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USER MANUAL - Loligo Systems

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1. The software will save all input data and calculated values to an Excel data file Page 3 of 19 3 2 LoliTrack software for Windows The following steps will explain how to install LoliTrack on your computer 1 Insert the CD labelled LoliTrack and wait until you see Screen 1 If you do NOT see the screen browse the CD and double click on the icon labelled LT install exe Screen 1 WinZip Self Extractor LT install exe LA Unzipping Loli Track by Loligo Systems Un zipping LWRTNBFIFO msi 2 Click Next to start installation of LoliTrack Screen 2 wi LoliTrack Welcome to the LoliTrack installer Itis strongly recommended that you exit all programs before running this Installer Applications that run in the background such as virus scanning Z utilities might cause the installer to take longer than average to complete Page 4 of 19 3 Select destination directory for LoliTrack and LabView drivers and then press Next Screen 3 W LoliTrack Destination Directory Select the primary installation directory All software wall be installed in the following location s To install software into a different locationis click the Browse button and select another directory Directory for LoliT rack C Program Files Loli Track Directory for National Instruments products C Program Files National Instruments rr 4 If you accept the License Agreement please select I accept the License
2. Agreement s and then press Next Screen 4 LY LoliTrack License Agreement ou must accept the licenze displayed below to proceed INSTALLATION NOTICE THIS 1S A CONTRACT BEFORE YOU DOWNLOAD THE SOFTWARE AND OR COMPLETE THE INSTALLATION PROCESS CAREFULLY READ THIS AGREEMENT BY DOWNLOADING THE SOFTWARE AND OR CLICKING THE APPLICABLE BUTTON TO COMPLETE THE INSTALLATION PROCESS YOU CONSENT TO THE TERMS OF THIS AGREEMENT AND YOU AGREE TO BE BOUND BY THIS AGREEMENT IF YOU DO NOT WISH TO BECOME A PARTY TO THIS AGREEMENT AND BE BOUND BY ALL OF ITS TERMS AND CONDITIONS CLICK THE APPROPRIATE BUTTON TO CANCEL THE INSTALLATION PROCESS DO NOT INSTALL OR USE THE SOFTWARE AND RETURN THE SOFTWARE WITHIN THIRTY 30 DAYS OF RECEIPT OF THE SOFTWARE INCLUDING ALL ACCOMPANYING WRITTEN MATERIALS ALONG WITH THEIR CONTAINERS TO THE PLACE YOU OBTAINED THEM ALL RETURNS SHALL BE SUBJECT TO NI S THEN CURRENT RETURN POLICY Definitions As usedin this Agreement the following terms have the following meanings y accept the License Agreement sl A do not accept the License Agreement s 5 Click Next Page 5 of 19 On Screen 5 the status of the installation is shown The installation may take a while Screen 5 W LoliTrack Overall Progress Currently installing Loli Track Part 1 of 1 it Back i Screen 6 Ll LoliTrack Installation Complete The installer has finished updating your system Some of the
3. Select Install driver 21 Choose language and then click Next 22 When Screen 15 appears click Next Screen 15 IDS uEye Welcome to the InstallShield Wizard for uEye Please read the file Whats ew tet before working with Eye You can choose to read the file at the end of the Installation process Also visit Http wii uepre com for the latest drivers Page 11 of 19 23 When Screen 16 appears select Complete and then click Next Screen 16 IDS uEye Setup Type Select the setup type that best suits pour needs Click the type of setup you prefer Se os Eciton 2 USB and Gigabit Ethernet Installation of all components 3 USB 4 Custom 24 Choose a destination and then click Next 25 Select a program folder and then click Next 26 Click Install to start the installation 27 Select the additional features you want then click Next Screen 17 Additional features Select or clear additional features Install a desktop shortcut to WEye Demo and to yEye Camera Manager Install a quick launch shortcut to uEye Demo and uEve Camera Manager Read the latest information about this driver release Whata ew tet 28 Select Click Finish to end the installation of the uEye software package 29 Remove the CD labelled LoliTrack from your CD drive 30 Restart the Computer You have now finished the installation of all the software Page 12 of 19 3 3 uEye USB camera OPTIONAL 1 C
4. installed products require activation Activate these products nowy 6 Click Next to end installation of LoliTrack and activate the product This activation requires an internet connection Page 6 of 19 7 Choose automatic activation and click Next to activate the software Screen 7 Ml Activation Wizard Welcome to the National Instruments Activation Wizard Complete the Activation Wizard to activate your product Activation is the process of acquiring and applying an activation code 2 Automatically activate through a secure Internet connection 5 Use a Web browser email client telephone or fax machine to acquire an activation code O Apply a 20 character activation code previously acquired For this computer Understanding the National Instruments Software License Agreement How can I move an activated product to a different com uber 8 Enter the serial number found on the LabView Vison runtime license card Then click on Next Screen 8 NI Activation Wizard Activating LabVIEW 8 2 Development System Enter the serial number supplied with your product Serial Number The serial number can be found on your product s Certificate of Ownership or packaging Page 7 of 19 9 Fill out the fields with your information when done click Next Screen 9 NI Activation Wizard Activating LabVIEW 8 2 Development System The Following information is required to activate your product First Marn
5. File gt Exit Page 15 of 19 4 3 Details 4 3 1 General Image Here the video images are shown When maximizing LoliTrack on your PC monitor the image will scale accordingly Move your cursor over the image and zoom unzoom by using the scroll button on your mouse Binary image Here the binary image is shown according to the threshold values When maximizing LoliTrack on your PC monitor the image will scale accordingly Move your cursor over the image and zoom unzoom by using the scroll button on your mouse Current When clicking on image objects the colour reading in the position of the mouse cursor is shown Save button Clicking on this button will save the current pixel colour and use this to threshold video images Saved Shows the saved colour value Hysteresis This field is used to allow a range of pixel colours in order to account for multi coloured objects and or changes in light conditions during the experiment Example The Loligo Systems logo has a blue colour with a value of 16860 Separating this value into RGB will result in red 0 blue 220 and green 65 In the binary image to the right all pixels with this exact value will be shown as red pixels on a black background If the Hysteresis value is then set to 10 using the slide bar all pixels with a colour between red 0 10 blue 210 230 and green 55 75 will appear as red pixels Zones Enable to view any zones of interest defined by the user Trace sec Set
6. USER MANUAL LoliTrack 2 0 1 List of parts o LoliTrack installation CD o USB hardkey dongle WIBU o LabView Vision runtime license o UuEye camera VE10250 OPTIONAL o User manual Page 1 of 19 2 Contents 1 USOS ooo iaa idas 1 Za COMES A iia 2 IMStalla tion proceda reacia ra 3 dal GONG Ml PA Un hase cele T N E meena nesta E TE 3 3 2 LoliTrack software for WINdOWS emma 4 3 3 uEye USB camera OPTIONAL aia 13 4 USING EOI TS A AS AA AAA a 14 4 1 Sta UD lito dolo dias 14 4 2 QUICK Starlsi ir A ci 15 4 3 Det aaa 16 4 3 1 General A o O OO 16 4 3 2 RIE MEN Ureno e a O a A ON a Ta 17 4 3 3 SCUINGS Mentor aa 18 4 3 4 Cal Ciel surta tao tad 19 4 3 5 HED Mentir A TE AA E EEA 19 Page 2 of 19 3 Installation procedure 3 1 General LoliTrack is a piece of PC software for video based tracking of an object LoliTrack require Windows XP or Vista running on a PC with an Intel Pentium IV processor of 1 GHz and 1 GB RAM or better We recommend monitors with a minimum resolution of 1024x768 pixels LoliTrack requires a mouse with a scroll button One free USB ports is required on your PC for connecting the hardkey dongle The LoliTrack software finds the position of an object e g experimental animal on the principle of contrast between the object and its surroundings by frame by frame analysis The software calculates Velocity Acceleration Distance moved Active time Zones defined by user O O O O O
7. e Last Mame Organization Ei Jessen Loligo Systems ApS Register my product to Facilitate technical support and ensure I receive important updates From National Instruments Country Denmark Address i Agro Business Park Address cont NES sales City State Postal Code te Sd E Email Phone Fax ej loligosystems corn 45859992546 45859992599 National Instruments Product Activation Privacy Statement 10 If activation was successful Screen 10 appears Click on Finish to end the activation Screen 10 NI Activation Wizard Activating LabVIEW 6 2 Development System Product Successfully Activated The Following product has been successfully activated You may need to restart the associated product in order to use any new Functionality LabYIEW 8 2 Full Development System with MathScript IF ou experience any problems or have any questions or feedback regarding product activation visit the Following Web site ni compactivate I want to register my NI hardware Page 8 of 19 11 Now install drivers for the hardkey dongle When Screen 11 appears click Next Screen 11 2 WIBU KEY Setup This program will install WIBU RE Y Software on your qa Welcome to the WIBL REY Software Setup program computer Itis strongly recommended that you exit all Windows programs and the control panel before running this Setup program Click sCancele to quit Setup and then clase any program
8. iles after the setup process Bact Next gt Cancel 18 Select unpack and remove and then click Next Screen 13 dl uEye InstallShield Wizard Remove Installation Files Do you want to remove the Files used to perform the installation f Unpack the files used to perform the installation to a temporary location and automatically remove these files after the setup is completed Select this option if you are not planning to run the setup again later f Unpack the files used to perform the installation to the location specified below and dont remove these files after the setup is completed Select this option if you are planning to run the setup again at a later time IF the specified folder location does nat exist twill be created automatically save files in folder To continue click Next lt Back Cancel Page 10 of 19 19 Wait for the files to be unpacked The setup screen will appear when done Screen 14 9 uEye SDK 3 30 Webstart Welcome to the installation program For the 105 vEyve camera Family Please check first whether the hardware of your camera is compatible with this driver version Wihakshew txt Show File with the latest information regarding this driver release Check hardware Check the compatibility of your camera hardware with this driver version oCheckUSBbus Show all USB controllers Install driver Start the setup program For the driver installation noise g 20
9. of 19 4 2 Quick start Open either an existing video file avi from File gt Open AVI file or start analyzing live video images from an USB camera Camera gt Open camera Place your mouse cursor on the object that should be tracked in the left side screen Then click the Save button to store the colour of the particular object In the right screen you will now see pixels of that colour only and marked with red The Hysteresis bar below can be used to choose a range of colours to account for changes in lighting and reflections x position pixels Acceleration pix s s 1 Current x y position pixels Acceleration pix s s 80 335 1 am on 1 341 365 0 9 Velocity pix s Active time s E ih Velocity pix s Active time s h J3 1 A lo 26 3 s Zone A EE 0 5 10 15 20 25 Tracking the blue object Tracking the green object Now click Calibration gt Pixel and use the mouse to draw a line along an axis of a known distance in the image Then enter this distance and unit of measure The pixel to meter ratio is now calculated Click OK when done Now LoliTrack will find the position of the animal and calculate its velocity the distance moved etc To start writing experimental data to a file click File gt Save data file Choose a destination for the data file A raw and a calculated data file will now be written until the user stops saving data or exits the program To exit LoliTrack click
10. onnect the uEye USB camera to the PC After a few seconds the software installation will start automatically 2 Click on Install the software automatically Recommended and then on Next 3 Click on continue anyways 4 Click on Finish 5 Open the uEye camera manager by clicking Start gt All Programs gt IDS gt uEye Screen 18 Avail Type Cam ID Dew Ib Model OI 154x M 40027085319 Control center _ Expert mode Create support File ETH network service Additional Functions Automatic ETH configuration Status of device Mr 1 The camera is configured correctly and can be opened 6 If installation was successful the uEye camera is now shown here 7 Mount the lens on the uEye camera 8 The uEye camera is now ready for use with LoliTrack Page 13 of 19 4 Using LoliTrack 4 1 Start up Start LoliTrack by clicking Start gt All Programs gt LoliTrack gt LoliTrack If the USB hardkey dongle is not connected to the PC an error dialog box pops up see Screen 19 Screen 19 Lolilrack exe Start Error ES Copy protection error Mo license Found 2699 140000 caca Connect the USB hardkey dongle to your PC and wait for the device to be recognized then click on Retry Then Screen 21 will appear Screen 20 LoliTrack 2 0 File Settings Camera Help x y position pixels Acceleration pix s s Zones 0 0 0 Trace sec Velocity pix s 0 Distance moved pix 46339 5 Page 14
11. rs are prompt to enter the time and date for the first frame In this way the software will keep track of the real time instead of using the pc clock when time stamping X and Y coordinates Exit On exit the software will close the camera input and save all settings and then close LoliTrack Page 17 of 19 4 3 3 Settings Menu Mask Opens a dialog box where users may create a mask to avoid analysis in certain areas Use either one of the upper buttons to draw a closed figure in the image Then decide if you want to include or exclude the enclosed area by pressing either one of the two upper buttons in the lower group of buttons Below there is a button to undo the last step and one to clear the entire mask and start over Also it is possible to invert the drawn mask Created masks can be saved or loaded as bitmap files When maximizing the dialog box the image will scale When moving the mouse over the camera it is possible to zoom unzoom the image via the scroll button on the mouse Zones Opens a dialog box where users may define zones of interest in which the presence of the tracked object will be scored automatically by the software Start by drawing a closed figure Then dedicate the closed figure as a zone by clicking on one of the numerated buttons When finished drawing zones LoliTrack will ask if user wants to save the zones A dialog will open and choose current folder Zone 1 will be saved as Zone1 bmp etc When maximizing
12. s you have running Click Nexte to continue with the Setup program Version 5 0b Cancel 12 Select supported language and click Next 13 Click Next without changing any options 14 Click Next again after you have accepted the summary of the installation 15 When installation is done click Next again 16 Click Finish to end installation of the WiBu drivers Page 9 of 19 17 Now drivers for the optional uEye USB camera will be installed This installation is required even if you want to use another kind of camera When Screen 12 appears click Next Screen 12 ll uEye InstallShield Wizard Welcome to the self extracting uEye installation WEye 33000 exe is a self extracting archive The files will be saved in the folder you are asked to enter The default folder will be lt TEMP JEYE_ 33000 lt TEMP ia temporary directory selected by the system The following directories wil be created in the specified folder CheckUSa This directory contain a program that show s all USB controllers of your computer system Hardware Check This directory contains a program that checks if your hardware is compatible with this driver Feadite This directory contains text files with the latest information regarding the installation and the uEye driver Seige This directory contains the installation program for the uE ye driver setup exe Please note SOU can choose not to delete the Installation f
13. the USB cameras Video settings Opens a dialog where user can set the video settings for the camera Image settings Opens a dialog where user can set the image settings for the camera 4 3 5 Help Menu About This menu will open a dialog box showing the version number of the LoliTrack software and contact information for Loligo Systems Page 19 of 19
14. the dialog box the image will scale When moving the mouse over the camera it is possible to zoom unzoom the image via the scroll button on the mouse Filter Opens a dialog box where user may set several filter options to improve image analysis The pixel noise can be used to avoid scoring activity or movement due to image noise Example If pixel noise is set to 2x2 the x y position of the object must change with more than 2 pixels in each direction before LoliTrack counts the change in position as a real movement of the object Object Opens a dialog box where users may set object tracking settings Also the time for calculated data can be set here Calibration Opens a dialog box where users may convert image pixels into mm cm or metres Change the length and or position of the green line to match any known distance in the image e g a ruler The length of the green line will show Then choose the desired unit of measure and enter the known distance A pixel ratio will now be calculated and used for all calculations When maximizing the dialog box the image will scale When moving the cursor over the image it is possible to zoom unzoom the image via the scroll button on the mouse Page 18 of 19 4 3 4 Camera Open camera Opens the uEye camera connected to the PC If no uEye camera is found or the camera is already in use a dialog will open and show all available USB cameras connected to the PC The user can then choose on of
15. trace length in seconds FPS Shows current frame rate in frames per second Frame rate depends on camera settings the PC and filter methods chosen X y position pixels Shows current X and Y coordinates object The upper left corner is defined as 0 0 Page 16 of 19 Velocity Shows calculated velocity Velocity will be calculated in either pixels s m s cm s or mm s depending on the unit of measure chosen in Calibration Distance moved Shows the distance moved by the object from the tracking was started Distance moved will be calculated in either pixels m cm or mm depending on the unit of measure chosen in Calibration Acceleration Shows the calculated acceleration Acceleration will be calculated in either pixels s s m s s cm s s or mm s s depending on the unit of measure chosen in Calibration Active time Shows the active time of the object from the tracking was started Zone Shows the current zone of the object When the object is not in a defined zone a zero will be shown Angle Shows the calculated angle or direction of movement of the object 4 3 2 File Menu Load AVI file Use this menu option to open an AVI file for analysis Save data file Stop data file This will open a dialog where the user can enter name and destination of data files Two files will be written a calculated data file and a raw data file On stop both files will be closed and can be opened in e g Excel When analyzing AVI files use

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