G1328-90013 - Agilent Technologies
Contents
1. Notices Agilent Technologies Inc 2006 2011 2012 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61328 90013 Edition 10 2012 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement w2. Exchanging the When cable damaged orwhenno 10 minutes See Position Sensing Switch on position sensing start signal is sent when page 32 switch switching to the inject position 26 1260 Infinity Manual Injector Maintenance 4 Flushing the Manual Injector CAUTION Damage through crystal formation The use of aqueous buffers or salt solutions can lead to crystal formation which may cause scratches on the injection seal gt Always rinse the valve with water after using aqueous buffers or salt solutions 1 Switch the valve to the INJECT position 2 Use the pump to flush the sample loop and seal grooves 3 Use the needle port cleaner supplied with the valve and syringe to flush the needle port and vent capillary Needle port Syringe Needle port cleaner Figure 11 Needle port Cleaner 1260 Infinity Manual Injector 27 4 Maintenance Cleaning the Manual Injector The manual injector base should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent 28 1260 Infinity Manual Injector Maintenance Injection Valve Seal When e Poor injection volume reproducibility Leaking injection valve Tools required Description Hexagonal key 9 64 inch Parts required p n Description 1 5068 0052 Rotor seal PEEK 4 1 Loosen the three stator screws 2 Remove the stator head 1260 Infinity Manual Injector
3. Sample loops stainless steel Description Sample loop 5 pL Sample loop 10 pL Sample loop 20 pL Sample loop 50 pL Sample loop 100 pL Sample loop 200 pL Sample loop 500 pL Sample loop 1 mL Sample loop 2 mL Sample loop 5 mL Sample loops PEEK Description Sample loop 5 pL Sample loop 10 pL Sample loop 20 pL Sample loop 50 pL Sample loop 100 pL Sample loop 200 pL Sample loop 500 pL Sample loop 1 mL Sample loop 2 mL Sample loop 5 mL 1260 Infinity Manual Injector 1260 Infinity Manual Injector Appendix J Agilent Technologies on Internet 42 Additional information ea Agilent Technologies 41 6 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the internet at http www chem agilent com 42 1260 Infinity Manual Injector Index A Agilent on internet 42 APG remote 13 B buffers 27 C capillary connections 14 cleaning the manual injector 28 D damaged packaging 8 delivery checklist 8 description 6 E exchanging injection seal 29 position sensing switch 32 F flow cell solvent information 19 flow connections 14 flushing the manual injector 27 information oninternet 42 injecting sample 6 INJECT 6 22 1260 Infinity Manual Injector injection seal 29 tefzel 20 vespel 20 installation 10 installing the manual injector 10 internet 42 42 L leak channel 16 leak drainage 16 leaks 14 LOAD 6 2
4. of the two vent capillaries ports 5 and 6 and the needle port must be at the same level to prevent siphoning see Figure 7 on page 15 1 Connect the pump outlet capillary to port 2 2 Connect the column compartment inlet capillary to port 3 3 Connect the sample loop between ports 1 and 4 14 1260 Infinity Manual Injector Installing the Manual Injector 2 low Connections 4 Connect one vent capillary supplied with valve to port 5 and one to port 6 fA From pump outlet Sample loop Waste Vent tube To column ae Figure 6 Flow Connections a Vent capillaries ports 5 and 6 lt anaa Vent capillaries and needle port at the same level Figure 7 Vent Capillaries 1260 Infinity Manual Injector 15 2 Installing the Manual Injector Leak Drainage WARNING Leaking injector fittings In the event of a leak solvent will drop into the leak channel in the baseplate from where it is channelled to the front and back of the baseplate gt Check the manual injector fittings periodically for signs of leakage Leak channel Figure 8 Leak Drainage 16 1260 Infinity Manual Injector 1260 Infinity Manual Injector 3 Using the Manual Injector Warning
5. 2 M make before break 6 needle type 21 needle port cleaner 27 needles 21 P position sensing switch 32 position sensor cable 13 precision 22 repair procedures injection seal 29 position sensing switch 32 S salt solutions 27 Index sample loops 6 sample volume 22 solvents acids 19 buffers 27 ethers 19 rinsing 27 salt solutions 27 steel corrosive 19 start cable 13 U unpacking damaged packaging 8 delivery checklist 8 Ww worldwide web 42 43 www agilent com In This Book This manual contains user information about the Agilent 1260 Infinity Manual Injector The manual describes the following introduction to the manual injector e installing the manual injector e using the manual injector maintenance of the manual injector e parts and materials and additional information Agilent Technologies 2006 2011 2012 Printed in Germany 10 2012 G1328 90013 fe Agilent Technologies
6. 260 Infinity Manual Injector 37 5 Parts and Materials for Maintenance Injection Valve Assembly Item wo 38 Manual Front Loading Injector Valve 600 bar 5067 4191 with starts cable complete assembly including operating instructions needle port cleaner vent tubes x2 and fittings 5 64 and 9 64 inch hex keys Includes items 1 8 p n 1535 4045 1535 4046 5068 0052 5068 0118 0100 1859 0100 1860 5068 0020 8710 0060 0490 1849 1400 3166 Description Bearing Ring Qty 1 replacement Isolation seal Rotor seal PEEK Stator ring Stator face Stator head Stator Screws 10 pack Hex key wrench 9 64 inch Position sensing switch Ring stand mounting bracket 1260 Infinity Manual Injector Parts and Materials for Maintenance 5 Injection Valve Assembly Figure 13 _Injection Valve Assembly NOTE Accuracy of sample loops The actual volume of a sample loop can differ by 10 for a 20ul loop Smaller loops can have a greater deviation and bigger loops a smaller one Use partial loop filling if you must know the actual injected volume 1260 Infinity Manual Injector 39 5 p n 0101 Parts and Materials for Maintenance 1248 0100 1923 0100 1922 0100 1924 0100 1921 0101 0101 0101 0101 0101 p n 0101 0101 0101 0101 0101 0101 0101 0101 0101 0101 40 1247 1246 1245 1244 1243 1241 1240 1239 1238 1242 1227 1236 1235 1234 1230
7. 29 4 Maintenance 3 Remove the stator ring 5 Install the new seal Ensure the seal is positioned as shown Rotor pin Needle hole Stator ring 4 Remove the seal 6 Install the stator ring Ensure the pin in the stator ring is aligned with the hole in the valve body and the position sensoring switch is back in place 30 1260 Infinity Manual Injector Maintenance 4 Injection Valve Seal 7 Insert the stator face onto the stator head 8 Install the stator head onto the valve Ensure the pin in the stator ring is aligned with the hole in the stator head 9 Secure the stator head in place with the stator screws Tighten each screw alternately 4 turn until the stator head is secure 1260 Infinity Manual Injector 31 4 Maintenance Position Sensing Switch When Tools required Description Hexagonal key 9 64 inch Parts required p n 1 0490 1849 No start signal when switching to the inject position Description Position sensing switch 1 Loosen the three stator screws 2 Remove the stator head 32 1260 Infinity Manual Injector Maintenance 4 iak Position Sensing S 3 Remove the stator ring 5 Insert the new sensing switch into the stator ring 4 Disconnect the sensor cable from the start cable Pull the sensing switch out of the stator ring 6 Install the stator ring Ensure the pin in th
8. Manual Injector 27 Cleaning the Manual Injector 28 Injection Valve Seal 29 Position Sensing Switch 32 Parts and Materials for Maintenance 35 Manual Injector 36 Injection Valve Assembly 38 Appendix 41 Agilent Technologies on Internet 42 1260 Infinity Manual Injector 1260 Infinity Manual Injector Introduction Introduction to the Manual Injector 6 Operation and mechanical hardware ee Agilent Technologies 1 introduction Introduction to the Manual Injector Sample is loaded into the external 20 ul sample loop through the injection port at the front of the valve The valve has a ceramic stator and PEEK injection seal PEEK is compatible with pH 0 14 incompatible with some concentrated mineral acids A make before break passage in the stator ensures flow is not interrupted when the valve is switched between the INJECT and LOAD positions and back again see also Needles on page 21 and Flow Connections on page 14 The valve is mounted on a steel mounting pole and can be installed at the left or right hand side of the LC system Mounting pole Injection port Figure 1 The Manual Injector installed to the mounting pole 6 1260 Infinity Manual Injector R 1260 Infinity Manual Injector e e r a o e 0 e 90 r Installing the Manual Injector ry e Unpacking the Manual Injector Damaged Packaging 8 Delivery Checklist 8 Installing the Manual Injector Flow Connection
9. ct or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1260 Infinity Manual Injector In This Guide In This Guide This manual covers the Agilent 1260 Infinity Manual Injector G1328C 1 Introduction Operation and mechanical hardware 2 Installing the Manual Injector Installation of the manual injector 3 Using the Manual Injector How to use the manual injector 4 Maintenance Instructions on simple routine repair procedures 5 Parts and Materials for Maintenance Detailed illustrations and lists for identification of parts and materials 6 Appendix Additional information 1260 Infinity Manual Injector Contents Contents Introduction 5 Introduction to the Manual Injector 6 Installing the Manual Injector 7 Unpacking the Manual Injector 8 Installing the Manual Injector 10 Flow Connections 14 Leak Drainage 16 Using the Manual Injector 17 Warnings and Cautions 18 Solvent Information 19 Information on Injection Seal Material 20 Needles 21 Injecting Sample 22 Maintenance 25 Overview of Maintenance 26 Flushing the
10. ctor baseplate see Figure 4 on page 12 Figure 4 Installing the System 12 1260 Infinity Manual Injector Installing the Manual Injector APG remote connector Start cable Position sensor cable D00DDD Valve see 1 Figure 5 Installing the Start Cable 2 1 See Figure 6 on page 15 6 Connect the capillaries to the manual injector see Flow Connections on page 14 1260 Infinity Manual Injector 13 2 Installing the Manual Injector Flow Connections WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere CAUTION Prevent siphoning gt The outlets
11. e stator ring is aligned with the hole in the valve body and the position sensoring switch is back in place 1260 Infinity Manual Injector 33 4 Maintenance Position Sensing Switch 7 Insert the stator face onto the stator head 9 Secure the stator head in place with the stator screws Tighten each screw alternately 4 turn until the stator head is secure 34 8 Install the stator head onto the valve Ensure the pin in the stator ring is aligned with the hole in the stator head 1260 Infinity Manual Injector 1260 Infinity Manual Injector r e e e ee 5 7 e Parts and Materials for Maintenance amp o Manual Injector 36 Injection Valve Assembly 38 Detailed illustrations and lists for identification of parts and materials te Agilent Technologies 35 5 Parts and Materials for Maintenance Manual Injector Item p n Description 1 5067 4191 Manual Front Loading Injector Valve 600 bar 2 5001 3738 Mounting pole stainless steel 3 G1328 44121 Base plate 4 5042 8553 Organizer plate 5042 8576 Catch tube cap 5 5043 0208 Name plate 5190 1501 Valve syringe fixed needle 50 pL G1328 87600 Connection capillary 0 17 mm id 500 mm 0100 1677 Start cable Manual injector valve see Injection Valve Assembly on page 38 36 1260 Infinity Manual Injector Parts and Materials for Maintenance 5 Manual Injector Figure 12 Manual Injector 1
12. ith warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the produ
13. nformation on Injection Seal Material The manual injector is supplied with a PEEK injection seal PEEK is compatible with pH 0 14 incompatible with some concentrated mineral acids see Injection Valve Assembly on page 38 20 1260 Infinity Manual Injector Using the Manual Injector Needles CAUTION Needle can damage valve gt Always use the correct needle size 3 Use needles with 0 028 inch outer diameter 22 gauge x 2 inch long needle without electro taper and with 90 point style square tip 1260 Infinity Manual Injector 21 3 Using the Manual Injector Injecting Sample LOAD PositionLOAD Position In the LOAD position see Figure 9 on page 22 the pump is connected directly to the column ports 2 and 3 connected and the needle port is connected to the sample loop At least 2 to 3 sample loop volumes more if better precision is required of sample should be injected through the needle port to provide good precision The sample fills the loop and excess sample is expelled through the vent tube connected to port 6 From Pump To column Needle port connected from rear loop Waste Figure 9 LOAD Position INJECT Position In the INJECT position see Figure 10 on page 23 the pump is connected to the sample loop ports 1 and 2 connected All of the sample is washed 22 1260 Infinity Manual Injector Using the Manual Injector 3 out of the loop onto the column The needle port i
14. o a 20 uL loop With larger than half the loop volume some of the sample is lost out Vent Line 6 This is because sample flows down the center of the loop at twice the average velocity due to the laminar flow effect 1 In INJECT use the Needle Port Cleaner to flush out the needle port with about 1 mL of mobile phase to flush out contamination from the earlier injection This liquid will exit out Vent Line 5 2 Insert the syringe into the needle port You should feel slight resistance as the needle passes through the needle seal before it stops against the stator face 3 Load the sample slowly onto the loop 4 Leave the syringe in and turn the handle to INJECT 24 1260 Infinity Manual Injector 1260 Infinity Manual Injector 4 Maintenance Overview of Maintenance 26 Flushing the Manual Injector 27 Cleaning the Manual Injector 28 Injection Valve Seal 29 Position Sensing Switch 32 Instructions on simple routine repair procedures ee Agilent Technologies 25 4 Maintenance Overview of Maintenance Table2 Overview of Repair Procedures Procedure Typical Frequency Time Required Notes Flushing the injector After using aqueous buffers or 5 minutes See Flushing the Manual salt solutions Injector on page 27 Exchanging the After approximately 10000 to 10 minutes See Injection Valve Seal on page 29 injection valve seal 20000 injections or when the valve performance shows indication of leakage or wear
15. ow Cell Long term operation at pH gt 11 should be avoided Never leave strongly alkaline solutions in the flow cell without flow Solvents Always filter solvents through 0 4 um filters small particles can permanently block filters frits and capillaries Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on e High concentrations of inorganic acids like sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2 CHCly O gt 2 COCl 2 HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol may attack steel Mixtures of carbon tetrachloride with 2 propanol or THF dissolve stainless steel 1260 Infinity Manual Injector 19 3 Using the Manual Injector I
16. r plate 5042 8553 1 Catch tube cap 5042 8576 1 1260 Infinity Manual Injector Installing the Manual Injector 2 Table 1 Manual Injector Checklist Description Quantity Valve syringe fixed needle 50 uL 5190 1501 1 User Manual G1328 90013 1 1260 Infinity Manual Injector 9 2 Installing the Manual Injector Installing the Manual Injector CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions The manual injector can be installed at the left or right hand side of the instrument stack 1 Place the baseplate on the bench 2 Connect the two organizer plates to the base plate Figure 2 Connecting the Organizer Plates 3 Screw the mounting pole into one of the two holes in the organizer plate 10 1260 Infinity Manual Injector Installing the Manual Injector 2 4 Slide the manual injector onto the mounting pole see Figure 3 on page 11 Tighten the mounting screw Mounting screw Figure 3 Installing the Mounting Pole and Manual Injector 1260 Infinity Manual Injector 11 2 Installing the Manual Injector 5 Install other system modules on top of the manual inje
17. s Leak Drainage 8 10 14 16 Installation of the manual injector if Agilent Technologies 2 Installing the Manual Injector Unpacking the Manual Injector Damaged Packaging Upon receipt of your manual injector inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the manual injector has been mechanically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carriers inspection Delivery Checklist Ensure all parts and materials have been delivered with the manual injector The delivery checklist is shown in Table 1 on page 8 To aid in parts identification please see Parts and Materials for Maintenance on page 35 Please report missing or damaged parts to your local Agilent Technologies sales and service office Table 1 Manual Injector Checklist Description Quantity Manual Front Loading Injector Valve 600 bar 5067 4191 1 A 20 uL sample loop is connected between Ports 1 and 4 The valve is supplied with a start cable Hex Keys x2 Mounting Screws x2 Vent Lines x2 Needle Port Cleaner x1 Long Nuts x3 Extra Long Nut x1 Ferrules x4 and a mounting bracket Mounting pole stainless steel 5001 3738 1 Connection capillary 0 17 mm id 500 mm G1328 87600 1 Base plate G1328 44121 1 Organize
18. s and Cautions 18 Solvent Information 19 Information on Injection Seal Material 20 Needles 21 Injecting Sample 22 How to use the manual injector Apg Agilent Technologies 3 Using the Manual Injector Warnings and Cautions Ejection of mobile phase When using sample loops larger than 100 pL mobile phase may be ejected from the needle port as the mobile phase in the sample loop decompresses gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Splashing of solvent gt When using the Needle Port Cleaner empty the syringe slowly to prevent solvent from splashing back at you gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used CAUTION Potential damage to the valve gt Rinse the valve with water after using buffer solutions to prevent crystals from forming which can cause scratches on the rotor seal 18 See Flushing the Manual Injector on page 27 1260 Infinity Manual Injector Using the Manual Injector 3 Solvent Information Observe the following recommendations on the use of solvents Fl
19. s connected to the vent tube port 5 From pump To column Needle port connected from rear Sample loop Waste Figure 10 INJECT Position Complete Loop Filling In complete filling the volume of sample injected is set by the volume of the loop this includes the valve passages This method produces the highest precision At least 2 to 3 sample loop volumes more if better precision is required of sample should be injected through the needle port to provide good precision The sample fills the loop and excess sample is expelled through the vent tube connected to port 6 An excess of sample is needed because mobile phase near the wall of the loop is displaced slowly due to the laminar flow effect 1 Turn the handle to the LOAD position 1260 Infinity Manual Injector 23 3 Using the Manual Injector 2 Insert the syringe into the needle port You should feel slight resistance as the needle passes through the needle seal before it stops against the stator face 3 Load the sample slowly onto the loop Repeat this step for higher precision 4 Leave the syringe in and turn the handle to INJECT Partial Loop Filling If you only have small quantities of sample this is the method of choice In the partialfilling method the volume of sample injected is set by the syringe In this method no more than half a loop volume of sample should be loaded into the loop For example load no more than 10 uL int
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