Concentrating Pipette User`s Guide
Contents
1. 0 4 um Polycarbonate L Limited Exposure 0 1 um Polyethersulfone Track Etched PCTE NR Not Recommended Concentrating Pipette Tips Concentrating Pipette Tips U Unknown 596 Acetic acid R R 2596 Acetic acid L Acetic acid glacial NR NR Acetone NR NR Acetonitrile NR U 0 1 N Ammonium hydroxide R R Copyright 2012 InnovaPrep LLC www innovaprep com 12 R Recommended 0 4 um Polycarbonate BM a uM Concentrating Pipette Tips TackEtched PTE U Unknown Concentrating Pipette Tips Conc Ammonium hydroxide NR NR Amyl acetate NR NR Amyl alcohol NR NR Aniline NR NR Benzene NR NR Butyl acetate NR NR Butyl alcohol E L Carbon tetrachloride NR NR Chloroform NR NR Chromic acid NR NR Cresol NR NR Cyclohexanone NR NR Diacetone alcohol NR NR Dimethyl formamide NR NR Dimethylsulfoxide NR NR Ethers NR NR Ethyl acetate NR NR Ethyl Alcohol NR NR 5096 Ethyl alcohol L L 9596 Ethyl alcohol NR NR Ethylene dichloride NR NR Ethylene glycol R 1096 Formaldehyde R 3096 Formaldehyde L 5096 Formaldehyde NR NR Glycerin R Hexane U U 596 Hydrochloric acid L L 2596 Hydrochloric acid NR NR Conc Hydrochloric acid NR NR 396 Hydrogen peroxide L L 3096 Hydrogen peroxide NR NR Isopropyl alcohol L L Methyl acetate NR NR 50 Methyl alcohol NR NR 95 Methyl alcohol NR NR Methyl chloride NR NR Methyl ethyl ketone NR NR Methylene chlori2. and prepare the new can for use To remove a fluid can push down on the top of the can pulling it away from the instrument to un latch it When the instrument is new the fluid can valve will tend to stick in the Can Interface simply grab the can with your hand and press your thumb against the can latch to leverage it up and out of the Interface Port If a can is removed before it is completely empty a small amount of fluid approx 100 uL will tend to spray out this is normal The Can Interface Port is the number one place where contamination can enter the instrument s fluidics Never let dust or dirt enter the port and use good lab practices to prevent bacterial contamination Copyright 2012 InnovaPrep www innovaprep com 7 4 3 INSTALLING AND REMOVING CONCENTRATING PIPETTE TIPS Figure 4 1 shows how the CPT ports are aligned with the ports in the Fluidics Head Extraction Port Permeate Port Ball Detent FIGURE 4 1 TIP INTERFACE PORT DETAIL Raise the Fluidics Head all the way up The Two ports on the top of the CPT are different sizes the larger port is the permeate port facing the rear of the instrument The CPT can only be installed one way and should slide easily up into the head When the CPT is fully seated you should feel it click into place You can also verify that that the CPT is fully seated by looking through the clear fluidics manifold To remove the CPT grasp it with your hand then use your thumb to pre
3. be precisely adjusted using the Adv Options menu The volume of elution fluid dispensed during an extraction is controlled by how long the extraction valve is held open and how many times it is opened For volumes different than the preset small med large set the Valve open MS time and the Pulse count and then perform an extraction with a CPT installed collecting the extraction in a tared sample vial Weigh the sample vial to determine the final extraction volume Adjust the Valve open MS time up or down as required to attain the desired volume Large volume extractions 2250 uL are usually more efficient if they are split up into several pulses rather than one long one Remember that there is usually a tradeoff between extraction volume and recovery efficiency Smaller extraction volumes will almost always produce lower recovery efficiencies than larger extraction volumes Multiple extraction cycles can be combined into a single final sample Sometimes this produces the highest total efficiency for a given final volume 7 3 CONCENTRATING A SAMPLE Once the instrument is set up concentration cycles are very easy to perform and repeat Be sure you followed the instructions in Section 6 to install the instrument have the elution fluid can installed and primed and have performed test extractions to confirm that the extraction is setup correctly To concentrate a sample install a CPT place your sample container on the sample platform then
4. bulk of the fluidic components in the instrument The Head can be raised and lowered to position the Concentrating Pipette Tip in the sample container LED lights in the head will glow blue when the instrument is on red when the instrument is concentrating and green when the instrument is extracting e Concentrating Pipette Tip CPT This is the only part that will come in contact with the sample e Can Latch This holds the fluid can in place e Fluid Can This easily replaceable can contains the elution fluid or decontamination fluid Copyright 2012 InnovaPrep www innovaprep com 6 e Can Interface Port This spring loaded port keeps the proper pressure on the fluid can valve it also contains a check valve which keeps the system pressurized when changing cans e Control Panel The LCD display is navigated by the up down and enter keys e Sample Platform This is where the sample container is placed e Permeate Port This quick connect fitting is where the filtered fluid Permeate exits the instrument This port is valved to prevent fluid from being pumped out without a permeate line attached e 24VDC Jack Inlet for the included power supply e USB Port A standard Mini B USB cable can be connected to the instrument from a computer to download software updates e Power Switch The up position turns the instrument the down position turns the instrument off e Arm Tension Knob Use this knob to adjust how tightly the head
5. lower the fluidics head so that the CPT is at the bottom of the sample container Go to the Main menu and press Start run The vacuum pump will immediately start to draw the sample through the CPT After several seconds you will see fluid flowing from the permeate line into your waste container Once all of the fluid in the sample container has been drawn into the CPT the flow sensor in the fluidics head will detect that the flow has stopped and the instrument will conclude the run automatically The instrument will display the Run complete screen show the processing time and the option to extract or return to the main menu Raise the fluidics head out of the sample container and hold the desired final sample container under the CPT Select Extract on the screen and press enter the concentrated sample will be dispensed from the CPT into the sample vial Wait until the progress bar on the screen has filled up before removing the sample vial Additional extractions may be performed by selecting Extract Again 8 TIPS AND SUGGESTIONS This section contains some dos and don ts tips tricks and important notes to remember while using the Concentrating Pipette Copyright 2012 InnovaPrep LLC www innovaprep com 11 e f the instrument has been idle with an elution fluid can installed for a period of time the quality of the foam may be reduced due to off gassing of CO in the fluid lines If the instrument sits overnight with an elution fluid can install
6. the beeps that occur at the end of concentration cycles and extractions The four options are low med high and off Default low Power LED turns the blue LED light in the Fluidics head on and off Default on Run LED turns the flashing red LED light in the Fluidics head on and off Default on Extract LED turns the flashing green LED light in the Fluidics head on and off Default on OPTIONS The Adv Options menu is used to set custom elution settings it has four main settings Valve open MS Valve off sec Pulse count and Flow buffer The settings control specific timing of the extraction valve in order to precisely control extraction volume over a wide range Values displayed on this screen coincide with the Ext size small med or large selected in the Options menu Manually changing these valve settings will override the Ext size setting Valve open MS controls the length of time that the extraction valve is open per pulse in milliseconds The minimum setting is 25 ms Increasing the valve is open time will increase the elution volume Default 75 Max 255 Valve off sec controls the timing that the extraction valve is closed between each pulse in seconds If the pulse count is set to 1 then this setting is irrelevant Default 0 2 Min 0 1 Max 20 0 Pulse count is the number of cycles that the extraction valve will open and close Multiple pulses are usually used when larger 2200 pL final volumes are desired For exampl
7. ONCENTRATING PIPETTE 8 NAVIGATING THE MENU SYSTED ccccssssssccccccsssssccccccessesccccacessceecccessesecceccensesececacenseessceessssesceeeees 8 5 1 THEMAIN MENU a bios pie Cic vv cer po Dor ku 8 5 2 THE OPTIONS MENU ssecccssecccnsscccnseccencecceesecceeceecnsesceeecsecusceseueceseuseesenssetenseeceessseeestecaeessensetsenees 9 INSTALLING THE INSTRUIMENID sccsclecacstacscescccacsasncacoacasersceccasstoagecaasasncsosaccsevecsscasssoagecencasssvouecsasesenuese 10 2 ec coe caay 10 7 1 THE DECONTAMINATION CYCLE c cssecccesscecssscccessceeesecceeseeceusecceuseeceuseeeenceeceeceseuecseceeceeeeeceeueeeesaeeeeeas 10 7 2 SETTING UP THE EXTRACTIONIz eere e red c re d wee ode Ye e E Due debate rao e ipaa dx Yeh 11 7 3 CONCENTRATING A SAMPLE TIPS AND SUGGESTIONS sccsccicsscccdicsssuccssassevccencascsoccucccstsnsvencoacsescoseceschsteucecssensvescoccsesessacoucbereeccesssnsees 11 CHEMICAL COMPATIBILITY GUIDE ssssssssssseccceneesesssssssscscccesesssssesssscsssssesseeseeseesssssssssccsseeseeeenes 12 Copyright 2012 InnovaPrep www innovaprep com 2 1 PRODUCT OVERVIEW This section contains a product description of the InnovaPrep Concentrating Pipette and a brief look at its applications 1 1 PRODUCT DESCRIPTION The InnovaPrep Concentrating Pipette shown in Figure 1 1 quickly and eff
8. The Macro to Micro Interface Concentrating Pipette User s Guide Copyright 2012 InnovaPrep www innovaprep com 1 Table of Contents dieis tleio dian 3 1 3 PRODUCT DESCRIPTION sic oso ao oie we vv oou se apa o ERR E UU 3 1 2 APPLICATIONS CONCENTRATING PIPETTE METHOD OF OPERATION sscccccsssssseecccccsscseccccccnsecesccccsssceeccessecceceseees 3 2 1 CONCENTRATION PROCESS iis csnnscdsdderconvsntcstedccesanvavesvedaddacievnenevcs reos deen Co eee E Deka rk pu EE ve pe Putri 3 2 2 WETFOAM ELUTION m m 4 23 FOAMIGENERATION ssacenecaxscenensdsotscode des clendhs curn Fox Ecke de e Dec ex de de s Qo Feb e Pr dva 5 2 4 CPT FIETER MEMBRANE uccidere rayo ven app wea lesu ex ves pope tesa Er ed wet ales edu trae Deed eu pags Ra 5 COMPONENTS OF THE CONCENTRATING 6 3 1 DESCRIPTION OF COMPONENTS crea ore eie noa EE aora baa onore eara raru EPERE Kekse ob Rav EeNEYa PER Eo ERREUR KR 6 BASIC PROCEDURES 7 4 1 INSTALLING AND REMOVING THE PERMEATE LINE ccccssseccececeeseecececceeeceececeeaveceeceseueeeceeseseueeeecetauaneseseeea 7 4 2 INSTALLING AND REMOVING FLUID 7 4 3 INSTALLING AND REMOVING C
9. coming in contact with the instrument fluidics decreasing the need for between sample decontamination and eliminating cross sample contamination or carryover Extraction Port Front Filter Membrane Sample Port Permeate Port Back FIGURE 2 1 CONCENTRATING PIPETTE TIP CROSS SECTION Figure 2 1 shows a cross section view ot a disposable Concentrating Pipette lip CPT The filter membrane shown in green is hydrophilic a material that will allow air to flow through it only when the membrane is dry Once the filter has been wetted the surface tension of the liquid trapped in the pores of the membrane prevents air or any other gas from passing through the membrane Thus the CPTs cannot be re used since air trapped inside the housing cannot be pulled through the filter prior to processing a second sample The concentration process is performed in the following steps 1 The three way valve is configured such that the Extraction Port is turned off and the pump draws a vacuum on the Permeate Port The air inside the filter housing is drawn out and the liquid sample is pulled up through the Sample Port behind it A small air bubble trapped in the Extraction Port above the filter prevents the sample from coming in contact with the instrument s fluidics 2 As the sample is drawn through the filter particles larger than the chosen membrane pore size are captured on the front surface while liquids dissolved solids and particles
10. de NR NR Mineral spirits NR NR Copyright 2012 InnovaPrep www innovaprep com 13 R Recommended L Limited Exposure NR Not Recommended U Unknown 0 1 um Polyethersulfone Concentrating Pipette Tips 0 4 um Polycarbonate Track Etched PCTE Concentrating Pipette Tips 5 Nitric acid L L 25 Nitric acid NR NR Conc Nitric acid NR NR Perchloroethylene NR NR 0 5 Phenol NR NR 10 Phenol NR NR 0 1 N Sodium Hydroxide R R Conc Sodium Hydroxide NR NR Sodium hypochlorite 10 bleach solution L L 5 Sulfuric acid L L 25 Sulfuric acid NR NR Conc Sulfuric Acid NR NR Toluene NR NR Trichloroethane NR NR Trichloroethylene NR NR Triton surfactant solution R Tween surfactant solution R Water R Please contact InnovaPrep LLC for technical assistance and troubleshooting InnovaPrep LLC 132 E Main St Drexel MO 64742 Phone 1 816 619 3373 Fax 1 816 619 3377 info innovaprep com www innovaprep com Revision Date Author Rev 1 Dec 21 2012 A Packingham Rev 2 Jan 7 2013 D Alburty 3 Aug 15 2013 J Cawby Copyright 2012 InnovaPrep LLC www innovaprep com 14
11. e in some situations it may be more efficient to perform two 100 ms pulses rather than one 200 ms pulse while maintaining the same final elution volume Default 1 Max 256 Copyright 2012 InnovaPrep www innovaprep com 9 Flow buffer controls the amount of time the internal flow sensor must see zero flow before ending the run Setting it too low will end slower sample runs prematurely and setting it too high will cause the unit to continue to run long after all the sample has been processed The default value is 3 0 seconds do not change this unless slow sample runs are ending prematurely Min 0 2 Max 20 0 Reset all defaults resets all settings on the unit to the factory defaults including the decon procedure reminder 6 INSTALLING THE INSTRUMENT This section will take you through setting up the instrument in the laboratory Plug the included power supply into the 24VDC port on the side of the unit then plug the power supply into an available power outlet It is not necessary to select the correct voltage the sensor accepts line voltage of 100 to 240 VAC 50 60 Hz single phase The connection is self regulating Install the permeate line and run the tube into a drain or a waste container of appropriate size for sample volumes you plan to process It is recommended that you perform a decontamination cycle of the system before processing any samples See the Decon Cycle Section 7 1 1 for instructions Install an elution flu
12. e Adv options menu The following three menu items are more specific sub menu categories The second menu item is Procedures this sub menu contains important operational procedures for running the Concentrating Pipette 5 2 1 5 2 2 5 2 3 PROCEDURES The procedures menu contains three different procedures for Concentrating Pipette operation Extract Ext fluid prime and Decon cycle Extract performs a wet foam extraction according to the current instrument settings This can be used at any time to test the elution settings or perform additional extractions of a CPT Ext fluid prime is for priming the elution fluid lines when a new can is inserted This ensures that there is no air in the fluid path This option should also be used prior to processing the first sample of the day Since the extraction fluid contained in the unit s fluidics is not pressurized the CO will come out of solution and the fluid may not foam during the extraction cycle Decon cycle performs a routine decontamination of the entire fluidics system of the unit followed by a rinse The details of this procedure are described in Section 7 1 SYSTEM OPTIONS The System Options menu contains options for the three status LED s in the head as well as the beeper volume and the interval between decon reminders Decon reminder changes the number of days between automatic reminders to perform a decon procedure on the unit Default 14 days Beep volume changes the volume of
13. ed it is advisable to perform an Ext fluid prime before use to make sure fresh fluid is in the lines e Ensure the permeate fitting is fully seated in its quick connect port on the side of the instrument otherwise the fluid will not flow through the instrument Theinstrument can perform concentration cycles and extractions with the fluidics head in any position e Never run alcohol through the instrument as it may damage internal components e Use the knob on the left side of the arm to adjust how tightly the head is held in place Use the up and down V arrows to highlight options the screen and press enter lt to select To adjust settings press enter and use the up and down keys to adjust the setting to the value desired Then press the enter key again At the bottom of each menu tree the option Return is displayed selecting this option will return you to the previous menu Arun can be paused at any time by pressing enter You will be able to restart the run as long as no air is allowed to enter the tip Selecting End Run on the pause menu will allow you perform an extraction This function allows you to use one CPT to process a sample that is contained in more than one original container e final volume of your sample is controlled by the extraction fluid valve open time Generic small medium and large extraction fluid volume values are found in the options menu however under Adv Options you ca
14. en install the Rinse Fluid can Press Start When the 1 30 minute rinse cycle is complete the instrument will advance to the Procedures menu Remove and discard the Rinse Can and replace it with an Elution Fluid can After you perform an Ext fluid prime the instrument is ready to use Copyright 2012 InnovaPrep LLC www innovaprep com 10 7 2 SETTING UP THE EXTRACTION The Extraction is the most important part of the concentration process This will determine the volume of your concentrated sample and must also be setup properly to achieve the maximum possible efficiency Before performing a concentration run with a set of samples it is important to perform test extractions to ensure that the selected settings will produce the desired concentrate volume It is advisable to perform at least one test extraction at the beginning of the day after replacing an elution fluid can or after you change any of the extraction settings Test extractions should always be done with a CPT installed however performing an extraction with a new CPT will wet the filter surface and prevent it from being used for a concentration run for this reason it is advisable to keep a used CPT handy to perform test extractions without wasting new 5 The preset elution volumes of Small Med and Large are not given precise values because each instrument is slightly different These values correlate to roughly 320 uL 380 uL and 430 uL final volume The final volume can
15. iciently concentrates particles of interest from large liquid sample volumes Liters into liquid volumes as small as 200 microliters smaller volumes are attainable in some applications The Concentrating Pipette system uses a novel elution method to recover particles that have been captured onto a disposable porous membrane filter with very high recovery efficiencies FIGURE 1 1 INNOVAPREP CONCENTRATING PIPETTE 1 2 APPLICATIONS The patented InnovaPrep concentration technology has application anywhere that enrichment of low concentrations of particles is needed The primary area of application is for preparing and concentrating particles of biological origin including pathogens in liquid samples for subsequent analysis by microbiological methods or with microbiological detection devices 2 CONCENTRATING PIPETTE METHOD OF OPERATION The InnovaPrep concentration process uses dead end filtration to capture particles onto the surface of a porous membrane filter A novel Wet Foam Elution process is then employed to wash the particles off of the membrane surface into very small liquid volumes 2 1 CONCENTRATION PROCESS The concentration process used in the Concentrating Pipette is similar in many ways to the process used in other InnovaPrep concentrators however the method of drawing the sample into the disposable filter tip and eluting back Copyright 2012 InnovaPrep www innovaprep com 3 out the same end prevents the sample from
16. id can and perform an extraction fluid prime according to Section 4 2 The instrument is now ready to use 7 PROCEDURES This section will take you through the procedures required to operate the instrument including how to perform a concentration cycle 7 1 THE DECONTAMINATION CYCLE Biological contaminants can be damaging to the instrument if they are allowed to produce particles large enough to clog the fluid paths in the system The decontamination procedure is used to periodically ensure that no unwanted biological contaminants are contained in the fluid paths of the instrument To perform a decontamination cycle you will need a Maintenance kit P N HC08004 the Decon Tip Permeate Line Decon Tip Lubricant and a Waste Container Please note that a yellowing interior of the Decon Tip over time is normal With a gloved finger rub a very thin layer of lubricant on both ports of the Decon Tip Install the Decon Tip into the instrument using the same procedure described in Section 4 3 for installing a CPT Install the Decon Fluid can from the Maintenance Kit Place the end of the permeate line into an appropriate waste container Go to Main menu Options Procedures and select Decon Cycle Press Start When the ten minute decon cycle is complete the instrument will automatically begin Flushing When the flushing cycle is complete the instrument will beep and advance to the Rinse screen Remove and discard the Decon Fluid can th
17. is held in place 4 BASIC PROCEDURES This chapter will go over some of the basic procedures used later in the manual 4 1 INSTALLING AND REMOVING THE PERMEATE LINE Included with the instrument is a section of clear PVC tubing with a right angle quick connect fitting this is the permeate line To install the permeate line first make sure that the metal button on the Permeate Port is locked down on the instrument Then insert the quick connect fitting into the Permeate Port When the fitting is fully seated the metal button will pop up thus locking it in place To remove the permeate line simply press the metal button on the Permeate Port and it will be released 4 2 INSTALLING AND REMOVING FLUID CANS The port should be wiped with alcohol before use To install a fluid can into the instrument first hold the can with the valve pointing down Then place the can valve into the Can Interface Port on the right side of the instrument Now press the can down and hook the edge under the Can Latch as shown in Figure 3 1 Protect the Can Interface Port from contaminants by covering when not in use to or by keeping a can in place For priming the system after installing a new elution fluid can hold a waste container under the extraction fluid port under the fluidics head Go to Main menu Options Procedures and select Ext fluid prime Fluid will spray out of the extraction fluid port under the fluidics head This will purge the lines of air
18. luid passes from the high pressure environment on one side of the orifice to the low pressure environment on the other side the dissolved CO expands comes out of the solution to form micro bubbles These micro bubbles increase the volume of the fluid sevenfold or more An additional benefit of Wet Foam Elution is the buffer exchange In many situations the starting sample matrix is not the most desirable for the chosen analysis method Wet Foam Elution allows the user to select the fluid that the particles will be suspended in after concentration which maximizes the chances of detection 2 4 CPT FILTER MEMBRANE Selecting the proper filter media for a specific sample matrix is likely the most complex portion of the test setup The latest filter selection matrix is available on the InnovaPrep website Copyright 2012 InnovaPrep www innovaprep com 5 3 COMPONENTS OF THE CONCENTRATING PIPETTE The following section describes the components of the InnovaPrep Concentrating Pipette Fluidics Head Tip Interface Ports Single use Concentrating Pipette Tip Arm Tension Knob Can Latch a V A Fluid Can Power Switch USB Port Can Interface Port 24VDC Jack Permeate Port Sample Platform Control Panel FIGURE 3 1 CONCENTRATING PIPETTE COMPONENTS 3 1 DESCRIPTION OF COMPONENTS Description of components listed in Figure 3 1 clockwise from top e Fluidics Head This component contains the
19. n precisely control the number of milliseconds the valve is open as well as the number of pulses the valve performs The longer the open time and the more pulses performed the larger the extraction volume This function allows you to set up a calibration curve and set the instrument to produce a final volume close to the desired volume When adjusting the extraction volume be sure to perform a test extraction with a CPT installed otherwise you will not account for the volume of elution fluid that remains in the CPT at the end of an extraction cycle e Be sure that the tip is firmly clicked into position it will snap into the detent indicating that it is fully seated e Once a becomes wet it can no longer be used for testing Keep a used CPT handy to test extraction volume settings without wetting a new CPT e You can perform a test extraction by selecting Extract in the Procedures menu e f you run out of elution fluid during an extraction remove the concentrating tip replace the elution can perform an Ext fluid prime re insert the concentrating tip perform another extract e Please keep custom foam packaging insert for use in the event a return shipment is necessary 9 CHEMICAL COMPATIBILITY GUIDE When performing concentration cycles it is important to know the sample matrix This chart details how compatible the Concentrating Pipette fluidics and the membrane material in the CPT are with certain chemicals R R ded
20. o the final sample volume Copyright 2012 InnovaPrep LLC www innovaprep com 4 e Increased Viscosity Liquid has a tendency toward channeling when flowing across a surface that is there is an area of high flow in the center of the fluid path while the portion of flow in contact with the filter surface is much slower The higher viscosity of foam prevents channeling and creates a more uniform flow across the filter surface e Bubble Dynamics The micro bubbles in the foam behave as deformable solids As they travel across the surface of the filter they move as a ridged body with a narrow lubricating layer effectively squeegeeing the particles off of the surface e Exfoliating Action As the micro bubbles in the foam impact against each other and burst the turbulence and energy produced helps to lift particles that are adhering to the membrane 2 3 FOAM GENERATION The Wet Foam Elution process requires very specific high quality foam in order to be effective The elution fluid is composed of water a low concentration surfactant usually less than 0 196 and a pH buffer This solution is held at high pressure with carbon dioxide gas a significant amount of CO also dissolves into the fluid The fluid is plumbed to the extraction valve directly after the valve is a pressure orifice then the port leading to the filter During the extraction process the extraction valve opens forcing the elution fluid through the pressure orifice As the f
21. smaller than the chosen pore size pass through the filter and into the permeate 3 Once the sample container is empty air is drawn up behind the liquid and the membrane locks up leaving only the target particles on the front surface of the membrane and ending the run 4 When the user performs an extraction InnovaPrep s patented Wet Foam Elution process is utilized The Permeate Port valve closes and the extraction valve opens allowing the foam to enter the Extraction port The foam travels tangentially across the surface of the membrane as it washes the particles from the surface The concentrated sample is then pushed out of the sample port into a cuvette or other container where the foam quickly breaks back down into a small liquid volume 2 2 WET FOAM ELUTION The Wet Foam Elution process is similar to how liquid would be used to tangentially rinse particles off of a membrane filter however it is much more efficient than liquid rinsing for the following reasons e Volume Expansion When rinsing a filter with liquid most of the liquid volume is used to fill the dead space inside the filter housing only a small portion of the fluid is actually in contact with the filter surface This can be minimized to an extent by reducing the cross sectional area of the fluid path across the filter but a large portion of the liquid is still underutilized Foam however is 80 9096 gas which fills the empty space without contributing t
22. ss up on the head The thumbs up technique eliminates the need to hold the Fluidics Head with your free hand while you pull down on the CPT 5 NAVIGATING THE MENU SYSTEM This section will guide you through the instrument s LCD menu system navigation through the menu system is done with the three buttons Up Down and Enter 5 1 THE MAIN MENU When the instrument is first turned on a welcome screen will be displayed as soon as the unit boots up about 5 seconds Press enter to continue to the main menu screen The main menu consists of two selections Start Run and Options and a display of the length of the previous run If the instrument has just been turned on the length of the previous run will not be displayed since the instrument does not retain the value during a power down Selecting Start Run will begin the concentration process The screen will display a clock showing the elapsed run time Pressing enter will pause the run you will be given the option to continue the run or end the run Copyright 2012 InnovaPrep LLC www innovaprep com 8 Selecting Options will navigate to the main options menu which is separated into several sub menus 5 2 THE OPTIONS MENU The Options menu consists of four selections The first is Ext size which controls the final sample volume The four settings are small medium med large and custom cust The custom setting is shown only when the elution settings have been changed in th
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