JA-12 Fixed Angle Rotor Manual
Contents
1. Also ensure that O rings are lightly but evenly coated with silicone vacuum grease 335148 2 Load the filled containers symmetrically into the rotor Refer to TUBES on page 15 for information about containers If fewer than twelve tubes are being run they must be arranged symmetri cally in the rotor see Figure 3 Opposing tubes must be filled to the same level with liquid of the same density Figure 3 Typical Examples of Arranging Tubes in the Rotor OPERATION INSTALLING THE ROTOR JA 12 Rotor e Precool the rotor in the centrifuge or in a refrigerator before use especially before short runs to ensure that the rotor reaches the set temperature A suggested precooling cycle is a minimum of 30 minutes at 2000 rpm at the required temperature Ifyou are using a microprocessor controlled centrifuge Avanti J series and some J2 models be sure to enter rotor code 12 Refer to your centrifuge instruction manual for additional information During acceleration in an Avanti J series centri fuge the JA 12 rotor may be misidentified as a JA 14 rotor If such misidentification occurs it is possible to set the run speed to 14 000 rpm and operate the rotor at that speed To prevent misidentification always select the JA 12 rotor and a maximum speed of 12 000 rpm during run setup and do not change these settings Do not run the JA 12 rotor above 12 000 rpm at any time or labware failure may result Rotor Drive Pin2. observe the maximum speed limits and fill volumes shown Maximum fill volume is the maximum amount that can be centrifuged in the container listed Refer to Appendix A in Rotors and Tubes for information on the chemical compatibilities of tube and accessory materials The tubes listed in Table 3 should be used one time only Tube damage and sample leakage will result from repeated use Do not use glass tubes in this rotor 15 JA 12 Rotor 16 Temperature Limits e Plastic containers have been centrifuge tested for use at temperatures between 2 and 25 C For centrifugation at other temperatures pretest containers under anticipated run conditions e Ifplastic containers are frozen before use make sure that they are thawed to at least 2 C prior to centrifugation Table 3 Tubes for the JA 12 Fixed Angle Rotor Manufacturers Nominal Nominal Max Fill Maximum Dimensions Volume Volume Recommended Manufacturer Description mm mL mL g force for tube Corning Polypropylene capped 30 x 115 50 45 9400 x g Falcon Polypropylene capped 30 x 115 50 45 9400 x g Sarstedt Polypropylene capped 29 x 115 50 40 8800 x g The JA 12 rotor generates g forces higher than the tube manufact urers recommended maximums see Table 3 Running these tubes above the recommended maximum g forces will cause white striations known as crazing see Figure 8 Moderate crazing will not affect run resu
3. rotational speeds higher than the maximums you have determined to be appropriate for your protocols in no case above 12 000 rpm When solutions more dense than 1 2 g mL are centrifuged in this rotor use equation 7 to calculate the reduced maximum allowable rotor speed 1 2 g mL 7 reduced maximum speed pa p P density of tube contents where p maximum speed CARE AND MAINTENANCE MAINTENANCE Dual locking Rotor Lid 364846 a _ Small O ring Large O ring 961697 961696 Check for Corrosion Y Rotor Body I gt NOTE Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use e Periodically at least monthly inspect the rotor especially inside cavities for rough spots or pitting white powder deposits frequently aluminum oxide or heavy discoloration If any of these signs are evident do not run the rotor Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center e Before using the rotor inspect the rotor drive pins to ensure that they are not damaged Damaged drive pins can prevent the rotor from seating properly on the centrifuge drive hub Avanti J series centrifuges only To inspect the drive pins turn the rotor upside down and look into the drive hole in the center of the rotor If the drive
4. COULTER 2009 Beckman Coulter Inc J TB 051AG July 2009 JA 12 Fixed Angle Rotor Used In Beckman Coulter J2 and Avanti J Series Centrifuges JA 12 Rotor Pees lee ieee SAFETY NOTICE This safety notice summarizes information basic to the safe use of the rotor described in this manual The international symbol displayed above is a reminder to the user that all safety instructions should be read and understood before operation or maintenance of this equip ment is attempted When you see the symbol on other pages throughout this publication pay special attention to the specific safety information presented Observance of safety precau tions will also help to avoid actions that could damage or adversely affect the performance of the rotor This rotor was developed manufactured and tested for safety and reliability as part of a Beckman Coulter centrifuge rotor system Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter s manufacture or in a Beckman Coulter centri fuge that has been modified without Beckman Coulter s approval Handle body fluids with care because they can transmit disease No known test offers complete assurance that such fluids are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other infectious samples accor
5. Orientation Two metal pins inside the rotor drive hole engage with teeth on the centrifuge drive hub to prevent the rotor from slipping during acceler ation and deceleration see Figure 4 When the rotor is properly installed the pins sit either in the drive hub grooves or on top of the hub surface Figure 5 In rotors manufactured since early 1997 the rotor pins are positioned parallel to the BECKMAN name engraved at the center of the rotor body see Figure 6 Knowing the pin orientation before you install the rotor will help to ensure that you position the rotor properly on the hub minimizing the chance of hub damage Follow the steps below JA 12 Rotor SECKMAKy Rotor Drive Hole Drive Pins Avanti J Centrifuge Drive Spindle Hub Figure 4 Rotor Drive Pin Location and Orientation Drive Rotor Hub Pins 2 Teeth 2 Grooves 4 Drive Hub Drive Hub es Drive i Drive Spindle Spindle Assembly Assembly Older Model Centrifuges Newer Model Centrifuges Be sure the pins in the rotor drive hole Be sure the pins in the rotor drive hole are seated in the grooves are not sitting on top of the teeth of the drive spindle hub as shown on the drive spindle hub Figure 5 Centrifuge Drive Hub Configurations JA 12 Rotor Top View Side View Figure 6 Installing the Rotor Rotor Lid Configurations The rotor has two available l
6. ack of this manual for warranty information iim C2 D gt Annulus ae Figure 2 Fluid Containment Annulus PREPARATION AND USE Specific information about the JA 12 rotor is given here Use the Rotors and Tubes for Beckman Coulter J Series Centrifuges manual publication JR IM and the centrifuge instruction manual along with this manual for complete rotor and accessory operation I gt NOTE Although rotor components and accessories made by other manufacturers may fit in the JA 12 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the JA 12 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor JA 12 Rotor PRERUN SAFETY CHECKS ROTOR PREPARATION Read the Safety Notice page at the front of this manual before using the rotor 1 Make sure that the rotor and lid are clean and show no signs of corrosion or cracking 2 Refer to Appendix A in Rotors and Tubes for the chemical compatibilities of all materials used 3 Verify that the tubes being used are listed in Table 3 For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration 1 Be sure that metal threads in the rotor are clean and lightly but evenly lubricated with Spinkote lubricant 306812
7. ase If the rotor and or accessories becomes contaminated with radioac tive material it should be decontaminated using a solution that will not damage the anodized surfaces Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum RadCon Surface Spray or IsoClean Solution for soaking and Radiacwash IlIi NOTE IsoClean can cause fading of colored anodized surfaces Use it only when necessary and remove it promptly from surfaces While Beckman Coulter has tested these methods and found that they do not damage components no guarantee of decontamination is expressed or implied Consult your laboratory safety officer regarding the proper decontamination methods to use If the rotor or other components are contaminated with toxic or patho genic materials follow all appropriate safety and decontamination procedures as outlined by your laboratory safety officer 3 In U S contact Nuclear Associates New York in Eastern Europe and Commonwealth States contact Victoreen GmbH Munich in South Pacific contact Gammasonics Pty Ltd Australia in Japan contact Toyo Medic Co Ltd Tokyo 4 In U S contact Biodex Medical Systems Shirley New York internationally contact the U S office to find the dealer closest to you 22 JA 12 Rotor STERILIZATION AND DISINFECTION The rotor and all rotor components can be autoclaved at 121 C for 401 C up to an hour Remove the lid from th
8. cement price Current rotor list price x CONDITIONS 1 Except as otherwise specifically provided herein this war ranty covers the rotor only and Beckman Coulter Inc shall not be liable for damage to accessories or ancillary supplies including but not limited to i tubes ii tube caps iii tube adapters or iv tube contents 2 This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance con trary to the instructions in the Beckman Coulter rotor or centrifuge manual 3 This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor charac teristics or is operated in a Beckman Coulter centrifuge that has been improperly disassembled repaired or modified DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT ABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CON SEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT B BECKMAN COULTER Beckman Coulter Inc 250 S Kraemer Blvd Brea California 92821 Sales and Service 1 800 742 2345 Internet www beckmancoulter com 2009 Beckman Coulter Inc All rights reserved
9. d to enter the temperature uty Lu F Set 2 Find the compensation value in Table that corresponds with the set temperature and run speed 3 Press COMP ADJ The word COMP flashes below the TEMPERATURE display and the display flashes 12 JA 12 Rotor 4 Use the keypad to enter the required compensation value Press the key to enter a minus sign pressing it again will remove the minus sign 5 Check the temperature display If the entry is incorrect press ce and reenter the digits 6 When the entry is correct press ENTER RECALL gt NOTE To clear a COMP ADJ entry press COMP ADJ 0 and ENTER RECALL REMOVAL AND SAMPLE RECOVERY A CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply all appropriate safety and decontamina tion procedures to the centrifuge and accessories Dual locking Lid 1 Place one hand on the rotor to keep it from turning Grasp the round tie down knob with your other hand and turn the knob to the left counterclockwise until the stem disengages from the drive x hub threads 2 Hold the knob or place your hands on the rotor and lift the rotor straight up off the drive hub 3 Place the rotor on a counter or bench top under a safety hood 4 Grasp the daisy knob and turn it to the left counterclockwise until
10. ding to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol contain ment Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials in nor handle or store them near the centrifuge Although rotor components and accessories made by other manufacturers may fit in the JA 12 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the JA 12 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and acces sories listed in this publication should be used in this rotor Do not run an empty rotor Place filled tubes in at least two opposing cavities Make sure that filled containers are loaded symmetrically into the rotor and that opposing tubes are filled to the same level with liquid of the same density If disassembly reveals evidence of leakage you should assume that some fluid escaped the r
11. e rotor and place the rotor and lid in the autoclave upside down tT Ethanol 70 or hydrogen peroxide 6 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discoloration of anod ized surfaces Use the minimum immersion time for each solution per laboratory standards Qmm While Beckman Coulter has tested these methods and found that they do not damage the rotor or components no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfec tion is a concern consult your laboratory safety officer regarding proper methods to use Refer to publication IN 192 included in each box of tubes or bottles for tube and bottle sterilization and disinfection procedures STORAGE When the rotor is not in use store it in a dry environment not in the instrument with the lid removed to allow air circulation so moisture will not collect in the tube cavities 5 Flammability hazard Do not use in or near operating centrifuges 23 JA 12 Rotor 24 RETURNING A ROTOR Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc This RGA form which may be obtained from your local Beckman Coulter sales office should con
12. fect at the time of sale Beckman Coulter Inc agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within seven 7 years after delivery of a J series rotor to the original buyer by Beckman Coulter Inc or by an authorized represen tative provided that investigation and factory inspection by Beckman Coulter Inc discloses that such defect developed under normal and proper use Should a Beckman Coulter cen trifuge be damaged due to a failure of a rotor covered by this warranty Beckman Coulter will supply free of charge all cen trifuge parts required for repair REPLACEMENT Any product claimed to be defective must if requested by Beckman Coulter Inc be returned to the factory transporta tion charges prepaid and will be returned to Buyer with the transportation charges collect unless the product is found to be defective in which case Beckman Coulter Inc will pay all transportation charges A defective rotor will be replaced by Beckman Coulter Inc at its then current list price less a credit based upon the age of the rotor years since date of purchase The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter s Indianapolis Indiana facility or delivered to a Beckman Field Service representative The replacement price cost to Buyer for the respective rotor shall be calculated as follows years Repla
13. id configurations The dual locking lid 364846 allows the lid to be attached to and removed from the rotor outside the centrifuge so that tubes can be loaded and unloaded under a safety hood The dual locking lid consists of a daisy knob that attaches the lid to the rotor body and a tie down knob that attaches the rotor to the centrifuge Tie down Knob Daisy Knob drive hub Lubricate Threads _7 Tie down Knob The single locking lid 360991 has a tie down knob that secures the rotor to the centrifuge drive spindle The rotor must be placed in the centrifuge before the lid is attached Lubricate ee Threads JA 12 Rotor 10 Rotor Installation To prevent the rotor from sticking to the centrifuge drive hub apply a thin coat of Spinkote lubricant to the rotor drive pins and to the lid knob threads Lightly coat the large and small O rings with silicone vacuum grease Dual locking lid only Place filled tubes into the rotor then place the lid on the rotor and turn the daisy knob to the right clockwise until secure Do not overtighten Turn the rotor until the BECKMAN name is either horizontal or perpendicular to the drive hub pins or teeth see Figure 4 Or rotate the drive spindle to align the hub pins or teeth with the rotor drive pins Carefully lower the rotor straight down onto the centrifuge dr
14. ides contact with the Field Rotor Inspection Program and the rotor repair center Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred Do not allow corrosive materials to dry on the rotor Under normal use wash the rotor frequently at least weekly to prevent buildup of residues 1 Remove the O rings before washing 2 Wash the rotor and lid in a mild detergent such as Beckman Solution 555 that won t damage the rotor The Rotor Cleaning Kit 339558 contains two special plastic coated brushes and two quarts of Solution 555 for use with rotors and accessories Dilute the detergent 10 to 1 with water gt NOTE Do not wash rotor components in a dishwasher Do not soak in detergent solution for long periods such as overnight 3 Thoroughly rinse the cleaned rotor and components with distilled water 4 Air dry the rotor and lid upside down Do not use acetone to dry the rotor 5 Apply a thin even coat of silicone vacuum grease to both O rings before replacing them 21 JA 12 Rotor DECONTAMINATION Clean metal threads every 6 months or as necessary Use a brush and concentrated Solution 555 Rinse and dry thoroughly then lubricate lightly but evenly with Spinkote to coat all threads Periodically remove the O rings and wipe clean as necessary Clean the O ring grooves with a cotton tipped swab Reapply a light film of silicone vacuum gre
15. if intermediate values are required Rotor Required Sample Temperature C Speed rpm 20 10 2 5 10 20 40 12 000 N 6 6 7 7 6 9 10 000 3 4 5 5 5 5 9 5 000 1 3 3 4 4 5 9 An N indicates that the rotor cannot achieve the required temperature at this speed JA 12 Rotor 11 JA 12 Rotor Table 2 Temperature Compensation Settings for the Model J2 HC Analog Centrifuge Interpolate if intermediate values are required Rotor Required Sample Temperature C Speed rpm 20 10 2 5 10 20 40 12 000 N 3 2 2 2 2 1 10 000 2 2 1 1 1 1 1 5 000 1 1 0 0 0 0 0 An N indicates that the rotor cannot achieve the required temperature at this speed Analog J2 Series Centrifuges Models J2 HS and J2 HC Ser 1 Turn the SET knob on the centrifuge control panel to the required O temperature 2 Find the compensation value in Table 1 for Models J2 HS or SOMPE Table 2 for Model J2 HC that corresponds to the required temper ature and run speed Set the COMP dial to that setting Interpolate if intermediate values are required IIIi NOTE Temperature settings for earlier model centri fuges must be empirically determined Microprocessor Controlled J2 Series Centrifuges Models J2 MI and J2 MC TEMPERATURE C 1 Press the TEMP key on the centrifuge control panel and then use the keypa
16. ime from maximum speed rotor fully loaded max brake 0 0000 eee ee 4 min Rotor weight maximum sample mass 11 kg 24 Ib Rotor mat tiall oi 22s isdn da dca iieteta se beaded aluminum Rotor entry code for microprocessor controlled instruments 12 The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass Passing through the critical speed range is characterized by some vibration Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed r to the standard acceleration of gravity g according to the following formula RCF 2 amp where r is the radius in millimeters is the angular velocity in radians per second 2 z RPM 60 and g is the standard acceleration of gravity 9807 mm s After substitution BEM RCF 1 12r ES JA 12 Rotor DESCRIPTION This rotor has been manufactured in a registered ISO 9001 or 13485 facility for use with the specified Beckman Coulter centrifuges ete The JA 12 fixed angle rotor rated for 12 000 rpm is designed to hold 364846 up to twelve 50 mL tubes at a 35 degree angle see Figure 1 Used l in Beckman Coulter J2 and Avanti J series centrifuges the JA 12 pepe i rotor develops centrifugal forces that can efficiently pellet cells aiae bacteria and food products separate proteins viruses and sub O ring cellular fract
17. ions and do phase separation and binding studies 961697 The JA 12 rotor with dual locking lid was tested to demonstrate a eer containment of microbiological aerosols under normal operating conditions of the associated Beckman Coulter centrifuge when used and maintained as instructed The rotor is also available with a single locking lid The JA 12 rotor has a patented fluid containment annulus located below the O ring sealing surface see Figure 2 The annulus helps to retain liquid that may escape from leaking or overfilled tubes thereby minimizing the escape of liquid into the instrument chamber during centrifugation Single locking Lid Dual locking Lid ee g g g Y N WI A Se Y l O iio ZB Figure 1 The JA 12 Fixed Angle Rotor Validation of microbiological containment was done at an independent third party testing facility CAMR Porton Down UK or USAMRIID Ft Detrick MD U S A Improper use or maintenance may affect seal integrity and thus containment JA 12 Rotor The rotor and lid are made of anodized aluminum Two O rings made of ethylene propylene rubber one in the rotor lid and one in the rotor body help to maintain atmospheric pressure inside the rotor during centrifugation when they are properly lubricated Pins in the rotor drive hole mate with the centrifuge drive hub to prevent the rotor from slipping during acceleration and deceleration See the Warranty at the b
18. it disengages 5 Remove the lid and set it aside 13 JA 12 Rotor 14 Single locking Lid 1 Unscrew the tie down knob 2 Remove the lid and set it aside 3 Lift the rotor straight up off the drive hub If the Rotor Sticks to the Centrifuge Drive Hub If the rotor sticks to the centrifuge drive hub use the removal tool 360998 to release it as follows see Figure 7 Rotor Removal Tool 360998 er ELF Figure 7 Using the Rotor Removal Tool I gt NOTE This procedure requires you to remove the rotor lid while the rotor is in the centrifuge If you are using the dual locking lid the biosafe seal provided by this mechanism will be broken when the lid is removed Consult your labora tory safety officer for the proper safety procedures to follow in this case TUBES JA 12 Rotor 1 Remove the lid 2 Screw the removal tool into the threaded opening As the tool tightens down it will push against the drive hub forcing the rotor up and off the hub 3 Lift the rotor up and out of the centrifuge by the removal tool handle or by holding the rotor with your hands 4 Unscrew the removal tool and remove it from the rotor gt NOTE Never run the rotor with the removal tool in place Lubricate the centrifuge drive hub with a light coat of Spinkote to prevent further sticking The JA 12 rotor uses the tubes listed in Table 3 Be sure to use only those items listed and to
19. ive spindle hub Do not drop the rotor onto the spindle hub Slowly turn the rotor around the drive spindle to make sure that it is properly seated In rare cases the rotor pins can rest on top of the spindle hub pins or teeth which prevents the rotor from being secured to the drive spindle Turning the rotor after installing it will move the pins into proper position Secure the rotor to the centrifuge drive spindle as follows Dual locking lid press the lid knob down and turn it to the right clockwise until secure no more than two full turns Do not overtighten Single locking lid place filled tubes into the rotor then place the lid on the rotor Press the lid knob down and turn it to the right clockwise until secure Do not overtighten TEMPERATURE TEMP C To ensure that the JA 12 rotor reaches the required temperature during the run follow the appropriate instructions below for the centrifuge being used Avanti J Series Centrifuges Enter the temperature according to the instructions in your centrifuge instruction manual No additional input is required J2 Series Centrifuges Enter the required temperature and the appropriate temperature compensation units see Tables 1 and 2 on the centrifuge control panel as follows Table 1 Temperature Compensation Settings for Analog and Microprocessor Controlled Centrifuges Models J2 HS JS 21 J2 MI J2 21M J2 MC and J2 21M E Interpolate
20. lts or cause tube leakage However repeated tube usage and or long run times will eventually lead to tube failure To minimize the possibility of tube failure observe the following conditions e Do not use tubes more than once e Discard crazed tubes Do not allow the run length at maximum speed to exceed 30 min e Refer to Table 4 to find the g forces produced at various speeds in the rotor and limit run speed to the speed that produces the maximum recommended g force for the tube in use Beckman Coulter tests indicate that the Sarstedt tubes exhibit more crazing than the Corning or Falcon and that due to slight dimensional differences the Corning tubes exhibit less crazing than the Falcon RUN TIMES TIME HR MIN rou wu g JA 12 Rotor Figure 8 Examples of Tubes with Minimum and Moderate Crazing The k factor of the rotor is a measure of the rotor s pelleting effi ciency Beckman Coulter has calculated the k factors for all of its rotors at maximum speed with full tubes using the following formula ants max min 102 Serr 1 o2 3600 where wis the angular velocity of the rotor in radians per second 0 105 x rpm Fmax is the maximum radius and rmin is the minimum radius After substitution gx 2 533 x 10 h In nax min 2 rpm 17 JA 12 Rotor 18 2 Based on sedimentation in water at 20 C In the JA 12 rotor the k factor is 1244 for full 50 mL tubes at maximum speed F
21. or containers that nominally hold less than 50 mL the k factors will be different Calculate the k factors for these containers using equation 2 Use the k factor in the following equation to estimate the run time t in hours required to pellet particles of known sedimentation coeffi cient s in Svedberg units S pak 3 S For example at 12 000 rpm E coli s 3600 S will pellet in 1244 t 3600 30 min 4 Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows 2 k 12 000 Pg t adj rated actual run speed 5 Run times can also be estimated from data established in prior experi ments using a different rotor if the k factor of the previous rotor is known For any two rotors a and b 2 6 where the k factors have been adjusted for the actual run speed used For more information on k factors see Use of k Factor for Estimating Run Times from Previously Established Run Conditions publication DS 719 RUN SPEEDS SPEED RPM RCF a1 eT Ll LL ot JA 12 Rotor The centrifugal force at a given radius in a rotor is a function of run speed Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields RCF When rota tional speed is selected so that identical samples are subjected to the same RCF in two different rotors the samples are subjected to the same force see Table 4 Do not select
22. otor Apply all appropriate safety and decontamination procedures to the centrifuge and accessories Never exceed the maximum rated speed of the rotor and labware in use Refer to the section on RUN SPEEDS Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use JA 12 Rotor JA 12 FIXED ANGLE ROTOR Axis of Rotation SPECIFICATIONS Maximum speed ecset erse chee cee erti enea one Sees 12 000 rpm Critical speed range 2 unuunu 400 to 1000 rpm Density rating at maximum speed 0 000 eee eee 1 2 g mL Maximum allowable imbalance of opposing loads 2 grams Relative Centrifugal Field at maximum speed at rmax 144 mm 0 0 ee ees 23 200 x g at ray 108 mm cee eee 17 400 x g at rmin 71 mm 1 eee eens 11 500 x g k factor at maximum speed 0 0 c cece eee eee eee 1244 Conditions requiring speed reduction see RUN SPEEDS Number of tube cavities 0 unuunu eee tee eee 12 Available bottles and tubes 00 cee eee eee eee see Table 3 Nominal tube dimensions 0000s eee eee 30 x 115 mm Nominal tube capacity 0 0 eee eee 50 mL Nominal rotor capacity 0 0 eee eee eee 600 mL Approximate acceleration time to maximum speed rotor fully loaded 1 0 00 0 0 3 1 2 min Approximate deceleration t
23. pins appear damaged contact Beckman Coulter Field Service 19 JA 12 Rotor Table 4 Relative Centrifugal Fields for the JA 12 Rotor Entries in this table are calculated from the formula RCF 1 12 r RPM 1000 and then rounded to three significant digits Relative Centrifugal Field x g Rotor Speed At max At fay At min rpm 144 mm 108 mm 71 mm 12 000 23 200 17 400 11 500 11 000 19 500 14 600 9 620 10 000 16 100 12 100 7 950 9 000 13 100 9 800 6 440 8 000 10 300 7 740 5 090 7 000 7 900 5 930 3 900 6 000 5 810 4 360 2 860 5 000 4 030 3 020 1 990 4 000 2 580 1 940 1 270 3 000 1 450 1 090 716 2 000 645 484 318 Relative Centrifugal Fields for the JA 12 Rotor 24 000 20 000 16 000 20 RCF x g 12 000 8 000 4 000 Speed rpm CLEANING Rotor Cleaning Kit 339558 i _ Small O ring Se Large O ring JA 12 Rotor e Regularly lubricate the metal threads in the rotor and on the tie down bolt with a thin even coat of Spinkote lubricant Failure to keep these threads lubricated can result in damaged threads e Regularly apply silicone vacuum grease to the O rings Replace O rings about twice a year or whenever worn or damaged Refer to Appendix A in Rotors and Tubes for chemical compatibili ties of rotor and accessory materials Your Beckman Coulter repre sentative prov
24. r Sales 1 800 742 2345 in the United States or your local Beckman Coulter office or see the High Perfor mance High Speed High Capacity Rotors Tubes amp Accessories catalog BR 8102 available at www beckmancoulter com for detailed information on ordering parts and supplies For your convenience a partial list is given below REPLACEMENT ROTOR PARTS JA 12 rotor assembly biosafe with dual locking lid 360992 JA 12 rotor assembly with single locking lid 360993 Dual locking lidie accesses Aes Res eee S 364846 Single locking lid na agian Aige eG GAS BASS AR a 360991 Tie down kit for use with dual locking lid 364922 Tie down kit for use with single locking lid 364919 Lid O ring ethylene propylene rubber 4 961697 Rotor body O ring ethylene propylene rubber 961696 Rotor removal tool 1 0 2 cee eee eee eee 360998 SUPPLIES TUDES ai 58 Sb Ab Ra see Rte beh etd ee eee oes see Table 3 Removal tooli c es los ha tis oe ea E ans 360998 Rotor Cleaning Kites cc 0c0 sbds cierre bees eae dee os 339558 Solution sss qt ese eat Rone hea ee Rode pede eens sod 339555 Silicone vacuum grease 1 OZ 0 ec eee ee eee 335148 Spinkote lubricant 2 0Z 0 0 eens 306812 25 J SERIES ROTOR WARRANTY Subject to the conditions specified below and the warranty clause of the Beckman Coulter Inc terms and conditions of sale in ef
25. tain the following information e rotor serial number e history of use approximate frequency of use e reason for the return e original purchase order number billing number and shipping number if possible e name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory and e name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that the parts are free from pathogens and or radioactivity Steriliza tion and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be enclosed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the out side of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label printed on the RGA form when mailing the rotor and or accessories Customers located outside the United States should contact their local Beckman Coulter office JA 12 Rotor SUPPLY LIST I gt NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1 800 742 2345 in the United States or by contacting your local Beckman Coulter office Contact Beckman Coulte
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