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1. 7 Fro tine Quanti 1000 015 8 The Frontline Quanti Dispenser 9 SYLINGE AAA R Qa we ce a 10 Syringe Pump Dispenser ae 10 CHAPTER TWO DISPENSER INSTALLATION U u u u u u u u 11 Background s EE LLL eee 11 Key a 11 Tools Equipment 12 ise ui 7 12 BioJet Quanti 3000 12 B gforzro eT EL LL 12 Installation ERE 13 AirJet Quanti 3000 2300 and 2400 Installation u XR Sa Dua ak a D D GR 15 BioDot Dispenser Operating Manual Version 1 5 Table of Contents ii Bree f 2 ua
2. 68 INDEX d P 69 BioDot Dispensers B Operating Manual Version 1 5 Introduction How This Manual Is Organized Page 1 INTRODUCTION HOW THIS MANUAL IS ORGANIZED F This manual is both a tutorial and reference guide for the BioJet Quanti AirJet Quanti Frontline and Syringe Pump dispensers The information is applicable to all BioDot dispensers in any feasible application This manual is designed to supplement the operating manual for a BioDot system platform or BioDot in line production system that contains a dispensing module If you are not using your dispenser with a BioDot platform any necessary modifications will have been made at the factory and you should have received any necessary supplementary information If you find that the information here is insufficient or not applicable to your particular dispensing application please contact BioDot Support Services see Appendix D The organization style of this manual is similar to that of the BioDot system platform manuals The information here is organized into chapters the order of which corresponds to the steps in the basic sequence for the installation operation and maintenance of BioDot dispensers Each chapter contains a sub section that is specific for each type of dispenser so that the user may easily acces
3. 15 15 Frontline Dispenser InstallatiOn 5 o nnn aah neat ae neat ae Ae a Suo iv v futu is ee en ee eats 17 Unpacking UP RE 17 17 e 18 ERR 18 Installatl ERSTE DD 19 CHAPTER THREE DISPENSER OPERAT IQN L UU stsenssesneisnscesneisncensaunueasausancswauauucav enaA a enaa aa aada aaa aaa EaR 21 Background a E ai 21 Key 0 o RES 21 PYOCGGUICS u unata Qanpa Qasa 22 Dispense Cycle uU pei 22 Navigauno the Menus ur uu EL D ccc eta LLLI UM MM LM NS 22 T 23 EE 23 DISPOSE geo c ays 23 Programmis 23 Menu Hierarchies eee 23 Entering and SAVING 27 Creating Deleting Programs ebbe 27 PROGRAM and CONFIGURATION the Dispenser Programming 28 REPEATS and PRIME DISPENSER the Accessory Dispensing 29 Editing EXISTING Pr
4. CHANNEL 1 gt X OFF mm 9 00 VALUE Y OFF mm 0 00 VALUE TPU CHANNEL 0 VALUE l AIRJETQUANTI 1 gt CHANNEL INFO SYRINGE uL 250 VALUE FILL uL 250 VALUE TYPE XP3000 XL3002 XL3004 XL3005 XL3006 XL3008 XL3000 STD XL3000 HI MODE HIGH RES LOW RES FORCE HALF QUARTER FULL ADDRESS 1 VALUE COM BIOS COM 1 COM 2 SOLENOID E CHANNEL 1 gt X OFF mm 62 00 VALUE Y OFF mm 0 00 VALUE SOLENOID gt OUTPUT 0 VALUE BioDot XYZ S Series Menu Tree BIODOT Operating Manual Version 1 5 Page 27 Entering and Saving Data There are essentially three options when you are entering data you will CREATE a new program EDIT an existing program or DELETE an existing program The key to entering data is this you must immediately save any new program data or changes you make to existing programs by selecting SAVE DATA under the DATA STORAGE menu ALL data will remain in working A memory until it is formally saved in this manner Warning If the unit looses power any new programs will be lost if not saved Creating and Deleting Programs To create a new program you must first enter a name for the program After the name is saved into memory you will then enter the desired web handling parameters for that program under the EDIT sub menu 9 Note Creating the first program on
5. I uu u uu u u u 60 BioJet Quanti Line Dispensing Parameters 50 100 uL Syringe 0 2 to 5 0 uL cm Dispense 61 BioJet Quanti Line Dispensing Parameters 250 500 uL Syringe 0 375 to 10 0 uL cm Dispense 62 Quanti Line Dispensing Parameters 1 0 ml Syringe 1 0 to 25 0 uL cm Dispense 63 APPENDIX C DISPENSER OPTIONAL COMPONENTS I 64 Line Presence Inspection Syste 65 us nem 65 m a m m 65 Basic Installation 8 67 Contact 68
6. X START mm This field allows you to program the X axis starting point relative to the coordinate origin home in millimeters Y START mm This field allows you to program the Y axis starting point relative to the coordinate origin home in millimeters Z UP mm for 3050 amp 3200 only Displayed on units with a motorized Z axis only This field allows you to program the start and end position in the Z axis relative to the coordinate origin home in millimeters Zero represents the upper most position Z DOWN mm for 3050 amp 3200 only Displayed on units with a motorized Z axis only This field allows you to program the dispense position in the Z axis relative to the coordinate origin home in millimeters Zero represents the upper most position DROP nl This field allows you to program the volume of each drop Please note that when the operator enters a volume the software will round the value down based on the RATE DROP PITCH and ON TIME A variety of volumes BioDot Dispensers B Operating Manual Version 1 5 Page 36 are achievable and the BioJet Dispenser Rate Tables indicate this information DISPLAYED FOR DOT SHAPE ONLY TOTAL nl This field is displayed for the DOT task only This field is not editable and displays the total volume delivered for each dispense The total volume is the product of the drop volume see above and the total
7. 27 Editing Programs 30 Entering Saving Data 27 2 Z and Z Down 40 BioDot Dispensers Operating Manual Version 1 5 BIODOT
8. Dispensers BioJet Quanti 3000 AirJet Quanti 3000 2400 2300 Frontline Quanti 1000 Syringe Pump Operating Manual Revision 1 5 BIODOT 17781 Sky Park Circle Irvine California 92614 TEL 949 440 3685 FAX 949 440 3694 http www biodot com E mail support biodot com Table of Contents Table of Contents INTRODUCTION HOW THIS MANUAL IS ORGANIZED u u u u u u u u 1 CHAPTER ONE DISPENSER 5 uvae i uda cuve Ce esae ra gere 3 is ieeiuo 3 Key WV OIG ies M 3 BioJet 0 5 3 3000 REN 4 The BioJet Quanti Dispenser n 4 Pn Weir Im uu uuu W m aqu 6 Mei Eee LIU LI DEDE 6 AirJet Quanti 2300 L U anette a eee eee a r aaa 6 AirJet Quanti 2400 nnn Un eee 7 The AirJet Quanti Dispenser
9. NO LENGTH mm 300 0 VALUE POLORIZATION NO YES SPEED mm s 50 0 VALUE ACC mm s2 1000 VALUE X START mm 0 0 VALUE Y START mm 19 0 VALUE Z UP mm 0 0 VALUE Z DOWN mm 55 0 VALUE REPEAT 1 0 VALUE AIRJETQUANTI 1 BIOJETQUANTI 1 e BIOJETQUANTI 2 c BIOJETQUANTI 2 gt ACTIVE YES NO RATE uL cm 1 008 VALUE DROP nl 31 3 VALUE PITCH mm 0 31 VALUE ON TIME ms 0 25 VALUE r BIOJETQUANTI 1 gt ACTIVE YES NO RATE uL cm 1 008 VALUE DROP nl 31 2 VALUE PITCH mm 0 31 VALUE ON TIME ms 0 25 VALUE AIRJETQUANTI 1 gt ACTIVE YES NO RATE uL cm 10 00 VALUE PATTERN NAME DATA STORAGE CHECK MEMORY SYSTEM INFO e WATCH INPUTS INPUTS 1 ACTIVE 012345678 000000000 HIT ESC TO EXIT F SYSTEM INFO gt SERIAL 0 VALUE BIOS V1 23 VALUE FIRMWARE V2 156 VALUE BDLIB V1 23 VALUE KERNEL V1 21 VALUE AXIS LIB V1 354 VALUE NOTE VALUES OF 65535 MEANS THERE IS NO VALUE CONFIGURATION tp CONFIGURATION EDIT DISPENSER e EDIT AXES TRGR BIAS ms 65 VALUE HOME mm s 40 0 VALUE JOG mm s 80 0 VALUE mm s2 1000 VALUE Z SPD mm s 200 0 VALUE Z ACC mm s2 2000 VALUE PRIME uL s 100 0 VALUE FILL uL s 10
10. LENGTH 200 or NAME MYPROGRAM A new value is entered into an edit field by selecting the field with the Up T or Down J arrow keys and then typing the new value using the keypad Toggle Field A Toggle Field is similar to an edit field except that the associated data are selected from a fixed group of choices A new choice is selected by using the Up Down arrow keys to move the menu selector to the field to be changed Activated This term describes a toggle field which is set to YES or ON Pitch The drop pitch describes the distance in millimeters between drop centers Rate The dispense rate is the volume of reagent dispensed per centimeter in a dispensing program expressed in ul cm Drop Volume The drop volume is the volume of each drop dispensed expressed in nl On Time The on time is the length of time in milliseconds that a dispenser valve stays open during each unit of a dispensing sequence Procedures Running a Dispense Cycle The dispense cycle begins and ends with the hand held terminal Data are entered via the keypad the menu structure stored data and unit status are displayed on the LCD Please refer to your user manual for a full description of the keypad Navigating the Menus Four keys are used to move through the menu hierarchy The UP T and DOWN Jarrow keys move the menu selector up or down one line The entire menu will scroll upward or downward if the selector is already at its top or bottom position and
11. gt EDIT TANDEM BJQ gt EDIT TANDEM CAVRO gt EDIT CAVRO gt EDIT AIRJET gt AXIS 2 gt NAME Y PC 104 BUS CPU ADDRESS 768 VALUE UNITS mm MOTOR STEPS 200 VALUE uSTEPS 64 VALUE TRAVEL 17 731232 VALUE TOP SPEED 181 VALUE NEG LIM 0 00 VALUE POS LIM 78 00 VALUE ENCODER NONE REF ONLY EM AXIS 1 gt NAME X CPU BOARD PC 104 BUS UNITS mm MOTOR STEPS 200 VALUE uSTEPS 64 VALUE TRAVEL 17 731232 VALUE TOP SPEED 181 VALUE NEG LIM 0 00 VALUE POS LIM 495 00 VALUE ENCODER NONE REF ONLY 1 EDIT BIOJETQUANTI gt BIOJETQUANTI 1 BIOJETQUANTI 2 BIOJETQUANTI 2 gt CHANNEL INFO SYRINGE uL 250 VALUE FILL uL 250 VALUE TYPE XP3000 XL3002 XL3004 XL3005 XL3006 XL3008 XL3000 STD XL3000 HI MODE HIGH RES LOW RES FORCE HALF QUARTER FULL ADDRESS 2 VALUE COM BIOS COM 1 COM 2 L CHANNEL 1 gt X OFF mm 0 00 VALUE Y OFF mm 0 00 VALUE TPU CHANNEL 1 VALUE E BIOJETQUANTI 1 gt CHANNEL INFO SYRINGE uL 250 VALUE FILL uL 250 VALUE TYPE XP3000 XL3002 XL3004 1 3005 XL3006 XL3008 XL3000 STD XL3000 MODE HIGH RES LOW RES FORCE HALF QUARTER FULL ADDRESS 1 VALUE COM BIOS COM 1 COM 2
12. repeat COLUMNS A field used to define the number of columns to be used for the pattern program repeat X mm A field used to define the repeat offset for the X axis in millimeters Y mm A field used to define the repeat offset for the Y axis in millimeters PRIME DISPENSER The PRIME DISPENSER menu is available in all platforms and is used to prime any of the available dispensers A dispenser should be primed in preparation for dispensing or cleaning When the operator selects PRIME they will first be prompted to select a dispenser type and then the device number from left to right where there are more than one of that type of dispenser Finally the operator will enter the number of prime cycles for the dispenser selected The operator then selects and activates the ACTIVATE field to start the prime cycle at the prime speed set under the configuration menu see PROGRAM amp CONFIGURATION the Dispenser Programming Menus For BioJet dispensers the solenoid valve will operate during the prime cycle at a factory set RATE and ON TIME that is appropriate for the PRIME cycle The dispenser will continue to prime until the operator presses ESC on the hand held terminal or the dispenser has completed the number of cycles Note For any dispenser requiring the syringe pump the first prime after the unit has been activated will not stop when the ESC key is pressed The first prime after activation is an initialization prime that tells the syr
13. 40 25 28 0 20 50 31 0 50 16 67 0 42 0 20 58 33 0 42 0 58 33 33 42 0 20 66 67 42 0 70 5 10 42 0 21 0 20 1 5 33 33 0 22 0 25 7 16 66 24 0 20 1 8 37 5 21 0 30 16 67 0 33 0 20 50 00 0 33 0 50 25 36 0 20 50 28 0 50 25 00 0 50 0 20 75 00 0 50 0 70 33 33 48 0 25 75 42 0 70 6 12 50 0 21 0 20 2 0 50 00 0 25 0 48 8 16 67 21 0 20 2 0 41 67 21 0 40 16 67 0 28 0 20 75 00 0 33 0 70 25 31 0 20 66 67 33 0 67 25 00 0 42 0 20 100 00 0 50 0 80 33 42 0 25 100 5 0 80 7 14 58 0 21 0 20 3 0 75 00 0 25 0 70 9 25 28 0 20 3 0 66 67 22 0 67 16 67 0 24 0 20 100 00 0 33 0 80 37 5 42 0 30 100 33 0 80 33 33 0 48 0 25 150 00 0 50 0 90 50 56 0 50 150 5 0 90 8 16 67 0 21 0 20 4 0 100 00 0 25 0 80 1 0 20 83 21 0 20 4 0 100 25 0 80 25 00 0 31 0 20 133 33 0 33 0 87 33 33 33 0 25 133 33 33 0 87 33 00 0 42 0 25 200 00 0 50 1 00 50 5 0 50 200 5 1 00 9 18 75 0 21 0 20 5 0 125 00 0 25 0 85 1 1 25 23 0 20 5 0 125 25 0 85 25 00 0 28 0 20 166 67 0 33 0 93 33 33 3 0 25 166 6 33 0 93 33 33 0 37 0 25 250 00 0 50 1 10 50 45 0 50 250 5 1 10 BioDot Dispensers Operating Manual Version 1 5 BIODOT Page 62 BioJet Quanti Line Dispensing Parameters 250 500 uL Syringe 0 375 to 10 0 uL cm Dispense Rate 250 uL Syringe 500 uL Syringe RATE DROP DROP ON RATE DROP DROP ON RATE DROP DROP ON RATE DROP DROP ON VOLUME PITCH TIME VOLUME PITCH TIME VOLUME PITCH TIME
14. VOLUME PITCH TIME ul cm nl mm msec ul cm nl mm msec ul cm nl mm msec ul cm nl mm msec 375 10 42 28 0 20 2 75 83 33 33 0 75 375 20 83 56 0 20 2 75 83 33 30 0 76 20 83 56 0 20 114 58 42 0 80 41 67 1 11 0 40 125 00 45 0 85 31 25 83 0 25 125 00 50 0 85 62 50 1 67 0 67 250 00 91 1 11 50 10 42 21 0 20 3 00 93 75 31 0 78 50 20 83 42 0 20 3 00 125 00 42 0 83 20 83 42 0 20 125 00 42 0 83 41 67 83 0 40 166 67 56 0 94 31 25 63 0 25 166 67 45 0 94 62 50 1 12 0 65 250 00 83 1 10 75 20 83 28 0 20 3 25 125 00 38 0 85 75 20 83 28 0 20 3 50 125 00 36 0 86 31 25 42 0 25 135 42 42 0 88 41 67 56 0 40 166 67 48 0 95 41 67 56 0 40 250 00 77 1 11 62 50 83 0 65 250 00 71 1 10 1 00 20 83 21 0 20 3 50 125 00 36 0 86 1 00 20 83 21 0 20 4 00 166 67 42 0 96 31 25 31 0 25 166 67 48 0 95 41 67 42 0 40 250 00 63 1 10 41 67 42 0 40 250 00 71 1 11 62 50 63 0 65 333 33 83 1 18 1 25 31 25 28 0 25 3 75 156 25 42 0 92 1 25 41 67 33 0 40 5 00 208 33 42 1 00 41 67 37 0 40 187 50 50 0 98 62 50 50 0 65 250 00 50 1 10 62 50 56 0 66 250 00 67 1 11 83 30 67 0 75 500 00 1 00 1 28 1 50 41 67 28 0 40 4 00 166 67 42 0 96 1 50 62 50 42 0 57 6 00 250 00 42 1 08 62 50 42 0 65 250 00 63 1 10 83 33 56 0 76 333 33 56 1 17 83 33 56 0 76 333 33 83 1 18 125 00 83 0 85 500 00 83 1 28 1 75 41 67 24 0 40 5 00 208 33 42 1 00 1 75 62 50 36 0 65 8 00 333 33 42 1 17 62 50 36 0 65 250 00 50 1 10 83 33 48 0 75 50
15. a system platform where there are no programs in memory i e when you first receive from the factory a system platform that has no sample programs entered for you requires a different procedure than that for the creation of subsequent programs BioDot Dispensers To create the first program name on a system platform Select DATA STORAGE then PROGRAMS then CREATE all platforms Type in a program name using the terminal keypad and press the ENTER key when finished To create a new program name Select CREATE under the PROGRAM Reel to Reel platform or PATTERNS XYZ or ZX platform menu Type in a program name using the terminal keypad and press the ENTER key when finished Immediately save the program name using the ESC key return to the main menu and then select SAVE DATA under the DATA STORAGE menu To enter the program parameters for the new program Return to the PROGRAM or PATTERNS menu and select EDIT Review the EDIT sub menu in the previous menu trees Use the arrow keys to select menu options where you wish to enter data Enter new data in each menu option field Press the ENTER key after each completed entry SAVE the program Using the ESC key return to the main menu and then select SAVE DATA under the DATA STORAGE menu B D O Operating Manual Version 1 5 Page 28 Your new program is now saved in non volatile memory To delete a program e Select DELETE under the PROGRAM Reel to Reel platf
16. margin icon and highlighted text For example The goal of the background section is to provide you with a context for the information that follows BioDot Dispensers B O T Operating Manual Version 1 5 Introduction How This Manual Is Organized Page 2 Notes Notes are given throughout the manual For example Note Notes call your attention to vital information or key instrument features A Warning Warnings are in bold text and are presented in a block format where a potentially hazardous process is described For example A Warning Failure to read your manual will greatly impair your ability to operate and maintain your dispenser s Orienting Terminology Directional terminology is used throughout the manual to illustrate the relative positions of the system platform components We have defined this set of terminology below to ensure that these terms are clear to you when you encounter them in your reading 9 Note Except where indicated directions are given from the point of Looking Down o Unit view of a machine operator who is a facing the front of the machine and b running web material in the standard direction Back side from left to right Upstream Refers to elements on the left hand side or to the left Upstream lt Platform Unit Downstream relative to other elements Downstream Refers to elements on the right hand side or to the right relative to other elements F
17. number of drops delivered see below For example if you enter VOLUME 104 nL and DROPS 10 then this field will display 1040 nL DROPS This field is displayed for the DOT task only This is the total number of drops to be dispensed FREQ Hz This field is not displayed for the LINE task The frequency or number of droplets emitting from the BioJet Quanti can be selected up to 1000 Hz VOLUME ul This field allows you to program the volume per dot in ul and appears when the SHAPE menu has been toggled to the dot mode This field is available for Syringe pumps or AirJet dispensers only You should now be ready to operate your dispensers In the next section we discuss specifically the operation of the individual dispensers At the end of the chapter we have included three test programs for the BioJet dispenser The test programs serve a dual purpose they are diagnostic programs and will determine proper functionality of the BioJets and because it is advisable to perform these tests after BioJet installation they provide programming examples and practice to the new user BioJet Quanti 3000 Operation General Operation The BioJet Quanti dispenser is based on the combination of a solenoid valve to form drops and a syringe pump to meter reagent The BioJet Quanti takes advantage of the high resolution stepper motors that drive the syringe pump by synchronizing the stepper increments with the opening and closing of the soleno
18. or totally clogged Disconnect the dispenser from all electrical power before sonicating The BioJet should generally not be sonicated unless clogging occurs Back flushing Back flushing simply means to push fluid through the BioJet in the reverse direction from that which is normal The procedure is as follows 1 Remove the BioJet from its housing you can do this by removing the tip pulling back on the mechanical spring on the top of the housing and then sliding the BioJet out of the housing 2 Disconnect the tubing from the top of the BioJet but leave the electrical lead connected 3 Obtain a syringe and a blunt 21 gauge needle the 21 gauge needle fits very snugly inside the end of the BioJet If you do not have this you can use tubing that will fit over the syringe end and over the entire BioJet end You need to create as snug a connection as possible 4 Fill the syringe with water and carefully insert the needle into the end of the BioJet 5 Run several PRIME cycles and when the BioJet is open to allow fluid through i e when the syringe is dispensing slowly push the water from the syringe through the BioJet It may require some pressure at first to unstick the poppet Once the poppet is unstuck water should drip from the inlet nozzle of the BioJet 6 If water comes through the inlet nozzle then reconnect the tubing and tip to the BioJet as usual and run a prime to verify that fluid can prime through Note If something ha
19. s will have been modified to accommodate the system a housing for the reflectance sensor and fiber optic wires should be located on the right side of each BioJet dispenser 3 Adjust the height of each BioJet to its production height dispensing height determined by your specific process specifications Note system must be re calibrated whenever dispensing height is adjusted to account for changes in the reflectance sensor depth of field 4 Insert the fiber optic sensor on the right hand side of each BioJet 5 Ensure the observed dot of light is in sharp focus on the substrate web If the dot of light appears diffuse adjust the height of the sensor within the slide housing using the small screw on the rear of the dispenser to loosen and then re secure the sensor Figure 12 6 Orient the sensor so that the receive side solid black fiber optic wire is located downstream from the transmit side dotted fiber optic wire the receive side of the sensor should be located on the inspection sensor side of each dispenser 7 Calibrate the reflectance sensor As calibration requires the exposure of the sensor to the same membrane when wet and when dry membrane with a dispensed line vs membrane with no dispensed line this is most easily accomplished while the system is running e While dispenser is running a light can be observed on the front of the LPIS amplifier press the SET button on the LPIS ampl
20. the DATA STORAGE menu Program A program is a storage location in non volatile memory for a set of patterns that comprise a web handling process Pattern The word pattern signifies a programmed series of dispensing actions which is part of a dispensing program The menu title PATTERN found in XYZ ZX platform software is used interchangeably with the PROGRAM menu title found in Reel to Reel platform software in that they are both used in the creation or editing of programs Keypad The Keypad is the set of buttons on the hand held terminal Menu A Menu is a specific grouping of information and fields One menu at a time can be displayed Menus that are longer than 4 lines are partially displayed on the LCD The hidden part of the menu can be displayed by scrolling the text up or down with the arrow keys Menu Structure The menus are arranged in a hierarchical tree structure The highest level is called the main menu From this menu you can access only sub menus at different levels in the hierarchy the ESC key is used to return to the previous higher level menu Field Fields are the areas within each line of a menu where data can be entered BioDot Dispensers B Operating Manual Version 1 5 Page 22 Menu Selector The Menu Selector is the indicator arrow gt that points to the active menu element Edit Field An Edit Field is a field that contains an editable numeric or textual value after the field name i e
21. the regular weekly cleaning protocol in addition to the standard weekly procedure It is of particular value in helping to prevent cross contamination when dispensing multiple reagents from a single dispenser or to clean protein buildup from the lines and the valve Note You must validate the decontamination procedure to ensure its adequacy in your own application Purge supply lines of reagent Prime 3 cycles of deionized water with 0 05 BioTerge through the system Prime this solution out of the system Prime Jetwash into the system and allow the solution to sit in the BioJets for 30 minutes Prime this solution out of the system Clean and refill the supply reservoir with deionized water Prime dispenser for 5 10 syringe cycles Remove the glass syringe from the syringe pump Remove the plunger from the syringe and flush deionized water through the open syringe and wash the plunger in deionized water being careful not to damage the plunger seal 10 Flush deionized water through the ports in the 3 port valve The valve stem may be turned by hand to switch path positions SOOO c ON CD BioDot Dispensers B Operating Manual Version 1 5 Page 49 Unclogging Procedure If clogging of the BioJet should occur then the following procedures may be followed Sonication The entire dispenser housing when removed from the supporting arm may be sonicated in deionized water if the dispenser becomes partially
22. there are additional menu items above or below the currently selected one Note The RIGHT and LEFT lt arrow keys not used e The ENTER key will activate the currently selected menu item and either bring up a sub menu or toggle between fields in a toggle field e The ESC key displays the menu above the current one in the hierarchical structure Pressing ESC will ultimately bring the operator to the Main Menu BioDot Dispensers B I T Operating Manual Version 1 5 Page 23 Starting a Cycle The dispense cycle once programmed into working memory is executed by pressing the GO key on the hand held terminal The platform will sequentially scan each of the patterns in working memory in order and dispense each selected pattern If pattern repeat is in use see REPEAT in EDIT Sub Menu Options Defined later in this chapter the platform will move to each grid element and dispense all enabled tasks Aborting A Cycle A dispense cycle may be interrupted at any point by pressing the ABORT key on the hand held terminal Any dispensing activity will immediately cease and the slides will be returned to the park position Alternatively the operator may use the PAUSE MODE key which allows the operator to pause the platform either before or right after the dispense sequence The operator can then press GO to resume or ABORT to stop 9 Note Using the PAUSE key to pause the platform is useful in confirming dispenser positio
23. ul cm This field allows you to program the volume of liquid dispensed per unit length expressed in and applies when the SHAPE menu has been toggled to the LINE option This field is available for Syringe pumps or AirJet dispensers only REPEAT 1 REPEAT 1 is used along with the X and Y start positions versus dot pitch for the LINE task to repeat an individual pattern Several dots or lines can be drawn in a single pattern line allowing more flexibility in building complex dispense patterns The REPEAT 1 function is used along with REPEAT 2 to create two dimensional patterns a matrix or grid pattern For an example see the Dot Dispense Test under Test Programs for BioJet Dispensers at the end of this chapter REPEAT 2 REPEAT 2 is used along with REPEAT 1 This is a secondary level of individual pattern repeat that can be used to dispense a matrix of dots or lines For an example see the Dot Dispense Test under Test Programs for BioJet Dispensers at the end of this chapter REPEAT Z This is a toggle field YES NO used to activate the REPEAT Z function When activated the Z axis will raise to its Z UP position between each dispense within an executed program When off the Z axis will remain in the Z DOWN position between each dispense Activating this option is useful for example if the dispenser must be raised out of a microtitre well between each dispense DISPLAYED FOR BOTH LINE and DOT SHAPE
24. 0 VALUE TANDEM AJQ 1 CHANNEL INFO SYRINGE uL 250 VALUE FILL VOL uL 250 VALUE TYPE XP3000 XL3002 XL3004 XL3005 XL3006 XL3008 XL3000 STD 1 3000 HI MODE HIGH RES LOW RES FORCE HALF QUARTER FULL ADDRESS 4 VALUE COM BIOS COM 1 COM 2 SOLENOID e SOLENOID OUTPUT 1 VALUE CHANNEL 1 X OFF mm 0 00 VALUE Y OFF mm 0 00 VALUE EDIT PIN ARRAY Operating Manual Version 1 5 BioDot Dispensers BIODOT XYZ S SERIES V2 156 FACTORY MODE PRESS ENTER MAIN MENU PATTERNS REPEATS MM PRIME DISPENSERS PRIME DISPENSER BIOJETQUANTI DEVICE 1 VALUE ACTIVATE GROUP NO YES CYCLES 1 VALUE ACTION PRIME EMPTY AIRJETQUANTI ACTIVATE CYCLES 1 VALUE ACTION PRIME EMPTY REPEATS gt STATUS DISABLED ENABLED ROWS 1 VALUE COLS 1 VALUE X mm 0 0 VALUE Y mm 0 0 VALUE LLL PATTERNS gt e EDIT e SELECT DELETE 6 DELETE PATTERN gt TEST e DELETE PATTERN TEST PRESS TO CONFIRM OR ABORT TO CANCEL SELECT PATTERN gt TEST EDIT PATTERN gt TEST p PROGRAM 1 NAME TEST ACTIVE YES SHAPE LINE DOT Z ENABLED YES
25. 0 0 VALUE FILL SYRINGE NO YES INSP SENSORS Z UP ms 0 VALUE Z DOWN ms 0 VALUE Z CYLINDER e EDIT DISPENSER AIRJETS 0 VALUE CAVROS 0 VALUE AIRJETQUANTI S 1 VALUE BIOJETQUANTIS 2 VALUE TANDEM CAVROS 0 VALUE TANDEM BJQ S 0 VALUE TANDEM AJQ S 0 VALUE PIN ARRAYS 0 VALUE EDIT AIRJET EDIT CAVRO SIMULT DISP NO YES DISP LIB V1 42 VALUE r Z CYLINDER gt OUTPUT 65535 VALUE MEMORY AVAILABLE CURRENT 10412 Wer SENSORS gt SENSORS 0 VALUE DATA STORAGE gt PROGRAMS SAVE DATA EDIT AXES gt AXES 3 VALUE STORING DATA BONE AXIS 2 AXIS 3 PROGRAMS gt CREATE DELETE PC 104 BUS CPU ADDRESS 772 VALUE DELETE PROGRAM 1 UNITS mm PRESS GO TO CONFIRM MOTOR STEPS 200 VALUE OR ABORT TO CANCEL USTEPS 64 VALUE TRAVEL 17 731232 VALUE r SPEED 181 VALUE FILE NAME NEG LIM 0 00 VALUE POS LIM 60 00 VALUE ENCODER NONE REF ONLY EDIT AIRJETQUANTI Page 26 EDIT BIOJETQUANTI EDIT TANDEM CAVRO EDIT TANDEM BJQ EDIT TANDEM AJQ e EDIT PIN ARRAY e BIOJET DRIVERS 2 VALUE BJ PROFILE NPB STD EDIT PIN ARRAY gt EDIT TANDEM AJQ
26. 0 00 63 1 25 83 33 48 0 76 500 00 1 00 1 28 125 00 71 0 86 666 67 83 1 37 2 00 62 50 31 0 65 6 00 250 00 42 1 08 2 00 62 50 31 0 62 10 00 500 00 50 1 30 83 33 42 0 75 333 33 56 1 17 83 33 42 0 75 666 67 67 1 33 125 00 63 0 85 500 00 83 1 28 125 00 63 0 85 1000 00 1 00 3 00 2 25 62 50 28 0 70 8 00 333 33 42 1 17 2 25 83 33 37 0 74 15 00 625 00 42 1 33 83 33 37 0 74 500 00 63 1 25 125 00 56 0 86 1000 00 67 3 00 125 00 56 0 86 666 67 83 1 37 187 50 83 0 98 2 50 83 33 33 0 70 10 00 500 00 50 1 30 2 50 83 33 33 0 75 20 00 1000 00 50 3 00 104 17 42 0 80 666 67 67 1 33 125 00 50 0 85 125 00 50 0 85 1000 00 1 00 3 00 166 67 67 0 95 BioDot Dispensers Operating Manual Version 1 5 BIODOT Page 63 BioJet Quanti Line Dispensing Parameters 1 0 ml Syringe 1 0 to 25 0 uL cm Dispense Rate BioDot Dispensers 1 0 ml Syringe RATE DROP DROP ON RATE DROP DROP ON VOLUME PITCH TIME VOLUME PITCH TIME ul cm nl mm msec ul cm nl mm msec 1 00 41 67 0 42 0 40 4 00 166 67 0 42 0 96 83 33 0 83 0 75 250 00 0 63 1 10 125 00 1 25 0 85 333 33 0 83 1 18 1 25 41 67 0 33 0 40 4 50 187 50 0 42 1 00 83 33 0 50 0 75 250 00 0 56 1 10 125 00 1 00 0 86 375 00 0 83 1 18 1 50 83 33 0 56 0 76 5 00 208 33 0 42 1 00 125 00 0 83 0 85 250 00 0 50 1 10 166 67 1 11 0 96 500 00 1 00 1 22 1 75 83 33 0 48 0 75 6 00 250 00 0 42 1 08 125 00 0 71 0 86 333 33 0 56 1 17 250 00 1 43 1
27. 00000 VALUE TOP SPEED 260 VALUE NEG LIM 0 00 VALUE POS LIM 9999999 0 VALUE ENCODER REF ONLY NONE COUNTS 1 VALUE ACCURACY 0 VLAUE EDIT DISPENSER AIRJET 0 VALUE CAVROS 0 VALUE AIRJETQUANTI s 0 VALUE BIOJETQUANTI s 0 VALUE TANDEM CAVROS 0 VALUE TANDEM BJQ s 2 VALUE TANDEM AJQ s 1 VALUE PIN ARRAYS 0 VALUE EDIT AIRJET EDIT AIRJETQUANTI EDIT CAVRO e EDIT AIRJETQUANTI EDIT BIOJETQUANTIS EDIT BIOJETQUANTI EDIT TANDEM CAVROe EDIT TANDEM BJQ e EDIT TANDEM AJQ EDIT PIN ARRAY e EDIT TANDEM CAVRO BIOJET DRIVERS 1 VALUE BJ PROFILE NPB STD STROBE TIME ms 0 VALUE STROBE OUTPUT 65535 VALUE TAKEUP NAME TAKEUP TENSION MOTOR ROTATION INTERIOR EXTERIOR ENCODER 0 CENTER 47 SET CENTER e HOME STATE ON OFF TCM ENABLED DISABLED TYPE TAKE UP PAYOUT CALIBRATE ACC mm s2 1000 VALUE RATE 0 0200 VALUE GAIN 1 0000 VALUE INTERVAL ms 50 VALUE DEBUG DISABLED ENABLED PUT MATERIAL IN PRESS lt ENTER gt lt ABORT gt TO EXIT CENTER THE DANCER PRESS lt ENTER gt lt ESC gt TO EXIT ENCODER 0 000000 TENSION MOTOR NAME TAKEUP 104 BUS CPU BOARD ADDRESS 772 VALUE UNITS m
28. 11 500 00 0 83 1 28 2 00 83 33 0 42 0 75 7 00 250 00 0 36 1 11 125 00 0 63 0 85 333 33 0 48 1 19 166 67 0 83 0 93 500 00 0 71 1 29 2 25 83 33 0 37 0 74 8 00 333 33 0 42 1 17 125 00 0 50 0 77 500 00 0 63 1 25 250 00 1 11 1 11 666 67 0 83 1 37 2 50 83 30 0 33 0 73 10 00 500 00 0 50 1 30 125 00 0 50 0 85 666 67 0 67 1 33 166 67 0 67 0 95 1000 00 1 00 3 00 2 75 83 30 0 30 0 76 15 00 625 00 0 42 1 33 125 00 0 45 0 85 1000 00 0 67 3 07 250 00 0 90 1 11 3 00 125 00 0 42 0 83 20 00 1000 00 0 50 3 00 166 67 0 56 0 94 250 00 0 83 1 10 3 50 125 00 0 36 0 86 25 00 1000 00 0 40 2 40 166 67 0 48 0 95 250 00 0 71 1 10 BIODOT Operating Manual Version 1 5 Appendix C Dispense Optional Components Page 64 APPENDIX C DISPENSER OPTIONAL COMPONENTS Page 65 LINE PRESENCE INSPECTION SYSTEM The Line Presence Inspection LPI system is an option available for BioDot dispensing systems to detect the presence or absence of a dispensed line This system utilizes fiber optic technology that includes a reflectance sensor fiber optic amplifier and ink jet marker The sensor inspects for absence of the dispensed line and marks the web material when the line dot is missing The LPI system components should be installed with the help of a BioDot technician proper placement of the fiber optic cables and accurate system calibration is required for the system to perform correctly We have provided a very basic description of the system and th
29. 31 VALUE ON TIME ms 0 8 VALUE TRANS 1 ms 75 VALVE TRANS 2 ms 75 VALVE TANDEM BJQ 1 ACTIVE NO YES RATE uL cm 1 00 VALVE VOL nL 31 0 VALUE PITCH mm 0 31 VALUE ON TIME ms 0 6 VALUE TRANS 1 ms 75 VALVE TRANS 2 ms 75 VALVE BioDot Dispensers PRIME DISPENSER TANDEM BJQ AJQ DEVICE 1 VALUE ACTIVATE GROUP NO YES ACTION PRIME EMPTY SYSTEM UTILS CAPSTAN TRACKING MARKERe STATISTICSe MEANS THERE IS NO VALUE CONFIGURATION s DATA STORAGE CHECK MEMORY SYSTEM INFO e WATCH INPUTS amp PRIME DISPENSER TANDEM AJQ BJQ ACTIVATE ACTION PRIME EMPTY I INPUTS 1 ACTIVE 012345678 000001000 HIT ESC TO EXIT STATISTICS 31 852 HRS 1405 560 M CLEAR MACHINE TIME CLEAR TOTAL LENGTHe SYSTEM INFO SERIAL XXXX BIOS V4 00 FIRMWARE V1 211 BD LIB V1 33 KERNEL V1 21 AXIS LIB V1 35 DISP LIB V1 42 E MEMORY AVAILABLE CURRENT 146446 PRIME MARKER amp PRIMING MARKER PRESS ESC TO ABORT CAPSTAN TRACKING TCM PAYOUT TAKEUP DRIVE CAPSTAN ON OFF DIP TANK OFF ON DISP TRACK OFF ON TAKEUP TRACK OFF ON puc oc DATA STORAGE CLEAR TOTAL LENGTH SAVE DATA e PRESS GOTO CONFI
30. AT Z in ZX platform REPEAT 1 These options RATE uL cm UNDER AIRJETQUANTI 1 REPEAT 2 not available REPEAT Z in ZX platform Operating Manual Version 1 5 BioDot Dispensers B Page 34 EDIT Sub Menu Options Defined Reel to Reel Platform Only NAME This is the name of the current program selected program under the SELECT menu To change the name simply enter a new name and press ENTER TANDEM n There is n of these menu items for each dispenser for which the unit is configured where the number nis the dispenser number from left to right when facing the platform Submenus appear when a dispenser is selected and a checkmark appears after the name of the dispenser in the TANDEM 7 field The operator enters the dispense cycle parameters for each dispenser in the submenus Edit Sub Menus ACTIVE RATE Same as for XYZ ZX platform see below for definitions VOL PITCH ON TIME TRANS This field allows you to program the transition period in milliseconds between the end of one syringe pump stroke and the beginning of the other XYZ and ZX Platforms EDIT SUB MENU FIXED OPTIONS NAME This field displays the names of all saved programs The operator selects a program to edit by using the arrow keys to scroll through the options and select the desired program and the ENTER key to activate ACTIVE This is a toggle field YES NO that
31. MAINTENANCE OF 65 44 44424445 u u u u 47 B ckground a 47 Key Words n eee errs 47 Eole d oela EIA A A 47 Procedure Cleaning and Maintenance of BioJet AirJet and Frontline Dispensers 47 BENNAE 221410 E 47 Weekly cleaning and Decontamination Standard Procedure 48 Weekly cleaning and Decontamination Alternative for Systems With a Narrow pH Tolerance 48 UNnCIOQGING Procedure uns na 49 Procedure for AirJet assembly nn 49 ZU Je 3000 ASSCMDIV oleo ND UEM 53 APPENDIX A TROUBLESHOOTING iri 56 BioJet Dispenser Troubleshooting Guide a eee 57 AirJet Dispenser Troubleshooting Guide sss 59 BIO DOT BioDot Dispenser Operating Manual Version 1 5 Table of Contents iv APPENDIX BIOJET DISPENSER RATE TABLES
32. ON Background In this chapter you will learn how to design input and store dispensing programs for BioDot system platforms using the hand held terminal For the sake of continuity we will focus on the programming capabilities of the hand held terminal relevant to the dispensers a more in depth discussion of platform configuration with the hand held terminal can be found in the system platform manual We suggest that you go through this section of the manual with the hand held terminal in hand This is useful for two reasons First it will help you become familiar comfortable with the terminal itself Second as there are some differences in the menu structure between platforms you might find it useful to note specifics of your menu programming in the manual as you go along F The goal of this chapter is to instruct you in Running a Dispense Cycle and Inputting Programs with the platform hand held terminal and to explain the operational aspects of the individual dispenser types that impact the design of dispensing patterns Key Words LCD Screen Display The Liquid Crystal Display LCD is the 4 line screen located on the hand held terminal Non volatile Memory Non volatile Memory is memory that keeps its data when power is lost to the unit Non volatile memory is used for long term storage of data Working Memory Working Memory is used to create or modify data Data in working memory are lost when the unit is powered down unless saved under
33. OQramms E LE 30 BioJet Q antr 3000 Op r ati ni u A SSS 36 PERPE 36 Programming Considerations t in e ba o be b ri eb o a e E Ee ba E Ea E EE Ea RR ERR ERE 36 BioDot Dispensers B Operating Manual Version 1 5 Table of Contents iii Eine DISPENSING ss idi u Db T 37 Dot DISPENSING aaa uassabaqaashaasqasshawsasqhawsqawqhasshasshasalasqhassasqhaqsqass awahuqqlassqawqhaqaqayshawaslauqlassqawahaqaqayahawaqaqqhaysqasahaqqqasaayaqayqqassqawaqawaqdaaqaaq 37 TNE DISDONS Cl osos Sua AO A NA ANA AANA A E 37 Contiguring vs 38 AirJet Quanti 3000 2300 and 2400 i 39 Frontline Quanti Operador tette tse t ua eee 41 Syringe Pump Operation ARR 41 Test Programs for BioJet Dispensers 42 ias 86 42 Dot Dispense S 43 Dispense 44 Remedial 45 CHAPTER FOUR CLEANING AND
34. REPEAT 2 0 In Dispenser Sub Menu Leak Test Instructions Using the Prime Dispenser command on the handheld controller prime the BioJet with 0 05 BioTerge Observe the dispense stream The dispense should be in the form of a straight stream of fluid without splashing or sputtering If splashing or sputtering occurs during dispensing perform cleaning as outlined in Maintenance and Cleaning of the BioJet Quanti 3000 Wipe the tip clean before each run is performed Examine the tip of the BioJet valve after the program has run Determine if a meniscus of fluid has formed at the tip or if a droplet has fallen free The valve passes i e no leak is occurring if no fluid has been forced through the valve If a droplet is seen on the tip a leak has occurred To ensure that the leak has not occurred from the inlet tubing to the valve remove the valve from the housing leaving the electrical connector attached to the valve Wipe the entire valve clean of any fluid Repeat the test watching the inlet tubing specifically as a source of the fluid leak If no fluid leaks from the inlet line the valve itself has leaked The Dot Dispense Test which tests for consistency in dot dispensing should be applied next Dot Dispense Test This program is designed to determine if the BioJets are capable of dispensing a consistent drop of fluid under optimal dispensing conditions To do this the user will pr
35. RESS 3 TAKEUP TRACKING DRIVE CAPSTAN e OUTPUT 4 VALUE ALARM e INDEX SENSOR DIP TANK PRESS 1 ERRORS SENSORS INSP SENSORS e INSPECT ms 0 VALUE DM 5604 OUTPUT 65535 VALUE DIP TANK PRESS 2 EXTERNAL STATUS TIMEOUT ms 0 VALUE REMOTE GO OUTPUT 65535 VALUE DIP TANK PRESS 3 OUTPUT 65535 VALUE PUT MATERIAL IN PRESS lt ENTER gt lt ABORT gt TO EXIT CENTER THE DANCER PRESS lt ENTER gt lt ESC gt TO EXIT ENCODER 1 000000 AUX DISPENSE DRIVE CAPSTAN BioDot Reel To Reel Menu Tree OUTPUT 6 VALUE ALARM OUTPUT 65535 VALUE INDEX SENSOR INPUT 65535 VALUE ACTIVE STATE ON OFF ERROR SENSORS SENSORS 0 VALUE INSP SENSORS SENSORS 1 VALUE SENSOR 1 DM 5604 ADDRESS 0 VALUE EXTERNAL STATUS INPUT 65535 VALUE ACTIVE STATE ON OFF REMOTE GO OUTPUT 65535 VALUE AUX DISPENSE OUTPUT 65535 VALUE SENSOR 1 SENSOR ON OFF OFFSET mm 53 5 VALUE INSP SENSOR E INSP SENSOR INPUT 5 VALUE ACTIVE STATE OFF ON TENSION MOTOR NAME PAYOUT 104 BUS CPU BOARD ADDRESS 768 VALUE UNITS mm MOTOR STEPS 7200 VALUE uSTEPS 64 VALUE TRAVEL 500 0
36. RM OR ABORT CANCEL ER L LOADING DATA DONE CLEAR MACHINE TIME PRESS GO TO CONFIRM TO CANCEL STORING DATA DONE MARKER CONFIGURATION EDIT AXESe DIR FORWARD REVERSE LOW ERROR OFF EDIT DISPENSERe EDIT TCM s PRIME uL s 50 0 VALUE FILL uL s 50 0 VALUE BAD MARKER FREQ hz 10 VALUE ON TIME 2 0 VALUE MARKER TPU 7 VALUE MARKER OUTPUTe HOME mm sec 40 VALUE TIMEOUT ms 5000 VALUE TYPE REEL TO REEL LAMINATOR R TO R TYPE CONT INDEX BOTH Y AXIS NO YES OUTPUT 7 VALUE EDIT TCM s TOM S 2 VALUE PAYOUT e TAKEUP MARKER OUTPUT Z CYLINDER OUTPUT 65535 VALUE ANTI STATIC UNIT Z CYLINDER ANTI STATIC UIT LINEAR TAKE UPS OUTPUT 65535 VALUE LINEAR TAKE UPS DISPENSE TRACKING TAKEUP TRACKING DIP TANK PRESS DIP TANK PRESS 2 OUTPUT 65535 VALUE DISPENSE TRACKING OUTPUT 5 VALUE PAYOUT NAME PAYOUT TENSION MOTOR ROTATION INTERIOR EXTERIOR ENCODER 1 CENTER 85 SET CENTER HOME STATE ON OFF TCM ENABLED DISABLED TYPE PAYOUT TAKEUP CALIBRATE e ACC mm s2 1000 VALUE RATE 0 0200 VALUE GAIN 1 0000 VALUE INTERVAL ms 50 VALUE DEBUG DISABLED ENABLED DIP TANK P
37. Three Dispenser Operation for dispensing instructions 9 Note We have not included specific testing protocols for the AirJet dispenser as it is compared to the BioJet a far less complex dispenser to set up and use See the Airjet troubleshooting guide if problems arise BIODOT BioDot Dispensers Operating Manual Version 1 5 Page 17 Frontline Dispenser Installation Unpacking 9 1 Remove the dispenser s from the packing container and carefully remove the dispenser from its plastic wrapping Note The dispensers may be already attached to the head assembly Remove the head assembly from the protective box and install on the platform with the supplied hardware A Warning Frontline Dispenser tips are fragile and should be handled with care 2 Locate the packing checklist that is provided with the instrument Please go over this list to confirm that all ordered parts tools and accessories are accounted for an item is missing please see Support Service in Appendix D of this manual Installation The frontline dispenser is very easy to set up and use The dispenser simply attaches to the vertical positioning stage and is 27 secured by a screw Adjust the position of the dispenser as described below Note The Frontline dispenser may be facing in either direction depending on the application and platform it is being used on Ensure system power is OFF Install the Syringe Pump see Syringe Pump Installation Sec
38. anual 9 Note Syringe Pumps are fully assembled on arrival and may already be attached to the platform depending on the unit Please refer to Figure 9 Refer to Figure 4 for pump definition You may need to remove the blue pump valve cover and attach the bottle clip with the supplied hardware BIODOT BioDot Dispensers Operating Manual Version 1 5 Page 19 A Warninq Please DO NOT attempt to take a Syringe Pump apart without first contacting BioDot Support Service DO NOT remove any cable with the power ON Attempting to disassemble a dispenser could damage the instrument and will void your product warranty Cable Assembly Connector to Syringe Pump Dispenser Line 15 pin Connector Syringe Pump To Dispenser or Material Directly 3 Port Valve Syringe Pump Cable Assembly Supply Lines From Bottles Pump Cable Harness Power Cord Receptacle Bottle Holder Standard Pump Syringe Pump Syringe Power Connection Syringe Pump Cables AMP Connector d Supply Bottles and Jack Com Cable Syringe Pump to Platform Platform Cap Screws Figure 9 Installation The syringe pumps require proper connection to the system platform central processing unit CPU via the cable assembly COM line or harness proper connection to system platform power via the cable assembly AMP connector and proper connection to each other via the cable assembly connectors one for each standar
39. by Q removing some of the variables of standard operation Sample test programs are outlined for both old and new format programming Old Board and New Board Note None for Old first generation boards The user should employ these test programs in conjunction with the protocols described in Cleaning and Maintenance of Dispensers in attempting to remedy a problem and in determining whether replacement of a valve is warranted Test Program Goals In determining correct operation of the valve the operations to be observed are e The ability of the valve to prime correctly e The integrity of the valve i e does it leak e The ability of the valve to deliver a consistent dot e The ability of the valve to dispense a consistent line Leak Test This program is designed to determine if the valve is leaking The aim is to activate the dispenser but not open the valve thereby determining whether fluid can be forced through a nominally closed valve This sample program is written for XYZ or ZX platforms but may easily be adapted for other platforms as well The Leak Test program is outlined below BioDot Dispensers B I Operating Manual Version 1 5 Page 43 NAME Leak Test ACTIVE Yes DISPENSER BioJet Quanti 1 SHAPE Dot Z ENABLED Yes X START mm 0 Y START mm 0 DROP nl 100 TOTAL nl 100 DROPS 1 FREQ Hz 100 ON TIME ms 0 REPEAT 1 25 X OFFSET mm 0 Y OFFSET mm 0
40. d pump Please refer to Figure 9 while going through the installation procedure Note If you are installing BioJet Plus pumps please refer to the BioDot OEM pump manual for details BioDot Dispensers B I D O T Operating Manual Version 1 5 Page 20 1 If pumps require mounting Locate the two pre drilled attach points for the syringe pump s on your system platform XYZ or ZX platform or pump platform Reel to Reel platform 2 Attach the syringe pump s to the system platform or pump platform using the two No 6 32 x 1 2 inch long socket head cap screws provided 3 Once pumps are mounted to platform Connect the cable assembly connector to the syringe pumps rear 15 pin connector standard pump Secure the connector with the screws in the connector housing Repeat this process for all of the syringe pumps 4 Connect the 9 pin serial interface cable labeled COM from the cable assembly to the platform connector labeled 5 Locate the AMP connector for the syringe pump s Insert the connector into the corresponding connection on the rear of the module J1 J1 etc 9 Note There may be some variation in labeling of plugs and jacks see also system platform manual for further details 6 Fita syringe of appropriate size to the pump e Insert plunger all the way inside the syringe barrel prior to installation e Screw the syringe barrel into the receptacle in the pump valve assembly Loosen the plunge
41. dard length in the X direction is 370 mm 500mm optional The maximum length in the Y direction is 70 mm for the XYZ3000 and 265 mm for the XYZ3200 POLARIZATION This is a toggle action YES NO that forces a line to be drawn in only one direction when turned to ON To BioDot Dispensers BIODOT Operating Manual Version 1 5 Page 35 speed dispensing of multiple lines select NO SPEED mm sec The field allows you to program the rate at which the platform will move during the line dispense Typical values are 50 and 100 mm s Speeds over 100 mm sec are rarely necessary or appropriate and are not recommended ACC mm s The Acceleration ACC is the rate at which the platform will accelerate up to the desired speed Acceptable values range from 12 mm sec to 96 297 mm sec The operator can adjust this rate as necessary At some platform speeds a high acceleration value may stall the motors Typical acceleration values are around 1 000 mm s PITCH mm This field allows you to program the dot pitch and is displayed for the LINE task only X and Y start positions are used for the DOT task Please refer to the BioJet Dispenser Rate Tables in Appendix B for appropriate rate settings VOLUME ul cm This field allows you to program the volume of liquid dispensed per unit length expressed in uL cm and applies when the SHAPE menu has been toggled to the LINE option RATE
42. e SHAPE LINE or DOT to be performed Please see Inputting Programs for information on entering your desired program To design a line dispensing program we suggest you start with the RATE DROP VOLUME DROP PITCH and ON TIME values that are appropriate for your application Use the rate tables in Appendix B as a reference to your dispense parameters Once these four interdependent program parameters have been selected you can then build on to your dispensing program using the remaining EDIT sub menu option such as line length angle platform speed etc Dot Dispensing As mentioned earlier dot dispensing can be accomplished two ways by using the DOT or the LINE task To use the LINE task for dot dispensing simply select LINE for your SHAPE option under the EDIT sub menu Then input a line dispensing program with an expanded DROP PITCH To use the DOT task for dot dispensing simply select DOT for your SHAPE option under the EDIT sub menu and input a dot dispensing program See Inputting Programs and The Edit Sub Menu for details Priming the Dispenser A dispenser should be primed in preparation for both dispensing and cleaning You will use the PRIME DISPENSER menu to perform the priming sequence The priming speed is selected under the CONFIGURATION menu See REPEATS and PRIME BioDot Dispensers B I Operating Manual Version 1 5 Page 38 DISPENSER the Accessory Dispensing Menus and PROGRAM and CONFIGURATION The Disp
43. e Solutions General Tips Ensure that the proper dispenser has been selected in the Program Ensure that all reagent lines are connected securely and are free of clogs and leaks Ensure that the proper volume is entered in the Program Programmed volume should not be less than 1 ul cm Ensure that the dispenser has been properly primed Ensure that the appropriate syringe size has been configured Ensure that the airlines are connected properly and the appropriate amount of pressure is applied to the AirJet Ensure the micrometer atop the AirJet is open sufficiently Ensure that the dispense head is at an appropriate distance from the surface of the substrate approx 1 3 cm Ensure that the air pressure is set appropriately The AirJet is commonly operated at 8 10psi Ensure that the transition times Trans 1 and 2 are set correctly for the fluid you are dispensing These settings are determined empirically and may differ for each fluid and tandem pump set Having these settings correct will ensure that no gaps or bulges in dispensed lines will occur upon pump transition Breaks in lines Check air pressure height of dispense head above substrate and opening of micrometer Generally air pressure is operated at 8 10 psi and the micrometer is set at 1 3 for commonly dispensed fluids such as gold conjugates or blocking reagents Alter one parameter at a time until line quality improves BioDot D
44. e Syringe Pump Installation 3 Attach the BioJet support arm to the Vertical Positioning Platform in some systems the support arm will be attached to the system platform at the factory and may attach and look differently using the supplied hardware See Figure 5 4 Mount the BioJet Quanti Dispenser s via the small mounting bracket to the support arm using the supplied hardware see Figure 5 5 Obtain the coaxial cable s provided for dispenser hook up Standard pumps 6 Connect the coaxial cable to the BNC connector marked BioJet 1 see Figure 6 e Push the cable connector onto the BNC connector to engage Turn the cable connector turn clockwise to lock the connection in place Repeat for additional BioJets BioDot Dispensers Horizontal Y Axis Positioning Stage with Micrometer Only On Some Units Vertical Z Axis Positioning Stage with Micrometer Figure 5 BIODOT Page 13 Operating Manual Version 1 5 Page 14 Barbed Fittings With Locking Sleeves Other End Of BioJet Power Cable BNC Connectors 4 shown BioJet Plus End BioJet Power Coaxial Cable Adjustable Slide Bracket Figure 6 9 Note Where multiple dispensers are installed the first dispenser must be connected to the connector labeled BioJet 1 the second one to BioJet 2 etc Their order BioJet 1 BioJet 2 etc corresponds to their position on the platform from left to right where the user i
45. e hole goes all the way through the spindle so you have 2 turn tolerance Refer to photo Line This Hole With Hole In Spindle Hole Inline With Hole Body In Body e Assemble the jam nut to the spindle threads and tighten in place Use the spanner wrench supplied with the micrometer item 12 to accomplish this BioDot Dispensers B l D O I Operating Manual Version 1 5 Page 55 e Insert the four setscrews item 19 into the needle head item 8 and allowing tip of setscrew to remain slightly above flush with the outside of the needle head e Trial fit needle head into rear of spindle so that it is fully seated Slide the needle item 6 through the rear of the needle head until it is exposed at the front of the nozzle At this point the rear of the needle should be below flush with the rear of the needle head A B Warning Be extremely careful not to damage needle tip as it can be easily damaged If resistance to insertion is found stop refer to Stop above and reexamine cause before proceeding e While holding the needle head against the spindle and holding the needle fully seated against the nozzle tighten all four setscrews a like amount and secure needle in place e Remove assembled needle and needle head and reassemble with spring item 17 in place Assemble needle cap item 1 to front of AirJet body to protect needle when fully extended e Assemble micrometer housing item 9 to expose spindle threads and tighten b
46. e installation process below Please contact BioDot Support Service with questions or to make adjustments to the LPI system Background The LPI system consists of three main components as shown in Figure 11 on the following page Fiber Optic Wires Transmit Receive Side Wire Transmit Side Wire A wire identified by the presence of colored dots every 12 inch which carries a fiber optic beam of light to the web material Receive Side Wire A solid black cable which carries reflected light back to the system Amplifier Amplifier A sensor amplifier which monitors measures and translates the reflected light intensity Inkjet Marker The InkJet Marker for the system is a BioJet dispenser modified for the inspection marking function Tools and Equipment e Allen wrench set standard BioDot Dispensers B I Operating Manual Version 1 5 Page 66 Direction Of Flow Transmit Side Fiber Optic Wires Dots Inkjet Marker F V22f Dispenser Receive Side Fiber Optic Wires Solid Black Line Presence Inspection System Sensor Line Presence Inspection System Amplifiers Figure 11 B Operating Version 1 5 BioDot Dispensers Page 67 Basic Installation 1 Ensure platform power is OFF 2 Install your BioJet Dispenser s per Chapter Two Dispenser Installation 27 Note If an LPI system is included in your dispensing system your BioJet
47. embly Procedure 9 Note All item numbers reference the drawing sheet below Use tools supplied by manufacturer Q PAR O RIP TIO 0013 TIP CROWN AIRBRUSH 3MM 0005 CAP NOZZLE AIRBRUSH 3MM 0009 NOZZLE AIRBRUSH 3MM 0304 SPINDLE AJ3000 0305 BODY AJ3000 0306 NEEDLE AJ3000 0307 SCREW GLAND AJ3000 E 0308 NEEDLEHEAD AJ3000 8 FA 0309 HOUSING MICROMETER ab D gt 1 I 6004 0310 NUT JAM AJ3000 Z 6004 0311 SHIM AJ3000 6 C 12 1 3018 0000 MICROMETER HEAD 50 TRAVEL T pem 3020 0008 O RING AS568A 003 56 ID X 060 W EPDM 40 14 2 3020 0009 O RING AS568A 005 101 ID X 070 W EPDM 15 1 3020 0005 O RING 239 ID X 025 W EPDM 16 1 3020 0002 O RING 055 ID X 016 W EPDM 17 1 3037 0000 SPRING COMPRESSION 180 X 024 WIRE 18 1 4001 0039 FITTING MALE ADAPTER 1 4 TUBE OD 10 32 THD 19 4 3030 0050 00 SCREW SET CUP POINT 0 80 X 1 8 LONG e Assemble tube connector item 18 to the AirJet body item 5 and tighten securely Place o ring item 15 onto thread undercut of AirJet body making sure that there are nicks or blemishes on the o ring surface which may cause it to leak BioDot Dispensers B Operating Version 1 5 Page 54 e Assemble the nozzle cap item 2 to the AirJet body and tighten firmly by hand Temp
48. enser Programming Menus for details on priming the BioJet These sections are found in the Inputting Programs section of this chapter Configuring the Syringe Pumps See Syringe Pump Operation below BioDot Dispensers B 1 T Operating Manual Version 1 5 AirJet Quanti 3000 2300 and 2400 Operation General Operation Page 39 The AirJet Quanti dispenser essentially operates by injecting a metered supply of reagent into a flowing air stream Reagent is then aspirated onto the web material Flow rate is controlled by the syringe pump and by adjustment of the needle micrometer on the AirJet The AirJet is capable of dispensing lines or dots with a wide range of dispensing rates and line widths see Chapter One Dispenser Specifications A cross sectional view of the end of the AirJet is shown in Figure 10 The fluid path opens into an orifice at the AirJet tip that is formed by the needle and nozzle Adjusting the needle position in the vertical direction using the needle micrometer controls the size of the fluid orifice and thus the rate of flow from the AirJet BioDot Dispensers Air Path Nozzle Cap Nozzle Dispensing AirJet Ti Orifice Needle Figure 10 Operating Manual Version 1 5 Page 40 Control of Line Width and Volume The needle micrometer on the top of each AirJet is the primary control over the dispensed volume Turning the micrometer towards a larger numb
49. er on the micrometer dial counter clockwise turn when looking down on the micrometer backs the needle out of the nozzle resulting in a larger fluid orifice and corresponding increase in the line width The dispense rate is not affected by the needle position but the shape and quality is extremely sensitive to small changes in needle position A Warning Although the AirJet has been equipped with a positive stopping device to prevent needle nozzle damage due to overdriving the micrometer it is still necessary to refrain from using excessive force when closing the fluid orifice Overdriving the micrometer could seriously damage the AirJet The vertical micrometer stage on each AirJet mounting platform is the primary control of line width Spraying close to the material will result in a finer line When operating extremely close to the material it may be necessary to switch to the crown tip which will allow the air stream to escape from the dispensing area through the cutouts in the tip The crown tip is supplied in the tool kit and replaces the standard AirJet tip Z UP and Z Down Feature in AirJet Dispensing For units with a pneumatic Z Axis the Z DOWN feature is used to delay premature dispensing of the AirJet An increase in time on the Z DOWN will allow the head to come down and wait before dispensing This is useful in cases where you have a low air supply or worn components in the Z Axis assembly The Z UP feature happens at the end o
50. er problems from static electricity than nitrocellulose for example Examine the lines for consistency for satellite drops or spray or for breaks BioDot Dispensers B I Operating Manual Version 1 5 Page 45 The Line Dispense Test program is outlined below NAME Line Dispense Test ACTIVE Yes DISPENSER BioJet Quanti 1 SHAPE Line Z ENABLED Yes LENGTH mm 300 ANGLE deg 0 POLARIZATION No SPEED mm s 50 ACC mm s2 1000 X START mm 10 Y START mm 10 DROP nl 20 83 PITCH mm 21 VOLUME 1 0 ON TIME 0 2 1 2 X OFFSET Y OFFSET REPEAT 2 0 2 Dispenser Sub Menu 0 5 Line Dispense Test Instructions Prime the dispenser prior to performing the test Determine whether satellite drops are present or there is sputtering from the valve or significant breaks in the dispensed line that are not attributable to air in the fluid lines The valve passes if none of the above is present Remedial Actions If the valve fails any of the above tests undertake cleaning as outlined in Cleaning and Maintenance of Dispensers and retest the valve for performance Once all cleaning procedures including the unclogging procedures outlined in that protocol have been performed retest the valve for performance as above If the valve passes the inspections it may be cons
51. f the dispense cycle and the Z Axis is in the up position An increase in time on the Z UP will allow the user time to reposition or remove a substrate For multiple cycles the Z UP allows time for the previous pattern to set before the second pattern is applied 22 Note Delays are user and application defined If you feel you need more time then program more time If less then program less The viscosity of the solution the rate that you are applying it and the substrate being used may also be determining factors on how long or short you program delays It may take some trial and error to determine suitable delay times BioDot Dispensers B 1 T Operating Manual Version 1 5 Page 41 Line Dispensing To design a line dispensing program for the AirJet we suggest you start with the RATE as a basis for your program A line dispensing program for the AirJet is entered using the EDIT sub menu under the PROGRAM Reel to Reel or PATTERN XYZ or ZX menu You will need to create a program NAME select the DISPENSER or DEVICE to be used and select the SHAPE LINE or DOT to be performed Please see Inputting Programs for information on entering your desired program Dot Dispensing Dot dispensing with the AirJet on an XYZ platform can be accomplished by using the DOT task XYZ Platforms only To use the DOT task for dot dispensing simply select DOT for your SHAPE option under the EDIT sub menu and input a dot dispensing program See In
52. gent Q The following syringe pump sizes are available 50 ul 100 ul 250 ul 500 ul and 1 0 ml Note A crown tip is supplied in the toolkit that comes with each AirJet The crown tip is an alternative tip that should be used when dispensing very close to the web material when a finer line is required Refer to Figure 2 AirJet Quanti 3000 Specifications The AirJet Quanti 3000 is the smallest aerosol dispenser in this series with a nozzle needle size of 0 30 mm Power Air Requirements Power is derived from a BioDot dispensing platform 110 220 VAC 50 60 Hz Air source required 10 40 PSIG 0 70 2 80 Kg cm it must be a clean dry and filtered air source at constant pressure Dispensing Parameters Dispensing Range 1 ul cm 22 ul cm dependent on fluid rheology Line Width Range 0 02 inches 0 5 mm to 0 24 inches 6 mm line width is highly dependent on fluid rheology and substrate properties Repeatability 1 Line dispensing Swept Dead Volume approximately 1 5 ul AirJet Quanti 2300 Specifications The AirJet Quanti 2300 is the second largest aerosol dispenser in this series with a nozzle needle size of 0 60 mm Power Air Requirements Power is derived from a BioDot dispensing platform 110 220 VAC 50 60 Hz Air source required 10 40 PSIG 0 70 2 80 Kg cm it must be a clean dry and filtered air source at constant pressure Dispensing Parameters Dispensing Range 1 ul cm lt 80 ul cm de
53. gent 2 Clean and refill the supply bottle with deionized water containing 0 05 BioTerge to enhance scrubbing of interior recesses within the BioJet Quanti BioDot Dispensers B Operating Manual Version 1 5 Page 48 3 Prime dispenser for 5 syringe cycles 4 Repeat steps 1 through 3 with deionized water Weekly cleaning and Decontamination Standard Procedure After prolonged dispensing of reagents some buildup of protein constituents salts latex materials or other particulate matter may occur It is recommended that the following cleaning steps be followed on a weekly basis to dissolve any accumulated materials This should be performed in addition to the daily cleaning procedure 1 Purge supply lines of reagent 2 Clean and refill the supply reservoir with a dilute base 0 1N NaOH Prime the BioJet Quanti for 5 syringe cycles and allow to sit for 10 minutes 3 Prime using deionized water as in steps 1 and 2 above 4 Clean and refill the supply reservoir with a dilute acid 0 1N HCl Prime the BioJet Quanti for 5 syringe cycles and allow to sit for 10 minutes 5 Purge the supply lines and prime for a minimum of 10 cycles using deionized water Weekly cleaning and Decontamination Alternative for Systems With a Narrow pH Tolerance This procedure should be performed in systems dispensing proteinaceous reagents or where acid base use is undesirable This procedure may also be performed as part of
54. get If possible work in relative humidity of gt 30 when striping nitrocellulose in particular Humidify the area if necessary Line quality is generally poor Thick lines breaks in lines poor absorption into the membrane can all be caused hydrophobic patches in the membrane due to poor quality membrane being used or membrane being stored for too long or in less than optimal conditions Try a different membrane for comparison purposes BioJet Valve is suspected of dispensing incorrect volume Check the volume dispensed using the Test Programs for BioJet Dispensers in Chapter Four of the manual Diagnosing the BioJet valve Check for visual signs of damage to the valve Verify the coil resistance is correct A If resistance is infinite then the coil winding has broken most likely due to over powering voltage or overheating ambient cooling is too low B If resistance is too low then the coil winding has broken or insulation on coil winding has melted and the electrical flow path is not through the whole coil Check for contamination internal to the valve which will cause leakage or no flow by pressurizing the inlet port with air and routing the outlet port under water via tubing No visible signs of air bubbles dispensing from the outlet of the tubing should be present BioDot Dispensers B 1 O D O T Operating Manual Version 1 5 Page 59 AirJet Dispenser Troubleshooting Guide Problem Possibl
55. h a 250 ul syringe at full stroke depends on how where lines have been cut Volume Recovery 95 The BioJet Quanti Dispenser is illustrated on the following page BioJet Quanti Dispenser Illustrated Three BioJet Quanti dispensers with associated components are shown in Figure 1 These figures are representative of the 3000 model and are shown here in the context of a BioDot system platform with vertical and horizontal positioning stages Please take a moment to familiarize yourself with the basic layout of the dispenser before proceeding to Chapter Two Dispenser Installation Note The barbed inlet port may have a BioJet Plus fitting instead The supply line will have to have a matching fitting BIODOT BioDot Dispensers Operating Manual Version 1 5 Page 5 BioJet Power Cable Supply Line Connection Point LPIS Sensor BioJet Barbed Inlet Port LPIS Fiber Optic Cable Locking Sleeve Support Arm With Adjustable Slide Positions BioJet Plus Fitting Line Presence Inspection System Optional Component see App LPIS Amplifier Vertical Z Axis Positioning Stage With Micrometer Barbed Fitting BioJet Tip With Guard BioDot Dispensers B I T Operating Manual Version 1 5 Page 6 AirJet Quanti Dispensers This series of dispensers couples aerosol spray airstream technology with one or two syringe pumps to meter precise amounts of rea
56. id valve in the dispenser For continuous dispensing operations two pumps are operated in tandem The two pumps signal each other at a given time the transition time before the end of their stroke so that the other can begin its stroke at the appropriate time thereby maintaining a constant pressure in the tubing that feeds the BioJet The BioJet can be programmed to deliver distinct drops composed of one or more of these stepper increments or streams of droplets to form lines Programming Considerations The basic difference between a line and a series of dots is simply the drop pitch Using the hand held terminal the operator programs a drop pitch that will increase or decrease the density of drops in a line For every reagent type there will be a minimum drop density that is necessary to obtain a continuous line BioDot Dispensers B I T Operating Manual Version 1 5 Page 37 2 Note There must also some consideration of protein binding the choice of minimum drop density If the protein binding is extremely fast then the actual outline of the drop can be seen giving a scallop effect to the edge of the line Using smaller drops and a higher drop density to achieve the desired line concentration can minimize this For high drop densities the reagent can puddle on the membrane surface This can result in splashing effect as subsequent drops impact the liquid Using lower table speeds with the same drop density al
57. ide Block Locking Screw Adjustable Mounting T Bracket Tip Support Dispense Tip Figure 3 BioDot Dispensers B I D D T Operating Manual Version 1 5 Page 10 Syrinqe Pump Dispenser The Syringe Pump Dispenser is the driving force behind BioJet Airjet or Frontline Quanti Dispensers though it may be used on its own to dispense precise amounts of fluid The syringe pump dispenser utilizes a positive displacement pump a programmable stepper motor and a syringe The following syringe sizes are available 50 ul 100 ul 250 ul 500 ul and 1 0 ml Power Air Requirements Power is derived from a BioDot dispensing platform 110 220 VAC 50 60 Hz this dispenser does not utilize air Dispensing Parameters Pump literature is provided with the unit The Syringe Pump Dispenser Illustrated A pair of syringe pump dispensers with associated components is shown in Figure 4 below Please take a moment to familiarize yourself with the basic layout of the dispenser before proceeding to Chapter Two Dispenser Installation Dispenser Line Syringe Pump To Dispenser or Material Directly 3 Port Valve Supply Lines From Bottles Pump Cable Harness Bottle Holder Standard Pump Syringe Supply Bottles Standard BioJet Plus Platform Figure 4 BIODOT BioDot Dispensers Operating Manual Version 1 5 Page 11 CHAPTER TWO DISPENSER INSTALLATION Background BioDot dis
58. idered suitable for continued use If the valve does not pass the above inspections it may be concluded that the valve needs to be replaced BioDot Dispensers B T Operating Manual Version 1 5 Page 46 If the valve passes the above tests and inconsistencies are being observed in the dispense of your fluid of interest onto its substrate then it is likely that factors other than valve performance are involved Factors which can lead to inconsistent dispenses include e Particulates in the fluid either present intentionally or as artifacts If possible filter fluids prior to dispensing e Inconsistencies in the receiving substrate For example some membranes particularly if treated with detergents can contain hydrophobic patches that lead to inconsistent absorption characteristics Particulates or dust on the surface of nitrocellulose membranes can also result in inconsistent absorption e Static electricity If static is present either caused by dry ambient conditions low relative humidity or by the receiving substrate e g un backed nitrocellulose can generate a significant static charge inconsistencies in dispense may be expected as drops are deflected from their trajectory If possible use anti static bars and work in a slightly humid environment to overcome this problem BioDot Dispensers B 1 T Operating Manual Version 1 5 Page 47 CHAPTER FOUR CLEANING AND MAINTENANCE OF DISPENSERS Background This chap
59. ifier e Cover catch the dispensed stream of fluid from the dispenser so that fluid is no longer dispensed onto the web material and press the SET button again Note Refer to the Keyence Corporation product information for complete calibration instructions BioDot Dispensers B I Operating Manual Version 1 5 Page 68 Loosen Setscrew On The Rear Of The Holder Adjust Sensor And Retighten Adjust Sensor Up Or Down Figure 12 Contact Information Support Service If you encounter difficulty with the installation or of your unit or if you are missing any component from your order please contact Support Service at 949 440 3685 or E mail support biodot com Please do not attempt to disassemble factory assembled instruments or components without first speaking with a Support Service representative Doing so may void your warranty Note A decontamination form must be filled out prior to the return of any dispenser or system Please contact the e mail address above for the correct form BioDot Dispensers B D O T Operating Manual Version 1 5 INDEX A AirJet Quanti Dispenser 6 AirJet Quanti 2300 Specifications 6 AirJet Quanti 2400 Specifications 7 AirJet Quanti 3000 Specifications 6 Controlling Dispense Rate Volume 40 Illustrated 8 Line and Dot Dispensing 41 Line Width 40 Operation 39 Priming 41 AMP connector 12 B Back side defined 2 BioJet Q
60. ing of cables factory assembled to assure proper connection between the syringe pumps and between the syringe pumps and the system platform The cable assembly connector is the connection head that attaches to the syringe pump 15 pin connector Tools Equipment Set of Allen wrenches provided with the platform system Procedures BioJet Quanti 3000 Installation Unpacking 1 Remove the BioJet s support arm and reagent supply lines from the packing container carefully remove from inner wrapping material 2 Locate the packing checklist that is provided with the instrument Please go over this list to confirm that all ordered parts tools and accessories are accounted for If an item is missing please see Support Service in Appendix D of this manual 9 Note BioJet dispensers are fully assembled on arrival and attached to a small mounting bracket by which it is mounted to the support arm Please refer to the Dispenser Specifications section if you would like information on basic construction of the BioJet Quanti Please DO NOT attempt to take a dispenser apart without first contacting BioDot Support Service Attempting to disassemble a dispenser could damage the instrument and will void your product warranty BIODOT BioDot Dispensers Operating Manual Version 1 5 Installation The following installation instructions apply to both the 3000 model BioJet 1 Ensure platform power is OFF 2 Installthe Syringe Pump se
61. inge pump where the plunger is Editing Existing Programs To edit a program you simply modify the web handling parameters for that program under the EDIT sub menu 1 Select EDIT under the PROGRAM Reel to Reel platform or PATTERNS XYZ or ZX platform menu Select the program to be edited using the arrow keys on the terminal keypad and press the ENTER key 2 Make necessary changes using the arrow keys to select the menu options to be edited and entering new data on the terminal keypad Press the ENTER key after each new entry 3 SAVE the new data Using the ESC key return to the main menu and then select SAVE DATA under the DATA STORAGE menu The Edit Sub Menu The EDIT sub menu contains all the information to dispense lines or dots from one or more of the BioJet Quanti or AirJet Quanti dispensers The Syringe pump and Frontline dispensers are configured under the CONFIGURATION menu see Creating and BioDot Dispensers B I Operating Manual Version 1 5 Page 31 Deleting Programs A complete description of the EDIT sub menu is given in the table below and in the following section EDIT Sub Menu Options Defined Note The Frontline and Syringe Pump dispensers configured under the CONFIGURATION menu BioDot Dispensers B T Operating Manual Version 1 5 Page 32 Reel to Reel Platform Dispensing Menus DOT Task Not Available XYZ and ZX Platforms Dispensing Menus EDIT S
62. ispensers B I O D O T Operating Manual Version 1 5 APPENDIX B BIOJET DISPENSER RATE TABLES Page 61 BioJet Quanti Line Dispensing Parameters 50 100 uL Syringe 0 2 to 5 0 uL cm Dispense Rate 50 uL Syringe 100 uL Syringe RATE DROP ON RATE DROP ON RATE DROP ON RATE DROP DROP ON VOLUME PITCH TIME VOLUME PITCH TIME VOLUME PITCH TIME VOLUME PITCH TIME ul cm nl mm msec ul cm nl mm msec ul cm nl mm msec ul cm nl mm msec 0 2 4 17 0 21 0 20 1 0 20 83 0 21 0 20 2 4 17 21 0 20 1 2 25 00 21 0 20 6 25 0 31 0 20 33 33 0 33 0 25 8 33 42 0 20 37 50 31 0 30 8 33 0 42 0 20 50 00 0 50 0 50 12 5 63 0 20 50 00 42 0 50 25 4 17 0 17 0 20 1 1 25 00 0 23 0 20 3 4 17 14 0 20 1 3 33 33 26 0 20 6 25 0 25 0 20 33 33 0 30 0 25 8 33 28 0 20 50 38 0 50 8 33 0 33 0 20 50 00 0 45 0 50 12 5 42 0 20 54 17 42 0 55 10 42 0 42 0 20 1 2 25 00 0 21 0 20 4 8 33 21 0 20 1 4 29 17 21 0 20 0 3 4 17 0 14 0 20 33 33 0 31 0 30 12 5 31 0 20 50 36 0 50 6 25 0 21 0 20 50 00 0 42 0 50 16 7 42 0 20 66 67 48 0 67 8 33 0 28 0 20 1 3 27 08 0 21 0 20 5 12 5 25 0 20 1 5 33 33 22 0 25 12 50 0 42 0 20 33 33 0 26 0 25 16 67 33 0 20 50 33 0 50 4 6 25 0 16 0 20 54 17 0 42 0 30 25 5 0 20 75 5 0 70 8 33 0 21 0 20 1 4 29 17 0 21 0 20 6 16 66 21 0 20 1 6 33 33 21 0 25 12 50 0 31 0 20 43 75 0 31 0
63. ividual pattern PRIME DISPENSER SYSTEM UTILITIES platforms system statistics and the operation of system components tracking line inspection marking etc CONFIGURATION DATA STORAGE SYSTEM INFO All platforms deals with hand held terminal software information Please take a moment to look through the following menu hierarchy trees which begin on the following pages The first example will be the BioDot Reel to Reel platform The second menu tree will be a tabletop platform BioDot Dispensers B 0 D T Operating Manual Version 1 5 BIODOT REEL TO REEL V1 211 FACTORY MODE PRESS ENTER NOTE VALUES OF 65535 MAIN MENU PROGRAMS PRIME DISPENSER SYSTEM UTILS EDITe DELETE DELETE PROGRAM 1 PRESS GO TO CONFIRM OR ABORT TO CANCEL FILE NAME EDIT PROGRAM NAME 1 VALUE FEED CONTINOUS LENGTH 4 0 VALUE SPEED mm s 5 0 VALUE mm s2 100 VALUE DRIVE CAPSTAN YES NO DIP TANK NO YES DIS TRACK YES TAKEUP TRACK YES NO INSPECTION NO YES TCM s YES NO TANDEM BJQ 1 TANDEM BJQ 2 TANDEM AJQ 1 p TANDEM AJQ 1 ACTIVE YES NO RATE uL cm 5 00 VALVE TRANS 1 ms 75 VALVE TRANS 2 ms 75 VALVE p TANDEM BJQ 2 ACTIVE NO YES RATE uL cm 1 00 VALVE VOL nL 31 0 VALUE PITCH mm 0
64. le for the absence of the line quality you desire Most often it is a combination of issues Typical problems with line dispenses are as follows Breaks in lines Ensure that the proper dispense head height is set using the micrometer Optimum dispense height for BioJets is approximately 2 4 mm from web surface Check the system for air If bubbles are forming perform the procedure as described above for ridding the system of air bubbles If air is not visible in the system there can still be an air bubble trapped in the valve Perform the back flushing procedure outlined in the cleaning protocol The valve may also be clogged with particulates All fluids should be 0 2 um filtered if possible before dispensing to prevent the buildup of particulates in the valve poppet Some buildup of particulates in the valve is always expected For this reason it is essential that the cleaning procedures outlined in this manual be scrupulously followed Deflection of droplets Fluid is spraying away from the line causing satellites or splashing on the material This can be caused by a number of things The valve may be clogged with particulates Perform the cleaning procedure in Chapter Four There may be an imperfection in the tip particularly if the tip is a molded plastic tip Change the tip if possible and if possible change to a PBT tip if a molded plastic tip is being used Static buildup on the material can cause drops to be deflected from their tar
65. lowing more time for the reagent to be absorbed by the membrane can minimize this problem The drop pitch is one of four BioJet dispensing parameters that are interdependent changes in one of these four parameters must be accompanied by corresponding changes in the others These parameters are the dispensing RATE ul cm the DROP VOLUME in nl the DROP PITCH in mm and the ON TIME in msec These parameters form the basis for any BioJet dispensing program and their values should be selected based on the BioJet Dispenser Rate Tables that are included in Appendix B of this manual There are a variety of other dispensing program options nearly all of which are located under the EDIT menu on the hand held terminal and are described under Menu Tree Hierarchies and EDIT Sub Menu Options Defined later in this chapter Sample programs for the BioJet are included in the last section of this chapter see Test Programs for BioJet Dispensers The test programs serve a dual purpose First they are diagnostic programs and will determine proper functionality of the BioJets Second because it is advisable to perform these tests after BioJet installation they provide programming examples and practice to the new user Line Dispensing A line dispensing program is entered using the EDIT sub menu under the PROGRAM Reel to Reel or PATTERN XYZ or ZX menu You will need to create a program NAME select the DISPENSER or DEVICE to be used and select th
66. m MOTOR STEPS 7200 VALUE uSTEPS 64 VALUE TRAVEL 500 000000 VALUE TOP SPEED 117 VALUE NEG LIM 0 00 VALUE POS LIM 9999999 0 VALUE ENCODER REF ONLY NONE EDIT TANDEM BJQ Page 25 EDIT AIRJET EDIT AXES gt AXES 1 VALUE AXIS 1 e EDIT CAVRO r gt AXIS1 NAME CAPSTAN CPU BOARD 104 BUS UNITS mm MOTOR STEPS 200 VALUE uSTEPS 64 VALUE TRAVEL 61 500000 VALUE TOP SPEED 300 VALUE NEG LIM 0 00 VALUE POS LIM 9999999 0 VALUE ENCODER NONE REF ONLY TANDEM BJQ 1 TANDEM 2e TANDEM BJQ 2 CHANNEL INFO SYRINGE uL 250 VALUE FILL VOL uL 250 VALUE TYPE XP3000 XL3002 XL3004 XL3005 XL3006 XL3008 XL3000 STD XL3000 HI MODE HIGH RES LOW RES FORCE HALF QUARTER FULL ADDRESS 2 VALUE COM BIOS COM 1 COM 2 H CHANNEL 1 X OFF mm 0 00 VALUE Y OFF mm 0 00 VALUE TPU CHANNEL 1 VALUE p TANDEM BJQ 1 CHANNEL INFO SYRINGE uL 250 VALUE FILL VOL uL 250 VALUE TYPE XP3000 XL3002 XL3004 XL3005 XL3006 XL3008 XL3000 STD XL3000 HI MODE HIGH RES LOW RES FORCE HALF QUARTER FULL ADDRESS 0 VALUE COM BIOS COM 1 COM 2 s CHANNEL 1 X OFF mm 0 00 VALUE Y OFF mm 0 00 VALUE CHANNEL
67. mL Power Requirements Power Derived From a BioDot Dispensing Platform Power Derived From a BioDot Dispensing Platform Power Derived From a BioDot Dispensing Platform 1 Width is highly dependent on absorption properties of the substrate and fluid rheology 2 Dependent on fluid rheology Based on consistent and reproducible dispense of 0 05 BioTerge in water over a 30 cm path length 3 Based on 5uL cm over 30 cm path 4 Based on 250uL syringe and minimum usable tubing lengths BIODOT BioDot Dispensers Operating Manual Version 1 5 BioDot Dispensers Micrometer Shim Housing ZR04309 Needlehead ZR04308 ve 4 Setscrew 0 80 x 1 8 long Needle ZR04306 Spring C0180 024 0880S Gland Screw m ZR04307 056 ID x 060 W Spindle ZR04304 2 O Ring 101 ID x 070W Jamb Nut ror M Body 2904305 Fluid Inlet Ref Fitting KQH07 32 O Ring 239 ID x 025 W s 080 3 O Ring 055 ID x 016 W Nozzle Cap 1 140 3 Needle 1 110 2 AirJet BIODOT Page 51 Operating Manual Version 1 5 Page 52 Troubleshooting Platform Symptom Possible Cause 2500 3000 3050 3200 5000 AirJet General Tips Ensure that the proper dispenser has been selected the Program e Ensure
68. ning after programming Dispenser Errors When the platform detects an error condition it will pause to display the errors to the operator for corrective action The operator must acknowledge the error by pressing the ENTER key If the error occurred during a dispense cycle the cycle will be aborted and the slides retracted to the park position Inputting Programs Menu Hierarchies In this section we have provided a representation of the menu hierarchies within the hand held terminal software menus that pertain specifically to the dispensers For the sake of economy we have omitted all fields that do not pertain to the dispensing cycle We will refer to this hierarchy throughout the chapter please refer to your system platform manual for information on platform specific fields For a complete description of the hand held terminal and menu hierarchies please refer to the user manual There are six levels total in the hierarchy the main menu and five sub menus The top level represents the Hand held terminal main menu Hand held Terminal Main Menu Note There are slight menu variations between platforms The following menu trees are in FACTORY MODE Some of the selections will not be available to the end user when in non factory mode PROGRAM BioDot Dispensers B T Operating Manual Version 1 5 Page 24 REPEATS XYZ platform only used for programming dot matrices or rows of lines which require repetition of an ind
69. ogram an array of drops using the REPEAT function of small to medium size and dispense 0 05 BioTerge onto the dispensing nest for ease of assessment of dot quality The Dot Dispense Test program is outlined below BioDot Dispensers B T Operating Manual Version 1 5 Page 44 NAME Dot Dispense Test ACTIVE Yes DISPENSER BioJet Quanti 1 SHAPE Dot Z ENABLED Yes X START mm 10 Y START mm 10 DROP nl 100 TOTAL nl 100 DROPS 1 FREQ Hz 100 ON TIME ms 0 8 REPEAT 1 4 X OFFSET mm 5 Y OFFSET mm 0 REPEAT 2 4 X OFFSET mm 0 Y OFFSET mm 5 In Dispenser Sub Menu Dot Dispense Test Instructions Prime the dispenser prior to performing the test Examine the arrays of dots for drop consistency for satellite drops or spray or for missed dispenses The valve passes if dots are consistent none have been missed and there is no spray or satellite drop formation Line Dispense Test This program is designed to determine if the BioJets are capable of dispensing a consistent line of fluid under optimal dispensing conditions To do this program an array of lines of medium size and dispense fluid onto a suitable medium for ease of assessment of line quality Bare in mind that the results of this test can be affected by the material you are dispensing on Parafilm is a good material to use for this purpose as it often creates few
70. onserved between dispensed drops Rheology Refers to the flow properties of a dispensed fluid reagent Fluid Path Volume The volume of reagent within the dispensing system between the syringe pump and the dispenser tip release point Swept Volume The average volume of reagent that is retained by lost to the dispensing system Volume Recovery The percentage of total reagent that is actually dispensed total reagent in the dispensing system minus swept volume BioJet Quanti Dispensers This series of quantitative dispensers couples BioDot s drop on demand valve with one or two high resolution syringe pumps to meter precise amounts of reagent The following syringe pump sizes are available with each of these dispensers 50 ul 100 ul 250 ul 500 ul and 1 0 ml BioDot Dispensers B O T Operating Manual Version 1 5 Page 4 BioJet Quanti 3000 Specifications Power Air Requirements Power is derived from a BioDot dispensing platform 110 220 VAC 50 60 Hz this dispenser does not utilize air Dispensing Parameters Smallest Drop Volume 4 2 nl Drop Size Range 4 2 nl 1 0 ul Volume Range 0 5 ul 30 ul second dependent on fluid rheology Smallest Continuous Line Width 0 020 inches 0 5 mm dependent on fluid rheology and substrate properties Repeatability 1 Line dispensing and 3 Dot to Dot dispensing dependent on fluid rheology and drop size Fluid Path Volume 350 ul includes feed lines wit
71. orarily set AirJet body aside e Assemble two o rings item 14 onto spindle item 4 by sliding over steps in spindle Note It may be desirable to use an assembly aid such as some alcohol or water in order to more easily stretch the o ring over the diameter step in the spindle e Install o ring item 13 into the bore of the spindle so that it is fully seated It may be difficult to seat the o ring perfectly flat against the stop Some o ring distortion is permissible as long as light can pass through the o ring inside diameter e Assemble gland screw item 7 into the spindle and tighten Stop At this time verify that a test needle similar to item 6 can be installed into the rear of the assembly without snagging or damaging the previously assembled o ring The needle should slide smoothly and have a moderate amount of drag If damage has occurred replace o ring as in step e Be cautious when handling the needle as the tip may easily become damaged e Assemble o ring item 16 over the threads of the nozzle item 3 making sure it is seated against the nozzle flange e Assemble the nozzle into the small end of the spindle and tighten A Warning Do not over tighten as the threads of the nozzle are very weak and will break off easily e Thread the spindle into the body until the tip of the nozzle extends slightly beyond the front face of the nozzle cap Line up the second hole in the spindle with the hole in the feed line port Note Th
72. orm or PATTERNS XYZ or ZX platform menu Select the program name using arrow keys and press the ENTER key The program in now deleted PROGRAM and CONFIGURATION the Dispenser Programming Menus CONFIGURATION Menu The information in this menu controls the platform speed Z axis Z slide movement BioJet dispenser PRIME speed and Syringe Pump speeds Refer to menu trees on page 26 amp 27 EDIT DISPENSER EDIT DEVICE NAME Or EDIT TANDEM for the Reel to Reel this field selects the dispenser to BioJet or AirJet be configured 7 Note A device name will not appear in the field if dispensing with a Frontline dispenser or syringe pump alone where no BioJet AirJet device is installed AirJet Configuration Sub menus under EDIT CHANNEL INFO See4 X and4 Y below CHANNEL 1 X OFF mm A field used to inform the platform of the dispenser X axis location relative to the selected origin home in millimeters this is a factory setting Y OFF mm A field used to inform the platform of the dispenser Y axis location relative to the selected origin home in millimeters user should select TPU CHANNEL Is the assigned BioJet channel example BiojetQuanti 1 Channel 1 TPU channel 0 would be BioJet channel 1 on the rear panel BioJet Configuration Sub menus under EDIT CHANNEL INFO Same as AirJet Channel Info menus above X and Y off SYRINGE ul This is the syringe size in ul the value mu
73. pendent on fluid rheology BioDot Dispensers Operating Manual Version 1 5 Page 7 Line Width Range 0 04 inches 2 0 mm to 0 60 inches 15 0 mm line width is highly dependent on fluid rheology and substrate properties Repeatability 1 Line dispensing Swept Dead Volume approximately 1 8 ul Volume Recovery lt 98 AirJet Quanti 2400 Specifications The AirJet Quanti 2400 is the largest aerosol dispenser in this series with a nozzle needle size of 0 80 mm Power Air Requirements Power is derived from a BioDot dispensing platform 110 220 VAC 50 60 Hz Air source required 10 40 PSIG 0 70 2 80 Kg cm it must be a clean dry and filtered air source at constant pressure Dispensing Parameters Dispensing Range 1 ul cm 30 dependent on fluid rheology Line Width Range 0 08 inches 2 0 mm to 1 00 inches 25 0 mm line width is highly dependent on fluid rheology and substrate properties Repeatability 1 Line dispensing Swept Dead Volume approximately 1 8 ul Volume Recovery lt 98 The AirJet Quanti Dispenser Illustrated The AirJet Quanti dispenser with associated components is shown in Figure 2 These figures are representative of the 3000 2400 and 2300 models The AirJet is shown here in the context of a BioDot system platform with vertical and horizontal positioning stages Please take a moment to familiarize yourself with the basic layout of the dispenser before p
74. pensers are designed to install quickly and easily and few with tools required As we mentioned in the introduction to this manual the instructions given assume installation onto a BioDot system platform f you find that the information here is insufficient or not applicable to your particular dispensing application please contact BioDot Support Service see Appendix D F The goal of this section is to outline the steps in the installation of all models of BioDot dispensers Key Words System Platform Refers to the machine used for reagent application that incorporates a BioDot dispenser i e XYZ 3000 RR4500 Web Handling System etc Dispensing Platform The mounting surface for the attachment of dispensers the position of the dispensing platform is adjustable in the X and Y axis Supply Bottle The reservoir that holds the supply reagent Reagent Supply Line Teflon tubing used to carry reagent from the supply bottle to the syringe pump and or dispenser or directly to the web Support Arm A universal mounting bracket that a dispenser via a smaller mounting bracket is mounted to the support arm attaches in some platform systems to a vertical and or horizontal positioning stage BNC Also Bayonet or Coaxial connectors a BNC connector is used to interface BioJet dispensers with the system platform Vertical Z Axis Positioning Stage Also called the Z Slide An integrated dispensing component of certain BioDot dispensing platform
75. platform menu is the primary menu for dispenser programming It allows the operator to create select delete and edit programs The PROGRAM menu including the EDIT submenu where most of the dispenser programming fields are located is discussed in depth in Editing Existing Programs later in the chapter REPEATS and PRIME DISPENSER the Accessory Dispensing Menus REPEATS The REPEATS menu is only available in the XYZ or ZX platforms the Reel to Reel platform does not support repeat patterns The REPEATS menu is used to repeat an entire active program It is important to understand that this menu is separate from the REPEAT 1 2 and Z fields under the EDIT submenus for designing a program REPEAT 1 2 and Z are used to repeat a task withina program The REPEATS menu is used to repeat an entire program This is useful when you want to offset and repeat one or more programs For example you may wish to make thick lines from several parallel thin lines or to dispense identical patterns onto several different parts of the web To use the REPEATS feature it is helpful to envision the dispense area on your web as an array of square grid elements divided into a number of rows and columns Rows lie along the X axis while columns lie along the Y axis The sub menus for the REPEATS menu are BioDot Dispensers B T Operating Manual Version 1 5 Page 30 ROWS A field used to define the number of rows to be used for the pattern program
76. putting Programs and The Edit Sub Menu for details Priming the Dispenser A dispenser should be primed in preparation for both dispensing and cleaning You will use the PRIME DISPENSER menu to perform the priming sequence The priming speed is selected under the CONFIGURATION menu See REPEATS and PRIME DISPENSER The Accessory Dispensing Menus and PROGRAM and CONFIGURATION The Dispenser Programming Menus for details on priming the AirJet These sections are found in the Inputting Programs section of this chapter Configuring the Syringe Pumps See Syringe Pump Operation below Frontline Quanti Operation The Frontline dispenser itself requires no special programming as the dispense rate from a Frontline dispenser is simply that of the syringe pump To operate the Frontline dispenser you have only to properly install the specially designed dragging tip s set the angle and height of the dispenser per your application needs and configure the Syringe Pump to deliver the proper rate of flow see Syringe Pump Operation Syringe Pump Operation The Syringe Pump dispenser contains a high resolution stepper motor that is has 24 000 distinct steps or dispensing units per full stroke Syringe Pump dispensers can be used alone or in combination with another dispenser BioJet AirJet or Frontline to dispense reagent Syringe Pumps can be wired together to work in tandem in this case the two pumps signal each other at a given time the t
77. r thumbscrew on the base of the syringe plunger arm Slide the syringe plunger out of the syringe barrel by pushing gently downwards on the plunger arm and position the bottom end of the plunger in the plunger arm receptacle e Re tighten the plunger arm thumbscrew to secure syringe 7 Attach supply line tubing to the syringe pump e Feed supply line tubing into the supply reagent bottle via the HPLC connector provided and secure the lid in place attach the other end of the supply line to the left hand port of the 3 port valve on the syringe pump via the HPLC connector provided Screw the BioJet Plus dispense line into the output dispense side of the syringe pump 3 port valve assembly Right hand port e With Quanti dispensers Attach the other end of the dispenser line to the dispenser as discussed in the Dispenser Installation section e f dispensing with Syringe Pump only Attach an appropriate tip to the end of the supply line for direct dispensing per your application 8 Restore power and test Syringe Pump see syringe pump operation 9 Note We have not included specific testing protocols for the Syringe Pump as it is compared to the BioJet a far less complex dispenser to set up and use If problems arise please refer to the product literature from Cavro Scientific Instruments Inc or contact BioDot Support Service BIODOT BioDot Dispensers Operating Manual Version 1 5 Page 21 CHAPTER THREE DISPENSER OPERATI
78. ransition time before the end of their stroke so that the other can begin its stroke at the appropriate time thereby maintaining a constant pressure in the supply line tubing BioDot Dispensers B T Operating Manual Version 1 5 Page 42 The programming fields for the operation of the syringe pump with or without another dispenser are located in the CONFIGURATION menu Where a Syringe Pump is used alone or in combination with a Frontline Quanti Dispenser only those fields related to Syringe Pump configuration will be shown Test Programs for BioJet Dispensers This section is designed to help the user understand how to determine whether the BioJet Quanti dispenser is functioning correctly and to how diagnose any observed problems When using BioJets for any dispense it must be remembered that the quality of the dispense observed can be affected by factors other than the performance of the valve itself including characteristics of the fluid being dispensed the material being dispensed onto and ambient environmental conditions such as humidity As a result determination of the performance of the BioJet valves themselves must be performed under optimized conditions Ideally the fluid used should be water containing a small amount of detergent e g 0 05 BioTerge All fluids used should ideally be degassed and 0 2um filtered before use The protocol outlined here allows the user to focus on the operation of the valve itself
79. roceeding to Chapter Two Dispenser Installation BioDot Dispensers B T Operating Manual Version 1 5 Page 8 Needle Micrometer Barrel AirJet Adjustable Mounting Bracket E lt 7 Air Inlet Crown Tip Figure 2 Frontline Quanti 1000 Dispenser This dispenser couples a micro tube dispenser with one or two syringe pumps based on dispensing rate to meter precise amounts of reagent or ink The following syringe sizes are available 50ul 1001 250yl 500 and 1 0 Power Air Requirements Power is derived from a BioDot dispensing platform 110 220 VAC 50 60 Hz this dispenser does not utilize air BIODOT BioDot Dispensers Operating Manual Version 1 5 Page 9 Dispensing Parameters Minimum Dispense Volume 0 1 ul second dependent on fluid rheology Minimum Continuous Line Width 0 3 mm line width is highly dependent on fluid rheology and substrate properties Maximum Fluid Path Volume lt 350 ul using a 250 ul syringe and 20 cm of standard tubing Repeatability 1 dependent on fluid rheology Volume Recovery 95 The Frontline Quanti Dispenser Illustrated The Frontline Quanti dispenser is shown in Figure 3 Please take a moment to familiarize yourself with the basic layout of the dispenser before proceeding to Chapter Two Dispenser Installation Reagent Line B Fixture Locating Pin x 2 Spring Release Fixture Screw Mounting Hole Sl
80. rom the system Ensure that all reagent lines are connected securely and are free of clogs and leaks Check all connections Check lines and plunger seal integrity on the syringe Replace seals or syringe as necessary Ensure that the pitch and on time values are appropriate for the dispense volume entered Pressure build up due to incorrect on time may lead to leaks in the system Excessive air bubbles develop in the reagent lines De gas the fluid before attempting to dispense Check all connections for air tightness Check valve for leaks see protocol attached Slow the prime speed BioJet Valve does not prime correctly the fluid stream is deflected is not continuous or splashing is extreme Check the valve with a new tip If using a molded tip change to a PBT tip if possible If this does not solve the problem the valve may be clogged Perform cleaning protocol in Chapter Four and retry the valve If the problem persists replace the valve BioDot Dispensers Operating Manual Version 1 5 Page 58 During dispensing the BioJet Valve does not dispense a continuous line Line quality is derived from a number of factors including operation of the valve the characteristics of the material being dispensed onto the characteristics of the fluid being dispensed and environmental conditions Do not automatically assume that any one of these parameters is responsib
81. ront side Back side Refers to elements towards the back of the machine or away from operator Front side Refers to elements towards the front of the machine or Operator nearer the operator The first chapter of the manual Chapter One Dispenser Specifications begins on the following page BioDot Dispensers B I D Operating Manual Version 1 5 Page 3 CHAPTER ONE DISPENSER SPECIFICATIONS Background This chapter discusses the specifications including dispensing parameters and structural components of each of the BioDot dispensers F The goal of this section is to provide an overview of the capabilities and structure of each dispenser type this section will serve as a reference tool for the installation operation and maintenance of BioDot dispensers Key Words Drop on Demand Technology Dispensing technology behind the Quanti line of dispensers that is based on the combination of a solenoid valve to form drops and a syringe pump to meter reagent a Quanti dispenser is capable of precisely defined drop sizes or line volumes that are determined by the stepping resolution of the syringe pump and the size of the syringe Web The material onto which fluid is dispensed Line Volume The volume of reagent contained in a line per unit of length Line Width The width in inches of a dispensed line dependent on fluid rheology and properties of web material Repeatability The degree to which drop volume is c
82. roper dispenser has been selected in the Program Ensure that all reagent lines are connected securely and are free of clogs and leaks Ensure that the proper volume is entered in the Program Programmed volume should not be less than 0 2 ul cm or this drop size Drop size may vary depending on application Ensure that the pitch and on time values are appropriate for the dispense volume entered Consult dispenser tables Ensure that BioJet valves and PBT tips are clean and free of obvious defects Ensure that the BioJet cables are connected properly Ensure that the proper dispense head height is set using the micrometer Optimum dispense height for BioJets is approximately 2 4 mm from web surface Ensure that the dispenser has been properly primed Ensure that the appropriate syringe size has been configured Ensure that the transition times Trans 1 and 2 are set correctly for the fluid you are dispensing These settings are determined empirically and may differ for each fluid and tandem pump set Having these settings correct will ensure that no gaps or bulges in dispensed lines will occur upon pump transition BioJet Valve does not fire as determined by the absence of fluid coming from the valve and the absence of the distinctive sound of the valve firing Ensure that the proper dispenser has been selected in the program Ensure that the BioJet cables are connected properly Fluid appears to be leaking f
83. s facing the front side of the unit Do not skip any connectors 7 Connect the opposite end of the cable to the BioJet by pushing the cable connector onto the exposed pins on the back of the dispenser without regard to connector orientation 8 Connect the free end of the reagent supply line from the syringe pump to the BioJet inlet port This may be a bore end or a BioJet Plus end Refer to Figure 1 Insert the bore end over the barbed fitting and slide the green locking sleeve over the connection If it is a BioJet Plus fitting screw the fitting into the top of the BioJet valve and tighten 9 Restore power and test dispenser see BioJet Quanti 3000 Operation in Chapter Three 9 Note If your system includes a Line Presence Inspection System you will need to install the fiber optic wires for this system See Line Presence Inspection System in Appendix C for information on installation of this optional system BIODOT BioDot Dispensers Operating Manual Version 1 5 Page 15 AirJet Quanti 3000 2300 and 2400 Installation Unpacking 1 Remove the AirJet s and air and reagent supply lines from the packing container carefully remove all components from inner wrapping material 2 Locate the packing checklist that is provided with the instrument Please go over this list to confirm that all ordered parts tools and accessories are accounted for If an item is missing please see Support Service in Appendix D of this manual No
84. s solidified in the valve that can be re dissolved by the use of a solvent which is compatible with all materials in the BioJet then that solvent may be used for this procedure Procedure for AirJet assembly AirJet Specifications Topic AirJet Quanti 2300 AirJet Quanti 2400 AirJet Quanti 3000 Repeatability 1 Line Dispensing 3 1 Line Dispensing 3 1 Line Dispensing 3 Nozzle Needle Size 0 6mm 0 8mm 0 Dispensing Range Lower Limit 1 uL per cm 1 uL per cm 1 uL per cm Upper Limit 30 uL per cm 2 80 pL per cm 2 22 uL per cm 2 Line Width 1 0 04 to 0 60 0 08 to 1 00 0 02 to 0 24 BioDot Dispensers Operating Manual Version 1 5 Page 50 Typical Width Range 1 mm to 15 mm 2 mm to 25 mm 0 5 mm to 6 mm Swept Volume 4 1 8 uL approx 1 8 uL approx 1 5 uL approx 96 Recoverable 9896 9896 9896 Requirements Between 10 40 PSIG clean Between 10 40 PSIG clean Between 10 40 PSIG clean amp dry amp dry amp dry Air Pressure Tubing 1 8 ID x 1 4 OD Tubing 1 8 ID x 1 4 OD Tubing 1 8 ID x 1 4 OD Tygon Tygon Tygon Feed Line Tubing 1 16 ID TFE Tubing 1 16 ID Tubing 1 32 ID TFE Materials Stainless Steel Stainless Steel Titanium Stainless Steel Available Syringes 100uL 250uL 500uL 1mL 50uL 100uL 250uL 500uL 1mL 50uL 100uL 250uL 500uL 1
85. s that is height adjustable Z axis through the use of a micrometer a dispenser is mounted to the vertical positioning stage via a support arm Horizontal Y Axis Positioning Stage An integrated dispensing component of certain BioDot dispensing platforms that is height adjustable in the y axis through the use of a micrometer a dispenser and associated components are mounted to the horizontal positioning stage via a universal mounting bracket Line Presence Inspection System An optional dispensing system component consisting of fiber optic sensors that detect the presence absence of a dispensed line absence of a line is signaled and triggers the placement of an ink line on the web material see Line Presence Inspection System in Appendix B for more details Inlet Port A point on the dispenser where a reagent feed line attaches This port may be attached to the reagent line via a plastic fitting and ferrule barbed fitting and sleeve collar or screw in plastic fitting Quick Connect Fitting This fitting is used to make the airtight airline connection on a BioDot AirJet HPLC Fitting The ferrule and nut style fitting used to make the watertight reagent supply line connection on the AirJet BioDot Dispensers B I T Operating Manual Version 1 5 Page 12 AMP An AMP brand name connector is a larger round usually black panel pin and socket connector interfaces with a jack on the back of the unit Cable Assembly The group
86. s the information specific to their system You should read through the sections relevant to your dispenser at least once from beginning to end in order to learn how to properly install operate and maintain your dispenser s a safe and efficient manner Please refer to the Table of Contents preceding this introduction to locate sections specific to your system Chapter Layout Each chapter of this manual with the exception of Chapter One Dispenser Specifications which contains no Procedure section is organized into two sections which are outlined below Background The Background section includes a brief introduction to the chapter followed by a listing of Key Words key terminology defined and any Tools and Equipment you will need for the procedure that follows Key Words are italicized the first time they are used in a chapter to indicate that a definition is listed in the Background section Procedures The Procedures section contains step by step instructions for the task being performed Installation Physical Setup etc and comprises the bulk of each chapter Goals Notes and Cautions Throughout the manual you ll find margin icons or emphasized sections of text designed to draw your attention to key information The three types of emphasized text found in this manual are Goals Notes and Warning Goals A goal for the information presented is included in the Background section of each chapter and is emphasized with a
87. sets the pattern state to active or inactive this over rides the SELECT menu option DISPENSER not in simultaneous dispense software There are n of these menu items one for each dispenser for which the unit is configured where the number nis the dispenser number from left to right when facing the platform The operator selects a program to edit by using the arrow keys to scroll through the options and select the desired dispenser and the ENTER key to activate A checkmark subsequently appears after the dispenser selected current SHAPE This is a toggle field that controls the type of dispense pattern either a dot or a line Selecting DOT will enable the pattern to dispense dots Selecting LINE will enable the pattern to dispense lines however dots can be dispensed in the LINE task setting see BioJet Quanti 3000 Installation for details Some of the following menu items will change depending on whether dot or line is chosen Z ENABLED This is a toggle action that controls the Z axis When this function is ON the Z axis will move up and down during the pattern and the Z UP and Z DOWN fields will be displayed see below If this function is OFF then the Z axis will stay in the up most position This field is displayed for both LINE and DOT tasks DISPLAYED FOR LINE SHAPE ONLY LENGTH mm This field allows you to program the length in mm of your dispensed line in either the X or Y direction The stan
88. st match the actual syringe on the pump so the programmed dispense volume can be correctly computed FILL VOLUME ul This is the volume the syringe will draw it should be equal to or less than the syringe volume FORCE QUARTER HALF FULL FORCE is the force achieved at the end of travel during the syringe plunger motion it BioDot Dispensers B 1 T Operating Manual Version 1 5 Page 29 must be controlled to prevent damage to the syringe FORCE should be selected as follows QUARTER If using syringes 100 ul or smaller HALF If using syringes 250 ul or 500 ul FULL If using syringes 1 0 ml or larger PRIME ul sec This sets the syringe velocity when priming the BioJet Quanti BioJet Quanti 3000 Acceptable numbers range from 2 to 1000 with a typical value of 30 BioJet Quanti 3000 for a 250 ul syringe FILL ul sec This sets the syringe velocity when filling the syringe from the reagent reservoir Acceptable numbers range from 5 to 4000 with a typical value of 2000 for all syringe sizes WASTE X The X axis location where the dispenser will move to prime WASTE Y The Y axis location where the dispenser will move to prime Please remember that there are other CONFIGURATION menu options which are specifically used to configure the system platform platform HOME speed etc These are discussed in the appropriate system platform manual PROGRAM Menu The PROGRAM for the Reel to Reel platform or PATTERNS for the XYZ or ZX
89. te AirJet dispensers are fully assembled on arrival Please refer to Figure 2 in Chapter One Dispenser Specifications if you would like information on basic construction of AirJet Dispensers A Warning Please DO NOT attempt to take an AirJet apart without first contacting BioDot Support Service Attempting to disassemble a dispenser could damage the instrument and will void your product warranty Installation The following installation instructions apply to the 3000 2400 and 2300 models of AirJet Dispensers 1 Ensure platform power and air pressure are off 2 Install the Syringe Pump see Syringe Pump Installation Attach the support arm assembly AirJet mounting bracket attached to the dispensing platform using the four No 6 32 x 1 2 inch long socket head cap screws provided if necessary it is usually already mounted on the platform Secure the AirJet inside the mounting bracket Position the AirJet inside the split clamp with the air and reagent line inlet ports facing back Tighten the split clamp knob to secure the AirJet Attach the supplied air line to the air inlet port lowermost port in the lower back portion of the AirJet by screwing the 1 4 inch quick connect fitting to the AirJet air inlet port see Figure 7 8 Attach the other end of the airline to the mating receptacle labeled AIRJET or DISP n where n is the AirJet number from left to right where 1 or more AirJet dispensers are installed on the system pla
90. ter describes preventative maintenance and cleaning procedures for the BioJet Quanti 3000 AirJet Quanti 3000 2400 and 2300 Frontline Quanti 1000 and Syringe Pump dispensers To achieve optimum performance and maximum life from your dispenser it is recommended that you clean the dispenser after each period of use and perform a more stringent follow up cleaning regime on a weekly basis To minimize clogging of the dispensers and or dispensing tips and the attendant necessity of performing mechanical cleaning e g sonication back flushing which can damage dispenser valve components we strongly recommend the strict adherence to the cleaning protocols described in this chapter F The goal of this chapter is to provide the user with a Daily Cleaning protocol Weekly Cleaning and Decontamination protocols that are appropriate for your dispensing application and Unclogging methods for troubleshooting clogged dispensers or dispensing tips Key Words None Tools Equipment e Set of Allen wrenches provided with the unit e BioTerge detergent e Jetwash detergent e Deionized water Procedure Cleaning and Maintenance of BioJet AirJet and Frontline Dispensers Daily cleaning We recommend the following cleaning procedure for the routine maintenance of your dispensers This procedure should be performed after each period of use In general reagents should not be allowed to dry in the dispensers or their supply lines 1 Purge lines of rea
91. tform rear panel Insert the fitting into the air outlet receptacle and twist clockwise to lock in place see Figure 7 gm 27 Note There may be slight differences between system platforms the label assigned to the AirJet air outlet receptacle 9 Attach the reagent supply line via the 74 28 HPLC fitting provided to the supply line inlet port uppermost port in the lower back portion of the AirJet see Figure 7 BioDot Dispensers B O T Operating Manual Version 1 5 Page 16 10 Locate the AirJet air pressure gauge on the front of the system platform dispensing module Lower AirJet air pressure gauge to a minimal pressure before AirJet operation Turn the knob counter clockwise to lower the pressure to an appropriate operation level per your dispensing application Needle Micrometer Barrel 7 K MARKERM AIRJET From Rear Panel AirJet Fitting AirJet Regulator JET Adjustable Mounting Bracket The adjustment knobs of the front panel air regulators fully inserted at the maximum pressure setting prior to shipment to prevent damage to the knobs The gauge pressure should be lowered to a minimal pressure before air pressure is applied to the unit Reagent Line From Pump To AirJet Supply Connection Figure 7 Airline Quick Connect Fitting 11 Restore power and air pressure and test the AirJet see Chapter
92. that all reagent lines are connected securely and are free of clogs and leaks e Ensure that the proper volume is entered in the Program Programmed volume should not be less than 1 e Ensure that the dispenser has been properly primed e Ensure that the appropriate syringe size has been configured e Ensure that the airlines are connected properly and the appropriate amount of pressure is applied to the AirJet e Ensure the micrometer atop the AirJet is open sufficiently e Ensure that the dispense head is at an appropriate distance from the surface of the substrate approx 1 3 cm e Ensure that the air pressure is set appropriately The AirJet is commonly operated at 8 10psi e Ensure that the transition times Trans 1 and 2 are set correctly for the fluid you are dispensing These settings are determined empirically and may differ for each fluid and tandem pump set Having these settings correct will ensure that no gaps or bulges in dispensed lines will occur upon pump transition AirJet Breaks in lines e Check air pressure height of dispense head above substrate and opening of micrometer Generally air pressure is operated at 8 10 psi and the micrometer is set at 1 3 for commonly dispensed fluids such as gold conjugates or blocking reagents Alter one parameter at a time until line quality improves BioDot Dispensers B Operating Manual Version 1 5 Page 53 AirJet 3000 Ass
93. uanti Dispenser BioJet Quanti 3000 Specifications 4 Illustrated 5 Jacks 12 Line and Dot Dispensing 37 Line Parameters 1 0 ml syringe 57 Line Parameters 250 ul syringe 56 Line Parameters 50 ul syringe 55 Operation 36 Priming 37 Programming Considerations 36 Configuration Menu 28 BioDot Dispensers Appendix D Additional Resources Page 69 D Dispense Cycle Aborting 23 Error Messages 23 Starting 23 Dispensing Line Parameters 1 0 ml syringe 57 Line Parameters 100 ul syringe 55 Line Parameters 500ul syringe 56 Downstream defined 2 E Edit Sub Menu 30 F Frontline Quanti Dispenser Illustrated 9 Installation 17 Operation 41 Specifications 8 Front side defined 2 H Hand held Terminal Main Menu 24 Menu Hierarchies 23 Running a Dispense Cycle with 22 Operating Manual Version 1 5 BIODOT INDEX 70 I 5 Installation Procedures Syringe Pump Dispenser AirJet Quanti 15 Illustrated 10 BioJet Quanti 12 Installation 18 Frontline Quanti 17 Operation 41 Syringe Pump 18 Specifications 10 Syringe Installation 20 L T Line Presence Inspection System 59 Support Service BioDot Contact Information 62 P U Installation 12 Upstream defined 2 Prime Dispenser Menu 28 Program Menu 29 Programming Create a New Program 27 Creating Editing Programs 27 Delete an Old Program
94. ub Menu for BioJet and AirJet Dispenser EDIT NAME names of created programs Tandem BJQ 1 or AJQ 1 3 checkmark if enabled BioJet AirJet ACTIVE YES NO ACTIVE YES NO RATE ul cm RATE ul cm VOL nl TRANS n ms PITCH Hz ON TIME ms TRANS n ms EDIT Sub Menu for BioJet EDIT NAME names of created programs ACTIVE YES NO DISPENSER BioJet n Z ENABLED YES NO SHAPE LINE DOT LENGTH mm POLARIZATION SPEED mm sec ACC mm sec X START mm Y START mm Z UP mm Z DOWN mm REPEAT 1 These options UNDER BIOJETQUANTI 1 DROP nl PITCH mm VOLUME pL cm ON TIME msec REPEAT 2 not available REPEAT Z in ZX platform X START mm Y START mm Z UP mm Z DOWN mm REPEAT 1 These options UNDER BIOJETQUANTI 1 DROP nl TOTAL nl DROPS FREQ H2 ON TIME msec REPEAT 2 not available REPEAT Z in ZX platform BioDot Dispensers Operating Manual Version 1 5 Page 33 EDIT ACTIVE YES NO DISPENSER AirJet n Z ENABLED YES NO SHAPE LINE DOT LENGTH mm POLARIZATION SPEED mm sec ACC mm sec X START mm Y START mm Z UP mm Z DOWN mm EDIT Sub Menu for AirJet Dispenser NAME names of created programs X START mm Y START mm Z UP mm Z DOWN mm VOLUME uL REPEAT 1 These options REPEAT 2 not available REPE
95. ure support arm attach plate to vertical positioning stage using the supplied hardware Refer to Figure 8 Loosen the locking screw depress the spring lock and slide the dispenser to the desired location Release the spring lock and secure the position with the locking screw 5 Screw the supply line tubing assembly s from the syringe pump into inlet ports on the downstream side of the dispenser 6 Restore power and test dispenser see Chapter Three Dispenser Operation for dispensing instructions gt ous Note We have not included specific testing protocols for the Frontline dispenser as it is compared to the BioJet a far less complex I dispenser to set up and use If problems arise perform the appropriate Weekly Cleaning and Decontamination Procedure in Chapter 4 Cleaning and Maintenance of Dispensers and or contact BioDot Support Service BioDot Dispensers B T Operating Manual Version 1 5 Page 18 Attach Head Assembly Attach Reagent Line Loosen Locking Screw Depress Spring Lock To Adjust Position Figure 8 Syringe Pump Installation Unpacking 1 Remove the syringe pump s from the packing container and carefully remove from the plastic wrapping 2 Locate the packing checklist that is provided with the instrument Please go over this list to confirm that all ordered parts tools and accessories are accounted for If an item is missing please see Support Service in Appendix D of this m
96. y hand e Trial fit micrometer item 12 in place at end of micrometer housing and note position of fixed scale graduations relative to incoming fittings on AirJet body Add shims item 11 as necessary so that graduations are on opposite side of AirJet body from incoming air and fluid fittings Once established remove the micrometer Warning Make sure barrel of micrometer is rotated slightly open to allow body of micrometer to be fully seated If micrometer barrel is difficult to rotate loosen locking ring near base of micrometer e Rotate micrometer to close position and note that needle extends slightly from nozzle when viewed from end Loosen the setscrew located in the knurled portion of the micrometer Rotate and slide the micrometer barrel so that zero hash mark and edge of micrometer barrel are positioned at the zero hash mark on the fixed micrometer scale Tighten setscrew and rotate micrometer barrel to confirm proper scale match up at seated position If the position cannot be reached add a few more shims then try to readjust e Functionally test and observe that liquid flow begins at approximately 1 to 2 mils from fully closed L B BioDot Dispensers B Operating Manual Version 1 5 Page 56 APPENDIX A TROUBLESHOOTING BioDot Dispensers B i Operating Manual Version 1 5 Page 57 BioJet Dispenser Troubleshooting Guide PROBLEM POSSIBLE SOLUTIONS General Tips Ensure that the p

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