump at address 0 will respond to the command Commands Response CR LF prompt RUN Infuse forward direction REV Start reverse direction Not accessible on Infusion model STP Stop CLV Clear volume accumulator to zero CLT Clear target volume to zero MLM number Set rate units are milliliters per minute ULM number Set rate units are microliters per minute MLH number Set rate units are milliliters per hour ULH number Set rate units are microliters per hour MMD number Set diameter units are mm Rate is set to 0 MIT number Set target infusion volume units are ml Numbers between 0 and 1999 will be accepted by the pump Leading zeros and trail ing decimal point are optional Any number of digits to the right of the decimal point may be transmitted The number received will be rounded to four significant digits if the leading digit is 1 or three significant digits if the leading digit is 2 to 9 Publication 5415 001 REV C i Harvard Apparatus High Force Modular Pumping Component User s Manual Pump Chain Commands Contd Queries Response CR LF Value CR LF Prompt Queries with Numeric Response DIA RAT VOL TAR VER Send diameter value units in mm Send rate value in current range units Send current accumulated infused volume units are ml Send target volume units are ml Send model and version number Value f
15. ML Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Pump Chain Commands Contd MOD PMP VOL PGM Request to set or query pumping mode Set MOD PMP Puts pump in Pump Mode MOD VOL Puts pump in Volume Mode MOD PGM Puts pump in Program Mode Command will not be accepted if the pump is running and a Not Applicable response will be given Query MOD If mode is PUMP response will be PUMP If mode is VOLUME response will be VOLUME If mode is PROGRAM response will be PRGRAM DIR INF REF REV Request to set or query pumping direction Set DIR INF sets pumping direction to infusion DIR REF sets pumping direction to refill DIR REV reverses current pumping direction Command will not be accepted if the pump is running in volume or program modes and a Not Applicable response will be given Query DIR If pump direction is infusion response will be INFUSE If pump direction is refill response will be REFILL AF ON OFF Request to set or query auto fill setting Set AF ON turns Auto Fill feature on Note The syringe volume is also needed for auto fill to operate See SYR command AF OFF turns Auto Fill function off Command will not be accepted if the pump is running and a Not Applicable response will be given Query AF If Auto Fill function is ON response will be ON If Auto Fill function if OFE response will
16. Pumping Component would return to delivering its background rate of 300 ml hr waiting for another external event PROGRAM PRINTOUT PROG1 SEQ 1 TTL OUT 5 0000 ml OFF INFUSE PROG1 SEQ 2 EVENT PROG1 SEQ 5 TTL OUT GO TO 4 ON PROG1 SEQ 3 PUMP PROG1 SEQ 6 PROFILE 300 00 ml hr 75 000 ml mn INFUSE 10 0000 ml PROG1 SEQ 4 PROFILE INFUSE 75 000 ml mn PROG1 SEQ 7 RESTART Publication 5415 001 REV C i Harvard Apparatus High Force Modular Pumping Component User s Manual Programming Tutorial Contd LJ mti Figure 9 Use of TTL Signal Before the pumping flow begins the pump sends a TTL signal to another piece of equipment for 5 seconds but first verifies that the TTL line is low by turning it off for 1 second then turns it on The pumping process consists of an alternation of pump ing 3 ml s at 53 ml mn and 5 ml s at 75 ml mn It uses the TTL output to signal to another device whose sequence it is executing by lowering the TTL line before sequence 5 and raising it before sequence 8 PROGRAM PRINTOUT PROG1 SEQ 1 TTL OUT 53 000 ml mn OFF 3 0000 ml Prog1 SEQ 2 PAUSE INFUSE 0 00 01 INTERVAL PROG1 SEQ 7 TTL OUT PROG1SEQ 3 TTL OUT ON ON PROG1 SEQ 8 PROFILE PROG1 SEQ 4 PAUSE 75 000 ml mn 0 00 01 INTERVAL 5 0000 ml PROG1 SEQ 5 TTL OUT INFUSE OFF PROG1SEQ 9 GOTO 5 PROG1SEQ 6 PROFILE This is an example of the High Force Modular Pumping Component being operated from a remote location
17. Steel Syringe marnnrnnnnrrnnnnnvnnnnvrnnnnnrnnrnnrenenn 43 C Nominal Min Max Flow Rates 44 D Custom Applications osrrnnnnvrnnrnrrnnnnnrnnenrrnnnnrnnnnnnvnnenn 44 E Pressure and Force Specifications rrrrrrrrnnnnnrnnrnnr 45 F PHD 4400 to PC Connection seses 45 G RS 232 Specifications eeens 46 H Symphony Overview mrrrrnnnnvvnnrnrrrnnnnvnnenrrerrnrnnnrnnreernn 46 I User I O Connector Specifications rrrrrrrrrnennvnnrenr 47 J Accessories and Spare Parts arsrnnnrnvvnnnnrrnnnnvrnnrnnne 48 K Maintenance and Troubleshooting rrrnnnnrvrnnnnnnnnnnn 48 L Auto Fill Valves oeri e a 49 M Symphony Instruction Manual ssoeoorrreonnnrnnenrrrn 50 58 Figure 1 Syringe Loading errvnnnnvnnnonvvnnnnvrnnnnvennrnrrrnnnnnnnennr 10 Figure 2 External Connections rrsrrrrrrrronnrrrrrrrnnnrrrrrrnnnnnnr 17 Figure 3 Multiple Infusions srrrnnnonnnrnnnnvonnrnrnnnnrnnrnnnrnen annen 19 Figure 4 Ramping Up Infusion Rate 20 Figure 5 Multiple Dispensing cecene 21 Figure 6 Periodic Dispense LOOP 22 Figure 7 Comb Infusion amp Withdraw Profiles 006 23 Figure 8 Use of Events ccccececeeeeeeeneeeeeeeeeeeeeeeenennaeeaeees 24 Figure 9 Use of TTL Signal rrrnnnnrnnnonvvnrnnrnnnonnvnnnrrrnnnnnnnnennr 25 Figure 10PHD 4400 to PC Connection cccccsseeeeeseees 39 Figure 11 RS 232 Specifications erreen 40 Figure 12User I O Connector Specifications cce
18. be OFF Publication 5415 001 REV C J Harvard Apparatus High Force Modular Pumping Component User s Manual Pump Chain Commands Contd SYR lt float gt Request to set or query syringe volume setting for auto fill Used in conjunction with Auto Fill feature See AF command Set syringe volume SYR volume Volume is of format f f f f f f Units are ML If volume is accepted and valid volume will become the new syringe Auto Fill volume Volume will not be accepted if the pump is running and a Not Applicable response will be given If the volume is invalid an Out Of Range response will be given Query syringe volume SYR Response is of the following format space space ffffff Request to read the TTL logic level of the specified pin on the external 9 pin D SUB connector Valid pin numbers for input are 6 7 8 and 9 If the pin specified is valid and if the pin level is high response will be ON If the pin level is low response will be OFF If the pin specified is invalid An Out Of Range response will be given OUT d lt ON OFF gt Request to set the TTL logic level at the specified pin on the external 9 pin D SUB connector Valid pin number for output is 4 If the pin specified is invalid An Out Of Range response will be given Example Set pin 4 high OUT 4 ON Set pin 4 low OUT 4 OFF Publication 5415 001 REV C 4 Harvard Apparatus High Force Modular Pumping Component U
19. connected enabling the pump to infuse by weight instead of by volume Section 8 A printer can be connected to record final volumes or weights whenever the pump stops In addition the program entered for the program mode can be list ed on a connected printer Both a scale and a printer may be connected simul taneously See Section 8 Nonvolatile Memory All operational data entered into the pump from a computer will be stored including the program On power up the display will blink until the pump receives its first com mand and all settings from when it was powered down will be recalled Stall Detection An optical detector is used to verify expected movement of the motor If the motor is prevented from turning due to jamming or excessive back pressure the pump will stop An indication of PUMP STALLED will be displayed on the PC and on the optional display Gif attached Program Storage Programmable model can store up to 4 sets of 9 program sequences for later selec tion Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Installation Sturdy level clean and dry surface Minimum of one inch 2 5 cm clearance around the pump Appropriate environmental conditions Well ventilated area Mount into an appropriately rated cabinet or enclosure 5 000 m 12 70 Dimensions inches cm 28 89 11 375 11 00 28 0
20. min 140 mL max 0 001 uL hr with 0 5uL syringe 220 82 mL min with 140mL syringe 200 Ibs See Appendix E 1 8 Stepper Constant Current Chopper Drive 2A per phase max Microstepping from 1 2 to 1 32 2 1 1 2 optional 24 threads per inch 0 393 24 27 3 sec step 416 7usec step 0 18 um min 190 676 mm min Optional 2X20 Line VFD p n 2400 235 Optional p n 2400 252 RS 232 4 pin Header Friction Lock Molex or AMP 4 pin RJ 11 Telephone Jack 9 pin D Sub Female 12 to 40VDC 5 75W use only a SELV approved power supply 11 875 X 5 50 X 6 625 L X W X H 30 2cm X 14 0cm X 16 8cm 11 375 X 5 00 Mounting holes for 4 8 screws 28 9cm X 12 7cm 4 50 x 7 00 Mounting holes for 4 6 screws 8 5 Ibs 3 86kg 0 to 45 C 20 to 70 C 20 to 80 RH non condensing s Harvard Apparatus High Force Modular Pumping Component User s Manual Theory of Operation The High Force Modular Pumping Component employs a microcontroller which con trols a small step angle stepping motor that drives a lead screw and Pusher Block Microstepping techniques are employed to further reduce the step angle eliminating flow pulsation Data can be entered via an RS 232 connector located on the rear panel The microcontroller calculates the cross sectional area of the syringe selected and calibrates the flow rate and volume accumulation The numerous features of the High Force Modular Pumping Compone
21. the pump Pressing the foot switch performs the same func tion as pressing the RUN STOP key on the keyboard The foot switch connector allows remote or automated operation of the pump Timer Opening the timer input starts the pump Closing the timer input stops the pump The timer input allows for an externally controlled pumping interval Pumping Direction Sets the direction of pumping Opening the directional input sets the pump to infuse Closing the directional input sets the pump to refill The pumping direction input is recognized only in the situations that the INFUSE REFILL key would be recognized i e when the pump is stopped or running in the Pump Mode Valve Control The valve control output is an indicator of the direction of pump travel When the output is high the pump is set to Refill A low output indicates Infuse In the following examples the diameter is 26 7 mm and the infuse rate is 50 ml mn To run a program after entering it select Program mode using the SELECT MODE key and press the RUN STOP key The examples on the following pages include Multiple Infusions Ramping Up Infusion Rate Multiple Dispensing Periodic Dispense Loop Combination Infuse and Withdraw Profile Use of Events and Use of Signal OEM Configuration Factory Set 44 Protocol No Alarms Standby Mode On power up Publication 5415 001 REV C 3 Harvard Apparatus High Force Modular Pumping Component User s Manual Programming Tutorial
22. they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required s Harvard Apparatus High Force Modular Pumping Component User s Manual Table of Contents SUBJECT PAGE NO General Information Manual Description eerannrrrnnnnvnnvrnnnnnnnnrrnnnnnnnrrnnnrsnnrrrnnsennn 3 VWarrantyruqaermaaaaafreumsttiaturet Limtre 3 Repas Lunn gts Gear ride med 3 Serial Numbers rrrnnrrnnannvnnnnnrrnnnnvnnnenrrennnrnnannnenrnnrnesnnnnnsennr 3 Gibron S nesia e sendt a a a 3 General Safety Summary s nsssssnunnennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 4 5 Technical Specifications ss nvnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnne 6 Theory of Operation rrsnsannnnnnvnnnnnnnnnnnnnnnnnnnnnnnnnnnennnnnnnnnnnnnnnnn 7 Packing list issi ccs cn oes cci eee ect seeren tenke sae den decevestnapuanascceuederstes 7 Features Pressure and Speed rrnannnvonvnrrnnnnrnnnnnvonnnnrnnnnnnonnnnrnnnnnnnnnnn 8 Infusion and Refill Rates rrrnnrrnnnnonnrrnnnrrnnnrrnnrrrnnrrnnnrrnnnnn 8 Target Volume nona maraa aa aa ain 8 Auto Filiina aa ae aaaea eaaa aaea Mda ia 8 Modes of Operation cccccceececeeeseceeeeeeeeeeeeeeeeeeeessaaeeneees 8 External Connections ccccccceeeeeeceeeeeeseaeeeeeeeeeseaeeeseneees 9 Nonvolatile Memory rnnrannvrrnnnnnnnvrnnnnnnnnrrnnnnnnnrrnnnrnnnrrrnnsennn 9 Stall D tections u uva v26mm freda ASN es 9 Program Storage ccecececesceceeeeeeeeeeeceeeeees
23. 20 ml mn for 10 seconds with a profile operation 4 Stop the pump Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Programming Tutorial Contd Multiple Dispensing Example PROGRAM PRINTOUT PROG1 SEQ 1 DISPENSE 35 000 ml mn 15 000 ml 3 REPEAT INFUSE PROG1 SEQ 2 DISPENSE 65 000 ml mn 25 000 ml 2 REPEAT INFUSE PROG1 SEQ 3 DISPENSE 45 000 ml mn 17 000 ml 2 REPEAT INFUSE Figure 5 Multiple Dispensing PROG1 SEQ 4 STOP E E E EITEMHAL TANGGENS Tug Here a series of dispenses are programmed Each dispense is started by a trigger such as pressing the RUN STOP key or pressing an attached foot switch Seven dispenses are programmed three of 15 ml at 35 ml mn two of 25 ml at 65 ml mn and two of 17 ml at 45 ml mn The pump s display will show TRIGGER when it is waiting for a run trigger and lt or gt will not be displayed This is a Strategy 1 dispense A time interval of 0 is specified when entering a Strategy 1 dispense Since the total volume to be dispensed is 129 ml and the syringe volume is 50 ml the Auto Fill feature would be very useful with this program Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Programming Tutorial Contd Periodic Dispense Loop Example Figure 6 Periodic Dispense Loop This is an example of a series of periodic dis penses of varying vo
24. AL HYPERTERMINAL to start the Hyperterminal application If Hyperterminal is not available install it from the Microsoft Windows Operating System Install disks or CD ROM 3 Set up the appropriate COMPORT for the following Cosma Ta sel x Peet gr ie ae 1 Leie Gal ii ne ST Pe h md ifer fi Configure ASCII Setup Baud Rate 9600 Echo typed characters locally Data Bits 8 Line delay 0 Parity None Character delay 0 Stop Bits 2 Wrap lines Flow Cntrl None Emulation Auto Detect Publication 5415 001 REV C 3 Harvard Apparatus High Force Modular Pumping Component User s Manual HYPER TERMINAL Terminal Emulation Software ierat Faai ibah r beds i Len diniy F tiimari Arer aj p Se Conn seine f Titta TE Kjernen bates bn 4 Dels tees de rearing dass SPP HE ACD kring Erit a a Po feed tre roret v laesa Aone aca GET an Gunn permer kenty I Apparel heed ba coreg brer n r Fo arie etre sete ko Pbl Gb Weng ken Bol eed eres e EJ You may want to save the setup information under a descriptive filename 4 At the PC launch hyperterminal with the above setup specifications if it is not already running Type ver at the PC keyboard and verify PHD 1 2 is displayed at the PC terminal Note The PHD4400 OEM Module is preset to the MODEL 44 PROTOCOL 9600 Baud and ADDRESS 00 Type run to start the pump type stp to stop the pump After starting the p
25. Diameter 0 73 mm 1 03 1 46 2 30 3 26 4 61 mm 7 28 10 30 14 57 Hamilton Microliter Series Gastight Size 0 5 pl Diameter 0 103 mm 0 1457 0 206 0 3257 0 460 0 729 1 031 1 46 2 3 3 26 4 61 mm 7 28 10 3 14 57 23 0 32 6 Becton Dickinson Glass All Types Size 0 5 pl j 2 5 5 10 20 30 50 100 Diameter 4 64 mm 4 64 8 66 11 86 14 34 19 13 22 70 28 60 34 90 Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix B Stainless Steel Syringes 8 ml Harvard High Pressure Stainless Steel Syringes Harvard High Pressure Stainless Steel Syringes Harvard now offers a completely new line of Stainless Steel Syringes intended for high pressure applications with good resistance to most aggressive liq uids Wetted parts are 316 stainless steel or Viton Syringes are available in 20 50 100 and 200 ml sizes with removable replaceable tips Genuine SWAGELOK syringe to tube fittings are available in 1 16 1 8 and 1 4 inch sizes A luer lock end fitting is also avail able All tips are interchangeable with all syringes 20 to 200 ml in the series Both syringe barrel end plungers are 316 stainless steel A Viton O ring seal between top and end of the barrel insures against leakage Syringes are guaranteed to be leak free for pressures up to 700 psi All syringes are supplied with inside diameter dimensions for use with Harvard microprocessor contro
26. High Force Modular Pumping Component User s Manual MAI 70 2202 HARVARD APPARATUS Publication 5415 001 REV C WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any changes as
27. PROG1 SEQ 1 PROFILE PROG1 SEQ 5 PROFILE 75 000 ml mn 95 000 ml mn 43 155 ml 0 00 10 INTERVAL REFILL INFUSE PROG1 SEQ 2 PROFILE PROG1 SEQ 6 PROFILE 50 000 ml mn 30 000 ml mn 0 00 04 INTERVAL 0 00 05 INTERVAL INFUSE INFUSE PROG1 SEQ 3 DECR PROG1 SEQ 7 PROFILE 4 0000 DECR 65 000 ml mn 0 00 01 INTERVAL 0 00 05 INTERVAL 12 REPEAT INFUSE INFUSE PROG1 SEQ 8 DECR PROG1 SEQ 4 INCR 5 0000 DECR 5 0000 DECR 0 00 01 INTERVAL 8 0000 INCR 11 REPEAT 0 00 01 INTERVAL INFUSE 8 REPEAT PROG1 SEQ 9 RESTART INFUSE Publication 5415 001 REV C 4 Harvard Apparatus High Force Modular Pumping Component User s Manual Programming Tutorial Contd Use of Events i f al nn it A i mme ed A 1 Figure 8 Use of Events This is an example of the High Force Modular Pumping Component working inter actively with other laboratory equipment The pump will continuously pump at 300 ml hr until an external event a high to low transition at pin 9 possibly produced by another High Force Modular Pumping Component causes the pump to deliver a 15 ml bolus at 75 ml mn After delivering 5 ml of the bolus output pin 4 is set to a logic high for the duration of the bolus after which it is dropped This output pin can be attached to the timer input of another pump such as a Harvard Pump 22 or 44 to cre ate a precise mixture during the bolus After the bolus is completed the Pump 22 would be stopped and the High Force Modular
28. When the pump is powered on the position of the pusher block is unknown and must be homed to a known position At the syringe full position a limit switch is placed such that it is tripped by the push er block when the syringe is full The limit switch is connected to pins 4 and 9 pro grammable output and the event input on the user I O connector When the pump receives a start signal it first refills the syringe and stops when the limit switch is sensed The pump then waits for a start trigger and performs a dispense then refills the syringe and waits again for the next start trigger PROGRAM PRINTOUT PROG1 SEQ 1 EVENT 75 000 ml mn GO TO 7 PROG1 SEQ 7 DISPENSE PROG1 SEQ 2 TTL OUT 10 000 ml mn ON 0 0001 ml PROG1 SEQ 3 PAUSE 1 REPEAT 0 00 01 INTERVAL REFILL PROG1 SEQ 4 TTL OUT PROG1 SEQ 8 PUMP OFF 75 000 ml mn PROG1 SEQ 5 PAUSE 10 000 ml 00 00 01 INTERVAL INFUSE PROG1 SEQ 6 PUMP PROG1 SEQ 9 RESTART Publication 5415 001 REV C 3 Harvard Apparatus High Force Modular Pumping Component User s Manual HYPER TERMINAL Terminal Emulation Software Remote Control Via The RS 232 Interface Using HYPERTER MINAL PHD4400 OEM Module 1 Connect the telephone cable between the High Force Modular Pumping Component RS 232 IN port J3 and a PC s serial port using the telephone jack to 9 pin D sub adapter 2 On the PC running a Microsoft Windows Operating System select START PROGRAMS ACCESSORIES HYPERTERMIN
29. act Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 Outside the U S call 508 893 8999 Goods will not be accepted for return unless an RMA returned materials authori zation number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for com pletion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If prac tical enclose a sample or drawing We offer a complete reconditioning service CAUTION This pump is not registered with the FDA and c AUTI N is not for clinical use on human or veterinary FOR RESEARCH USE OHLY patients It is intended for research use only i i Publication 5415 001 REV C 4 Harvard Apparatus High Force Modular Pumping Component User s Manual General Safety Summary Please read the following safety precautions to ensure proper use of your modular syringe pump To avoid potential hazards and product dama
30. aeeseeeeeessaeeeseneees 9 Installation visuatbosilisseestne Hed dies Saeed 10 Operation Syringe Loading arrrnannvnnnonvnnnnnrnnnnnvnnnnnrrnnnnnnnersrrenannnnnennn 11 INfUse Rate Lunar erste eaerdint eaaa 12 Target Volume oons iienaa E Sti dete 12 PUTO Eldar heat ot Arete cata 12 Program Mode Program Description arnrvrnnnnnnnvrnnnnnnnvrrnnnnnnnvrnnnnernnrrnnsennn 13 Sequence of Operation arnnnannvnnnnrrvnnnnrnnnonrnnnnnrnnnrnnnne 14 17 Program Run Time Error Messages ecne 17 External Control and Interfaces RS 232 DEVICES ieiti aiiin innuna ieia 18 Configuring Pump for RS 232 Devices rrrnnnnvvnnnnrnnnnnnnnnnr 18 User I O Devices iiir eap airain 19 Programming Tutorial Multiple Infusion Example rernnnnvvvnnnnnnnvrnnnnnnnrrnnnnnnnnnnnnnn 20 Ramping Up Infusion Rate Example 21 Multiple Dispensing Example seeen 22 Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Table of Contents Contd SUBJECT PAGE NO Combination of Infusion and Withdraw Profiles Example 24 Use of Events oaii aiaa aaia aaia 25 26 Remote Control via RS232 amp Hyper Terminal Emulation software 27 28 Pump Chain Commands Model 22 Protocol seerias a a 31 32 Model 44 Protocol rarnrrnnnnnnnnvrnnnnnnnvrnnnnnnnnrnnnnnnnnrnnnnnennnnnnn 33 Pump Commands amp Responses urrnrrnnrnvvnrnnrrnnrnrvnrenr 34 41 Appendices A Table of Syringe Diameters c e 42 B Stainless
31. am window to create review edit save recall and down load programs A pump program once created can be downloaded to any pump in the communications chain Once there the pump will run the program NOTE Symphony is not a real time controller All pump control is performed by the pump micro controller In other words a program must be first created or loaded in the pump memory in order to run Refer to your pump manual for a list and explanation of program instructions Programs can be saved in vpp file format Five sample programs example1 vpp example2 vpp etc are included with Symphony Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix M Symphony Manual contd 5 4 Log Sheet HERE Fr HH FG dt PE re Eder m ia i is Le amp p Cee aaa 2Y22Sh ze s pep BP SS EAN my For TT at dr EEEEESEEE 575308 cal eh al aa afi The Symphony log sheet tracks the activities of your pump s in a log sheet that can later be reviewed by a Windows text editor or word processor program The log update period is selectable by the user Files are stored in vpl file format 5 5 TO display Symphony I O display shows the status of the input and output lines on the I O port DB 9 Refer to the pump manual for proper use of the I O lines Publication 5415 001 REV C J Harvard Apparatus High Force Modular Pumpin
32. ation of the program at the specified sequence and the operation of the pump will be according to this sequence Data Specified Go To sequence number Go To Causes the program to immediately continue operation at the sequence specified Data Specified Go To sequence number Pause Pump stops for specified time then continues with next programmed sequence Current program rate set to 0 with no change in units Data Specified Time interval Pump Runs the pump continuously at the specified flow rate without any pumping target This mode can provide a background flow rate while waiting for an external event to trigger a new sequence specified by the EVENT operation Data Specified Flow rate Pumping direction Publication 5415 001 REV C 3 Harvard Apparatus High Force Modular Pumping Component User s Manual Program Mode Contd TTL Out Programmable TTL Pin TTL output Pin 4 of the user I O connector can be set to a HIGH or LOW level from within a program Data Specified TTL pin level Restart Immediately restart program from the first sequence Data Specified None Stop Stops pump and the program terminates Data Specified None Program Runtime Error Messages using optional keypad amp display If while running a program an operation is requested that cannot be performed the pump will stop and an error message will be displayed Error messages will be dis played with the following format Progra
33. ce Sequence is repeated the specified number of times Travel direction is as specified Data Specified Strategy 1 Delta flow rate Volume Time Interval 00 00 00 Volume increment Number of repetitions Pumping direction Strategy 2 Delta flow rate Time Time interval Number of repetitions Pumping direction Decr Same as INCR except rate is decremented Publication 5415 001 REV C 3 Harvard Apparatus High Force Modular Pumping Component User s Manual Program Mode Contd Dispense Repeatedly dispense specified volume Runs at specified flow rate until a volume is pumped or a time interval has elapsed then pump will stop If no time interval was specified Strategy 1 the display will show TRIGGER and the next dispense will begin after an external or keyboard run command Otherwise the sequence will pause for specified time interval Sequence is repeated the specified number of times Travel direction is as specified Data Specified Strategy 1 Flow rate Volume Time Interval 00 00 00 Target volume Number of repetitions Pumping direction Strategy 2 Flow rate Time Target volume Time interval Number of repetitions Pumping direction Event Program Events A program event is an external event defined as a high to low tran sition on TTL pin 9 Within a program a one time event trigger can be set which watches for and acts upon the external event The triggered event causes an immedi ate continu
34. er s Manual Appendix M Symphony Manual APPARATUS HARVARD Symphony P Symphony the Windows Pump Manager version 1 2 Symphony Installation To install Symphony follow one of these procedures Windows 95 and 98 Users Insert Symphony disk number 1 in your floppy drive choose Run from the Start menu type a setup and press the Enter key Windows 3 1 Users Insert Symphony disk number 1 in your floppy drive from the Windows Program Manager choose Run from the File menu type a setup and press the Enter key The Setup program will issue a number of prompts Unless you have a reason to over ride the defaults it is strongly recommended that you press the OK or the Yes buttons to accept the installation default settings You must close all active applications programs before the setup program will run Standard Setup is recommended for new Symphony users CONTENTS 1 0 System requirements 2 0 Connecting a serial cable from your pump to your PC How to run Symphony with multiple pumps 3 0 Configuring Symphony to communicate with your PC 4 0 Running Symphony for the first time 5 0 The Symphony screens 5 1 Interactive pump control screen 5 2 Pump graph 5 3 Pump program screen 5 4 Log sheet 5 5 1 O control 6 0 Troubleshooting 1 0 System requirements Minimum equipment requirements Intel compatible 486 33 computer with 8MB RAM Hard disk drive with 2MB available space Windows 3 1 Windows
35. ere units is one of the following ml mn ul mn ml hr ul hr PGR Request for the rate of pumping set during the running of a program Response is of the following format space space f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr Publication 5415 001 REV C i Harvard Apparatus High Force Modular Pumping Component User s Manual Pump Chain Commands Contd DIA lt float gt Request to set or query syringe diameter setting Set diameter DIA diameter Diameter is of format f f f f f f Units are MM INFUSE and RFFILL rates will be set to zero and AUTO FILL will be set to off If diameter is accepted and valid diameter becomes new diameter Diameter will not be accepted if the pump is running and a Not Applicable response will be given If the diameter is invalid an Out Of Range response will be given Query diameter DIA Response is of the following format space space f f f f f f Units are MM TGT lt float gt Request to set or query target volume setting Set target volume TGT volume Volume is of format f f f f f f Units are ML If volume is accepted and valid volume becomes new target volume Volume will not be accepted if the pump is running and a Not Applicable response will be given If the volume is invalid an Out Of Range response will be given Query volume TGT Response is of the following format space space f f f f f f Units are
36. g Component User s Manual Appendix M Symphony Manual contd 6 0 Troubleshooting 6 1 Pump doesn t communicate with PC Check the following items if you re unable to communicate with your pump Check the pump to PC cabling Check your comm port selection matches Symphony set up Verify the pump baud rate is the same as Symphony baud rate Verify the pump address is correct Verify that your pump is set for Harvard pump 44 communications protocol 6 2 Setup program won t run when installing Symphony Make sure all other applications are turned off Symphony setup will not run correctly if other applications are running while attempting installation 6 3 Symphony screens don t all fit on my monitor screen Symphony screens are not scaleable This means that you may move them and close them like other Windows screen but you cannot change their screen size To gain screen space try increasing the resolution of your screen from the Windows control panel 6 4 The pump infuse and refill rates go to zero when I change syringe sizes Each time you change the syringe size you must enter a new infuse rate NOTE Sometimes when moving around between Pump and Program modes the infuse and refill rates get set to zero When this occurs you must enter new rate via the Interactive Pump Control Screen A change to correct this is planned for the next release 6 5 My pump program created in Symphony will not run correctly
37. ge use this product only as instructed in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired To Prevent Hazard or Injury Use Appropriate Power Supply Use only an SELV approved power supply Be sure to observe proper polarity of con nections when hooking up DC supply voltage CAUTION FAILURE TO MAINTAIN POLARITY MAY RESULT IN DAMAGE TO THE UNIT AND WILL VOID THE WARRANTY 24 Vdc positive PINS 1 amp 2 of connector P1 24 Vdc return PINS 3 amp 4 of connector P1 see input connections diagram Ground the Product This product is grounded through a ground stud located on the under side of the base plate To avoid electric shock the grounding conductor must be connected to earth ground Before making any connections to the input or output terminals of the prod uct ensure that the product is properly grounded Make Proper Connections Make sure all connections are made properly and securely Any signal wire connections to the unit must be no longer than 3 meters Avoid Contact with Heatsinks Some components normally operate at temperatures exceeding 70 deg C To avoid injury do not touch any heatsinks Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage i
38. ion to infuse Enter sequence s pumping direction Explanation PROGRAM ENTER Press until STOP selected Enters selection and ends program entry PROGRAM PRINTOUT PROG1 SEQ 1 PROFILE PROG1 SEQ 2 PROFILE Publication 5415 001 REV C 25 000 ml mn 5 0000 ml INFUSE PROG1 STOP SEQ 3 Harvard Apparatus High Force Modular Pumping Component User s Manual Programming Tutorial Contd Ramping Up Infusion Rate Example PROGRAM PRINTOUT PROG1 SEQ 1 PRO FILE 10 000 ml mn 0 00 01 INTERVAL INFUSE PROG1 SEQ 2 INCR 0 1695 INCR 0 00 01 INTERVAL INFUSE 59 REPEAT PROG1 SEQ 3 PRO FILE 20 000 ml mn 0 00 10 INTERVAL Figure 4 Ramping Up Infusion Rate INFUSE PROG1 SEQ 4 STOP In this example the pump will ramp up from 10 ml mm to 20 ml mn over 60 sec onds then continue to run at 20 ml min for another 10 seconds This is a Strategy 2 Program requiring four sequences 1 Specify the initial rate as a profile of 10 ml mn for one second 2 Specify the ramp up to 20 ml mn Since the minimum resolution of an increment is one second it will take 59 steps to reach the target rate Sequence 2 starts at time 1 second and ends at time 60 seconds giving it a duration of 59 seconds At one second a step 59 seconds divided by one second per step equals 59 steps The increase per step will by 20 ml mn minus 10 ml mn divided by 59 steps or 0 1695 rounded to four decimal places 3 Continue running at
39. ll types of custom pumping or pilot plant applications the pump s programming capabilities can be enhanced Please contact the Harvard Development Group if we can be of help Publication 5415 001 REV C J Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix E Pressure amp Force Specs PHO MPM LEAR PORCE sa MUSE AATE Byringe Oia Ji danas 2 200 sees ae ate l 3 A A F 3 rv 5 an y 150 h D er W a i apa a Fa ee Even k Deere av val 50 ef mem on ee 4 a LE a 0 S 100 150 Appendix F PHD 4400 to PC Connection Teepe a DB9 Female to tape at all Tiru 4400 ie reg 4 6 PIN RJ11 12 such as Modular Pump hisu Cece L COM P N RBAO98F ea Figure 10 PHD 44 to PC Connection Publication 5415 001 REV C I Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix G RS 232 Specifications Pump Chain Pump Port 1 Computer control side Pump Port 2 Connection for remainder of pump chain Baud Rate 1200 2400 9600 or 19 200 Word Size 8 Parity none Stop Bits 2 Factory set at 9600 baud Scale Connect scale to Pump Port 1 Supported scales Mettler Baud Rate 2400 Word Size 7 Parity even Stop Bits 1 Pause 0 Sartorius Baud Rate 2400 Word Size 7 Parity even Stop Bits 1 Code Settings 311 Weigh only models with PLUS performance package 211 External print command wi
40. lled pumps and rate charts for use with older classic pumps Replacement Viton O Rings are available as are the more chemically resistant Chemraz O Rings Harvard High Pressure 8 ml Stainless Steel Syringe This syringe has been designed to utilize the high forces available in our syringe pumps to produce pressures up to 1500 psi The syringe is constructed entirely of 316 stainless steel with two Chemraz O Ring seals two Teflon O Ring seals and 1 6 mm 1 16 in SWAGELOK end When this syringe is used in the 70 2200 or 70 2201 Harvard PHD 4400 pressures in excess of 1500 psi are consistently achieved for chromatography and process control applications Volume 8 ml Dimensions Length of Barrel 16 5 cm 6 1 2 in Plunger Excursion 11 4 cm 4 1 2 in oD 1 6 cm 5 8 in ID 0 95 cm 3 8 in Max Test Pressure 4000 p s i Working Pressure 1500 p s i Cat No Product 55 3369 Harvard High Pressure 8 ml Stainless Steel Syringe Replacement Parts 5013 089 Chemraz O Ring 20 ml 5013 090 Chemraz O Ring 50 ml 5013 091 Chemraz O Ring 100 ml 5013 092 Chemraz O Ring 200 ml 5013 087 Chemraz Tip Seal O Ring all sizes 72 2472 Replacement Viton O Ring 20 ml 10 72 2473 Replacement Viton O Ring 50 ml 10 72 2474 Replacement Viton O Ring 100 ml 10 72 2475 Replacement Viton O Ring 200 ml 10 72 2476 Replacement Viton Tip Seal O Ring 10 Harvard High Pressure Stainless Steel Syringes With SWAGELOK With L
41. lumes and intervals For this application Strategy 2 dispenses are used Note that between the third and fourth dis penses is a 45 minute interval Each dispense in the first sequence is separated by a pause interval of 1 30 Since after the third dispense there already will be a 1 30 pause an addi tional pause of 43 30 is used to extend the pause to the desired 45 00 Sequence 5 is a RESTART command causing the series of dis penses to be continuously repeated until the pump is stopped Publication 5415 001 REV C PROGRAM PRINTOUT PROG1 SEQ 1 DISPENSE 15 000 ml mn 3 5000 ml 0 01 30 INTERVAL 3 REPEAT INFUSE PROG1 SEQ 2 PAUSE 0 43 30 INTERVAL PROG1 SEQ 3 DISPENSE 25 700 ml mn 6 7500 ml 0 05 00 INTERVAL 2 REPEAT INFUSE PROG1 PENSE 20 000 ml mn 4 3000 ml 0 02 30 INTERVAL 4 REPEAT INFUSE PROG1 SEQ 5 RESTART SEQ 4 DIS Harvard Apparatus High Force Modular Pumping Component User s Manual Programming Tutorial Contd Combination of Infusion and Withdraw Profiles Example Here is an example of a more com plex profile program Each run of the infusion has been determined to pump 43 155 ml The first sequence refills the syringe with the volume to be infused then the infusion profile is started after which the syringe is refilled and the infusion is repeated until the pump is stopped i TIME esros Figure 7 Combination Infusion and Withdraw Profiles PROGRAM PRINTOUT
42. m N SEQ n message Where N is the Program number and n is the sequence number when an error was detected and message is the indicated error as follows INFINITE LOOP A GOTO sequence cannot specify the current sequence INVALID GO TO The target of the GO TO specified an invalid sequence number RATE UNDERFLOW A decrement sequence decremented a rate to less than or equal to 0 RATE OVERFLOW An increment sequence caused an arithmetic overflow OUT OF RANGE Specified or calculated rate is beyond the pumps capabilities with the specified syringe VOL TGT ERROR A sequence with a volume target cannot follow a sequence with a time target unless the volume delivered is zero or the pump is stopped at the start of the sequence entering it select Program Mode using the SELECT MODE key and press the RUN STOP key Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual External Control Interfaces RS 232 Devices ove a YEN ae HT re a a ma par Fr Va an r nn zg Gs mal es Da ees m bna LE RS aT BAE i E Pe Figure 2 External Connections External devices that can be attached to the High Force Modular Pumping Component are categorized into either RS 232 devices or user I O devices Pump Chains Scales and Printers are RS 232 devices all other devices are user I O devices See the Appendix G for specification details on attaching RS 232 device
43. nd press down firmly on the syringe barrel Secure in place by tightening the T knob screw 4 8 For syringes greater than 50 cc it may be necessary to use the V shaped clamp provided 6 Place clamp over the syringe barrel and secure to the holder block with the two long thumbscrews 5 Note If desired adjustable stops to limit the syringe plunger travel may be purchased from Harvard Apparatus see Appendix J for part number Install the stops on the two guide rods at the desired locations Infuse Rate The Infuse Rate is the rate of pumping while infusing in the Pump or Volume modes Also the Infuse Rate is used as a starting rate for the program mode if one is not spec ified in the program regardless of pumping direction Target Volume The Target Volume is the volume that you desire to deliver at the set Infuse or Refill Rate The pump must be set for this Volume mode for this feature to work When entering the Target Volume enter the volume desired in mls milliliters Auto Fill When set to ON the syringe is assumed to be empty Auto Fill continuously monitors the volume of the syringe according to the volume pumped When the pump deter mines that the syringe is empty the operation in progress is suspended and Auto Fill is activated The pumping direction is then reversed and the pump runs at the refill rate During the Auto Fill operation the display will indicate the volume of liquid in the syringe
44. nd the actual data for the operation such as rates and volumes The necessary data specified for each sequence will depend on the strategy used One of two strategies may be chosen for a sequence s target Strategy 1 Target Volume pumps until a target volume is reached while Strategy 2 Time Interval pumps until a target time interval has lapsed When Strategy 1 is used enter a time interval of 0 00 00 then you will be prompted for the target volume See the Programming Tutorial for example programs The following is a list of possible data that can be requested when entering a program and instructions on entering the data Publication 5415 001 REV C 3 Harvard Apparatus High Force Modular Pumping Component User s Manual Program Mode Contd Sequence of Operation Operations that can be selected are Profile Increment Inc Decrement Decr Dispense Event Goto Pause Pump TTL Out Restart Stop Rate If the Rate entered is invalid an error message will not be given at the immediate time of entry An Out of Range OOR error message will be given during the running of the program Delta Rate Enter the rate of Increment or Decrement desired The units of the rate cannot be specified Units will be the same as the units of the current pumping rate at the time the sequence is executed Target Volume Enter the required delivered target volume of the sequence For increment and decre ment sequences the target
45. nt result from the use of microprocessor technology The High Force Modular Programmable Pumping Component model provides full programmability along with Infuse Withdraw capability Retaining Brackets and Adjustable Stops are all described in detail on Page 11 of this manual This unit is designed to operate inside an appropriately rated fire electrical mechanical enclo sure or cabinet The board may be removed for remote operation Mounting holes configuration can be found on Pg 10 installation of this manual Packing List 1 Main Unit 2 6 ft motor encoder extension cable 3 6 ft DC power extension cable 4 RS232 cable 5 Symphony program disks 6 6 02z jar of lubricant Publication 5415 001 REV C 3 Harvard Apparatus High Force Modular Pumping Component User s Manual Features Pressure and Speed The High Force Modular Pumping Component can deliver up to 220 82 ml minute with a single 140 ml syringe Maximum pressure is dependent on syringe size If a syringe is to be used which is not listed in the syringe table Pg 36 enter the inside diameter of the syringe in millimeters Infusion and Refill Rates Specify independent rates for infusing and refilling This allows a slow infusion rate then a fast refill If Refill Rate is not set the software defaults to the set Infuse Rate value Target Volume Specify the volume that is to be infused or refilled The pump will run at the rate spec ified until this vol
46. ormat nnnn nnn The transmitted value is an 8 character string with leading zeros converted to SP char acters ASCII 32 The fifth character is a decimal point ASCII 46 Query Response CR LF Range CR LF Prompt Queries with String Response RNG Send range message Range is a character string one of ML H ML M UL H or UL M Error Responses CR LF CR LF prompt Unrecognized command CR LF O OR CR LF prompt Out of range Publication 5415 001 REV C 4 Harvard Apparatus High Force Modular Pumping Component User s Manual Pump Chain Commands Contd Pump Chain Commands Model 44 Protocol Symbol lt a 3 e o DRN R lt cr gt lt lf gt lt float gt lt integer gt lt time gt lt text gt Meaning optional select one either or digits 0 9 or a decimal point digits 0 9 carriage return ASCII 13 line feed ASCII 10 ffffff ddddd d dd dd any string of ASCII characters Command Formats and Meanings Command Format lt cr gt pump address lt cr gt optional pump address command lt cr gt Meaning Stops all pumps All pumps on the pump chain interpret this as a stop command Request for prompt The pump with the indicated address responds with its prompt Send a command to a pump The pump with the indicated address executes the command then responds with its prompt The optional pump address if not specified will default to pump address 0
47. p when Symphony started Mode 3 Use this mode if you re controlling a small number of pumps and want them to do different tasks Your PC must have a separate comm port for each pump Connect each separate comm port directly to each syringe pump Launch a separate instance of Symphony for each pump NOTE pumps must not be chained together You need a comm port for each pump to run in this mode Most PC s have only one or two available comm ports Running multiple pumps in this mode may fill up your PC screen To save space close down all unused screens 3 0 Configuring Symphony to communicate with your PC When starting Symphony all Symphony windows will open automatically To simplify your start up close all Symphony windows except the interactive pump control screen window From the interactive pump control window select view configuration pull down menu This pop up window allows you to select the comm port you wish to use and the baud rate Select either comm 1 2 3 or 4 and 1 200 2 400 9 600 or 19 200 baud Note Select a comm port that is not in use by other PC devices mouse modem etc The baud rate that you select here should match the baud rate set in the pump Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix M Symphony Manual contd sy mppery virina 12 Coppright S 1996 1997 1898 2001 Harvard Apparatus Check for proper communications this wa
48. repetition count Response lt float gt Query output pin level setting Response lt ON OFF gt Query pumping direction Possible responses SEQ n GOT SEQ n TGT SEQ n INT SEQ n RPT SEQ n OUT SEQ n DIR Set mode of program sequence n SEQ n MOD mode Where mode is as follows Mode Description STP stop PRO profile INC increment DEC decrement DIS dispense PAS pause INFUSE REFILL Mode Description RST restart EVN event GOT go to OUT set output pin PMP pump Set data item of program sequence n Set sequence s rate SEQ lt n gt RAT lt float gt lt units gt Rate is of format f f f f f f Units are one of UM UH MM MH Publication 5415 001 REV C Description ul mn ul hr ml mn ml hr 4 Harvard Apparatus High Force Modular Pumping Component User s Manual Pump Chain Commands Contd Set sequence s go to sequence number SEC lt n gt GOT lt n gt Set sequence s target volume SEQ lt n gt TGT lt float gt Set sequence s time or target SEQ lt n gt INT lt time gt Set sequence s repetition number SEQ lt n gt RPT lt integer gt Set sequence s pumping direction SEQ lt n gt DIR lt INF REF gt Set sequence s output pin level SEQ lt n gt OUT lt ON OFF gt VER Request for version of pumps embedded software Pump Chain Error Messages Error messages are in the format lt If gt space space lt message gt lt cr gt
49. s and Appendix I for user I O specifications There are two telephone jack type connectors on the back of the unit See Figure 2 These are the RS 232 ports Looking at the back of the pump the connector on the right is IN and the left is OUT Attach the RS 232 connectors in the appropriate port according to the following chart Device Port Pump Chain computer side IN Pump Chain pump side OUT Scale IN Printer OUT Configuring the Pump for RS 232 Devices Set at 44 Protocol The High Force Modular Pumping Component is factory set at pump chain address 00 baud rate 9600 Other configurations can be made upon request of customer Pump Chain Only with keypad Enter the 2 digit address assigned to the pump Note Each pump in the chain needs a unique address After entering the address up to 99 the baud rate will be request ed Use the RS 232 key to toggle between the supported baud rates 1200 2400 9600 and 19200 Note Each pump in the chain must have same baud rate See the Section 10 Pump Chain Commands for pump chain control information Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual External Control Interfaces User 1 0 Devices The pump does not need to be configured to attach a user I O device Simply plug the device into the 9 pin connector on the rear of the pump See Appendix I for wiring specifications Foot Switch or Relay Used to start and stop
50. s been impeded This may be caused by the syringe plunger hitting bottom a kink in the tubing syringe plunger binding or any situation requiring more force to the head of the syringe than the pump is capable of delivering To restart the pump remove the cause of stalling and press RUN The pump will continue the interrupted procedure where it stopped Also note a large increase in the pumping rate could also stall the motor Out of Range A value was entered or encountered in a pump program that was beyond the pump s limits Also verify that the Infuse Rate is not 0 Note that rates must be less than 42949 Express larger rates using different units EEPROM Data Lost The values stored in the nonvolatile memory have been lost This should never occur under normal operation Replace NOVRAM The nonvolatile memory has failed This should never occur under normal operation Publication 5415 001 REV C 5 Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix L Auto Fill Valves There are two Auto Fill Valve models available Catalog No Product 55 7002 Supplied with 1 8 inch I D 1 4 inch O D silastic tubing and connector to syringe 55 1145 Supplies with 1 4 inch O D 304 stainless steel tubing with Swagelok fittings Setting Up the Valve Set up consist of three phases Voltage selection 110 or 220 V Pump to valve elec trical connection and Valve tubing to syringe hook up it 1 Vol
51. s suspected on or to the product do not operate the product Contact qual ified service personnel to perform inspection Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual General Safety Summary Place Product in Proper Environment Enviromental Conditions Indoor use only Temperature 4 C to 40 C 40 F to 104 F Humidity 20 to 80 RH Well Ventilated Room Altitude up to 2000 m Mains Voltage Fluctuation not to Exceed 10 of Nominal Transient Overvoltage Category II Pump is Rated Pollution Degree 2 in Accordance with IEC 664 Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION Refer to Manual Protective Ground Terminal Caution Pinch Hot Hazard Surface Caution ESD Sensitive Device Publication 5415 001 REV C Specifications 3 Specifications Accuracy Reproducibility Syringe Sizes Flow Rate Min Max Linear Force Drive Motor Control Pulley Ratio Lead Screw Pitch Step Rate Min Max Pusher Travel Rate Min Max Display Keypad Interface Connectors DC Power RS 232 User I O Harvard Apparatus High Force Modular Pumping Component User s Manual Power Dimensions Mounting Dims Control Board Mounting Dims Weight Environmental Operating Temp Storage Temp Humidity Publication 5415 001 REV C 1 0 1 0 5uL
52. se is given lt float gt lt units gt Request to set or query infusion rate setting Set infusion rate RAT rate Set infusion rate and units RAT rate units Rate is of format f f f f f f Units are one of Definition UM ul mn UH ul hr MM ml mn MH ml hr If rate is accepted and valid rate will become the new infusion rate If the rate is invalid an Out Of Range response will be given Command will not be accepted if the pump is running in the Program Mode and a Not Applicable response will be given Query infusion rate RAT Response is of the following format space space f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr Publication 5415 001 REV C 4 Harvard Apparatus High Force Modular Pumping Component User s Manual Pump Chain Commands Contd RFR lt float gt lt units gt Request to set or query refill rate setting Set refill rate RFR rate Set refill rate and units RFR rate units Rate is of format f f f f f f Units are one of Definition UM ul mn UH ul hr MM ml mn MH ml hr If rate is accepted and valid rate will become the new refill rate If the rate is invalid an Out Of Range response will be given Command will not be accepted if the pump is running in the Program Mode and a Not Applicable response will be given Query refill rate RFR Response is of the following format space space f f f f f f units Wh
53. ser s Manual Pump Chain Commands Contd SEQ lt integer gt lt entry gt Request to set or query programming sequences lt integer gt is sequence number Default is Sequence 1 Command only applicable while pump is stopped Valid sequence numbers n are 1 to 10 n defaults to 1 wherever it is optional See program examples Query entire program SEQ Example response SEQ 1 DISPENSE 75 000 ml mn 43 155 ml 0 00 01 INTERVAL 3 REPEAT INFUSE SEQ 2 PROFILE 100 00 ml mn 150 00 ml REFILL SEQ 3 RESTART Query program sequence n SEQ n Example response to the command SEQ 2 with the previous example s program SEQ 2 PROFILE 100 00 ml mn 150 00 ml REFILL Query program sequence n s mode SEQ n MOD Response will be according to the following table Response Description Response Description STP stop RST restart PRO profile GOT go to INC increment EVN event DEC decrement PMP pump DIS dispense OUT TTL out PAS pause Publication 5415 001 REV C 3 Harvard Apparatus High Force Modular Pumping Component User s Manual Pump Chain Commands Contd Query data item of program sequence n Command SEQ n RAT Description Query rate Response f f f f f f units Where units is one of the following ml mn ul mn ml hr ul hr Query go to sequence number Response lt n gt Query target volume Response lt float gt Query time interval Response lt time gt Query
54. t pump in the PUMP CHAIN address 00 to the RS232 IN port of the next pump in the chain The address of the second pump should be set to 01 Commands at the PC terminal must include the pump address to control all pumps other than pump 0 e lrun lver 1stp Publication 5415 001 REV C 3 Harvard Apparatus High Force Modular Pumping Component User s Manual Pump Chain Commands Note Changing pump addresses is only possible with the optional display and keypad The Pump Chain RS 232 interface allows up to 100 pumps and in certain cases other RS 232 devices to be controlled from a single RS 232 communication port on a com puter Assign each pump in the pump chain a unique address from 0 to 99 This address is used to identify which pump is to receive a command and which pump is respond ing Configure each pump with its assigned address and the baud rate being used See Section 10 External Control amp Interfaces When the control device communicates with the pump a diamond appears on the far right of the display indicating that it is receiving RS 232 commands The diamond remains on the display until the pump is turned off or SET RS 232 is entered on the keyboard indicating a change in the RS 232 configuration A pump will not respond to pump chain communication while it is in a setting mode entered when user presses the SET key on the optional keypad The pump can still be controlled from the keypad while it is in a pump chain
55. tage Selection Valves are shipped from the factory with an American line cord with three wire molded power connector and the pump set for 115 125 VAC For 220 240 VAC operation the molded power connector must be cut off and replaced with an appropriate type The line cord is color coded in International standard colors Brown High Blue Neutral Green Ground Observe the polarities when installing the new connector To adjust the valve for 220 240 volt operation an international voltage selector switch must be set Remove the top panel by removing eight screws four on each end of the valve Identify the voltage selector switch located directly below Using a screw driver turn the switch from 110 to 220 The pump is now set up for 220 volt operation 2 Pump to Valve Electrical Connection The cable fitted with a male 9 pin D sub connector must be connected to the female D sub connector on the rear of the High Force Modular Pumping kit Power can now be switched on with the illuminated power On Off switch on the front valve housing 3 Pump to Valve Tubing Connection Swagelok fitting should be finger tight plus 1 1 4 turn Tubing is 304 stainless steel 1 4 inch O D wall thickness 0 035 inch o mi kifu b i cd ko s r a ine Silicon Tubing 1 4 OD 1 8 ID Acall Fa Figure 13 Pump to Valve Tubing Connection Publication 5415 001 REV C z Harvard Apparatus High Force Modular Pumping Component Us
56. th out stability 225 2400 baud 234 Even Parity 432 Beeper off Pork Pari pat fing Figure 11 RS 232 Connection Ohaus Baud Rate 2400 Word Size 8 Parity even Stop Bits 1 Auto print feature off Stable data only off Serial data frame selection Fr 7 Printer Serial printer with print buffer Baud Rate 2400 Word Size 8 Parity none Stop Bits 2 Appendix H Symphony ncuded with oem rio Symphony the Windows Pump Manager software allows you to control your High Force Modular Pumping Component from your remote computer With Symphony you can orchestrate all pump functions from a virtual control panel via the serial com munications link built into every High Force Modular Pumping Component See Appendix M for complete manual Catalog Number Product 70 3000 Publication 5415 001 REV C Symphony Windows Pump Manager Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix I User I O Connector Specs User I O input output connec ETE 1 tions are via a 9 pin D sub type connector All inputs are inter nally pulled up to a logic high Pin Assignments i iT al 1 Vss Ground reference e F TF logic low DOUD i Falling edge starts stops i 0 o l pump KLE 2 Valve control output LE t Low Infusing Har ars i High Refilling 3 Running Output Indicator a Low Pumped stopped gt High Pump running way
57. uer Lock Diameter Syringe Size 1 16 inch 1 8 inch 1 4 inch 20 ml 55 4199 55 4205 55 4209 55 4214 50 ml 55 4200 55 4206 55 4210 55 4215 100 ml 55 4201 55 4207 55 4211 55 4216 200 mi 55 4202 55 4208 55 4213 55 4217 Replacement Tips Furnished with Sealing O Ring 55 4220 55 4221 55 4222 55 4223 Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix C Min Max Flow Rates Minimum flow rates are taken from the smallest inside diameters and maximum flow rates are taken from the largest inside diameters of the syringes supplied by the eleven most widely used syringe manufacturers Nominal Minimum Maximum Flow Rates for Various Syringes Actual Limits will vary depending on manufacturer Syringe pl hr ml min Size Min in Min 0 5 pil 0 0001 95 330 1pl 0 0002 190 70 2 ul 0 0004 381 30 5 pl 0 0010 953 17 10 pl 0 0019 1 9013 25 pl 0 0046 4 7752 50 ul 0 0092 9 5511 100 pl 0 0183 19 153 250 pl 0 0454 47 532 500 pl 0 0911 95 492 1000 pl 0 0031 190 95 1ml 0 0033 205 30 2 ml 0 0119 747 35 2 5 ml 0 0076 476 21 3 ml 0 010 11 231 5 ml 0 0208 21 781 10 ml 0 0301 31 486 20 ml 0 0523 54 804 30 mi 0 0673 70 518 50 ml 0 1019 106 76 100 ml 0 1740 182 40 140 ml 0 2106 220 82 Appendix D Custom Applications The Harvard High Force Modular Pumping Components lend themselves to a multi tude of OEM industrial applications For a
58. ume has been delivered when in the Volume mode Auto Fill Auto Fill automatically activates an externally attached solenoid refer to Appendix L for part number and refills the syringe when it is empty This permits infusions to be virtually independent of syringe capacity Modes of Operation Set for pump mode can be changed thru RS232 Pump Runs continuously in the infuse or refill directions until stopped Volume Runs until a specified volume has been pumped or refilled Program Pump operates according to a specified sequence of instructions Note All modes interact with Auto Fill CE Mark Approved The High Force Modular Pump meets all relevant european EMC and safety require ments for laboratory equipment when used in accordance with the guidelines out lined in this manual Publication 5415 001 REV C 5 Harvard Apparatus High Force Modular Pumping Component User s Manual Features Contd External Connections User I O Allows pump operations to be synchronized with external devices or by a per son at a distance from the pump Connector pins are available to control direc tion of pump travel to control an external valve for refilling and for general use A simple contact closure to ground or TTL level signals may be used for inputs See Section 8 and Appendix I RS 232 Multiple pumps can be daisy chained together and remotely controlled from a computer or any device communicating via RS 232 A scale can be
59. ump 0 gt should be displayed indicating address 0 pump is infusing After stopping the pump 0 should be displayed Optional For users that have purchased and connected the optional display and keypad the following additional operations are possible Changing the RS 232 Communications Protocol At the PHD4400 keypad press and release the SET key and press and release the 1 key Verify MODEL 44 PROTOCOL is displayed To toggle to the MODEL 22 PROTOCOL press and release the 1 key again and then the ENTER key Otherwise just press the ENTER key to keep the MODEL 44 PROTOCOL Note Be sure to use the appro priate command set for the protocol selected Changing the Pump Address and Baud Rate Press and release the SET key and then the RS 232 key Verify PUMP CHAIN is displayed if not toggle through the modes by pressing the RS 232 key until it is Press and release the ENTER key Verify ENTERADDRESS 00 is displayed Use the numeric keypad to enter the desired address and press the ENTER key Verify BAUD RATE 9600 is displayed Toggle through the available Baud Rates by pressing the RS 232 key and then press and release the ENTER key to accept the desired baud rate Publication 5415 001 REV C 5 Harvard Apparatus High Force Modular Pumping Component User s Manual HYPER TERMINAL terminal Emulation Software Daisy chaining Pumps To daisy chain pumps connect another telephone cable between the RS232 OUT port of the firs
60. volume is an incremental target An incremental target is added to the delivered volume at the start of the sequence Time Interval Enter the time duration of the sequence in the form hours minutes seconds If sequence Strategy 1 Target Volume is used enter 0 00 00 for the time target The maximum time interval is 9 99 99 Number of Repetitions Enter the number of times the sequence is to be repeated The repetition number can be from 1 to 99 999 Pumping Direction Each sequence that specifies a pumping operation also specifies a pumping direc tion Pin Level Select either HI or LOW for the logic level of the programmable output pin 4 Go to sequence number Enter the destination sequence to continue operation of the program Valid sequence numbers are 1 to 10 Publication 5415 001 REV C 4 Harvard Apparatus High Force Modular Pumping Component User s Manual Program Mode Contd Profile Runs at specified flow rate until target volume is pumped or a time interval has elapsed Travel direction is as specified Data Specified Strategy 1 Flow rate Volume Time Interval 00 00 00 Target volume Pumping direction Strategy 2 Flow rate Time Time interval Pumping direction Incr Increments current rate by specified value and pumps until the target volume is pumped or a time interval has elapsed Units of rate will be that of the current rate of the pump or the infusion rate s units if first sequen
61. y Symphony will read the PHD 2000 and PHD 4400 firmware revision from the help view pop up screen If the revision field is blank that means your pump to PC communications link is not working For help solving communications problems refer to the communications trouble shooting sec tion On the interactive pump control screen there is an active status indicator to report communication status It blinks in the color green if communications are normal and it blinks in the color red if you have a communication error Symphony will not oper ate correctly if you have a communications error The most common sources of communication errors are caused by incorrect cabling and communication parame ters 4 0 Running Symphony for the first time Once you re communicating successfully with the pump green blinking Comm Active indicator familiarize yourself with Symphony by practicing with the interac tive pump control screen to change pump values You change pump parameters using common Windows controls such as direct numeric entry pull down menus on off buttons and spin controls Publication 5415 001 REV C Harvard Apparatus High Force Modular Pumping Component User s Manual Appendix M Symphony Manual contd 5 0 The Symphony screens 5 1 Interactive pump control screen a DERI Hyma HARVARD pem A FP PA AR AT USS alal riker C Aami kl l le Lal tenes Deberigd Jarra Drkrass a0 pa aie FG ETTE TE

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