1902 application guide
Contents
1. gt a F i 4 1 ei 4 a 4 t sd le s 8 ae 41 at a 2 ya a a os Sw 9 _ 4 44 3 al lt gt oe 4 5 ry F 4 lt e J a mal J Gel a ie J 9 4 6 ag Ss ve 7 A 4 or 3 oS x _ gt gt ed gt 1 931 4 4 Ni gt 2 Lat 2 1 F sank 5 re vf J iy gt edi 2 Paes es mI 4 ines ae HAY Sects e j 5 8 1 3 ys oe ee eels vd By 6 a Lee 22. 1902 application guide A guide to setting up CED 1902 amplifiers 03 9 2012 Cambridge Electronic Design Ltd Notes _ _ The 1902 amplifier is a versatile unit suitable for a wide vange of research applications that take recordings from surface or needle electrodes The 1902 Is a single channel modular unit that can be combined into stacks to give multiple amplifier channels All settings for the 1902 are controlled directly from within Spike2 and Signal or from a standalone amplifier control program if neither of the above are being used This guide is intended to help you setup a 1902 for recording Requirements CED 1902 amplifier Optional Spike2 or Signal for Windows Power1401 or Micro1401 laboratory interface Electrode adaptor box USB to serial adaptor The CED 1902 is sold as a research instrument and not as a medical device within the meaning of the EC Medical a eee Device Directive HeVICe VIVECUIVe CED 03 09 12 Contents What type of 1902 do need 1902 options by application e How many channels Connecting to the Isolated electrodes input CED1902 11 Electrode adaptor boxes CED1902 10 Active Head Stage e Wiring your own Isolated electrode input connection Transducer and Trigger inputs Software Installation Spike2 and Signal Signal conditioner support e Check for available COM ports Hardware configuration 1902 amplifier setup
3. 03 09 12 IF you know the COM port you want to use select it here and click Next IF you are not yet sure which port you want to use you can check for available COM ports as described below or select COM1 at this stage and then simply change the 1402 COM port in the GD mer section in Spike2 or Signal later on Check for available COM ports i veice Waneyer All available COM ports ave listed in the File Action View Help Windows Device Manager The quickest way t eee WARES A MAGIA qui 0 4 DAVID TOSH 1401 interlace open the Device Manager on any version of Potteries 0 i Computer Windows Is to use the Disk drives amp Display adapters keyboard shortcut to open the Run command ee oe 2 ILEATAATAPI enter devmgmt msc IN the dialog and Click OK I Imaging devices Keyboards 4 Mice and other pointing devices Be Monitors You can also open the Run command from the a 7 Ports COM amp LPT LPS Prolific US8 be Serial Comm Port COML Start menu in Windows XP In Windows 7 you A Processors amp Sound video and game comtrollers can access the Device Manager from the fia System devices Serial Bus comtrollers Control Panel in the Start menu or open the Run command from the Accessories folder in the Start menu In th
4. Control of the 1902 0 5 Spike2 The 1902 control panel is accessed from the Conditioner button in the GUID gict0g Doubte click on a channel in the QM sab of the Spike2 sampling configuration to open the Channel parameters IF a 1902 is connected to the 1401 port that ts selected in the Channel parameters dialog the Conditioner button will be enabled Click on this to open the 1902 controls During sampling you can access the 1902 control panel from the BARR menu 70 0 Signal The control panel for the 1902 Is available from the Port setup tab of the sampling configuration in Signal If you select an ADC port in the list and there is a corvesponding 1902 attached the CED 1902 button is enabled Click on this to open the 1902 controls During sampling you can access the 1902 control panel from the Sample menu option option IF Spike2 or Signal fail to detect the 1902 check that the COM port settings in the software match the COM port that the 1902 is connected to You set the COM port to use from the Edit Preferences menu Conditioner tab To check which COM port the 1902 is connected to see Check for available COM ports above 10 CED 03 09 12 The 1902 control panel To the right of the main control panel is an oscilloscope window which shows incoming data for the currently selected 1902 The control panel can also be accessed during sampling by selecting Signal conditioner from the Sampl
5. modular unit but they can be combined into stacks to give multiple channels or supplied as dual or quad channel variants with optional rack mounting Multiple stacks dual or quad channel units can then be daisy chained together to give the number of channels required Connecting to the Isolated electrode input When recording from electrodes attached to a subject the electrodes wust be connected to the isolated electrode input port of the 1902 There ave a number of connection options available depending on the number of channels and the electrode types being used Electrode adaptor boxes These are available in 2 4 8 or 16 channel variants with bipolar or common reference options and provide direct connection to the Electrode input sockets of multiple 1902 channels from one compact device The electrode connectors on the adaptor boxes accept 1 5mm touch proof electrodes Adaptors to connect 2mm electrodes can also be supplied if necessary CED 03 09 12 Active head stage The Active Head Stage also provides direct connection to the 1902 electrode input sockets and is available in a 2 or 4 channel version for use with concentric ov wire needle electrodes It provides differential amplification with switchable gains of x1 3 and x10 Wiring your own Isolated electrode input connection IF you are not using one of the CED1902 11 electrode adaptor boxes or the Active Head Stage you can wire your own connectors using t
6. 02 mk 4 detected and ready for use on eral port 5 channel 0 Serial number 2639 CPLD rey 3 monitor version 2 6 Communications test started Communications test stopped 0 errors in 69 passes 1902 calibration sequence started 1902 calibration sequence finished For Help press Fl Passes 69 Errors 0 Select the COM port and corresponding 1902 Channel from the drop down lists in the main toolbar of Tryt 902 A message at the bottom of the toolbar gives information about the detected 1902 If you are using multiple 1902 s you can check each channel for communication using the drop down list Once communication with the 1902 has been established select Set port and channels from the File menu in Try1 902 This allows you to set global settings for COM port and the first and last 1902 channels for use by the CtIL902 application 16 CED 03 09 12 By default the Try 1902 application will try and communicate with channel O as the first available channel on the 1902 If the first channel on your 1902 is not O you need to set the First 1902 channel to use option to match your first 1902 channel number Tryt 702 will then search up to the channel number specified in the Last field IF you are using multiple 1902 units where the channel numbers have a gap for example dual and quad channel units numbered 2 3 and 8 11 respectively you should set the Last field to match the highest channel number you want to
7. Control of the 1902 The 1902 control panel e Setting Gain and Offset e Filters e Triggering the Input clamp Standalone control of 1902 Troubleshooting CED 03 09 12 Page 5 4 LO 1 1 13 1 16 17 17 What type of 1902 do need 1902 options by application The following table shows the 1902 options that would typically be required for a range of vesearch applications This table is intended for use as a guide only IF you have other requirements or wish to check the suitability of the 1902 for your specific application you should contact CED directly A 7 tion Non lsolate d solated Standard gain Optional gain Optional Input Optional KLOO KLOOO clamp ECG tront K100 000 K1 000 000 end Transducer non invasive Transducer invasive MEP vecovaing x f f f x IMs x f f 9 4 gt lt 5 XS xX 6 XK KAS 2 x x potentials Including Force acceleration temperature strain and displacement transducers Standard gain is x1 x100 000 Iincludes Blood pressure or other invasive transducers that ave connected to a subject Input clamp is required if recording fast responses or from close to the site of stimulation ECG front end with 5 lead switching under software control The isolated input port of the 1902 will give the best results for most recordings due to its superior gain and low noise CED 03 09 12 How many channels Each 1902 is a single channel
8. e WMenu 1902 controls 0 005 Volt ort Inpu EEG uncamped Single ended ain i000 Offset Normal diff om 1000 Volt gt Inverted diff Trigger 2 1 Falling edge trigger Grounded EEG Resetcalibration Reload i902 sm Filters High pass Low pass Zeceswowh gt Cutoff 100 Hz Cutoff 500 Hz AC couple 50 Hz Notch EMis processing None 0 005 Volt 1 9242 006 Volt The oscilloscope window in the control panel is only shown if a 1401 is connected to the PC and switched on The Port list shows the current 1902 channel and the Input drop down list sets the input mode for the selected 1902 With an input clamp the list will display EEG unclamped and will also contain a list of times in milliseconds These specify the length of time to apply the input clamp in response to a pulse input on the Trigger 2 port of the 1902 If the clamp option is not fitted you should set the input to use Isolated EEG for recording from electrodes The EEG unclamped clamp duration settings and Isolated EEG options all use the isolated stage of the amplifier and ave the only inputs suitable for recording responses from surface electrodes 11 CED 03 09 12 Setting the Gain and 0 The Gain and Offset controls scale the incoming data The Reset calibration button can be used to automatically adjust the scale and offset val
9. e list of hardwave devices should be an entry for Ports COM amp LPT Click on this to show the available ports You can edit the COM number assigned to a port for most devices by double clicking on the entry Click on the Port Settings tab and then the Advanced button You can set the COM port number from a drop down list Once the 1902 conditoner support option has been selected you can continue with the installation There ave a number of other options some specific to Spike2 or Signal that you can select as required CED 03 09 12 Hardware configuration 6 aN 7 The diagram below shows the control line from the computer as either a direct serial port connection OR via a USB to serial converter This connection is made to the back of the 1902 amplifier unit or to the bottom wmost 1902 in a stack of multiple amplifiers For recording from surface electrodes on humans the isolated Electrode input of the 1902 wust be used Output of the amplified filtered signal is then produced at the Amp Out BNC connector Serial cable Opbonal USB S eral qonwerter 1902 rear 1902 front pare PSU input Electrode Transducer Amp out 1 connection to 1401 or other A D device Electrodes Optional adapter box CED 03 09 12 1902 amplifier setu With mains power off connect the power cable and included RS232
10. ger for 1902 input clamp Trigger 2 input Trigger pulse to Magstim triqgerea AFTER the 1902 input clamp Clamp duration Figure A Raw signal from electrode artefact 4 ee B Amplifier output Unclamaped Amplifier saturation causes first response to be missed aa 2 aati Fiqur Clamped output from 1402 Ay Deel etic Stimulus artefacts can often saturate the input of an amplifier causing a period of lost recovding while the amplifier input returns to a normal level This can cause problems if the response is very fast or is being recorded from close to the site of stimulation The diagram above shows a fast MEP response from the electrode Fig A that is missed due to the amplifier saturation caused by the stimulus artefact Fig B 14 CED 03 09 12 The CED 1902 amplitier can have a_stimulus clamp option titted that suppresses the artefact by clamping the amplifier input to ground during stimulation This prevents saturation of the amplifier input allowing fast responses to be vecorded Fig C The input clamp should be triggered marginally before the stimulator to ensure that the clamp circuit fully engaged before the stimulus is applied as in the above diagram Depending on the stimulator specifications one way to achieve this is to use a single pulse output of 0 5 to tms d
11. he supplied DIN plug included with the 1902 The Isolated electrode input is a G pin DIN socket which should be wired according to the pin descriptions in the 1902_ user manual 1902 can supply an excitation voltage suitable for invasive transducers and other devices that require connection between a human subject and the Isolated electrode input Contact CED for further details Transducer and Trigger inputs Wiring your own connection for the Non isolated transducer input The non isolated transducer input uses an 8 pin DIN socket and supplies excitation voltages suitable for driving non invasive transducers The wiring information can be found in the 1902 user manual Wiring your own connection for the Trigger inputs The Trigger inputs use a 4 pin mini DIN socket The wiring information can be found in the 1902 user manual Subjects must NOT be connected to the transducer input or Trigger ports CED 03 09 12 Software installation gt Spike2 and Signal Install the software from the supplied CD After setting the installation directory your name and organisation and selecting Compact Custom or Typical setup the following page will appear Signal conditioner support CED Installation Signal for Windows version 5 06 Setup Type 7 P Select the setup type that best suits your needs This option ts used to install software ft an 6 You may now choose what type of signal conditio
12. l you need to multiply this value by the full scale input 5 or LOV and enter it in the field in the Port setup Parameters dialog in Signal Once the correct units have been set you can then adjust the gain settings in the 1902 control panel to setup recording Any changes made to the gain settings from this point will automatically update the y axis display in the data file 12 CED 03 09 12 Filters The Filters section of the control panel is used to apply filtering to the incoming data High and Low pass filters You can specify the type of filter to use and the frequency cut off values from the drop down menus for both high and low pass filters You can also type your own values into the Cutoff fields if the value you want to apply is not displayed in the drop down list Notch filter The notch filter is set to remove a single frequency and is usually set to the local mains power supply SO or GOHz depending on location The Notch filter is only provided for diagnostic purposes and should not be used during experiments AC couple This option blocks the DC component of a waveform and can be thought of as a high pass filter with a cutoff of O LGHz This can be useful if your feature of interest is small compared to the large DC level Unlike the High and Low pass filter settings which are applied at the amplifier output the AC couple ts applied at the input 13 CED 03 09 12 Triggering the input clamp Trig
13. ner support to install Pl that the Power 401 with ADC shoice is only useful if support for a signal conditioner along required for sampling using a Power 4 1 without ADC gain with Spike2 or Signal Select CED Nore CED 1902 signal conditoner d kk Axon Instruments Cyberdmp signal conditioner an C IC Powel 401 with ADC gain option Digitimer DS60 signal conditioner Information on setting up a 1902 amplifier for recording can be found in the 1902 amoplifier setup section Install Sie lt Back Next gt Cancel CED Installation Signal for Windows version 5 06 The CED 1902 amplifier is COM port selection Selec the sr re to use controlled via a COM port The software uses a serial line to control the signal conditioners 4 2 5 Many deskto Pp P C us wil Select the COM port to use of leave unchanged if this ts already set or you do not know which port you will be using 00M 1 have at least one COM port 12 coms cOM13 available usually COM1 For coms cons laptops and PCs without an 6 16 ee available COM port a USB to serial 19 adaptor can be used instead This should be plugged in to an available ase USB port 6 6 continuing CED
14. serial cable to the rear of the 1902 Connect the power cable to a power outlet and the RS232 cable to an available COM port on the host PC or to the USB serial adaptor that you connected to a USB port on the host computer during software installation Switch on the mains outlet to power up the 1902 Once the green Power LED is lit the 1902 Is ready for use IF you are using Spike2 ov Signal and the 1902 conditioner support option was selected during the installation complete control over the 1902 amplifier settings is provided via a control panel which can be used to adjust amplifier gain and offset modify filter settings and enable triggering and clamp duration if the 1902 has been fitted with the input clamp option If powered up and connected to the PC the 1902 will be detected when Spike and Signal ave first run By default the control software expects each 1902 channel to be connected to the corresponding ADC port number on the 1401 interface using BNC cables which you should connect now This example uses a single 1902 channel 0 which is connected to ADC O Don t want to use the ADC port on the 1401 that matches the 1902 channel ae number by default For instance you may already be using ADC Inputs O 2 but want to connect a 1902 which is set to channel O to ADC Input 3 To do this you need to change the 1902 channel number to match the ADC Input using the rotary switch on the rear panel of each 1902 channel CED 03 09 12
15. ues in the sampling configuration so that the incoming data is displayed in units of Volts millivolts or microvolts depending on the gain you have selected in the 1902 control panel Whenever the gain or offset is changed in the control panel Spike2 and Signal will adjust the gain and offset settings in the sampling configuration to keep the y axis display showing the same units in the data file but for this to update automatically you first need to set how the incoming data should be scaled into the y axis units Using the Reset calibration option IF you have set the units to Volts millivolts or microvolts in the sampling configuration you can use the Reset calibration button Adjust the gain and offset controls so that the largest waveform feature you want to vecord fits in the oscilloscope display window without clipping and then click the Reset Calibration button During recording any changes to the gain will be automatically applied to the y axis display in the data file Want to use different y axis units IF you want to use different units of measurement you first need to set these in the sampling configuration For example you may be recording from a force transducer that has an output of 0 1525 Newtons per mV and want to apply this scale to the data file In this case you need to express the transducer output in terms of Units per Volt 152 5 which can then be set in the QED cic in cnc tf you are using Signa
16. uration and route this to both the trigger input of the stimulator and the Trigger 2 input using a T piece The same pulse can then trigger the 1902 clamp with the rising edge of the pulse and the stimulator with the falling edge ensuring the necessary delay Alternatively you can set up a second pulse on another digital output to trigger the 1902 clamp circuit separately from the stimulator When using the input clamp the active head stage should not be used You should also turn off the AC couple option and set the 1902 high pass filter setting to None 15 CED 03 09 12 Standalone control with 1902 and 20 1 90 Don t have Spike2 or Signal In this case you can use the diagnostic program Try2902 and standalone amplifier control program CtIL902 supplied on the installation CD shipped with the 1902 These applications provide the necessary setup and control applications for standalone 1902 use and are installed in a folder called CED 1902 Support available from the Windows Start menu Open the 1902 program to set the required COM port and communications for the 1902 To check which COM port the 14902 is connected to see Check for available COM ports above a Try1902 Version L28 19 January 2012 File Edit Wew Help Com port Corr 1902 Channel 0 Calibrate amplifiers Interactive mode Vary parameters Lonmmunications test Trigger test EEPROM test 19
17. use Click OK to confirm these settings and then exit the 21902 program before opening 0011902 from the CED 1902 Support folder This opens a control panel similar to that supplied in Spike2 and Signal but without the oscilloscope display of incoming data See The 1902 control panel section for details on how to set inputs gain and filter settings Troubleshooting A diagnostic program 1902 Is included on the 1902 installation CD and can be used to provide details of any problems Double check that the 1902 Is connected to the PC via a COM port or USB serial interface and open the 1902 program which can be found in the 1902 folder and accessed from the Start menu The main toolbar displays the current communication status of any detected 1902 s and also contains drop down lists for COM ports and 1902 channels from which you can select a 1902 channel to check If a 1902 channel has been detected there are also options to perform Communications Trigger and EEPROM tests and to Calibrate Theve is also an Interactive mode with which individual software commands can be sent to the 1902 for diagnostic purposes If you are still having problems establishing communications with the 1902 you should contact CED directly for help either by phone or email at hardhelp ced co uk 17 CED 03 09 12 wu we he S ae hex 7 4 r 3
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