Freedom Rocker™ BlotBot
Contents
1. Do not open the window when the BlotBot is in use Use recommended accessories only Substitutions may result in unsatisfactory performance Do not remove any panels or screws No user serviceable parts are behind the panels For indoor use only Pollution Degree 2 per EN 61010 1 Overvoltage Category II per EN 61010 1 Enclosure Protection Not Protected Against the Ingress of External Moisture Do not immerse in liquid NEXT gt gt 10 ADVANCE Operator s Manual for the Freedom Rocker BlotBot DISCLAIMER Next Advance Inc makes _ no representations or warranties expressed statutory or implied regarding the fitness or merchantability of this product for any particular purpose In no event shall Next Advance be liable for incidental or consequential damages Next Advance Inc is not liable for any damages including but not limited to lost profits lost savings or other incidental or consequential damages arising from ownership or use of this product or for any delay in the performance of its obligations under the warranty due to causes beyond its control Next Advance Inc also reserves the right to make any improvements or modifications to the product described in this manual at any time without notice of these changes Next Advance Inc products are not designed intended or authorized for use in applications or as system components intended to support or sustain human life as a clinical med2. After a few seconds the home screen will display Program or _ select your preferred Western blot protocol see Programming the BlotBot below Fill the buffer bottle Ensure that the hose hanging from the cap reaches the bottom of the bottle Leave the cap loose to allow air into the bottle as liquid is pumped out To fill the buffer hose select Flush from the home screen menu and press the Accept button The reagent slider will move forward until the buffer dispensing nozzles are over the back of the sample tray Press the Fill button until wash buffer is dispensed from both nozzles This may take several seconds When the tubing has been filled press the Drain button to remove the dispensed buffer from the sample tray Check each antibody reagent tube to make sure the foil seal is intact and firmly attached If you are using primary and secondary antibodies you will need two tubes for each blot you are processing Screw one tube into the primary antibody position toward the back of the slider and the other into the secondary antibody position just behind the buffer dispensing nozzle Pour or pipet the appropriate antibody solution or other reagent into each tube A standard sized mini blot processed in a mini blot tray requires 3 5 mL of antibody solution per tube but you may use more reagent if you prefer Place your blot s into the sample tray along with your blocking buffer or other N
3. dial and press the Edit button to edit the program Step 1 Choose a name for your protocol Select letters by turning the dial and press the dial to switch between letters and numbers symbols Use the cluster of four buttons at the lower left of the screen to navigate back and forth between letters and to delete letters or add spaces When finished press the Save button to save your program name and then press the Continue button to move to the next screen Step 2 On this screen select whether the protocol will be used with a mini blot tray 2 chambers with a central divider or a midi blot tray one large chamber If you typically run mini blots use the mini blot tray even if you plan to run only one blot at a time it will save reagents Press the Save button and then press the Continue button to move to the next screen Step 3 Now choose whether you want a clearing rinse to be included in the protocol A clearing rinse will briefly rinse the blot with 10 mL of wash buffer at the end of each step If you want a clearing rinse highlight Enable on the screen and then press the Save button Press the Continue button to continue NEXT DD 6 ADVANCE Operator s Manual for the Freedom Rocker BlotBot Step 4 On the last screen input the times and reagents for each step in your protocol Buffer wash steps can be repeated up to 10 times but reagent steps cannot be repeated Wh
4. has two windows work areas and doors on the left and right and the control panel and display in the center 1 Display 2 Control Buttons 3 Window Knob 4 Work Area visible through the window 5 Waste tank compartment A Caution Follow the Instructions in the Operator s Manual R Please dispose of the tubes trays and BlotBot in accordance with local S regulations NEXT gt gt 2 ADVANCE Operator s Manual for the Freedom Rocker BlotBot The BlotBot Work Area 1 Antibody reagent tubes 2 Wash buffer tubing 3 Rocker cradle 4 Tray cover 5 Sample tray 6 Reagent slider 7 Cover retainer clip 8 Recovery funnel 9 Waste funnel 10 Funnel tray Blots to be processed are placed in the sample tray The mini blot tray which has a central divider can be used for mini blots and strips Larger blots can be processed using the midi blot tray The reagent slider positions the antibody reagent tubes and the wash buffer nozzles over the sample tray when needed Waste liquids are dumped into the waste funnel which directs them into the waste tank Reagents that are to be recovered for reuse are directed into the recovery funnels The antibody tubes sample tray funnel tray and waste tank are easily removed from the machine for cleaning Other components are designed to remain in the instrument NEXT gt gt 3 ADVANCE Operator s Manual for the Freedom Rocker BlotBot Table of Icons Be B Can
5. EXT DD 5 ADVANCE Operator s Manual for the Freedom Rocker BlotBot starting reagent or buffer If you are using a mini blot tray which has a central divider make sure the blot is on the same side of the divider as the appropriate antibody tubes and use no more than 50 mL of blocking buffer per side If you are using a midi blot tray you may use up to 100 mL blocking buffer Replace the tray cover and fasten it with the retainer clips Close the waste tray compartment and the work area window Select the desired protocol from the menu and press the Run button On the next screen select which trays will be used for the protocol Follow the instructions on the screen to enable or disable individual compartments Disabled compartments will not have buffer dispensed into them When you are done press the Continue button to proceed If you have not already done so check the waste tank to make sure that it is empty and place 50 ml tubes into the tube holders if you plan to recover antibody Once this has been done close the waste tray compartment and press the Run button to start the run PROGRAMMING THE BLOTBOT The BlotBot is designed to work with your own laboratory protocols To program a new protocol follow these Steps From the home _ screen _ select Programs You will see a list of programs which have been populated with default values Select one of the programs using the
6. Operator s Manual The Freedom Rocker BlotBot Congratulations Contents Congratulations on your Parts of the BlotBot c eee 2 purchase of a Freedom Rocker The BlotBot Work Area 3 BlotBot by Next Advance Inc Table of 1CO0S sisseseiccecsseessaxeseceeseents 4 We re sure that once you use it you BUND sac setae ceacteees cane vdavaseaesuniacsteevene 5 will never want to process your Operai Oissar 5 Western blots manually again Programming the BlotBot 6 Please read this operator s After the run sosicsiecdessalseseiaaselecanevaies 7 manual which explains proper Maintenance ccccccsscssssessesseees 7 operation of the instrument This Troubleshooting cccccseceeseeees 8 manual is posted on our website SII 8 0 0 ee eee eee eee eer 8 www nextadvance com Click on the SPCCITI CAL ONS saies sssslsazanvecearanesesaaeies 9 SUPPORT button and then on the W Atrani eric sccanutedseeresucerpnseusmesasseeo 9 appropriate link to the manual Warnings and Cautions 10 We re confident that your Disclaimer ccccccccessceceeeeeeceeees 10 BlotBot will become an essential tool Contact Inf0 ccccccceceeeeeeeeeees 11 in your laboratory and we wish you success with your work rev 15 F12 NEX L gt ADVANCE Operator s Manual for the Freedom Rocker BlotBot Parts of the BlotBot Model 120 shown Model 240
7. accident product modification exposure to radiation other than for sterilization connection to an improper electrical supply lack of proper maintenance contamination improper installation or misuse If the product is used in a manner not specified by _ the manufacturer the protection provided by the equipment may be impaired The warranty shall also not apply to defects arising from fire flood lightning or other conditions unrelated to correct operation of the Product Next Advance s liability is limited at the company s election to 1 refund of the original purchaser s purchase price for the Product 2 repair of the Product or 3 replacement of the Product or defective parts Evidence of purchase by the original purchaser is required Next Advance may also request documentation of proper maintenance if applicable Next Advance makes no other warranty expressed or implied with respect to its Products NEXT ADVANCE MAKES NO WARRANTY RESPECTING THE MERCHANTABILITY OF THE PRODUCTS OR THEIR SUITABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE OR USE Next Advance shall not be liable for indirect special incidental or consequential damages of any nature Any recovery for any claim shall be limited to the original purchase price for the product OPERATOR S RESPONSIBILITY Provide proof of purchase and provide normal care and maintenance WARNINGS AND CAUTIONS Read the user s manual before operating
8. cel Stop Drain 4 B Figure 3 Button icons used on the BlotBot The functions of particular buttons are explained in the text v X NEXT gt gt 4 ADVANCE Operator s Manual for the Freedom Rocker BlotBot SETUP Remove all tape and packing materials from the BlotBot and place it on a stable level lab bench Open the door s and remove the funnel tray Unscrew the caps from the 50 mL recovery tubes and place them in the brackets to each side of the waste tank Replace the funnel tray and close the door Open the transparent window that protects the work area see Figure 2 Place the sample tray into the rocker cradle and fold the flaps over toward the center of the tray The spikes on the tray should be facing toward the back of the instrument Place the tray cover onto the sample tray and clamp it down by rotating both of the cover retainer clips See Figure 2 for reference The wash buffer connector and the power supply are located at the back of the instrument Attach the wash buffer hose to the connector using the quick connect fitting Plug the power supply into the power jack on the back of the BlotBot and insert the plug into a wall outlet The machine is now set up OPERATION Ensure that the window s and door s are completely closed Turn the power switch on the back of the unit to the on position The display will illuminate and the moving parts will return to their home positions
9. en selecting the reagent you can choose the following Buffer A Buffer B Reagent 1 Reagent 2 and END Selecting END indicates that there are no more steps For example a program with 6 steps whereby for step 1 the blocking solution is already in the tray may have the reagents set as follows REAGENT STEP 1 STEP 2 STEP 3 BUFFER A EAGENT 1 R STEP 4 BUFFER A R EAGENT 2 BUFFER B END STEP 5 STEP 6 STEP 7 You should also indicate whether you want your antibody solutions to be recovered for future use The BlotBot 120 can recover one reagent and the BlotBot 240 can recover up to two reagents Recovered reagents will be diverted to the tubes held in the brackets on either side of the waste tank If you do not plan to recover any reagents you may set all reagent steps to Waste AFTER THE RUN Once the run is complete press the Home button to stop the rocker and return to the home screen Open the window and remove the sample tray The blot is now ready for downstream processing chemiluminescent or colorimetric detection etc The sample tray and cover can be washed using a gentle laboratory detergent Remove the reagent dispensing tubes from the machine The tubes may be discarded or washed for reuse If you choose to reuse them remove the foil seals and wash the tubes with detergent Before re use allow the tube to dry completely and seal the bottom with another foil seal Pack
10. error is that the cover or waste compartment have not been fully closed If the unit has lost power because it was unplugged or because there was a power outage simply restore power to the unit when possible If a run was in progress it will automatically re start at the point it had reached when power was lost You do not need to take any special measures If the antibodies are not being dispensed ensure that the spikes on the sample tray are facing toward the back of the instrument and that they are still sharp The BlotBot ships with several NEXT DD 8 ADVANCE Operator s Manual for the Freedom Rocker BlotBot trays Replacement trays are available for purchase If buffer is not being dispensed during the run check to make sure that the end of the buffer hose is resting in the bottom of a full bottle of buffer The bottle cap should also be slightly loose in order to allow air to flow into the bottle If the buffer supply is adequate use the Flush function to diagnose the difficulty The pumps should be audible as soon as the Fill button is pressed if they are not call customer support If the pumps are running but no liquid is dispensed make sure the buffer nozzles have not become clogged with dried salts or detergent SUPPORT FAQs protocols and other helpful information are available on our website http www nextadvance com Click on the Freedom Rocker BlotBot then on the appropriate link If yo
11. ets of tubes and seals are sold separately If you will not be using the BlotBot again for a few days you may wish to flush the buffer line with distilled water To do this place the wash buffer hose into a container of water and use the Flush function to run water through the line and into the tray MAINTENANCE Calibration The pumps that deliver wash buffer to the tray have been calibrated at the factory If you want to check or recalibrate the pumps use the Calibration function to do so From the home screen select Maintenance The on screen instructions will guide you through the calibration process which is described briefly below You will require a graduated cylinder 100 200 mL to perform the calibration Before you calibrate the pumps confirm that the wash buffer line is completely full of liquid If there are bubbles in the line use the Flush function described above to remove them NEXT DD 7 ADVANCE Operator s Manual for the Freedom Rocker BlotBot Of SS ii mrena Open the waste compartment and remove the funnel tray If you are using a larger graduated cylinder and need extra room you may remove the waste tank as well Place the graduated cylinder under the left hand buffer nozzle and press the Fill button to dispense liquid into the cylinder The pump will dispense approximately 40 mL Once the pump has stopped running measure the actual amount of li
12. ical device for humans or for any application in which the failure of the product could create a situation where personal injury or death may occur All brand and product names used in this manual are the trademarks of their respective Owners CONTACT INFO Next Advance Inc Averill Park NY USA Telephone 518 674 3510 www nextadvance com Email info nextadvance com NEXT gt gt ADVANCE
13. quid dispensed and enter the value using the encoder knob Measure the volume from the right hand nozzle the same way and record its value Press the Save button to save the values The pumps are now calibrated Replace the waste tank and funnel tray and close the waste compartment General cleaning Rinse the waste tank and funnel tray when desired The components are made from reagents that are resistant to chemicals typically used in a Western blot but some of the components of blocking buffers nonfat dry milk BSA etc can form unattractive deposits on the surface The display screen protector the work area window and the interior surfaces of the BlotBot can be wiped down as needed with water and a laboratory wipe Use of alcohol is not recommended as it may fog some of the plastic surfaces over time TROUBLESHOOTING In addition to the tips given below a list of troubleshooting tips is available at http www nextadvance com If the BlotBot doesn t start the plug of the power supply cord may not be in a live wall outlet or the power supply connector may not be fully inserted in the socket on the back of the BlotBot If the unit stops working turn the system off for one minute to allow the electronics to reset If the BlotBot does not turn on after this period contact customer service If the unit stops during a run and displays an error message the run will resume once the error is fixed The most common
14. u would like further assistance contact customer service by email at support nextadvance com or by telephone at 1 518 674 3510 SPECIFICATIONS BlotBot 120 BlotBot 240 45 cm 54 cm 17 5 in 21 5 in 36 cm 14 in 39 cm pe ass in 46 cm 43 cm melene 18 in 17 in 17 kg 15 kg Weight 38 Ib 32 Ib Capacity 4 mini blots or 1 midi or 2 midi blot blots Midi blot tray 150 mL Mini blot tray 75 mL per side Reagents dispense 2 dispense 2 recover 2 recover 2 50 mL each 50 mL each reagent Midi blot tray 7 mL 6 mL can be recovered Mini blot 3 5 mL per blot 3 mL can be recovered Protocol steps per step 5 to 40 5 to 40 independent independent for each step for each step 2 mini blots Buffer volume Minimum reagent per blot Rocks per minute NEXT DD 9 ADVANCE Operator s Manual for the Freedom Rocker BlotBot Power Requirement 12 VDC 2 1 Amp Relative Humidity 5 90 non condensing Operating Temperature 4 40 C Altitude lt 2000m Storage Temperature 40 to 50 C WARRANTY Next Advance warrants its Products against defects in materials and workmanship for time periods which vary according to the Product Within these time periods Next Advance will replace or repair without charge to the original purchaser any part which is defective BlotBot warranty Two years The warranty is void if the Product is defective due to product
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