Centrifuge 5804 / 5804 R / 5810 / 5810 R 1
Contents
1. Centrifuge 5804 5804 R 5810 5810 R Instruction Manual Mode d emploi succinct Instrucciones breves eppendorf Centrifuge 5804 5804 R 5810 5810 R Shortcuts Task Function Lid Press Display Chapter in Centrifuge instruction 5804 R 5810 R manual Parameter set 1 Select 1 Selected a 7 o or dle parameter y flashes E 2 Select 2 New nominal a Or v value appears 1 Press repeatedly Acceleration 3 11 Ta E ramp 9 fast 4 Qo 0 slow a 2 Select ramp A Deceleration ramp 9 0 a v Alarm ON OFF m o A alarm on 3 19 4 alarm off a Programming 1 Set parameter 1 Parameter shown 3 14 only during standstill E 2 Press2 x 2 P symbolizes L first free a program no 3 Storing 3 ok gt 2 sec x 3 12 At set rpm 1 e 4 o E y open gt 4 sec x f Centrifuge 5804 5804 R 5810 5810 R Figure 1 2 rpm a rcf G rad prog rpm rcf oo Y accel N brake min sec Fig 1 Display field and control panel of the 5804 5810 non refrigerated rpm a rcf Q rad C prog rpm rcf ooOlJt c ee Y accel X brake min sec Fig 2 Display field and control panel of the 5804 R 5810 R refrigerated 4 v DO e e Contents Centrifuge 5804 5804 R 5810 5810 R Centrifuge 5804 5804 R 5810 5810 R Operating INSTRUCTIONS esce cene RR n eei e e n nuc e e ar EN T Qn Dn wa2. with full plates with full plates with full plates Maximum load per 50 ml Falcon bucket 144g for two conical tubes incl liquid and form insert Specifications on the rotor 4 x 0 6 kg 2 x 1 010 kg 4 x 1 1 kg 4x 1 4 kg weight for loaded buckets A 4 81 rectangular buckets 4 x 1 22 kg A 4 81 MTP The weight specifications printed on the rotor e g 4 x 0 6 kg for the 4 x 100 ml swing bucket rotor A 4 44 correspond to the overall weight of the rectangular bucket inclusive of adapter test tubes and the contents of each individual position In general commercially available deepwell plates with a 96 well plate format may only be used in the buckets of the A 4 62 MTP on condition that the maximum load does not exceed 380 g and the maximum height of the plate does not exceed 53 mm This restriction also applies to stacked titer plates or culture plates Commercially available deepwell plates or filter plates filter plate stacks in the 96 well plate format may be inserted in the buckets of the A 2 DWP only if their load does not exceed 380 g and their height does not exceed 89 mm These restrictions also apply to stacked titer plates or culture plates Commercially available deepwell plates or filter plates filter plate stacks may be inserted in the buckets of the A 4 81 MTP only if their load does not exceed 380 g and their height does not exceed 60 mm These restrictions also apply
3. Number and diameter of bores 2adaptersfor 3 5mLtesttubes 14 x 11 2 adapters for 1 5 2 mL micro test tubes 12 x 11 2 adapters for 7 mL test tubes 12 x 13 2 adapters for 15mL test tubes 7 x 16 2 adapters for 17 mL test tubes 6 x 17 5 2 adapters for 7 18mL test tubes 4 x 20 2 adapters for 12 30 mL test tubes 2 x 26 2 adapters for 30 50 mL test tubes 1 x 31 Mo BO I 2 adapters for 50 75 mL test tubes 1 x 35 2 adapters for 80 120 mL test tubes 1 x 46 4 spare rubber mats 2 spare clamps Adapters for Falcon test tubes for 100 mL rectangular bucket Number and diameter of bores 2 adapters for 15 mL Falcon test tubes 4 x 17 5 2 adapters for 50 mL Falcon test tubes 1 x 31 4 buckets for 50 mL Falcon test tubes 2 x 31 8 spare adapters for 50 ml Falcon tubes as re order Deepwell plate rotor A 2 DWP with 2 DWP buckets for deepwell plates Deepwell plate bucket with weight category for swing bucket rotor A 2 DWP individual delivery only as a replacement delivery with prior agreement 22637401 22637410 22637428 22637444 22637509 22637525 22637541 22637568 22637584 22637622 22637649 22637681 22637703 22637720 22662503 22662511 22637606 22637665 22637452 22637479 22638564 22638572 7 Ordering information Fixed angle rotors and accessories See brochure for the centrifuges 5804 5804 R 5810 5810 R Fixed angle rotor 6 x 85 mL type F 3
4. rotor is asymmetrically loades The rectangular buckets are sorted according to weight The weight category is printed on the side in the groove e g 86 the last two places in grammes Opposing buckets should be of the same weight category When placing follow up orders please state the weight category This also applies to titer plate buckets Ensure that the grooves are clean before inserting the buckets Unclean grooves and pivots prevent the buckets from swinging freely When working with a rotor which is not completely loaded position the tubes within the buckets in such a way that the rotor pivots are stressed evenly Fig 7 Rotor is symmetrically loaded Fig 8 Not permitted pivots are not stressed evenly rotor is not symmetrically loaded A manual swing test with empty tubes must be carried out if oversized tubes i e longer than 100 mm are used 11 Sunendo Operating 12 3 Operating Permitted loads for the swing bucket rotor Rotor A 4 44 A 2 DWP A 4 62 A 4 81 4 x 100 ml Deepwell 4 x 250 ml 4 x 400 ml plate rotor A 4 62 MTP A 4 81 MTP Maximum load per 310g 630 g 780g 100 ml rectangular bucket adapter tubes adapter tubes adapter tubes Maximum load per 380 g 380 g plate carrier 380 g
5. 20 5 Troubleshooting Centrifuges 5804 5810 and 5804 R 5810 R Error Display Cause Solution No display None No main power connection Check power supply cable Power failure Check main power fuse on the device and in the laboratory clean rotor 200 runs Clean rotor and drum Standby off Centrifuge not used for 8 hours Open lid and then close it again Centrifuge does not no rotor No rotor Mount rotor start up Error in the drive or rotor Switch off the device and switch it on recognition again Lid cannot be None Power failure Bring rotor to a standstill activate opened emergency lid release Lid not closed Press Open Lid latch not engaged Press Open and close lid again completely Close Lid Lid not closed correctly Press down lid Lid does not open Lift Lid Lid does not open automatically Open lid manually Centrifuge shakes IMBAL Rotor not loaded symmetrically Check rotor equipment and rotor load during acceleration ROTOR Rotor not fastened Fasten rotor correctly and switches off Device has been moved or work Place device on stable work surface surface unstable Centrifuge switches SPEED Nominal speed for rotor too high Enter new nominal speed off Error 1 Rotor is not recognized Repeat run If error recurs test with Problem with the rotational speed another rent measuring system Error 2 Imbalance sensor damaged Repeat run Error 3 Problem with the rota
6. A For 5804 R and 5810 R only To avoid damage to the compressor caused by incorrect transportation wait four hours after installation before switching on the device To disconnect the centrifuge from the power supply in the event of an error an emergency switch must be installed away from the centrifuge preferably outside the centrifugation room or next to the exit of this room Place the centrifuge onto a horizontal stable and resonance free work surface Ensure that the working environment is well ventilated and not exposed to direct sunlight There should be 15 cm clearance at the sides of the centrifuge and 10 cm to the rear According to the regulations of the EN 61010 2 020 standard a safety distance of 30 cm must be observed around the centrifuge during operation No objects which cause damage when destroyed must be positioned in this space Before plugging in the centrifuge compare your power supply with the electrical requirements listed on the identifica tion plate The mains cable of the centrifuge may be connected only to a socket with a protective conductor On refrigerated centrifuges 5804 R and 5810 R with a mains voltage of 120 V a distinction is made between two versions depending on requirements the 15 A variant is equipped with a conventional IEC power cable see Fig 3 so that these devices can be operated directly at the lab work station using a conventional socket 120 V 15 A However this ca
7. by the tube manufacturer The use of organic solvents e g chloroform may have an adverse effect on the stability of plastic test tubes Following operation in a cooling room run the centrifuge for 30 minutes in the cooling room until it is warm Alternatively allow it to warm up in a lab for at least three hours but do not plug in the centrifuge in order to prevent damage caused by condensation Rotors are high grade components which are subject to extreme mechanical strain Aluminium rotors are protected against corrosion caused by commonly used laboratory chemicals by means of an electrolytic coating although this protection cannot be fully guaranteed Please ensure that the rotor is protected from mechanical damage Even slight scratches and cracks can cause severe inner damage to the rotor materials which are difficult or impossible to detect with the eye Please avoid using aggressive chemicals with the rotors Such chemicals include concentrated and mild alkalis concentrated acids solutions containing mercury ions copper ions and other heavy metal ions chlorinated hydrocarbons and concentrated saline solutions When handling toxic or radioactive liquids or pathogenic bacteria out of Risk Group Il see World Health Organization Laboratory Biosafety Manual observe national regulations In the event of contamination caused by impurities or aggressive agents the rotor must be cleaned immediately using a neutral cleaning liqui
8. ct oU Re 5 Mode d emploi su cclnct usns rr RR a Cee RE Re Eh ARR RE eee ee A 26 32 Instrucciones Dreves iussu bia cR EE GU a Roc gb man d SURGE Ro cec RE n No part of this publication may be reproduced without the prior permission of the copyright owner Copyright 2005 Eppendorf AG Hamburg Centrifuge 5804 5804 R 5810 5810 R Contents 1 INGOGUCHION Mv Tr 6 1 1 Delivery packages irisi vd ca dh ES 6 1 2 UNA OK s hd Ge S ea 6 1 3 Installing the deVICE i32 sta aes K kk Park ee tone a da fap ataca yrange nies bale OR TR TUR Re 6 2 Safety regulations and applicational limitations esee nmn 8 3 Operating i222 den ar dd e da A rf heed ghee ied h ewecr een coher ete peace a 10 3 1 Operating COntrOlS ii a aD Ru EA ur dua ERA RE ended ead POR Age RE ER aed 10 3 2 Mounting dismounting the rotors llli res 10 3 3 Rotor lid F 45 30 11 cnius ae hee ncm ee hd acm RR CR P Rc ORDRE ADR RD S Rae Rod e ce i BUR Rd 10 3 4 loading the rotors ee Dea iw tb ACER Ae Pod e aps e exequi oerte a e x RR nn 10 3 5 Routine centrifugation with preset time rcf display 00 cee eee 12 3 6 Continuous operation esca iaa dd aha Rej a dae esate wedded onda hs 13 3 7 Short spin centrifugation Short spin aa 13 3 8 Time change during the run ee een hun 14 3 9 Refrigeration for 5804 R 5810 R only aa 14 3 10 Presetiradils e ciu E uuu Ea Rue oe VILE ied a te Dos weeds RU RUD e e iet dul ic
9. recommended can reduce the precision accuracy and life of the centrifuges We do not honor any warranty or accept any responsibility for damage resulting from such actio Fixed angle Angle of Tube Rotor code rotor adapter bore Adapter Swing bucket rotor All Eppendorf rotors are designated I l l l according to a simple logical system which describes the technical specifi F A E 45 mmm 30 SS 1 1 A mmm cations as a uniform series of numbers I I and letters e g 22637207 22662961 22637215 22637223 22637231 22637240 22637258 22637266 22637274 22637002 22662970 22637100 22637126 22636243 22636227 22636260 22638581 22638505 22638521 22638548 22634330 22664166 Upon request Upon request 22662678 n tube adapter bore 4 81 Aerosol tight Max no tubes Max no tubes version adapters adapters 23 M uoneuuojur Buusp4o Ordering information 24 7 Ordering information Centrifuge 5810 Bench top centrifuge max capacity 4 x 400 mL with rotational speed regulation up to 14 000 rom 120 V 60 Hz not including rotor 230 V 50 60 Hz not including rotor Centrifuge 5810 R Refrigerated bench top centrifuge max capacity 4 x 400 mL with rotational speed regulation up to 14 000 rom Temperature range 9 to 40 C 120 V 60 Hz not including rotor 230 V 50 60 Hz not including rotor Other voltages or 60 Hz frequency upon request Rotor stand for all rotors fo
10. 0 11 60 x 2 ml 14 000 16 400 7 5 e e 3 Operating 1 The swing bucket rotor can be delivered without buckets equipped with rectangular buckets as an A 4 81 or with MTP buckets as an A 4 81 MTP whereby the flex buckets especially MTP buckets can be purchased extra in sets of 2 or 4 user friendly change of rectangular buckets or MTP and flex buckets is guaranteed by the use of a common rotor cross 2 The swing bucket rotor can be delivered as A 4 62 loaded with buckets or as A 4 62 MTP loaded with microtest plates User friendly exchange of rectangular buckets or microtest plate buckets is guaranteed by the use of all in one rotor cross 3 A 4 81 is equipped with flex buckets and IsoRack adapters Swing bucket rotor AN Only the combinations of rotor buckets adapter which are recommended by the manufacturer may be used Please check whether all buckets have been inserted correctly and can swing freely For purposes of mechanical stabilization all positions of the rotors A 4 44 and A4 62 A 4 62 MTP must be loaded with the same buckets In contrast the rotor A 4 81 A 4 81 MTP can be equipped with a mix of rectangular buckets and titer plate buckets It is in principle not permitted to use swing bucket rotors with only two rectangular buckets or titer plate buckets inserted Fig 5 Rotor A 4 81 MTP symmetrically Fig 6 Not allowed because the pegs are not evenly loaded loades
11. 120 V 60 Hz 950 W 1650 VV 1300 VV Excess current switch 12 A Excess current switch 18 A Excess current switch 15 A 21 ejep e31uup3 Ordering information 22 7 Ordering information Centrifuge 5804 Bench top centrifuge max capacity 4 x 100 mL with rotational speed regulation up to 14 000 rom 120 V 60 Hz not including rotor 230 V 50 60 Hz not including rotor Centrifuge 5804 R Refrigerated bench top centrifuge max capacity 4 x 100 mL with rotational speed regulation up to 14 000 rpm Temperature range 9 to 40 C 120 V 60 Hz not including rotor 230 V 50 60 Hz not including rotor Other voltages or 50 Hz frequency upon request Captain Eppi Rotor key holder 1 piece Captain Eppi Rotor key holder 10 pieces Rotor stand for all rotors for Centrifuges 5804 5804 R and 5810 5810 R 22622501 22622552 22623508 22623559 22639609 22639625 22639021 Swing bucket rotor and accessories see brochure for further details for Centrifuge 5804 5804 R Swing bucket rotor A 4 44 4 x 100 mL with 4 rectangular buckets of 100 mL 1 rectangular bucket of 100 mL with weight category for individual deliveries please state the existing weight category 2 aerosol tight caps for 100 mL rectangular bucket 4 replacement seals for aerosol tight caps of the rectangular bucket 100 mL Adapters for standard test tubes blood withdrawal systems and micro test tubes for 100 mL rectangular bucket
12. 4 6 38 with rotor lid Spare lid for rotor F 34 6 38 2 adapters for micro test tubes 4 x 11 5 2 adapters for 7 15 mL test tubes 2 x 16 5 2 adapters for 15 18 mL test tubes 1 x 18 5 2 adapters for 20 30 mL test tubes 1 x 26 5 2 adapters for 50 mL test tubes 1 x 29 2 adapters for 50 mL Falcon test tubes 1 x 29 5 2 adapters for 15 mL Falcon test tubes 1 x 17 Fixed angle rotor 30 x 1 5 mL type F 45 30 11 with rotor lid Spare lid for rotor F 45 30 11 Fixed angle rotor 30 x 1 5 mL type FA 45 30 11 aerosol tight with rotor lid of aluminium Spare lid for rotor FA 45 30 11 6 adapters for 0 4 mL centrifuge tubes 6 adapters for 0 5 mL micro test tubes and Microtainers 6 adapters for 0 2 mL PCR tubes PCR strip rotor F 45 48 PCR for 6 x 8 strips 6 x 5 strips 48 x 0 2 mL PCR tubes Drum rotor and accessories see brochure for the centrifuges 5804 5804 R 5810 5810 R Drum rotor T 60 11 with rotor lid for 60 x 1 5 mL 2 mL tubes 7 120 x 0 4 mL tubes with 6 adapters for 1 5 mL 2 mL tubes Adapter for 1 5 mL 2 mL tubes set of 6 Adapter for 0 4 mL tubes set of 6 Accessories for the Centrifuges 5804 5804 R Grease for pivots Standard rotor key Conversion kit for interface for 5804 Conversion kit for interface for 5804 R Tray for condensation water Important note Please use the original accessories recommended by Eppendorf Using spare parts or disposables which we have not
13. AB e e ad tia oe 15 3 11 Centrifugation with Soft start stop e m eens 15 3 12 Dur udgm P CLP 15 3 13 Preset programi CP 15 3 14 Programming das ers ean oleae ede dea are A E pur Sane ted Ena Wiebe See A RE i 16 3 15 Write protection s ssim e puer RE Ee aE a ARE E A ake ae EAE ADR E Eaa a KR RU Ea a hae 16 3 16 Nominal v lue display ii iet d a de r pra a eR A he a edd pr Ra a 16 3 1 7 Automatic rotor recognition 25 odio su dore ER dp bat gea dene dead rad Ea ie 16 3 18 Display of elapsed spin time aaa 17 3 19 Switching on off the warning signal lille 17 3 20 Exiting the Service program aa 17 3 21 Control via serial interface optional 2 0 0 eee I III 17 3 22 Opening the centrifuge in the case of a power failure i aa 17 3 23 Overload cutout switch Fuses teen eee eee 17 4 Maintenance and cleaning 2000 cece eee eee eee 18 4 1 plu ceca a en PUS de a A e A a A a a aE a aai 18 4 2 MB II PME x A AA th e de de a ane ee 18 4 3 The aerosol tight rotor icons odds do dci de 18 4 4 Rotorsterilizatiort naasser sa ema do ek A dc Be E RE a pc NA nds Re Re A I 18 4 5 Glass breakages seu edes eere odo RA MU RM AER CU RR a a Geiger ea Dk e a i ee 19 4 6 Refrigerated centrifuges 0 2 hh hh run 19 4 7 NS AA ebbe dtes wisely jw Gade dh ea dvor aos Gated aa fe andes 19 5 Troubleshooting 5 saa rentre woe a ia whens cael oun LN RR case RUBRUM RR E cd 20 6 Techinical data 5 ia a E do RO h RR 21 7 O
14. SA Tel 1 516 334 7500 Toll free phone 800 645 3050 Fax 1 516 334 7506 E Mail info eppendorf com Application Support Europe International Tel 49 1803 666 789 E Mail support eppendorf com North America Tel 800 645 3050 ext 2258 E Mail support_NA eppendorf com Asia Pacific Tel 603 8023 2769 E Mail support_Asia eppendorf com B 5820 900 105 05 1205 Printed in Germany Printed on chlorine free bleached paper eppendorf is a registered trademark
15. Starter Set 2 IsoRack adapters 2 IsoRacks with lid 2 x 0 C cool packs IsoRack for 0 5 ml and 1 5 ml 2 ml micro test tubes Swing bucket rotor A 4 62 4 x 250 mL with 4 rectangular buckets of 250 mL 1 rectangular bucket of 250 mL with weight category for individual deliveries please state the existing weight category 2 aerosol tight caps for 250 mL rectangular bucket 4 replacement seals for aerosol tight caps 22638602 22638637 22638661 22638670 22638700 22638718 22638726 22638742 22638769 22638921 22638785 22638904 22665201 22638696 22638653 22638807 22638840 22638866 22638882 22639005 22638980 22638998 22510070 22638009 22638025 22638033 22638017 7 Ordering information Adapters for standard test tubes blood withdrawal symptoms and micro test tubes for 250 mL rectangular bucket Number and diameter of bores 2 adapters for 1 5 5 mL test tubes 25 x 11 22638203 2 adapters for 1 5 2 mL micro test tubes 16 x 11 22638220 2 adaptersfor 4 7 mLtest tubes 15 x 13 22638246 2 adapters for 3 15ml test tubes 12 x 16 22638262 2 adapters for 7 17 mL test tubes 12 x 17 5 22638301 2 adapters for 7 18mL test tubes 8 x 20 22638327 2 adapters for 18 30 mL test tubes 4 x 26 22638360 2 adapters for 30 50 mL test tubes 4 x 31 22638386 2 adapters for 50 75 mL test tubes 2 x 35 22638408 2 adapters for 80 120 mL test tubes 1 x 46 22638424 2 adapters for 160 250 mL t
16. ach run When a new rotor is recognized the maximum rotational speed appears in the display for two seconds If the nominal speed set is greater than the maximum speed for the rotor used it is aligned to the maximum speed and the run is interrupted SPEED appears in the display and the run must be restarted The rotational speed and the radius for the rcf value will be reset to the maximum permitted value If a run with a program has been started the program number is set to 0 3 Operating 3 18 Display of elapsed spin time If the Time key and the Prog key are pressed simultaneously the total running time in hours of the centrifuge appears in the display This function can only be selected when the rotor is at a standstill 3 19 Switching on off the warning signal Press the Speed and Time keys simultaneously Alarm on and Alarm off appear after 2 seconds speed alternately in the display 3 20 Exiting the Service program a v If the Service program has been selected accidentally press both arrow keys simultaneously 3 21 Control via serial interface optional All centrifuge functions can be operated via the serial interface RS 232 The appropriate conversion must be carried out by the service department Only devices which have been tested in accordance with IEC 950 may be connected via the serial interface 3 22 Opening the centrifuge in the case of a power failure If the magnetic lid latch cannot be activated
17. ading the rotors The rotors and buckets must always be loaded symmetrically The adapters may only be loaded with the test tubes recommended Differences in the weight of the filled sample tubes should be kept as low as possible in order to prolong the life time of the drive and to minimize running noises caused by imbalance AN Performance data of the rotors On each rotor you will find information concerning the maximum weight of a completely loaded bucket bucket including adapter tubes and liquid or titer plate buckets including titer plate and liquid see also applicational limitations Type max capacity max rotational max 5804 5810 Aerosol tight Designation ml speed rpm Radius 5804 R 5810 R centrifugation max rcf x g cm possible Swing bucket rotors A 4 81 4 x 400 ml 4 000 3 250 18 0 e e A 4 81 19 96 x 2 ml 4 000 2 600 14 6 e A 4 81 MTP 1 16 x MTP Platte 4 000 2 900 15 3 e A 4 62 2 4 x 250 ml 4 000 3 200 18 0 e e A 4 62 MTP 2 16 x MTP Platte 4 000 2 750 15 4 e A 4 44 4 x 100 ml 5 000 4 500 16 1 e e e A 2 DWP 4 x Deepwell Platte 3 700 2 250 14 7 e e Fixed angle rotors F 34 6 38 6x85 ml 11 000 15 550 11 5 e e F 34 6 38 6x85 ml 12 000 18 500 11 5 only 5810 R F 45 30 11 30x2ml 14 000 20 800 9 5 e e FA 45 30 11 30x2ml 14 000 20 800 9 5 e e e F 45 48 PCR 6 x 8er PCR Streifen 12 000 15 350 9 5 e e Drum rotor T 6
18. am data to be used first by pressing the parameter keys and the arrow keys or use the data from the last run The at set rpm functions and the set deceleration ramp can also be saved in a program if necessary Press this key twice gt the first free program no indicated by P appears in the display and flashes The desired free program number 1 9 A Z can be selected Hold down this key for two seconds until ok appears in the display The previously set parameters of Temp Speed Time etc are now saved as a data set 8000 If parameters are modified during the run with a fixed program 0 appears in the PROG field and the user exits the program without it being modified To exit the fixed program call up program 0 or modify the parameters 3 15 Write protection In order to avoid accidental deletion of an existing fixed program the old data set must be deleted while the lid is open prior to renewed assignment of a program number Press this key once gt the program no display flashes select the program no which is to be deleted Press this key within ten seconds until cleared appears 800 8 3 16 Nominal value display All nominal values are displayed when the centrifuge is at a standstill During a run all nominal values can be displayed for 2 5 seconds by pressing one of the parameter keys Temp Speed Time 3 17 Automatic rotor recognition Automatic rotor recognition occurs at the beginning of e
19. because of a power failure the emergency lid release can be activated manually Turn off the main power switch Wait until the rotor has come to a standstill The rotor may continue spinning for up to eight minutes Insert the standard rotor key into the opening in the middle of the front part in the nut underneath and turn counter clockwise This disengages the lid allowing it to be opened Be absolutely sure to remove the rotor key afterwards 3 23 Overload cutout switch Fuses The 230 V and 120 V devices have built in thermal overload switches which function as all pole fuses When the overload protection is actuated these switches over the power switch to OFF but do not switch it on again automatically To turn on the overcurrent protector switch again please turn off the centrifuge for 10 seconds with the mains power switch When the centrifuge is subsequently turned on again the overcurrent protector switch will be automatically reactivated 17 Sunendo Maintenance and cleaning 18 4 Maintenance and cleaning 4 1 Device AN The outside of the centrifuge and the rotor chamber should be cleaned regularly with neutral detergent This is for hygiene purposes as well as to prevent contamination caused by residual contamination The user is responsible for cleaning or decontaminating the centrifuge in the event of contamination caused by high risk substances Open the lid of the centrifuge and disconnect the main powe
20. d This is particularly important for the bores of the fixed angle rotor and for the buckets Please clean your rotor regularly using a neutral cleaning liquid e g Extran neutral or RBS neutral This will protect the rotor and prolong its service life According to EN 55011 the centrifuges 5804 5804 R and 5810 5810 R are Class A products Interference with signal reception can occur in residential areas The operator should take appropriate protective measures 2 Safety regulations and applicational limitations The following rotors and accompanying buckets have a maximum operating life of seven years The date of production is engraved on the rotor in four digit form e g 10 98 October 1998 A 4 44 5804 730 003 A 4 62 MTP 5810 711 002 A 2 DWP 5804 740 009 T 60 11 5804 730 003 F 34 6 38 5804 727 002 A 4 81 5810 718 007 A 4 62 5810 709 008 A 4 81 MTP 5810 725 003 Transparent rotor lids made of PC and PP as well as the PC caps of the rectangular buckets have an operating life of three years The date of production is engraved in the form of a clock RS Contact with organic solvents e g phenol chloroform may have an adverse effect on the transparent polycarbonate caps of the aerosol tight rotors Please check lids of this type regularly for chemical damage or for cracks Cracked caps or caps with a milky discoloration must be replaced immediately A Do not use rotors caps or buckets which have been subjected to chemical or mechan
21. elected rotational speed at set rpm Pressing the START STOP key for longer than 4 seconds while the centrifuge lid is open triggers a switching over to the at set rom function While the key is pressed both triangles of the pictogram are illuminated in blue in an alternating sequence The activated at set rpm function is symbolized by the continuously illuminated upper blue triangle inthe pictogram 7 In order to leave th at set rpm function again and thus to activate the countdown of the centrifugation time directly after switching on the centrifuge press the START STOP key while the centrifuge lid is open The standard setting is selected again after 4 seconds symbolized by the illumination of the lower triangle in the pictogram MN E a 3 13 Preset program Programs can only be preselected when the device is at a standstill OO O Press this key once gt the program no that has been set flashes 0 Data from last run 1 9 A Z Fixed programs select further program numbers Returns to program 0 or leaves the programming mode when the lid is open In order to start the selected program immediately the centrifuge lid must be closed prior to pressing the START STOP key Bunendo Operating 16 3 Operating 3 14 Programming Storing a fixed program only possible when the device is at a standstill It is possible to save a maximum of 35 fixed programs 1 9 A Z Enter the progr
22. er 3 16 Automatic rotor recognition rpm max or 200 rpm The status displays mean rpm max The inserted rotor accelerates to its nominal rotational speed 200 rpm The maximum rotational speed for the short spin run can be set over the arrow keys If the Short key is pressed for longer than three seconds while the centrifuge lid is open the device will switch over to the other status and display it for two seconds The status set last will be maintained 18 Vo Bunendo Operating v e 14 3 Operating 3 8 Time change during the run Press this key during the run not during the braking phase change the duration of the run The time elapsed is taken into account in the new actual value 3 9 Refrigeration for 5804 R 5810 R only 00 0 Standby refrigeration The nominal temperature value can be set from 9 C to 40 C It can be modified during centrifugation Once the nominal temperature value has been reached a deviation greater than 3 C is indicated by a flashing temperature display If the temperature deviates by more than 5 C a periodic warning signal can be heard and the centrifuge switches itself off starts a temperature run at a rotor specific speed for the best possible refrigeration The new preselected temperature is quickly attained in the rotor by cooling FT appears in the PROG field The run is then ended automatically at the arrived at nominal temperature or by pre
23. est tubes 1 x 62 22638441 4 spare rubber mats 22638483 4 spare rubber mats for adapter 22638441 22638459 2 spare clamps 22628467 Adapters for Falcon test tubes for 250 mL rectangular bucket Number and diameter of bores 2 adapters for 15 mL Falcon test tubes 9 x 17 5 22638289 2 adapters for 50 mL Falcon test tubes 3 x 31 22638343 2 adapters for 50 mL Falcon test tubes 4 x 31 22638351 Swing bucket rotor 4 x 100 mL type A 4 44 see 5804 5804 R and brochure Swing bucket rotor A 4 62 MTP with 4 plate carriers 22638041 4 plate carriers for swing bucket rotor A 4 62 MTP for 4 plates or 1 deepwell plate 22638068 1 plate carriers for swing bucket rotor A 4 62 MTP with weight category individual delivery only as a replacement delivery with prior agreement 22638076 Deepwell plate rotor A 2 DWP with 2 DWP buckets for deepwell plates 22638564 Deepwell plate bucket with weight category for swing bucket rotor A 2 DWP individual delivery only as a replacement delivery with prior agreement 22638572 Fixed angle rotors and accessories see 5804 5804 R and brochure Drum rotor and accessories see 5804 5804 R and brochure Accessories for the Centrifuges 5810 5810 R Grease for pivots 22634330 Standard rotor key 22664166 Conversion kit for interface for 5810 Upon request Conversion kit for interface for 5810 R Upon request Tray for condensation water 22662678 Important note Please use the original accessories recommended by Eppe
24. f standby cooling to permanent operation is carried out at own risk and can lead to overheating of the compressor Please empty and clean the tray for condensation water on the right at the bottom of the device on a regular basis AN Remove any condensation water and ice regularly from the rotor chamber using a soft moist cloth Defrosting is recommended for ice removal 3 Operating 3 10 Preset radius d Or O The internal conversion of rotational speed to RCF occurs as a standard with the largest radius A smaller radius can be entered for another adapter Press this key repeatedly until the radius symbol G appears to the left of the SPEED display The radius value flashes change the values entered The new rcf value appears three seconds or 10 s during the stillstand after the rotor radius has been entered 3 11 Centrifugation with Soft start stop If the maximum acceleration deceleration speed level 9 is not desired it is possible to set the speeds in nine different levels v e Press this key repeatedly until the symbol for the acceleration levels appears next to the TIME display Presetting the acceleration level 9 0 Deceleration is set in the same way Y Braking level O corresponds to free deceleration For levels O 8 the symbols appear in the display 3 12 At set rpm The at set rpm function triggers a counting down of the centrifugation time upon achieving the pres
25. humidity Degree of contamination Overvoltage category Standardized interface optional Noise level Dimensions W x D x H 5804 5810 5804 R 5810 R Weight 5804 5810 5804 R 5810 R Electrical data for 120 V model Power supply Maximum power requirement 5804 5810 5804 R 5810 R 20 A version 5804 R 5810 R 15 A version Fuse projection in the device 5804 5810 5804 R 5810 R 20 A version 5804 R 5810 R 15 A version Technical specifications subject to change 5804 5804 R 230 V 50 or 60 Hz 900W 1650W Excess current switch 12 A Excess current switch 12 A 14 000 rpm 20 800 rcf 19 000 Nm 11 000 rpm 19 000 Nm 11 000 rpm 4 x 100 ml 1 2 g ml 2 C to 40 C 15 C to 35 C 75 96 2 Ii RS 232 C lt 65 dB A 466 x 496 x 337 mm D 550 with operating section 634 x 496 x 342 mm D 550 with operating section 55 kg 80 kg 120 V 60 Hz 950 W 1650 W 1300 W Excess current switch 12 A Excess current switch 18 A Excess current switch 15 A 5810 5810 R 230 V 50 or 60 Hz 900 W 1650 W Excess current switch 12 A Excess current switch 12 A 14 000 rpm 20 800 rcf 19 000 Nm 11 000 rpm 23 000 Nm 12 000 rpm 4 x 400 ml 1 2 g ml 2 C to 40 C 15 C to 35 C 75 96 2 I RS 232 C lt 65 dB A 535 x 536 x 345 mm D 608 with operating section 700 x 536 x 345 mm D 608 with operating section 68 kg 99 kg
26. ical damage or which have exceeded their maximum operating life PC Polycarbonate PP Polypropylene suoe Uy pjeuonedde pue suonejnbol Aes Operating 10 3 Operating 3 1 Operating controls See the fold back cover at the front of this manual In the text Qo etc signify keys Buckets include buckets and titer plate buckets 3 2 Mounting dismounting the rotors A summary of the various different rotors can be found in the brochure Clean the motor axle and the rotor bores with a cloth before attaching the rotor When fastening loosening the rotor onto from the motor axle ensure that the temperature of the rotor and the motor axle is between 10 C and 30 C Mount the rotor onto the motor axle and tighten the rotor nut by turning clockwise using the appropriate rotor key supplied To dismount the rotor turn the rotor nut counterclockwise using the rotor key When the rotors are not in the centrifuge please place them in the rotor stand Order no 5804 720 008 Donotcentrifuge using rotors and buckets with visible corrosion or mechanical defects see Chapter 2 Safety regu lations and applicational limitations The Centrifuges 5804 5810 and 5804 R 5810 R have automatic rotational speed limitation 3 3 Rotor lid F 45 30 11 The non aerosol tight rotor lid of the rotor F 45 30 11 is attached by pressing the lid onto the rotor The rotor lid need not be screwed tight 3 4 Lo
27. lass from the rotor the buckets the adapters and the rotor chamber You may need to replace the rubber mats and adapters to prevent further damage Fine splinters of glass may otherwise scratch the surface of the rotors and buckets reducing their resistance to chemicals The air turbulences within the rotor chamber produce a very fine black powder of abraded metal In addition to causing damage to the rotor chamber rotor buckets and adapters the powder also contaminates the samples Check the rotor bores regularly for residues and damage 4 6 Refrigerated centrifuges Clean the refrigeration mesh of the heat exchanger on the rear side of the device with a brush at least twice a year Be sure to first switch off the centrifuge and pull the plug Switch off the centrifuge after use leave the lid open and empty the tray for condensation water situated below the device on the front right hand side 4 7 Return of devices When returning centrifuges please ensure that the devices have been decontaminated and thereby do not present a health risk to our Service staff You will find additional information and a blank of the decontamination confirmation at www eppendorf com Do also consult your laboratory safety officer about a suitable decontamination method Please fill out the decontamination confirmation and place it together with the device when it is to be sent back to Eppendorf 19 Huiuesj gt pue 32ueua3jute A Troubleshooting
28. ndorf Using spare parts or disposables which we have not recommended can reduce the precision accuracy and life of the centrifuges We do not honor any warranty or accept any responsibility for damage resulting from such action M uoneuuojur Buusp4o Eppendorf Offices ASEAN Eppendorf AG Regional Office in Malaysia Tel 60 3 8023 2769 Fax 60 3 8023 3720 E Mail eppendorf eppendorf com my Internet www eppendorf com my AUSTRALIA NEW ZEALAND Eppendorf South Pacific Pty Ltd Tel 61 2 9889 5000 Fax 61 2 9889 5111 E mail Info eppendorf com au Internet www eppendorf com au AUSTRIA Eppendorf AG c o Schott Austria Tel 43 1 29017560 Fax 43 1 290175620 E Mail gilch pGeppendorf de Internet www eppendorf com BRAZIL Eppendorf do Brasil Ltda Tel 55 11 30 95 93 44 Fax 455 11 30 95 93 40 E Mail eppendorf eppendorf com br Internet www eppendorf com br CANADA Brinkmann Instruments Ltd Tel 1 905 826 5525 Fax 1 905 826 5424 E Mail canada brinkmann com Internet www brinkmann com CHINA Eppendorf AG Tel 86 21 68760880 Fax 86 21 50815371 E Mail market info eppendorf cn Internet www eppendorf cn FRANCE EPPENDORF FRANCE S A R L Tel 33 1 30 15 67 40 Fax 33 1 30 15 67 45 E Mail eppendorfGeppendorf fr Internet www eppendorf fr GERMANY Eppendorf Vertrieb Deutschland GmbH Tel 49 2232 418 0 Fax 49 2232 418 155 E Mail vertriebGeppendorf de Internet w
29. r Centrifuges 5804 5804 R and 5810 5810 R 22625004 22625055 22625501 22625551 22639021 Swing bucket rotor and accessories see brochure for further details for Centrifuges 5810 5810 R Swing bucket rotor A 4 81 4 x 400 mL with 4 rectangular buckets of 400 mL 1 rectangular bucket of 400 mL with weight category for individual deliveries please state the existing weight category 2 aerosol tight caps for 400 mL rectangular bucket Replacement cap sealings Adapters for standard test tubes Vacutainers and Falcons for 400 rectangular bucket 2 adapters for 2 6 7 mL 25 x 18 number and diameter of bores 2 adapters for 5 mL Monovette 18 x 13 2 adapters for 7 17 mL 16 x 17 5 2 adapters for 5 mL Falcon 12 x 7 5 2 adapters for 50 mL Falcon 5 x 31 2 adapters for 180 250 mL tubes 1 x 62 2 adapters for 400 mL 1 x 81 2 adapters for Centriprep Centrifugal Filter Units 5 x 31 Replacement adapter rubber mats Replacement adapter clamps 2 bottles 400 mL Swing bucket rotor A 4 81 MTP with 4 MTP Flex buckets 4 MTP Flex buckets for swing bucket rotor type A 4 81 MTP or A 4 81 for use of IsoRack adapters cell culture flasks adapters and MTP DWP 2 MTP Flex buckets 1 MTP Flex bucket 2 adapters for each cell culture flask for use in the MTP Flex buckets 2 adapters for each IsoRack for 0 5 ml micro test tubes 2 adapters for each IsoRack for 1 5 and 2 0 ml micro test tubes 1 IsoRack
30. r plug Unscrew the rotor with the rotor key provided and clean separately Only neutral agents may be used for cleaning and disinfection e g diluted Extran neutral RBS neutral or 70 isopropanol water mixture or an alcohol based disinfectant The rotor chamber should only be cleaned with a moist cloth After cleaning with detergent the rubber seals in the rotor chamber should be rinsed well with water and lubricated with glycerine in order to prevent the seals from becoming brittle If condensation water forms in the rotor chamber after freezing occurs dry with a soft absorbent cloth The user must consult the manufacturer before cleaning or decontaminating the centrifuge using methods not recommended by the manufacturer in order to ensure that the centrifuge and accessories are not damaged To ensure that the centrifuge functions correctly and safely in the long term please note that aggressive chemicals can damage the rotor buckets and boiler Please check the centrifuge regularly for damage caused by corrosion 4 2 The rotors The rotor and buckets must be cleaned regularly to prevent contamination caused by residue Check at least the rotor and housing monthly for residue and corrosion This applies in particular to the rotor bores Please clean your rotor using a neutral cleaning liquid This will protect the rotor and prolong its service life As a reminder the message clean rotor appears in the display of the centrifuge three time
31. rdering information 5 2 E er E E as 22 s u231u0 Introduction 1 Introduction The Centrifuges 5804 5810 are non refrigerated bench top centrifuges and the Centrifuges 5804 R 5810 R are refrigerated bench top centrifuges The 5804 5804 R models have a maximum capacity of 400 ml and the 5810 5810 R models have a maximum capacity of 1600 ml All centrifuges are intended for use in research and routine laboratories in the biosciences and in the fields of medicine and research This sign is found on your centrifuge and on several pages in the operating manual Texts labeled with this sign contain safety notes Read these safety precautions before using the centrifuge for the first time Before starting up the Centrifuges 5804 5810 and 5804 R 5810 R for the first time please read the instruction manual 1 1 Delivery package 1 Centrifuge 5804 5810 with fan cooling or 1 refrigerated Centrifuge 5804 R 5810 R rotor not included with centrifuges 1 Main power cable European standard plug 1 Instruction manual 1 Rotor key 1 2 Unpack When the machines are removed from the packaging they must be supported by two persons near the rubber feet at the bottom on the left and right hand side and then carried the short distance to the lab bench Please observe the weight bearing capacity of the bench If the machines are to be transported over long distances in the lab a trolley must be used 1 3 Installing the device
32. s after every 200 runs When using the swing bucket rotor ensure that the grooves in which the buckets are fitted are free of contamination The buckets can be lubricated with the lubricant grease for pivots supplied although care must be taken to ensure that the buckets can still swing freely 4 3 The aerosol tight rotor The sealing rings of the aerosol tight rotor FA 45 30 11 are subject to natural wear and tear and should be replaced regularly if damaged To protect the rotor please ensure that the sealing rings are maintained regularly When handling the rotor lid please observe the specifications regarding the chemical resistance of the materials of construction The cover of the aerosol tight rotor must not be fastened tightly during storage 4 4 Rotor sterilization All rotors are autoclavable 121 C 20 min After the rotor has been autoclaved ten times the lids of the aerosol tight buckets or rotors must be exchanged The aerosol tight Rotor FA 45 30 11 and the lid can be autoclaved at 142 C for 2 hours to destroy prions but the aerosol tight lid must be exchanged after each autoclaving 4 Maintenance and cleaning 4 5 Glass breakage When centrifuging glass tubes please observe that high speeds rcf s increase the risk of glass breakage Please follow the manufacturer s instructions concerning the maximum speed rcf of centrifuge tubes In the event of glass breakage carefully remove all splinters and all ground g
33. s to the nominal speed During the run the rotational speed of the rotor or the appropriate rcf value the sample temperature and the remaining spin time in minutes are displayed The last minute is counted down in seconds During the run all parameters can be modified After the end of a run or after a run has been interrupted by pressing the Stop key the rotor is braked and brought to a standstill During the braking process the time display flashes and the elapsed spin time is displayed When the lamp in the Open key lights up the lid can be unlocked by pressing the key 3 6 Continuous operation The continuous operation function is set above 99 min or below 1 min using the arrow keys In the time ime display oo indicates continuous operation During the run the actual rotational speed RCF and temperature values and the elapsed time in minutes are displayed If the centrifuge runs for more than 99 min 99 appears in the display The run is ended with the STOP key 3 7 Short spin centrifugation Short spin Press for as long as the run should last The text SH will appear in the display The elapsed time is displayed in seconds An already interrupted run can be continued up to two times by repeatedly pressing the key as long as the centrifuge has not yet come to a stillstand If the Short key is pressed while the lid is open one of the following two status signs will appear depending upon the rotor recognized see Chapt
34. ssing the Stop key A signal can be heard at regular intervals The centrifuge does not terminate the run until the rotor carrier or rectangular bucket have reached the pre selected temperature The target temperature is initially measured on the chamber and then shown in the display However the temperature run is automatically terminated when the rotor carrier or rectangular bucket have reached the target temperature When the lid is closed the rotor chamber is refrigerated to the preselected nominal temperature before or after a run as long as this value is below ambient temperature The rotor does not turn during this procedure and the temperature changes more slowly If the centrifuge is not used for longer than 8 hours or if the lid is not opened for this time the refrigeration function switches off for safety reasons Standby off appears in the display together with the nominal temperature in the rotor chamber The desired temperature can be reached rapidly via the Fast Temp function The standby cooling of the centrifuge can also be switched on permanently To do this the Temp and Prog keys must be pressed simultaneously Standby 8h appears in the display Following this the Fast temp must be pressed immediately in order to switch the standby cooling over into permanent operation Standby endless appears in the display The standby cooling is automatically switched off after 8 hours in order to protect the device The switching o
35. tional speed Leave device switched on for 8 min press measuring system Open then open the device Repeat run Error 4 Lid latch sensor damaged Switch device off and then switch it on again Repeat run Error 5 Unauthorized opening of lid or lid Repeat run switch damaged overload Converter overloaded Allow to cool Restart device after 5 min or Brake defective Error 6 Voltage supply too low Check voltage supply Rotor becomes loose Tighten rotor Error 7 Overspeed Repeat run Error 8 Rotor loose Tighten rotor Drive error Motor defective Centrifuge switches overtemp Temperature deviation from Repeat run off Warning signal nominal value 5 C Error 9 25 Electronics error Clear Memory Program memory full Delete several programs or press Start or Prog to begin new run Interrupt Power failure during the run Restart Flashing temperature display Temperature deviation from nominal value gt 3 C If the above solutions are unsuccessful please contact SERVICE 6 Technical data Centrifuges Power supply Maximum power requirement 5804 5810 5804 R 5810 R Fuse protection 5804 5810 5804 R 5810 R Max rotational speed Max centrifugal force Max kinetic energy 5804 5810 5804 R 5810 R Max load Max density of material to be centrifuged Permitted ambient temperature during operation 5804 5810 5804 R 5810 R Permitted maximum relative air
36. to stacked titer plates or culture plates If deepwell plates used with dimensions which do not correspond to the values stated above e g prototypes combination plates with a heavy lid or specially designed plates the user must consult Eppendorf to establish the suitability of using these plates in a titer plate or Deepwell plate rotor Under no circumstances use MTP MTP Flex and DWP buckets with deformed side plates 3 5 Routine centrifugation with preset time rcf display Turn on the main power switch The nominal values of the last run are displayed Load the rotor symmetrically and close the centrifuge lid The Open key lights blue d activates the preset speed The SPEED display flashes modify the values The new nominal value appears in the display If the preset speed is in rcf please check the radius that has been entered see Section 3 10 x e 3 Operating to select rcf press this key repeatedly until the rcf symbol appears to the left of the SPEED display speed The rcf value flashes and can be modified using the arrow keys activates the preset time The TIME display flashes The time can be modified using the arrow keys activates the preset temperature The nominal temperature in C can be modified using the arrow keys starts the run lll flashes and displays the rotation of the rotor 0600 Rotor recognition see also Chapter 3 16 occurs at 200 to 700 1 min and the device then accelerate
37. uses a drop in the cooling performance of this kind of device This technical specification leads to a rise in the lowest temperature which can be reached at maximum speed and to slower cooling down to the specified value 1 Introduction Fig 3 15 A IEC power cable Fig 4 20 A standard variant On the 20 A standard variant by contrast the power cable is permanently attached to the device see Fig 4 These devices have a special 20 A plug and require the appropriate socket to guarantee the supply of current and voltage required for the centrifuges 120 V 20 A The advantage is greater more rapid cooling To switch on the centrifuge press the mains switch on the right hand side of the device The nominal values of the test run appear in the display and the control lamp in the Open key lights up The centrifuge lid can be opened by pressing the Open key Please insert the rotor before starting and tighten it with the supplied rotor key The rotors A 4 81 and A 4 81 MTP are tightened with the special rotor key included in the rotor delivery package uononpoaju Safety regulations and applicational limitations 2 Safety regulations and applicational limitations In the interests of your own personal safety always observe the following regulations The rotor and the rotor cover must always be securely fastened Do not begin centrifugation before the rotor has been securely fastened The rotor must be loaded symmetricall
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39. y Opposing tubes should be of the same type and should be filled equally For reasons of mechanical stabilization all positions of rotor A 4 44 and A 4 62 A 4 62 MTP must be loaded with buckets of the same type Prior to centrifugation the tubes should in any case be visually inspected for material damage Damaged tubes may not be centrifuged This is because broken tubes can in addition to sample loss result in further damage to the centrifuge and accessories Do not use buckets and rotors which show clear signs of corrosion or mechanical defects Please check accessories at regular intervals Do not operate centrifuges which have not been correctly installed or repaired Do not move or knock against the centrifuge during operation Repairs must only be performed by an Eppendorf authorized service technician Only use original rotors and spare parts recommended by Eppendorf The Centrifuges 5804 5810 and 5804 R 7 5810 R may only be used for special applications They must not be operated in a hazardous or flammable environment and must not be used to centrifuge explosive or highly reactive substances If such liquids are spilled in the rotor or rotor chamber the centrifuge must be cleaned with a moist cloth and a mild soap solution A liquid density of 1 2 g ccm must not be exceeded at the maximum rotational speed During longer spin times in the 5804 5810 models the sample tubes may heat up Observe the limiting data specified
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