Leica TCS SP5 Leica TCS SP5 X User Manual
Contents
1. 17 Electrical connection requirements of supply unit 17 Electrical connection requirements of achromatic light laser 17 Requirements Regarding Ambient 18 F ermitted Armbpient CorndadillOFiS sitcom dda Ro Iso esu nee 18 Waste Heat Required Cooling 19 de ete NTC Tn 19 Overview of Usable VIS UV Lasers 19 Overview of Usable MP Lasers IR 20 PICOSCCONC laser e cc 20 FeImtosecohd Ide ET nee 21 Overview of Usable VIS UV Lasers for TCS SP5 22 Which Laser Class Does the Product 23 Required Laser Safety 23 Safety Instructions and their Meanings esee 25 4 General Safety Instructions 27 4 1 Laser Class for VIS and UV 27 4 2 Laser Class for MP System uu duu O GU DRY dn Wd Y teen 27 4 3 What does the owner operator have to observe2. 27 4 4 Safety Instructions for the USer uie leap a de 29 4 5 Operational RENWADINEY iei Y ee Een ae ic 29 4 6 Maximum Current Load of the Multiple Socket Outlet at the Supply Unit 30 5 Safety Pc 31 5 1 Disconnecting the Power nenne nennen 31 5 2 Detachable key Switch u 220022400200002nnennnennnnennnn nenn nano nenne nns 32 5 3 EMISSIONS Warning Indicat r8 Ortu bte tabe to ede aA 34 5 4 Remote Interlock Connection on the Supply Unit 35 9 0 Remote interlock connection on the achromatic light laser 36 5 6 Remote Interlock Connections on External Lasers 37 5 7 Remote interlock jack interlock connector on scanner 37 5 8 Function and Position of Safety 38 5 9 Special Laser Safety 39 5 9 1 Laser protection tube and beam 39 5 9 2 Shielding in MP Systems IR 40 5 10 Safety labels on the system ae 41 5 10 1 Inverted microscope DMI 6000 41 5 10 2 Upright m
3. 81 82 Conventional MICFOSCOD a 82 WAY SCAN Eg PTT 85 How Is an Optical Section 86 Acquiring Optical Sections eee eren rn rne 88 Wate ACOUISINIOM ne a theres een 88 diced teed EP 90 esse au oui AL 91 EMISSION DANS an ee ee et 92 10 2 5 10 2 6 10 2 7 10 2 8 10 3 10 3 1 10 3 2 10 3 3 10 3 4 10 3 5 10 3 6 10 4 10 4 1 10 4 2 10 4 3 10 4 4 10 4 5 10 4 5 1 10 4 5 2 10 4 5 3 10 4 5 4 10 5 10 5 1 10 5 2 10 5 3 10 5 4 10 5 5 10 5 6 10 6 10 6 1 10 6 2 10 7 11 11 4 11 2 The Pinnole and Its EITeCIS udi ate IR EUR Fog nadie tn 93 Image Detail and Raster 95 SIG Mal ANG NOMS Cis m MP 99 miel te T ee 101 Multiparameter FIUOrESCENCE ccc cece eecceeceeceeeceeeeeeceeteeeseeceeeeeeteesaeeseesaes 102 NUMIDA UOI een ee 102 Beam SONNING De usd ED O YY 104 EFS SIOM ANG cc 104 Crosstalk ernannte een 104 SEQUENllal Scanning zen c 105 9 D RETE 105 OID SONGS nn Q 106 Z 106 Section MMICKMESSOS a a 106
4. cH 107 Data VOllmes cruce 107 GU 108 108 Mi s UUN NN 108 Orthogonal Projections biis tete vus Exec Ru 109 Rotated ade ECHO RENE 110 FARF E UU UU 110 lez iom TE 110 POINTS MO YN LO Y yn dd Yd 111 EDO me 111 UE UU UE 111 Spaces MME SDaACE E ea 111 FAP cu deed aec er ee 112 oDecttal Series ae ibi IO ee es 112 Data Acquisition and 112 About Spectral a a a 112 Combinatorial ATI al SIS isis a esta a IDEO a 112 115 GON c IL Dee 115 Cleaning the Optical System enne 115 11 3 11 4 12 12 1 12 2 13 14 15 15 1 15 2 15 3 Cleaning the Microscope 5 116 Maintaining the Scanner Cooling 116 Transport and DISPOSal UYN GWD TNG 117 Changing the Installation 117 IDDISDOS disces
5. Seica MICROSYSTEMS Published by Leica Microsystems CMS GmbH Am Friedensplatz 3 D 68165 Mannheim Germany http www leica microsystems com http www confocal microscopy com Responsible for contents Leica Microsystems CMS GmbH Copyright Leica Microsystems CMS GmbH All rights reserved Table of Contents T 1 1 1 2 2 1 2 2 2 3 2 4 2 5 2 6 2 7 2 7 1 2 7 1 1 2 7 1 2 2 7 1 3 2 7 1 4 2 7 2 2 7 2 1 2 7 2 2 2 7 3 2 7 4 2 7 5 2 8 2 8 1 2 8 2 2 8 2 1 2 8 2 2 2 8 3 2 8 4 2 8 5 3 General acs ec 9 amp 7 210 019 Diesen ten RH DUI see 9 About this Operating Manlal u a u2 ai 10 Leica TCS 5 5 TGCS SP5 X ie saa 11 TCS SP5 system 11 TCS SP5 X system 12 Meded USE ee a 12 ORION IV ne N ee 13 milite du 14 SS dr Wa o RYN a a TREE 14 SCC I erii On TTE 15 Dimensions Pe b HR RIDE 15 TCS SP5 with inverted 15 ICS SP5 with upright mletroscope Pre e e e eda 15 TCS SP5 X with inverted 16 FCS 9SP5JXwith uprghtmieroscoDG ce iE Ox b rr be Prades 16 Electrical Connection
6. Figure 20 item 2 must always be reinstalled Please consult the microscope s operating manual provided When using a condenser base with filter holder always make sure that unused filler holders are swung out of the beam path and that the laser protection tube covers the beam path When equipping multiple filter holders with filters do so from bottom to top so that the laser protection tube can cover the beam path to the greatest possible extent Do not swing in the filters during the scanning operation 39 5 9 2 Shielding in MP Systems IR Lasers The light of all employed VIS lasers wavelength range 400 700 nm visible spectrum and UV lasers wavelength range lt 400 nm invisible is fed through a fiber optic cable and therefore completely shielded until it leaves the microscope objective and reaches the specimen For systems with infrared laser wavelength range gt 700 nm the beam is passed through a safety beam guide and if necessary also passed through a fiber optic cable Figure 21 This shields the laser beam until it leaves the microscope objective and reaches the specimen Figure 21 Safety beam guide 1 and IR laser 2 40 5 10 Safety labels system The corresponding safety labels are selected dependent on laser configuration VIS UV MP and attached in the following locations either in the English or German language 5 10 1 Inverted microscope DMI 6000 CS Angled rear view of rig
7. Windows may be used in the following text without further identification It is a registered trademark of the Microsoft Corporation Otherwise no inference with regard to the free usability of product names may be drawn from the use of those names All other brand names and product names in this document are brands service marks trademarks or registered trademarks of the respective manufacturers Made in Germany Copyright Leica Microsystems CMS GmbH All rights reserved 1 2 About this Operating Manual Whenever this manual refers to the system or provides no specific information for one of the systems then the notes instructions and information applies to TCS SP5 and TCS SP5 X The main focus of this operating manual is directed to the safety notes that must be strictly adhered to when working with the Leica TCS SP5 and Leica TCS 5 5 In addition this operating manual provides a rough overview of the operating principle of laser scanning microscopes It presents you with the first steps for activating and commissioning the system and provides important information about the Leica Application Suite Advanced Fluorescence LAS AF software The system is delivered with the latest version of the licensed LAS AF To maintain information on the most current level the description of software functions was intentionally omitted from this operating manual Instead reference is made to the online help of the LAS AF in which you can
8. find up to date explanations and instructions for the corresponding software functions Please read the chapter Introduction to the LAS AF in this operating manual to familiarize yourself with its setup and operation Additional information about specific functions can then be found in the online help 10 2 Leica TCS SP5 TCS SP5 2 1 TCS SP5 system overview Figure 1 TCS SP5 system components overview TCS SP5 Scanner Main switch board TCS workstation Supply unit Control panel Microscope 11 2 2 TCS SP5 X system overview Figure 2 TCS SP5 X system components overview 1 2 3 4 5 6 7 2 3 Intended Use TCS SP5 X scanner Main switch board TCS workstation Supply unit Control panel Microscope Achromatic light laser The system was designed for confocal scanning laser scanning images of fluorescence marked living and fixed specimens as well as for quantitative measurements in the area of life science This system is intended for use in a lab Applications of in vitro diagnostics in accordance with MPG German Medical Devices Act are excluded from proper intended use 12 The manufacturer assumes no liability for damage caused or any risks arising use of the microscopes for purposes other than those for which they are intended or not using the microscopes within the specifications of Leica Microsystems CMS GmbH In such cases the Declaration of Conformity shall b
9. 10 8 Cover for Replacement Flange Front view of cover Figure 31 Cover for replacement flange If the replacement flange for transmitted light is not equipped with a functional module such as a lamp housing a cover must be placed over the opening for laser safety reasons 50
10. 5 1 and the corresponding national The owner operator is responsible for performing and monitoring suitable safety A regulations 27 All safety devices safety locks and safety systems of the laser product must be in an operational state Deactivating or damaging these safety devices or any intervention in any of these safety devices may lead to serious eye injuries physical injuries or property damage In these cases Leica Microsystems CMS GmbH shall not assume any liability The owner operator is responsible for naming a laser safety officer or a laser protection advisor according to the standard IEC EN 60825 1 Safety of laser products Part 1 Classification of systems requirements and user guidelines and the respective national regulations Repairs and servicing may only be performed by authorized Leica Microsystems CMS GmbH service personnel The owner operator is fully liable for all consequences resulting from the use of the system if it is opened improperly serviced or repaired by persons other than authorized Leica service representatives If repairs or service measures are performed that require opening parts of the housing only trained Leica service technicians may occupy the room in which the system is located Do not connect any external equipment or other components Connect to the product only those electrical devices that are listed in the operating manual Otherwise please contact your l
11. d see Figure 10 Figure 10 Detachable key switch for the internal lasers The detachable key switch for protection against unauthorized use of the external achromatic light laser is located on the front of the achromatic light laser see Figure 11 Figure 11 Detachable key switch for the achromatic light laser 32 The key switch for protection against unauthorized use of the external UV laser is located the front of the power supply see Figure 12 Figure 12 Key switch for the external UV laser For other external lasers please refer to the operating manual supplied by the laser manufacturer for the position of the detachable key switches 33 5 3 Emissions Warning Indicators The operational readiness of lasers located in the supply unit is signaled by an emission warning indicator Figure 13 The emission warning indicator is located above the detachable key switch and is yellow when lit The emission warning indicator of the achromatic light laser is located on the front of the achromatic light laser See Figure 14 and is red when lit As soon as the emission warning indicator of the lasers is lit it is possible from a functional standpoint that laser radiation is present in the specimen area Figure 13 Emission warning indicators on the main switch board Figure 15 Emission warning indicator on power supply of the external UV laser 34 Immediately disconnect the system from powe
12. designed only for the laser variants listed 2 8 2 _ Overview of Usable Lasers IR Lasers Furthermore the MP system may contain additional VIS UV lasers see the table for usable VIS UV lasers 2 8 2 1 Picosecond laser Luminous Luminous power at laser power in focal Pulse duration output W plane W pulsed 1 0 1 5 ps pulsed 1 0 1 5 ps Wavelength nm MaiTai ps 780 920 MaiTai ps wideband ps broadband Laser type Chameleon ps 690 1020 Ultra Chameleon ps 690 1040 Ultra Chameleon ps 680 1080 Ultra Il Table 2 Usable MP lasers for TCS SP5 and TCS SP5 X pulsed 1 0 1 5 ps pulsed 1 0 1 5 ps pulsed 1 0 1 5 ps 20 2 8 2 2 Femtosecond laser Luminous Luminous power at laser power in focal Pulse duration output A W Wavelength Laser type nm MaiTai 780 920 920 06 pulsed 80fs 80 pulsed 80fs CN eee pe fs pn Chameleon is 6 0 _ 1020 lt 4 lt 1 9 pulsed 140 fs Ultra Chameleon fs Chameleon fs 1080 lt 4 lt 1 9 pulsed 140 fs Ultra Chameleon 690 1040 lt 40 lt 1 9 pulsed 140 fs Vision Chameleon 680 1080 lt 4 0 lt 1 9 pulsed 140 fs Vision 680 1080 pulsed 140 fs Chameleon Compact OPO 1000 1600 pulsed gt 100 fs Table 3 Usable MP lasers for TCS SP5 and TCS SP5 X The components for laser safety are designed only for
13. e invalid 2 4 Conformity This device has been tested and meets the requirements of the following standards IEC EN 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use IEC EN 60825 1 Safety of laser products Part 1 Equipment classification requirements and user s guide Electrical Equipment for Measurement Control and Laboratory Use EMC Requirements Class A IEC EN 61326 This is a Class A instrument for use in buildings that do not include domestic premises and buildings not directly connected to a low voltage power supply network that supplies buildings used for domestic purposes Electromagnetic Compatibility EN DIN Part 3 2 Limits Limits for harmonic currents Electromagnetic Compatibility EMC IEC EN 61000 3 3 Part 3 3 Limits Limitation of voltage fluctuations and flicker in low voltage supply systems The declaration of conformity for the system is located in the appendix of this operating manual For use in the USA U S laser products Food and Drug Administration FDA CDRH 21 CFR 1040 10 Complies with FDA performance standards for laser products except for deviations pursuant to laser notice No 50 dated 26 July 2001 For the USA area of validity of the CDRH FDA the designations of the laser class are to be changed in the text from to 116 and Class 4 to IV 13 2 5 Patents The Leica TCS SP5 product is p
14. e laser safety regulations 23 24 3 Safety Instructions and their Meanings DANGER This kind of warning alerts you of an operating procedure practice condition or instruction in the operating manual that must be strictly observed and followed as otherwise you expose yourself to the risk of fatal injury WARNING LASER RADIATION A laser warning points out an operation a process a condition or an instruction that must be observed strictly to prevent serious eye injuries to the persons using the system WARNING ELECTRICAL VOLTAGE A high voltage warning points out an operation a process a condition or an instruction that must be observed strictly to prevent possible injury or death of the persons using the system WARNING HARMFUL SUBSTANCES A harmful substances warning points out a substance that can be harmful to your health CAUTION A safety instruction points out an operation a process a condition or instruction that must be observed strictly to prevent severe damage to the system or loss of data This mandatory sign draws your attention to the fact that suitable eye protection gear must be worn when commissioning and operating the system Failure to heed this warning may lead to serious and irreversible eye injuries WEARING PROTECTIVE EYEWEAR OBSERVE INSTRUCTIONS IN OPERATING MANUAL This mandatory sign draws your attention to the fact that the safety notes and regulations stipulated in the ope
15. e system will apply a static load of 200 kg m N Ensure that the environment is as vibration free as possible 2 7 4 Permitted Ambient Conditions Permissible temperature range for operation 18 to 25 C Temperature range for optimum optical 22 C 1 C behavior Permitted relative humidity 20 80 non condensing Permitted vibrations Frequency range 5 Hz 30 Hz lt 30 um s RMS Frequency range gt 30 Hz lt 60 um s RMS Pollution degree 18 2 7 5 Waste Heat Required Cooling Performance The TCS SP5 system has a maximum power consumption of 3 2 kW VIS system or 6 2 kW MP system The TCS SP5 X has a maximum power consumption of 3 4 kW For the specifications of external lasers such as UV and MP lasers please refer to the manufacturer s documentation 2 8 Features 2 8 1 Overview of Usable VIS UV Lasers The Leica TCS SP5 features a combination of the lasers listed below Maximum Maximum Wavelength luminous power luminous power at laser output in focal plane Laser type mW Pulse duration Diode 405 Continuous wave cw Continuous wave Cw Diode 405 p pulsed 60 ps 458 476 488 Continuous wave DPSS 445 458 476 488 Continuous wave Continuous wave cw Continuous wave cw Continuous wave cw Continuous wave cw Continuous wave cw Table 1 Usable lasers for TCS SP5 without MP 19 The components for laser safety are
16. ensions of TCS SP5 X with inverted microscope 2 7 1 4 TCS SP5 X with upright microscope 1490 mm 2900 mm 1200 mm Figure 7 Dimensions of TCS 5 5 X with upright microscope 16 2 7 2 Electrical Connection Requirements The building installation must feature three separate power connections with the following fuse protection 3x 100 V 120 V power supply at 20 A or 3x 200 V 240 V power supply at 12 16A For the specifications of external lasers such as UV and MP lasers please refer to the manufacturer s documentation 2 7 2 1 Electrical connection requirements of supply unit Supply voltage 100 240 V AC 10 9 2 7 2 2 Electrical connection requirements of achromatic light laser Supply voltage 100 240 V AC 10 9 Applies only to the TCS 5 5 system 17 2 7 3 Requirements Regarding Ambient Conditions A Ensure that the system is not installed next to air conditioners or ventilation systems For this reason the installation location should be carefully planned A Also read the notes on protection against dust in Chapter 11 Care and Maintenance Do not expose the system to drafts Ensure that the environment is as dust free as possible Installing the system in darkened rooms is also advisable The system requires doors with inside widths of 1 0 m for installation maintenance and transport With regard to the load bearing capacity of the floor note that th
17. ht side of microscope Figure 22 Safety label for inverted microscope DMI 6000 CS 41 Angled front view of right side of microscope 29100 017 zust 00 52005 005 zust 01 Figure 23 Safety label for inverted microscope DMI 6000 CS 42 5 10 2 Upright microscope DM 5000 6000 CS Angled front view of right side of microscope Figure 24 Safety label for upright microscope DM 5000 6000 CS 43 Rear view of microscope Figure 25 Safety label for upright microscope DM 5000 6000 CS 44 5 10 3 Scan Head Angled front view of left side of scan head D p SS vi a E D LUI A ah m d y 1 1 i r g Figure 26 Safety label for the scanner 45 5 10 4 Achromatic light laser Rear side of achromatic light laser Figure 27 Safety label on the rear side of the white light laser i Applies only to the TCS SP5 X system 46 5 10 5 External UV laser Figure 28 Safety label on external UV laser i Applies only to systems with an external UV laser 47 5 10 6 Supply Unit View of supply unit 52005 002 Figure 29 Safety label for the TCS SP 5 supply unit front side 48 5 10 7 beam coupling unit Angled front view of the right side of the MP beam coupling unit 52005 002 Figure 30 Safety label for the MP beam coupling unit top side 49 5
18. icroscope DM 5000 6000 43 5 10 3 called Y 45 5 10 4 Achromatic rc EE DO S 46 5 10 5 UN aSo ac Gae ni ei 47 5 10 6 R 48 5 10 7 MP Dear coupling rs 49 5 10 8 Cover for Replacement nenne nennen 50 5 10 9 Mirror HOUSINO nn een 51 6 Safety Instructions for Operating the System 53 6 1 Requirements Related to the Installation Storage Location 93 6 2 General Safety Instructions for 53 6 3 Eye PTOI CU OM ete 54 6 3 1 MP System with Upright 54 6 3 2 MP System with Inverted 54 6 3 3 6 4 6 5 6 6 6 7 6 8 6 9 6 10 6 10 1 7 1 7 2 7 3 9 1 9 2 9 2 1 9 2 2 9 2 3 9 3 9 3 1 9 4 10 10 1 10 1 1 10 1 2 10 1 3 10 1 4 10 2 10 2 1 10 2 2 10 2 3 10 2 4 VIS and UV Systems with Inverted or Upright Microscope 54 SBEEMEN ATS ee enn HR Y T 55 atu et Ted ad Gn datas
19. oa DG GON 56 Changing OD CS NENNT UT 57 Changing Transmitted Light Lamp 58 Mirror housing on upright 60 Changing Filter Cubes Beam Splitters or 62 Piezo focus on upright 63 Objective Change with Piezo Focus Configuration 64 Starting Up the System eu ee d 65 Switching OM ING SY STC surung e 65 LAS AF ue ee I a Beckers 70 ei ug o UP USES mE 72 SWIICHING Off the System ce sn era 73 Introduction to LAS AP noces 75 eH ee nn Eee 75 One FIC D m T Um 75 Str cture or the Heli ese en i au nme 75 Accessing the Online 76 Full text Search with Logically Connected Search 76 Structure of the graphical user 78 General Structure of the Graphical User Interface 78 Key Combinations cR 79 Introduction to Confocal 81
20. ocal Leica service agency or Leica Microsystems CMS GmbH Leica Microsystems CMS GmbH shall not be liable for damages resulting from nonobservance of the above information The above information does not in any way implicitly or explicitly modify the warranty and liability clauses contained in the general terms and conditions of Leica Microsystems CMS GmbH 28 4 4 Safety Instructions for User Read and observe the safety instructions in the operating manual and the safety labels located on the system Failure to observe the safety instructions may lead to serious injuries and to significant damages to the system and loss of data This laser product may be operated only by persons who have been instructed in The instrument is a Class 3B or 4 laser product depending on the laser used A the use of the system and the potential hazards of laser radiation Before carry out operating steps with the system for the first time first read the 9 corresponding description of the function in the online help 1 For an overview of the individual functions refer to the table of contents of the online help As it is impossible to anticipate every potential hazard please be careful and apply common sense when operating this product Observe all safety precautions relevant to Class 3B lllb lasers and Class 4 IV lasers for MP systems Do not deviate from the operating and maintenance instructions provided herein The failure to observe the
21. of the TSC SP5 X system you have to use the remote interlock jack on the supply unit see chapter 5 4 The A remote interlock plug shorting bridge must be connected to the remote interlock jack of the white light laser SP5 system you have to use the remote interlock jack on the white light laser If you operate the white light laser separately without connecting it to the TCS A see Figure 17 for connecting remote interlocks Remote interlock devices such as those connected to the room the door or other onsite safety interlock systems can also be connected to the remote interlock connector The laser beam path is interrupted if the contact is open Superk Figure 17 Remote interlock connection the achromatic light laser Applies only to the TCS SP5 X system 36 Remote Interlock Connections External Lasers 5 6 For external lasers please refer to operating manual supplied by the laser manufacturer for the position of the remote interlock connection Remote interlock jack interlock connector on the scanner 5 7 The interlock jack is located rear side of the scanner operating voltage 12 V DC Figure 18 For laser safety reasons the inverted microscope must be connected to this connection or if 37 This ensures that the safety switch of the O E os O e 6 C oo Sc gt In icrosc
22. ope is use tegrated Location of the interlock jack E o O N mu N Se 5 5 c m 5 8 Function and Position of Safety Switches When the safety switches are released the light path of the laser beam is interrupted Figure 19 Position of the transmitted light illumination arm 1 and switching from scan mode to eyepiece 2 Inverted PH IDI OOF Prevents laser light microscope DMI while working the 6000 CS Tor VOIE DIE specimen specimen Transmitted light illuminator arm Prevents stray light if changeover Inverted the user switches mirror for the between scanning microscope DMI from confocal Motorized The deflection scanner is swung observation to out by motor eyepiece observation mode and 6000 CS eyepiece 38 5 9 Special Laser Safety Equipment 5 9 1 Laser protection tube and beam stop On inverted microscopes the safety beam guide and the beam stop serve as protection against laser radiation emission and are located between the condenser base and the transmitted light detector see Figure 20 1 Laser protection tube 2 Beam stop illustrated is the version of the beam stop for MP systems 3 Condenser base Figure 20 Inverted microscope If you reorder a condenser base Figure 20 item 3 be aware that the condenser base is delivered without the beam stop Figure 20 item 2 The existing beam stop
23. r supply if any of the following occur e The emission warning indicator is not lit after being switched on using the detachable key switch A e The indicator continues to be lit after being switched off using the keyswitch e Scanning of the specimen is not activated after being switched on properly laser radiation in the specimen area Contact Leica Service immediately For other external lasers please refer to the operating manual supplied by the laser manufacturer for the position of the emission warning indicator 5 4 Remote Interlock Connection on the Supply Unit The remote interlock jack is located on the rear side of the supply unit 12 V DC operating voltage Figure 16 The remote interlock plug which contains a shorting bridge is connected to this jack Remote interlock devices such as those connected to the room the door or other onsite safety interlock systems can also be connected to the remote interlock connector The laser beam path 15 interrupted if the contact is open The overall length of the cable between the two connecting pins of the remote interlock connector must not exceed 10 m Figure 16 Remote Interlock Connection on the Supply Unit 35 5 5 Remote interlock connection on the achromatic light laser The remote interlock connection is located on the rear side of the achromatic light laser 12 V DC operating voltage see Figure 17 If the white light laser is operated as a component
24. rating manual must be observed for the secure and interference free operation of the system The operating manual in particular the safety notes must be observed by all people who are working with the system 25 Notes either contain additional information specific topic or special instructions on the handling of the product 26 4 General Safety Instructions 4 1 Laser Class for VIS and UV Systems In accordance with IEC EN 60825 1 this system is a laser product of Class Never expose eyes or skin to direct radiation The laser light can cause permanent eye damage 4 2 Laser Class for MP Systems In accordance with IEC EN 60825 1 this system is a laser product of Class 4 IV Never expose eyes or skin to direct or indirect radiation Laser light can cause permanent eye damage and skin injuries 4 3 What does the owner operator have to observe The owner operator of this product is responsible for proper and safe operation and safe maintenance of the system and for following all applicable safety regulations The owner operator is fully liable for all consequences resulting from the use of the system for any purposes other than those listed in the operating manual or the online help A This laser product may be operated only by persons who have been instructed in the use of the system and the potential hazards of laser radiation measures according to IEC EN 6082
25. rotected by the US patents 5 886 784 5 903 688 6 137 627 6 222 961 6 285 019 6 311 574 6 355 919 6 423 960 6 433 814 6 444 971 6 466 381 6 510 001 6 614 526 6 654 165 6 657 187 6 678 443 6 687 035 6 738 190 6 754 003 6 801 359 6 831 780 6 850 358 6 867 899 Further patents are pending The Leica TCS SP5 X protect by the US patents 5 086 784 5 903 688 6 137 627 6 222 961 6 285 019 6 311 574 6 355 919 6 423 960 6 433 814 6 444 971 6 466 381 6 510 001 6 567 164 6 611 643 6 614 526 6 654 165 6 654 166 6 657 187 6 678 443 6 687 035 6 710 918 6 738 190 6 754 003 6 796 699 6 801 359 6 806 953 6 831 780 6 850 358 6 867 899 6 888 674 6 898 367 6 958 858 6 961 124 7 005 654 7 092 086 7 110 645 7 123 408 Further patents are pending 2 6 Serial Number The serial number of your system is located on the rear side of the scanner oo oo ao 58 Figure 3 Rear side of scanner label with serial number 14 2 7 Specifications 2 7 1 Dimensions 2 7 1 1 TCS SP5 with inverted microscope 1400 mm 600 mm 730 mm f Figure 4 Dimensions of TCS SP5 with inverted microscope 2 7 1 2 TCS SP5 with upright microscope 1490 mm 2400 mm 1200 mm Figure 5 Dimensions of TCS SP5 with upright microscope 15 2 7 1 3 TCS 5 5 with inverted microscope 1400 mm 3040 mm Figure 6 Dim
26. se instructions shall be exclusively at the operator s own risk and may void the warranty 4 5 Operational Reliability This instrument must not be used together with life support systems such as those found in intensive care wards This instrument may only be used with a grounded AC power supply Contact with liquids or the entry of liquids into the housing must be avoided 29 4 6 Maximum Current Load of the Multiple Socket Outlet the Supply Unit The total power consumption of all loads connected to the multiple socket outlet Figure 8 must not exceed 800 VA The terminals are intended for TCS workstation e Monitor 1 e Monitor 2 e Microscope Figure 8 Multiple socket outlet rear side of supply unit 30 5 Safety Devices 5 1 Disconnecting the Power Supply The main circuit breaker is located on the right rear side of the supply unit It is used to de energize the complete system using a single switch Figure 9 The main circuit breaker functions as a switch and as an overcurrent fuse The main circuit breaker is not to be used as the regular on off switch for the system The supply unit must be set up so that the main circuit breaker is freely accessible at all times Figure 9 Supply unit with main circuit breaker 31 5 2 Detachable key Switch The detachable key switch for protection against unauthorized use of the laser products is located on the main switch boar
27. sit takers 117 MI E M UE MI 117 GIOSS aU lg LIMINE MINI eee 118 ADDODODCsmutacaunc Hed lI I MM MINI 124 Safety Data Sheets from Third party Manufacturers 124 Deelaratlon Or Gontor eset LEER 129 People s Republic of iiie rises tueri cv be a a 130 1 General 1 1 Copyright The instructions contained in the following documentation reflect state of the art technology and knowledge standards We have compiled the texts and illustrations as accurately as possible Nevertheless no liability may be assumed for the accuracy of this manual s contents If you have any comments on this operating manual or on any of our other documentation we would be pleased to hear from you The information contained in this Operating manual is subject to change without prior notice All rights to this documentation are held by Leica Microsystems CMS GmbH Adaptation translation and reproduction of text or illustrations in whole or in part by print photocopy microfilm or other method including electronic systems is not allowed without express written permission from Leica Microsystems CMS GmbH Programs such as LAS and LAS are protected by copyright laws All rights reserved Reproduction adaptation or translation of these programs is prohibited without prior written permission from Leica Microsystems CMS GmbH The term
28. the laser variants listed 21 2 8 3 Overview of Usable VIS UV Lasers for TCS SP5 The Leica TCS SP5 X features a combination of the lasers listed below Maximum Maximum Wavelength luminous power luminous power at laser output in focal plane Laser type mW Pulse duration Diode 409 cw Diode 405 p pulsed 60 ps power 55 458 476 488 Continuous wave laser cw Achromatic 170670 lt 500 lt 50 Pulsed light laser Table 4 Usable lasers for TCS SP5 X The components for laser safety are designed only for the laser variants listed 22 2 8 4 Which Laser Class Does Product Have Wavelength range Configuration variant 400 700 nm visible laser radiation 350 1600 nm visible and invisible laser radiation 350 700 nm UV visible and invisible laser radiation 2 8 5 Combination of lasers from Chapter 2 8 3 or 2 8 1 without lasers having wavelengths of 350 400 nm 3B IIIb 3B IIIb A IV Combination of lasers from Chapter 2 8 3 or 2 8 1 VIS and UV lasers Combination of lasers from chapter 2 8 1 VIS UV Laser chapter 2 8 2 IR Laser or chapter C2 8 3 VIS UV Laser Required Laser Safety Measures Please observe the laser safety measures for laser class 3B VIS and UV systems laser class 4 IV MP systems in accordance with applicable national and federal regulations The owner operator is responsible for observing th
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