GenTegra®-DNA Sample Kit Technical Evaluation Plan
Contents
1. GentTegra Technical Note GenTegra DNA Sample Kit Technical Evaluation Plan GenTegra DNA Overview This technical evaluation plan can be used as a guide for accelerated stability testing using GenTegra DNA tubes Kit Contents The GenTegra DNA Sample Kit contains the following items e Three GenTegra DNA 1 7 ml tubes Before You Begin 1 Download a copy of the GenTegra DNA user manual at www gentegra com 2 Select a DNA sample to use for evaluation e For best results quantitate the DNA sample and run on a gel to check quality before you apply to GenTegra Tubes e GenTegra DNA tubes can accommodate between 50 ng 25 ug of DNA in a volume of 20 250uL 3 Prepare four aliquots of your sample three applied to GenTegra DNA tubes and one stored frozen as a control in your typical tube 4 Choose an accelerated testing program depending the amount of time available for evaluation and need for long term stability data Programs Program 1 One week evaluation Aliquot 1 GenTegra tube one week at room temperature 25 C Aliquot 2 GenTegra tube one week at 56 C equivalent to 8 weeks at 25 C Aliquot 3 GenTegra tube one week at 76 C equivalent to 32 weeks at 25 C Aliquot 4 Frozen control one week at 20 C Program 2 Two week evaluation Aliquot 1 GenTegra tube two weeks at room temperature 25 C Aliquot 2 GenTegra tube two weeks at 56 C equivalent to 16 weeks at 25 C Aliquot 3 GenTegra t2. rol aliquot from the freezer and run alongside the samples recovered from GentTegra in each type of analysis 2 Quantitate your DNA and run on a gel to assess yield and integrity 3 Use recovered DNA for a downstream application of your choice to assess quality and purity For further information or support 844 540 4000 toll free 925 461 3081 phone Copyright 2014 GenTegra LLC All rights reserved Information in this document is subject to change without notice GenTegra orders gentegra com assumes no responsibility for any errors that may appear in this document support gentegra com FOR RESEARCH USE ONLY www gentegra com GenTegra products are for research use only GenTegra is a trademark of GenTegra LLC All other trademarks are the sole property of their respective owners G enlegra Document Number P037451 Rev B 4 2012 GenTegra e 1061 Serpentine Lane Suite B e Pleasanton CA 94566 2 GT20140105A
3. ube two weeks at 76 C equivalent to 64 weeks at 25 C Aliquot 4 Frozen control two weeks at 20 C Program 3 One month evaluation Aliquot 1 GenTegra tube one month at room temperature 25 C Aliquot 2 GenTegra tube one month at 56 C equivalent to 8 months at 25 C Aliquot 3 GenTegra tube one month at 76 C equivalent to 32 months at 25 C Aliquot 4 Frozen control one month at 20 C Technical Note GenTegra DNA Sample Kit Technical Evaluation Plan Accelerated Stability Formulas Accelerated stability formulas are based on temperature dependence of rate limiting oxidative damage GenTegra stores DNA samples at up to 76 C to demonstrate long term stability e 1 day at 37 C is equal to two days at 25 C e 1 day at 56 C is equal to eight days at 25 C e 61 day at 76 C is equal to 32 days at 25 C References e Nucleic Acids Research 2002 30 1354 1363 e Nature 1993 362 709 715 Technical Evaluation 1 Apply DNA to GenTegra tubes and dry according to the protocol described in the user manual 2 Store DNA at indicated temperatures for appropriate length of time Room temperature samples may be left on your bench top 3 Elevated temperature studies should be conducted using dry ovens set at the appropriate temperatures 4 Following the storage period recover DNA according to the protocol described in the user manual and use for downstream analysis Downstream Analysis 1 Remove your cont
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