Mixer M-925
Contents
1. 12 40 V DC from the pump requirement Power 1W consumption Mixer M 925 Instructions 56 8101 00 Edition AE Inlet and outlet UNF 10 32 thread profile tubing e g Fingertights for capillary tubing 1 16 outer diameter Dimensions Hx Wx D 152x 77x 111 mm Weight 1 2 kg 6 3 Accessories and spare parts Item Quantity Code no per pack Mixer M 925 including 1 18 1118 89 one UniNet cable Cable UniNet 0 7 m 1 18 1109 74 Mixing chamber must be ordered separately 90 ul 1 18 1147 24 0 2 ml 1 18 1147 21 0 6 ml 1 18 1118 90 2 ml 1 18 1118 91 5 ml 1 18 1118 92 12 ml 1 18 1118 93 Stop plug 1 16 5 18 1112 52 Fingertight connector 10 18 1112 55 PEEK for i d 1 16 tubing Sleeve for 18 1147 10 10 18 1156 63 PEEK o d 1 16 i d 305 pm Sleeve for 18 1147 10 10 18 1147 11 for capillary with i d 0 10 mm Sleeve for 18 1147 10 10 18 1147 12 for capillary with i d 0 15 mm Union 2 18 1120 92 1 16 male 1 16 male i d 0 25 mm Union 2 18 1120 90 1 16 male 1 16 male i d 0 13 mm Mixer M 925 Instructions 56 8101 00 Edition AE Mixer M 925 Instructions 56 8101 00 Edition AE Important user information All users must read this entire manual to fully understand the safe use of Mixer M 925 WARNING The WARNING sign highlights instructions A that must be followed to avoid personal injury It is important not to proceed until all stated conditions are met and clearly understood Caution The2. Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product Declaration of conformity Safety standards This product meets the requirements of the Low Voltage Directive 73 23 EEC through the harmonized standard EN 61 010 1 1993 A2 1995 EMC standards This product meets the requirements of the EMC Directive 89 336 EEC through the harmonized standard EN 61 326 1 1997 A1 1998 The CE symbol and corresponding declaration of conformity is valid for the instrument when it is connected to other CE marked Amersham Biosciences instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from Amersham Biosciences except for alterations described in this manual WARNING This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures Terms and Conditions of Sale All goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Biosciences group which supplies them A copy of these terms and conditions is available on request Should you have any com
3. 1 Introduction Mixer M 925 is a dynamic single chamber mixer with interchangeable mixer volumes The mixer is used in stand alone applications with AKTA design Pump P 900 series in AKTAdesign chromatography systems and in Ettan liquid chromatography systems Features e 2 step mixing for optimum results e Flow rates up to 100 ml min s e Interchangeable mixing chambers with volumes of 90 pl 0 2 0 6 2 5 or 12 ml 1 1 Safety WARNING When using hazardous chemicals suitable protective measures such as protective glasses must be taken WARNING Mixer M 925 with 12 ml chamber must not be used at pressures above 10 MPa 100 bar 1450 psi Mixer M 925 with 90 ul or 0 2 ml mixer chamber must not be used at pressures above 35 MPa 350 bar 5075 psi 2 Installation 2 1 Unpacking CAUTION Before connecting the Mixer M 925 ensure the power is switched OFF at the system pump or the complete system 56 8101 00 Edition AE Mixer M 925 Instructions Mixer housing Chamber lock Unpack the mixer and check the items against the packing list Inspect the items for obvious damage which may have occurred during transportation 2 2 Installing the mixer CAUTION Do not install the mixer closer than 0 5 m from any computer display The rotating stirrer may disturb the display screen The mixer and the mixer chamber s are delivered in separate pa
4. ckages and must be assembled before use 1 Connect the mixer with two UniNet cables or a termination plug UniNet 2 as a part of the chain UniNet 2 chain l we M a Le lt 1 Amersham 2 Mount the mixer in an AKTAdesign or ak an Ettan liquid chromatography system Mixing Recommended flow rates or together with an AKTAdesign P 900 chamber ml min series system pump in stand alone volume and Two pump Switch valve applications See the user documentation M2X pressure gradients gradients for further information 90 1 35 MPa 0 0025 2 0 J hamb pose fans 0 2 ml 35 MPa 0 0025 5 0 3 a E eA Mes OLING 0 1 5 2 m1 25 MPa 0 5 100 1 10 5 ml 25 MPa 5 100 5 30 2 3 Fitting mixing chamber 12 ml 10 MPa 15 100 WARNING When using hazardous Note For BufferPrep select flow rates as for chemicals ensure that the mixer chamber switch valve gradients has been flushed through with distilled water before removing an used chamber A mixer chamber must be fitted to the mixer Ce housing prior to use To fit a mixer chamber 1 Open the plastic lock covering the mixer chamber compartment A spring is securing the mixer chamber in position when the lock is opened 2 Insert a mixer chamber and close the Mixer chambers of 90 ul 0 2 0 6 2 5 and lock 12 ml are available 3 Fit the inlet and outlet Recommended flow rates for each mixing tubing Use Fingertight sis chamber are specified in th
5. cription A mixer motor inside the housing spins a magnet at 600 rpm that causes the stirrer in the mixing chamber to rotate The eluents are mixed in two steps 1 Premixing in a static mixer with a small volume 22 ul 2 Dynamic mixing chamber with rotating stirrer The mixer housing and the mixer chamber contains no user replaceable items Tt Mixer M 925 Instructions 56 8101 00 Edition AE 6 2 Technical specifications Operating data Max flow rate 0 001 100 ml min Max pressure 12 ml chamber 10 MPa at 0 1 100 ml min 100 bar 1450 psi 0 6 2 and 5 ml 25 MPa at 0 001 100 ml min chambers 250 bar 3625 psi 90 ul and 0 2 ml 35 MPa at 0 001 5 ml min chambers 350 bar 5075 psi pH range 1 13 1 14 lt 1 day exposure Viscosity Max 5 cP Environment 4 to 40 C 20 95 relative humidity 84 106 kPa 840 1060 mbar atmospheric pressure Physical data Internal volume 6 interchangeable mixing chambers 90 ul 0 2 0 6 2 5 and 12 ml Mixing principle 1 static chamber and 1 dynamic chamber Degree of IP 43 protection Wetted materials Stirrer PTFE polytetrafluorethylene Mixing PEEK polyetheretherketone chambers Chemical The wetted parts are resistant resistance to organic solvents and salt buffers commonly used in chromatography of biomolecules except 100 ethyl acetate 100 hexane and 100 tetrahydrofuran THF DH stability range 1 13 1 14 lt 1 day exposure Power
6. e table below connectors or a Union male male according to When using eluents that are more difficult to the recommendations in mix such as isopropanol and water a large the system mixer volume can be used to get optimum documentation mixing Mixer M 925 2 Instructions 56 8101 00 Edition AE 3 Operation The mixer is controlled from a system pump in the AKTAdesign P 900 series or from P 905 in an Ettan liquid chromatography system and is by default operating whenever the pump is running See the User Manual for the pump The mixer can be run without liquid but it should be avoided due to mechanical wear 3 1 Storage Overnight The mixer can be left filled with a buffer Overnight and long term storage Flush the mixer with water and then fill with 20 ethanol 4 Maintenance CAUTION Only spare parts approved or supplied by Amersham Biosciences may be used for maintaining and servicing Mixer M 925 4 1 Periodic maintenance Period Action Every 6 months General inspection Every 2nd year Replace the mixer chamber 4 2 Cleaning in place Pump a cleaning or sanitizing agent through the mixer The standard recommendation is to pump 1 M NaOH 1 ml min for 30 minutes and then wash out with buffer WARNING NaOH is injurious to health Avoid spillage Mixer M 925 Instructions 56 8101 00 Edition AE 4 3 General inspection 1 Check that the mixer chamber is clean and without dama
7. ge Check the tubing connectors Replace if required 4 4 Replacing mixer chamber WARNING When using hazardous chemicals ensure that the mixer chamber has been flushed through with distilled water before removing an used chamber 1 Make sure the pump is stopped 2 Place the buffer bottles lower than the mixer to prevent draining and then remove the inlet and outlet tubing 3 Open the plastic lock holding the mixer chamber A spring is securing the mixer chamber in position when the lock is opened 4 Pull out the mixer chamber gently 5 Insert the new mixer chamber and close the lock 6 Replace the inlet and outlet tubing 5 Trouble shooting If the suggested actions do not correct the fault call Amersham Biosciences Fault Action Leakage 1 Check the tubing and connectors If leakage is located to the mixer chamber replace the complete chamber Waves on the gradient 1 Check that the pump is operating and is programmed correctly 2 Clean the mixing chamber to make sure it is free of dirt or particles 3 Change to a larger mixing chamber volume if necessary Unlinear gradients or slow response to B changes 1 Check that the tubing has been washed properly and that the pump is operating 2 Change to a smaller mixer volume Not running 1 Check cables 2 Check Setup Mixer menu on the pump Other faults Contact Amersham Biosciences 6 Reference information 6 1 Des
8. ments on this product we will be pleased to receive them at Amersham Biosciences AB SE 751 84 Uppsala Sweden Trademarks Ettan UNICORN and AKTA are trademarks of Amersham Biosciences Limited Amersham and Amersham Biosciences are trademarks of Amersham ple Office Addresses Amersham Biosciences AB SE 751 84 Uppsala Sweden Amersham Biosciences Amersham Place Little Chalfont Buckinghamshire England HP7 9NA Amersham Biosciences Corp 800 Centennial Avenue P O Box 1327 Piscataway N J 08855 USA Amersham Biosciences Europe GmbH Munzinger Strasse 9 D 79111 Freiburg Germany Amersham Biosciences Sanken Building 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Copyright Amersham Biosciences AB 2001 All rights reserved lt 4 Amersham TC information ab Uppsala Printed by TK Tryck AB Uppsala November 2001
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