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1. DemoBlue TIF Ta 4 x 0 Y 0 80 90 50 Set Color Best Fit Reset Background none y New You can also combine individual channels from a single image Each channel will be listed separately as shown here Source Images The Source Images combo box displays the images available for color mixing The Source Image list is initially filled with a list of the gray scale images that are currently open The size of the color composite is determined by the first input image selected Once this is entered the list is filtered to contain only images of the same size as the initial selection 126 Help Manual Add Click the Add button to add an image to the mix You will be prompted for the color to be assigned to that image from the Set Color dialog Delete Use this button to delete the selected image from the list Colors The Colors combobox displays the images selected for color mixing Color Composite 3 xj Source Images Adjust l All Selected Delete M Registration j Contrast m DemoRed TIF 4 Spe m X 0 Y 0 To change the color within the list box double click to select the image name the Select Color dialog box appears or highlight the name and click the Set Color button To delete colors images from the Colors list box first click on the color image to highlight it then press the Delete button within the
2. Help Manual UHCCD01400KPA 2298 2298 2395 8 1 100 0 x ce AA RGB BGR In this dialog video Resolution can be selected Combobox if it has The Video Source Property can be set by clicking the Settingeee button Click the Snap button to capture an image This will create a new window and its title bar will be assigned a digital as the image window name Check the RGB BGR box to ensure the correct color encoding format compatible with your application Click X on P to close the Twain Acquire dialog 6 7 Save Ctri S Choose the File gt Save 4 command to immediately store the current window image to its file the file name is listed on the window s title bar while leaving the image still active in its window If the image is untitled or titled with a digit ToupView will issue the File gt Save Aseee dialog automatically The default Save as type will be Window Bitmap bmp dib rle If there are Measurement Objects overlaid on the image the default Save as type will be ToupView File Type tft The File gt Save command can be used to save the most recent changes to disk It is 19 Help Manual often performed as a precautionary measure during lengthy or involved processes to reduce the amount of reprocessing that might be required in the event of a system failure or operational error When an image is closed and not to save its changes is chosen ToupView discards all
3. Double 9 Pixels Rulers and Grids Cursor Cursor Cross v Point Capture Null Misc Select the Horizontal cross in None window default Single single line Double 1 Pixel Double 3 Pixels Double 5 Pixels Double 7 Pixels and Double 9 Pixels formats Select the Vertical cursor in None window default Single single line Double 1 Pixel Double 3 Pixels Double 5 Pixels Double 7 Pixels and Double 9 Pixels formats Single means single line Double means two parallel lines 1 Pixel means the line space between the two lines is 1 pixel in distance The other sizes also have this meaning Select the cursor shape from Cross Point and Null In the figure below a cursor with Double horizontal and vertical lines with 9 Pixels cross for the alignment application the longer one and the Cursor as a Cross was defined Cross double lines with 9 pixels in both directions Cursor Cross 15 1 6 Capture Page If one wish to capture an image with many Measurement objects overlaid on the video window the following settings should be defined first 160 Help Manual 1 Choosing the Options gt Preferencesoos command a dialog called Preferences will be brought up as shown below Preferences Ea Y Capture with Marker and Watermark Quick Save File Capture with Measurement Print Rulers and Grids Cursor Capture Misc Cancel A
4. Note The Folders Sidebar and the Browse can be used to perform tasks such as creating new folders renaming moving and deleting files Individual file information and import data from digital cameras can also be displayed Double clicking the left mouse button on the icon will open the image as an active image in full size See Image window UI in Sec 3 for more details 8 2 Measurement Sheet When choosing the View gt Measurement Sheet command the Measurement Sheet will be active The Measurement Sheet shows the Object s possible features such as Name Center Radius Area Perimeter Angle Start Point and End Point overlaid on the extra layer ToupView BRACKET TIF File Edit View Setup Capture Image Process Layer Measurements Plugin Options Window Help Alo Br micron um Y 40X 100 y Om Ir 774 Malek katie aa Measurement Sheet 2 121283 14 56114 17 3 c3 28829 06 56853 78 4 c4 167210 57 72118 97 v Auto Hightlight Settings Zoom 100 E Measurement Sheet CAIPWIN6O Images BRACKET TIF 760 x 570 T micron x10000 BPP 8 Resolution 3937 3937 ls Clicking the right mouse button on the Measurement Sheeteee and the right mouse context menu will bring up on the Measurement Sheeteee window as shown above They are explained below 38 Help Manual 8 2 1 File
5. After the Video capture is started The 5 button on the Camera Record Record Sidebar will become x Clicking TEE will stop the capture process otherwise it will stop untill the Time Limit is reached After the O Record Video capture process is finished The on the Camera Sidebar will become ee for the future Record process 6 You can choose the File gt Open Videoees command to display the captured video file in the video window 7 Clicking on the video window with you right mouse button will bring up a context menu It includes the View Propertieseee menu to check the Video Properties the Start Pause menu to start or pause the video and the Ful Screen menu to display the video in full screen mode These commands can also be performed by choosing the Setup gt Start Pause Setup gt View Properties or Setup gt Full Screen menu EM Properties 41 Start Pause Pause Full Screen Esc 10 4 Start Broadcasteee Start a network broadcasting service Select the port and the video format before start the service After that ToupView will start broadcasting the video that is opened 64 Help Manual by ToupView locally via the specified port and format The other users could share the video by open broadcasting function Broadcast Parameters Ez Port Save the content to file simultaneously Cies Coma Clicking the Next gt button to set the video Encoder parameters for broadcasting
6. Clicking on an item in the Color Palette will set the Color Value slider to the correct value and update the displays in both the Selected color block and Selected Color text field For example clicking on the white button on the Color Palette will set the Color Value Slider to the right change the Selected color block to white and cause the Selected Color text box to display White Adjust The controls in this section are used to adjust the color composite contribution from each image or for the color composite preview itself You can adjust the input image that is currently selected in the Colors list by clicking the selected button When you want to adjust the color composite preview or you want no image is selected in the Colors list use the Al button 129 Help Manual Registration Registration allows you to correct for mis registrations of images caused by filter induced optical shifts Use the directional keys to shift the selected image in the x and or y direction with respect to the rest of the input images One click moves that channel one pixel in the direction indicated Blank areas are filled with black The key labeled 0 0 will re center the selected image The registration shift for the selected channel or the overall image is displayed in the static text box below the directional keys Contrast Use the BCG sliders along with the Best Fit and Reset buttons to adjust the contrast of the selected
7. Description Weighted Average method produces a soft fusion result but may miss detail This method often gives superior results to FFDSSD method with deep stacks such as images produced by microscopy It is worth noting that images to be processed must be in Z order closest to furthest or furthest to closest with the microscope objective lens relative to sample on the stage Maximum Contrast method is similar with weighted average method and the input images must be in Z order as well The difference is that the maximum contrast method may loose some detail though what detail is preserved will be sharper Maximum contrast method is a preferred method of EDF FFDSSD is a method that does filtering in the frequency domain using Fast Fourier transform FFT and does synthesis in the space domain FFDSSD can be used for images having misalignment Users can manually or automatically select reference images to make input images aligned lt Back Cancel There are 3 EDF methods in ToupView they are Maximum Contrast Maximum Contrast method is similar with weighted average method and the input images must be in Z order as well The difference is that the maximum contrast method may loose some detail however the preserved details will be sharper Maximum Contrast method is a preferred method in these 3 Help Manual Weighted Average Weighted Average method produces a soft fusion result but may lose detail This method often g
8. File Prefix Cursor File type png M Option Capture Sample 0001 png Misc Y Show the rename dialog The above settings will be applied to Quick Save menu and the corresponding button in the toolbar When calling Quick Save images will be saved quickly according to the above settings in the specified directory Cancel Apply Directory Enter the name of the drive and directory where the new image will be saved You may either type the path information or use the Browse button to locate it from a standard Browse Folder dialog Name Format The year month date hour minute and second or nnnn sequence are used to as part of the file name If more files are saved with in a second a xx suffix is attached to the end of Name Format to avoid the possible name confliction For the nnnn sequence Name Format no suffix is needed File Prefix Enter a file name prefix for Quick Save when generating files names for a series of images This prefix will be combined with Name Format to form a final file name naming paradigm 155 Help Manual File Type In this combobox select the format in which you want the image to be saved can be BMP JPG PNG TIF Click Option button to set the different parameters for encoding the file For BMP format the Option will be disabled See File gt Save Aseee menu about the details of the format encoding methods If there are Measurement Objects over the image the File Type
9. Top hat O Well Options O 2 x 2 Square 3 x3 Cross O 11 x 11 Circle O 3x1 Row O i E 5 x5 Circle Passes 2 Sl O 1x3 Colomn 7x7 Circle Undo OK Cancel Apply 3 Choose Process gt Segmentation amp Count gt Color Cube a Color dialog will pop up as shown below 4 2 3 gt ema RGB 180 37 62 OK Options Tolerance 4 At this moment the Colorizing Pipette is checked Click the pixel wish to segment that is to say select the color area which is interested in If some undesirable color area is included in the segmentation area just check the De Colorizing Pipette and click the undesirable pixels thus the undesirable pixels will be wiped off When the segmentation process is completed click OK and the Segmentation amp Count operation will be carried out 118 Help Manual The result of segmentation after the first The result of segmentation after the clicking at the pointed area with second clicking at the pointed area with Colorizing Pipette Colorizing Pipette The result of segmentation after the The result of segmentation after countless second with the De Colorizing Pipette clickings with Colorizing Pipette slight difference exists as inaccuracy in position 5 Click Options to set Outline Label and etc the final results is shown below So far the primary segmentation is completed 119 Help Manual 12 4 7 Split Objects It can be found ou
10. if it is not in the current folder or selecting Files of type to obtain a list of file names Double clicking a file name in the large combobox where both folder and file names are listed will automatically open it Note If just type in the file name be sure that the Files of type field correctly identify the format of the file to open Otherwise error messages will bring up when ToupView tries to open the file Files of type In this list box select the image format of the file to open If one selects All supported formats ToupView uses the file s extension to identify its format ToupView supports the following file formats Window Bitmap bmp dib rle JPEG jpg jpeg jpe jif jfif Portable Network Graphics png Tag Image File Format tif tiff Compuserve GIF gif Targa tga PhotoShop psd ICON ico Enhanced Window Metafile emf Window Metafile wmf JBIG jbg Wireless Bitmap wbmp ToupView File Type tft If the image file does not use standard format identifying extensions the file in the File name field must be typed and then select its format from the Files of type list box Otherwise ToupView will select a format Help Manual o based on the file name extension Preview Click this button to preview image in small size In preview mode statistics and information about the image i e image Width Height and image location will be displayed The default is no Pre
11. if the camera is started this menu will be disabled and the video file cannot be opened anymore 6 3 Open Broadcasteee Users could receive the broadcasting video by ToupView from the other user by the IP and port For example if a user starts a broadcasting service via IP 192 168 0 20 and port 2080 the other users could share the video that is opened on 192 168 0 20 by inputting the right IP and port The performance will depend on the bandwidth of the users If the receiver and the broadcaster are in one intranet the performance will be excellent If not the performance will depend on the bandwidth Open broadcast ES Address Port 2080 6 4 Camera List The File gt Camera List submenu will be disabled when there is a video file opened in the ToupView frame or a camera start up ToupView will detect all of the cameras that your computer has installed Here it is UCMOSO3100KPA a 3 1M pixel CMOS camera and will append all the camera names as submenu to the File gt Camera List menu Here the submenu name is UCMOS03100KPA Choosing the File gt Camera List gt UCMOSO3100KPA will create a video window and start the video stream The video window will be associated with the name of Video UCMOS03100KPA i e its title bar name will be Video UCMOSO3100KPA There are 3 methods to start the video stream they are File gt Camera Choose the File gt Camera List gt UCMOSO3100KPA Here a 3 1M Li
12. properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 12 Two Circles P 14 12 1 Two Circle gt Center Radius Draw two circles with Center Radius method on the specified layer After the two circles are drawn a line connecting these two circle centers will be drawn Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 12 2 Two Circle gt Three Points Draw two circles with Three Points method on the specified layer After the two circles are drawn a line connecting these two circle centers will be drawn Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edi
13. see the Edit gt Copy menu If there is no image data on the clipboard the File gt Paste as New File menu will be disabled Choose the File gt Paste as New File command to place the contents of the clipboard image into a new image window which becomes the active image The new image type will be the same as that of the original image ToupView will accept image data from other applications via the clipboard as long as it is in Windows Bitmap DIB format Note ToupView will assign a digit to the Paste as New File command created image window title bar 6 12 Print Setupeee Choose the File gt Print Setupeee this command to access the setup panel for the printer that has selected ToupView will present the standard setup panel for the particular printer this is the same panel one would receive if one were setting up the printer from the Windows Contro Panel Change printer s setup to satisfy the requirements click OK button to return 6 13 Print Previeweee Ctri Shift P Choose the Print Preview command to see the real time effect of the printer without actually printing it out 6 14 Printeee Ctrl P Choose the File gt Printee command to print one or more copies of the current image to the selected output device The ToupView File gt Printeee command lets one take full advantage of the printer s capabilities If the printer has built in half toning or color dithering capabilities use them or instruct ToupView to perform these
14. segmentation corresponding to Right Vertical Line position in histogram Users can segment the pixels bigger than the Left Value and smaller than Right Value by inputting a value in this edit box or dragging the Right Vertical Line in histogram When input a value in this edit box users should click enter to make the value effective Meanwhile the Right Vertical Line will move to the place the Right Value represents Right Value range current Left Value 255 The current Right Value is 79 A ere channel of the eae RGB histogram It can be R Z show AR Show all channel RGB Dee an When uncheck Show All only a AAA Mo y Used to identify the Color of the nie pixels The default is Doo pa a e Options When users click Options button a Count Options dialog pops up as below The setting of Count Options dialog is the same as that of Waltershed s Count Options dialog details can be referred to Process gt Segmentation Count gt Watershed W 108 Help Manual Count Options Approximation None With holes Outline Style Outline Color m Default M Label Type Y Area px Minimum Maximum Y Perimeter px Minimum Maximum Example of RGB Histogram is as follows 1 0pen an image and then choose Process gt Segmentation amp Counting gt RGB Histogram menu a Histogram dialog shows as below When the mouse moves in the image area the cursor will be
15. to the clipboard Then the Edit gt Paste menu will be enabled One can then Paste the objects onto the Current Layer or onto the other Measurement Layer in the same video image window If one switches to the Background Layer the Edit gt Paste menu will be disabled but if one returns to the Measurement Layer the Edit gt Paste menu will be enabled again Note a The Edit gt Copy command will not delete the Measurement Objects over the image Any data existing on the clipboard will be replaced with the new data b The copied Object s can be pasted into the active window or into another opened window using the Edit gt Paste command as long as the current window is not on the Background Layer the Edit gt Paste menu will be disabled if the Background Layer is active See the View gt Sidebar gt Layer command in Sec 8 and the Layer menu in Sec 13 for details 7 6 Paste Ctrl V If there is Measurement Object data in the clipboard and the Current Layer is not the Background Layer the Edit gt Paste menu will be enabled Choose the Edit gt Paste command to Paste Objects from the clipboard onto the active image s Measurement Layer One can also choose Edit gt Paste command to transfer a layer s Measurement Objects from one image window s Measurement Layer to another image window s Measurement Layer Note The Edit gt Paste command does not support the image area Paste operation 7 7 Image Select a The Edit gt Image Select command can be used to
16. 3 Move the mouse to point 3 mark the final point by clicking the left mouse button A label A1 50 26deg will be shown near point 2 Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the 142 Help Manual selected Object type The type could be the same or different 14 3 Point E P1 36331 ES 12638 41 Jurn i 4K a Pe Ts A Sm Se ae da Move mouse to the point click the left mouse to mark it It will show the point Label Pn and its position x and y over the image Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or d
17. Color Composite dialog box Color Bar The picture item box on the left side of the Colors list indicates the color associated with each image in the composite The Color Bar will scroll with the contents of the Colors list box Set Color This option determines the Color value used for the selected channel in the final image display Clicking on the Set Color button brings up the Select Color dialog box 127 Help Manual x For File DemoGrn TIF EE O E J1 Slected E Red 000 Green 255 Blue 000 Current Red 000 Green 255 Blue 000 A Lao e Directly select a color by 1 Dragging the Color Value Slider to the desired color on the Color Bar range of O to 359 or 2 Clicking the appropriate Color button on the Color Palette The Color Bar represents Color degrees in the standard color wheel representation and the Color Palette contains a subset of possible Colors pure red green blue cyan magenta and white or 3 Select a dye in the dye list and click the Select Dye button Note You can navigate through the Select Color dialog box by using the lt Tab gt and lt Shift Tab gt keys to cycle through the items in the dialog box Intensity is determined by the image the Color is applied to and saturation will always be 100 The only exception to this is the white Color which has by definition a saturation of 0 Selecting a value greater than or equal to 360 draggi
18. Frame 8293 Actual Frame Rate 13 2 Grab Frame Count 8294 Grab Count 8295 Grab Retry Count 8390 Grab Frame Speed 13 2 Grab Percent 100 0 OOOO OO Magnification j L aa Clear All ee Up Down A ToupView H Measurement Sidebar I Measurement Sheet K Auto Hide button L Horizontal ruler M Vertical ruler N Frame Rate O Frames captured i Sa ayer Measurements Plugin Options do Cil B micron um y 10X y W 03 a P 5 42 1 D0000 0 3M EG B Menu C ToupView toolbar D Camera Sidebar E Folders Sidebar F Undo Redo Sidebar G Layer Sidebar J Statusbar P Current Video sizes Folders Undo Redo Layer y Measurement lolx nanometer nm micron um millimeter mm centimeter cm meter m inch in Customize Angle Unit Help Manual Q Selected microscope Magnification R Current Unit AA Sidebar right mouse button context menu AB Video window right mouse button context menu AC Browse window right mouse button context menu AD Image window right mouse button context menu AE Frame window right mouse button context menu AF Double click bring up Video Properties dialog AG Double click bring up Magnification dialog AH Double click bring up Measurement dialog AI Horizontal Ruler or Vertical Ruler right mouse button context menu 2 2 How to close the video window bx 1 Double clicking the tabbed video window title or clicking x o
19. Manual The Auto Grids can be set in the Ruler and Grids gt Settingeee menu 8 7 4 Grids gt Remove All Grids Remove Delete all of the Manual Grids or Auto Grids overlaid on the image video window 8 7 5 Settingseee Choosing the View gt Ruler and Grid gt Settingeee command or choosing the Preference gt eee command and clicking the Rulers and Grids page realize the same function Choosing the Preference gt eee command and clicking the Rulers and Grids page can set Ruler Color Cursor Color of the Horizontal Ruler and Vertical Ruler for the video or image windows The Grid Style Line Style and Line Color for the Grid overlaid on the video and image window can also be set Preferences Quick Save Ruler Color Default File Cursor Color E Default bd Print Grid Rulers and Grids Style Manual Grid y Cursor Line Style Solid Capture Line Color Misc Ruler Color The Horizontal Ruler color and Vertical Ruler color Default is blue 0 0 255 Cursor Color The Cursor color When you are moving your mouse over the video image window there will be Horizontal Cursor and Vertical Cursor move on the Horizontal Ruler and Vertical Ruler moving at the same time indicating the current Cursor position in both directions Default is Red 255 0 0 Grid Style The Grid Style can be No Grid Auto Grid or Manual Grid Default is No Grid 47 Help Manual Grid Line Style The Line Style for the grid c
20. Marker type can be Cross Rectangle Circle Cross Rectangle or Cross Circle The Video Marker is shown as below Video Overlay Overlay Cross Cirde Choosing Cross Retangle in the Type combobox and Video Overlay Marker dialog will become 52 Help Manual Video Overlay xj Overlay e A a Cross Width Cross Height 100 100 Rectangle Width Rectangle Height 50 50 x Offset y Offset 0 0 Rotate p Edit the Cross Width and Cross Height Rectangle Width and Rectangle Height x Offset and y Offset in their specific fields Click Color to define the Video Marker color Click OK to end the Video Marker dialog and a Cross Rectangle Marker will be overlaid on the video window as shown below 2 TF eq fs i jw a ts ae ha 9 i gies sets a Click Cancel to cancel the Video gt Overlay Marker setup and return to the application area or Apply to overlay the Marker on the video and keep the Video Overlay dialog there for further modification 9 5 Video Watermarkeee Fig 1 shows a micro ruler The dark lines can be extracted as Video Watermark and overlaid on the video window The steps are 53 Help Manual ss 1 Choose the Capture gt Capture Image command or click la gt to capture the micro ruler image as shown in Fig 1 2 Choose the Process gt Binary ee command to binarize the image as shown in Fig 2 3 Choose the Image gt Adjus
21. The Thumbnail mode displays small preview images you can select different view modes in the Browse window Choosing the Icons gt Small Icons will display the image files in Small Icon mode in the Browse window 8 5 Refresh FS This command is for the Browse window only If the files under the Folders Sidebar s directory are altered outside of ToupView after switch back to ToupView one can Refresh the image files in the current directory to update the Thumbnails with View gt Refresh command 8 6 Sidebar There are 5 Sidebar in the ToupView frame window in tabbed format They are Camera Sidebar Folders Sidebar Undo Redo Sidebar Layer Sidebar and Measurement Sidebar 8 6 1 Sidebar overview BA BB BC BD BE BF CA B CC CD CE CF o s a X HE Desktop S s index aton i n es Open t 2 Curve Adjustment a Cu o o O NREN o t Ha ca DAT p aj AA Camera Sidebar AB Camera Sidebar groups for the control of the started up camera BA Folders Sidebar BB Back to the previous folder BC Forward to the next folder BD Browsing the pictures under the directory of ToupView s directory BE Auto hide button to show hide the current selected directory this will open the Browse window if it is not opened Double clicking on the selected directory will perform the same functions BF Folders to locate the Browse window s file directory 42 Help Manual CA Undo Redo Sidebar CB Operation C
22. Windows gt Windowseee will open a dialog box that lets you manage the currently opened windows 174 Help Manual Windows Select window Activate 0001 E 0002 OK E 0003 El 0004 Save venal Close Window s i 0007 i 0008 J0009 1 Video Integrated Webcam The dialog allows you to manage large lists of open windows by assigning them into groups Let us say you have eight windows open but want to close four of them scattered through the list 1 Choose the Windows gt Windowseee command 2 Select the windows you want to close Hold Ctrl or Shift key to select more than one at a time Here 4 windows are selected Windows Activate Selectwindow 0001 0002 oK Focos Save A 0006 Close Window s i 0007 F 0008 0009 1 Video Integrated Webcam 3 Click Close Window s button those four selected windows will be closed leaving the other files or captures available for editing You can use this command to switch to the checked window on a list by hitting Activate or bring up from an icon multiple windows 175 Help Manual 17 Help 17 1 Help Contents Choose the Help gt Help Contents command to load ToupView help file The help file is in Portable Document Format PDF PDF is a file format used to represent documents in a manner independent of application software hardware and operating systems Each PDF file encapsulates a complete description of a
23. aie RRL aua Ei ii ES AG SRL RG GSR RU ESR RAE GEE aa RRL Bie RR ce Ara Ree AES 27 Die hk eee eee eee E ee eee eee ee ee eee ee ae ee ee ae ae ee ee 28 1 Undo Redo CELIA ara ar iaa ER RARER EERE GEES 28 2 PORWELC iio O O towne REA A A AA A ARA ee a a 29 i DAC KWIT as E E A O E E EE Se 30 Ted Cut CUBA as ANO OD aaa eee ee eee ee 31 7 5 Copy a AAC EEE ERITREA 31 1 6 Paste Crt ans arsura araara araara areara re se areara aro ee arenai 33 7 7 Image Select Mii ee E E E 33 7 8 Select All CELIA Ninot csee asina iver ewer AA AA AA a 34 7 8 1 Select all on the background layer oooooooooooooooooo o 34 7 8 2 Select all objects over the background layer 34 7 8 3 Select all files in the browse Window ee aaa 35 7 9 Select None COPIA aero 35 TLO Inverse Selection nene REESE 35 7 11 Delete File Delete iaa AAA A A A AA A 35 VIe A A E E EEE E E EE E E E h 37 8 1 Browse Oe AN 37 ds LL Open the browse WINAOW gt desicion 37 8 1 2 Browse window right mouse button context menu o o 37 8 2 Measurement Sheet rocio 38 Bridal Fil IMPOrC Se cis ad eee ei 39 Contents 8 242 ES A pa ckee gear a Game SG Ge eG ee are a eae Ghee a a a a ag 39 Geen qe creo LO Hemi A AS bees bree bere bee bee eee 39 8 2 4 Export to PXcSl sicario AAA ze 39 E AUCO Ardn LION eee RR ANN 39 ia AA II III 39 Bro POLE oia aaa daa 40 Aula e AR A A ee eee eee 40 AA Sort gt Sort by EE 40 ce ias oso e RPP TETERA ATAR 40 A rs as NN 41 A aca enn
24. changes made since the last File gt Save operation Note a The File gt Save command always saves the contents of the entire window even if there is an AOI Area of Interest defined on it b The File gt Save command will be disabled if the file is not changed or the changes have been saved 6 8 Save ASeee Choose the File gt Save Aseee command to store the contents of the current window to a specified file format At the end of a File gt Save Aseee operation the image window will be associated with the new file and the new format i e its title bar will display the new file name ToupView supported file save formats are Window Bitmap bmp dib rle JPEG jpg jpeg jpe jif jfif Portable Network Graphics png Tag Image File Format tif tiff Compuserve GIF gif PCX pcx Targa tga JBIG jbg ToupView File Type tft Save in Find the folder where the file wishes to be saved A new folder may be created using the New Folders button File name Enter to be saved file name To specify the file s location either enter its entire path disk and folder or specify its location using the Save in list box 20 Fe My Recent 6th2002exlarge jpg a 2004 hm 24 large jpg E 2008 hm 20 large jpg E 2010 SA e 0s 2010 large jpg E 2005 hm 14 large jpg 1 2008 hm 33 large jpg 2010 e 10th2001exlarge jpg E 2005 hm 18 large jpg E 2008 hm 62 large jpg 2010 E 10th2004exlarge jpg E 2005
25. color to extract For every pixel if selecting Red color to extract only information about the red channel will be kept and Green and Blue information will be discarded Select Color xi Color E Red acil Cancel Green y Blue E See Filter Coloreee for another color operation 11 2 11 Invert Choose the Image gt Adjustment gt Invert command to reverse the pixel values of the active image without going through the lookup table 11 3 Rotate Choose the Image gt Rotate command to rotate the entire image One has the following submenus 11 3 1 90 CW Rotate the image clockwise by a quarter turn 11 3 2 180 CW Rotate the image clockwise by 180 degrees 11 3 3 270 CW 75 Help Manual Rotate the image clockwise by 270 degrees 11 3 4 Arbitraryeee Rotate the image by a specified angle If choosing this option enter an angle between O and 360 degrees in the angle text box and check CW or CCW to rotate clockwise or counterclockwise For the Arbitraryeee operation it will invoke an Image Rotate dialog like below xi ta eras Degree cw Epa E C ccw Cancel Quality Bilinear Degree The degree that the image to be rotated CW Rotates the image clockwise CCW Rotates the image counterclockwise Quality One can select one of the three methods for the image rotation among Nearest Neighbor Bilinear and Bicubic The default is Bilinear 11 3 5 Flip H
26. command to illustrate how pixels along a selected line are distributed by graphing the number of pixels at each color intensity level Choose the Measurements gt Arbitrary Line or Measurements gt Horizontal Line or Measurements gt Vertical Line command to draw a line on the image and choosing the Process gt Line Profile will bring up a Line Profile dialog as below The procedure can be 134 Help Manual inversed Title Capture I i n Copy Position Save as Aly 2h Ahad Manadh Ma 156 195 204 Line Profile In a Line Profile the X axis represents the spatial scale and the Y axis represents the intensity values which range from O to 255 If the image is a gray scale image the gray value will be profiled If the image is a HSV color image the R G and B values will be profiled separately with the corresponding R G and B colors Background Open the windows color dialog to set the background color of the profile window Title Use this command to set a title on the Line Profile image Capture Capture the image in the Line Profile window as a new untitled image Copy Copy the Line Profile window s content onto the clipboard Save aseee Save the Line Profile image in bmp format 12 12 Mosaiceee Mosaic is a process that can combine the images opened into a new image This will open the following dialog 135 Help Manual Mosaic Images x ImgList Propert
27. displayed as Color Picker Cursor Histogram Cancel OK Options M5 0 79 amp Green Me v Show All RGB 249 51 31 2 Move Color Picker Cursor to any pixel of the image shown as above RGB value of the current pixel will be displayed in the bottom Histogram window Here R is 249 G is 51 Bis 31 showing the RGB value of the ideal segmentation area record the three values Move the mouse to the background area the RGB value of Color Picker Cursor is 79 79 79 In order to accurately segment out the target users can set RGB values of the segmented pixels in the initial interval and those of background out of the initial interval For example the RGB values can be set to the initial interval as follows R is set to the initial interval 200 255 109 Help Manual G is set to the initial interval 0 60 B is set to the initial interval O 60 When setting completed the segmentation effect is shown as below 4 Move again the Color Picker Cursor to any other unshadded pixels then the RGB value shown in left bottom of histogram is 237 70 45 as below Histogram Cancel a Options o 50 100 150 200 o E fa E Green Y Y Show All RGB 237 70 45 It s easily found that here G channel defined 60 is too small to get the pixel segmented Then initial interval for G channel can be adjusted from 0 60 to 0 80 to segment the pixels nea
28. ee ee ee ee eee 151 14 12 1 TWO Circle gt CentertRadi s sa a a aea AA AAA RA AA AA 151 14 12 2 Two Circle gt Three POLOES 2 mes dea a ao ts ia aaa 151 AS AO Ml oso ASS Si 151 IA ay Tese a E E ia OR A A bees beer ieee bees 152 14 15 Polygon AE TIT 153 Tar LOA Order A RN 154 15 ODPELON S e e e e a e 155 l rd Preferences NOD II 155 Lor Ll el Saye Padge o o E A A AO AE AE AE 155 Wieder File P saacana nana na aaka aa A A ea A A A A A A A A a ee 156 ll PINE DO root e E E E E o 157 15 1 4 Rulers and Grids ados scsciaiaRana sas asas aaa asa sa aa 158 deer Cursor Pace onandsosdossdsdsdsssjsosdsdadesododsde sa 159 e A A e ass Page RO 160 Lele MLSO Pagas sisi ola 161 A e sw goea pa i e ie e ae aa ee ee 162 Tos L5 10 Lo hee Ls OO 15 4 1943 LB i 16 Lbs be 16 NO 16 OO Ly Lie FR a NO Contents Za General PTY saw oe 008 He Oe we EMER ER ER ER EMS SESE MERE SE EES 162 See Length Unike PES elen wire ante aia eel me we Oe RO Re ot ee O ele L63 Se Angle UNTIL Dae 45 sas heehee SONS RE OEM ER EWE Nane ennai 164 Zp Sheet PAS 3 5 000000 oe HE RE He ERED EWE SWE WE Oe OK Ow Ba OH OH 165 2o O O a ae bape a E an Koad od a a S AS 166 MaAGnINGarTOns e mirare HS R88 RE OS OE EO BE MER ERE HEHE ROSE Dd MES 166 Calibratesee _ ar ee er A ee ee er ee ee eee ee 167 EATE Dye LISTS espre WS RI we aeae REE EE EMER CSE nse 170 AUTO RODLTESTLONE SS sissa A wm ALAN A LAZ WLIO Weta ue 6d Sak 8 6 0 S
29. gray area and click the White Balance button to establish the video White Balance for future video streaming process 3 If the automatic setting and the actual result still has deviation drag the Temperature and Tint slides to left or right to manually correct the White Balance 5 5 Color Adjustment group 1 Hue Adjusts the Hue of the video Drag the A amp Color Adjustment Hue slider to the right to increase or drag to the left Saturation to decrease hue 2 Saturation Adjusts the Saturation of the Brightness video Drag the slider to the right to increase or drag to the left to decrease video saturation 3 Brightness Adjusts the video Brightness pr Drag the slider to the right to increase or drag to the left to decrease the video brightness Help Manual 4 Contrast Adjusts the video Contrast Drag the slider to the right to increase or drag to the left to decrease the video contrast 5 Gamma Adjusts the video Gamma Drag the slider to the right to increase or drag to the left to decrease the video gamma 6 Defaults Click the Defaults to clear all the changes and reset them to default ones 7 All of your settings will be saved for future Color Adjustment application 5 6 Frame Rate group AS Drag the slider to the right High to ensure Low that the camera can run the video at the fastest Move the slider to the right to ensure that the camera plays live video at t
30. hm 34 large jpg E 2008 hm 65 large jpg E 2010 Desktop 1 12 2008 large jpg E 2005 hm 54 large jpg 2009 4 large jpg E2010 E 14 2008 large jpg 15 2005 hm 68 large jpg 2009 6 large jpg 15 2010 D 14th2006exlarge jpg 2006 7 large jpg 1 2009 hm 1 large jpg 2010 17th2002exlarge jpg 2006 hm S large jpg 1 2009 hm 6 large jpg E 2010 My Documents Help Manual E 18th2002exlarge jpg E 18th2007exlarge jpg E 20th2007exlarge jpg E 2004 8 large jpg 2004 hm 23 large jpg 2006 hm 36 large jpg 2006 hm 37 large jpg 2006 hm 42 large jpg 2007 hm 14 large jpg 2008 hm 16 large jpg 2009 hm 12 large jpg 2009 hm 15 large jpg 2009 hm 36 large jpg 2009 hm 46 large jpg My Computer deals E 2009 hm 50 large jpg Cancel Option 2004 hm 8 large jpg 2007 hm 20 large jpg in T ETT File name 2006 hm 36 large jpg Places Save as type JPEG jpa jpea jpe jif fif Save as type In this list box select the format in which the image wants to be saved Save As is also used to convert a single image from one format to another For example if a TIFF file needs to convert to PCX format open the TIFF image first then choose Save As command with the PCX format option to save it to a new file The default Save as type will be Window Bitmap bmp dib rle If there are Measurement Objects overlaid on the image t
31. in proportion to the hue measured as a percentage from 0 gray to 100 fully saturated On the standard color wheel Saturation increases from the center to the edge Intensity is the relative lightness or darkness of the color usually measured as a percentage from 0 black to 100 white The HLS model is very similar to the HLS color model The main difference between them is the calculation used to produce the brightness value In the HLS model a pixel s brightness L is derived from its three R G and B color values In the HLS model a pixel s brightness L is determined by the minimum and maximum values of its three color values Color Modules RGB CMYK HSI C HLS Preview Check this button to display the real time effect when the slider bar s position is modified The values beside the slider bar show the color changes in various color channels For RGB channel values they can range from 100 to 100 Default is O For CMYK channel values they can range from 100 to 100 Default is 0 For HSI channel values the H value can range from 180 to 180 the S value can 73 Help Manual range from 275 to 275 and the I value can range from 442 to 442 Default is 0 For HLS channel values the H value can range from 180 to 180 the L value can range from 100 to 100 and the S value can range from 100 to 100 Default is 0 11 2 7 HMSeee Choose the Image gt Adjustment gt HMSeee command to ad
32. mark ROI and Copy the selected ROI to the clipboard This command is only used to select the ROI on the Background Layer 33 Help Manual Choosing the Edit gt Image Select command will check this menu or click on the toolbar will keep it down and the cursor will turn into E After the area is selected and the Current Layer is the Background Layer the Edit gt Copy menu will be enabled and then the selected area can be copied to the clipboard for further application To select an area 1 Choose Edit gt Image Select command Or click the Image Select button on the toolbar 2 Drag the mouse cursor across the image with the left button held down until the area is selected 3 Release the button and the area will be marked Handles will appear on the area that will allow altering the selection after it is marked Current Name E M Round Rectangle Note Only when a ROI is selected and the Current item is checked on the Background in the Layer Sidebar the Edit gt Copy command can copy the selected image ROI to the clipboard 7 8 Select All Ctrl A 7 8 1 Select all on the background layer When the video image window is active and the Background Layer is checked choosing the Select All command to select all pixels on the Background layer within the canvas shortcut Ctrl A 7 8 2 Select all objects over the background layer When the video image window is active and the Background Layer is
33. pixel whose value is being modified is usually the ar center most pixel One may however designate any pixel ToupView signals the pixel to be changed by putting a box around it Choose the X and Y Offset spin buttons to apply Click to create a new filter kernel The Edit Kernel dialog will appear The id functions of the dialog are the same way as the dialog for Edite ee described above with the exception that the file name for the new kernel file must be provided Click to delete the selected filter kernel file PP The choices in this group box will vary depending upon the kind of ions selected filter 12 2 Stitch 12 2 1 Video Stitch When the video window is active choosing the Process gt Stitch command will invoke the video Stitch dialog as below Stitch x Direction Horizontal z Run Background v Snap 0 Direction The image stitching direction Horizontal or Vertical The default is Horizontal 89 Help Manual Run The stitching run method Background or Foreground The default is Background The captured images from the camera Click to capture image from camera Reset When an image or images is or are captured the Reset button will be enabled click Reset will remove it or them from the memory It will be disabled when there is no captured image OK Click Ok to begin image stitching This button will be enabled only when the snapped images are more than 2 Click to cancel the stitching pr
34. pixels 352800 count Percentile Histogram ee Chane E H A pixels 352800 level pixels 352800 count count Percentile Percentile If the image is RGB true color choose R G and B to display a composite Histogram of the individual color channels in color Do one of the following To view information about a specific pixel value place the mouse pointer in the Histogram To view information about a range of values click down the left mouse button and drag it in the Histogram to highlight the range The dialog displays the following statistical information below the Histogram Pixels Represents the total number of pixels used to calculate the Histogram 79 Help Manual Level Displays the intensity level of the area underneath the pointer Count Shows the total number of pixels corresponding to the intensity level underneath the pointer Percentile Displays the cumulative number of pixels at or below the level underneath the pointer This value is expressed as a percentage of all of the pixels in the image from 0 at the far left to 100 at the far right 11 7 Resolutioneee Choose the Image gt Resolutioneee command to set the image Resolution to calibrate the spatial scale By default ToupView expresses spatial measurements in terms of pixels This Resolution command is used to change the terms in which ToupView reports such measurements This command
35. principal edges in an image The Sobel applies a mathematical formula to a 3x3 neighborhood to locate and highlight its edges neighborhood is the one that is replaced Sculpt Check this filter to apply a sculpted effect on the image Check this filter to detect and emphasize horizontal edges Check this filter to detect and emphasize vertical edges Options Roberts Check this filter to enhance fine edges in an image The Roberts filter is not a convolution filter It applies a mathematical formula upon a 4 x 4 neighborhood to produce its effect The upper left pixel in the 1 If one of the Edge filters has been checked the options will relate to kernel size and filtering strength The following options will be displayed 3x3 Check 3x3 kernels to produce a more subtle filtering effect 84 Help Manual Check 5x5 kernels to produce a moderate filtering effect Check 7x7 kernels to produce a more extreme filtering effect Passes Enter the number of times that the filter will be applied to the image When a filter is applied multiple times its effect is amplified by each pass An image that has been softened by one pass of the Image Enhancement Filter will be softened further by a second pass Strength Enter a value from 1 10 that reflects how much of the filtering effect to apply to the image A value of 10 specifies that the full strength 100 of the filtered result will be applied to each pixel Values less than
36. proper Tolerance value according to their experience The default R G and B Tolerance values are 10 gt Used to mark the color of the segmentation area The default Color is green Options When clicking the Options button a Count Options dialog as below will pop up This dialog is the same as that in Process gt Segmentation amp Count gt Watershed W Please refer to Process gt Segmentation amp Count gt Watershed W for the detailed operation of this dialog 116 Help Manual Count Options Approximation With holes Outline Style d Color E Default Label Type Area px Minimum Perimeter px Minimum Here is an example to demonstrate the Color Cubic segmentation process 1 Open an image as below 2 Because of the inevitable noise in the image an image smooth operation should be done first There are many algorithms to perform the image smooth operation and here we are to process it as follows choose Process gt Filter menu and click Morphological property page and a dialog will pop up with parameters as shown in the figure below on the left side Click OK and the final smoothed image is shown below on the right side 117 Help Manual Filters Image Enhanol Sedge Enhance Morphological Kernel Filters Sample O Erode O Gradient O Dilate Distance 8 Open O Thinning 1 Close _ Watershed O
37. should be run first in order to measure objects in terms of units other than pixels Resolution setting x Fixels per meter k at 3780 Cancel LE 3780 I keep the resolution in and direction equal X Horizontal PPM Pixels per meter of current Resolution Y Vertical PPM Pixels per meter of current Resolution Note The resolution in the Y direction need not to be filled ToupView will always let it equal to the X direction After the new Resolution is set all of the measurements will be calculated according to the new Resolution See more in the Measurements and Options gt Measurementeee menu 80 Help Manual 12 Process 12 1 Filtereee Shift F Choose the Process gt Filter command to apply one of ToupView s numerous filter on the active image If you are not familiar with the process and effects of filtering some discussions about spatial filtering should be reviewed ToupView provides an extensive set of convolution and no convolution morphological filters you can also create custom filter kernels and apply them with the Filter commands Choosing the Process gt Filter command will open the Filter dialog Each group of filters has its own property page or tab where the Filter type and size can be selected The filtered results are almost always written to the active image The Edit gt Undo command can be used to remove Filter operations that have been applied 12 1 1 Image Enhance Filters x
38. than 2 this button will be enable Cancel Click to cancel the stitching process 4 images for stitching 91 Help Manual Stitching finished image In the stitched image there are some black areas these are happened when there are translations between the images in both directions 12 2 3 Browse Window Stitch Clicking the Folders Sidebar to activate it Double clicking the right directory to show the images under the directory in the Browse window Choosing the image files to be stitched and then clicking the right mouse button will display the context menu choosing the Stitch submenu to start the image stitch process One can also choosing Process gt Stitch to do the same operation after the files are selected in the Browse window 92 Help Manual O ToupView CES File Edit View Process Options Window Help Be a A B Centimeter cm y 40x v 100 Ml a aM loml rx z2 74GZl doed0 Oe Folders vaga b x 690 5 e i EZ Desktop A X Delete File Delete 1 3 Libraries Y H Homegroup Hi a B touptek Icons 5j Computer E CCA gt h la Local Disk C oa E Su Local Disk D Properties 2013 09 28 Ef Feihong E i ImagePro io nem y Bca E Fol Oun Ba Fm E Measurement Sheet H 33 After the Stitch command is selected a dialog called Stitch will popup Choosing the right direction and clicking OK to begin the stitching
39. will be chose as ToupView File Type tft by ToupView Sample The final file name is shown at the right of the Sample label for reference Show the rename file dialog The file name can be renamed according to this item When this item is checked a Rename dialog will pop up after choosing File gt Quick Save command or click the Quick Save icon on the toolbar The new name can be specified again according to the requirement Rename EN Cancel 15 1 2 File Page One can check a file Extension for the specified file Format and its Abbr abbreviation to determine whether or not it will be displayed in the Browse window or not The checked Format can be displayed in the Browse window Preferences Quick Save Extension Format Abbr Browse A tft TFT TFT y bmp Windows Bitmap BMP Y Print dib Windows Bitmap BMP Y rle Windows Bitmap BMP Y Rulers and Grids R a ee e O TE Cursor jpeg JPEG JPEG Y jpe JPEG JPEG Y Capture jif JPEG JPEG y Misc jfif JPEG JPEG Y png Portable Network Graphics PNG Y tif Tag Image File Format TIFF Y tiff Tag Image File Format TIFF Y gif CompuServe GIF GIF Y pcx PCX PCX Y Y o A Cancel Apply Used to identify the file extension 156 Help Manual English full name of file formats File Format abbreviation Browse Check uncheck to determine whether or not the file format can
40. 10 cut the full weight of the filter a value of 1 indicates that only 10 of the difference between the filtered pixel value and the original pixel value should be applied a value of 2 indicates that 20 of the difference should be applied and so forth 2 If Sobel or Roberts is checked no options are available 12 1 3 Morphological Ee xi Image Enhance Edge Enhance Morphological Kernel Filters Sample Erode Gradient wey Dilate Distance Open Thinning C Close Watershed C Top hat C Well M Options 2x2 Square 3x3 Cross 11x 11 Circle C 3x1Row C 5x5 Circle Passes E 1x3 Colomn O 7x7 Circle coca nom Check this morphological filter if one wants to modify the size of objects in the image The Erode filter erodes the edges of bright objects and enlarges the edges of dark ones Dilate Check this morphological filter if one wants to modify the size of objects in the image The Dilation filter dilates bright objects and erodes dark ones Open Check this morphological filter if one wants to modify the shape of objects in the image Assuming the image contains bright objects on a dark field 85 Help Manual the Open filter will smooth object contours separate narrowly connected a a r Check this morphological filter if one wants to modify the shape of the objects in the image Assuming the image contains bright objects on a dark field the Clo
41. 16 Vertical 17 Rectangle 18 RoundRect 19 Ellipse 20 Circle 21 Annulus 22 Two Circles 23 Arc 24 Text 25 Polygon 26 Calibration for both video image window 27 Gray Calibration for video window 28 Manual Fusion for video window Help Manual 5 Camera Sidebar The Camera Sidebar is used for the control of ToupCam camera it includes 12 groups The group can be expanded by clicking the group name or clicking the Down Arrow at the right of the group name Camera List Capture 4 Resolution dExposure 4 Gain fw White Balance WColor Adjustment Frame Rate 8Color Gray hFlip Sampling y Power Frequency Histogram we The down arrow to expanded the group 5 1 Camera List Camera List List the cameras connected to the computer that A support the Amscope application Click the camera name will start the camera video window 5 2 Capture amp Resolution group a Capture amp Resolution Snap Continuously Snap images by clicking it la Snap O Record Record Record video stream in wmv asf or avi Live 896 684 format 3584 2748 Live Set the video resolution Snap Set the Snap resolution for the image capture process 5 3 Exposure Gain group Gd Exposure amp Gain 1 When the Exposure amp Gain group is expanded Auto Exposure Exposure Target a green rectangle marked with Exposure will be overlaid on the video This marked regio
42. 16 Exit Choosing the File gt Exit command will close video all of the image windows and Browse window and remove their windows from the screen After all of the windows are closed ToupView will end itself Note If an image has been modified before attempting to Exit it ToupView will issue a warning to ask if user want to save the image or not first 27 Help Manual 7 Edit 7 1 Undo Redo Ctrl Z If the Image or Processing menu s submenu command is exerted on the image the Edit gt Undo menu will be enabled Most of the operations in ToupView can be undone Alternatively one can restore all or part of an image to its last saved version The basic Undo demo process is 1 Choose the File gt Open Imageeee command to open an image 2 Choosing the Image gt Adjust gt Auto Level command then the Edit gt Undo menu will be enabled Undo Redo ax Cu Index ation oO 1 Open 2 Auto Level 3 Choosing the Edit gt Undo command will cancel the Image gt Auto Level operation and return the Operation to its initial opened state Undo Redo a x S Cu Index ration 1 Open If return to the Edit menu one will find that the Edit gt Undo menu now is changed to Edit gt Redo You can select one of these two operations to understand the Undo and Redo mechanisms 1 Choosing the Edit gt Redo command will return the image to the Image gt Auto Level Operation and the Edit gt Undo menu will be ena
43. 4 check 60HZ will delete the rolling dark band for the 60HZ light fluctuation 5 For DC power no light fluctuation is existed and no compensation is needed 5 10 Sampling group 1 Bin Pixel binning refers to the method of e combining averaging pixels of blocks of da neighboring same color pixels to resize the video the lower resolution 2 Skip Also called Decimation means that a certain amount of pixels are not read out but skipped horizontally vertically or in both axes This reduces resolution of the resulting video but introduces subsampling artifacts 5 11 Histogram group 1 A Histogram illustrates how pixels in an 24 Histogram image are distributed by graphing the number of pixels at each color intensity level The Histogram shows detail in the shadows shown in the left part of the histogram midtones shown in the middle and highlights shown in the right part A Histogram can help you determine whether an image has enough detail to make a good correction 2 This group shows the Histogram of current active video Two vertical line markers show the upper and lower limits of the intensity levels These markers can be dragged with mouse If you are looking at a color image the Histogram will reflect the RGB red green and blue channels histogram at the same time R red G green and B blue values with lines of the same color 3 You can also enter directly the desired values in the L
44. 4 7 Rectangle 0 1 Move mouse to the 1st point click its left mouse button to mark it 2 Move mouse to the 2ed point click its left mouse button to mark it A Rectangle will be overlaid on the image according these two diagonal points Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 8 RoundRect 9 1 Use Arc object to determine the round size Its size is 21 39X2 42 8 2 Choose the Options gt Measurementeee command click Round Rectangle to set the Round x and y to 43 and 43 respectively 3 Move mouse to the 1st point click its left mouse button to mark it 147 Help Manual 4 Move mouse to the 2nd point click its left mouse button to mark it a round rectangle with Rri and its width and height the in the two dimensions will be displayed Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here
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46. 7 Add OK Cancel Delete Apply Name Scale Symbol Add Unit 10 Cancel dm 1 Scale is the ratio between meter and the specific unit For example Meter by millimeter is 1000 which can also be expressed as scientific notation 1e3 Meter by kilometer is 0 001 which is noted as 1e 3 2 Symbol such as m for meter cm for centimetre Add User can also define his own Unit Clicking Addeee button and the Add Unit dialog will be brought up as above Here one can enter the unit Name and Symbol in their fields Here we enter a name called Decimeter and its Symbol is dm its Scale for Meter by Decimeter is 10 Click OK to end the Add Unit operation or Cancel to cancel the Add Unit operation The final Length Unit list looks like the figure below You can find the Type is now named User This means that this Unit is not defined by the System but by the User General Angle Unit Sheet Object Length Unit Measurement Unit Current Symbol Type Scale Pixel px System NA Nanometer nm System 1e 009 Micrometer um System 1e 006 Millimeter Y mm System 1000 Centimeter cm System 100 Meter m System 1 Inch in System 39 37 Decimeter dm User 10 Add Delete OK Cancel Apply 15 2 3 Angle Unit page 164 Help Manual On the Angle Unit page one can select Radian Degree or PI as the Angle Unit M
47. AA AR 51 9 4 2 Video Overlay Market asrriria A A AA A a oo 52 9 5 Video Watermark e ee AAA 53 ooo Move WatSrma k eotecde oeiee criar a e E a io Moa L Move LO PS gin tee dla dul oda laa lb dia le dos do de do os tee eee o 55 9 6 2 Move EO LEDO soe aus sre Bud ane are Sie See Bie Sed SiS Rem ie 018 Soe Bom Que BU Soe Bie A Been 55 Col a ROta te Watermark peaches iD A E E ee J9 Voted oi TOSES To o a a a a a a e a e a a a a a a e a a eee eee 53 9 dal ROLALS TO ZODO erroneo a O a e a a a a a a a a a ee ce 56 9 8 Gray Calibrationsee susana aaa A A A AS AA 56 9 9 Vid o Source Pr pertys er a es 57 LO Video Stream EEES AAA A IEA IA a a a IRA IA IAEA 57 Oeil Still image Options SS e o 64 64 eaa eae aae aa a 5 ee anaa 58 10 Copr O ee ee II II a o III IA 60 10 1 Capture Image Fe Seven beer dd bee A AA A A A A 60 10 2 Time lapse Auto Capture P o occocoooooooooooononononononono 60 10 3 Start Recorde e A A a eee ee eee ee eee eee 61 LOLS Start Broadcast AAA a a a a a aa a B aaa a aa a aaa a a aa a B aa a E 64 11 nn 67 Jin MOUS o oso Sere reese eres eoe eee mee ener es meee Sere mee ee ons oes 67 11 1 1 Color Qua antizes te gos aea a ea a eee ene eee ee Se AAA A 67 daa Gray DOVER AE E AE A ARE E ARA EE AE A 67 Loe LOINC EEG ee eeeeeeee NN NANA 67 nn ee ee ee ee ee ee ee 67 dhe 2e2 AUTO level sa e a ene ok tue Sek ake Sue Sie See Gee Oye See Sek Oud See ee Gees ore Bums 69 slo AUCO CONCE robaba beba 70 11 2 4 Hi
48. Contents ToupView Help Manual Capture amp Resolution Group EDS E Exposure amp Gain g LOUP eserse Er AE A eaaa eau ee ui eat White Balance Croup ss essa IA AAA Color Adjustment Group 22626266666666666566665605656665CF65CF656 Frame Rate GFOUP cassis natni dde dedos da dad da da ds e dos dd a do Flip group 0 ei Co JJ WD UO A W NP Color Oray Grell osu seis e cate eae eae aa 9 Power Frequency group 2262454620626 bee OnG EERO a EERE E ee Bee LO Sano lino Group ger me ne a ea aN aana aN eN aN aN aN a aN a aN a aN aN a aN a aN aN ee N E A eo ep o o A RR EE E E E A ao 01 oa a oO 0 0 0 a GO Oo Oo el Paramebets QEOUO tests i ddd bd ie Sd ad doi 1 Open Images ia caca hh E ica ica dra ica io 2 Open Video BE oi a a A A A A A A REESE EE ES vo Open BrocUcasee s door iodo iD A A AA teed ACA AAA Orv 01 OD OO 4 Camera List ee caeaea enue ea A ER a A Contents 6 5 Twain Select Devices irrarr dar daraian a da aA A 17 Ceo Twain Pere ee bobo O Been O A O Bere A E Eee 17 6 7 Save Mm Cll escocesa raros arenosa 19 Cle RR RR RN 20 649 Batch Savesrss ei A A E Ok Be A aiaa 23 6 10 Quick Save CTREFQ autora aaa 25 6a LE Paste as New Pile qomen trine RRE 25 Gel Print Setupess 2424266646264 A A A A A A A aa a a aa A aa Ea a ES 26 6 13 Print Previewees Age eds tee eee ee eee ee ee ee AAAE 26 6 14 Printeee Sci 2acececee ee acacia 26 Cade Recent Elles soodonadordss desolada sados dedos ss d 26 Ge LO EXILE sine sne dri dne aui
49. DO5000KPA 1 2 32 32 File gt Twain Select Deviceeee command first this will invoke a dialog called Select Source as shown below Source Choose the right device from the Source window Highlight it Select Click Select to select the device User does not need to repeat this step for subsequent choosing of the Twain Acquireeee command 6 6 Twain Acquireeee Introduction There are basically two techniques used to capture the video images from video 17 Help Manual devices such as a PC camera digital camera and scanner They are the Twain Acquireeee technique and the DirectShow technique previously called VFW The most obvious characteristics of the Twain technique is that it previews the video in smaller resolution but captures the image with higher resolution The USCMOS and UHCCD series cameras support all of these two video preview techniques Steps for Twain Acquire Here we illustrate how to capture the image using a UHCCDO1400KPA 1 4M pixels USB2 0 camera as an example 1 Install the camera driver for example driver for UHCCDO1400KPA hardware 2 Install ToupView 3 Plug the cameras UHCCDO1400KPA USB2 0 into the computer 4 Start ToupView 5 Choose the File gt Twain Select Deviceeee command to select the device UHCCDO1400KPA from the Select Source dialog If never selected before 6 Choose the File gt Twain Acquireeee command There should be a dialog box like below 18
50. General Define the columns of the measurement sheet Length Unit 7 in a P viN Angle Unit lar Sheet Diameter Down v Area Object Length Default Angle Start Y End Y Distance Cancel Apply 15 2 5 Object page The Measurement Object page list the Point Line Parallel Two Parallels Vertical Angle Rectangle Circle Arc Annulus Two Circles Ellipse Polygon Scale Bar Text characteristics Their characteristics can be modified in this page according to the requirement Different Object has different characteristics Measurement EJ Cene Set the default properties of the measurement object Length Unit E Poin A Label Type Coordinates gt Size 8 iii Line Color Ml co3eff Sheet E Lin Label Type Length Line Width 0 Line Color Ml 110000 Line Style A A A A A A K Arrowl Arrow2 E Parallel Label Type Distance Line Width 0 Line Color E ff 00c0 Line Style E Two Parallels Label Type Distance Line Width 0 Line Color Ml 00080 M OK Cancel Apply Click Defaults can return the all the modifications to the Default one 15 3 Magnificationseee Choose the Options gt Magnificationeee command to manage the calibrated Magnification If one has defined the 4x 10X 40X 100X Magnifications the Magnification dialog 166 Help Manual should look like Magnification EJ Name Resolution OK 4x 20480 10X 51200 Cancel 40X 204800 Clear All 1 Highlight the Magnificatio
51. Image Enhance Edge Enhance Morphological Kernel Filter Sample C 5x5 Passes 1 Low Pass C Sharpness High Pass Flatten Gauss Median High Gauss Rank Equalization Option 3x3 1 Strength A Der 1 Undo Cancel Apply Low Pass Check this filter to soften an image by eliminating high frequency information this has the effect of blurring sharp edges The Low Pass filter replaces the center pixel with the mean value in its neighborhood The Low Pass filter can also be used to remove noise High Pass Check this filter to enhance high frequency information The High Pass filter replaces the center pixel with a convolved value that significantly increases its contrast from its neighbors The High Pass filter leaves only elements of high contrast Check this filter to soften an image by eliminating high frequency information using a Gauss function This has the effect of blurring sharp edges The operation of the Gauss filter is similar to the Low Pass filter but Help Manual Ll it degrades the image less than the Low Pass filter Check this filter to enhance fine details This operation is similar to the unsharp masking technique see the Sharpen filter but it introduces less noise into the image It uses a Gaussian curve type of kernel Available in 7x7 and 9x9 kernel sizes Equaliza This filter is used to enhance the contrast based on th
52. Importeee Choose this command to load a Measurement files measurement and overlay the Measurement Objects over the current video image background 8 2 2 File Save Choose this command to save the Measurement Objects over the current video image on the Current Layer to a Measurement files measurement 8 2 3 Export gt To Html File Export all the Layer Measurement Objects to the html file in tabbed format Layer Name Index Name Center Radius Area Length Angle Start End Distance Layer 1 1 L1 837 00 627 00 712 12 58 32 650 00 930 00 1024 00 324 00 l Rl 1268 00 400 00 259072 00 2176 00 1636 00 576 00 900 00 224 00 en 2 R2 1755 00 1110 00 186416 00 1740 00 1946 00 1354 00 1564 00 866 00 Length Unit P Angle Unit Pi Note This menu will be enabled only when there are Measurement Objects over the Background Layer video image 8 2 4 Export to Excel If this command is chosen the Current Layer Measurement Objects over the video image will be exported to Microsoft Excel The Objects parameters in the Measurement Sheet will also be arranged as a table A B E D E F G H p Name Center Radius Area Length Angle Start End Distance R1 1268 00 400 00 259072 2176 1636 00 576 00 900 00 224 00 R2 1755 00 1110 00 186416 1740 1946 00 1354 00 1564 00 866 00 Note This menu will be enabled only when there are Measurement Objects on the Current Layer video image 8 2 5 Auto High
53. Invert Cu Index ration 1 Open oO 2 Auto Level O 3 Invert j 7 4 Cut Ctri X The Edit gt Cut menu will be enabled only when an Object or Objects on the Layer over the image is or are selected Check the Measurements gt Object Select M or Edit gt Select All menu about how to select Layer objects for the Edit gt Cut menu function Choose the Edit gt Cut command to copy the selected Measurement Objects to the clipboard and delete the selected Objects on the image Any data already exist on the clipboard will be replaced The data copied to the clipboard can be pasted into the active window or into another opened video image window on the layer above the image using the Edit gt Paste command when there is no Layer over the Background create a new Layer with the Layer gt Neweee command first Note The Edit gt Cut command does not support Background Layer image Cut operation 7 5 Copy Ctrl C When the Measurement Object s is are highlighted the Edit gt Copy menu will be 31 Help Manual enabled When an image ROI is selected and the Current Layer is the Background Layer the Edit gt Copy menu will be enabled Choose the Edit gt Copy command to Copy the highlighed Objects on Measurement Layer or an image s selected area on the Background Layer to the clipboard Copy the selected area on the background layer to the clipboard 1 Select the image area using the Image Select E button on th
54. Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 10 3 Circle gt Three Points 9 Choose the Measurements gt Circle gt Three Points command to draw a circle with Three Points method on the specified layer Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 11 Annulus 1 Find the Annulus center 1 and click mouse button to mark the Annulus center 2 Move mouse to let the first circle superpose with the image circle clicking the mouse left button 3 Move mouse to let the second circle superpose the image circle clicking the left mouse button Two radiuses with number and the units will be displayed on the Annulus 150 Help Manual Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its
55. Preference gt eee command and clicking the Rulers and Grids page can set Ruler Color Cursor Color of the Horizontal Ruler and Vertical Ruler for the video or image windows The Grid Style Line Style and Line Color for the Grid overlaid on the video and image window can also be set Preferences Quick Save Ruler Color l i i File Cursor Color a Default Print Grid Style Manual Grid v Cursor Line Style Solid Capture Line Color Default Misc Cancel Apply Ruler Color The Horizontal Ruler color and Vertical Ruler color Default is blue 0 0 255 Cursor Color The Cursor color When you are moving your mouse over the video image window there will be Horizontal Cursor and Vertical Cursor move on the Horizontal Ruler and Vertical Ruler moving at the same time indicating the current Cursor position in both directions Default is Red 255 0 0 Grid Style The Grid Style can be No Grid Auto Grid or Manual Grid Default is No Grid Grid Line Style The Line Style for the grid can be Solid Dash Dot or DashDot et al Default is Solid Grid Line Color The color of the grid line Default is Red 255 0 0 15 1 5 Cursor Page This command will set the mouse cursor for the video and image window operations 159 Help Manual Preferences Quick Save Cross Horizontal None y Single File Double 1 Pixel Double 3 Pixels Print Vertical None v Double 5 Pixels Double 7 Pixels
56. Setup gt Start Pause command again to start the video 9 2 Full Screen ESC Choosing the Setup gt Full Screen command will display the video window in full screen style Choosing the Setup gt Full Screen command again will return to the default video window Note The window should be in focus to make the Setup gt Full Screen or ESC command enabled 9 3 View Propertieseee The Setup gt View Propertyeee will help you to understand the camera statistical properties Choose the Setup gt View Propertyeee command to invoke the View Property dialog as below a vier Properties x Device ID device sw 860BB310 5D01 Device Name UCMOSO00350KPA Still Image Cap N Video Width 640 Video Height 480 Compression RGB Bits Per Pixel Time second Frame Actual Frame R The items in the dialog are described in the following table Unique ID to identify the camera device Device Name Human readable string to identify the name of the camera device 50 Help Manual Still Image Capture Whether or not the camera supports Still Image Capture Still Image Capture is used for high resolution camera to capture an image with a different resolution from the video This feature is mainly used to capture high resolution image under low resolution video to compromise the frame speed and the image resolution Display Width The video window width Display Height The video window height Note The Actual Frame Rate is lis
57. Stitch Direction Horizontal y Co cae The final stitched image will look like below 12 3 EDFeee Shift E 12 3 1 Browse Window EDF Clicking the Folders Sidebar to activate the Browse Window Double clicking the directory to display the images in the Browse window in Icon mode Choosing the image files to be fused with Ctrl A Select all in the current directory or Ctrl left mouse button to select the image one by one and then clicking the right mouse button will display a context menu choosing the EDF submenu to start the image fusion process One can also choose Process gt EDF command to do the same operation after the image files are selected in the Browse window 93 Help Manual ToupView O File Edit View Browse Setup Capture Image Process Layer Measurements Options Window Help a zi ad a Pixel Folders 7 568 4 oole EDORO3 A R J EDOF_04 J EDOF_05 Open JL EDOF_06 EDOF_07 JL EDOF_08 JL EDOF_09 JL EDOF_10 JL EDOF_11 L EDOF_12 L EDOF_13 JL EDOF_14 JL EDOF_15 pol EL EDOF_17 v lt gt Uca E ro O un Btay Fme EJ Measurement Sheet na Hio MUI ek 7 4 1 D00 0d X Delete File Sort Icons Shift T E E E 0 1 0 1 0 A AA AA Shift F 24 bpp 1600 x 1071 Bal 13 13 After the EDF command is chosen a dialog called Select Method is pop up as follows Select Method Maximum Contrast v
58. This set up dialog is just the same as those of video record process set up dialog It mainly including Encoder Encode Parameter such as Bitrate Quality Key Frame Spacing in second et al Encoder EE Encoder Windows Media Video 9 y Encode Parameter Bitrate Kbps 1536 Defaults Quality 1 100 100 5 Key Frames Spacing 1 Second s lt tc coca Clicking the Next gt button again will invoke the Start to Broadcast dialog Here one can set the broadcast Time Limit in minutes The Encoder parameters are also listed in the Summary window for reference Clicking the Finish button will start the broadcasting process and it will end up the process when it reaches to the Time Limit 65 Help Manual Start to Broadcast _ Time Limit Minutes 1 2 Summary Encoder Windows Media Video 9 Quality 100 Bitrate 1536 Kbps Key Frames Spacing 1 Second s aa ee See File gt Open Broadcast to understand the Start Broadcast function 66 Help Manual 11 Image 11 1 Mode 11 1 1 Color Quantizeeee The Color Quantizeeee command is widely used to change the image bit ToupView supports the mutual changes among 24 bit 8 bit 4bit or 1 bit images When the Colour Bits dialog is opened the default x Color Bits checked color bit is the current image s color bit 24 bit Check the desired bit and click OK to end the com command The image will converted to the selected bs C ibit color bits
59. Tolerance color range into the target and thus acquiring continuous segmentation colorizing When some undesirable pixels have been added into the segmentation data the De Colorizing Pipette can be used to wipe off the undesirable pixels This operation process is the same as Colorizing Pipette that is to set the average color value of the clicked pixel s Mask Area as Basic Value and wipe off the segmentation data which is 115 Help Manual within the Basic Value Tolerance color range Colorizing Pipette Collect average color value of the clicked pixel s Mask Area as Basic Value and add pixels which are within Basic Value Tolerance color range into the segmentation data De Colorizing Pipette Collect average color value of the clicked pixel s Mask Area as Basic Value and wipe off the segmentation data which is within the Basic Value Tolerance color range Mask Area When Colorizing Pipette or De Colorizing Pipette is used to click the image a Mask Area is also needed to calculate the average color value of the clicked pixel The Mask Area s color value will be used as Basic Value Color values within the Basic Value Tolerance range can be added to or wiped off from the segmentation data The default Mask Area is 3 3 other options are Ly 575 7 7 RGB The average RGB values of the Mask Area Tolerance There are 3 Tolerance values they are R G and B User can make fast and accurate object segmentation by selecting a
60. Type Index v orga am de w sa Y Area mm Minimum 30 Maximum IRA r a e o om 0 V Perimeter mm gt qe o o ee o Minimum 49 Maximum 12 4 2 OTSU Darkeee OTSU is proposed in 1979 by Japanese scholars which is a method to determine the threshold of an adaptive also known as Otsu method referred to as OTSU It is based on the gradation characteristic of the image and divided the image into the background and the target The bigger the OTSU is between background and the target the larger difference between the two parts images The wrong part of the the background or the wrong part of the target will lead to smaller differences between the two parts Therefore the maximum OTSU means the minimum probability of misclassification OTSU Dark segmentation method means segment the dark object from the bright background using OTSU algorithm Its Count Options dialog is exactly the same with the Watershed Count Options Below are bright background with dark object bottom left and its segmentation result bottom right for reference ra FE gt AE gt P O amp 8 d 0 ES of m E 7 J E y 8 a ll 3 E i SE 3 12 4 3 OTSU Bright B eee OTSU Bright segmentation method means segment the bright object from the dark d 106 Help Manual background using OTSU algorithm Its Count Options dialog is exactly the same with the Watershed Count Options Below are dark background wit
61. UCMOSO3100KPA Here a 3 1M List pixel camera is installed command to create the video window Camera Sidebar Click the Camera Sidebar If it is not activated and the Camera List to expand the Camera List group if not expanded Click the camera name Here it is UCMOSO3100KPA to create the video window B Camera List UCMOSO3100KPA Help Manual 2 Video window GUI 2 1 Video window GUI AB x Close Close All But This lose All But This Floating File Properties Docking ES seks Tabbed Document Copy Full Path AC Auto Hide Browse Containing Folder Close File Properties Copy Full Path Browse Containing Folder Hide Open Containing Folder Close All But This Open Containing Folder A ToupView Video UCMOS00350KPA SS UCMOS00350KPA GS Capture amp Resolution Exposure amp Gain White Balance Color Adjustment 5 Frame Rate a Color Gray Ab Flip ol Sampling 9 Power Frequency 2 Histogram 4 4 4 4 4 4 4 4 4 4 E F G Camera Folders BundoRedo Bayer F Measurement i E Measurement Sheet N UCMOS00350KPA Frame Rate 13 1 Frame 2244 J poosossea Cw AF a ai ON view Properties x Measurement Lo E Genera General Device ID device sw 860BB310 5D01 11D0 Device Name UCMOS00350KPA ico Sill Image Captire N Measurement Sheet Video Width 640 PT Object Video Height 480 Compression RGB FOURCC Bits Per Pixel 24 Time second 630
62. a second pass Strength Enter an applied value from 1 10 that reflects how much of the filtering Help Manual effect on the image A value of 10 specifies the full strength 100 of the filtered result applied to each pixel Values less than 10 cut the full weight of the filter A value of 1 indicates that only 10 of the difference between the filtered pixel value and the original pixel value should be applied a value of 2 indicates that 20 of the difference should be applied and so forth This value specifies which pixel in the sorted array will be used to replace the center pixel Pixels in the array will be sorted in ascending order The pixels are indexed from O to Kernel Size x Kernel Size 1 In the pixel index O corresponds to the lowest pixel value The Rank will be specified in terms of a percentage of the indexes Kernel Size x Kernel Size 1 A 50 Rank means the middle of the array 0 rank means the lowest index lowest gray value and 100 rank means the highest index highest gray value 2 If the Equalization filter is checked the options will relate to the histogram equalization Local Histogram Equalization modifies the contrast of an image based on the pixel values in a small window surrounding each pixel Window Image pixels statistics min max histogram mean standard deviation etc will be calculated on a small Window of the image These measurements are then used to derive the local contrast
63. a Properties dialog as shown below 48 Help Manual The file Properties dialog including 4 pages They are General Security Details and Previous Versions pages These Property pages may depend on the operating system and we will not discuss it in this help manual FI E99_of_frog_400Xjpg Properties ES MBS E00_of_frog_400Xjpg Properties xi Object name E help DemoPicture Egg_of_frog_400Xjpg ES Eag_of_frog_400Xjpg AAA SSS Group or user names Type of fle JPEG ipa FR Authenticated Users 5 A SYSTEM Opens with toupview exe Change 2 Administrators TOUPTEK S06 Administrators 2 Users TOUPTEK S06 Users Location ENhelp DemoPicture Size 511 KB 524 194 bytes Size on disk 512 KB 524 288 bytes Created Tuesday April 17 2012 9 59 49 AM Modified Friday March 23 2012 3 34 46 PM Accessed Tuesday April 17 2012 9 59 49 AM Attributes J Read only Hidden ZIT CIT General Securty Details Previous Versions General Security Details Previous Versions 7 4 ee ee ee ee Windows Backup How do use previous versions There are no previous versions available 3 21 2012 1 42 AM Program name Adobe Photoshop CS Wind Dimensions 1360 x 1024 Width 1360 pixels y Open Copy Restore 49 Help Manual 9 Setup 9 1 Start Pause Pause If the window is running one can choose the Setup gt Start Pause command to pause the video and choose the
64. ad BS Ok Sek Se Se Bee Gn ua Oe OG HS EOE SL ERE Sx LIS Close ALL sensis A ee eoe TEE HI RS RA AAA AENA Ika Reset Minas NOEL wene keai din wee un NAAA O ARA a 174 Windowst ee yw am se EAN RANA EINEN He HR Ewe AA 174 AM e a a athe aim E aime rana Sine ag tance PI a a e Ri 176 Help CONTESTES wos i woe Oe ES Bk AAA BASAN AAA ANAIS 176 Nol i ee ee ee E L76 Levenhuk Inc 1935 Brandon Court Suite A 1 Chicago IL 60139 USA Levenhuk is a registered trademark of Levenhuk Inc 2006 2015 Levenhuk Inc All rights reserved 20141029 Help Manual 1 How to start 1 1 Start 1 Double click on the desktop icon O to start ToupView 2 Click the Start button At your screen bottom left corner and a Start menu will bring up Move your mouse over the menu and try to locate ToupView click to start it 1 2 Create the camera video window ToupView will detect all of the cameras that your computer has installed Here it is UCMOSO3100KPA a 3 1M pixel CMOS camera and will append all the camera names as submenu to the File gt Camera List menu Here the submenu name is UCMOSO3100KPA Choosing the File gt Camera List gt UCMOSO3100KPA will create a video window and start the video stream The video window will be associated with the name of Video UCMOS03100KPA i e its title bar name will be Video UCMOSO3100KPA There are 3 methods to start the video stream they are File gt Camera Choose the File gt Camera List gt
65. added new Object on the Current Layer 13 9 Show Hideeee Set the non current Layer visible or invisible The Current Layer cannot be toggled between Visible and invisible state It is always Visible 13 10 Renameeee Rename a Layer 13 11 Export to Image Export all the layers Measurement Objects to the image this will pollute the image and cannot be recovered anymore 13 12 Export to Microsoft Excel Export the image plus all the Objects on the Current Layer to the Excel file 140 Help Manual 14 Measurements The Measurements menu is mainly used for the image measurement application With this menu items you can measure the image with many geometrical shape at ease ToupView use Layer technique to perform the measurement operations This will never pollute the image pixels The Measurements menus and its submenus are shown below Y arbitrary Line D ERS Sa Horizontal Line E B Z angle Vertical Line F C Point Line gt G Z Parallel X Four Points I H Two Parallels O AA Vertical gt K C Rectangle Center Radius O L O RoundRect Two Points p Ellipse O Three Points Q Circle gt R Annulus Tro Circles i Center Radius S U MM arc Three Points V Text W x Polygon ra Top X Z Order gt 2 Bottom Y a a Move up F 4 Y Move down AA About the Layer technique please check the Layer menu and Layer Sidebar in Sec 13 About the Measurements setup pl
66. all of files under the Folders Sidebar directory which is just as the Edit gt Select All command 7 11 Delete File Delete The Edit gt Delete File menu will be enabled if the file s is are highlighted in the Browse window This command is for Browse window only You can Delete or remove one or more files from the Browse window The steps are as follows 1 Select one or more files by a Clicking the displayed file icons a single file will be 35 Help Manual highlighted b Clicking the file one by one with CTRL left mouse button all of the clicked files will be highlighted c Clicking the displayed file icons the first clicked file will be highlighted clicking the end file with Shift left mouse button all of the files among the first and last will be highlighted d Dragging the mouse to draw a dotted line rectangle across the files you wish to delete all of the files in the rectangle will be highlighted 2 a Press the Delete key to delete the selected files b Click you right mouse button to bring up a context menu choose the Delete command to delete the highlighted files A Confirm File Delete dialog will bring up In the Confirm File Delete dialog click Yes to move the files to the desktop recycle bin or No to cancel the Delete operation 36 Help Manual S View 8 1 Browse Ctri B 8 1 1 Open the browse window 1 Choose the View gt Browse menu from the View menu or click the Browse toolbar button E
67. an be Solid Dash Dot or DashDot et al Default is Solid Grid Line Color The color of the grid line Default is Red 255 0 0 8 8 Best Fit Choose the View gt Best Fit command to automatically resize the video image to fit in the window Note Choosing this command will enable View gt Actual Size menu 8 9 Actual Size Note This option will be disabled if the image is currently viewed at 100 At any other zoom ratio View gt Actual Size will be enabled Choose the View gt Actual Size command to set the active image to its Actual Size e g 100 8 10 Track E Note If the video image size is smaller than the window size The track operation will be disabled If the image s actual size is larger than the video image window check this command to position the video image that does not fit entirely within in the video image window Its function is similar to the scroll bars It is an alternative to using the arrows on the scroll bars for positioning the video image within the window Checking the View gt Track menu will change the cursor to fi and the button on the toolbar will be checked Then keep down the mouse button to drag the region of interest on the video image to any location in the video image window 8 11 Properties If an image file listed in the Browse window is highlighted Choosing the View gt Properties command or clicking the selected file in the Browse window with the right mouse button will bring up
68. ates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 4 3 Line gt Vertical Line Draw a Vertical Line between the two points on the specified layer When the first point is marked the second point s x coordinate will be restricted to equal to the first point s x coordinate automatically Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated 144 Help Manual automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 5 Parallel 1 Choose the Measurements gt Parallel command 2 Move mouse and click to mark the 1st point 3 Move mouse and click to mark the 2nd point 4 Move mouse and click to mark the 3rd point 5 Move mouse again one will find the 4th point is always restricted to parallel to the line connecting point 1 and point 2 Click to mark the 4th point Tw
69. ay of week 00 53 x Date representation for current locale X Time representation for current locale y Year without century as decimal number 00 99 Y Year with century as decimal number z Z Time zone name or abbreviation no characters if time zone is unknown Yo Percent sign For example Choosing amp f Date Y m d will print the file name and Date with yyyy mm dd format on the page header Choosing Page amp p amp P will print Page 01 11 on the page footer Suppose the current page is page 1 and the total pages is 11 Print the measurement item If there are measurement item overlaid on the image check it will print these objects on the image Measurement Sheet on the page Print the measurement table If there are measurement item overlaid on the image check it will print these objects Measurement Sheet on the page with Always use another paper Print the Measurement Sheet data on a separate page Auto Print the Measurement Sheet data just behind the image if the image and data can be arrange in a single page of on a separate page if not Do not use another paper Print the Measurement Sheet data just behind the image page Font Choose the Font for the data 15 1 4 Rulers and Grids Page 158 Help Manual Choosing the View gt Ruler and Grid gt Settingeee command or choosing the Preference gt eee command and clicking the Rulers and Grids page realize the same function Choosing the
70. be displayed in the Browse window the File gt Save As dialog Config To configure the image file Save As Option for JPEG Portable Network Graphics Tag Image File Format CompuServ JPEG 2000 Standard JPEG Codestream For details please refer to the Option button explanation in 15 1 3 Print Page One can set the Page Header and Page Footer in following format Preferences ES Quick Save Page Header AB amp p amp P File 7 amp f HHH Y m d Print Page Footer FAA m Rulers and Grids v Print the measurement items Cursor W Print the measurement table Capture Page Usage Always use another paper v Misc Microsoft YaHei UI 9pt Font amp f File Name amp p Current Page amp P Total Pages A Full weekday name b Abbreviated month name B Full month name c Date and time representation appropriate for locale d Day of month as decimal number 01 31 H Hour in 24 hour format 00 23 I Hour in 12 hour format 01 12 j Day of year as decimal number 001 366 m Month as decimal number 01 12 157 Help Manual M Minute as decimal number 00 59 p Current local times A M P M indicator for 12 hour clock S Second as decimal number 00 59 U Week of year as decimal number with Sunday as first day of week 00 53 ow Weekday as decimal number 0 6 Sunday is 0 W Week of year as decimal number with Monday as first d
71. bled again 2 Choosing the Image gt Auto Contrast command then the Edit gt Redo menu will change to Edit gt Undo 28 Help Manual Note ToupView supports only one step Undo and Redo operations 7 2 Forward The Edit gt Forward menu will be enabled if it is not in the final step in the Undo Redo Sidebar The Edit gt Backward menu will be enabled if it is not in the first step in the Undo Redo Sidebar The Edit gt Forward command will forward the current displayed image to the next operation listed in the Undo Redo Sidebar If it is not in the final step Forward Demo 1 Open an image choose the Image gt Adjust gt Auto Level and the Image gt Adjust gt Invert commands check Current on Index 1 Open Operation The Undo Redo Sidebar is shown below in Fig 1 4a Cu Index ration 1 Open O 2 Auto Level O 3 Invert Undo Redo x 3 Cu Index ation oO 1 Open 2 Auto Level O 3 Invert Undo Redo E3 Cu Index ration O 1 Open O 2 Auto Level 3 Invert Now we continue the Edit gt Forward menu demo Since it is in Index 1 the Edit gt Backward menu is disabled and the Edit gt Forward menu is enabled 2 Choosing the Edit gt Forward command the image and the Index will Forward to 29 Help Manual Fig 2 Now the Edit gt Backward menu is enabled 3 Choose the Edit gt Forward menu again to go to Index 3 as shown in Fig 3 Now the Edit gt Backward menu is still enabled but because it is in t
72. bmp kongdong bmp Libraries Computer Network File name SS Save Save as type Windows Bitmap bmp dib re v Cancel If Save or Cancel is clicked in the Save As dialog ToupView will return to the Save 173 Help Manual Files dialog to continue the next file 2 Clicking No on Save Files dialog will close the current image window without saving operation and return to the Save Files dialog to continue to the next file 3 Clicking Cancel will cancel the Save Files dialog without doing anything 4 Clicking Yes to All on Save Files dialog will always bring up Save As dialog to let you enter the File name to save files one by one until all of the files are saved 5 Clicking No to All on Save Files dialog will close all of the image windows without saving them 6 Clicking Batch Save on Save Files dialog will transfer the save process to File gt Batch Saveeee command Please check the File gt Batch Saveeee menu for details Note If you have a lot of pictures opened and have made some modifications on them and want to close them quickly without saving anything you may uses this command and choose Step 5 All the images will be closed instantly no warning will be given 16 2 Reset Window Layout Choosing this command will reset the ToupView window layout to the original one The reset will be effective after restart ToupView ES A The window layout will be reset after restart ToupView 16 3 Windowse e e Choosing the
73. button context menu 3 2 How to close the image window Help Manual 1 Tabbed window a If you have modified an image before attempting to close it double clicking the tabbed image window title or clicking x on P X will bring up a ToupView dialog x O Image has been modified Need save _ o e Clicking Yes will save the changes with its old name and close the window quickly No will close the file immediately with no changes and no warning or Cancel will cancel the Close command and leave the window there with no changes b If the Image window is snapped from the video window and with number as its title double clicking the tabbed image window title or clicking x on x will bring up a warning ToupView dialog Toupview x O Image has been modified Need save _ o e Clicking Yes will bring up the Save As dialog xi Save Ji Deore H opem No items match your search Recent Places Fiename IO M Save as type Windows Bitmap _bmp dib fe y Cancel Select the driver and folder to which you want your image file saved in the Save in combobox and enter the file name in the File name edit box Help Manual Click Save to save the captured image with the specified directory and file name or Cancel to close the Save As dialog and return to image window Clicking No on the ToupView dialog will close the file immediately with no changes and no warning or Cancel on t
74. casts By default this feature clips the white and black pixels by 0 5 that is it ignores 0 5 of the lightest pixels and 0 5 of the darkest pixels when identifying the lightest and darkest pixels on the image Choose the Options gt Auto Correctioneee command to modify this default setting This ensures that white and black values are representative without being determined by extreme pixel values The Auto gt Correctioneee dialog is shown below Auto Range xj Auto Level Low High 0 005 0 005 Auto Contrast Cancel High Low 0 005 4 0 005 The Image gt Adjustment gt Auto Level command gives good results when an image with an average distribution of pixel values needs a simple contrast adjustment or when an image has an overall color cast However adjusting the Curves manually is more precise See Image gt Adjustment gt Auto Contrast for another auto adjust command 11 2 3 Auto Contrast The Image gt Adjustment gt Auto Contrast command automatically adjusts the overall contrast and mixture of colors in an RGB image Since it does not adjust channels individually Auto Contrast does not introduce or remove color casts It maps the lightest and darkest pixels in the image to white and black which makes highlights appear lighter and shadows appear darker When identifying the lightest and darkest pixels on an image Auto Contrast clips the white and black pixels by 0 5 that is it ignores
75. cking 44 on the toolbar a red line with pixels number and 0 000um length is overlaid on the video window At the same time a dialog called Calibrate will be displayed over the video window Calibrate Magnification Actual Length Cancel 512 000 Micrometer tom Resolution Pixel Meter Millimeter mm Centimeter cm Meter m Pixel Inch in Actual Length 6 Try to align the two ends of the red line with the video ruler scale Try to pull the red line as long as possible to keep the calibration accuracy 7 Enter or select the current microscope objective lens Magnification in the Magnification field The current Magnification is 10X 8 Read the overlaid by the red line micro ruler actual length and fill it in the Actual Length field the current Actual Length is 450um This Actual Length will also be displayed in the middle of the red line just behind the pixel number At the same time the Resolution will be calculated and displayed simultaneously in the Resolution field 168 Help Manual ls Magnification oK Pixel 574 001 Resolution 1275557 Pixel Meter Pixel Actual Length 9 If everything is ok click Ok to end the calibration The Magnification 10X This is the number you entered in the Magnification edit box will be available in the mo El Magnification dropdown combobox on the video window toolbar mM 10 If you wish to make the mea
76. cts within the background objects 130 Help Manual Background allows the other inputs to be displayed in front of the background inputs minimizing color mixing For example ToupView can display a red dot on a blue background without turning the dot to magenta Selecting None causes all images to be equally mixed New Click on the New button to discard the current color composite image and start over The Source Images combo box will reset to include all gray scale images including floating point images Selecting an image from the list will create a new composite the same size as the initial selection As mentioned previously the source images list will be filtered to contain only images of the same size as the initial selection 12 7 Rangeeee Shift R The Process gt Rangeeee command allows set the intensity levels of the image to increase the contrast and enhance the display in low light situations Choose the Range command to open the Range dialog Two vertical markers show the upper and lower limits of the intensity levels These markers can be moved with mouse through the drag and drop method For a color image the histogram will reflect the red green and blue values with corresponding colors lines Two Edit controls indicate the values of the intensity levels Choose the spin buttons to increase or decrease these values All values between 0 and the lower limit will be black and all values between the
77. d items will depend on the selected Object type The type could be the same or different 14 10 Circle 14 10 1 Circle gt Center Radius o Choose the Measurements gt Circle gt Center Radius command to draw a circle with Center Radius method on the specified Layer Its radius is C1 33583 88um Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 10 2 Circle gt Two Points Choose the Measurements gt Circle gt Two Points command to draw a circle with Two 149 Help Manual Points method on the specified layer Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected
78. deo file E help DemoPicture Browse Tip Ea wmv or avi will be appended automatically to the file name cso Te co 3 An Encoder dialog will be brought up Here you can select the Encoder format set the Bitrat Kbps 256 16384 Quality 1 100 and Key Frames Spacing 1 30 Click Back to return to the Video Format dialog or Next to the next step 62 Help Manual Encoder Encoder M Encode Parameter Bitrate Kbps 1536 z Default Quality 1 100 100 Key Frames Spacing 4 Second s 4 A dialog called Display Information will be brought up Here you can enter Title Author Copyright and Description to their fields Click Back to return to the Encoder dialog or Next to the next step xi Tite MR Author ToupTek Tip All the display information is optional ms Ce ca 4 A dialog called Start to Capture will be brought up Here you can check Time Limit Minutes 1 1440 and enter recording time If checked Input Time lapse 1 100 There is a Summary text to display what you have been defined Click Back to return to the Encoder dialog or Finish to end the setup 63 Help Manual Start to Capture AS xi Y Time Limit Minutes ll TimeJapse 1 4 M Summary Format wmv asf File E help DemoPicture TT wmv Encoder Windows Media Video 9 ty 100 Bitrate 1536 Kbps Key Frames Spacing 4 Second s O Record 5
79. djust gt Auto Contrast menus The default value is 0 5 for both but ToupView suggests this value be smaller than 1 Auto Range EA Auto Level Low Clipping High Clipping 0 0050 S 0 005 v Cancel Auto Contrast Low Clipping High Clipping 0 005 0 005 A See Image gt Adjust gt Auto Level and Image gt Adjust gt Auto Contrast for details 172 Help Manual 16 Windows 16 1 Close All Choosing the Closes All command to close all of the pictures opened or captured inside ToupView frame If you have made any modifications to the pictures or if you have captured some pictures from the camera choosing the Close All will let you finish the saving operations quickly Video Vwindow Captured pictures Opened amp modified If the above image windows exist choosing the Windows gt Close All command will bring up a Save Files dialog as below Save Files Ea File has been modified Do you want to save changes Yes No Cancel Yes to All No to All Batch Save 1 Clicking Yes on the Save Files dialog will close the Video and Browse window Image windows that are not changed directly without ask anything If there are newly created Image window ToupView will bring up a Save As dialog to prompt you to enter the File name to save file T Save As ES Savein TEST 7 et TRS A gt s Ss Recent places er ws b Pe at A e A Et f PA Oe Rae Puree stone Mm mee ess ko Desktop Blood bmp cell2
80. e Rulers and Grids O Office 2007 Silver A O Visual Studio 2005 Office 2003 8 Office XP O Visual Studio 97 4 Language Take effect after restarting ToupView O Default 8 English French Francais O German Deutsch O Polish Polski Russian Pycckuii Misc 6 9 Batch Saveeee Simplified Chinese 78114 PX O Traditional Chinese HEP X O Turkish T rk e 1 Privacy The maximum number of Recent Files 4 Clear the Recent Files when exit the application Y Restore the current directory when startup Cancel The File gt Batch Saveeee menu will be enabled when a an image is opened b an image is captured from the camera c an image window is created by choosing the File gt Paste as New File menu d an image window is created copied from the 23 Help Manual Undo Redo Sidebar Batch Save for the a style image window 1 If the image is modified choosing the File gt Batch Saveeee command will save the file with its opened file name and file extension 2 If the image is modified because of the Measurement Objects choosing the File gt Batch Saveeee menu will save the file with its opened file name but with ToupView File Type tft file extension format Batch Save for the b c or d style image window If the image window has been created with b c or d style choosing the File gt Save Aseee command to realize the file saving task will be tim
81. e as the reference image 124 Help Manual Note 1 ToupView support wmv asf avi mp4 m4v 3gp 392 3gp2 3gpp mov mkv flv rm rmvb for Image Stacking operation 2 In the Image Stacking process ToupView takes the first frame as the reference image this means the first frame is very important and should be assure the first frame to be the right image scene and the subsequent frames have a greater overlap region with the first frame 12 6 Color Compositeeee Use the Color Composite dialog box to create and configure color composites using black and white source images You can access the Color Composite dialog box through the Color Composite menu item from the Process menu 125 Help Manual xi Source Images Adjust DemoGrn TIF y C All Selected Registration Contrast B E G Colors I Lt DemoBlue TIF i EIB O Y 0 80 90 50 SetColor Bestrit Reset Background El E You can combine gray scale images into a color composite Any group of gray scale images that are of the same size can be mixed in a color composite Images of 8 12 16 bit integer or floating point format are combined into a 24 bit color composite Each input channel will have individual LUT adjustments as well as a registration offset to line it up with the rest of the images Color Composite 3 E xj Source Images Adjust DemoRed TIF y C all Selected cogen Cors B E G Colors
82. e consuming The Batch Saveeee command will runs File gt Save Aseee command with the name automatically specified according to the paradigm specified in the Batch Save dialog To start the File gt Batch Saveeee command you have to 1 Start the camera 2 Snap at least an image first 3 Choosing the File gt Batch Saveeee command will bring up a Batch Save dialog x _ Cancel File Name Format vyyy mm dd HH MM SS File Prefix TT File type png z Option Sample TT 2012 04 18 00 41 43 png Directory Enter the name of the drive and directory where your captured images will be saved You may either type the path information or use the Browse button to locate it from a standard Browse Folder dialog Name Format The year month date hour minute and second or nnnn sequence 24 Help Manual are used to as part of the file name If more files are saved with in a second a xx suffix is attached to the end of Name Format to avoid the possible name conflict For the nnnn sequence Name Format no suffix is needed File Prefix Enter a file name prefix for Batch Save when generating files names for a series of images This prefix will be combined with Name Format to form a final file name naming paradigm File Type In this combobox select the format in which you want the image to be saved can be BMP JPG PNG TIF Click Option button to set the different parameters for encoding the
83. e histogram of the tion local neighborhood See Option below Sharpness Check this filter to enhance fine details or refocus an image that is blurred The sharpen filter sharpens the image using the unsharp masking technique Median Check this filter to remove impulse noise from an image The Median filter replaces the center pixel with the Median value in its neighborhood It will also blur the image Rank Check this filter to remove impulse noise from an image The pixels in the kernel are ranked by order of intensity and the pixel in that range at the rank percentage is chosen for comparison For example in a 5x5 kernel there are 25 pixels A rank percentage of 95 would choose second brightest pixel for comparison If the difference between the selected pixel and the center pixel is greater than the threshold value the Rank filter replaces the value of the center pixel with the value of the selected pixel Option 1 If one of the Enhancement filters is checked the following options will be displayed Check 3 x 3 kernel will produce a more subtle filtering effect Check 5 x 5 kernel will produce a moderate filtering effect Check 7 x 7 kernel will produces a more extreme filtering effect Passes Set the filter applied times on the image When a filter is applied multiple times its effect is amplified by each pass An image that has been softened by one pass of the Low Pass filter will be softened further by
84. e toolbar The Edit gt Copy menu will be enabled 2 Choose the Edit gt Copy command to copy the selected image area to the clipboard Copy object s on the measurement layer to the clipboard 1 For the Layer operation see the View gt Sidebar gt Layer menu in Sec 8 and the Layer menu in Sec 13 for details 2 For the Measurement operation see the View gt Sidebar gt Measurement menu in Sec 8 and the Measurements menu in Sec 14 3 After the Measurement operating has been done choose Measurements gt Object Select command or check the Object Select button IN the cursor will change to Lx 4 Move the mouse until the cursor becomes tise this means the cursor is right on the Object Clicking it will highlight Object and select the Object aS 5 Option 1 Continue to move the mouse until the cursor becomes again this means the cursor is right on another Object again Clicking it with SHIFT left mouse button and the second Object will be selected and highlighted 6 Option 2 a Move the cursor over the image click down the left mouse button b Drag the mouse to draw a rectangle on the image A dotted rectangle will appear around the selected area c Release the mouse and all of the Measurement Objects within the dotted rectangle will be highlighted and selected 7 After the Measurement Objects are selected the Edit gt Copy menu will be enabled 32 Help Manual 8 Choose the Edit gt Copy to Copy the Object s
85. ease check Options gt Measurement and Measurement Sidebar in View gt Sidebar menu in Sec 8 About the Measurements menu s toolbar icons please check ToupView s toolbar in Sec 4 14 1 Object Select x The Measurements gt Object Select menu or the toolbar button will be enabled only when a new Measurement is made over the Background Layer After the Measurement is done on the specific layer choose this menu to select the Objects 141 Help Manual 1 The Object can be selected by clicking on it 2 Select a group of Objects by including them in a rectangular area with Object Select command or by press down the Shift key and clicking the Object with left mouse button until all the desired Objects are selected Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 2 Angle 1 Move the mouse on the image to point 1 mark it by clicking the left mouse button 2 Move the mouse to point 2 mark it by clicking the left mouse button
86. easurement EJ g General Degree y Radian Name tae Label Type Length Length Unit Line Width 0 Y Show all the angle units in the measurement sidebar Line Color E ff0000 Line Style _ Sheet Arrowl Arrow2 Object g Start 27 54 7 42 End 14 50 18 75 Length 17 27 Slope 0 87 Angle Radian 0 72 Angle Degree 40 99 Angle Pi 0 23 E Start x 564 Start Y 152 End X 297 Cancel Apply End Y 384 Show all the angle units in the measurement sidebar will list all the Object Units in the Measurement Sidebar as shown above for reference 15 2 4 Sheet page See the View gt Measurement Sheeteee menu to understand its functions 1 To modify the Measurement Sheet s item order click the item to highlight it the Up or Down buttons will be enabled If the item is in the third position the Up button will be disabled if the item is in the last position the Down button will be disabled Click the Up or Down buttons to modify the item position Note The item Index and Name are always in grayed states this means these two items cannot be modified Both their hide show states and their positions 2 Checking Unchecking the item will show hide the item in the Measurement Sheet 3 Clicking Default will return to the ToupView s default settings 4 Both the name and order for Item 1 Index and item 2 Name cannot be changed 165 Help Manual Measurement EJ
87. ebar in Sec 5 for details 8 6 3 Sidebar gt Folders The Folders Sidebar is mainly used for the image Browse control Checking the View gt Sidebar gt Folders menu will activate show the Folders Sidebar Clicking its tree can navigate the file directories 43 Help Manual Double clicking the directory in the Folders will create the Browse window If there are image files under the tree that ToupView supports to browse the image files will be displayed in Large or Small icons mode Their order can be set in Forward or Reverse format according to Sort by Name Type Size Width or Height et al Clicking with the right mouse button on the directory will bring up the right mouse context menu as shown below MZ Desktop E HL Capture Effect cd D E help d EE COCE cht Mouse Button METAS Context Men H megroup Read Y gt oo ane ai Homegroup Read Write Dy open H Libraries Add to Windows Media Player list Specific people E amp Homegroup Play with Windows Media Player H A ZHAOLEI EE A A qe Restore previous versions al DATA D Indude in library gt ae Cy El Ea DATA E Send to gt sic Cut Copy Pictures Egvideos 1 Create new library Create shortcut i HyperSnap_6 HS Hj office2010 El Jo tupian Ay RECOVERY F 43 DVD Drive G Properties Bluetooth device E UP H m i Network 146 che pressed zipp
88. ecial and some explanations are needed here to clarify it in details 1 The function provides users with five methods which are Watershed W OTSU Dark OTSU Bright RGB Histogram HSV Histogram and Color Cube Users can select any one of those 5 methods but after selecting any one of them the others will be disabled 2 After segmentation there may exist adhesions Then what we should do is segment connected object manually by choosing the Split Objects function 3 When the users get the expected results they can choose the Counting Results menu and get the statistics and analysis results According to the counting result you can go back from step 2 or 3 to step 1 or 2 to repeat segmentation or split objects operation 102 Help Manual Note If the users choose None in the Label button at the Count Options dialog then regardless of the type of Method set there is no Label for Index Area or Perimeter on the image But the None Circle and Ellipse will show the segmentation results That s why ToupView name the menu as Segmentation amp Count That is this function can achieve either the image Segmentation or Count In this regard please refer to the Process gt Segmentation amp Count gt Watershed W in the Count Options dialog 12 4 1 Watershed W eee Watershed segmentation method is a mathematical morphology segmentation method which based on topological theory The basic idea is taking the image as a topological ge
89. ed folder H E Control Panel EZ Desktop create shortcut E Recyde Bin g Documents ToupCam Samples Fax recipient Mail recipient en UP H This is the basic window explorer menu and will not explain it at here in this manual 8 6 4 Sidebar gt Undo Redo The Undo Redo Sidebar is used to Undo Redo operations for the Image and Process menus command Checking the View gt Sidebar gt Undo Redo menu will activate show the Undo Redo Sidebar 8 6 5 Sidebar gt Layer Layer Sidebar is used for the management of Layer operations This operations including making a New Layer Removing a Layer or Renaming a Layer and Layer visibility controlling et al Checking View gt Sidebar gt Layer will activate show the Layer Sidebar 44 Help Manual 8 6 6 Sidebar gt Measurement The Measurement Sidebar is used to check or edit the selected Layer Objects on the Layer Checking View gt Sidebar gt Measurement will activate show the Measurement Sidebar In this sidebar the Object Appearance Calculation and Coordinates characteristic are listed The Appearance and Coordinates can be edited 8 7 Ruler and Grid Ruler and Grid menu has 3 submenus they are 8 7 1 Grids gt No Grids Choosing this command will remove both Manual Grids and Auto Grids overlaid on the video image window 8 7 2 Grids gt Manual Grids Choosing this command will display two small Right Arrow and Down Arrow overlaid on the top of the Vert
90. eft or Right boxes below the Histogram chart for both Left and Right Histogram boundaries 4 Click the Refresh button to update the Histogram display if the sample under observation is moved or changed 5 Clicking the Defaults button will return the Left and Right Histogram boundaries to its original ones 11 Help Manual 6 Click the Auto button to locate the two boundaries automatically to get the best video quality 5 12 Parameters group ee Parameters group is used to save the camera control parameters adjusted for further application Click Save will save the current camera control parameters with new parameter file The Load and Overwrite buttons will be enabled Click Load will load the saved camera control parameters Click Overwrite will overwrite the current camera control parameters to the current loaded active camera control parameters file Click Management will invoke a Management dialog and in this dialog one can manage all of the saved parameter files 12 Help Manual 6 File 6 1 Open Imageeee a Ctri O Choose the File gt Open Imageeee E command to open an existing image file The Open Imageeee command can also be used to preview an image in small size or to view its statistics and information without actually opening the image itself These capabilities can be used to quickly locate a particular image ToupView supports and can open many image formats These are identified in the Fi
91. elected The relative position of the object and the microscope should be changed in one direction 5 Unless there is 1 captured image the Clear buttons will not be enabled Unless there are more than 2 captured images the Next gt buttons will not be enabled If the captured images are not satisfactory click the Clear button to clear the captured image s and capture new image s 6 If more than 2 images are captured Clicking Cancel will end the Capture Image and return to the Video window If the Next gt button is clicked ToupView will pop up a dialog called Selected Method to begin the EDF configuration process About the configuration detail please check Browse Window EDF in Sec 12 3 1 The final fused image will be displayed in a new active image window and its title bar will be associated with a digit assigned by ToupView 101 Help Manual Note a Use the coarse and fine focus knobs to move the sample stage up and down b Clicking the Capture button will capture the current image into the image fusion list c Repeating steps 1 4 until there are enough images d Clicking the Fusion button will start image fusion Waiting for some time and a fantastic fusion result will be displayed in a new image window 12 4 Segmentation Counte e e Choose the Process gt Segmentation amp Count command to realize the segmentation and count of the interested image The arrangement of Segmentation amp Count is very sp
92. elow 57 Help Manual xi Size 640 480 320 240 One can change the video format mode frame rate color space compression options and so on Here we only have the Video Size to check Selecting the desired one and clicking OK This will give you a selected Video Size for the new video window Click Cancel to cancel the selection and return to video window without any changes or Apply to apply the current selection to the video with the Properties remain opened Note Different devices may have different user interfaces and items Contact the camera supplier for details 9 11 Still Image Optionseee Some cameras can capture a still image with its sizes different from the video stream The camera may have a pin that acts as a hardware trigger or it may support software triggering A camera that supports still images capture will expose a still image pin to the designer To capture a still image one should first set the size of the image to capture The size is determined by the camera hardware Here we show an example with UCMOSO8000KPA camera Choosing the Setup gt Still Image Optionseee command will show the following Properties dialog 58 Help Manual xi Size 3264 2448 C 1600 1200 C a00 600 Cancel Apply It has 3264 2448 1600 1200 and 800 600 resolutions Select the desired one and press OK to accept the selection or Cancel to ignore the current selection Click Apply
93. ering the image rotation shifting and scaling between a series of images in the video The steps of Image Stacking are described as below 1 Choose Processing gt Image Stacking menu a Windows Open dialog will pop up as shown below 123 Help Manual Open EJ gt Computer gt Local Disk D TEST vw GQ Search TEST Organize y New folder gt i Y d banner A Feihong Music Photo test avi sw TEST ToupTek 2013 09 28 A1_product rrr e y A2_procureme A3_sales A4 help A5_PriceList A8 ToupTek Y pp o pr y y y Pe File name test avi v Video Files wmv asf avi V Choose the right video file recorded before in the Open dialog here we can open test avi for demonstration click Open to open the avi file Then an Image Stacking dialog will pop up to show the progress of the each image s stacking progress Image Stacking ES Cancel If Cancel is pressed the Image Stacking will be canceled After the stacking is finished a stacked image will be displayed in a new window for further application The final image is shown as below One can find the stacked image signal to noise ratio is greatly improved From the stacked image has some black edges this is because the images in the video is moved and in the stacking process ToupView will added O to the image area that has no corresponding pixel in the reference image we choose the first image in the video fil
94. ext gt button will be enabled clicking on the Next gt button will start EDF process ToupView will display the EDF Extended Depth of Focus progress bar as follows EDF Extended Depth of Focus oon M The final EDF result is shown below NOTE Images used for fusing must be the same size otherwise there will be a prompt when adding different sizes of images as below The prompt dialog indicates which image is not the same size with the others ToupYiew xi Image 2010 6 large jpg is not of the same size 12 3 3 Video Window EDF Make sure that the ToupView package and UCMOS or UHCCD camera are correctly installed Turn on the microscope s light 1 Run ToupView and start the camera 100 Help Manual 2 Choosing the Process gt EDFeee command or clicking Fl on the toolbar will bring up a dialog called Capture Image 3 Use the microscope coarse or fine focus knobs to move the sample stage up and down in order to find the positions where the clearest regions of the whole sample can be seen on the Video window 4 Click the Capture button to capture an image into the image list which will be used for the EDF operation Capture Image ES Captured 0 Capture Tips Adjust the microscope to make different part of the object clear Capture an image when the object is in different position The step of the adjustment is up to the users Typically more details will be available if smaller step is s
95. file For BMP format the Option will be disabled See File gt Save Aseee menu about the details of the format encoding methods If there are Measurement Objects over the image the File Type will be chose as ToupView File Type tft by ToupView Sample The final file name is shown at the right of the Sample label for reference 4 If everything was finished click OK button to begin the file batch save process or Cancel to cancel the File gt Batch Saveeee command and return to the application Note a In the process of the File gt Batch Saveeee command the title on the image tab or image window will be modified with the file name formed in the Batch Save dialog b The File gt Batch Saveeee will perform no saving operation if the file is not modified 6 10 Quick Save CTRL Q The File gt Quick Save menu will be enabled when a an new image captured from the camera b an image window is created by choosing the File gt Paste as New File menu File gt Quick Save can save the file at quick way with no need to specify the file directory file name and file format All those are specified in Options gt Preferenceseee Quick Save property page The file name can be renamed according to the setup in the Options gt Preferenceseee Quick Save property page s Show the rename dialog item 6 11 Paste as New File The File gt Paste as New File menu will be enabled when there is valid image data on 25 Help Manual the clipboard first
96. fixed layout flat document including the text fonts graphics and other information needed to display it In 1991 Adobe Systems co founder John Warnock outlined a system called Camelot that evolved into PDF While the PDF specification has been available free of charge since at least 2001 PDF was originally a proprietary format controlled by Adobe It was officially released as an open standard on July 1 2008 and published by the International Organization for Standardization as ISO 32000 1 2008 In 2008 Adobe published a Public Patent License to ISO 32000 1 granting royalty free rights for all patents owned by Adobe that are necessary to make use sell and distribute PDF compliant implementations If your computer still does not install the PDF reader please try to download from http www adobe com downloads 17 2 About Display the related information about ToupView including ToupView version compatibility built date and its developer s www etc About ToupView ES O ToupTek ToupView Copyright C 2003 2014 Version x64 3 7 3031 Compatible Windows XP Vista 7 8 Built Apr 25 2014 11 31 46 http www touptek com Clicking on the URL link will direct you to the address of the camera supplier If you have any problem with the camera or ToupView please feel free to contact your Supplier 176
97. for that area of the image In short an area of Window x Window around each pixel is all that is considered when modifying the intensities in the image Larger Window produces smoother results while small Window track small details more closely Best Fit Choose the Best Fit button to optimize the values for the particular image The results are achieved by stretching the local histogram to maximize the contrast between the brightest and darkest pixels in the local window region Linear This option distributes the histogram linearly across the intensity scale This function produces a high contrast image with the highest possible dynamic range Logarith This option concentrates the histogram at the low end of the scale This 83 Help Manual function produces a high contrast image with little dynamic image It will tend to darken the image overall It is useful for increasing the contrast in a very light image Exponen This option concentrates the histogram at the high end of the scale This tial function produces a high contrast image with little dynamic image It will tend to lighten the image overall It is useful for increasing the contrast in a very dark image 12 1 2 Edge Enhance Image Enhance Edge Enhance Morphological Kernel Filters Sobel Roberts Sculpt O Laplace C variance Horizontal Vertical Options cancel ay Check this filter to enhance just the
98. ft Vertical Line in histogram When input a Left Value in this edit box users should click enter to make the value effective Meanwhile the Left Vertical Line will move to the place the Left Value represents Left Value range O current Right Value The current Left Value is 0 Right Value in the currently selected region of histogram segmentation corresponding to Right Vertical Line position in histogram Users can segment the pixels bigger than the Left Value and smaller than Right Value by inputting a value in this edit box or dragging the Right Vertical Line in histogram When input a value in this edit box users should click enter to make the value effective Meanwhile the Right Vertical Line will move to the place the Right Value represents Right Value range current Left Value 255 The current Right Value is 79 A ost lil channel of the bio HSV histogram It can be H aes Show all channel HSV histogram When uncheck Show All only a e E Used to identify the Color of the EEE pixels The default is Options When users click Options button a Count Options dialog pops up as below The setting of Count Options dialog is the same as that of Waltershed s Count Options dialog details can be referred to Process gt Segmentation Count gt Watershed W 112 Help Manual Count Options Approximation None With holes Outline Style Outline Color m Default M Label Ty
99. g up the color dialog ae separately which allows select the start and end colors of the range MS 12 10 Surface Plote e e The Process gt Surface Ploteee or 3 D Plot tool creates a 3 D representation of the intensity of an image When choosing the Surface Plot command keep in mind that X image width and Y image height and Z pixel gray value In the viewpoint window the elevation and rotation of the image can be adjusted by dragging the mouse on the image 133 Help Manual 3 D Setup m Position Solid Image Background Color a M m Color Table Lo Position Solid The left edit control indicates the relative position of the entire image in the viewpoint window whose default value is 0 5 The right edit control indicates the relative height of the display of the Z scale whose default value is 1 Reset Set the Position Solid s two edit controls to their default values Image Background Color Choose this command to display a color dialog where one can adjust the background color of the viewpoint window Capture Capture the active image in the viewpoint window as a new image Color Table Select the colors to map the gray values found in the surface plot The button at each end of the color table brings up the color dialog which allows select the start and end colors of the range Please refer to Pseudo Color for more information 12 11 Line Profileeee Choose the Process gt Line Profileeee
100. gin the Time lapse capture or Cancel to cancel the Start Time Lapse Auto Capture e command After the Time lapse capture is started the Capture gt Start Time Lapse Auto Capture eee menu will be changed to the Capture gt Stop Time lapse Auto capture menu Choosing this command will stop the Time lapse capture There are a variety of image format available they are bmp jpg png and tif to save the captured image For example when choosing jpg format you can set the parameters of Option to adjust its compression quality or encoding method Please check the File gt Save Aseee menu for details 10 3 Start Recordeee F9 1 You can a choose the Capture gt Start Recordeee command b click Record button Oo J on the Camera Sidebar c use the shortcut key F9 to start recording movies The video format can be wmv asf recommended or avi 61 Help Manual Video Format ee m Please select the video format wmv asf recommended 2 Clicking Next on the Video Format dialog will bring up Video File dialog as below Enter the video file name under 1 Set the name for the captured video file field and click the Browse button under 2 Select the directory for the video file item to locate the video file directory Click Back to return to the Video Format dialog or Next to the next step Video rie a 1 Set the name for the captured video file rr 2 Select the directory for the vi
101. guration preserves the circular shape of the objects better than square configurations 2 If the Tophat Well or Gradient filter is selected the options will relate to kernel size and shape The following options will be presented 3x3 Check to use the 3x3 square kernel configurations SXI Check to use the 5x5 square kernel configurations PERS Check to use the 7x7 square kernel configurations 3 If Watershed Thinning or Distance filter is checked the options will relate to the threshold The following option will be presented Petes Enter a percentage value from 1 100 that specifies the intensity value to resho binarize the image For example a Threshold of 50 on a Gray Scale 87 Help Manual image would set all values lt 127 to O black and all values gt 128 to the maximum value for that image class white 12 1 4 Kernel The Kernel page allows edit the kernel files for the morphological and convolution filters Image Enhance Edge Enhance Morphological Kernel M Filter type Convolution Morphological Kernels higauss 7x7 Edit ES New ipass 5x eet a a ee Option Passes i Strength h Note The HiPass LowPass Laplacian and Unsharp kernel files are used by the HiPass LowPass Laplacian and Sharpen options listed in the Image Enhancement Filters page dialog window i e there is no difference between selecting one of these kernel files a
102. h bright object bottom left and its segmentation result bottom right for reference 12 4 4 RGB Histoman eee RGB Histogram segmentation is based on image histogram where image pixels between a specified histogram upper and lower range will be color processed The segmented area can be adjusted by mouse to drag two vertical lines in histogram or by inputting precise value in RGB upper and lower edit box directly and clicking Enter to make it effective The Color representing the segmentation area can be customized by system palette For a 24 bit true color image segmentation can be realized by R G B channels respectively Choose Process gt Segmentation amp Count gt RGB Histogrameee shows Histogram dialog each function is specified as below Histogram ES Cancel Options T E 7 E Green v Y Show All Left Value in the currently selected region of histogram segmentation corresponding to Left Vertical Line position in 107 LE AECI histogram Users can segment the pixels bigger than the Left value by inputting a value in this edit box or dragging the Left Vertical Line in histogram When input a Left Value in this edit box users should click enter to make the value effective Meanwhile the Left Vertical Line will move to the place the Left Value represents Left Value range O current Right Value The current Left Value is 0 Right Value in the currently selected region of histogram
103. he Fra me Ra te th at yo ur com p u te r U S B h u b fastest frame rate that the computer USB hub supports If live video fails to display move the slider to the left to decrease the frame rate supports If the video fails to display drag the slider to the left Low to reduce the Frame Rate and enable the video in a low speed mode 5 7 Flip group If the video on the screen appears in different A Flip iu directions from what is viewed under the camera check the Horizontal or Vertical box to set the video direction to the right one 5 8 Color Gray group A Color Gray If you wish to preview Color video select the Color Gray Color button If you wish to preview Gray video check the Gray button 5 9 Power Frequency group 1 A CMOS sensor captures each row of pixels Y Power fans 9 Ac 50 Hz from top to bottom in sequential order ae creating a rolling effect hence the name DC Rolling Shutter Instead of being relatively constant so for example as the commercial main frequency in Europe is 50Hz fluorescent lights in Europe flicker at 100 times per second and as the main frequency in US is 60Hz so in the USA they flicker at 120 times per second 2 This flickering problem is solved by capture row pixels in over the duration of integer number of n flicker periods Help Manual 3 check 50HZ will delete the rolling dark band for the 50HZ light fluctuation
104. he Maximum Angle increases and the relationship between them is non linear The default is 1 0 Clicking lt Back will return to Option dialog and clicking Finish will begin the EDF 96 Help Manual operating according to the previous setup ToupView will display the EDF Extended Depth of Focus progress bar as follow EDF Extended Depth of Focus oo The final EDF result is shown below e es Weighted Average The setups of the Weight Average are the same as those of the Maximum Contrast About the setup details please refer to Maximum Contrast in Sec 12 3 1 1 123 12 FFDSSD Choosing FFDSSD in the Select Method dialog will pop up a FFDSSD Options dialog as below Determine the reference image automatically Before EDF FFDSSD will evaluate all the images to determine the reference image for feature matching After the reference image is determined FFDSSD will perform feature matching between the reference image and the other images to find the alignment parameters The default is checked If the Determine the reference image automatically is unchecked ToupView will take the first image as reference image in the alignment 97 Help Manual operation FFDSSD Options Determine the reference image automatically Clicking Finish button will begin the EDF an EDF Extended Depth of Focus progress bar dialog will display as follows EDF Extended Depth of Focus on The final EDF result is sho
105. he ToupView dialog will cancel the Close command and return to image window Note Choosing the Windows gt Close All command can also close the tabbed image window Check the Windows gt Close All for detail 2 MDI window a For an opened and modified image window click X on la x to bring up the same ToupView dialog The next operations are just the same as the tabbed window in step 1a b For a Video captured image with number as its title clicking X on 0 x will bring up the same ToupView dialog The next operations are just the same as step 1b in the tabbed window Note Choosing the Windows gt Close All command will also close the MDI image windows in a quick way Check the Windows gt Close All for detail Help Manual 4 UI toolbar When the camera is started or the image is opened most of the icons on the toolbar will be available for the quick setup of the video or image characteristic 1 3 5 7 9 11 13 15 ii 1 21 23 25 27 dd Al Millimeter mr 4x 100 y mew RB Lew SH a LG OO Pen ty El 2 1 6 8 10 12 14 1 18 20 al 24 26 28 1 Open Ctrl O 2 Save Ctrl S 3 Quick Save Quick Save 4 Browse Ctrl B 5 Unit 6 Magnification 7 Zoom 8 Video Image Select 9 Track enabled only when the video image sizes large than the window size 10 Object Select will be enabled only when an object is existed on the layer above the background layer 11 Angle 12 Point 13 Line 14 Parallel 15 Two Parallel
106. he default Save as type will be ToupView File Type tft The Save As command has several important uses beyond simply storing an image to a new file name Click Option to select the different parameters to encode the file xl Best Compression quality Progressive Smoothing 0 4 I Optimize Huffman codes Save these settings as defaults Cancel Reset all For JPEG jpg jpeg jpe jif jfif Option has the following items 21 Help Manual If one save an image in JPEG format jpg one may Image quality adjust image quality in the edit box The values range from O to 100 Default value 75 The default is unchecked Optimize Huffman codes The default is unchecked Smoothing The values range between 0 and 100 Default value 0 Save these setting as When saving a file the current settings will be saved as defaults defaults for the next file save operation For Portable Network Graphics png Option has the following items Interlaced Save these settings as defaults Cancel Reset all Interlaced The default is unchecked Save these setting as When saving a file the current settings will be saved as defaults defaults for the next file save operation For Tag Image File Format tif tiff Option has the following items TIFF x 7 Append pages Compression Lzw default y M Image quality Base A A E A A Best Compression H qua
107. he final operation the Edit gt Forward menu is disabled See Edit gt Backward for further information 7 3 Backward The Edit gt Forward menu will be enabled if it is not the final operation in the Undo Redo Sidebar The Edit gt Backward menu will be enabled if it is not the first operation in the Undo Redo Sidebar The Edit gt Backward command will move the current displayed image to the previous Index listed in the Undo Redo Sidebar If it is not in the Open status Backward Demo 1 Choose the Open Image command to open an image 2 Exert the Image gt Adjust gt Auto Level command on the image 3 Choose the Image gt Adjust gt Invert command the final image is shown in Fig 3 Since it is in the final operation state Fig 3 the Edit gt Backward menu will be enabled 4 Choosing the Edit gt Backward command the image and the Index will Backward to Index 2 as shown in Fig 2 Since it is in Index 2 the Edit gt Forward menu will be enabled 5 Choosing the Edit gt Backward command again both the image and the Index will Backward to Index 1 as shown in Fig 1 Since it is in Index 1 the Edit gt Forward menu will still be enabled but the Edit gt Backward menu will be disabled See Edit gt Forward for further information 30 Help Manual Undo Redo x a gt 33 Cu Index Operation f oO 1 Open oO 2 Auto Level 3 Invert i i gt Undo Redo x O 1 Open 2 Auto Level O 3
108. ht is modified and vice versa Otherwise uncheck the Constrain Proportions button Scale method There are 3 options for the Scale method They are Nearest Neighbor Bilinear and Bicubic The default is Bilinear 11 6 Histogrameee A Histogram illustrates how pixels in an image are distributed by graphing the number of pixels at each color intensity level The Histogram shows whether the image contains enough detail in the Shadows shown in the left part of the Histogram Midtones shown in the middle and Highlights shown in the right part in order to make a good correction The Histogram also gives a quick picture of the tonal range of the image or the image key type A low key image has detail concentrated in the shadows a high key image has detail concentrated in the highlights and an average key image has detail concentrated in the Midtones An image with a full tonal range has a number of pixels in all areas Identifying the tonal range helps determine the appropriate tonal corrections 78 Help Manual Choose the Image gt Histogrameee command to open the Histogram dialog as shown below Depending on the image s color mode choose R G and B or Luminosity to view a composite Histogram of all the channels If the image is RGB true color choose Luminosity to display a Histogram representing the luminance or intensity values of the composite channel A xi channel gt ee pixels 352800 level
109. i dhe ara ahe ara ee ee eee arane 99 12 3 3 Video Window EDF Mis a a AA AAEE EE EE 100 12 4 Se egmentation amp COUNC aegea e aeae aa eb a ie aoa a A ee ees 102 Contents Zeta Waters nediM eee carac 103 12 442 OTSU Darks cee naren iver a aiee A A NA A A Ewer a aiea 106 12 4 3 OTSU Bright B cece care care care eareearetaretare cages 106 IZ ve hee Hie lOme AA eee COC CCC REECE CECE CSO RN 107 12 4 5 Eo tee os c h Bs JBs ee JB JB ee JB ee JB ee JB Ss JB ee JB JB fen JB JB ee JB Oe JBs JB J OE 111 LA Color Cubic te 5 oh 6 bdo ood mo oe ee be Oe eee OOo eee ae eee 115 Irae Tr OPE OE O O AS AS AS AE A E A 120 sta Count Result T Ses gogog a a a a A A A a a a 122 12 5 Image Stacking eee 26604686 see eRe Ree ERS Oe Oe ee ee eS 123 12 6 altar GompoSite sss 26 26 66 66 sara 66866086 CC a a a a a a a 125 12 7 Range SMC edad sad losa do alos a edo ad don 131 12 8 Binary OI Be sos eas ese bne bse ake bne Bre Be Gre are Bue Aim Bre ue Bra Aen ari 132 LA PScuUdo COOLS ES E SAD DI 132 Toreo S bam de PLOTS A goaa a e e a a a a a a o a a a a a E 33 IZ kk Line BEQ LISECES PETER ERA EA AAA A AAA AAA 134 TAS LA MOSCA LEER AAA A A A A AA AS ES 135 13 Layf ees boo ii a acia io a oda ia a aio 138 LL OE Mae E sia aa Ma 01 REG Je at tak 01 Jot Jas 0k JE 0 8a ae AE cat Je 0 aa an Oe OO 138 1342 Organizing Layers s errira iodo dd 66 56 6 04 araa ica 138 13 3 Layers for non destructive measurement and label 138 l
110. ical Ruler and on the left of the Horizontal Ruler as shown below tee u Move the mouse over the Down Arrow will show a horizontal drag icon Drag the Down Arrow along the Horizonta Ruler to where ever you want When it is dragging over the video image there will be a Vertical line appeared to let you judge where to 45 Help Manual release this line on the video image You can drag any lines to overlay them on the video image The overlaid lines can be adjusted by dragging the specific Down Arrow again on Horizontal Ruler Move the mouse over the Right Arrow will show a vertical drag icon Drag the Right Arrow along the Horizontal Ruler to where ever you want When it is dragging over the video image there will be a Horizontal line appeared to let you judge where to release this line on the video image You can drag any lines to overlay them on the video image The overlaid lines can be adjusted by dragging the specific Right Arrow again on Vertical Ruler Horizontal Manual Grid Choose the Grids gt No Grid command to remove the Manual Grids overlaid on the video image window The menu will be checked Choose the Grids gt Manual Grids command again If it is unchecked to display all the previous Manual Grids overlaid on the video image window The menu will be checked 8 7 3 Grids gt Auto Grids Choose the Grids gt Auto Grids command to overlay the grids on the video image automatically 46 Help
111. ifferent 14 4 Line 14 4 1 Line gt Arbitrary Line Draw Arbitrary Line between the two points on the specified layer 1 Choose the Measurements gt Line gt Arbitrary Line command 2 Move mouse to the 1st point click the left mouse to mark it 3 Move mouse to the 2nd point click the left mouse to mark it again a line with L1 and its length will be shown 143 Help Manual Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 4 2 Line gt Horizontal Line Draw a Horizontal Line between the two points on the specified layer When the first point is marked the second point s y coordinate will be restricted to equal to the first point s y coordinate automatically Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordin
112. image or the composite BCG sliders The Brightness Contrast and Gamma sliders are the similar to the Contrast Enhancement feature in ToupView You can also adjust each channel individually or collectively by using the Selected or All radio buttons to the left of the BCG sliders in the Adjust group box Best Fit Click this button to perform a Best Fit contrast stretch on the selected input automatically setting the Brightness and Contrast settings accordingly Gamma will be reset to 50 This method is identical to the Best Fit contrast adjustment under the standard Contrast Enhancement feature of ToupView Best Fit is useful for first pass adjustments Reset Pressing the Reset button automatically resets the BCG settings If the Adjust radio button is set on All the BCG settings for the overall color image will be reset Frame Use the frame slider to adjust the frame of the selected image that is used for the preview or the active frame of the composite Please see the discussion of Color Composite and Image Sequences that follows this section Background Select one of the available images to be the background color in this combo box The rest of the image planes are subtracted from the background image effectively punching holes into it and allowing the foreground colors to show without mixing with the background This is most effective where the background covers large areas and the other images have smaller obje
113. in the image window 11 1 2 Gray Scale Choose the Gray Scale command to convert a color image true color image or index color image to a gray scale image If the original image is 24 bit the new image is 8 bit Otherwise the bit of the image will not be modified 11 2 Adjust 11 2 1 Curveeee Choose the Image gt Adjust gt Curveeee command to adjust the entire tonal range of an image But instead of making adjustments using only three variables highlight shadow midtone one can adjust any point on the curve along a 0 255 scale while keeping up to any other values constant One can also use Curve to make precise adjustments for individual color channels on an image The Curve dialog is shown below Heighlights M s Midtones daad Shadows Y Grid M lel ml ho gl The horizontal axis of the graph represents the original intensity values of 67 Help Manual the pixels Input levels Vertical The vertical axis represents the new color values Output levels In the Axis default diagonal line all of the pixels have identical Input and Output values Curve Area specified with the blue rectangle to setup a map between input area and output a When you open the Curve dialog you see perhaps surprisingly not a curve but a straight line As a default the input values are the same as the output so there s no change b By clicking in the center of the line and dragging upward
114. io 2008 _ Office 2007 Luna Blue Office 2007 Obsidian Black _ Office 2007 Aqua Office 2007 Silver _ Visual Studio 2005 Office 2003 Office XP O Visual Studio 97 9 Language Take effect after restarting ToupView O Default English French Francais _ German Deutsch _ Polish Polski Russian Pycckuit Simplified Chinese 1 P32 O Traditional Chinese HPX O Turkish T rk e amp Privacy The maximum number of Recent Files 4 C Clear the Recent Files when exit the application Vi Restore the current directory when startup 15 2 Measurementseee This sheet has many pages They are 15 2 1 General page The General page is shown below Measurement Length Unit Angle Unit Sheet Object The calculation results keep 2 decimals v Label font size Default Label color Y Dimensions with unit 1 decimal Y Label with name 3 decimals 4 decimals 5 decimals 6 decimals Cancel o Cancel Apply 2 decimals Apply The calculation results keep The combo box allows you to set the calculation results 162 Help Manual accuracy among 1decimal and 6 decimals Default is 2 decimals Label font size The Label is used to display the Measurement Objects dimensions the Label font size is among 5 and 15 Default is 15 Label Color The Label Font color for the Measurement Objects dimens
115. ions Default is red Dimensions with unit Ifthis box is checked the Dimension will be displayed together with the unit for the Measurement Objects If unchecked only the Dimension will be displayed with the Measurement Objects Label with name Checking Label with name will add a Prefix before the Measurement Objects number The Prefix for the different Object can be specified in the Measurement Sidebar where there is an item called Name Here we show an example for Line Object with and without Prefix L1 as below Label with name checked Label with name unchecked 15 2 2 Length Unit page Length Unit page One can select the Length Unit for the Measurements menu s Objects on the Layer Measurement operation Unit The system unit defined by the ToupView It cannot be deleted Current The Unit selected or checked Type Type of unit It can be System Defined by ToupView or User Defined by the user type Scale Represents the ratio of Meter by unit For example if the unit is um then Meter by um should be 1000000 the Scale should be 1e 6 163 General Unit E Pixel Length Unit Nanometer Angle Unit Micrometer Millimeter Sheet Centimeter Object Meter Inch Measurement Current Symbol px nm pm Y mm cm m in Help Manual Type Scale System NA System 1e 009 System 1e 006 System 1000 System 100 System 1 System 39 3
116. is button to display real time effects when drags the slider bar Default is checked Brightness Dragging the slider bar to the left decreases the level and dragging it to the right increases the level The numbers on the right of the slider bar displays the Brightness value Values can range from 100 to 100 Default is 0 71 Help Manual Contrast Dragging the slider bar to the left decreases the level and dragging it to the right increases the level The numbers on the right of the slider bar displays the Contrast value Values can range from 100 to 100 Default is 0 11 2 6 Coloreee Choose the Image gt Adjustment gt Coloreee command to modify the overall mixture of the colors in an image There are four color modules ToupView uses the RGB model It assigns an intensity value to each pixel ranging from O black to 255 white for each of the RGB components in a color image For example a bright red color might have an R value of 246 a G value of 20 and a B value of 50 When the values of all three components are equal the result is a shade of neutral gray When the value of all components is 255 the result is pure white when the value is O pure black RGB images use three channels to reproduce up to 16 7 million colors on screen In addition to being the default mode for new ToupView images the RGB mode is used by computer monitors to display colors This means that when working in color modes other than RGB such a
117. it Segmentation amp Count Color Custom Color used to represent the color of Label When selecting XOR in the Method the Color selection will be disabled but the ToupView will automatically mark the segmented image with the current pixel s XOR color Type Including 3 options 1 Index use numbers to mark the segmented object 2 Area the object area is marked by outline area size 3 Perimeter the object perimeter is marked by the outline perimeter Minimum determine the lower limit of the area that is if the object area is less than the lower limit it will not be included Maximum determine the upper limit of the area that is if the object area is greater than the upper limit it will not be included Perimeter Minimum determine the lower limit of the perimeter that is if the object perimeter is less than the lower limit it will not be included Maximum determine the upper limit of the perimeter that is if the object perimeter is greater than the upper limit it will not be included The figure below shows image Segmentation amp Count results with watershed method with setting in Count Option dialog 105 Help Manual o e e Count Options ES 09 0 gt a A e o A Approximation Ellipse v With holes o gt 3 gt ES Ss 4 N Outline o y gt e Style Outline y Color Mera y Cancel e y 0 o e Label o es amp y a o Method True S Color N gt a gt
118. ithin blobs to the outer boundaries of those blobs After applying the distance filter the background will be black i e pixels with value 0 Only the area within the blobs will have non zero values will be white The values of each pixel within the blob will be a count of the shortest distance from that pixel to the edge of the blob Thus all pixels along the blob s border will have a value of 1 since they are one pixel away from the edge of the blob pixels that are a distance of 2 from the border will have the value 2 and so on Help Manual This creates a distance map of the image The Distance filter will not operate upon True Color images If one wants to use the Distance filter with a True Color image he must first convert it to Gray Scale Options 1 If Erode Dilate Open or Close filters is checked the options will relate to the kernel size and shape The following options will be presented Check to use the 7x7 circular kernel configurations This is a 7 x 7 Circle two pass filter accomplished using a 5 x 5 circle followed by a 3x3 cross Check to use the 11 x 11 circular kernel configurations This is a 11 x 11 Circle three pass filter accomplished using a 5 x 5 circle followed by another 5 x 5 circle followed by a 3 x 3 cross Set the number of times iterate the filter Note The circular kernels are especially effective on round objects cells grains and so on because their circular confi
119. ius major minor axis or non Area and Perimeter as is shown below The unit of statistical result is the same as what the user selects in the Unit for the displayed image Click Index Center Radius major minor axis or none Area and Perimeter and the statistical result will be automatically sorted according to their data Choosing a certain item can check the split object in the image In the meanwhile Index or Area and Perimeter of the other targets will be hidden automatically Result Index 0 N DU A UNA Center 108 82 96 30 51 32 95 94 119 61 95 57 60 67 94 36 37 57 90 30 29 81 89 42 66 49 85 19 25 06 89 02 82 24 93 40 15 70 84 66 13 58 82 36 12 70 76 54 41 45 80 95 2 65 78 66 14 29 72 84 83 97 76 58 105 80 81 50 17 99 70 02 aA AS 41 Radius 0 54 0 98 3 32 6 18 1 42 0 57 0 57 5 78 12 06 4 50 6 21 0 73 6 13 5 95 0 57 6 20 16 57 7 26 154 Area 0 25 0 75 12 82 59 16 0 25 0 31 0 06 76 52 173 82 1 62 53 00 0 62 60 03 64 70 0 19 66 13 309 87 82 86 nia Perimeter 2 41 4 11 20 68 41 91 5 69 2 62 2 62 45 86 101 48 20 56 44 11 3 41 52 35 40 29 2 62 55 76 189 77 62 48 Aan A co The Center column in Result is relevant to the Approximation option in the Options dialog When selecting Circle radius of the circle will be listed as Radius column and when choosing Ellipse major minor Axis of the E
120. ives superior results to FFDSSD method with deep stacks such as images produced by microscopy It is worth noting that images to be processed must be in Z order Capture a series images in closest to furthest or furthest to closest order FFDSSD FFDSSD is a method that does filtering in the frequency domain using Fast Fourier transform FFT and does synthesis in the space domain FFDSSD can be used for images having misalignment Users can manually or automatically select reference images to make images aligned 12 3 1 1 Maximum Contrast Choosing Maximum Contrast in the Select Method dialog will pop up an Options Dialog as follows Options ES Detail Clarity 107 Total Brightness 125 Background Brightness 93 Defaults Description The three values are not isolated Detail clarity affects the fine detail of the result Background brightness is the offset value that is used to add or subtract the background These two settings will result in the change of the total brightness For that reason the total brightness may also need to be adjusted Detail Clarity Detail Clarity affects the fine detail of the result Default 107 Range 10 240 Total Brightness Total Brightness is the overall brightness The Detail Clarity and Background Brightness will result in the change of the Total Brightness For that reason the Total Brightness may 95 Help Manual A also need to be adjusted Default 125 Range 10 240 Backgro
121. just the HL Highlight M Midtone and S Shadow parts of the image Each part s value ranges from 100 to 100 This command is only available for 24 bits true color image Preview Check this button to display the real time effect when one changes the Slider bar s position Default is checked 11 2 8 Gammaeee The Image gt Adjustment gt Gamma measures the brightness of midtone values produced by a device often a monitor A higher gamma value yields an overall darker image Preview Check this button to display the real time effects when one changes the Slider bar s position Default is checked Gamma Dragging the slider bar to the left decreases the level while moving it to the right increases the level Values can range from O to 3 0 Default is 1 0 11 2 9 Filter Coloreee Choose the Image gt Adjustment gt Filter Color command to filter a special color channel from a color image Check either Red or Green or Blue color to filter For every pixel if check Red color to filter only information about the red channel will be 74 Help Manual discarded and Green and Blue information will remain there Select Color xi pa ES OK Red C Green ie C Blue See Extract Coloreee for another color operation 11 2 10 Extract Coloreee Choose the Image gt Adjustment gt Extract Coloreee command to extract a special color channel from a color image Check either Red or Green or Blue
122. les of type list box You may also open an image file type with ToupView File Type tft format which has Measurement Objects overlaid on the image j x Look in i DemoPicture 025 Sse He ZA Recent Places oF A Ve 0001 jpg 0002 jpg 0008 jpg Butterfly_1 Butterfly_4 a AS bo tradh Ciliated_epit Ciliated_epit Ciliated_epit Dense_con Dense_con E 0 E E Dense_con Egg_of fro Egg_of fro Egg_of fro H E Tisue_100X y Fles oftype Al supportedfomats y Cancel Image Information Preview More than one image can be opened within ToupView simultaneously by a with Ctrl left mouse button method and b Shift left mouse button method to highlight the files to be opened When opening an image ToupView places it into a new image window It then becomes the active image Note ToupView maintains at the File gt Recent Files submenu a list of the last 4 Can 13 Help Manual be 1 8 opened files Any of these files can be accessed by simply clicking on file name If no files are listed in the File gt Recent Files submenu the Open Imageeee command must be used to open the file Also the View gt Browse can be used to view images in an Icon format under any selected directory Brief information is given in the View gt Browse menu File name From this list box select the name of the file want to open Either the type of the file name with its entire path
123. light When this menu is checked clicking the row in the Measurement Sheet will Highlight the corresponding Measurement Object over the Background Layer Clicking the Object over the video image will Highlight the corresponding row in the Measurement Sheet 8 2 6 Settingseee 39 Help Manual Measurement ES General Define the columns of the measurement sheet Length Unit Y mia Up W Name Angle Unit Calar E Iv Dan v Area Object Length Default Angle y Start Y End Distance 1 To modify the Measurement Sheet s item order click the item to highlight it the Up or Down button will be enabled If the item is in the third position the Up button will be disabled if the item is in the last row the Down button will be disabled Click the Up or Down button to modify the item order Note The item Index and Name are always in grayed states this means these two items cannot be modified Both their hide show states and their positions 2 Checking Unchecking the item will show hide the item in the Measurement Sheet 3 Clicking Default will return to the ToupView s default settings 4 Item 1 Index and item 2 Name cannot be changed 8 3 Sort 8 3 1 Sort gt Sort by Names This command is for the Browse window only Sort the image files in order of names in the Browse window 8 3 2 Sort gt Sort by Type This command is for the Browse wind
124. lity 75 Save these settings as defaults Cancel Reset all Determine whether the current image will be saved in multiple Appended pages pages style or not Compressions Specifies a method for compressing the composite image data Image quality Save these setting as defaults For Help Manual For saving a 32 bit TIFF file one can specify that the file be saved with predictor compression but have no option to use JPEG compression Predictor compression offers improved compression by rearranging floating point values and works with both LZW and ZIP compression If choosing Compressions as JPEG the Image quality can be adjusted by the slider bar The values range between 0 and 100 Default value 75 When saving a file the current settings will be saved as defaults for the next file save operation Compuserve GIF gif PCX pcx Targa tga JBIG jbg ToupView File Type tft There is no Option Note a Detailed information of the above academic terminologies can be found in books about image processing and image compression or internet b The file saved directory can be restored for future application To keep the directory unchanged when ToupView is started again choose Options gt Preferenceseee command click Misc page and check Restore the current directory when startup under the Privacy item Preferences Quick Save File Print Cursor Captur
125. llipse will be listed as Axis column when 122 Help Manual selecting None no column shows up Click Export and then the statistical result together with the image of Segmentation amp Count will be exported to an Excel sheet for further analysis and processing 12 5 Image Stackingeee The image stacking method is a form of speckle imaging commonly used for obtaining high quality images from a number of short exposures with varying image shifts It has been used in astronomy for several decades and is the basis for the image stabilization feature on some cameras The method involves calculation of the differential shifts of the images This is easily accomplished in astronomical images since they can be aligned with the stars Once the images have aligned they are averaged together It is a basic principle of statistics that variation in a sample can be reduced by averaging together the individual values In fact when using an average the signal to noise ratio should be increased by a factor of the square root of the number of images Image stacking can also be used for biological microscope image and florescent microscope image to increase the image SNR and dynamical ranges this is why we introduce image stacking into ToupView ToupView Image Stacking introduced advanced image matching techniques for rotated shifted and scaled images The user can record a short video to get a high quality stacked image at ease without consid
126. n is a Exposure Time 10 000ms g reference region for judging if the image brightness reach to the Exposure Target value _Defauts Drag or resize the Exposure ROI to the dark area will increase the video brightness and drag it to Help Manual the brighter area will decrease the video brightness a 2 Uncheck the Auto Exposure box to switch the Rectangle for Auto Exposure Auto Exposure mode to the Manual Exposure The Exposure Target slider will be disabled in this mode 3 Tune the microscope light source to a bright state and then drag the Exposure Time Slider left or right until the image brightness is normal 4 If and only if the microscope light intensity is too low to meet the Exposure Time up limit drag the Gain slide right until the video brightness is normal 5 The exact Exposure Time can also be entered by clicking the edit box at the right of the Exposure Time This will bring up a dialog called Exposure Time You can type the number in the field to set the exact Exposure Time value 5 4 White Balance group 1 Click the White Balance bar to expand the A White Balance group and a red rectangular E marked with White Balance will be overlaid on the i video window Move the ROI Marked by a red rectangle to a pure white or gray object and click White Balance to establish the white balance for future video 2 Drag or resize the red rectangle to a pure White Balance o Defaults white or
127. n item and the Deleteeee button will be enabled Click Deleteeee to delete the selected item 2 Highlight the Magnification item click the Up or Down button to modify the Magnification list order The Up or Down button will be disabled when the highlight item is at the first or last position 3 Click Clear Alleee to delete all of the Magnification items If one wishes to use the Magnification late a backup should be made first 4 Click Exporteee to back up the Magnification in a safe media The file extension is magn 5 After the new installation is finished the Magnification can be import by choosing the Options gt Magnificationeee command and click Importeee to load the previously saved magn file 6 If everything is ok press OK to end the Magnification management dialog 15 4 Calibrateeee de The detailed calibration steps are as follows 1 Run ToupView 2 Connect the camera to the computer and microscope 3 Start the camera Here it is VCMOSO0350KPA 167 Help Manual 4 Switch the microscope object to 10X and put the TS M1 micrometer in the middle of the microscope field and try to find the ruler clearly Set the Unit to Pixel and the video Resolution to the maximum one 640X480 for UCMOSOO350KPA and Zoom ratio to 100 see Pel _ Ub E Calibration Slide TS ha 106011 1Div 0 01mm x Ruler 100 0 01mm 1 mm ATou pTek 5 Choosing the Options gt Calibrateeee command or cli
128. n will close the video window directly 2 For MDI video window click x on 10 x to close the video window 3 Choose the Windows gt Close All command to close the video window Help Manual 3 Image window GUI 3 1 Image window GUI Fle Edt vew Setup Capture Image Process Layer Measurements Phign Options Window Help 3d B mam im 1 25 oN wm OAM 82 74 1 0000 Of EX SEMA w Background i Layel wW Layer2 w w Layer3 M Pixel nanometer rm E miaon um milimeter mm centimeter cm meter m Copy Full Path inch in Browse Containing Folder Customize Open Containing Folder Angle Unit Size on disk 207 MB 2 174 976 bytes Created Friday September 23 2011 1 03 43 PM Modified Friday September 23 2011 1 03 43 PM Accessed Friday September 23 2011 1 03 43 PM Attributes I Readonly I Hidden Advanced A ToupView B The opened file right mouse button context menu on status bar C The opened file name and directory D Double click bring up opened file Properties dialog E Current image width and height F Double click bring up Scale Image dialog G Image Zoom ratio double clicking will zoom the image to 100 H Zoom ratio right mouse button context menu I Currently selected Unit J Double click bring up Measurement dialog K Image BPP amp Resolution L Double click bring up Resolution Setting dialog M Unit right mouse
129. nd selecting its Option button in the Filter window the two methods ultimately do the same thing Because these kernel files are essential to the operation of these filtering options they must not be deleted or renamed Check to modify the kernel for a selected Filter type either Convolution Filter type l l or Morphological filters Edite e e Check to modify the selected filter kernel using the Edit Kernel dialog This combobox contains the name of the selected kernel file If one wants to save the modified kernel file to the same Names eo file leave it as it is If one wants to save the file to a new location enter the new filename here Click the spin buttons or enter the number to change the Kernel size of the kernel Either direction may take into account Size one to nine pixels As one modifies the Kernel Size the shape of the kernel representation changes accordingly In LE AECI the center of the dialog there are white boxes containing coefficients that will be multiplied with each pixel that will be taken into account by the filter kernel One can change any coefficient by clicking on it and adjust it as desired Click this button to fill every element of the kernel with a particular value The Fill kernel dialog appears One may l enter a value between O and 10 Using the Fill button is useful for setting all coefficients to the same value One may then change the coefficients that require a different value The
130. ne drawn the actual outline of the segmented object 2 Circle The actual outline fitting into a perfect circle users can get fit Radius of each object in the Count Result dialog 3 Ellipse The actual outline fitting into a ellipse users can get fit Axis of each object in the Count Result dialog When checking With Holes function the segmented object s Outline will be marked with Color Meanwhile the holes are also marked with Color and its parameters will be output in the image Hole is defined as the target grayscale or the color close to the background pixels The left figure below is the segmentation result without checking With Holes the right below is on the contrary With Holes option is usually used to get hole parameters Style Including 3 options 1 Outline objects are marked by outline the color can be user defined 2 Filled the segmented objects will be filled with custom colors 3 None no marked outline for the segmented object Color Custom colors used to mark the Style 104 Help Manual Method Including 3 options 1 XOR with the current pixel s XOR color to mark the current object segmentation 2 True marked with the specified Color 3 False no mark for the objects Note If users choose False in the Label group box then regardless of the type of Method there is no display for Index Area or Perimeter But the None Circle and Ellipse will displayed on the segmented image That s why ToupView call
131. new Dye List feature in ToupView allows you to select from a list of dyes or add your custom dyes to the list The list of dyes toupview dye is stored under C Program Files ToupTek ToupView directory Edit Dye List EN Name 1 5 IAEDANS Extraction Wavelength 336 a gt 4 lt Emission Wavelength 482 Color Red 000 Green 214 Blue 255 New Delete Save Reset Reload Al When you choose Options gt Edit Dye Listeee command you will see the Edit Dye List 170 Help Manual dialog box The list includes any dye definitions found in the current location When this feature is used to prompt for a specific dye the list indicates the currently selected dye or you may select a dye from the drop down list Name This list includes all dye definitions found in the current dye file You may select a different dye from the drop down list and all the remaining controls will be updated to show the characteristics of the new dye Emissions Wavelength This field displays the emissions wavelength for the dye you have chosen You can adjust he emissions wavelength by entering a new value The default color will change in response to changes in the emissions wavelength Excitation wavelength The default wavelength for your dye is displayed here You can adjust the wavelength by entering a new value Color Use the slider to adjust the emissions wavelength for your dye which also sets the default display color Yo
132. ng the Color Value slider over to the far right is a choice of white which results in a non tinted gray image The elements of the Select Color dialog box are Color Bar The Color Bar shows a rainbow displaying the Colors underneath the appropriate point of the Color Value slider Note 128 Help Manual If Color Value Slider is the selected highlighted selected control then you can also control the slider with keyboard strokes Use the left right arrow lt gt or lt gt keys to move the slider incrementally Press the lt Page Up gt and lt Page Down gt to move the slider in chunk sections along the Color Bar Use the lt Home gt and lt End gt keys to move the slider to the beginning and end of the Color Bar Selected This color block displays the new Color selected by the Color Value slider bar or the Color Palette Selected Color The Selected Color text field displays the corresponding numeric Color value of the Selected color block described above The Selected Color value is expressed as an HSV color with the red value first followed by the green and blue values If the color corresponds to a dye color the dye name will be displayed If the dye color corresponds to a color palette color the name of the color will also be displayed Current The Current color block displays the starting Color of the channel Color Palette The Color Palette proves an alternate method of selecting a Color
133. not checked choosing the Edit gt Select All command will select all of the Objects on the Current 34 Help Manual Layer shortcut Ctrl A 7 8 3 Select all files in the browse window When the Browse window is active choosing the Edit gt Select All command will select all of the files in the Browse window under the specified directory in the Folders Sidebar See Edit gt Invert Selection for select all files method 7 9 Select None Ctri D Deselect any selected area on the image or the Measurement Objects on a Layer 1 When the Current Layer is the Background Layer and an image area is selected the Select None menu will be enabled Choosing the Edit gt Select None command will delete the dotted rectangle representing the selected area 2 When the Current Layer is not the Background Layer and the Measurement Objects are selected the Edit gt Select None command will be enabled Choosing the Edit gt Select None command will deselect all of the selected Measurement Objects Note See Edit gt Image Select Edit gt Select All and Measurements gt Object Select M to understand how to perform select operations 7 10 Inverse Selection This command is for Browse window only When organizing files one can invert a selection in order to select all of the other files that were not previously selected with the Edit gt Invert Selection command When no files were selected choosing the Edit gt Invert Selection command will select
134. o parallel lines will be labeled and numbered El Appearance Line Width 3 Line Color E coor Line Style E Coordinates Start 88 Startl Y 61 End1 x 211 End1 Y 133 Start2 X 25 Start2 Y 77 End2 x 248 End2 Y 208 Calculation Length 50272 90 91227 Slope 0 59 Angle 149 66 Distance 16096 83 gt Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 6 Vertical FUE 145 Help Manual 14 6 1 Vertical gt Four Points 1 Move mouse and click its left button to mark the 1st point 2 Move mouse and click its left button to mark the 2nd point A line the 1 line connect these two point be overlaid on the image 3 Move mouse and click its left button to mark the 3rd point 4 Move mouse again one will find the 4th point is always restricted to perpendicular to the line of point 1 and point 2 Click to mark the 4th point Two vertical lines will be labeled and numbered Note a To check or modify the parameters of the selec
135. ocess ane Ra Stitching finished image In the stitched image there are some black areas These are happened when there are translations between the images in both directions 12 2 2 Image Stitch When the image window is active choosing the Process gt Stitch command will invoke the image Stitch dialog x Available Images Selected Images Add Add all gt gt Clear Direction Horizontal z Run Background v Available Images The images opened by ToupView 90 Help Manual Selected Images The images selected for stitching Add gt gt When an image or images is or are selected and highlighted in the Available Images window the Add gt gt will be enable Click Add gt gt will add it or them into the Selected Images window Add All gt gt Click Add All gt gt will add all of the Available Images into the A nee Remove When an image or images is or are selected and highlighted in the Selected Images window the Remove button will be enabled click Remove will remove it or them from the Selected Images window Clear Used to clear all of the images in the Selected Images window When no image in the Selected Images window it will be Direction The image stitching direction Horizontal or Vertical The default is Horizontal Run The stitching run method Background or Foreground method The default is Background Click Ok to begin image stitching Only the Selected Images are more
136. oge Layer LASA ogiera o been AA eaea AAA ADA EAS ADA Gerad 138 13 5 Layer menu and layer sidebar page context menu 139 SO MEWSE acera rene cera 139 Los 7 Remes PO a aa e Boana e 140 LAO CUEESTESES raras 140 LA MO CER E IED AD IA E AA E 140 ISA A A 140 Ll ADO E EO TAG iii AECA AE AAA AAA 140 13 12 Export to Microsoft ExCeL 2444 6 626 66 68 6668666866686668666868 140 14 MessutementSs b21 ee a ee a a ee ds da ds E 141 14 1 Object Select EEE 141 Poe angle Warrior aa aaa daa 142 14 3 Point Oo a E E E E E E 143 Contents A A et agcgn a tek agaas sg ge ages me GL ae age a cm me a aoe ge Gh mn me ge age a ge gs ae me Gham eg 143 tidy Line gt Arbiteaey Dene Murieron ens 143 14 4 2 Line gt Horizontal Line _ ee ee er ere eee 144 14 4 3 Line gt Vertical Line RETER ELA EAEE A A AEA 144 liso EM E E E EE E hse E EEE ee et ee E aE ek eee 145 14 6 Vertical B ro a oe ee ee eee eee eee 145 1151 Vertical our Points M cpcccecve ners ese sw ewenewexens 146 Ia 65 Vertical gt thres Points Mh casareuacecassiadeeacaaeanaes 146 14 7 Rectangle eee re era a mene een ee meee cas ata ee 147 14 8 RoundRect Oo 6665656 550666 55 56 9656 066656505056 5665658 147 14 9 Ellipse ee ee ee ee ee eee A eee ee oer ee ee 148 A MII II IN 149 14 10 1 Circle gt Center Radius MEA 149 14 10 2 Circle gt Two Points 149 14 10 3 Circle gt Three Points O E S 150 ML Davari MD esse wey ar rr e e A A A AA we 150 14 12 Two Circles A ee eee ee
137. omorphology on geodesy and each image pixel gray value indicates the altitude boundaries of each local minimum value and its impact area called catchment basins and the formation of watershed catchment basins So the formation of the watershed concept can be illustrated by simulating the immersion process In each local minimum value of the surface piercing a hole then the entire model slowly immersed in water with the immersion of the deepening impact of the domain of each local minima slowly expands outward in two catchments basin at the confluence build dams that form the watershed Watershed is suitable for relatively simple background target object or target object with a relatively large difference between the background images instead of the more complex application Choose Process gt Segmentation amp Count gt Watershed W there will pop up the following dialog Note Segmentation amp Count function only works for 24 bits otherwise all the sub menu items will be disabled Users can change the Color Bits to 24 bits by choosing Image gt Mode gt Color Quantize 103 Approximation With Holes Outline Help Manual Count Options Ey Approximation None v With holes Outline Style Outline v Color a Default Y Cancel Label Method True v Color Type Index v Y Area px Minimum Maximum v Perimeter px Minimum Maximum Approximation including three options 1 No
138. ontinus the video stream process Thus it will take more time to capture a still image 10 2 Time lapse Auto Capture This function can capture a sequence of pictures with the same time interval you can precisely set the time interval 2 to 3600 seconds and the total number of images LA Choosing the Capture gt Time lapse Auto Capture eee command will bring up Time lapse Auto Capture dialog shown below Directory The file Directory can be select by clickin the Browse button File The File name including Name of Format File Prefix File type It can be a combination of prefix time and type and is shown in the Sample Time Slot Time slot Second 2 3600S is a time segment to capture an image Toutle Images Checking Total Images will enable its edit box You can enter the Total Images 1 9999 to be captured ToupView will stop the Time lapse capture process automatically when the Total Images are reached 60 Help Manual If Total Images is unchecked ToupView will capture the images continuously until you choose the Capture gt Stop Time lapse Auto capture command again to stop the Time lapse capture Time lapse Auto Capture t x P C Users User Documents ToupView El Cancel m File Name Format yyyy mm dd HH MM SS v File Prefix rr File type png v Option Sample TT 2012 04 21 16 51 42 png Time slot second f6 44 V Total Images 100 Click OK to be
139. opy Copy the highlighted operation in the Undo Redo Sidebar to a new image window User can also drag the selected operation in the Undo Redo Sidebar to the window area to create a new image window CC Remove the highlighted operations from the Undo Redo list This button will be enabled only when the operation s is are selected the opened image the first operation cannot be removed CD Indicating the Current operation displayed in the image window CE Operation Index CF Operation name DA Layer Sidebar DB Make a New layer DC Remove a layer DD Set as the Current layer DE Show Hide a layer DF Rename a layer DG Visibility control of the layer items DH The Current active layer for operations DI The layer Name The image layer is always named as Background EA Measurement Sidebar EB The Appearance of the highlighted Object on the Current layer you can edit the Appearance by clicking its item and editing it EC The Calculation of the highlighted item on the Current layer ED The Coordinate of the highlighted item on the Current layer you can edit the Coordinate by clicking the item 8 6 2 Sidebar gt Camera The Camera Sidebar is mainly used for the control of Toupcam camera it includes 11 groups Every group can be expanded by clicking the group name or the arrow button at the right of the group name Checking the Sidebar gt Camera menu will activate show it on the frame Please check Camera Sid
140. orizontal Reverses the image in the application area so that the top right corner of the original image is now the top left and the top left corner of the original image is now the top right corner 11 3 6 Flip Vertical Reverses the image in the application area so that the top right corner of the original image is now the bottom right corner and the top left corner of the original image is now the bottom left corner 11 4 Crop Shift C Choose the Image gt Crop command to remove the portions of an image that does not want so that the focus is on the part of the image that is left This document instructs users on how to Crop an image in ToupView Crop Demo 76 Help Manual 1 Choose the Open Image e command to open an image to Crop 2 Choose the Edit gt Image Select command or click the Image Select button amp on the Toolbar the cursor will change to a small cross 3 Move the cursor over the image to the desired location click the mouse button and hold it down to mark the first position 4 Drag the mouse over the part of the image to be kept a dotted rectangle appears around the selection and release the left mouse button 5 Optional 1 To move the rectangle a Move the mouse over the selected area and when it becomes a move cursor click and hold the left mouse button b Drag the selected area to the desired position 6 Optional 2 To change the size of the rectangle a Put the mouse cursor on one of
141. ow only Sort the image files in order of type in the Browse window 8 3 3 Sort gt Sort by Size This command is for the Browse window only 40 Help Manual Sort the image files in order of size in the Browse window 8 3 4 Sort gt Sort by Width This command is for the Browse window only Sort the image files in order of image width in the Browse window 8 3 5 Sort gt Sort by Height This command is for the Browse window only Sort the image files in order of image height in the Browse window 8 3 6 Sort gt Ascending This command is for the Browse window only Sort the image files in order of the Ascending mode i e 1 2 3 and 4 in the Browse window 8 3 7 Sort gt Descending This command is for the Browse window only Sort the image files in order of the Descending mode i e 4 3 2 and 1 in the Browse window The Sort settings are saved until they are changed For example if you sort images in the Browse window according to the Type the images will remain sorted according to Type until the Sort settings are changed 8 4 Icon 8 4 1 Icon gt Large Icons This command is for the Browse window only The Thumbnail mode displays small preview images you can select different view modes in the Browse window Choosing the Icons gt Large Icons will display the image files in Large Icon mode in the Browse window 8 4 2 Icon gt Small Icons This command is for the Browse window only 41 Help Manual
142. pe v Area px Minimum Maximum Y Perimeter px Minimum Example of HSV Histogram is as follows 1 0pen an image and then choose Process gt Segmentation Counting gt HSV Histogram menu a Histogram dialog shows as below When the mouse moves in the image area the cursor will be displayed as Color Picker Cursor Histogram Cancel OK Options vez v 0 213 E Hue v Y Show All te weg ns ER gt s q AS HSV 186 114 206 2 Move Color Picker Cursor to any pixel of the image shown as above HSV value of the current pixel will be displayed in the bottom Histogram window Here H is 186 Sis 114 Vis 206 showing the HSV value of the ideal segmentation area record the three values Move the mouse to the background area the HSV value of Color Picker Cursor is 214 87 228 In order to accurately segment out the target users can set HSV values of the segmented pixels in the initial interval and those of background out of the initial interval For example the HSV values can be set to the initial interval as follows H is set to the initial interval 60 200 113 Help Manual S is set to the initial interval 90 130 V is set to the initial interval 0 210 When setting completed the segmentation effect is shown as below 4 Move again the Color Picker Cursor to any other unshadded pixels then the HSV value shown in left bottom of his
143. pply 2 Clicking the Capture page and then checking Capture with Marker and Watermark or Capture with Measurement 3 Click Ok to end the setup or Apply to apply the setup or Cancel to cancel the setup After the above steps are finished if there are Marker Watermark or Measurement objects overlaid above the video choosing the Capture gt Capture Image or clicking on the Camera Sidebar will capture the image with Marker Watermark or Measurement objects overlaid on the image 15 1 7 Misc Page The Misc page is mainly used for the control of the ToupView UI It mainly includes 1 ToupView special file format warning information 2 Sidebars 3 UI Style 4 Language 5 Privacy settings et al 161 Quick Save File Print Rulers and Grids Cursor Capture E Print Rulers and Grids Cursor Capture E Help Manual Preferences Taf Show the splash window on startup Y Prevent screensaver when video preview capture la tft File Remind me when save file with measurement not in tft format i Sidebars Automatically activate the camera sidebar when starting the camera Automatically activate the measurement sidebar when measuring Automatically show the browse window when double clicking the folders Automatically activate the camera sidebar when startup I Style O Windows K un 8 8 8 E a Windows 7 Visual Stud
144. processes before sending the image to the device The File gt Printeee command also has facilities that let one adjust the size and position of the image on the printed page 6 15 Recent Files ToupView maintains 4 default most recently opened document files under the Recent Files menu Choosing one of these submenus will reopen that file 26 Help Manual immediately Preferences Ez Quick Save O Office 2007 Silver A Visual Studio 2005 File Office 2003 8 Office XP Print _ Visual Studio 97 Lal Language Take effect after restarting ToupView Rulers and Grids Default 8 English Cursor O French Francais German Deutsch Capture _ Polish Polski Russian Pycckuii Simplified Chinese 78114 PX O Traditional Chinese HEP X O Turkish T rk e amp Privacy The maximum number of Recent Files 4 Clear the Recent Files when exit the application Y Restore the current directory when startup Misc lt cone 1 The maximum number of Recent Files can be modified by choosing the Options gt Preferenceseee command and clicking the Misc page Here clicking the 4 default edit box will allow you to enter the number of the Recent Files submenu that you want The value ranges from 0 to 8 the default is 4 2 One can also check the Clear the Recent Files when exit the application to clear the Recent Files after exit ToupView application 6
145. rby Note If the background pixels of a single channel is within the initial interval no segmentation change will take place Only all three channels of the background pixels are within the initial intervals can the pixels be segmented The segmentation effect by readjusting G initial interval is shown as below 110 Help Manual 5 Repeat step 4 and readjust initial interval of each RGB channel to achieve perfect segmentation 12 4 5 HSV Histogrameee HSV Histogram segmentation is based on image histogram where image pixels between a specified histogram upper and lower range will be color processed The segmented area can be adjusted by mouse to drag two vertical lines in histogram or by inputting precise value in HSV upper and lower edit box directly and clicking Enter to make it effective The Color representing the segmentation area can be customized by system palette For a 24 bits true color image segmentation can be realized by H S V channels respectively Choose Process gt Segmentation amp Count gt HSV Histogrameee shows Histogram dialog each function is specified as below Histogram T E 79 E Green v Show All Left Value in the currently selected region of histogram segmentation corresponding to Left Vertical Line position in histogram Users can segment the pixels bigger than the Left 111 Seo Manuel hmmm value by inputting a value in this edit box or dragging the Le
146. riginal coordinates 0 0 9 7 Rotate Watermark 9 7 1 Rotate toeee If there is Watermark overlaid on the video this menu will be enabled Choosing the Setup gt Rotate Watermark gt Rotate toeee command will bring up a Rotate dialog where one can define an angle to Rotate the Video Watermark a specified angle around the video center 0 0 default value 55 Help Manual Rotate 9 7 2 Rotate to zero If the Video Watermark was rotated the Rotate to zero menu will be enabled Choosing this menu will rotate the Video Watermark to zero degree 9 8 Gray Calibrationeee This function can make the image brightness a desired value among various scenarios in a specified area achieving the continuity requirement of the observation The Gray Calibration steps are summarized as follows 1 Click the Exposure amp Gain group name on the Camera Sidebar to expand the Exposure amp Gain group uncheck the Auto Exposure box If it is checked Selected rectangle for Gray Calibration po q a El a E aay 5 Gray Calibraion ES Average Gray 201 0 Zs lt 2 Choose the Edit gt Image Select command or click the Image Select button on the toolbar to select a reference region and choose the Setup gt Gray Calibrationeee command A dialog called Gray Calibration will be brought up to display the current ROI Average Gray Now the brightness of the microscope can be adjusted until the Average Gray reaches to the de
147. s we make our first curve Raising the curve increases the overall brightness of the scene c Conversely clicking in the center and dragging downwards lowers the brightness producing an image that s darker overall d As well as clicking in the center we can also adjust the endpoints Clicking the top right point and dragging down limits the brightest part of the image reducing contrast e If we drag that top right point to the left rather than down we produce the opposite effect increasing the contrast of the image This is a very useful and controllable quick fix f By dragging the top and bottom points towards the center we create a stylized posterized effect that turns any photograph into more of a graphic object g Click once in the center of the RGB curve to pin that midpoint now drag just the top half of the curve to make this S shape and the result is to increase the overall contrast h You can add up to any control points to the curve locking those values To remove a control point 1 drag it off the graph or 2 click the right mouse button on that point You cannot delete the endpoints of the Curve 68 Help Manual Grid Check to overlay the grid on the Curve window Channel To adjust the color balance of the image check the channel R G or B po Ea from the Channel button Check the white button to select RGB channels at the
148. s CMYK ToupView uses RGB mode for display on screen Although RGB is a standard color mode the exact range of colors represented can vary depending on the application or display device The CMYK mode is based on the light absorbing quality of ink printed on papers As white light strikes translucent inks certain visible wavelengths are absorbed while others are reflected back to the eyes In theory pure cyan C magenta M and yellow Y pigments should combine to absorb all light and produce black For this reason these colors are called subtractive colors Because all printing inks contain some impurities these three inks actually produce a muddy brown and must be combined with black K ink to produce a true black K is used instead of B to avoid confusion with blue Combining these inks to reproduce color is called four color process printing 72 Help Manual The subtractive CMY and additive RGB colors are complementary colors MAA Based on the human perception of color the HSI model describes three fundamental characteristics of colors Hue is the color reflected from or transmitted through an object It is measured as a location on the standard color wheel expressed as a degree between 0 and 360 In common use Hue is identified by the name of the color such as red orange or green Saturation sometimes called chroma is the strength or purity of the color Saturation represents the amount of gray
149. same time which is located on the left of the R Red G Green and B Blue buttons Click the Curve with your left mouse button a spline curve straight line will setup the initial input output relation Click on the Curve will mark the Curve with a point The point number can be any as long as it can be inserted on the Curve Drag the marked point until the image looks satisfactory The point can be deleted by move the mouse on it and click the right mouse button on it Check the pencil button at the bottom of the dialog and use your left mouse button to draw a new arbitrary Curve in the Curve area Click the Reset button will reset the Curve to the initial straight line Apply current Curve map on the image 11 2 2 Auto Level The Image gt Adjustment gt Auto Level command moves the level s sliders automatically to set highlight and shadow It defines the lightest and darkest pixels in each color channel as white and black and then redistributes the pixels color values proportionately Since Auto Level adjusts each color channel individually it 69 Help Manual may remove or introduce color casts The Auto Level command moves the level s sliders automatically to set highlight and shadow It defines the lightest and darkest pixels in each color channel as white and black and then redistributes the pixels color values proportionately Since Auto Level adjusts each color channel individually it may remove or introduce color
150. se filter will fill gaps and enlarge protrusions to connect objects that are close together Tophat Check this filter to detect and emphasize points or grains that are brighter than the background There are 3 kernel sizes for this processing Click the radio button to change the kernel size to the value that most closely matches the size of the grains to detect Well Check this filter to detect and emphasize points or grains that are darker than the background There are 3 kernel sizes for this processing Click the radio button to change the kernel size to the value that most closely matches the size of the grains to detect Watershed Check this filter to separate objects that are touching The Watershed filter erodes objects until they disappear then dilates them again but will not allow them to touch The Watershed filter will not operate upon True Color images If one wants to separate objects in a True Color image he must first convert it to Gray Scale see Process Frame Image gt Gray Scale Thinning Check this filter to reduce an image to its skeleton When choosing this filter one must set the threshold that determines whether a pixel is part of the subject or part of the background see Options below The Thinning filter will not operate upon True Color images If one wants to thin a True Color image he must first convert it to Gray Scale Distance The distance filter is used to show the distances of pixels w
151. sired value Click OK to finish the calibration and return to the application area The desired value here is around is 200 56 Help Manual 9 9 Video Source Propertyeee This menu will be displayed and enabled only when the camera that supports DirectShow interface is started Choose the Setup gt Video Source Propertyeee command to invoke the Video Source Property dialog The Video Source Property includes several categories such as Color Exposure Extended Misc and ROI page et al These pages are used for the control of your camera x Color Exposure Extended Misc RO White Balance PN ag Ae Tint ss Fo 1367 Default Move the ROI marked by blinking rectangle to the blank part of the sample before you click the One Push button M Hue Saturation Brightness Hue T 0 Saturation _ _ _ _ _ _ _ 255 Default Brightness H 25 M Contrast Gamma Contrast 1 Default Gamma 100 Note Different camera may have different control contents If you have any problems with these controls try to contact your camera supplier to get the further technique help ToupView has no responsibility to help you about these controls 9 10 Video Stream Formateee This menu will be displayed and enabled only when the camera that supports DirectShow interface is started The ToupView Video Stream Format configuration dialog is shown b
152. sponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 153 Help Manual 14 16 Z Order of all Objects on the Current layer Objects on the Current layer 9 D Modify the selected Object s relative position to the higher place of the ove Up two on the Current layer Modify the selected Object s relative position to the lower place of the Move Down two on the Current layer 154 Help Manual 15 Options 15 1 Preferenceseee There are 7 pages for the Preference dialog They are Quick Save File Print Ruler and Grids Cursor Capture and Misc pages 15 1 1 Quick Save Page The File gt Quick Save menu or the Quick Save icon on the toolbar will be enabled when a a new image captured from the camera b an image window is created by choosing the File gt Paste as New File menu File gt Quick Save can save the file at quick way with no need to specify the file directory file name and file format All those are specified in Options gt Preferenceseee Quick Save property page Preferences x Directory Quick Save C Users feihong Documents T oupView File File Print Name Format nnnn sequence v Rulers and Grids
153. st pixel camera is installed command to create the video window 16 Help Manual Camera Sidebar Click the Camera Sidebar If it is not activated and the Camera List group to expand it if not expanded Click the camera name Here it is UCMOSO3100KPA to create the video window B Camera List a UCMOSOS100KPA Note a Only a single video can be opened at a time ToupView takes camera as a special video file if the camera video window is opened the File gt Open Video menu will be disabled and the video file cannot be opened anymore b If a video file is opened the camera cannot be started 6 5 Twain Select Device Twain is a cross platform interface for acquiring images captured by certain scanners digital cameras or frame grabbers The manufacturer of the Twain Device must provide a Source Manager and Twain Data Source to work with ToupView Select the active device for the Twain Acquireeee menu from all devices available in the device combobox which are enumerated by the application One must install the Twain Device hardware and its driver first See the documentations provided by the device manufacturer for the installation instructions Before begin to start the Twain Acquire at the x first time with ToupView choose the O T UCMOS14000KPA 1 2 32 32 UHCCDOO800KPA 1 2 32 32 UHCCDO1400KPA 1 2 32 32 UHCCDO2000KPA 1 2 32 32 a UHCCDO3100KPA 1 2 32 32 gt _Cancal UHCC
154. stogram Equalizaci n cera nta ia ia ata ta aa Ga ta 71 Contents 11 2 5 Brightnhess Contrast t t cross ar Aa 71 11 420 COLO ets gyra e EA A AA A AA A A A AA AAA 12 11 2 7 PIT 74 AA A RRA 74 Lily 2e OD Filter Color eeg sase eea e a a A a Se Be oh Se A a a iaa 74 Esto 10 Extract COLO rset oa 75 Lit de ASEO III ER SAA IR IS A ROTATES icia ioi ea i a a E a a aa a a eee ee EA a a ee ea ee a a i ee ee 10 Ll LL UU AIDA 75 theta hou Ee e e OR RON 75 dba DCi ds ados laa odo da dea do a dos le ee ee 75 A alo rary ee oa io aue dni aue feu es ane aa aue ar are are anie ures ane ures Ree Gre bie Gre eee Grent 76 Too A e Orizont A ES 76 11 326 Flip Verticales craneo da 4 76 11 4 Crop SUL aus pr pr aaa 76 las Image CALLS qa saaa aaa AA ADA ADA AA AS 17 LLO TRS DOG aN OS oaoaraa aea aa aei A A AR 78 Ll 7 Resolutions e e aa EA A AAA A RA AAA RRA 80 12 PROCESS AAPP See se araara arona es araar 81 12 1 Filterees SOLAS pee SI III ISI 81 12 1 1 Image Ente iaa da vow A A AA AA beer EA AA a 81 12 1 2 Edge BDRANCS 22d 84 alos Morphological seco jezao seca seso seso sea sea sed sea sede 85 A A Pe Oe Se a aa 88 T22 DELEG aaa AAA AAA a 89 A o Er AS 89 T2 2 2 Image STICCha aaan anana 0 A A A A A a EI a a aa aa ea aa A 90 12 23 Browse Window 2 ELTON at AAA AA 92 12 3 EDFeee Sale oea e ce cece ee E oae 93 12 3 1 Browse Window EDF neses eec s aeaa don eae a a a a Goa a a a a a G 93 2 3 2 Image Window EDE soe dse ase ai dse ir are ri dre rii die p
155. surement under this Magnification choose 10X in the above Magnification dropdown combobox first and then choose Unit in the Unit dropdown list o a which is just in the left of the Magnification s dropdown combobox User can also choose Unit through the Option gt Measurementseee menu a dialog called Measurement will be brought up Click Length Unit page to display the Length Unit page and and checking Unit in the Current item xl 3 General dl ensth Unit Pixel px System NA Angle Unit nanometer LJ nm System 1e 009 Measurement 1e 006 Object nillimeter nm System 1000 centimeter cn System 100 neter m System 1 inch in System 39 37 add oo Delete Now you can measure the objects with the selected Unit at ease 169 Help Manual 01 13um T o 10 The selected Resolution can be saved for the future image Measurement operations If the Measurements are performed on the video the objects and Resolution can be saved in the image with ToupView File Format tft for the future applications 11 The other microscope Magnification such as 4X 40X 100X can also be defined just as the above steps Thus when you switch the microscope objective lens you just need to select the Magnification lx I for example on the toolbar to perform the Measurement operations 15 5 Edit Dye Listeee The Dye List can be used for the Process gt Color Composite operation The
156. t Frame styles and adjust the Text positions 4 Enter the text and click the right mouse button to end the Text object 152 Help Manual x A gt gt Background Color 0 Line Color E Default Y E Default y ol Line Style Tet Position Font E Microsoft Sans Serif xi 243 2 293 style Normal y sue fio gl y ras y 301 Align center y Color A Default w cm Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 15 Polygon sz Choose the Measurements gt Polygon command Move mouse to mark the 1st point Move mouse to mark the 2nd point eee 1 Move mouse to mark the nth point Repeat step1 to n to draw the other Polygon 7 Click the right mouse button to end the Polygon command Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corre
157. t gt Invert command to invert the image and choose the Image gt Color Quantizeeee command to convert the image to 24 bits as in Fig 3 Choose the File gt Save Aseee command to save the image in 24 bit BMP format pogu ologo o Fig 1 Captured Micro Ruler Fig 2 Micro ruler after being binarized Image File C Wsers ZHAOLEI Desktop ynr bmp E _ Conce Transparent 50 a Tips Use Shift Up Down Left Right arrow key to move the watermark Use Shift Ctrl Left Right arrow key to rotate the watermark Fig 3 Inverted 24 bits image Fig 4 Video Watermark setup dialog 4 Choosing the Setup gt Video Watermarkeee command and a dialog called Video Watermark is brought up as shown in Fig 4 Click the button to locate the image saved in step 3 Use the defaults Transparent 50 If everything is ok click OK button The final Video Watermark is overlaid on the video window as shown in Fig 5 54 Help Manual Fig 5 Video Window with Video Watermark overlaid 9 6 Move Watermark 9 6 1 Move toe ee If there is Watermark overlaid on the video window this menu will be enabled Choosing the Setup gt Watermarkeee command will bring up a Move dialog Where one can enter the X and Y offset value in their fields for the desired pixel move distances The default X and Y offsets are 0 9 6 2 Move to zero If the Watermark was moved this menu will be enabled Choosing this menu will move the Video Watermark to its o
158. t in this segmentation particle 16 located in the upper middle of the image is not a single object but actually two separate one However they have been calculated as a single object Choosing Split Objects will correct this problem Object splitting with Split Objects is discussed below As is shown in the image below move the cursor onto target 16 Click the left mouse button and draw a straight line When pressing down the left mouse button the cursor should not touch the target the starting point of the split line should be off the target that is to be separated Move the cursor to the bottom right of the target the ending point of the split line should also be off the target that is to be separated Release the left mouse button when this operation is completed A split line is shown between the two adhesive objects as shown below 120 Help Manual After the left mouse button is released ToupView will re count the whole image The result of re counting is shown as below The previous 16 turns out to be 12 and 17 now Unchecking or choosing Split Objects menu again will hide the split line The final result of hiding the split line is shown as below The whole Split Objects process is completed when the split line is hidden 121 Help Manual 12 4 8 Count Result T e Count Result is a way to show count statistical result of the current image Count Result dialog includes Index Center Rad
159. t the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 13 Arc 151 Help Manual 1 Move mouse to mark the 1st point 2 Move mouse to mark the 2nd point 3 Move mouse to mark the 3rd point then an Arc with Label and its Length will be drawn connecting these three points Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 14 Text 1 Choose the Measurements gt Text command 2 Move mouse to mark the 1st point 3 Move mouse to mark the 2nd point a rectangle with dash line restrict the Text window size After the mouse button is released a dialog called Text will bring up for you to enter the Text and define the Tex
160. tate You can see through transparent areas of a Layer to the Layers below You add a Layer to position the image content on the Layer like sliding a sheet of acetate in a stack You can also hide show a layer to make content invisible visible Layer3 Layer2 Layerl You use layers to perform tasks such as perform the measurement on the image without polluting the image and save it for the future adjustment for the further analysis adding text to an image or adding vector graphic shapes 13 2 Organizing layers A new image has a single Layer called Background layer The number of additional layers you can add to an image is limited only by your computer s memory You work with layers in the Layers Sidebar The Layer Sidebar help you organize and manage layers just like the Layer menu 13 3 Layers for non destructive measurement and label Rather than edit image pixels directly you can label the image with text and measure the image and leave the underlying pixels unchanged Because of the non destructively to images you can later tweak or remove the label and the Measurement Objects 13 4 Layer Sidebar 138 Help Manual A Remove Current Show Hide Anie Rename H Y Background Ds New Y O Layerl Renove I 5 ayara Current ls New J Show Hide Remove N New Eno E Rename Current ag Sho
161. ted Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 6 2 Vertical gt Three Points 1 Move mouse and click its left button to mark the 1 point 2 Move mouse and click its left button to mark the 2nd point A line the 1 line connecting these two points will be overlaid on the image 3 Move mouse and click its left button to mark the 3rd point The 2 line will be overlaid on the image which is perpendicular to the 1 one Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated 146 Help Manual automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 1
162. ted for reference It varies depending on the computer s configuration Different hardware configurations may have different Actual Frame Rates 9 4 Video Overlayeee 9 4 1 Video Overlay Overlay Choose the Setup gt Video Overlayeee command and click the Overlay page to overlay Scale Magnification Date and Clarity Factor on the video window This command will invoke Overlay page as above The Position Font Size Font Weight of the Scale Magnification and Date and Clarity Factor can be defined 51 Help Manual together Their Colors can be defined separately Clicking the OK button and the Scale Magnification and Date and Clarity Factor will be overlaid on the Video window The Clarity Factor can tell if the sample is in good focused state The larger the Clarity Factor the better the sample focused Note To enable the Scale bar the Magnification must be defined and chosen first The Unit can be any unit except Pixel There are two methods to set the Unit they are a Choosing the Unit in the Unit dropdown combobox Maw 7 1 71 mon the tool bar which is just on the left of the Magnification dropdown list box b Choosing the Option gt Measurementeee command a dialog called Measurement will bring up click the Length Unit page and check the Unit in the Current to set the Unit 9 4 2 Video Overlay Markereee Choose the Video Overlay Markereee command to overlay Video Marker on the video window The Video
163. the first 0 5 of either extreme Choose the Options gt Auto Correctioneee menu to modify this default setting This 70 Help Manual ensures that white and black values are representative without being determined by extreme pixel values The Auto Correctioneee dialog is shown below Auto Range Auto Level Low i x High 0 005 y 0 005 m Auto Contrast Cancel Low High 0 005 0 005 The Auto Contrast command can improve the appearance of many photographic or continuous tone images It does not improve flat color images See Image gt Adjustment gt Auto Level for another auto operation 11 2 4 Histogram Equalization Histogram Equalization is a kind of histogram process The histogram can reflect the statistical information for the R G and B of the pixels of the original image The algorithm calculates each separately equalizes the R G and B of the points linearly and reassigns them 11 2 5 Brightness Contrasteee The Image gt Adjustment gt Brightness Contrasteee command offers simple adjustments to the tonal range of an image This command makes the same adjustment to every pixel in the image The Brightness Contrast command does not work with individual channels and is not recommended for high end output because it can result in the loss of details about the image Adjust Brightness Contrast x Brightness D o Tee aot ymin ees EES A M Preview Preview Check th
164. the handles that appear on the edges of the selected area b Click and hold the mouse button c Drag the box to the desired size Note Each of the handles that appear on the edges of the box sizes the box differently 7 To Crop the image choose the Image gt Crop command or press Shift C buttons Note If the selected area is on the video choosing the Image gt Crop command will capture the selected area and display it on a new image window 11 5 Image Scale Choose the Image gt Image Scaleeee command to change the image to a specified size This process actually changes spatial resolution by adding replicating or removing decimating pixels to achieve the specified dimensions 17 Help Manual Width and Height When choosing the Image Scale command the dialog displays the dimensions of the original image in pixels The Width and the Height can be set on the new image by adding or removing pixels If Constrain Proportions is checked the Width and Height will stay proportionate to each other If Constrain Proportions is unchecked the Width and the Height can set independently but this will distort the image Width Reset IV Constrain Proportions Cancel Scale method Bilinear x Reset Reset the image Width and Height to the original ones Constrain Proportions To maintain the current proportions of pixel Width and Height check Constrain Proportions This option automatically updates the Width as the Heig
165. to apply the selection ON View Properties E xj pery Device ID device sw 860BB310 5D01 11D0 UHCCDO 1400KPA TP120117094046CE772A46CCB944 Grab Retry Count Grab Frame Speed Grab Percent One can find if the device supports Still Image Capture or not by choosing the Setup gt View Propertyeee command If the Still Image Capture s Value is Y in the View Property dialog this means that the camera supports the Still Image Capture operation 59 Help Manual 10 Capture 10 1 Capture Image F8 During the video preview you can always choose the Capture gt Image Capture command to capture the video image After the image is captured the captured image will be the current active window The Capture gt Capture Image menu will be disabled If you wish to capture image again click the video window title to activate the video window and the Capture gt Capture Image menu will be enabled again Note a The Snap button D on the Camera Sidebar can continuously shoot the image even if the video window is not activated Click this button on the Camera Sidebar to capture image quickly b Only when the video window is active the Capture gt Capture Image menu will be enabled c If the Live and Snap resolutions are different ToupView need to switch the resolution from Live to Snap in the background to capture an image with Snap resolution After the Snap is finished ToupView will switch back to the Live resolution to c
166. to browse images under the specified directory in the Folders Sidebar 2 Click the Folders Sidebar to activate it and double clicking the listed directory in the Folders Sidebar will create the Browse window After creating the Browse window ToupView will display a Browse window that looks like windows explorer The child window on the left part of the Browse window called Folders Sidebar is used to locate the directory on the hard disk Images in the current directory are displayed in Large Icons or Smal Icons mode on the right side of the Browse window Their order can be set in Forward or Reverse format according to Sort by Name Type Size Width or Height et al 8 1 2 Browse window right mouse button context menu Clicking the right mouse button on the listed Icon in the Browse window will bring up a right mouse button context menu as shown below Delete File Delete h gt boni Sort by Name I gt ies Large Icons Sort by Type 2 Refresh FS Snall Icons Sort by Size Properties Sort by Width Sort by Height Forward Reverse These context menu functions are described in View gt Delete File View gt Sort gt Sort by Names View gt Sort gt Sort by Type View gt Sort gt Sort by Size View gt Sort gt Sort by Width View gt Sort gt Sort by Height 37 Help Manual View gt Sort gt Forward View gt Sort gt Reverse View gt Icon gt Large Icons View gt Icon gt Small Icons View gt Refresh for details
167. togram is 194 79 212 as below e an z a Histogram Ez m Cancel ok Options ee v e i i 0 2 210 E Value v Y Show All 5 ON 4 O E gt gt HSV 194 79 212 gt O d a 90 d Oo so Fe oe oe 1 It s easily found that here S channel defined 90 130 its lower limit is too bigl to get the pixel segmented Then initial interval for S channel can be adjusted from 90 130 to 60 130 to segment the pixels nearby Note If the background pixels of a single channel is within the initial interval no segmentation change will take place Only all three channels of the background pixels are within the initial intervals can the pixels be segmented The segmentation effect by readjusting S initial interval is shown as below 114 Help Manual e vere pr segmentation 12 4 6 Color Cubiceee Choose Process gt Segmentation amp Count gt Color Cube a dialog called Color will pop up as below Color Ea E A 3 3 v Cancel RGB OK Tolerance Options G 2 G E 2 E At this moment the Colorizing Pipette Default is checked by default Click the image will set the average color value in the masked area as Basic Value ToupView will segment the image by choosing the pixel within Basic Value Tolerance color range and mark it with current selected Color By clicking the target pixel repeatedly ToupView can continuously add new pixels within the Basic Value
168. tton to apply the auto threshold process to the image It uses an automatic threshold to make the image Binary 12 9 Pseudo Coloreee Note Image must be in Gray Scale Choose the Pseudo Color command to colorize the active monochromatic image This is used to highlight certain features in a gray scale image such as display all densities above a certain point in red or the imaging device recorded thermal 132 Help Manual information all temperatures below a certain point can be revealed in blue color Pseudo Color x Color Table When Pseudo Color a monochromatic image a special palette need to be build with which the monochromatic image is displayed Pseudo Coloring an image does not modify the pixels values in image bitmap it does not convert image to true color or palette It simply associates a Pseudo Color palette with the image that interprets the gray level values in the image as color Pseudo Colored images are very similar in structure to palette class images but they differ in a couple of important ways First the pixels values in a Pseudo Colored image actually represent continuous tone intensity information whereas a palette image s pixels carry no intensity significance Secondly a palette image includes a palette table that is actually part of the image file The colors used to map the gray values can be selected The buttons at ESNS each end of the color strips m ors will brin
169. u can also use the Color button E to select a specific color using a standard Windows color selection dialog The color will change in response to changes in the emissions wavelength Here you can change the color associated with the selected dye or define a custom color The color default is linked to the selection of a wavelength for your dye If you change the Emissions Wavelength the color displayed will be the standard color for that wavelength To set a custom color first you should set the wavelength and then edit the color Color ES nel Eee TE in f f EE O i E tee FF 4 tee FF Euu i rs HE Hue 126 Red 0 i i i i A A Sat 240 Green 214 Define Custom Colors gt gt Color Solid Lum 120 Blue 255 Cancel Add to Custom Colors New Clicking New will setup a new dye this will invoke a dialog called New Dye 171 Help Manual Input your New Dye name and click Ok to end the dialog and click Cancel to cancel the input This will return to the Edit Dye List again and the new name will be the default on in the Name list box Try to set the Emissions wavelength Excitation wavelength Color for your new dye New Dye ES New Dye 1 cra Save Click Save on Edit Dye List to save your dye selection Close Click the Close button to end the Edit Dye List dialog 15 6 Auto Correctioneee Set the low and high ranges for the Image gt Adjust gt Auto Level and Image gt A
170. und Background Brightness is the offset value that is used to add or Brightness subtract the background Default 93 Range 10 240 Default This will set all 3 values to the Default ones Clicking Next gt button will pop up an Auto Align dialog as follows Auto Align EM Auto Align 8 None O Shift Scale _ Shift Scale Rotate Description Select the proper Auto Align mode according to whether there is shift scale or rotate among the images to be processed The time consumption of EDF is related to the setting of the maximum angle The time will increase when the maximum angle increases and the relationship between them is non linear The default maximum angle is 1 0 None Considering no Shift Scale Rotate among the selected images in the fusion process Shift Scale Considering Shift Scale among the selected images in the fusion process Rotation is not condsidered in this option Shift Scale Rotate Considering Shift Scale Rotate among the selected images in the fusion process If this mode is checked The Maximum Angle slide bar will be enabled Maximum Angle The Maximum Angle is used to set the upper limit in Rotate alignment The Default is 1 degree Range 0 1 10 User can select the proper Auto Align mode according to whether there is shift scale or rotate among the images to be processed The time consumption of EDF is related to the setting of the Maximum Angle The time will increase when t
171. upper limit and the upper end of the scale will be white These two edit control values corresponding to the two vertical markers show the upper and lower limits of the intensity levels Defaults are o and 255 respectively The Reset button allows Reset the black and white levels to the high and 131 Help Manual low ends of the dynamic Range The Best Fit button automatically sets the intensity levels to the Best Fit Best Fit Best Fit instructs ToupView to optimize the brightness and contrast values for the particular image The Invert button reverses the color of the image e Update will refresh the display Range with the most current image ate j information 12 8 Binary ee Shift B Binary is a kind of gray level process If the gray of the pixel is greater than the given threshold the pixel s color will be changed into white Otherwise the pixel s color will be changed into black Although the process may lose some information it is an important step of other processes x 193 a The curve on the Binary dialog shows the gray distribution of the image The Line in the dialog indicates the threshold value Drag the line to change the threshold or change the value in the Line position edit box in the top left corner of the dialog to move the Line The Line position edit box shows the current position value of the Line Modify the value will move the Line at the same time Click the Best Fit bu
172. view Current File Current File location on your computer Width Current File image width Height Current File image height Pa 6 2 Open Videoe ee The File gt Open Videoeee menu will be enabled only when there is no video file opened in the ToupView frame or no camera start up 1 Choose the File gt Open Videoeee command to open an existing video file D open xl O E DATA hep Denavir E Organize y New folder a fl 3X Favorites uy y BE Desktop Je Downloads Recent Places gt FlickAnimation avi MZ Desktop A Libraries Documents al Music Pictures Ew Videos 2 Homegroup JA ZHAOLEI ME Computer Cu Network ES Control Panel xl File name FlickAnimation avi Si Video Files wmv asf avi ces 4 2 Select the name of the file you want to open If the file does not appear select the option for showing all files from the Files of Type combobox The video file type can be wmv asf or avi format 3 Click Open to open a video file this will create a video window and begin to start the video stream The video window will be associated a name called Video XXX XXX i e its title bar will display Video XXX XXX here XXX XXX is the video file name 15 Help Manual 4 Click Cancel to return to the application Note Only a single video can be opened at a time ToupView takes camera as an special video file
173. w Hide Current Rename an Ue A Make a New layer B Remove a layer C Set the Current layer D Show Hide a layer E Rename a layer F Auto hide the Layer Sidebar G Right mouse button context menu for the Background layer H Right mouse button context menu for the Current not checked layer I Right mouse button context menu for the Current checked layer J Right mouse button context menu in the blank area Note a Only one layer can be checked as Current Layer If a layer s Current is checked the Visible will be checked automatically b The Current and Background layers cannot be removed Only the non current and non background layers can be removed c All the Objects on the Current Layer can be selected edited or exported d The non current Layer can be Visible checked or invisible unchecked 13 5 Layer menu and layer sidebar page context menu The function of the Layer menu and Layer Sidebar right mouse button context menu are the same They are New Remove Current Show Hide Rename 13 6 Neweee Setup a new layer After the new layer is setup it will be added to the end of the Layer 139 Help Manual Sidebar s items and the Visible and Current will be checked 13 7 Removeeee Remove the non current Layer Note the Background and Current Layer cannot be removed 13 8 Currenteee Set the selected layer as the Current Layer User then can edit the Object on the Current Layer or
174. wn below Help Manual 12 3 2 Image Window EDF Similar to the Browse window EDF Image window EDF is also a very useful tool to generate a clear image by combining a sequence of previously captured or opened multi focus images Choosing the Process gt EDFeee command the following dialog will be brought up assume Ol jpg 02 jpg eee 15 jpg are already opened in ToupView Select Files ES Open Selected 01 jpg add 02 jpg 03 jpg 04 jpg Add all 05 jpg 06 jpg 07 jpg 08 jpg 09 jpg 10 jpg clear 11 jpg 12 jpg 13 jpg 14 jpg 15 jpg Clicking on the image file name in the Open combobox will highlight the image and then the Add gt gt button is enabled Click on the selected images will deselect them Click the Add gt gt button the highlighted images will be added into the Selected list box which will be fused later Clicking the Add all button will add all images in the Open combobox into the Selected list box If images in the Selected combobox are highlighted the Delete button will be enabled Click the Delete button the highlighted images in the Selected combobox will be removed Clicking the Clear button will remove all the images in the Selected list box including the unselected ones The button will be disabled if there is no image in the Selected combobox 99 Help Manual When the desired images are all selected If more than 2 images are added in the Selected List box the N
175. y Page Mosaic Images x ImaList Property Page Title mageMossic Footer by FH Selected Images ass Available Images an addres 2006 hm 37 large jpa Arrangement Addal gt gt ROWS 2 Row spacing 1 V Auto Remove Columns 3 Colspacing l System font Font Images opened with Clear Images wish to ToupView sea TPageSize Default size Width 1280 Height 1024 Use printer s size 6267 x 11692 Change IM Use 1 4 Size OK Cancel Apply Cancel ImgList page Available Images Images opened with ToupView Add gt gt Add the opened images to the Selected Images list view Add All gt gt Add all the opened images to the Selected Images list view Remove Select the images and remove them from the Selected Images list view Clear Remove all the images from the Selected Images list view Property page Title The title wanted Footer The footer wanted Arrangement The Mosaic Images distribution on the page PageSize The page size for the Mosaic Images If everything is set click OK to end the Mosaic Images operations and a new image window will be displayed and the final results should be 136 Help Manual image Mosaic 20065 hm 68 1arge pg 2006 hm 37 1arge pa 2006 hm 36 12rge jpg Pg 1 by FH 137 Help Manual 13 Layer 13 1 About layer ToupView Layer is like sheets of stacked ace
176. you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculated items will depend on the selected Object type The type could be the same or different 14 9 Ellipse o 1 Choose the Measurements gt Ellipse command 2 Move mouse to mark the 1st point 3 Move mouse to mark the 2nd point 4 If it is not superposing with the shape on the image choose the Measurements gt Object Select command to adjust the position to adjust the Ellipse in order to coincide with the image shape 148 Help Manual E Appearance Line W idth 2 Line Color BB ooo Line Style Fil False Fill Color E cOc0co 8 Coordinates i Start 209 Start 35 End 271 End Y 105 Calculation Center 40636 64 11852 35 Axes 5248 90 5926 18 Perimeter 70215 07 Area 97722085 25 Note a To check or modify the parameters of the selected Object just select a single Measurement Object and the Measurement Sidebar will be activated automatically Here you can edit the corresponding Object parameters to modify its properties b The Appearance Coordinates on Measurement Sidebar will be enabled only when a single Object is selected The Calculation will be effective for a single selected Object or multiple selected Objects Its calculate

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