MYECL™ Imager Manual
Contents
1. TM Thermo SCIENTIFIC Table of Contents Chapter 1 OVerVieW ss 1 Thank You and Welcome Letter RR RR 1 Contacting Thermo Fisher Scientific sssr 2 Instrument OVervleW tes ere eeeseskdse ien eens Ne daerees Os bees eek dk Gees 2 Included seed EE 2 Functional y EE N EE EE N EE 3 MYECL Imager ACQUISITION SOftWAaTe eesesse sesse ee se se eene Se ee RR GR Gee ee ee 3 vmylmageAnalysis Software ccccssssssssssssssssssssseessssssssssesesssssssssecsssssssssssseennecsssssnseeee 4 Chapter 2 IMA GCP ccsccsssnnsnntninstnnnnnnnnsnnnntnasenasnese 5 Installation Guidelines ee REK EE 5 Space Reauieme SE GENE EN Ee NN Ed ee ooN 5 Electrical Reotitem RS sees KEER SE EER GR RE GN RAAR DRR ee 5 Environmental Requirements ss rd ei N se dei ee ee 6 Computer Requirements cscssecsssssssscsscsessesserscssscssessessersessecsseseerseseesesees 6 Instrument Images with Feature Locations esse sesse sesse SR SR ee 7 UG EE EE EE OE OE Pd 10 op Bi Be id EE ON 11 Optional USB FeatUF S RR 12 Instrument Settings 0c ccccsssssssssseeseccccssssescesssssssssssssesssececccsssseseessssssssen 13 tels EE EE EE EE S E OE EE ERT 13 Date nd TIMO ee ee ei fees tures oe oe RE os De id 14 BED BA OE EE OE EE 15 so EE EE 16 Administrator Set idS SE N EE EE Ee ee 18 Set Image Gallery Location se ES OE tasted 18 Allow Remote AEEBSS ee ed oes Rg ee Geo ee Gee ei 19 Other Operating System OS Settings esse sesse se se see se ee ee Ge Se ee 20 Reset Default2. When complete touch the Save button at the bottom of the menu to save the changed defaults Filter Menu Selections Position Description Wavelength No Filter Chemi Chemi Orange 545 630nm Green 515 560nm Blue 445 480nm Save Red 620 645nm Table 2 4 Imager filter specifications and corresponding dyes Filter Color Wavelength Range Compatible Dyes Sample Screen nm and Stains Blue 445 480 DAPI Clear screen AMCA Clear screen Green 915 560 SYBR Green Clear screen SYBR Gold Clear screen Orange 945 630 Ethidium Bromide Clear screen Hoechst Blue Clear screen Coomassie Blue White screen Imperial White screen Red 620 645 SYPRO Ruby Clear screen Deep Purple Clear screen S With the MYECL Imager the orange filter is optimal for Hoechst Blue imaging t Coomassie Blue and Thermo Scientific Imperial Stains do not require the orange filter however these stains require the white light conversion screen UV and Vis modes use the orange filter in Position 2 by default 17 MYECL Imager User Manual Thermo Fisher Scientific 18 Administrator Settings Set Image Gallery Location MYECL Imager User Manual Users will typically not need to access Administrator Settings Only the facility administrator should have access to these functions through password access The default user name and password are myecl and 1234 respectively To avoid improper access the designated administrator need
3. FOCUS Position asie ies idee ee ee n Ee Ee ee 20 Meel ets OTN soo OE ONE OE NE N OE OE ET N 20 Change Username and Password ssssssssssssssssssssssscsesssssssssssssssseseaeers 20 BEd RE N N Ee ON EE OE EE EE N ON 20 Chapter 3 Image Acquisition 21 Imager of HEES ee GE Ge Oe 21 Camera Settings Available in Chemi UV and Vis Mode 22 Settings Only Available in UV and Vis Mode se sesse se se see ee se se 24 Chemiluminescence CHEMI Mode 25 Camera o WINGS oe GE OE ee 25 TS el ES EE EE Oe Ee ee de GE Oe 25 Se 01a ves E OR A E N 25 Pre set Exposure TIME sesse esse ee ee ee ee ee ee ee ee ee ee ee ee ee ee Ge ee ee ee ee 26 US ede gee Ii EE a aa eriari 2 Multiple Image Acquisito AR EE GO O sN Piki 28 Pre set Multiple Exposure Settings ees esse ese ee ee ee ee ee ee ee ee ee ee ee 29 User defined Multiple Exposure Settings esse esse esse ese ee ee ee ee ee ee ee ee 30 Haage TONS ME OR OE AE OE EL OE N E 32 BEE Sati ele EE EEE 32 Te AE ER EE EE Ee N ed 33 Pete EO AE OE ee ee NE Ve ee N ee RE N ee 33 Ee de ele Ee RE Ee 33 Ultraviolet UV Mode ER EE IE ER EE Ee 34 Camera S ttinS cscssssscscscececssersesssscscecessesesscsescecessnsesscesacsescesessesarseseececes 34 Capture Setin EE N EE EE EE EE N 34 Miage CO AE EE OE EO EE OIE EO EN IE 34 Pre set Exposure TIME se ee DE GO Ie N N GE Ge 35 Custom Exposure N OE OE N 36 Stop Image CCG OO oe ee ee ee Ne OE ee Ne GE EE ee ee 37 Voor e se AE EE rey
4. Vis mode is used for the capture of gel images using reflected and or transmitted light CAUTION Vis mode uses the UV lights from the transilluminator Long term exposure to UV light can be harmful Use the appropriate eye and skin PPE if there is any chance of exposure to the UV light Note The transilluminator UV light is initially on for 5 minutes User input will then be requested for further use of the UV light If you do not select the Keep UV on button after the initial 5 minutes then the UV light will automatically turn off Vis mode uses the same functional camera settings as the UV mode 1 Select the Vis tab on the touchscreen Changing to Vis mode automatically turns on the UV lights within the transilluminator and the LEDs 2 Optional Select the Live Camera button and enable the UV button if the live image is not displayed and the transmitted light is off 3 Open the imager door confirm the UV light is off pull out the transilluminator and install the white light conversion screen on top of the transilluminator 4 Insert and center the sample on top of the white light conversion screen using the live image display for reference 5 Once the sample is centered close the imager door The UV lights will turn on and show as visible light transmitting through the white light conversion screen 6 While viewing the live image adjust the necessary settings for Focus Digital Zoom Sensitivity Resolution and Apertu
5. imager weighs 65lbs 29 5kg Please use two people to lift it by grasping the bottom and the handle at the rear of the imager Using two people place the imager onto the benchtop given proper space electrical and environmental requirements Remove the outer packaging materials IMPORTANT Imager use outside of the workflows described in this manual may put the operator at risk of dangerous exposure to electrical shock or radiated emissions Do not use this instrument for any purposes or in any configurations not described in this manual Thermo Fisher Scientific 2 Contacting Us Imager Overview Included Accessories MYECL Imager User Manual For technical or service assistance on the MYECL Imager e U S Customers 1 800 874 3723 e Fax 1 800 842 5007 e International Customers 1 815 968 0747 e Fax 1 815 968 4736 Technical Support email for U S and international customers pierce ts thermofisher com Technical Support email for European customers perbio eurotech thermofisher com For more information about the Thermo Scientific Protein Biology product line please visit our website www thermoscientific com pierce The MYECL Imager is designed to be a useful tool for the researcher needing intuitive equipment and software with fast imaging capabilities Your imager purchase includes the following accessories each quantity 1 Thermo Scientific MylmageAnalysis Software CD and User Manual Chemiluminescence exposu
6. you dont have a USB printer Windows automatically installs USB printers when you plug them in gt Add a network wireless or Bluetooth printer Make sure that your computer is connected to the network or that your Bluetooth or wireless printer is turned on 7 items Thermo Fisher Scientific MYECL Imager User Manual Filters The imager has four default filter positions Position 1 No filter for use in Chemi applications Position 2 Orange filter for use in UV and Vis applications Position 3 No filter Position 4 No filter The imager is supplied with an orange filter for Position 2 but green red and blue filters can also be purchased for additional applications You can install these filters into Positions 3 and 4 depending on your position preference The Filter Menu Selections in Tools allow you to assign the filters for each of the Positions 3 and 4 when different filters are installed 1 Touch the Filters button The following menu will appear Thermo 2 Chemi EE UV Vis HE Gallery 9 F Serial Number KR01211106 Filter Menu Selections Position Description Wavelength No Filter Orange 545 630nm C Green 515 560nm O Blue 445 480nm Save Red 620 645nm 2 Positions 2 3 and 4 use selectable drop down menus to enable you to choose between Empty Orange Green Blue and Red Select the choice for each filter position Thermo Fisher Scientific 3
7. 37 yi Stes SALUT lee EE EAEE EO EE EAA E 37 PASE NAGS EO OE N TO OE OE N 38 PUES CONAN SS OE GE ee OD ee 38 Visible Mis Mode ae ER oe ee 39 Camera Settings EE Ee Ge Ge Ee GE ee 39 Image Aeduisiti n transmitted Light sesse eens dese di ee see en deed Sie 39 Image Acduisition Reflected Light ss ses oe ED RE PER GO GES N SEN GN eN GEE 39 Pre set Exposure TME essensie ERNA ee GR ed ende de Ee sia kears ranri 40 Custom Exposure TMe os Ee GE EN Ge RE IS GR N ee Ee ee 41 WIN ANS TOONS een 41 Display eie ee AR ESN 41 PSEC T ED AET 42 Auto Conta EE eN N 43 File MAMA CIN ONE us GE EE De ED 43 AGS OO sarees OE iris runnin tienen 43 EOIN AE etensntesscaeemineartstetiansae 43 Sy EO EE EO EE EE N 43 Bi AE OE EE N EAA E 43 Vaste Alg edge eg EE EO EE N GE N 44 Image Gallery OR EE EE EE EE 45 Ee VRS AE EO ee Ee 45 UT AGA n RE EE EE Ee Oe eN 45 SES EE O 46 STE el ee ee A enter erate Tey EE IN 46 Chapter 4 Maintenance 47 Cleaning the Imager and Sample ScreenS u s 47 LCD Touchscreen REKE 47 SS ON tie SES E R ERE 47 UV TiS UT niie ti Gl eo EE OE EE oe Ie 48 Slp ode ee AE acoesscuenaesssnes eabsoseosedvaserastavaessesaeaysaavidexennisinasissatest 48 Cleaning the TransillumMinator ss ssessesssessessesseessessesseesserseessesseeseessessesse 49 Replacement of Transilluminator BulDS eeesesesese se se see eek Re ee ee 49 Emission Filters ers aie Se Oe oe Ge ee Ee ee 50 Sleg ede LA OR EE EO EE N N 50 Appe
8. A pe consizabon Power phor A Prog ans are sur es D hase HO Audo Manager d Roser Piny and Language E hemoteige and Desitop Connectors yaler ad Tost 1 Mer N Troudieshocknsg M Uver As P Wired Feed T Wirrdoes Lipciste The default focus position can be reset or changed by selecting the Reset Default Focus Position button The focus position may need resetting if the current default focus position does not provide in focus images The administrator can control this position by going to Tools gt Administrator Settings gt Reset Default Focus Position and using the slider bar to focus in or out to achieve the required sharpness in Chemi UV and Vis modes This function installs and updates the acquisition software when a program update becomes available This function allows the administrator to change the username and password for accessing the administrator settings Touching Return will bring the administrator back to the previous Tools menu Thermo Fisher Scientific Chapter3 Image Acquisition Imager Settings Upon powering up the imager the MYECL Imager welcome screen will display Welcome to Thermo Scientific myECL Imager 2012 Thermo Fisher Scientific inc All rights reserved Al trademarks h erm O ente Inc and ts subsdangs are the property of Thermo Fisher Scienti Note If hard powered off the thermoelectric camera cooler will NOT be at the ideal temperature of 25 C Af
9. N Date r Wednesday May 02 2012 KRO1 211106 le L S 7 j Time ae vA 1 02 49 PM Ge Change date and time Time zone aiaa UTC 05 00 Eastern Time US amp Canada Change time zone Daylight Saving Time ends on Sunday November 04 2012 at 2 00 AM The clock is set to go back 1 hour at that time I Notify me when the clock changes Get more time zone information online How do I set the clock and time zone Administrator Settings Thermo Fisher Scientific 15 MYECL Imager User Manual Printer To select a default printer for imager exporting 1 Touch the Printer button A Printers and Other Devices screen will display 2 Choose the appropriate printer from the listed selections Plug and play printers will automatically populate the list Select Add a Printer from the left column if the printer is not a local printer or plug and play type A network printer can also be selected as a default printer Note Consult your facility s IT department for assistance in setting up network printers Thermo Chemi RM uv Vis ER Gallery Goo ie Devices and Printers ER slof x C K j zA v Control Panel All Control Panel Items Devices and Printers X Search Devices and Printers 2 Add a device Add a printer v Devices 4 x a b k J Add Printer 4 7 What type of printer do you want to install Add a local printer a Pr Use this option only if
10. al drive connected to the imager When connecting an external hard drive to the imager we recommend changing that drive label to a letter other than C D or E Upper USB Part Figure 2 4 Location of lower USB ports 12 MYECL Imager User Manual Thermo Fisher Scientific Imager Settings The Tools button in the top right corner of the acquisition software screen gives you access to basic imager settings and functionality Tools 1 Touch the Tools button to bring up the Tools screen verter chemi Ed uv Vis T Gallery Tools button Serial Number KRO1211106 Printer Administrator Settings Large buttons for Date and Time Printer Filters and Administrator Settings will display on the screen Select the appropriate button to enter setup for each item Note Administrator Settings will open with the following screen requiring a User Name and Password Only the designated Administrator at your facility can access this section r User Name Password 13 MYECL Imager User Manual Thermo Fisher Scientific 14 Date and Time MYECL Imager User Manual To correct the date time or time zone 1 Touch the Date and Time button A Windows clock and calendar will display 2 Choose the correct settings as needed 3 Select the Apply button followed by the OK button to save the settings Date and Ta x x Date and Time Additional Clocks Internet Time Oo Ed Gallery
11. button appears over the keypad once the Acquire button is touched Image acquisition can be stopped by touching the Stop button 40 MYEGL Imager User Manual Thermo Fisher Scientific Custom Exposure Time 1 Select the Custom exposure button using the keypad 2 Enter the desired time using the Keypad numbers The m box is used to input seconds and or minutes and is active by default box is gray in color For a custom exposure time of lt 1 second select the ms box to input milliseconds The shortest exposure time that can be entered is 10 milliseconds this appears as 10 in the ms box mes ms 1 2 3 4 5 6 F 8 9 Acquire 0 Cancel 3 Once a value is entered into the Custom time field the Acquire button will become active Select the Acquire button to capture an image 4 Captured images are displayed on the screen and automatically stored in the Gallery for further viewing and selection Note A Stop button appears over the keypad once the Acquire button is touched Image acquisition can be stopped by touching the Stop button Image Tools Note The Image Tools only function while viewing or printing the image Display Saturation Invert and Auto Contrast adjustments are NOT saved with the image file Display Saturation In Vis mode while viewing the live image or after capturing an image the Display Saturation button can be selected to highlight live and captured pixels that are considered to be saturated A red false col
12. ckly restart the imager without having to wait for the camera temperature to re equilibrate 1 Turn on the front soft power switch The computer restarts and the touchscreen initiates with the default Chemi screen 2 Begin normal operation of the imager Note For extended periods of inactivity the imager can be hard powered off however for typical daily use optimal camera temperature and best image quality avoid hard powering on and off the imager Hard shut down is done with the hard power switch on the rear of the imager This turns off all power to the imager including the computer and camera cooler 1 Follow the Soft Shut Down procedure 2 Turn off the hard power switch at the rear of the imager soft shut down is done using only the front soft power switch 1 Save any current image analyses 2 safely remove any USB flash drives 3 Turn off the soft power switch 4 The computer and touchscreen will shut down and turn off The fans will continue running as needed The camera cooler will remain powered and continue to maintain the camera at the optimal 25 C 5 The transilluminator and LEDs turn off Thermo Fisher Scientific USB Features The three external USB ports on the right side of the imager can be used for flash drives which can be connected through any of the USB ports for easy file management Note The Export button always exports files to the E drive which is automatically the first addition
13. com thermoscientific com perbio For other regions visit thermoscientific com piercedistributors United States Tel 815 968 0747 or 800 874 3723 Customer Assistance E mail Pierce CS thermofisher com thermoscientific com pierce Therm SCIENTIFIC Part of Thermo Fisher Scientific
14. di 45 MYECL Imager User Manual Thermo Fisher Scientific 46 MYECL Imager User Manual 4 Choose an image or series of images by touching the images on the touchscreen Note Before selecting an image you can choose to view only the Chemi image or both the Chemi and ChemiV images of the same sample by selecting the Open ChemiV Image button at the bottom right of the Gallery screen This function is useful for establishing band locations in a Chemi image against known protein weight markers in a ChemiV image Note Selecting one ChemiM image will open all of the images associated with that specific multiple exposure Up to six images including ChemiV images can be selected and displayed at one time File List The File List tab displays image information in a list format for ease of searching and sorting The list is sortable in eight different fields by selecting the appropriate column heading File Name Date Notes Mode Exposure Project User and Resolution c igawteric Thermo ET Chemi EE UV ET Vis TT Gallery 6 C2 Thurnbrad intet i Search Search Resull Rack to Gallery ory Chem 10s 201207 1519 MOM MOT IM 319 PM ge 103 Brrang iri G 4 y y a vis ae 52 WIAT VIS PM Vie 200m Bering tx DT 11 PM uv tne Brew ixt Vig Dens WDT 1519 4 MOT N T19 PM Vie Orns Brew txt UV 200m 20920202 1MU 20120200 1 43 PM Uv Om Brag txt Vis 20m 201 2077 1519 JU 2A is IN PM vis Vm rreg tat AN Vie 200ms 519 3 12000 319 C
15. ed light Visible Vis mode for image acquisition of LED and or UV converted white light 3 MYECL Imager User Manual Thermo Fisher Scientific 4 MYlmageAnalysis Software MYECL Imager User Manual The mylmageAnalysis Software is supplied with the MYECL Imager as a vital tool for extracting information from your captured images The analysis software features Indentify lanes and bands automatically View and compare up to six images simultaneously Open multiple image formats e g tif jpeg png etc Export images and data reports directly to Microsoft Excel M Word and PowerPoint software for further analysis and presentations Analyze molecular weight determine the R and molecular weight of protein or nucleic acid bands using installed or custom added molecular weight markers Edit digital images image editing tools include contrast adjustment image rotation cropping de speckling false color application and text annotation Calculate sample purity automatically based on band and lane intensity Perform lane profile densitometry Quantitate bands or regions relatively or absolutely Acquired images are saved and exported as tif files which can be directly opened in the analysis software Thermo Fisher Scientific 5 Installation Guidelines Space Requirements Electrical Requirements A MYECL Imager User Manual Chapter 2 Imager IMPORTANT Review and implement guidelines fo
16. er User Manual Thermo Fisher Scientific 3 To install an emission filter insert the threaded side of the filter into an open port on the filter wheel Turn clockwise to screw the filter in place until hand tight 4 To remove an emission filter grasp the outer edge of the filter housing and turn counterclockwise to unscrew and remove 5 When filter installation removal is complete turn the system power on 6 Update the filter settings in the Tools menu See Chapter 2 Imager for directions on changing the filter settings o1 MYEGL Imager User Manual Thermo Fisher Scientific 52 MYECL Imager User Manual Appendix A Technical Specifications Imager Parameter Power Maximum Frequency Range AC Voltage Current Fuse Dimensions Weight Wavelength Range Camera Lens Filter Wheel Touchscreen Light Source Manufacturer Specification 120W 50 60Hz 100 240V 1 2A maximum dependent on supply voltage T4AL 250V 4A fuse 21 inches H x 12 25 inches W x 22 inches D 53 3cm H x 31 1cm W x 55 9cm D 65lbs 29 5kg Specific wavelengths between 370 700nm High resolution thermoelectrically regulated at 25 C 4 2MP digital camera with 16 bit dynamic range 50mm f 0 95 aperture 4 position motorized 10 4 Inch LCD screen 306nm trans UV epi white Thermo Fisher Scientific 3747 N Meridian Rd Rockford IL 61105 0117 USA Thermo Fisher Scientific MYECL Imager User Manual Appendix B Accessories Pr
17. er the time keypad The inverted image can be used for acquiring images or switched back to the Standard image by again selecting the Invert button EER EER ayy _ N od EE ad Original Vis Image Inverted Vis Image Thermo Fisher Scientific Auto Contrast File Management Image Info Export Print Delete 43 MmyYECL Imager User Manual selecting the Auto Contrast button increases the contrast between most bands and the background to increase visibility This adjustment can be used only for touchscreen viewing or printing of the image The contrast adjustment is not saved with the image file Image file management includes editing image info exporting printing and deleting files All image files and image information are saved and exported in individual folders These folders contain both the xml file with the image information and the tiff file containing the image Image Info Export Print Image Info can be selected for any captured image This lists the acquisition information for the highlighted image You can select Export and save highlighted images to a USB flash drive available through connection to a USB port Note Users may need to momentarily wait for the imager to detect the external drive once it is connected to the USB port for the first time The Print button allows the highlighted image to be printed to a selected external printer A Print Preview window will appear before printi
18. f the stylus to lightly tap the touchscreen for selection of menus icons and buttons Note Fingertips can be used for interaction with the touchscreen however the stylus is easier to use with gloved hands and prevents fingerprints on the screen Af MYEGCL Imager User Manual Thermo Fisher Scientific 48 UV Transilluminator 2 AA Band Excision MYECL Imager User Manual CAUTION The UV transilluminator contains UV bulbs Long term exposure to UV light can be harmful Use the appropriate eye and skin personal protective equipment PPE if there is any chance of exposure to the UV light 1 Wearing UV protective eyewear and clothing open the imager door and slide out the transilluminator 2 Place the UV exposure screen clear on the transilluminator and place the gel Sample on top of the screen IMPORTANT Use of the UV exposure screen clear protects the transilluminator from nicks and scratches as a result of excision 3 In UV mode select the UV button for the UV light A message will appear asking if you want to turn on the UV light with the door open Select Yes 4 The software then displays a reminder message requiring the appropriate PPE and asks the user to pull out the white door peg Extended UV on position Retracted UV off position 5 Excise the appropriate bands from the gel sample Note As a safety precaution the UV light automatically turns off after five minutes With 30 seconds remain
19. h an OK button and the message Thank you for selecting the Thermo Scientific MYECL Imager You are now ready to begin Touch the OK button to continue 4 You will then be prompted to enter the current date time and time zone Upon completion the screen will automatically move to the default Chemi acquire screen Thermo 2 chemi FE uv vis FM Gallery 6 OQ _ Date and Time Date and Time Additional Clocks Internet Time Date Friday February 17 2012 j Time 9 54 36 AM N Change date and time Time zone UTC 06 00 Central Time US amp Canada Change time zone Daylight Saving Time begins on Sunday March 11 2012 at 2 00 AM The clock is set to go forward 1 hour at that time Notify me when the clock changes Get more time zone information online How do set the clock and time zone cn Thermo Fisher Scientific 11 Soft Start Up Shut Down Hard Shut Down Soft Shut Down MYECL Imager User Manual lf the imager is powered down with a soft shut down then a soft start up can be done using the front soft power switch The soft power switch is the blue lighted round rocker switch located just above the front door and below the touchscreen This switch allows you to power down the imager computer and touchscreen while maintaining power to the camera cooling unit This feature allows you to power down the imager but once needed qui
20. hemi ts 201 20731 1519 201207 G1 319 Pa Clear is Brag txt UV 200s 208 20207 1343_35 208 MOOT 1 43 PM Uv MOnt Breeg tt Open lt ChemiV Search The Search tab allows you to search through the full gallery Images can be searched by File Name Date Notes Project or User by entering the needed information using the attached keyboard The search results are displayed in the search Result window the results listings are sortable by field Thermo Fisher Scientific Chapter 4 Maintenance Cleaning the Imager IMPORTANT When using any cleaning products with the imager consult the appropriate material safety data sheet MSDS to confirm compatibility with the and sample Screens imager and proper usage of the cleaning product Spray a soft cloth or paper towel with cleaning solution e g general purpose cleaner isopropanol or 70 ethanol Wipe the surface of the transilluminator the sample screens and the imager s exterior housing LCD Touchscreen Use a pre moistened LCD screen cleaning tissue to clean the surface of the touchscreen Cleaning the Touchscreen IMPORTANT Use of ammonia based cleaners to clean the touchscreen is NOT recommended Do not spray cleaner directly onto the LCD screen IMPORTANT Do not press hard while cleaning the screen Do not leave excess cleaning solution on the LCD screen as this may cause long term damage Use of the Stylus The stylus is recommended for interacting with the touchscreen Use the tip o
21. hes x mm x mm um x um inch inches t 3 x 3 binning in 16 bit grayscale tiff image files are the default settings for the imager Default Button used to reset all imager settings to their default values Live Image Button used to activate or close the camera s live view of the image area 23 MYECL Imager User Manual Thermo Fisher Scientific 24 Settings Only Available in UV and Vis Modes MYECL Imager User Manual Camera Settings QQ In Focus Out CO I gt Out Digital Zoom In ol lt gt Open Aperture Close iili gt Orange 545 630nm la sl uv Default Aperture Slider bar for adjusting the camera lens opening to control sensitivity and depth of field The aperture can be fully closed far right position or fully opened far left position depending on the light sensitivity needed UV Light Button for turning on off the transilluminator UV bulbs Filter Wheel Position Drop down menu for moving the filter wheel position to switch filters Note Filter Wheel Positions 2 4 can be set for different filter colors by accessing Tools gt Filters amp Orange 545 630n m 5 i 1 Chemi O 2 Orange 545 630nm O 3 Green 515 560nm 4 Red 620 645nm Display Saturatic Thermo Fisher Scientific 20 Chemiluminescence Chemi Mode Camera Settings Capture Settings Single Image Acquisition MYECL Imager User Manual Chemiluminescence Chemi Mode
22. iding pins into the bulb socket and rotating 90 clockwise A click will be heard when the bulb pins are set Repeat Steps 5 and 6 for the remaining five bulbs Replace all necessary components of the transilluminator and avoid damaging the electrical cord Replace and hand tighten the four thumbscrews Push the transilluminator back into the imager 10 Plug the imager power cord back in and turn the system power on Thermo Fisher Scientific Emission Filters A variety of emission filters can be used in the imager The appropriate filter choice is dependent on the emission wavelength of the sample Consult Table 2 4 in Chapter 2 to confirm your filter choice Changing Filters The filter wheel allows for easy installation and removal by using threaded filters The filters are fragile so use caution when handling and installing or removing IMPORTANT When installing filters ensure that the filter threads are correctly aligned with the filter wheel threads Forcing a filter to screw in will result in cross threading and damage to the filter wheel 1 Turn off the system power 2 Open the imager door and locate the filter wheel at the rear of the cabinet Each filter position is labeled with a red position number Position 1 Permanently empty for chemiluminescent imaging Position 2 Orange filter default filter supplied with the imager Position 3 Open for additional filter Position 4 Open for additional filter 50 MYECL Imag
23. ightness and Saturation 4 If required the image exposure time may need to be increased or decreased to acquire a properly saturated image Exposure time can be adjusted before image capture by using the number keypad 5 Select the Display Saturation button again to turn off the false color imaging Note The Display Saturation button is disabled when the Auto Contrast function is enabled 6 Proceed with the steps for acquiring an image Thermo Fisher Scientific Invert Image The Invert button is a useful tool for viewing faint gel bands This tool inverts the Standard black background with white highlights to a white background with black highlights ma mr EE em am hal aan us an afi e ma i Original UV Image Inverted UV Image 1 In the active live image select the Invert button from under the time keypad 2 The inverted image can be switched back to the standard image by again selecting the Invert button Auto Contrast Selecting the Auto Contrast button increases the contrast between the signal and the background to increase visibility This adjustment can be used only for touchscreen viewing or printing of the image The contrast adjustment is not saved with the image file 38 MYECL Imager User Manual Thermo Fisher Scientific 39 Visible Vis Mode 2 AA Camera Settings Image Acquisition Transmitted Light Image Acquisition Reflected Light MYECL Imager User Manual Visible
24. ing a message will appear on the touchscreen indicating that the UV light will be turned off unless the Keep UV on button is selected Thermo Fisher Scientific 49 Cleaning the Transilluminator A Replacement of Transilluminator Bulbs MYECL Imager User Manual 6 When finished turn off the UV light by pushing in the white door peg Push the transilluminator back into the imager Alternatively push the transilluminator back into the imager and close the door which automatically pushes the white door peg back into place Ensure the transilluminator surface is cool Saturate a clean soft cloth or paper towel with cleaning solution e g general purpose cleaner isopropanol or 70 ethanol Wipe the surface of the transilluminator Note To ensure uniform illumination replace all six UV transilluminator bulbs even if only one bulb fails 1 2 9 Shut down the imager and unplug the power cord Open the imager door and completely slide out the transilluminator Ensure the transilluminator is cool to touch Remove the four thumbscrews on the transilluminator coverplate Lift the coverplate to expose the bulbs Use caution to avoid damaging the electrical cord Remove the transilluminator bulbs by rotating 90 counterclockwise until the pins align with the bulb socket A click will be heard when the bulb pins are aligned Lift the bulb up and out of the socket Insert the new transilluminator bulbs by sl
25. is the default mode Chemi mode is used for the capture of sample images using sample emitted light These images of the blot are useful for imaging colorimetric molecular weight markers Focus Digital Zoom and Resolution Sensitivity camera settings are available in Chemi mode Select the Chemi tab on the touchscreen Optional Select the Live Camera button if the live image is not displayed The white LED lights and live image display are activated Open the imager door pull out the transilluminator and install the chemiluminescence exposure screen black on the transilluminator glass Insert and center the sample on top of the chemiluminescence exposure screen black using the live image display for reference Close the imager door While viewing the live image adjust the settings for Focus Digital Zoom and Resolution Sensitivity in the Camera Settings if needed Note Camera Settings are available only with the live image display select a Pre set or Custom exposure time Note ChemiV images are captured and automatically saved to the Gallery however they are not displayed in the image tab if Live Camera is not enabled before image acquisition in Chemi mode Saved ChemiV images can be displayed in Gallery mode along with the Chemi images Thermo Fisher Scientific 26 Pre set Exposure Time MYECL Imager User Manual 1 Select one of the pre set exposure time buttons on the touchscreen keypad The imager take
26. k the red button to Edit an entry Exposure 1 2 3 4 5 Total 1 Select the Multi button from the touchscreen number keypad 2 To create or edit the user defined exposure time settings select the Edit button to highlight the five Exposure fields 3 Choose one of the User option buttons to highlight the set of cells for user input 4 Using the time keypad input the desired time into each cell Enter exposure times in ascending or increasing increments with the shortest amount of time in column 1 and the longest amount of time in column 5 The highlighted cell will move to the right as each time is input User defined Save Tap the Save button first then click the button to capture Exposure 1 2 3 4 5 Total Lo i 5 The Total time is calculated and displayed as each new time is input into a cell 6 When you are finished entering time select the Save button former Edit button in the top right corner of the table This saves the User time settings for further uses All five User time settings are entered and saved the same way 30 MYECL Imager User Manual Thermo Fisher Scientific 7 Touch the User button to automatically capture the images 8 Captured images are displayed on the touchscreen and stored in the Gallery for further viewing and selection 31 MYECL Imager User Manual Thermo Fisher Scientific Image Tools Note The Image Tools only function while viewing or printing the image Display Satura
27. ndix A Technical Specifications 52 Appendix B AccesSOFiES ii 53 Appendix C Related Thermo Scientific Products sa amp ep AE eee 55 1 Thank You and Welcome MYECL Imager User Manual Chapter 1 Overview Dear Researcher We thank you for your investment in our Thermo Scientific MYECL Imager We have worked to design a highly functional yet intuitive imager that will provide you with valuable images and data of high resolution and quality This manual has been written as a concise guide for the start up use and customization of your MYECL Imager unit and acquisition software Detailed sections covering the imager image acquisition file management maintenance and troubleshooting provide comprehensive information We encourage you to read the entire manual for a full understanding of the imager but a Quick Start guide is provided if you want to begin using your new imager immediately Thermo Scientific products are always at the forefront of scientific instrument design and we strive to design and produce instruments that can help you achieve your research goals Your feedback is a valuable tool that we use for future product development so please contact us if you have any questions comments or suggestions IMPORTANT Review and implement guidelines for proper set up before installation Locate the imager away from water solvents corrosive materials strong magnetic fields and vibration sources IMPORTANT The
28. ng and selection MYECL Imager User Manual Thermo Fisher Scientific 37 Stop Image Acquisition Image Tools Display Saturation MYECL Imager User Manual Upon touching the Acquire button a Stop window appears over the keypad Selecting the Stop button will stop the current image acquisition exposure and return the screen to the current session exposure settings Note The Image Tools only function while viewing or printing the image Display Saturation Invert and Auto Contrast adjustments are NOT saved with the image file In UV mode while viewing the live image or after capturing an image the Display Saturation button can be selected to highlight the image pixels that are considered to be saturated A red false color is superimposed on the pixels deemed to be saturated Adjustment to the saturation can be done by adjusting the Aperture and or exposure time for subsequent images Shorter exposure times will reduce the light exposure saturation 1 While viewing the live image display select the Display Saturation button under the time keypad 2 The screen will display the saturated areas of the image with a red false color 3 While viewing the live image display in Camera Settings use the Aperture slider bar to adjust the saturation by opening moving slider to left or closing moving Slider to right the aperture Opening the aperture will increase image brightness and saturation Closing the aperture will decrease image br
29. ng begins Delete allows you to permanently delete the highlighted image Thermo Fisher Scientific 44 Image Information MYECL Imager User Manual Information for each captured image is stored in a separate file image file name xm together with the captured image 1 With the captured image on the screen select the Image Info icon at the bottom right of the screen 2 A box appears with all of the relevant image information for that image Beneath the box is a keyboard for inputting personal data into the open fields Only the fields for File name User Project and Notes can be modified 3 After the capture of each image the imager assigns a default name to the image file This image file name can be altered within the Acquisition Information screen Acquisition Information File name Chemi 10s 20111201 1356 User Project Notes ma Exposure time 10s Mode Visible Acquisition time 156 PM Acquisition date 2011 12 01 Camera temperature 22 Of Sensitivitylresolution 3x3 binning A l File name Image title Imager generated or user defined User Person using the MYECL Imager User defined Project Name of project User defined Notes Relevant notes pertaining to image or experiment User defined Exposure time Chosen exposure time for image Mode Single or multi Acquisition time Time of day image was taken Thermo Fisher Scientific Acquisiti
30. nly available with the live image display Pre set Exposure Time 1 Optional Select the Live Camera button if the live image is not displayed 2 Select one of the pre set exposure time buttons using the touchscreen keypad 3 The live image with the new exposure time will be displayed If needed modify the exposure time using the pre set exposure time buttons 0 1s 0 2s 0 3s 0 4s 0 5s 0 6s 0 7s 08s 0 9s Camera Settings A 4 Select the Acquire button to acquire an image 5 Captured images are displayed on the screen and automatically stored in the Gallery for further viewing and selection 35 MYEGL Imager User Manual Thermo Fisher Scientific 36 Custom Exposure Time 1 Select the Custom exposure button using the touchscreen keypad 2 Enter the desired time in milliseconds ms seconds s or minutes m using the touchscreen keypad numbers The m s box is used to input seconds and or minutes and is active by default box is gray in color For a custom exposure time of lt 1 second select the ms box to input milliseconds The shortest exposure time that can be entered is 10 milliseconds this appears as 10 in the ms box Note The live image will NOT display the new Custom exposure time 1 2 3 4 5 6 7 8 9 Acquire 0 Cancel Camera Settings A 3 Select the Acquire button to acquire an image 4 Captured images are displayed on the screen and automatically stored in the Gallery for further viewi
31. oduct No 62237T 62238T 62239 62240 62241 622427 622437 622447 62255 62258T 622617 622671 EW84857 84858T 84859T 84860T Product Name MYlmageAnalysis Software Orange Filter Green Filter Red Filter Blue Filter Chemiluminescence Exposure Screen White Light Conversion Screen UV Exposure Screen Transilluminator Bulb 365nm Transilluminator Bulb 306nm Touchscreen Stylus and Holder Imaging Reference Target One Year Extended Warranty Power Cord C13 Connector North America Power Cord C13 Connector Continental Europe Power Cord C13 Connector United Kingdom lreland t Replacement part for accessory supplied with the MYECL Imager Package Size 1 CD 1 filter 1 filter 1 filter 1 filter 1 screen 1 screen 1 screen 1 bulb 1 bulb 1 package 1 package 1 cord 1 cord 1 cord Thermo Fisher Scientific Appendix C Related Thermo Scientific Products Product No 34087 34075 34095 46640 32209 32132 24615 88518 88018 88610 26616 26619 26614 26634 26625 26628 26612 54 MYEGCL Imager User Manual Product Name Supersignal West Pico Chemiluminescent Substrate Supersignal West Dura Extended Duration Substrate Supersignal West Femto Maximum Sensitivity Substrate Supersignal Western Blot Enhancer Pierce ECL Western Blotting Substrate Pierce ECL Plus Western Blotting Substrate Imperial Protein Stain 1L PVDF Transfer Membrane 0 45um 26 5cm x 3 75m Nitrocellulose Memb
32. ollowing screen RemoteAccess You have selected to allow remote access by Thermo Scientific Pierce Technical Support Please call 800 874 3723 or international 1 815 968 0747 to speak to a Technical Support Scientist and initiate a remote access session Follow the instructions provided and contact our Technical Support team for questions or to troubleshoot the imager U S contact 800 874 3723 International contact 1 815 968 0747 Note Remote access requires the imager to be connected to a network with internet access Contact your facility s IT department regarding correct set up and use of network connections 19 MYECL Imager User Manual Thermo Fisher Scientific Other OS Settings Reset Default Focus Position Update Software Change Username and Password Return 20 MYECL Imager User Manual This function allows the administrator to access the Windows Control Panel to change system and user configurations as needed Note Only qualified administrators should have access to this area Altering configurations can cause imager errors jo ER Control Panel Ad Control Panel items Adjust your computer s settings View ny Smal Kors P ACU Center wore a KG teacup au Restore D ederta Manage Date and T O Dat st Programs Be Dowie Manager mA Devices and Printers EM Display E Fse Op ed eT Die rg Options intemet Options D Keytood P Mouse is Network and Sh g Certo N Performance sion and E
33. on date Date image was taken Sensitivity Resolution setting Binning used for image acquisition Image Gallery The image gallery allows you to view and open multiple previously captured images at one time Image Viewing 1 Select the Gallery tab at the top of the touchscreen 2 Click on the Browse button to open the gallery Thermo chemi EE uv via TE ouer Go o Select Image Using Browse Button og D 2 Thumbnail 3 Thumbnail will display previously taken images Images are separated into sections of days weeks months and years You can use the two right middle arrow buttons or the vertical scrollbar to move and view between the sections If the current gallery contains images from more than 40 image acquisitions the profram will automatically create and move to a new gallery Other galleries can be accessed using the right Gallery arrow buttons TdT The i ER UM EL iit Yis T Gallery 6 i Ban Jarm Raat Biou io G iy kapa Da a y Bay al gt B AE DT Ma lk I i i Minm LEi fil Ss MEd MR IT N ki Pur N Ith TIEF Wi ME BERE N We EET TE Di brki r wn i inji e in mad ET ser Tr sa i wy Ed ee PY Tina HEER n mins NEE Mes ER sd HE TLE Whe Te el id ad ee re Dr FEE ma n mr oe i EE N m w E Le oie De Er To roi LE Ja Win TEE n Min HAI wed TEEL ar Tim TEE va P Cor N ml m Ml E Ed a N aan ETE i EI ipm ES Fr OE y Mima ini sekel sy DE ET Pe as mma aa met b a dama en a en Ts ba oE
34. on the touchscreen and be saved to the Gallery Note A Stop button appears over the keypad once the Acquire button is touched Image acquisition can be stopped by touching the Stop button Note Up to six recent images will be displayed on the touchscreen 2 MYEGCL Imager User Manual Thermo Fisher Scientific Multiple Image Acquisition Vis ER Gallery 0O z z Multi Exposure Settings X G DB Live Pre set C Tap the button to capture images amera Exposur e 1 2 3 4 5 Total Pre se ti 5s 10s 30s 1m 5m 6 45m Exposure Time 10s 30s 1m 5m 10m 16 40m 30 1m 5m 10m 15m 31 30m 10s 15s 30s im 2m 5m 10m 20m 38m 1m 2m 5m Presets im 5m 10m 15m 30m 61m 10m 15m 30m User defined Tap green button to capture tap edit button to update ri 1h EER 1 2 3 4 5 Total Camera Settings A Invert Auto Contrast Display Saturation m x J o 7 c 1 Select the Chemi icon from the top mode settings tabs 2 Optional Select the Live Camera button if the live image is not displayed 3 The white LED lights and live image display are activated Open the imager door pull out the transilluminator and install the chemiluminescence exposure screen black on the transilluminator glass 4 Insert and center the sample on top of the chemiluminescence exposure screen black using the live image display for reference 5 Once the sample is centered close the imager door 6 While viewing the live image adjus
35. or is superimposed on the pixels deemed to be saturated Adjustment to the saturation can be done by adjusting the Aperture and or exposure time for subsequent images Shorter exposure times will reduce the light exposure saturation 41 MYECL Imager User Manual Thermo Fisher Scientific 42 Invert Image MYECL Imager User Manual While viewing the live image display select the Display Saturation button under the time keypad The screen will display the saturated areas of the image with a red false color Using the Aperture slider bar in Camera Settings adjust the saturation by opening moving slider to left or closing moving slider to right the aperture Opening the aperture will increase image brightness and saturation Closing the aperture will decrease image brightness and saturation lf required the image exposure time may need to be increased or decreased to acquire a properly saturated image Exposure time can be adjusted before image capture by using the number keypad select the Display Saturation button again to turn off the false color imaging Note The Display Saturation button is disabled when the Auto Contrast function is enabled Proceed with the steps for acquiring an image The Invert button is a useful tool for viewing faint gel bands This tool inverts the Standard black background with white highlights to a white background with black highlights ie In the active live image select the Invert button und
36. r proper set up before installation Locate the imager away from water solvents corrosive materials strong magnetic fields and vibration sources IMPORTANT The imager weighs 65lbs 29 5kg Use two people to lift it by grasping the bottom and the handle at the rear of the imager Using two people place the imager onto the benchtop given proper space electrical and environmental requirements Remove the outer and inner packaging materials The imager requires a stable laboratory bench or table capable of supporting 65IDS 29 5kg Provide a minimum clearance of 3 inches 7 62cm at the rear of the imager to allow adequate ventilation for camera cooling and access to the power cord and switch In addition allow sufficient space to open the front facing imager door to place sample within the imager Refer to imager dimensions in Table 2 1 Table 2 1 Imager dimensions Dimensions Inches Centimeters Width 12 25 31 1 Depth 22 55 9 Height 21 53 3 A grounded circuit capable of delivering the appropriate current and voltage is required for installation Electrical requirements can be located on the imager label on the rear panel of the imager and in Table 2 2 The imager electrical system will adjust to the proper voltage for the respective country Table 2 3 Connect the imager power cord to the rear left side panel of the system and plug into a grounded power outlet Thermo Fisher Scientific Table 2 2 Imager electrical ratings Elect
37. rane 0 45um 30cm x 3 5m Western Blotting Filter Paper extra thick 8 5cm x 9cm PageRuler Prestained Protein Ladder 10 1 70kDa 2 x 250uL PageRuler Plus Prestained Protein Ladder 10 250kDa 2 x 250uL PageRuler Unstained Protein Ladder 10 200kDa 2 x 250uL Spectra Multicolor Broad Range Protein Ladder 10 260kDa 2 x 250uL Spectra Multicolor High Range Protein Ladder 40 300kDa 2 x 250uL Spectra Multicolor Low Range Protein Ladder 1 7 40kDa 250uL Pierce Prestained Protein Molecular Weight Marker Thermo Fisher Scientific Glossary Aperture Adjustable lens opening controlling the sensitivity and depth of field Camera CCD video camera used to capture moving and still images Chemiluminescence Chemi mode Captures images of sample emitted light Default Standard or home value for a setting Emission filter Optical lens mounted in the Filter Wheel used for filtering specific wavelengths of light Filter Wheel Automated wheel holding filters in front of camera lens Gallery Visible holding area for captured images Hard Main Power Switch Main power rocker switch located on the rear imager panel Lens Camera lens mounted to the CCD camera Plug and play External computer accessories only requiring connection to the computer for operation Resolution The number of camera pixels used to capture an image Sample screens The clear black and white panels placed over the transilluminator during image acquisition Sensiti
38. re screen White light conversion screen UV exposure screen Imaging reference target Quick start guide USB memory device Stylus Stylus holder Power cord with C13 connector Optional items including additional filters and UV bulbs 865nm may be added to the above list depending on the items ordered with the imager Note Upon receiving your imager please verify you have received all of the items listed above Please contact Thermo Scientific Technical Support for assistance Thermo Fisher Scientific Functionality The MYECL Imager provides UV and white light illumination source for imaging of samples Detailed images with varying resolution 1x1 2x2 3x3 4x4 and 8x8 binning of chemiluminescent Western blots and electrophoresis gels Low noise high dynamic range CCD camera detection Hardware and software optimized for image acquisition One touch image acquisition Multiple image acquisition with pre set and user defined settings Multitasking capability to review captured images while acquiring new images MYECL Imager Each imager arrives loaded with the MYECL Imager Acquisition Software This Acquisition Software manual gives detailed information on using all software functions and modes The acquisition software allows you to acquire images using three different modes Chemiluminescence Chemi mode for image acquisition of self emitting chemiluminescent light Ultraviolet UV mode for image acquisition of UV excit
39. re to produce the desired image 7 Select a Pre set or Custom exposure time 1 Select the Vis tab on the touchscreen Changing to Vis mode automatically turns on the UV lights within the transilluminator and the LEDs 2 Optional Select the Live Camera button and enable the UV button if the live image is not displayed Thermo Fisher Scientific 3 Open the imager door confirm that the UV lights are off and install the white light conversion screen on top of the transilluminator glass 4 Insert and center the sample on top of the white light conversion screen using the live image display for reference 5 Once the sample is centered close the imager door The UV lights will turn on 6 Select the UV light button on the right side of the screen to switch OFF the UV lights This produces only reflected light 7 While viewing the live image display adjust the camera settings for Focus Digital Zoom Resolution Sensitivity and Aperture to produce the desired image if needed 8 Select a Pre set or Custom exposure time Pre set Exposure Time 1 Select one of the Pre set exposure time buttons using the keypad O 1s O is 0 2s 0 3s 0 4s 0 5s 0 6s Custom 0 7s 0 85s 0 9s 2 select the Acquire button to capture an image 3 Captured images are displayed on the screen and automatically stored in the Gallery for further viewing and selection Note A Stop
40. rical Parameter Rating supply Voltage VAC 120 240 Frequency Hz 90 60 Power Rating W 120 Fuse power center T4AL 250V 4A Table 2 3 Imager electrical requirements for major countries Region Voltage Frequency Amps A VAC Hz US and Canada 120 60 1 0 Europe 220 50 0 5 UK 240 50 0 5 Japan 100 50 1 2 China 220 50 0 5 India 230 50 0 5 South Korea 220 60 0 5 Environmental For optimal performance maintain a constant laboratory temperature range of 15 Requirements 25 C 99 77 F with relative humidity between 15 70 To allow adequate camera cooling do not place the imager in direct sunlight Computer Requirements The imager is operated by an integrated computer which is accessed through the touchscreen The MylmageAnalysis Software program is included with the imager purchase To run the MYlmageAnalysis Software properly workstations must meet the minimum performance requirements listed in Table 2 4 Table 2 4 Recommended computer requirements for MYImageAnalysis Software Description Recommended Operating System Windows 7 Vista or XP Processor Pentium 4 2 4GHz Memory RAM 512MB Hard Drive 2GB Screen Resolution 1280 x 1024 Refer to the MylmageAnalysis Software User Manual for more information 6 MYECL Imager User Manual Thermo Fisher Scientific 7 Imager Images with Feature Locations Imager Front View MYECL Imager User Manual oar ee eee a Touchscreen Upper USB Port Soft Po
41. s an image as soon as the time button is touched Exposure Time 10s 1m 10m 15m 15s 2m 30s 5m 30m Multi 1h Custom 2 Captured images are displayed on the screen and automatically stored in the Gallery for further review and selection Note A Stop button appears over the keypad area once an exposure time button is selected Image acquisition can be stopped by touching the Stop button Note Up to six recent images will be displayed on the touchscreen Thermo Fisher Scientific Custom Exposure Time 1 Select the Custom exposure button using the touchscreen keypad 2 Enter the desired time in milliseconds ms seconds s or minutes m using the touchscreen keypad numbers The m s box is used to input seconds and or minutes and is active by default box is gray in color For a custom exposure time of lt 1 second select the ms box to input milliseconds The shortest exposure time that can be entered is 10 milliseconds this appears as 10 in the ms box mis ms m s ms 1 2 3 1 2 3 daa ai 6 4 5 6 7 ls 9 Me 8 9 Acquire 0 Cancel LA 0 Clear 3 Once a number is selected the lower Cancel button will become a Clear button Use this to remove any entered numbers Note Selecting the Cancel button will return the custom exposure time keypad to the pre set exposure time keypad 4 Touch the Acquire button to capture the image 5 The image will display in a tab
42. s to change this username and password once the imager is installed To change the password select the Change User Name and Password button EL zigiz Administrator Settings Set Image Set Default Other OS Gallery Location Focus Position Settings Change User am Remote Update Software Name and Password The Set Image Gallery Location button allows the administrator to set up a default pathway for saving acquired images The imager has a default image gallery location Computer Local Disk D Gallery The image gallery location can be changed to another gallery folder Gallery01 Gallery04 in the Local Disk D or you can use a network folder 1 A folder menu will allow for selection of the appropriate location 2 select OK after choosing the appropriate storage location Please choose Gallery Folder to save captured images Desktop My Documen ts dd 4 My Computer a My Network Places Note If the Viewing Gallery becomes slow the administrator can change the image saving location to a different folder to start a new gallery Previously acquired images will remain in the old gallery folder The administrator needs to change the image location back to the old folder to access these old images Thermo Fisher Scientific Allow Remote Access Remote access for maintenance and troubleshooting is possible with the imager After selecting the Allow Remote Access button the administrator views the f
43. t the settings for Focus Digital Zoom and Resolution Sensitivity in the Camera Settings menu if needed Note Camera Settings are available only with the live image display 7 Select a Pre set or User defined Multiple Exposure Time Note A Stop button appears over the keypad once the Acquire button is touched Image acquisition can be stopped by touching the Stop button 28 MYECL Imager User Manual Thermo Fisher Scientific 29 Pre set Multiple Exposure MYECL Imager User Manual Settings The five pre set exposure settings allow you to select a time series of exposures Multi Exposure Settings Pre set Tap the button to capture images Exposure 1 Pre set As Pre set 10s Pre set 30s Pre set4 im Pre set5 im 1 Select the Multi button from the touchscreen number keypad 2 10s 30s im 2m 5m 5m im Tm iim 10m 15m am 10m 15m 20m 30m X Total tm 16 40m 31 30m 38m im 2 Select one of the Pre set option buttons with an appropriate time series of exposures 3 The images are automatically acquired 4 Captured images are displayed on the screen and stored in the Gallery for further viewing and selection Thermo Fisher Scientific User defined Multiple Exposure The User defined Multiple Exposure settings allow you to set custom times in Settings situations where the pre set exposure times do not meet your needs User defined s Edit Please clic
44. ter powering up the imager please allow 20 30 minutes for the camera to cool to the optimal temperature 21 MYEGCL Imager User Manual Thermo Fisher Scientific 22 Camera Settings Available in Chemi UV and Vis Modes MYECL Imager User Manual i Gallery O 3 Live Display 2012 August 30 12 73 08 Default Display Aito Sain wert Contrast Focus Icon for performing minimal focusing adjustments that may be needed as varying object depths change Note An Imaging Reference Target is supplied with the imager Use this target when performing camera focusing Digital Zoom Icon for increasing or decreasing the camera zoom and centering the image acquisition area Resolution Sensitivity Icon for adjusting the camera image sensor s degree of response to light the number of camera pixels used to capture an image The Resolution Sensitivity selection allows for choosing among five different sensitivities 1x1 far left setting 2x2 3x3 middle default setting 4x4 and 8x8 far right setting binning A selection of 1x1 binning provides you with minimum sensitivity maximum resolution while a selection of 8x8 binning provides for maximum sensitivity minimum resolution Table 3 1 provides multiple specifications related to the varied binning sizes Thermo Fisher Scientific Table 3 1 Binning specifications for sensitivity settings Binning Size DPI Pixel x Pixel Image size Image size Pixel size kb pixels inc
45. tion Invert and Auto Contrast adjustments are NOT saved with the image file Display Saturation In Chemi mode after capturing an image the Display Saturation button can be selected to highlight image areas that are considered to be saturated A red false color is Superimposed on the areas deemed to be saturated Adjustment to the Saturation can be done by adjusting the exposure time for subsequent images shorter exposure times will reduce the light exposure saturation 1 After capture of an image select the Display Saturation button under the time keypad 2 The screen will display the saturated pixels on the image with a red false color Display Auto Saturation Contrast a Display Saturation Auto Invert Contrast lf the image appears to be over or under saturated the image exposure time may need to be increased or decreased to acquire a properly saturated image exposure time can be adjusted before image capture by using the number keypad 3 The Display Saturation button can be selected again to turn off the false color imaging Note The Display Saturation button is disabled when the Auto Contrast function is enabled 32 MYECL Imager User Manual Thermo Fisher Scientific Invert Image The Invert button is a useful tool for viewing faint gel bands This tool inverts the standard black background with white highlights to a white background with black highlights or vice versa Original Chemi Image In
46. tor UV light is initially on for 5 minutes User input will then be requested for further use of the UV light If you do not select the Keep UV on button after the initial 5 minutes then the UV light will automatically turn off lf the UV light is needed after opening the imager door the white door peg can be pulled out to turn on the UV bulbs Appropriate PPE must be worn if performing this operation The white door peg returns to a normal position once the door is fully closed Extended UV on position Retracted UV off position UV mode uses the same functional Camera Settings as the Chemi mode but adds the camera setting functions for Aperture UV Light and Filter Wheel Position 1 Select the UV tab on the touchscreen 2 Optional Enable the UV button and select the Live Camera button if the live image is not displayed 3 Open the imager door and confirm that the UV light is off The white LED lights are activated once the door is opened Pull out the transilluminator and place the UV exposure screen clear on top of the transilluminator Thermo Fisher Scientific 4 Insert and center the sample on top of the UV exposure screen clear using the live image display for reference Once the sample is centered close the imager door 5 While viewing the live image adjust the necessary settings for Focus Digital Zoom Sensitivity Resolution and Aperture to produce the desired image Note Camera Settings are o
47. verted Chemi Image 1 In the active live image or acquired image select the Invert button under the time keypad 2 The inverted image can be used for acquiring images or switched back to the Standard image by again selecting the Invert button Auto Contrast Selecting the Auto Contrast button increases the contrast between the signal and the background to increase visibility Image Information Information for each image is stored in a separate file together with the captured image 1 With the needed image on the screen select the Image Info icon at the bottom right of the screen 2 A box appears with all of the relevant image information for that image Beneath the box is a keyboard for personal data input Only the fields for File name User Project and Notes can be modified 3 Upon each image capture the imager assigns a default name to the image file This image file name can be altered within the Image info screen 33 MYECL Imager User Manual Thermo Fisher Scientific Ultraviolet UV Mode 34 2 AA Camera Settings Capture Settings Image Acquisition MYECL Imager User Manual Ultraviolet UV mode is used for the capture of images using transmitted UV light CAUTION UV mode uses the UV lights in the transilluminator Long term exposure to UV light can be harmful Use the appropriate eye and skin personal protective equipment PPE if there is any chance of exposure to the UV light Note The transillumina
48. vity CCD camera s degree of response to light Soft Power Switch Blue lit round button located on the front imager panel Stylus Pen shaped device used to input commands on the touchscreen 55 MYEGCL Imager User Manual Transilluminator Sliding sample work platform containing the UV bulbs Ultraviolet UV mode Captures images using transmitted ultraviolet light Visible Vis mode Captures images using reflected visible light or transmitted light Thermo Fisher Scientific thermoscientific com pierce 2012 Thermo Fisher Scientific Inc All rights reserved Windows Windows 7 Windows Vista Windows XP Excel PowerPoint and Word are registered trademarks of Microsoft Corporation SYBR and SYPRO are registered trademarks of Life Technologies Corporation Deep Purple is a trademark of GE Healthcare Limited UK Pentium is a registered trademark of Intel Corporation All other trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Life Science Research Belgium and Europe The Netherlands the Middle East and Africa Tel 076 50 31 880 Distributors Germany Tel 32 53 85 71 84 Tel 0228 9125650 France United Kingdom Tel 0 800 50 82 15 Tel 0800 252 185 2162390 0 Switzerland Tel 0800 56 31 40 Email perbio euromarketing thermofisher
49. wer Switch i Lower USB Ports Sample Chamber Door MYECL Imager Figure 2 1 Imager front and side views detailing main components Thermo Fisher Scientific 8 Imager Front View with Sample Door Open White Door Peg Epi white Light Bars Magnetic Door Latch Glass Sample Platform Transilluminator Figure 2 2 Imager view with the sample door open and the transilluminator extended MYECL Imager User Manual Thermo Fisher Scientific Imager Rear View Thermoelectric m Camera Cooler Lifting Handle Computer Hard Power Cooling Switch and Fan Power Cord Connector Ethernet Network Connection Figure 2 3 Rear imager view 9 MYECL Imager User Manual Thermo Fisher Scientific 10 Start Up Hard Start Up MYECL Imager User Manual IMPORTANT Allow the imager to equilibrate for 20 30 minutes after each initial hard power up This ensures that the camera is optimally cooled before image acquisition Equilibration is not necessary if you have performed a soft shut down of the imager To hard start the imager 1 Turn on the hard power switch located at the bottom of the rear imager panel The hard power switch provides power to all of the imager components 2 Turn on the soft power switch on the front of the imager This switch activates the internal computer touchscreen and thermoelectric camera cooler 3 The computer boots up and the touchcscreen displays an initial message wit
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