Home

NGC Installation Guide - Bio-Rad

1. Exporting Digital Data from the NGC Instrument You can convert digital NGC data to analog voltage via the SIM and output the voltage to an external recording device such as a light scattering detector The SIM converts and outputs NGC trace information as voltage through its digital to analog converter DAC ports By connecting a trigger wire to terminal 2 on the DIGITAL OUT connector the SIM can send a signal to the device triggering it to start measuring data Voltage from port 2 changes from High 5 V to Low 0 V when sample injection starts and returns to High when the injection stops When analog output is enabled through System Settings in ChromLab the external device can receive the converted trace information Connecting the SIM to the NGC Instrument To connect the SIM to an NGC instrument 1 Locate the peripheral cable that ships with the SIM 2 Insert one end of the peripheral cable into the peripheral port on the SIM peripheral Installation Guide 67 3 68 Setting Up NGC Chromatography Systems 3 Insert the other end of the cable into one of the peripheral connector p the side of the NGC instrument Soft power switch USB ports NGC Chromatography Systems and ChromLab Software orts on Peripheral connector ports Connecting Peripheral Equipment Connecting External Devices to the SIM WARNING These procedures require you to work with exposed wires Ensure t
2. C Connecting the C 96 Autosampler to NGC Systems Plumbing and Priming the C 96 Autosampler This section explains how to plumb the C 96 autosampler to the sample inject valve on the NGC instrument and to prime the autosampler after you plumb it Before you plumb and prime you must prepare the tubing Use 1 16 PEEK tubing to plumb the autosampler to the sample inject valve For information about plumbing the autosampler see the C 96 Autosampler for BioLogic DuoFlow Chromatography System Quick Start Guide or the C 96 Autosampler for BioLogic DuoFlow Chromatography System User Manual To prepare the tubing 1 2 Cut two segments of 1 16 PEEK tubing of adequate length To one end of each segment attach a 10 32 fitting This fitting attaches to the autosampler To the other end of each segment attach a 1 4 28 fitting This fitting attaches to the sample inject valve To connect the tubing from the autosampler to the sample inject valve 1 Ensure that both the NGC instrument and the C 96 autosampler are powered off Attach one 10 32 fitting to Port 1 and the other 10 32 fitting to Port 6 on the autosampler Attach the 1 4 28 fitting from Port 1 to the Loop E port on the sample inject valve Attach the 1 4 28 fitting from Port 6 to the Loop F port on the sample inject valve Insert one 1 4 28 plug to the syringe port and another 1 4 28 plug to the Waste port W1 on the sample inject valve Power on the NGC i
3. 155 B Troubleshooting ChromLab Software Installation 5 Right click the Local Area Connection icon and select Properties Lae Local Area Connection 7 malg Diable Status Diagnose Bridge Connections m Create Shortcut Delete 6 Inthe Local Area Connection Properties dialog box locate the Internal Protocol version 4 TCP IPv4 list item m Local Area Connection Properties X EP Intel R 82579LM Gigabit Network Connection This connection uses the following items o Client for Microsoft Networks E Trend Micro NDIS 6 0 Filter Driver JE QoS Packet Scheduler iE Fie and Printer Sharing for Microsoft Networks rs ee Link Layer Topology Discovery Responder e Transmission Control Protocol Intemet Protocol ine default wide area network protocol that provides communication across diverse interconnected networks K K K K K K K K ok ca 156 NGC Chromatography Systems and ChromLab Software Configuring the Network Adapter for Direct Connection Note If you do not see this item listed the computer is not set up for TCP IP Refer to the vendor for information about enabling TCP IP Select Internet Protocol Version 4 TCP IPv4 and click Properties The Internet Protocol version 4 TCP IPv4 Properties dialog box appears Make sure that Obtain an IP address automatically and Obtain DNS server a
4. NGC Chromatography Systems and ChromLab Software Installation Guide Version 3 3 NGC Chromatography Systems and ChromLab Software Installation Guide Version 3 3 Bio Rad Technical Support Department The Bio Rad Technical Support department in the U S is open Monday through Friday 5 00 AM to 5 00 PM Pacific time Go to www consult bio rad com for worldwide technical support Phone 1 800 424 6723 option 2 Web www consult bio rad com Email Support Bio Rad com U S Canada only For technical assistance outside the U S and Canada contact your local technical support office Notice No part of this publication may be reproduced or transmitted in any form or by any means electronic or mechanical including photocopy recording or any information storage or retrieval system without permission in writing from Bio Rad Bio Rad reserves the right to modify its products and services at any time This instrument guide is subject to change without notice Although prepared to ensure accuracy Bio Rad assumes no liability for errors or omissions or for any damage resulting from the application or use of this information Intel Core is a trademark of Intel Corporation PEEK is a trademark of Victrex plc Microsoft SQL Server and Windows are trademarks of Microsoft Corporation Copyright 2015 by Bio Rad Laboratories All rights reserved E Table of Contents Chapter INntroducuon ssas sassasrassar
5. Connect ChromLab To System 1 Launch ChromLab software on the connected computer 2 Connect to this system using the following Network Name NGC SN020007 IP Address 169 254 251 226 3 Click Continue after ChromLab is connected to this system System Information Continue Shut down Tip Write down the IP address You will need this number in the section Checking the Network Connection on page 160 158 NGC Chromatography Systems and ChromLab Software Troubleshooting Network Connection Issues IP Address Located in the System Information Dialog Box If the system is running you can find the IP address of the NGC instrument in the General tab in System Information To locate the IP address in the System Information dialog box ale On the touch screen expand the system s drop down list and select System Information Locate System IP Address in the General tab System Information General Devices Process Detector System Type System S N System Name System Computer ChromLab PC System SW Version NGC Quest 10 840BR000001 NGC LSG07002045 LSG07002045 Global Bio Rad Com Version 3 0 Copyright 2014 Bio Rad Laboratories Inc System Gateway System Network Name System MAC Address System Disk Space SBC BIOS version SBC BIOS build date Time Time Zone Up Time System IP Address 10 2 53 111 10 2 60 1
6. LSG07002045 2477034CB6DC 366771 MB Available A08 18 10 2011 2 10 2014 10 44 56 AM Pacific Standard Time GMT 8 22 min 38 sec Export Logs Close Write down the IP address You will need this number in the section Checking the Network Connection that follows Installation Guide 159 B 160 Troubleshooting ChromLab Software Installation Checking the Network Connection After you find the IP address of the instrument from the ChromLab computer verify that the instrument can be accessed through the network To check the network connection 1 2 On the ChromLab computer open a Command Prompt window At the prompt type gt ping xX X X X where x x x x is the IP address of the instrument Tip The IP address for an NGC instrument that is directly connected to the ChromLab computer begins with the number sequence 169 254 If the network connection is set up correctly you see results similar to the following image The ChromLab computer should be able to connect to the NGC instrument ia CAWindows system32 cmd exe l Microsoft Windows Version 6 1 76011 a opyright lt c 2689 Microsoft Corporation All rights reserved C Wsers NGC gt ping 169 254 251 226 Pinging 169 254 251 226 with 32 bytes of data bytes 32 time lt ims TTL 128 E 2 time lt ims TTL 128 eply from 169 254 251 226 byte 2 time lt ims TTL 128 Reply from 169 254 251 226
7. 1 Open the System Pump dialog box and select Priming on the Mode dropdown list Note When in Priming mode the sample inject valve switches the system pump flow to waste W2 After the process is completed the sample inject valve switches the system pump to manually load the column through the loop System Pump F10 Mode Priming Buffer Blending ports Select buffer blender port for priming Q1 OQ2 OQ3 O Q4 OAll Closed D Buffer Inlet ports Select inlet port for priming Inlet A Inlet B 8 Bypass 8 Bypass Duration J5 Min Flow Rate 0 002 20 20 000 7 ml min D Start m To purge an individual line of air bubbles proceed to step 2 on page 139 To purge all lines of air bubbles sequentially proceed to step 3 on page 139 138 NGC Chromatography Systems and ChromLab Software Priming and Purging the System To purge an individual line of air bubbles and fill it with buffer a Select the port to open b Optional Change the default flow rate The default flow rate is 20 ml min c Click Start d Repeat steps 2a c for any other port to purge To purge all lines of air bubbles and fill them with buffer a Select All b Optional Change the default flow rate The default flow rate is set to 20 ml min c Click Start The system pumps and the buffer blending valve start operating at the specified flow rate exchanging the solution in the inlet lines and expelling any tr
8. Fluidic Scheme NGC Discover cal o 0 0 B gt CE We o PreCol 148 psi ACol100 psi A1 215 nm Standby A2 255 nm Standby 23 280 nm Standby M4 495 nm Standby 26 8mS em Rack Tube 0 Volume 0 0 ml Tubes Remaining 0 Delay Volume Off e pH 7 00 Installation Guide 91 4 Installing ChromLab Software Setting Delay Volume 92 The delay volume is the volume of fluid in the path between the UV or UV Vis detector and the fraction collector drophead The system is typically plumbed with a fixed length of tubing and a defined set of detectors such as the conductivity monitor and pH detector between the UV or UV Vis detector and the fraction collector The length of tubing and the volume of the flow cells between the UV or UV Vis detector and the drophead define the delay volume Setting the delay volume causes the fraction collector to wait for the sample to travel from the UV or UV Vis detector to the drophead before it starts to collect sample fractions In order to accurately predict which fraction collector tube or outlet container contains the peak of interest you have to account for the time it takes for the volume of fluid to travel from the UV or UV Vis detector to the container Because the tubing length and flow cells between the detector and the fraction collector drophead do not change often you can set the
9. Insert a 30 ml syringe into the priming port on system pump A 7 Turn the priming port valve counter clockwise one full turn to open the seal 8 Slowly withdraw the plunger to withdraw liquid from the port until air no longer enters the syringe approximately 10 ml and the tubing is filled with liquid 9 Turn the priming port valve clockwise to close the port 10 Remove the syringe from the priming port and discard the liquid into waste 11 Repeat steps 6 10 for ports Q3 Q1 12 To prime system pump B select port Q3 and perform steps 6 10 128 NGC Chromatography Systems and ChromLab Software Priming and Purging the System Purging the NGC Scout System To purge the buffer blending valve 1 Open the System Pump dialog box and select Priming in the Mode dropdown list System Pump F10 Mode Priming Select buffer blender port for priming Q1 OQ2 OG O Q4 OAll Closed Duration 1 Flow Rate 0 002 20 20 000 5 ml min D Start m To purge an individual line of air bubbles proceed to step 2 on page 129 To purge all lines of air bubbles sequentially proceed to step 3 on page 130 2 To manually purge an individual line of air bubbles and fill it with buffer a Selecta port to open b Optional Change the default flow rate The default flow rate is set to 20 ml min c Click Start d Repeat steps 2a c for any other port to purge Installation Guide 129 5 Plumbing and Prim
10. System Name 0 2c e eee tee eee 86 setting Delay VOIUMG ssc tee banda eee eh ea OR ea ee ae ele ee pee 92 Setting Up Remote Access Connection 0 00 eee ee 95 iv NGC Chromatography Systems and ChromLab Software Table of Contents Chapter 5 Plumbing and Priming the NGC Chromatography Systems 2000 cece eee ees 97 Preparing the Buffer and Waste Bottles 0 0 0 0 c eee eee eee 98 Buffer Bottles 2 in6 ache oSaas Cogeco ee Lek ANENA Eee ene ee Gee 98 Waste Boles 2 ee cs EPOE bi ear dads PER es Pee ene ee Mi ey Ee 98 Plumbing NGC Systems s he0ssdes etka ceed debe ede ae een cog da Bees 99 General Guidelines for Tubing Sizes 6 ee 99 Plumbing All NGC Systems 0 2 00 eee ee 100 Plumbing the NGC Quest System 0 0 ce ee 106 Plumbing the NGC Scout System 0 0 cee eee 109 Plumbing the NGC Discover System 0c eee eee 113 Plumbing the NGC Discover Pro System 00 0c eee eee 118 Priming and Purging the System so 2 0 00 0 e eee ee 123 Priming and Purging the NGC Quest System 000 e ee eee 124 Priming and Purging the NGC Scout System 00 0c eee eee 127 Priming and Purging the NGC Discover System 200 eae 131 Calibrating the Instrument 2 20 0 eee 144 Calibrating PH 23 cceenigees pease ieee ne nian bane tee ne ne ee be 144 Calibrating the Pump Flow Rate 0 00 ee ee 145 Calibrating Pressure Sensor Zer
11. and Priming the NGC Chromatography Systems With the exception of the inlet tubing waste lines and column the NGC systems ship preassembled and preplumbed with the modules necessary to perform gradient separations The systems require minimal postinstallation setup to prepare them to run simple gradient separations This chapter explains how to plumb and prime your system after you have installed it and the ChromLab software Installation Guide 97 5 Plumbing and Priming the NGC Chromatography Systems Preparing the Buffer and Waste Bottles Before you plumb and prime the NGC instrument prepare buffer and waste bottles Buffer Bottles Place buffer bottles on the bench next to the instrument or in the buffer tray on top of the instrument The tray can accommodate up to six 1 L bottles A oo Waste Bottles Place the waste bottles on top of the bench or on the floor below the instrument Ensure that the waste containers are large enough to hold all buffer that will flow into them Empty the waste bottles regularly Important Do not place waste bottles on top of the instrument 98 NGC Chromatography Systems and ChromLab Software Plumbing NGC Systems Plumbing NGC Systems Several plumbing tasks are required for all instrument configurations Other tasks are specific to the configuration This section explains how to plumb each instrument configuration General Guidelines for Tubing Sizes The NGC instrument
12. are not installed the wizard installs them before installing ChromLab The wizard also installs and configures the ChromLab database instance After you install ChromLab you will configure the NGC system to use the software You might also set the delay volume for your system This chapter explains these tasks Installation Guide 81 4 Installing ChromLab Software Installing ChromLab Software To install ChromLab software 1 Insert the ChromLab Software CD in your CD ROM drive The ChromLab installer automatically launches Welcome to the Install Wizard for ChromLab Software ChromLab Software The InstallShield R Wizard will allow you to modify repair or remove ChromLab Software To continue click Next 2 On the Welcome screen click Next 3 Accept the license agreement and click Next 4 On the Ready to Install the Program screen click Install Ready to Modify the Program The wizard is ready to begin installation Click Install to begin the installation If you want to review or change any of your installation settings click Back Click Cancel to exit the wizard InstallShield 82 NGC Chromatography Systems and ChromLab Software Installing ChromLab Software The wizard installs ChromLab Installing ChromLab Software The program features you selected are being installed Pr Please wait while the Install Wizard installs ChromLab Software T
13. attach the touch screen on the left side of the NGC instrument If you plan to attach the touch screen on the right side of the instrument you must reposition the touch screen s pivot arm assembly See Repositioning the Touch Screen Pivot Arm Assembly on page 31 for more information To attach the touch screen when the NGC system is on a lab bench 1 Determine the side of the NGC instrument right or left on which to attach the touch screen 2 Remove the side mount cover from the side of the instrument 24 NGC Chromatography Systems and ChromLab Software Attaching the Touch Screen Cable trough cover Side mount cover Note The top section in this image is a third tier The NGC Discover system is a 3 tier system The touch screen is attached at the top of the second tier If you have an NGC Quest or an NGC Scout system you will not have a third tier Locate the side mount cover at the top of the second tier Using a Phillips screwdriver remove the screw that secures the cable trough cover to the back of the instrument Note Store these components in a safe place for future use Installation Guide 25 3 Setting Up NGC Chromatography Systems 4 Using two 20 x 34 hex bolts and the hex key labeled pumphead provided in the NGC accessories kit attach the touch screen mount to the side of the NGC instrument 5 26 NGC Chromatography Sys
14. bytes 32 time lt ims TIL 128 ics for 169 254 251 226 Sent 4 Received 4 Lost loss Approximate round trip times in milli second Minimum ms Maximum ms Average Gms Users NGC gt _ NGC Chromatography Systems and ChromLab Software Troubleshooting Network Connection Issues m If the network is not set up correctly you see results similar to this image Se EA C Windows system32 cmd exe a Microsoft Windows Version 6 Copyright lt c 2009 Microsoft Corporation All rights reserved iC Users NGO gt ping 169 254 251 226 Pinging 169 254 251 226 with 32 bytes of data R Destination host unreachable Destination host unreachable Destination host unreachable Reply from 169 254 117 241 Destination host unreachable ing statistics for 169 254 251 226 Packets Sent 4 Received 4 Lost loss SERSIES3 3S el ep a The ChromLab computer cannot connect to the instrument Check the setup of the network adapter in the section Configuring the Network Adapter for Direct Connection on page 154 Make sure TCP IPv4 is installed and set up for DHCP Installation Guide 161 B Troubleshooting ChromLab Software Installation ChromLab Software Firewall Settings If your computer has Windows Firewall turned on check your firewall settings and ensure that the following programs and services required for ChromLab are allowed to communicate with your compu
15. c Insert the wires into the open ports in this order and tighten the screws m Insert the red wire into the first port m Insert the black wire into the second port m Insert the brown wire into the last ground port Note Take care not to overtighten or fray the wires Attach the DIGITAL OUT connector to the 9 pin D connector cable Tip The 9 pin D connector cable has two wires red and black a From left to right loosen the first and last screws on the connector b Insert the wires into the open ports in this order and tighten the screws taking care not to overtighten or fray the wires Insert the red wire into the last port Insert the black wire into the first ground port 166 NGC Chromatography Systems and ChromLab Software Using the C 96 Autosampler with NGC Systems 8 On the back of the autosampler a Insert the 15 pin cable into the left P1 RELAYS OUTPUTS connector port and tighten the thumbscrews Insert the 9 pin cable into the right P2 INPUTS connector port and tighten the thumbscrews 15 pin connector 9 pin connector P1 P2 RELAYS OUTPUTS INTPUTS k 9 Onthe back of the SIM a b Insert the P1 RELAYS OUTPUTS cable into the DIGITAL IN port Insert the P2 INPUTS cable into the DIGITAL OUT port 9 pin connector 15 pin connector DAC DIGITALOUT DIGITALIN SM1 SM2 B 0 10V A ies faa accra 1234GND GND GND Installation Guide 167
16. cap and shake downward to move the bubbles into the stem Carefully remove the plastic cap from the pH probe Inspect the pH probe and ensure that the O ring is in place on the body Insert the pH probe into the top of the pH flow cell Slide the collar over the pH probe onto the pH valve flow cell and carefully hand tighten the collar until it is snug Caution Do not overtighten the collar Overtightening will break the glass pH probe Connect the pH probe cable to the DNS connector on the pH valve module Tip If a small amount of liquid drips from the flow cell during installation wipe it off with a paper towel 42 NGC Chromatography Systems and ChromLab Software Installing the pH Probe in the pH Module 10 Start the NGC instrument The following figure shows the pH probe installed in the flow cell DNS connector Nut on DNS cable PH collar 11 Calibrate the pH monitor Note See the NGC Chromatography Systems and ChromLab Software User Guide for information about calibrating the pH monitor Installation Guide 43 3 Setting Up NGC Chromatography Systems Attaching an Expansion Tier to the NGC Instrument A 44 WARNING Disconnect power to the NGC instrument before attaching an expansion tier Do not attempt to service any component on the NGC instrument unless noted in this manual Contact Bio Rad for service requests To attach the connector cable you will need access to the b
17. components For information about installing and setting up an external device see its installation documentation Controlling Programmed Functions via Transistor to Transistor Logic You can control a function of an external device or receive function information from a device via the SIM Using the SIM s transistor to transistor logic TTL the NGC instrument can mark when a function occurred on an external device through the SIM s DIGITAL IN port or send commands to an external device through its DIGITAL OUT port For example you can connect a C 96 autosampler to the SIM enabling NGC systems to provide automated accurate and reproducible sample injections Connecting the autosampler to the SIM s DIGITAL OUT and DIGITAL IN ports enables the NGC instrument to inform the autosampler when to begin its injection cycle and to mark when the autosampler injects a sample 66 NGC Chromatography Systems and ChromLab Software Connecting Peripheral Equipment Importing Analog Signals to the NGC Instrument You can import analog voltage signal from up to two external devices and convert it to digital NGC data via the SIM The SIM imports and converts the signal to the appropriate trace units through its SIM 1 and SIM 2 ports When data import is enabled through System Settings in ChromLab the details appear in the chromatogram s legend the trace appears on the chromatogram and the data can be analyzed in the Evaluation window
18. connector port INSTRUMENT BUS port omm m i i ma omo e lI DISPLAY DIVERTER ee vo I VALVE INSTRUMENT BUS ANALOG IN 1 VOLT MAX REC i E 1 Q 4 Insert the other end of the cable into the INSTR BUS connector on the back of the communication adapter INSTR BUS i o W ig Tip The RX and DX diodes are communication indicators You see the lights flash as data transfer between the fraction collector and the NGC instrument The COMM port is not used to connect to the fraction collector 5 Locate the peripheral cable that ships with the communication adapter Installation Guide 57 3 Setting Up NGC Chromatography Systems 6 Insert one end of the peripheral cable into the peripheral port on the communication adapter peripheral 7 Insert the other end of the cable into one of the peripheral ports on the NGC instrument Soft power switch Peripheral connector ports USB ports 58 NGC Chromatography Systems and ChromLab Software Connecting Peripheral Equipment 8 Power on the instruments a Power on the BioFrac fraction collector first b Press the soft power switch on the NGC instrument to power it on Connecting Column Clamps to the NGC Instrument The NGC instrument has indented metalli
19. entering into the module s electronic circuitry Caution All air sensors connected to the air sensor module must be filled with liquid and devoid of air A connected air sensor that is not plumbed and primed can trigger an air bubble sensing event which causes the pumps to stop and A the blue air sensor LEDs to turn on 62 NGC Chromatography Systems and ChromLab Software Connecting Peripheral Equipment Air Sensor Tubing Length and Bubble Size The response of the air sensor module is highly dependent on the bubble size and flow rate and requires a minimum tubing length between the sensor outlet and the pump inlet Table 6 lists recommended tubing lengths for various flow rates and bubble sizes Table 6 Recommended air sensor tubing lengths Flow Rate Bubble Size 1 8 ID FEP Tubing Length air sensor to pump inlet 1 ml min 15 25 ul 26 5 ml min 50 100 pl 26 10 ml min 200 ul 26 100 ml min 1 05 1 4 ml 242 Installation Guide 63 3 Setting Up NGC Chromatography Systems Attaching and Activating Air Sensors This section explains how to attach the air sensor module and the air sensors It also explains how to activate the air sensors after they are attached Note Bio Rad recommends that all buffers be filtered and degassed prior to use To attach the air sensor module and air sensors 1 Ensure that the NGC instrument is powered off 2 Attach the air sensor module to one of the indented metallic strips on the si
20. inlet tubing is color coded to identify the different buffer solutions The color scheme for the tubing and ports is defined as follows Tubing color Port Solution Green Q4 High salt buffer Yellow Q3 Water Blue Q2 Buffer base Red Q1 Buffer acid To plumb the pH valve 1 Connect the pump head wash priming tubing to the Cal In port on the pH valve 2 Connect waste tubing to the Cal Out port on the pH valve Installation Guide 111 5 Plumbing and Priming the NGC Chromatography Systems Calibration Outlet to waste port fraction collector Calibration Inlet from injection port conductivity flow cell 3 Immerse the open end of the waste tubing in the waste container 4 Connect Tube 2 from the 20 psi backpressure regulator that you connected in step 2 on page 105 to the inlet port In on the pH valve 5 Connect Tube 4 to the outlet port Out on the pH valve 6 Connect the other end of Tube 4 to the Common port on the BioFrac fraction collector diverter valve Tip To clean the pH valve immerse the outlet lines into a waste container and the inlet lines into cleaning solution Perform the system wash step in ChromLab as explained in the section System CIP Clean in Place Storage Parameters in the NGC Chromatography Systems and ChromLab Software User Guide 112 NGC Chromatography Systems and ChromLab Software Plumbing NGC Systems Plumbing the NGC Discover System Table 11 lists
21. ml min Mode Isocratic Gradient Duration 2 min Pressure Limits Lower 0 pa psi Upper 3650 psi Y E Control Flow to avoid overpressure Gag Sra Installation Guide 171 C Connecting the C 96 Autosampler to NGC Systems Setting Up the C 96 Autosampler for Use in ChromLab Methods This section briefly explains how to set up the autosampler for use in a ChromLab method For information about creating methods in ChromLab see the NGC Chromatography Systems and ChromLab Software User Guide To set up a method that uses the autosampler 1 In ChromLab create a method that includes the autosampler in the fluidic scheme The Sample Application phase automatically displays Inject Using Autosampler 2 Inthe Sample Application phase increase the injection volume to include the total volume of the installed autosampler loop and the tubing needed to connect Port 6 on the autosampler to the Loop F port on the sample inject valve Tip Typically a sample loading volume of 2 3 ml should be sufficient to completely load the entire sample in the autosampler loop onto the column 3 Use the front panel on the C 96 autosampler to create a method See the C 96 Autosampler for BioLogic DuoFlow Chromatography System Quick Start Guide or the C 96 Autosampler for BioLogic DuoFlow Chromatography System User Manual for more information 4 Start the autosampler method and place the autosampler in REMOTE m
22. system pump to manually load the column through the loop Select Q4 as the port to open and prime NGC Chromatography Systems and ChromLab Software 10 11 12 13 Priming and Purging the System System Pump F10 Made Priming Buffer Blending ports Select buffer blender port for priming Q1 O Q2 O Q3 O Q4 OA Closed Buffer Inlet ports Select inlet port for priming Inlet A Inlet B 8 Bypass s a Duration sl Min Flow Rate 0 002 20 20 000 5 ml min DP Start Insert a 30 ml syringe into the priming port on system pump A Turn the priming port valve counter clockwise one full turn to open the seal Slowly withdraw the plunger to withdraw liquid from the port until air no longer enters the syringe approximately 10 ml and all tubing is filled with liquid Turn the priming port valve clockwise to close the port Remove the syringe from the priming port and discard the liquid into waste Repeat steps 7 11 for ports Q3 Q1 To prime system pump B select port Q3 and perform steps 7 11 Installation Guide 133 5 Plumbing and Priming the NGC Chromatography Systems To prime the buffer inlet valves 1 Immerse the tubing for the inlet ports in their respective buffer containers 2 Onthe NGC touch screen open the System Pump dialog box 3 Inthe System Pump dialog box select the first inlet port to prime for example Buffer A 1 System Pump F10 Mode Pr
23. the tasks for plumbing the NGC Discover system The tasks are explained in detail in the sections that follow Note Ensure that you complete the plumbing tasks in the section Plumbing Alll NGC Systems on page 100 before continuing Table 11 Plumbing tasks for the NGC Discover system Tasks 11 Inlet tubing to the left pump head wash port on the sample pump Pump head wash priming line to the right pump head wash port on the sample pump 1 4 28 union to the pump head wash priming line on the sample pump Inlet tubing to the other end of the 1 4 28 union on the sample pump Inlet tubing to ports Q1 Q4 on the buffer blending valve Inlet tubing to the remaining ports on the buffer inlet valves Inlet tubing to the inlet manifold on the sample pump Waste tubing to the Cal Out port on the pH valve Priming line to the calibration inlet port Cal In on the pH valve Tube 2 from the 20 psi backpressure regulator to the inlet of the pH valve Tube 4 from the 20 psi backpressure regulator to the Common inlet port on the BioFrac fraction collector diverter valve The figure on page 114 shows the Point to Plumb connections for the NGC Discover system Installation Guide 113 5 Plumbing and Priming the NGC Chromatography Systems NGC Discover Point to Plumb Connections Q3 WATER r A g 4 TO WASTE INLET N N Oe O f Zap as D t b a 2 a SAMPLE LOO
24. 0 India 91 124 4029300 Israel 03 963 6050 Italy 39 02 216091 Japan 81 3 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666 New Zealand 64 9 415 2280 Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04 Singapore 65 6415 3188 South Africa 27 0 861 246 723 Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland 026 674 55 05 Taiwan 886 2 2578 7189 Thailand 1800 88 22 88 United Kingdom 020 8328 2000 15 0550 0315 Sig 1214
25. D drive USB 2 0 high speed ports 2 minimum Mouse Keyboard 2 gigabit ethernet port 1 minimum 1 200 x 900 1 280 x 1 024 recommended True color mode 24 bits or higher 20 or higher NGC Chromatography Systems and ChromLab Software Preparing the ChromLab Computer Preparing the ChromLab Computer To ensure the ChromLab computer operates the NGC system smoothly and accurately displays ChromLab software you might need to Turn off sleep hibernate mode Turn off automatic updates Set the display fonts To turn off sleep hibernate mode 1 Onthe ChromLab computer select Control Panel gt Power Options and click Change plan settings for the selected power plan 2 On the Change settings for the plan page click Change advanced power settings 3 On the Advanced settings tab expand the Sleep option then expand Sleep after and do one of the following m If your ChromLab computer is a laptop click On battery or Plugged in or both and select Never from the dropdown list m If your ChromLab computer is a desktop computer click Setting and select Never from the dropdown list 4 On the Advanced settings tab expand Hibernate after and then do one of the following m If your ChromLab computer is a laptop click On battery or Plugged in or both and select Never from the dropdown list m If your ChromLab computer is a desktop computer click Setting and select Never from the dropdown list 5 Click OK to s
26. GC Discover Pro series 34 in 86 4 cm Note These dimensions are for the NGC instrument only They do not include the touch screen or accessories such as columns air detectors and buffer tray NGC instrument weight Instrument top with buffer tray NGC Quest 90 Ib 40 9 kg NGC Quest Plus 97 Ib 44 0 kg NGC Scout 94 Ib 42 7 kg NGC Scout Plus 100 Ib 45 4 kg NGC Discover 141 Ib 64 kg NGC Discover Pro 171 Ib 77 6 kg Height 3 in 7 6 cm Weight 8 Ib 3 6 kg Installation Guide 15 2 Preparing Your Site Table 2 Specifications for the NGC instrument continued Item Specification Touch screen dimensions Height 10 in 25 4 cm Length m Arm contracted 13 in 33 0 cm m Arm extended 15 in 38 1 cm Width m Arm contracted 1 in 2 5 cm m Arm extended 4 in 10 2 cm Touch screen weight with arm 4 Ib 1 8 kg Environment Table 3 Supported environmental specifications Parameter Temperature Range Humidity Range Operating conditions 4 to 40 C 0 95 RH 35 to 104 F noncondensing Storage conditions 4 to 40 C 0 95 RH 35 to 104 F noncondensing Shipping conditions 40 to 65 C 0 95 RH 40 to 149 F noncondensing 16 NGC Chromatography Systems and ChromLab Software Power Requirements Power Requirements The power supply to the NGC system must be stable and within specifications at all times to ensure optimal operation The power cable connected to the power inlet port must be r
27. NGC Scout system is a manual procedure and involves priming the buffer blending valve starting from the Q4 port to Q1 Purging the NGC Scout system is an automated procedure and involves removing air from one or all buffer lines as well as the system pumps Both procedures require access to ChromLab Tip You can perform the steps on the NGC touch screen Priming the NGC Scout System Note This section assumes that you have not introduced buffer into the lines If you have buffer in the lines and are purging the lines of small air bubbles proceed to Purging the NGC Scout System on page 129 To prime the buffer blending valve 1 Immerse the inlet tubing for ports Q4 Q1 in their respective buffer containers See To plumb the buffer blending valve on page 111 for more information 2 Set the fluidic scheme to either NGC Scout or NGC Scout Plus 3 Inthe fluidic scheme touch or click the System Pump module to open its dialog box a oT C 4 Inthe System Pump dialog box select Priming in the Mode dropdown list By default all ports are closed The Closed radio button is selected Installation Guide 127 5 Plumbing and Priming the NGC Chromatography Systems 5 Select Q4 as the port to open and prime System Pump F10 2 a8 Mode Priming X Select buffer blender port for priming Ql O Q2 O Q3 O Q4 O All Closed Duration 1 Flow Rate 0 002 20 20 000 D Start 6
28. Orange 0 02 0 5 mm Green 0 03 75 mm Clear 0 062 1 6 mm If your system includes the BioFrac fraction collector select the checkbox Include BioFrac 12 ul and specify the tubing length between the UV outlet port and the fraction collector drophead If your system includes an outlet valve select the checkbox Include Outlet valve 50 ul and specify the tubing length between the UV outlet port and outlet valve Installation Guide 93 4 94 Installing ChromLab Software T If your system includes both the BioFrac fraction collector and an outlet valve select both checkboxes In the Include Outlet valve 50 ul section specify the tubing length between the UV outlet port and outlet valve as well as the tubing length between the outlet valve and the fraction collector drophead Select or clear the next four checkboxes depending on whether or not these modules are present on your system Note If a pH detector module is present on your system and you select the checkbox to include its cell volume determine whether the detector is set to Inline or Bypass mode and select the appropriate radio button If one or more of the options is not applicable or if you change the length of the tubing enter the appropriate values in the Additional volume box The system calculates the delay volume based on your specifications and displays the results in pl at the bottom of the dialog box 10 Click OK NGC Chromatography System
29. P eee Q J 3 So N D o J TO WASTE A gt L hd p td Tubing Key Green PEEK F100 pumps Orange PEEK F10 pumps Clear FEP tubing Clear waste tubing O Point to Plumb connections Pre installed 114 NGC Chromatography Systems and ChromLab Software Plumbing NGC Systems To plumb the buffer blending valve gt Connect the inlet tubing to ports Q1 Q4 on the buffer blending valve Note The buffer inlet tubing is color coded to identify the different buffer solutions The color scheme for the tubing and ports is defined as follows Tubing color Port Solution Green Q4 High salt buffer Yellow Q3 Water Blue Q2 Buffer base Red Q1 Buffer acid Installation Guide 115 5 116 Plumbing and Priming the NGC Chromatography Systems To plumb the buffer inlet valves ae Connect tubing from Outlet A on the buffer blending valve to port 8 BB on buffer inlet valve A Connect tubing from Outlet B on the buffer blending valve to port 8 BB on inlet valve B Connect tubing from the Out port on inlet valve A to the inlet manifold of system pump A Connect tubing from the Out port on inlet valve B to the inlet manifold of system pump B Optional Connect inlet tubing to ports 2 7 on both buffer inlet valves A and B To plumb the sample pump 1 2 Connect inlet tubing to the sample pump inlet manifold Immerse the other end of the in
30. P Address You will need one of these values when you start ChromLab on the PC Important Do not click Continue at this time Connect ChromLab To System 1 Launch ChromLab software on the connected computer 2 Connect to this system using the following Network Name NGC SN020007 IP Address 169 254 251 226 3 Click Continue after ChromLab is connected to this system System Information Continue Shut down Note The following section is performed on the ChromLab computer Installation Guide 87 4 Installing ChromLab Software On the ChromLab computer 1 Start ChromLab The System Connection dialog box appears ChromLab detects the NGC systems available on the same sub network or those systems that are directly connected to the computer The system name network name and IP address of each available system appear in the table WF System Connection Select detected system System Name Network Name NGC Rm342 NGC SN020007 NGC Rm201 NGC SN020001 IP Address 10 2 55 225 169 254 157 2 88 Enter network name or IP address Connect Close Note If your system does not appear in the table click Detect to refresh the list ChromLab searches the network for available NGC systems and refreshes the list In the System Connection dialog box do one of the following m Select a name in the list of detected systems and click Connect Select the appropriate
31. Scout Specification Length 32 in 81 3 cm Width 27 in 68 6 cm Height 33 in 83 8 cm Weight 161 Ib 73 1 kg Length 32 in 8138 cm Width 27 in 68 6 cm Height 33 in 83 8 cm Weight 167 Ib 75 8 kg Length 32 in 81 3 cm Width 27 in 68 6 cm Height 33 in 83 8 cm Weight 166 Ib 75 4 kg Installation Guide 13 2 Preparing Your Site Table 1 Specifications for the NGC delivery crate continued Item Specification NGC Scout Plus Length 32 in 81 3 cm Width 27 in 68 6 cm Height 33 in 83 8 cm Weight 172 Ib 78 1 kg NGC Discover Length 32 in 81 3 cm Width 27 in 68 6 cm Height 41 in 104 1 cm Weight 216 Ib 98 1 kg Individual tier for Length 27 in 68 6 cm example the fourth tier on Width 27 in 68 6 cm the NGC Discovery Pro Height 14 in 35 6 cm system Weight 40 Ib 18 2 kg Note The fourth tier the upper tier on the NGC Discovery Pro system is packaged separately 14 NGC Chromatography Systems and ChromLab Software Room Requirements Bench Space Room Requirements Ensure that the bench or rack on which you install the NGC system is designed to support its weight Table 2 Specifications for the NGC instrument Item NGC instrument dimensions Specification Length 24 in 61 0 cm Width 19 in 48 3 cm Height measured from lab bench m NGC Quest and NGC Scout series 19 in 48 3 cm NGC Discover series 26 5 in 67 3 cm N
32. Sensor Zero Point Use this procedure to zero the pressure on the following modules Sample pump System pump Column switching valve To zero the pressure 1 Ensure the modules are not plumbed and are at ambient pressure On the NGC touch screen select Calibrate on the ChromLab dropdown menu Select the pump or valve on the dropdown list Follow the steps in the Calibration dialog box oe 1 lM Verify that Status at the bottom of the dialog box is Calibrated Installation Guide 145 5 146 Plumbing and Priming the NGC Chromatography Systems Calibrating the Conductivity Monitor Flow conductivity standard from any commercial supplier through the conductivity flow cell until a stable reading is obtained To calibrate the conductivity monitor 1 2 3 4 On the NGC touch screen select Calibrate on the ChromLab dropdown menu Select Conductivity Monitor on the dropdown list Follow the steps in the Conductivity Monitor dialog box Verify that Status at the bottom of the dialog box is Calibrated NGC Chromatography Systems and ChromLab Software A Upgrading ChromLab Software and NGC Systems This appendix explains how to upgrade ChromLab software and your NGC system from a previous version to the current version Caution Before beginning the upgrade process ensure that you have saved all manual runs and that no methods are running Upgrading ChromLab Software Note Upgrading ChromL
33. Systems Updating the NGC system is a two step process Updating ChromLab software on the instrument Updating the firmware on the instrument Between the software and firmware update the NGC instrument automatically reboots The instrument reboots following a successful firmware upgrade as well To update the NGC system 1 On the ChromLab computer start ChromLab ChromLab automatically connects to the instrument it was last connected to before the software update 2 A dialog box appears prompting you to update the software on the system Click Update System 148 NGC Chromatography Systems and ChromLab Software Updating NGC Systems ChromLab Cannot connect to system due to version mismatch ChromLab version 3 0 0 03 System version 2 0 0 54 Make sure the pumps are stopped Update System Important The update process can up to 20 minutes A progress dialog box appears on the NGC touch screen with information about the time remaining for the update Do not shut down the instrument during the update process After the update process completes the instrument automatically reboots The system checks the firmware versions on the modules If the firmware is not current the Firmware Version Warning dialog box appears Click Update Now The modules listed below do not have the expected version of firmware Update firmware to the latest version to ensure that the system functions properly Tim
34. ab software from version 1 0 to the current version does not retain customized system settings If you customized the system settings in version 1 0 you will need to reapply your changes after the upgrade Upgrading ChromLab Software to the Current Version To upgrade ChromLab software to the current version T Insert the ChromLab Software CD into your CD ROM drive The ChromLab installer automatically launches If the ChromLab installer does not automatically start a In Windows Explorer navigate to and open the ChromLab Software CD drive b Double click ChromLab_Setup exe to start the ChromLab installer The installer automatically detects that an earlier version of ChromLab is installed and removes it before installing the current version Note Upgrading ChromLab and the database can take several minutes When the upgrade process is complete click Finish to exit the wizard Installation Guide 147 A Upgrading ChromLab Software and NGC Systems Resetting Customized System Settings Tip Perform this task only if you are upgrading from ChromLab 1 0 to the current version If you are upgrading from a later version for example version 2 0 you can skip this section To reset customized system settings 1 Inthe System Control window select File gt System Settings to open the System Settings dialog box 2 Choose the appropriate tab and re enter the changes 3 Click OK to save the changes Updating NGC
35. able 10 lists the tasks for plumbing the NGC Scout system The tasks are explained in detail in the sections that follow Note Ensure that you complete the plumbing tasks in the section Plumbing Alll NGC Systems on page 100 before continuing Table 10 Plumbing tasks for the NGC Scout system NGC Scout 1 Inlet tubing to ports Q1 Q4 on the buffer blending valve 2 Waste tubing to the calibration outlet port Cal Out on the pH valve 3 Pump head wash priming line to the calibration inlet port Cal In on pH valve 4 Tube 2 from the 20 psi backpressure regulator to the inlet port on the pH valve 5 Tube 4 from the outlet port on the pH valve to the Common inlet port on the BioFrac fraction collector diverter valve The figure on page 110 shows the Point to Plumb connections for the NGC Scout system Installation Guide 109 5 Plumbing and Priming the NGC Chromatography Systems NGC Scout Point to Plumb Connections Q4 Q3 Q2 Q1 ACID BASE WATER SALT TO WASTE Tubing Key Green PEEK F100 pumps Orange PEEK F10 pumps Clear FEP tubing Clear waste tubing O Point to Plumb connections Pre installed Q OUTLET Q OUTLET 110 NGC Chromatography Systems and ChromLab Software Plumbing NGC Systems To plumb the buffer blending valve gt Connect the inlet tubing to ports Q1 Q4 on the buffer blending valve Note The buffer
36. ack of the NGC instrument If necessary pull the NGC instrument away from the wall and rotate the instrument to gain access Important If you are adding a fourth expansion tier you must attach it to an already attached third tier The third and fourth tiers are not interchangeable Ensure that the third tier is installed on the NGC instrument before attaching the fourth tier To attach an expansion tier to the NGC instrument 1 On the touch screen select Shut Down on the dropdown menu to exit ChromLab and shut down the NGC instrument 2 Remove all tubing lines to sample and buffer bottles and remove any bottles from the buffer tray 3 Lift the top off the NGC instrument and place it on the lab bench NGC Chromatography Systems and ChromLab Software Attaching an Expansion Tier to the NGC Instrument Tip The buffer tray can remain inside the top Locate the package that contains the expansion tier and 52 1 32 m connector cable Important The cables that ship with third and fourth tiers are not interchangeable You must use the cable that ships with the expansion tier Inserting a third tier cable into the fourth tier connector ports will damage the cable and the ports The catalog number for the 25 63 5 cm second to third tier connector cable is 100 24878 The catalog number for the 52 1 32 m third to fourth tier connector cable is 100 24892 Carefully remove the tier from its packaging material In
37. ad wash priming tubing to the right rear port Installation Guide 103 5 Plumbing and Priming the NGC Chromatography Systems Plumbing the Sample Inject Valve To plumb the sample inject valve 1 Connect waste tubing to waste ports Waste 1 and Waste 2 on the sample inject valve 2 Connect an injection port to the center of the sample inject valve Injection port e Sample Pump 3 Connect the sample loop to ports Loop E and Loop F on the inject valve 104 NGC Chromatography Systems and ChromLab Software Plumbing NGC Systems Attaching the Backpressure Regulator If your column requires a high backpressure install the backpressure regulator To attach the backpressure regulator 1 Connect Tube 2 to the top of the conductivity monitor 2 Connect the other end of Tube 2 to a 20 psi backpressure regulator Note The backpressure regulator has a raised arrow on its side Ensure that the arrow on the regulator points in the direction of the flow Installation Guide 105 5 Plumbing and Priming the NGC Chromatography Systems Plumbing the NGC Quest System Table 9 lists the tasks for plumbing the NGC Quest system The tasks are explained in detail in the sections that follow Note Ensure that you complete the plumbing tasks in the section Plumbing All NGC Systems on page 100 before continuing Table 9 Plumbing tasks for the NGC Quest system Task 1 Inlet tubing to the system pump A inlet m
38. all Inbound Rules for Bio Rad NGC Software 0 006 173 vi NGC Chromatography Systems and ChromLab Software 1 Introduction The NGC chromatography systems are preparative systems designed to rapidly automate the purification of biomolecules The flexible modular and economical design makes NGC the instrument of choice for method development and scale up It is available in six preplumbed factory tested configurations at two different flow rates Each preconfigured system can be further customized and upgraded by adding valves detectors or pumps in order to meet specific application needs Any system can be configured for either low flow rate or high flow rate operation by simply changing the pump head on the system pump modules As a result a single hardware platform can be modified as the application need and scale change ChromLab software enables you to control manage and evaluate the NGC system and the purification runs Installation Guide 7 1 Introduction Main Features NGC chromatography systems enable you to do the following Easily create purification and maintenance protocols from predefined method templates and protocol phases Automate multicolumn purification processes using preprogrammed templates and multiple column switching valves Automate multiple sample injections using either the sample inlet valve and the sample pump or the C 96 autosampler Expand sample monitoring using the
39. anifold 2 Inlet tubing to the system pump B inlet manifold 3 Tube 4 from the 20 psi backpressure regulator to the Common inlet port on the BioFrac fraction collector diverter valve The figure on page 107 shows the Point to Plumb connections for the NGC Quest system 106 NGC Chromatography Systems and ChromLab Software Plumbing NGC Systems NGC Quest Point to Plumb Connections SALT BUFFER AND BUFFER TO WAS BUFFER A BUFFER B Tubing Key Green PEEK F100 pumps Orange PEEK F10 pumps Clear FEP tubing Clear waste tubing O Point to Plumb connections Pre installed Installation Guide 107 5 Plumbing and Priming the NGC Chromatography Systems To plumb the system pumps 1 Connect inlet tubing to the inlet manifold of system pump A Outlet Priming port Inlet 2 Connect inlet tubing to the inlet manifold of system pump B 3 Immerse the open ends of both inlet tubing in a bottle of degassed deionized water To plumb the fraction collector 1 Connect Tube 4 to the open end of the backpressure regulator that you connected to the conductivity monitor in step 2 on page 105 2 Connect the other end of Tube 4 to the Common port on the BioFrac fraction collector diverter valve 108 NGC Chromatography Systems and ChromLab Software Plumbing NGC Systems Plumbing the NGC Scout System T
40. apped bubbles cycling sequentially through the ports Q1 to Q4 After the specified duration the pumps stop momentarily while the blending valve switches to port Q3 in order to flush the lines with water before closing the valve No change is allowed during this process for the specified duration Note You can stop the cycle before it completes by clicking Stop In this case ensure that all buffer lines are filled with the appropriate buffers before continuing Installation Guide 139 5 Plumbing and Priming the NGC Chromatography Systems To purge the buffer inlet valves 1 Open the System Pump dialog box and select Priming on the Mode dropdown list Note When in Priming mode the sample inject valve switches the system pump flow to waste W2 After the process is completed the sample inject valve switches the system pump to manually load the column through the loop 2 Select the first buffer port to purge for example Buffer A 1 3 Optional Change the default flow rate The default flow rate is set to 10 ml min 4 Click Start System Pump F10 Mode Priming Buffer Blending ports Select buffer blender port for priming qr OQ OG OQ Buffer Inlet ports Select inlet port for priming Inlet A D Inlet B 8 Bvpass 6 Dypass Duration 1 E Min Flow Rate 0 001 10 10 000 4 ml min D Start 5 Repeat steps 2 4 for the remaining buffer inlet ports 140 NGC Chrom
41. ated for 7 amps or more Table 4 Power requirements for the NGC instrument and the computer running ChromLab Parameter Mains input voltage NGC maximum power usage Number of power sockets Type of power sockets Requirement AC 100 240V 50 60 Hz lt 750 watts Minimum of three power sockets m One socket for the NGC instrument One socket for the computer running ChromLab software m One socket for the BioFrac fraction collector Asocket for any supported peripheral instrument that you attach for example the C 96 autosampler Tip The touch screen does not require a separate power supply IEC type connections grounded sockets Installation Guide 17 Preparing Your Site ChromLab System Requirements Bio Rad can provide a PC with ChromLab software preinstalled or you can install ChromLab onto a computer at your site Table 5 lists the minium system requirements for the computer running ChromLab known as the ChromLab computer Table 5 Minimum hardware requirements for the ChromLab computer Hardware Computer Operating system Processor RAM Hard disk space Required peripherals Monitor Screen resolution Screen colors Screen size Minimum requirement Either m Microsoft Windows 7 SP1 32 bit and 64 bit Microsoft Windows 8 32 bit and 64 bit Microsoft Windows 8 1 32 bit and 64 bit Intel dual core or equivalent 2 0 GHz 4 GB minimum 250 GB minimum 500 GB recommended DV
42. atography Systems and ChromLab Software Priming and Purging the System To purge the sample pump 1 In the fluidic scheme touch or click the Sample Pump module to open its dialog box 2 Inthe Sample Pump dialog box set Flow Rate to 10 ml min Sample Pump F100 3 ama 3 Flow Control Flow Rate 0 01 100 ini 5 ml min F Set Injection Volume 5S ml Pressure Limits Lower o 4 psi Upper 1450 psi Control Flow to avoid overpressure Inject Valve Inject Change Position D Start Enable Air Sensor Installation Guide 141 5 Plumbing and Priming the NGC Chromatography Systems 3 Click Change Position to set the sample inject valve to Sample Pump Waste and close its dialog box Sample Inject Valve 2 aA as Manual Load Loop System Pump to Column System Pump Inject Loop Sample Pump Waste Sample Pump Direct Inject System Pump Waste Sample Pump Load Loop System Pump to Column 4 Click Start To purge the sample inlet valve if available 1 In the fluidic scheme touch or click the Sample Pump module with the sample inlet valve to open its dialog box ve Ea 2 Inthe Sample Pump dialog box do one of the following m If only one sample inlet valve is present select S1 Port 8 as the port to purge If two sample inlet valves are present click Valve 2 and select S2 Port 8 as the port to purge 142 NGC Chromatography Sys
43. ave the settings and close the Power Options dialog box Installation Guide 19 2 Preparing Your Site To turn off automatic updates 1 Onthe ChromLab computer select Control Panel gt Windows Update and choose Change settings in the left pane 2 Under Important updates choose Never check for updates not recommended from the dropdown list 3 Click OK to save the setting and close the Change Settings window To change the font size 1 Onthe ChromLab computer select Control Panel gt Fonts and choose Change font size in the left pane 2 Select Smaller 100 default if it is not already selected and click Apply 3 Close the Display window 20 NGC Chromatography Systems and ChromLab Software 3 Setting Up NGC Chromatography Systems After you unpack the NGC chromatography system and install it in a standard cold cabinet or on the lab bench you will need to m Attach the touch screen Connect the drain tube to the buffer tray Connect the power and communication cables Connect the NGC system to the ChromLab computer This chapter explains how to perform these and other setup tasks Tip Before setting up the system familiarize yourself with the NGC system and its modules ports accessory kits cables and power switches Installation Guide 21 3 Setting Up NGC Chromatography Systems NGC Systems Accessories Kit Each NGC system ships with an accessories kit The contents of the ki
44. binet Caution Two or more individuals should lift the instrument gt Carefully lift the instrument into the cold cabinet See the packaging insert for information about lifting the NGC instrument To attach the touch screen to the touch screen stand 1 Place the touch screen monitor face down on a clean surface to prevent scratching 2 Using the 7 64 hex key provided in the accessories kit remove the cover from the back of the touch screen monitor Installation Guide 35 3 Setting Up NGC Chromatography Systems 3 Using a Phillips screwdriver remove the six screws that attach the touch screen to the pivot arm assembly and place them aside You will need them in step 6 4 Locate the touch screen mounting bracket in the NGC Touch Screen Mounting Stand kit 788 5040 36 NGC Chromatography Systems and ChromLab Software Attaching the Touch Screen 5 Position the bracket on the back of the touch screen so that the bracket opening is on the bottom You should see the cable connector port through the bracket opening 6 Attach the mounting bracket to the touch screen with the six screws that you set aside in step 3 Hole 1 Hole 2 Hole 3 Installation Guide 37 3 Setting Up NGC Chromatography Systems 7 Locate the four thumb screws in the packaging for the touch screen stand TTT 8 Using the four thumb screws loosely attach the touch screen bracket assembly to the stand T
45. c center divider Insert the top of the divider into its slot Gently bend the divider and slide the bottom of the divider up onto the lip or Ph ee NS Complete steps 6 11 in the section To insert or reposition modules on the NGC instrument Installation Guide 55 3 Setting Up NGC Chromatography Systems Connecting Peripheral Equipment This section includes information about Connecting the BioFrac Fraction Collector Connecting Column Clamps to the NGC Instrument Connecting a Keyboard or Mouse Connecting the Air Sensors Connecting the SIM to the NGC Instrument If you do not plan to connect these components to the NGC system you can skip this section Connecting the BioFrac Fraction Collector Tip For information about setting up and using the fraction collector see the BioFrac Fraction Collector User Guide The BioFrac fraction collector requires the communication adapter and its cable to connect to the NGC instrument ChromLab detects the fraction collector by way of the communication adapter To connect the BioFrac fraction collector to the NGC instrument 1 2 Ensure that the NGC instrument and the fraction collector are powered off Locate the connector port marked INSTRUMENT BUS on the back of the BioFrac fraction collector 56 NGC Chromatography Systems and ChromLab Software Connecting Peripheral Equipment 3 Insert system cable 17 that ships with the fraction collector into an INSTRUMENT BUS
46. c strips on both the right and left sides on which to mount column clamps air sensors and the fittings tightener The column clamps can be placed at any position on the strips The column clamps are adjustable and can accept a variety of column sizes up to 35 mm in diameter Tip For long columns use two column clamps to keep the columns from tipping A Caution Do not overtighten the clamps Glass columns can shatter if the clamps are overtightened Installation Guide 59 3 60 Setting Up NGC Chromatography Systems Connecting Tubing to the Columns Note Ensure that the system has been purged of air and that the tubing is filled with buffer before connecting the columns To connect tubing to the columns 1 Connect tubing from one of the following modules to the top of the column Sample inject valve column port Column switching valve inlet port 1 5 Note For best air free connection ensure that the tubing is dripping buffer at a flow rate of 0 5 1 ml min Fill the top of the column with buffer before inserting and tightening the fitting Remove the plug from the bottom of the column Connect the tubing from one of the following modules to the bottom of the column UV monitor Column switching valve outlet port 1 5 NGC Chromatography Systems and ChromLab Software Connecting Peripheral Equipment Connecting a Keyboard or Mouse The NGC systems can be controlled using the keyboard on the touch s
47. creen or by using a USB connected keyboard and mouse You can connect a keyboard and mouse to any of the four USB ports located on the right and left sides of the NGC instrument Soft power switch Ss lee rr T g Peripheral connector ports USB ports Installation Guide 61 3 Setting Up NGC Chromatography Systems Connecting the Air Sensors The system supports up to two air sensor modules and up to eight air sensors The air sensor module has a magnetic backing and can attach to the side of the NGC instrument Each module can contain up to four air sensors The system detects the number of air sensors that are attached to the instrument The air sensors can be set to detect air in lines connected to the system pumps to detect end of buffer They can also be set to detect air in lines connected to the sample pump to detect end of sample Two air sensors are available that differ in their internal diameter Large air sensors connect to 1 8 ID FEP tubing Smaller air sensors connect to 1 16 ID PEEK tubing Note The air sensor module accommodates both sizes simultaneously The air sensors can be inserted into the ports on the air sensor module ChromLab detects the presence or absence of air sensors in the module If you have fewer than four air sensors on the module you must cover the unoccupied ports with a protective blank cover that is provided with the module to prevent liquid from
48. ddress 169 254 251 226 3 Click Continue after ChromLab is connected to this system System Information Continue Shut down Note You might see a dialog box indicating that the system time is not synchronized to the computer time p om _ System time is not synchronized System time 11 19 2012 11 39 PM UTC 08 00 Pacific Time US amp Canada Local time 11 19 2012 12 39 PM UTC 08 00 Pacific Time US amp Canada Restart system as soon as possible to synchronize system time Restart System OK 90 NGC Chromatography Systems and ChromLab Software Establishing the Connection Between ChromLab Software and the NGC Systems Click Restart System to synchronize the system time with the ChromLab computer time Tip This is a one time action After the system time and the ChromLab computer time are synchronized the ChromLab computer manages the system time When ChromLab successfully establishes a connection you see the fluidic scheme for the configured NGC system on the touch screen man Li O O i i _ a Wy Pisave 2 A1 215 nm mau 3 Time Description Run Log Run Queue B Clear Autoscale S ResetZoom Annotate Manual Run O Z Legend 12151m 2255 nm 43 280 nm A4495 nm Conductivity xs pH System Pressure PreCol Pressure ACol Pressure Sample Pump Pressure Temnerature m Volume ml gt
49. ddress automatically are selected and click OK f Internet Protocol Version 4 TCP IPv4 Properties General Alternate Configuration You can get IP settings assigned automatically if your network supports this capability Otherwise you need to ask your network administrator for the appropriate IP settings Obtain an IP address automatically 5 Use the following IP address Obtain DNS server address automatically Use the following DNS server addresses Validate settings upon exit Close the Control Panel and open Network and Sharing Center Important ChromLab does not support third party firewall applications Uninstall or disable any third party firewall application to ensure that ChromLab functions correctly See Troubleshooting Network Connection Issues on page 158 for more information Installation Guide 157 B Troubleshooting ChromLab Software Installation Troubleshooting Network Connection Issues This section explains what to do if ChromLab is unable to connect to the NGC instrument because the IP address of the instrument has changed Locating the IP Address of the NGC Instrument The IP address of the NGC instrument is available on the initial connection screen as well as in the System Information dialog box IP Address on the ChromLab Connection Screen When you first start the ChromLab instrument the following dialog box appears
50. de of the instrument 3 Insert the connector cable on the air sensor module into one of the peripheral connector ports on the side of the NGC instrument 4 To connect an air sensor to the sample pump a Connect the tubing from the sample container to one port on the air sensor in the air sensor module b Connect the tubing from the other port on the air sensor to the inlet port of the sample pump 64 NGC Chromatography Systems and ChromLab Software Connecting Peripheral Equipment To connect an air sensor to a system pump a Connect tubing from the buffer bottle or from the outlet port on the one of the buffer inlet valves to one port on the air sensor b Connect tubing from the other port on the air sensor to the inlet port on the corresponding system pump To connect air sensors to the buffer blending valve a Connect tubing from each buffer bottle to one port on each air sensor that will be used b Connect tubing from the other port on each air sensor to the inlet ports on the buffer blending valve Alternatively you can attach tubing from the outlets A and B of the buffer blending valve to the top of the air sensors Connect the ports from the bottom of each air sensor to the inlets of system pumps A and B respectively In this setup although the air will be prevented from entering the system pumps and the system will react to end of buffer the system will n
51. delay volume when you install ChromLab You can change the delay volume when you change the tubing length or add other detectors along the flow path For more information about delay volume and event markers see the online help or the NGC Chromatography Systems and ChromLab Software User Guide To set the delay volume 1 Click File gt System Settings to open the System Settings dialog box 2 Choose the Delay Volume tab NGC Chromatography Systems and ChromLab Software Setting Delay Volume W System Settings NGC Device Output Air Sensors Email Notifications System Name Delay Volume _ Control Flow Remote Access Trace Settings Device Input v Synchronize with detector Tubing inner diameter 0 02 i J in V Include BioFrac 12 pl Tubing length between UV outlet port and BioFrac drophead Bl SL El Include Outlet valve 50 pl Tubing length b V outlet port and outlet valve Tubing length b outlet valve and BioFrac drophead Include analytical 5 mm UV flow cell volume 16 ul Include conductivity cell volume 6 pl J Include backpressure regulator 40 psi volume 80 pl E Include pH probe flow cell volume 200 il Inline 90 pl Bypass Additional volume 0 B pl Delay volume to BioFrac Heip OK Cancel Select Synchronize with detector Refer to the following table to specify the inner diameter of the tubing in use Tubing Color Inner Diameter
52. e modules that make up the NGC instrument and includes troubleshooting and maintenance information The NGC Chromatography Systems and ChromLab Software User Guide is available on your NGC Chromatography Systems Documentation DVD as a pdf file This guide explains how to use ChromLab software to set up and control the NGC instrument run protein separations and other operations manually program methods to automate purification runs evaluate the results and print experiment reports For ChromLab Help click the question mark in the upper right corner in dialog boxes to access relative information Screen level help is also available on the Help menu NGC video tutorials are available on the NGC Chromatography Systems Documentation DVD as mp4 files Tip You can click the Bio Rad logo in the upper left corner of any ChromLab window to launch the Bio Rad website 12 NGC Chromatography Systems and ChromLab Software 2 Preparing Your Site This chapter explains how to prepare your site to successfully install your NGC chromatography system and ChromLab software Delivery Crate Dimensions The crated NGC instrument is very heavy approximately 160 215 Ib 72 6 97 6 kg depending on the configuration Bio Rad strongly recommends that you use a pallet jack to move the instrument pallet from the shipping dock to the laboratory Table 1 Specifications for the NGC delivery crate Item NGC Quest NGC Quest Plus NGC
53. e required to update 4 min Device Name Actual Version Expected Version Gradient Pump 1 17 1 23 Gradient Pump Update Later Update Now A progress dialog box appears on the NGC touch screen with information about the update including the module that is being updated and the time remaining for that update Installation Guide 149 A Upgrading ChromLab Software and NGC Systems DO NOT TURN OFF SYSTEM 1 of 2 Updating Firmware of Gradient Pump A to version 1 17 Time Remaining 4 min 141 2805 packets 5 03 complete When the firmware update completes successfully the system reboots a second time and is then ready for use If the firmware update fails see Firmware Update Failure on page 163 150 NGC Chromatography Systems and ChromLab Software B Troubleshooting ChromLab Software Installation This appendix explains what to do if ChromLab software fails to connect to the NGC system Possible Causes for Connection Failures When ChromLab fails to connect to an NGC system the following error message appears r ChromLab e Failed to connect to system NGC SNO2000 Network address not reachable No such host is known Please check your network connections and verify the Network Name IP Address Retry Cancel Table 14 on page 152 lists several possible causes for the connection to fail Installation Guide 151 B 152 Troubl
54. eamrapran randana 7 MaikeatureS ranae ane e athe a a ara dhs A EE and a A gates ed 8 Salay IMIOMMALON ss she teas eaters a tread e wina Ee Sik Ree ek ed We atin 9 Saloy AlS 4 ccacecegav ev teweer ean geet eats bene le oe Regie 9 Salety PrecautlOns 2 45 dedv adage cigars Sk eda wea AEAN Oa eae Boe i 9 Regulatory Information 0 0 000 ec eee 10 Safely COMplanCe s4 0is00 28ers Oe haere aed eRe Se Red DARIN RES Ged 10 Electromagnetic Compatibility EMCO n saaana ananena 10 FCC Warning and Notes 0 0 cece ee 11 Finding OUt MOTE s esre Serta 2 bee a paces eats EEE EE ci ace hae arses 12 Chapter 2 Preparing Your Site 0 0 cece eee eee 13 Delivery Crate Dimensions 00 00 e eee 13 Room Requirements 0000 eee 15 Bench Spate cca via kee ee eR ae ee io amp eee w hed Pde ee eae 15 ENVIFONIMECHE easi a6 cre baee bee oes Rae Se bard eee She tea RHE RE Re Oe 16 Power Requirements ssu cusi rtra do aaea teeta 17 ChromLab System Requirements nuanua aaan aa naa 18 Preparing the ChromLab Computer 000000 eee eee eee 19 Chapter 3 Setting Up NGC Chromatography Systems 21 NGC Systems Accessories Kit 2 0 eee 22 NGC Scout Accessories Kit 2 0 0 cece eee 23 NGC Discover Accessories Kit 0 0 0 e eee 23 NGC Discover Pro Accessories Kit 0 0 0 0 00 cee eee 23 Installation Guide iii Table of Contents Attaching the Touch Screen se ssrarressrrisyi rit dd t
55. ecalibrate the pump flow rate and the conductivity and the pH monitors zero the pressure sensors and reset them to their factory settings Note You cannot run a method while calibrating the instrument Tip This section provides general procedures for calibrating the modules For more information about using ChromLab to calibrate the modules see the NGC Chromatography Systems and ChromLab Software User Guide Calibrating pH Use two pH calibration buffers with a difference of at least one pH unit Allow the buffers to reach the operating temperature before use To calibrate pH 1 On the NGC touch screen select Calibrate on the ChromLab dropdown menu 2 Select pH on the dropdown list 3 Follow the steps in the pH Calibration dialog box 4 Verify that Status at the bottom of the dialog box is Calibrated 144 NGC Chromatography Systems and ChromLab Software Calibrating the Instrument Calibrating the Pump Flow Rate Prime and purge the system pumps with water before starting the calibration procedure Calibrate the flow rate with water Note Ensure that the column is offline before performing this task To calibrate the pump flow rate 1 Onthe NGC touch screen select Calibrate on the ChromLab dropdown menu 2 Select Pump Flow Rate on the dropdown list 3 Follow the steps in the Pump Flow Rate Calibration dialog box 4 Verify that Status at the bottom of the dialog box is Calibrated Calibrating Pressure
56. ection HomeGroup Internet Options Choose homegroup and sharing options Windows Firewall Access files and printers located on other network computers or change sharing settings Z Troubleshoot problems Diagnose and repair network problems or get troubleshooting information eS 154 NGC Chromatography Systems and ChromLab Software Configuring the Network Adapter for Direct Connection Review the list of network connections If you see at least one icon labeled Local Area Connection proceed to step 4 GO gt Control Panel Network and Internet Network Connections 4 Seare Organize m Local Area Connection Wireless Network Connection m Wireless Network Connection 2 Mee Koota Be rosea Be Not conectes Intel R 82579LM Gigabit Network ill Intel R Centrino R Ultimate N 6 X ill Microsoft Virtual WiFi Miniport A Note If you do not see this icon Microsoft Windows does not recognize the network adapter This could be a driver problem with Windows Contact the vendor of the computer or your local IT department to solve this problem If the Local Area Connection icon is disabled right click the icon and select Enable to enable the network adapter A Local Area Connection A gt Disabled coy a Intel R Enable Status Diagnose Create Shortcut z Delete _ _ Rename W Properties Installation Guide
57. ed to use TCP IP 4 0 with dynamic host configuration protocol DHCP For information about configuring the network adapter see Configuring the Network Adapter for Direct Connection on page 154 78 NGC Chromatography Systems and ChromLab Software Connecting the NGC System to the ChromLab Computer Establishing a Network Connection When establishing a connection between the NGC system and the ChromLab computer through your network infrastructure ensure that they are both on the same subnet A DHCP server is required so that the system receives an IP address Note The DHCP server should be configured to always assign the same IP address to the system See your network administrator for more information Tip You should have already inserted the Cat 6 ethernet cable into the port at the back of the NGC instrument See To connect the power and communication cables on page 77 To establish a network connection gt Insert the loose end of the Cat 6 ethernet cable into the network connection router or hub Installation Guide 79 3 Setting Up NGC Chromatography Systems 80 NGC Chromatography Systems and ChromLab Software 4 Installing ChromLab Software This chapter explains how to install ChromLab software on your computer If this is the first time that ChromLab is installed on the computer the installation wizard determines whether the required versions of NET and SQL Server are installed If the required versions
58. eshooting ChromLab Software Installation Table 14 Possible Causes and Solutions for Connection Failures Possible Cause Solution The network name or IP address 1 entered is incorrect Verify that the NGC instrument is available on the network See Troubleshooting Network Connection Issues on page 158 for information about ensuring the NGC system is available on the network On the error message in ChromLab click Retry Enter the correct Network Name or IP Address of the instrument and click Connect see step 2 on page 87 NGC Chromatography Systems and ChromLab Software Possible Causes for Connection Failures Table 14 Possible Causes and Solutions for Connection Failures continued Possible Cause A firewall on the ChromLab 1 computer is turned on and does not allow the ChromLab programs and services to run Solution Check your firewall configuration and ensure that the required ChromLab programs and services are allowed to run See ChromLab Software Firewall Settings on page 162 for the list of required ChromLab programs and services See also Appendix D Firewall Configuration for information about configuring firewall applications and services On ChromLab Touch click Retry After the connection on the instrument is successful on the ChromLab computer click Retry Enter the correct Network Name or IP Address of the instrument and click Connect see step 2 on page 87 Installati
59. from the Out port on the conductivity monitor or pH monitor to the In port on outlet valve 1 01 b Optional Do one of the following Connect Tube 4 from port 1 on O1 to the Common inlet port on the BioFrac fraction collector diverter valve Connect Waste tubing to port 1 on O1 and immerse in a waste container c Connect tubing from port 12 on O1 to the In port on outlet valve 2 02 d Connect outlet tubing to all remaining outlet ports as needed 3 Immerse the outlet tubing in fraction collection containers 122 NGC Chromatography Systems and ChromLab Software Priming and Purging the System Priming and Purging the System This section explains how to prime your system before running the pumps and how to purge your system of air bubbles or rapidly exchanging buffer solutions Note This section assumes that you primed the wash system when you plumbed the system See Plumbing All NGC Systems on page 100 for more information Priming and purging tasks are specific to the NGC system Table 13 lists the tasks associated with each system configuration The tasks are explained in detail in the sections that follow Table 13 Priming and purging tasks for each NGC system configuration NGC Quest NGC Scout NGC Discover Prime and purge the inlet port on system pump A Prime and purge the inlet port on system pump B Prime and purge Q1 Q4 on the buffer blending valve Tip The inlet ports on s
60. g on the system Take care when lifting the instrument into the cold cabinet To reduce the risk of personal injury or damage to the instrument three or more people should perform this task If installing the NGC instrument into a standard cold cabinet a Lift the instrument into position b Connect the power cable to the power outlet Installation Guide 77 3 Setting Up NGC Chromatography Systems Connecting the NGC System to the ChromLab Computer Note Ensure that the ChromLab computer and the NGC instrument are powered off before beginning this task You can connect the NGC system to the ChromLab computer in one of two ways Direct cable connection using a Cat 6 ethernet cable Network connection through your network infrastructure Establishing a Direct Cable Connection Tip You should have already inserted the Cat 6 ethernet cable into the port at the back of the NGC instrument See To connect the power and communication cables on page 77 If the ChromLab computer has two network adapters and you are establishing a direct connection to the NGC instrument you can use the second adapter to connect the computer to your company s network To establish a direct cable connection gt Insert the loose end of the Cat 6 ethernet cable into one of the ethernet connection ports on the computer on which you plan to install ChromLab Note The network adapter that is used to connect the NGC instrument needs to be configur
61. hat your systems are powered off and unplugged before performing this task This section explains how to connect different external devices to the SIM Before connecting the device locate the connector ports that you will use on the back of the SIM Notice that the terminals differ for different devices To import analog signals to the NGC instrument connect the external device to either the SIM 1 or SIM 2 ports See the section To connect an external detector to the SIM 1 and SIM 2 ports that follows To export digital signals from the NGC instrument connect the external device to the DAC A port Optionally to trigger the external device to start measuring data from the NGC instrument connect a trigger wire to the DIGITAL OUT port See the section To connect an external device to the DAC A and DIGITAL OUT ports on page 74 Tip The SIM ships with its connectors inserted into their ports To connect an external detector to the SIM 1 and SIM 2 ports 1 On the back of the SIM firmly grasp the green SIM 1 or SIM 2 connector and pull it straight out of its port DAC DIGITAL OUT DIGITALIN SIM1 SIM 2 B 0 10V A GND fafa jesa amp ete ate Ett 1234GND GND GND 2 Locate the green flathead screwdriver included in the SIM s accessory kit Installation Guide 69 3 70 Setting Up NGC Chromatography Systems 3 Loosen all three screws on the top of the connector Tip T
62. he holes on the bay 52 NGC Chromatography Systems and ChromLab Software Inserting Additional Modules 10 Insert the screws that shipped with the module into the screw holes Caution Do not overtighten the screws 11 Tighten the screws on the front of the module Converting Bays to Fit Modules Some modules fit into single wide bays while others require double wide bays Such as the system and sample pump modules and the UV and UV Vis detector modules Bays can be converted from one size to the other by adding or removing the center divider The following image shows two adjacent empty single wide bays Installation Guide 53 3 Setting Up NGC Chromatography Systems To convert a single wide bay to a double wide bay 1 Complete steps 1 4 in the section To insert or reposition modules on the NGC instrument on page 51 2 Gently lift the divider upward off of the lip to unhook it and then pull it out of the instrument Slot for divider tip Tip of divider Bottom of divider Divider lip 3 Store the divider in a safe place for later use 4 Complete steps 6 11 in the section To insert or reposition modules on the NGC instrument 54 NGC Chromatography Systems and ChromLab Software Inserting Additional Modules To convert a double wide bay to a single wide bay 1 Complete steps 1 4 in the section To insert or reposition modules on the NGC instrument on page 51 Locate a plasti
63. he standard cold cabinet it is recommended that you connect the power and communication cables before lifting the instrument into the cabinet Note The Cat 6 ethernet cable to connect the NGC instrument to the computer running ChromLab software is approximately 10 3 0 m in length ml j p 8 I S 6 LEGEND 1 Power on off switch 2 Fuse cover 3 Power inlet port 4 Power outlet ports 5 Ethernet connection port 6 Touch screen connection port 76 NGC Chromatography Systems and ChromLab Software Connecting the Power and Communication Cables To connect the power and communication cables i If you have not already done so insert the end of the touch screen cable into the touch screen connection port on the back of the instrument Insert the Cat 6 or higher cable into the ethernet port on the back of the instrument Optional Connect the power cords for any peripheral instruments into the power outlet ports Connect the power cable to the power inlet port If installing the NGC instrument into a standard cold cabinet direct the following cables through the access port on the side of the cabinet m Touch screen cable m Ethernet cable m Power cable if your cabinet does not have a power outlet Press the I O power switch to the On position Caution The weight of the NGC instrument varies from moderate to heavy dependin
64. hen tighten the thumb screws evenly after you have completed the initial mounting 9 Place the mounted touch screen on the table outside the cold cabinet 10 Locate the end of the touch screen display cable that you routed through the access port 11 Firmly push the touch screen cable into the port at the bottom of the touch screen mounting bracket and tighten the thumbscrews 38 NGC Chromatography Systems and ChromLab Software Connecting the Drain Tube to the Buffer Tray Connecting the Drain Tube to the Buffer Tray The top of the NGC system is molded to contain the buffer tray and route its drain tube to the right or left side of the instrument The drain tube connects to the buffer tray through a connector on the back of the instrument top Note The drain tube connector is barbed to keep the tube tightly in position After it is securely attached to the connector the tube will be very difficult to remove Plan your setup carefully before attaching the drain tube to the connector To connect the drain tube to the buffer tray 1 Locate the instrument top and buffer tray 2 Place the top upside down on the lab counter with the back side of the tray facing toward you 3 Determine the side of the instrument on which to place your waste container and then locate the corresponding right or left drain tube hole in the top Installation Guide 39 3 Setting Up NGC Chromatography Systems 4 Uncoil the d
65. hese screws adjust the terminals that hold the wires in place The screws do not dislodge from the connector Left or positive screw Right or ground screw Middle or negative screw Locate the analog output cable that ships with your detector If connectors are attached to both ends of the cable remove the connector that does not insert into your detector Tip Alternatively contact the manufacturer for a suitable cable If necessary strip the plastic wire coating off the wires so that they are exposed Obtain a small piece of 20 24 AWG negative wire Bend the wire into a U shape and strip the plastic coating off both ends to create a jumper wire NGC Chromatography Systems and ChromLab Software Connecting Peripheral Equipment 9 On the analog output cable determine the positive and negative wires 10 Insert the positive wire into the left terminal on the connector and tighten the screw Note Take care not to overtighten or fray the wire Left or positive terminal Right or ground terminal Middle or negative terminal Installation Guide 71 3 Setting Up NGC Chromatography Systems 11 Insert the negative wire and one end of the jumper wire into the middle terminal on the connector and tighten the screw i 12 Insert the other end of the jumper wire into the right or ground terminal on the connector and tighten the screw 72 NGC Chromatography Systems and Chro
66. his may take several minutes Status InstallShield Note Installing ChromLab can take several minutes When the installation is complete click Finish to exit the wizard InstallShield Wizard Completed The InstallShield Wizard has successfully installed ChromLab Software Click Finish to exit the wizard lt Back Eenh cancel Installation Guide 83 4 Installing ChromLab Software Establishing the Connection Between ChromLab Software and the NGC Systems Verify that you completed the following tasks Connected the touch screen to the NGC instrument Connected all required peripheral equipment including o BioFrac fraction collector o Air sensor module if used o C 96 autosampler if used Connected the NGC instrument to the ChromLab computer Installed ChromLab on the ChromLab computer Powering on the NGC Instrument and Peripherals To power on the NGC instrument and attached peripheral equipment 1 Connect a power cable to the power outlet on the back of the NGC instrument and to the main power outlet 2 Press the power switch on the back of the NGC instrument to the ON position 3 Power on the attached peripheral equipment specifically m BioFrac fraction collector Note You must power on the BioFrac fraction collector before powering on the NGC system so that the fraction collector is recognized during system setup C 96 autosampler if used 84 NGC Chromatography S
67. hose appliances The user should be aware of this potential and take appropriate measures to avoid interference The NGC instrument should not be modified or altered in any way Alteration of the NGC instrument will Void the manufacturer s warranty Void the IEC EN UL CSA 61010 1 safety compliance Create a potential safety hazard Bio Rad Laboratories is not responsible for any injury or damage caused by the use of this instrument for purposes other than those for which it is intended or by modifications of the instrument not performed by Bio Rad Laboratories or an authorized agent Important The NGC instrument is intended for laboratory use only We strongly recommend that you follow the safety specifications listed throughout this manual Use only the supplied power cord with the instrument making sure to choose the plug adapter that corresponds to the electrical outlets in your region Installation Guide 11 1 Introduction Finding Out More After you install NGC documentation from the NGC Chromatography Systems Documentation DVD you can access installed NGC guides and tutorials on the Help menu in any ChromLab view More information about the NGC chromatography systems and ChromLab software is available from the following sources The NGC Chromatography Systems and ChromLab Software Instrument Guide is available on your NGC Chromatography Systems Documentation DVD as a pdf file This illustrated guide details th
68. iming Buffer Blending ports uffer blender port for priming 02 Q Buffer Inlet ports Select inlet port for priming Duration 15 Min Flow Rate 0 001 10 10 000 ml min D Start 4 Insert a 30 ml syringe into the priming port on system pump A 5 Turn the priming port valve counter clockwise one full turn to open the seal 6 Slowly withdraw the plunger to withdraw liquid from the port until air no longer enters the syringe approximately 10 ml and all the tubing is filled with liquid 7 Turn the priming port valve clockwise to close the port 8 Remove the syringe from the priming port and discard the liquid into waste 134 NGC Chromatography Systems and ChromLab Software Priming and Purging the System 9 Repeat steps 4 8 for the remaining buffer inlet ports 10 To prime buffer inlet valve B select buffer B1 and perform steps 3 9 on system pump B To prime the sample pump 1 Immerse the inlet tubing for the sample pump in the appropriate container 2 Insert a 30 ml syringe into the priming port 3 Turn the priming port valve counter clockwise one full turn to open the seal Outlet Priming port Inlet 4 Slowly withdraw the plunger to withdraw liquid from the port until air no longer enters the syringe approximately 10 ml and all tubing is filled with liquid 5 Turn the priming port valve clockwise to close the port 6 Remove the syringe from the pri
69. ing the NGC Chromatography Systems 3 To purge all lines of air bubbles and fill them with buffer a Click All b Optional Change the default flow rate The default flow rate is set to 20 ml min c Click Start The system pumps and the buffer blending valve start operating at the specified flow rate exchanging the solution in the inlet lines and expelling any trapped bubbles cycling sequentially through the ports Q1 to Q4 After the specified duration the pumps stop momentarily while the blending valve switches to port Q3 in order to flush the lines with water before closing the valve No change is allowed during this process for the specified duration Note You can stop the cycle before it completes by clicking Stop In this case ensure that all buffer lines are filled with the appropriate buffers before continuing 130 NGC Chromatography Systems and ChromLab Software Priming and Purging the System Priming and Purging the NGC Discover System Priming the NGC Discover system is a manual procedure and involves priming the buffer blending valve starting from the Q4 port to Q1 then the buffer inlet valve starting from buffer inlet A1 Purging the NGC Discover system is an automated procedure and involves removing air from one or all buffer lines as well as the buffer inlet valves and the system and sample pumps Both procedures require access to ChromLab Tip You can perform the steps on the NGC touch screen On the NGC Disco
70. iriti ERER PEISA 24 NGC System Location Is on a Lab Bench 00 000 eee eee 24 NGC System Location Is a Standard Cold Cabinet 2000505 34 Connecting the Drain Tube to the Buffer Tray 00 00 39 Installing the pH Probe in the pH Module 000 cece eee eee 42 Attaching an Expansion Tier to the NGC Instrument 00005 44 Inserting Additional Modules 0 0 0 eee ees 51 Connecting Peripheral Equipment 000 0c eee 56 Connecting the BioFrac Fraction Collector 0 00 eee eee eee 56 Connecting Column Clamps to the NGC Instrument 2 59 Connecting Tubing to the Columns 0000 naaa 60 Connecting a Keyboard or Mouse 0000 00 eee eee eee 61 Connecting the Air Sensors 1 2 0 0 ee 62 Connecting External Devices to the NGC Instrument 66 Connecting the Power and Communication Cables 000 76 Connecting the NGC System to the ChromLab Computer 78 Establishing a Direct Cable Connection 0 00 0 e eee eee 78 Establishing a Network Connection 0 00 e eee 79 Chapter 4 Installing ChromLab Software 00000000 81 Installing ChromLab Software 1 0 0 cee ee 82 Establishing the Connection Between ChromLab Software and the NGC Systems 0 00 cece eee 84 Powering on the NGC Instrument and Peripherals 00 84 Setting the
71. let tubing in a bottle of degassed deionized water Connect outlet tubing to the Sample Pump port on the sample inject valve NGC Chromatography Systems and ChromLab Software Plumbing NGC Systems To plumb the pH valve 1 Connect the pump head wash priming tubing to the Cal In port on the pH valve 2 Connect waste tubing to the Cal Out port on the pH valve Calibration Outlet to waste port fraction collector Calibration A Inlet from injection port e e conductivity Cal In In flow cell 3 Immerse the other end in the waste container 4 Connect Tube 2 from the 20 psi backpressure regulator that you connected in step 2 on page 105 to the inlet port In on the pH valve 5 Connect Tube 4 to the outlet port Out on the pH valve 6 Connect the other end of Tube 4 to the Common port on the BioFrac fraction collector diverter valve Installation Guide 117 5 118 Plumbing and Priming the NGC Chromatography Systems Plumbing the NGC Discover Pro System Table 12 lists the tasks for plumbing the NGC Discover Pro system The tasks are explained in detail in the sections that follow Note Ensure the you complete the plumbing tasks in the sections Plumbing All NGC Systems on page 100 and Plumbing the NGC Discover System on page 113 before continuing Table 12 Plumbing tasks for the NGC Discovery Pro system Tasks Tube 6 between the outlet port on the sample inlet valve and the inlet p
72. mLab Software Connecting Peripheral Equipment 13 Insert the connector into the SIM 1 or SIM 2 port on the back of the SIM DAC DIGITALOUT DIGITALIN SIM1 SM2 BO 10VA F GND GND GND4327 ASACGNDMEACNDEEEG 14 Refer to the chapter System Controls in the NGC Chromatography Systems and ChromLab Software User Guide for information about how to enable signal import from the device Installation Guide 73 3 Setting Up NGC Chromatography Systems To connect an external device to the DAC A and DIGITAL OUT ports 1 On the back of the SIM firmly grasp the DAC A connector and pull it straight out of its port Note ChromLab supports solely the DAC A port When connecting an analog instrument use the DAC port on the right DAC A DAC DIGITAL OUT DIGITALIN SIM1 SIM 2 BO 10VA GND GND GND 4321 1234GND GND GND a Ss 2 Optional If you want the NGC instrument to trigger the device to start measuring data also remove the DIGITAL OUT connector and perform the steps that follow 3 Locate the green flathead screwdriver included in the SIM s accessory kit 4 Loosen the positive ground and terminal 2 screws on the connectors Note Terminal 1 on the DIGITAL OUT connector is reserved for the autosampler Do not insert the trigger wire into terminal 1 Terminal 2 is the second screw from the right 5 Locate the outpu
73. ming port and discard the liquid into waste Installation Guide 135 5 Plumbing and Priming the NGC Chromatography Systems To prime the sample inlet valve if available 1 Immerse the tubing for the inlet valve in the appropriate buffer container 2 Onthe ChromLab computer or the touch screen start ChromLab in Manual mode 3 In the fluidic scheme touch or click the Sample Pump module with the sample inlet valve to open its dialog box 4 Inthe Sample Pump dialog box select the first sample inlet port to prime Sample Pump F100 2 e ae Select Sample Inlet Port St Pots Flow Control Flow Rate 0 01 100 ml min E Set Injection Volume Pressure Limits Lower 0 4 psi Upper 1450 EA psi E Control Flow to avoid overpressure Inject Valve Waste Change Position gt Start V Enable Air Sensor 136 NGC Chromatography Systems and ChromLab Software Priming and Purging the System Insert a 30 ml syringe into the sample pump s priming port Slowly withdraw the plunger to withdraw liquid from the port until air no longer enters the syringe approximately 10 ml Remove the syringe from the priming port and discard the liquid into waste Repeat steps 4 7 for the remaining sample inlet ports Installation Guide 137 5 Plumbing and Priming the NGC Chromatography Systems Purging the NGC Discover System To purge the buffer blending valve
74. ml The signal import module SIM connects the C 96 autosampler to NGC systems The SIM digitizes the analog signal from the C 96 autosampler and transmits the signal to the NGC system This appendix explains how to connect and set up the C 96 autosampler for use with the NGC instrument Tip To install and set up the C 96 autosampler see its documentation Connecting the C 96 Autosampler to the NGC Instrument To connect the autosampler to the NGC instrument 1 Ensure that both the NGC instrument and the C 96 autosampler are powered off 2 Connect the SIM device to the NGC instrument See Connecting the SIM to the NGC Instrument on page 67 for more information 3 On the back of the SIM firmly grasp the DIGITAL IN female connector and pull it straight out of its port 4 Grasp the DIGITAL OUT male connector and pull it out of its port Installation Guide 165 C Connecting the C 96 Autosampler to NGC Systems 5 Locate the two cables that connect the autosampler to the SIM 15 pin D connector cable part 10027227 9 pin D connector cable part 10027224 These cables ship with the autosampler Attach the DIGITAL IN connector to the 15 pin D connector cable Tip The 15 pin D connector cable has three wires red black and brown a From left to right loosen the first second and last screws on the connector b If necessary strip the plastic wire coating off the wires so that they are exposed
75. nstrument and the autosampler 168 NGC Chromatography Systems and ChromLab Software Using the C 96 Autosampler with NGC Systems To prime and purge the C 96 autosampler Note Before priming the autosampler ensure that any column is offline 1 Immerse the inlet tubing in a container of buffer or HPLC grade filtered degassed or other high quality water Use a syringe to prime the pumps See the section Priming and Purging the System on page 123 for specific information On the ChromLab computer or the touch screen start ChromLab in Manual mode Set the fluidic scheme to display the autosampler and sample inject valve Autosampler A Sample Inject valve In the fluidic scheme touch or click the Sample Inject Valve module to open its dialog box Installation Guide 169 C Connecting the C 96 Autosampler to NGC Systems 6 Set the inject valve to System Pump Inject Loop Sample Inject Valve 2 ual 3 Manual Load Loop System Pump to Column System Pump Inject Loop System Pump Waste 7 Inthe fluidic scheme touch or click the System Pump module to open its dialog box 8 Inthe System Pump dialog box a Set Flow Rate to 5 ml min b Optional Set Duration to at least 2 min c Click Start 170 NGC Chromatography Systems and ChromLab Software Using the C 96 Autosampler with NGC Systems System Pump F10 qe w Flow Control Flow Rate 0 001 10 5 000
76. o Point 000 cee eee eee 145 Calibrating the Conductivity Monitor 0 0 2 0 0 0 eee ee 146 Appendix A Upgrading ChromLab Software and NGC Systems 147 Upgrading ChromLab Software snis i ere ei e eae aia E E 147 Upgrading ChromLab Software to the Current Version 147 Resetting Customized System Settings 00 e eee eee 148 Updating NGC Systems 0 0 0 0 cee eee 148 Installation Guide v Table of Contents Appendix B Troubleshooting ChromLab Software Installation 151 Possible Causes for Connection Failures 0 000 eee 151 Configuring the Network Adapter for Direct Connection 154 Troubleshooting Network Connection IssueS 0 0000 e eee eee 158 Locating the IP Address of the NGC Instrument 000 158 Checking the Network Connection 0 000 e eet ee 160 ChromLab Software Firewall Settings 0 00 0 e eee eee 162 Firmware Update Failure 000 0 163 Appendix C Connecting the C 96 Autosampler to NGC Systems 264 eh ch cciwivetiost oS hoc eee eee eee es 165 Using the C 96 Autosampler with NGC Systems 02000 eae 165 Connecting the C 96 Autosampler to the NGC Instrument 165 Plumbing and Priming the C 96 Autosampler 00 200 eae 168 Setting Up the C 96 Autosampler for Use in ChromLab Methods 172 Appendix D Firewall Configuration 00000eeeeee 173 Firew
77. o the upper tier s connector port Tip You hear a click when the connector is properly seated 48 NGC Chromatography Systems and ChromLab Software Attaching an Expansion Tier to the NGC Instrument 10 Insert the other connector into the lower tier s connector port _ 11 Place the top with the buffer tray on top of the NGC instrument and place the buffer bottles into the buffer tray 12 Insert tubing lines into the sample and buffer bottles Installation Guide 49 3 50 Setting Up NGC Chromatography Systems 13 Perform the following tasks Task Insert modules into the new tier Plumb the system Start the instrument Prime the system Calibrate the system Refer to the section Inserting Additional Modules on page 51 Plumbing NGC Systems on page 99 Powering on the NGC Instrument and Peripherals on page 84 Priming and Purging the System on page 123 Calibrating the Instrument on page 144 NGC Chromatography Systems and ChromLab Software Inserting Additional Modules Inserting Additional Modules A A WARNING Disconnect power to the NGC instrument before inserting or removing any module No user serviceable parts are inside any component unless noted in this manual Refer servicing questions to Bio Rad service personnel WARNING To reduce the chance of liquid seeping into the instrument all open bays must be filled with at least an empty module The NGC instrument will n
78. ode 5 In ChromLab complete the method setup and start the run For multiple samples perform a multi run of the ChromLab method The number of runs in the multi run should correspond to the total number of injections from the same vial or multiple vials to be programmed on the autosampler 172 NGC Chromatography Systems and ChromLab Software D Firewall Configuration Firewall Inbound Rules for Bio Rad NGC Software Bio Rad configures the ChromLab software using Microsoft Windows firewall with advanced security If you have a third party firewall application you must configure that software so that it meets Bio Rad software s firewall rules The rules for NGC are listed in Table 15 on page 174 You can see the complete list of inbound firewall rules in the Windows Firewall with Advanced Security tool Note If you have antivirus software installed on your ChromLab computer ensure that it is configured to allow the Bio Rad NGC networking applications and services listed in this table To view Bio Rad NGC Firewall Inbound Rules 1 Onthe ChromLab computer open the Control Panel and select Administrative Tools 2 Inthe list of tools double click Windows Firewall with Advanced Security 3 In the left pane select Inbound Rules The list of inbound rules appears in the middle pane Tip If the list is long sort the list alphabetically to view the rules for Bio Rad NGC Important Ensure that the settings for you
79. on Guide 153 B Troubleshooting ChromLab Software Installation Configuring the Network Adapter for Direct Connection When connecting the ChromLab computer to the NGC system through a direct cable connection the network adapter on the ChromLab computer must be configured to use TCP IP 4 0 with DHCP This section explains how to configure Local Area Connection on the ChromLab computer To configure Local Area Connection 1 To verify the correct setup open the Control Panel and open Network and Sharing Center 2 In the Network and Sharing Center click Change adapter settings in the left pane k G6 gt Control Panel All Control Panel Items Network and Sharing Center x 4 Search Control Pane P Control Panel Home A i A r j View your basic network information and set up connections Manage wireless networks ky eo See full map Chi dapter setti ange adapter settings This computer Root AD Internet Change advanced sharing aF View your active networks Connect or disconnect Access type Internet Root AD Connections J Local Area Connection Domain network atl Wireless Network Connection Change your networking settings F g Set up a new connection or network Set up a wireless broadband dial up ad hoc or VPN connection or set up a router or access point See al fy Connect to a network See also Connect or reconnect to a wireless wired dial up or VPN network conn
80. ontrol and Laboratory Use m EC 61010 1 Safety Requirements for Measurement Control and Laboratory Use Part 1 General Requirements cTUVus Mark m UL STD No 61010 1 Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements m CAN CSA C22 2 No 61010 1 12 Safety Requirements for Measurement Control and Laboratory Use Part 1 General Requirements includes Amendment 1 Products with these safety compliance marks are safe to use when operated in accordance with the instruction manual This does not extend to accessories with no marks even when used with this unit Electromagnetic Compatibility EMC CE Mark m EN61326 Class A Electrical Equipment for Measurement Control and Laboratory Use General Requirements The NGC instrument is designed to operate in a controlled electromagnetic environment Do not use radio transmitters within 10 ft 3 m of the instrument 10 NGC Chromatography Systems and ChromLab Software Regulatory Information FCC Warning and Notes The NGC instrument complies with part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation It is possible that emissions from the NGC instrument may interfere with some sensitive appliances when placed nearby or on the same circuit as t
81. ort on the sample pump Note Remove the inlet tubing from the sample pump inlet manifold if it is installed Connect one of the following m Tube 4 from the Common inlet port on the BioFrac fraction collector diverter valve to port 1 on the outlet valve Waste tubing to port 1 on the outlet valve Inlet tubing to the remaining ports on the sample inlet valve See the section Plumbing Sample Inlet Valves on page 120 Outlet tubing to the remaining ports on the outlet valve See the section Plumbing Outlet Valves on page 121 The figure on page 119 shows the Point to Plumb connections for the NGC Discover Pro system NGC Chromatography Systems and ChromLab Software Plumbing NGC Systems NGC Discover Pro Point to Plumb Connections Q3 Q2 TUTE TO WASTE TO WASTE Tubing Key Green PEEK F100 pumps Orange PEEK F10 pumps Clear FEP tubing Clear waste tubing O Point to Plumb connections Pre installed Installation Guide 119 5 Plumbing and Priming the NGC Chromatography Systems Plumbing Sample Inlet Valves Each sample inlet valve module includes all necessary tubing The inlet tubing is approximately 5 1 5 m in length Determine the length of inlet tubing you need and cut the tubing to the appropriate length Note NGC inlet valves can be used for buffer or sample selection To plumb the sample inlet valves 1 If you
82. ot distinguish which of the four ports needs replenishing To activate the air sensors 1 Start ChromLab The green LEDs on the air sensor module should turn on Select File gt System Settings to open the System Settings dialog box Select the Air Sensors tab By default only the air sensors that the system detects are enabled The other sensors are disabled For each air sensor that you want activate select its checkbox and from its dropdown list choose either Sample to detect end of sample or Buffer to detect end of buffer Click OK Installation Guide 65 3 Setting Up NGC Chromatography Systems Connecting External Devices to the NGC Instrument Tip If you do not plan to connect the C 96 autosampler or other external devices to the NGC instrument you can skip this section This section explains how to connect the signal import module SIM to the NGC instrument and how to connect external devices to the SIM m For information about connecting the C 96 autosampler to the SIM see Appendix C Connecting the C 96 Autosampler to NGC Systems on page 165 For information about connecting the SIM to an NGC instrument see Connecting the SIM to the NGC Instrument on page 67 For information about connecting other external devices to the SIM see Connecting External Devices to the SIM on page 69 Next see the chapter System Controls in the NGC Chromatography Systems and ChromLab Software User Guide to enable the
83. ot operate if it detects a slot without a module inserted To insert or reposition modules on the NGC instrument 1 Ensure that the NGC instrument is powered off 2 Loosen the screws on the front of the module cover or covers 3 Firmly grasp both captive screws and pull the module forward out of its bay The following image shows an empty bay z ZH KN 4 Store the module and the screws in a safe place for future use Installation Guide 51 3 Setting Up NGC Chromatography Systems 5 Optional Convert the bay to a single wide bay or a double wide bay See Converting Bays to Fit Modules on page 53 6 Locate the module that you plan to install and remove it from its packaging 7 The frame of the bay has tracks on the top and bottom of the right side The module has guide rails on the top and bottom right side Align the rails on the module with its track in the bay to properly guide it into position Top of Module Bottom of Module Track on top right of bay Rail on top right a of module l MIXER e _e Rail on bottom right of module Track on bottom right of bay 8 Place the module into the open bay and gently slide it in as far as it will go Note Each module has an alignment pin on the back to ensure that it aligns correctly with the main communication board 9 Ensure that the screw holes on the module align with t
84. p head wash inlet to the 1 4 28 union attached to the pump head wash priming line on system pump B 5 On the sample inject valve Sample loop m Injection port Waste lines 2 6 Tube 2 from the outlet on the conductivity flow cell to the inlet on the 20 psi backpressure regulator Plumbing the System Pumps Wash Lines All configurations ship with the pump wash system preplumbed except for the inlet and outlet tubing for the pump head wash Plumbing the inlet and outlet tubing is specific to the type of pump system pump configuration or sample pump The following images show a detailed view of the connectivity and flow direction of the rinse solution through the tubing segments for the system pumps and the sample pump NGC Chromatography Systems and ChromLab Software Plumbing NGC Systems System Pumps Wash Lines Rinse fluid outlet from pump System pump A System pump B Rinse fluid inlet to pump Sample Pump Wash Line Rinse fluid outlet from pump Rinse fluid inlet to pump Sample pump Installation Guide 101 5 Plumbing and Priming the NGC Chromatography Systems To plumb and prime the system pump wash lines ls Mount a pump head wash tube holder to the right or left side of the instrument in either the upper or lower rack slots Fill a 50 ml tube with 20 isopropanol IPA and insert it in the tube holder Tip You can use 20 ethanol as an alternative Connect inlet t
85. position its pivot arm assembly before mounting the touch screen onto the mounting bracket To reposition the touch screen s pivot arm assembly 1 Place the touch screen monitor face down on a clean surface to prevent scratching 2 Using the 7 64 hex key provided in the accessories kit remove the cover from the back of the touch screen monitor Installation Guide 31 3 Setting Up NGC Chromatography Systems 3 Using a Phillips screwdriver remove the six screws that attach the touch screen to the pivot arm assembly and place them aside 4 Lift the pivot arm assembly off of the touch screen The mounting assembly should be in your right hand 5 Turn the pivot arm assembly around 180 so that the mounting assembly is now in your left hand and insert the assembly into its position on the back of the touch screen 6 Using the six screws that you removed in step 3 reattach the pivot arm assembly to the touch screen with a Phillips screwdriver 7 Reattach the cover to the back of the touch screen with the 7 64 hex key 8 Pivot the touch screen so that the screen faces forward and the cable connector is at the bottom of the screen 32 NGC Chromatography Systems and ChromLab Software Attaching the Touch Screen 9 After you attach the mounting post to the side of the instrument slide the touch screen display onto it 10 Insert a 4 20 x 34 hex bolt underneath the mount to secure the
86. r system has only one sample inlet valve a Connect tubing from the Out port on the sample inlet valve to the sample pump inlet manifold b Connect inlet tubing to ports 1 8 as needed 120 NGC Chromatography Systems and ChromLab Software Plumbing NGC Systems 2 If your system has two sample inlet valves a Connect tubing from the Out port on sample inlet valve 1 S1 to the sample pump inlet manifold b Connect tubing from the Out port on sample inlet valve 2 S2 to port 8 on S1 c Connect inlet tubing to ports 1 7 on S1 and to ports 1 8 on S2 as needed 3 Immerse the inlet tubing in sample containers Plumbing Outlet Valves Each outlet valve module includes all necessary tubing The tubing is approximately 5 1 5 m in length Determine the length of tubing you need and cut it to the appropriate length To plumb the outlet valves 1 If your system has only one outlet valve a Connect tubing from the Out port on the conductivity monitor or pH monitor to the In port on the outlet valve Installation Guide 121 5 Plumbing and Priming the NGC Chromatography Systems b Optional Do one of the following Connect Tube 4 from port 1 to the Common inlet port on the BioFrac fraction collector diverter valve Connect Waste tubing to port 1 and immerse in a waste container c Connect outlet tubing to the remaining ports as needed 2 If your system has two outlet valves a Connect tubing
87. r third party tool follow the rules for Bio Rad NGC If your tool has a Trust Network or similar setting ensure that it is enabled allowing an unrestricted trusted connection between the ChromLab computer and the NGC instrument Installation Guide 173 D Firewall Configuration For all services and applications listed in Table 15 the following rules apply Enabled Yes Action Allow Allowed Users Any Table 15 Firewall inbound rules for Bio Rad NGC software Name Group Program Protocol Required Bio Rad Networking Applications and Services Bio Rad NGC ICMPv4 Bio Rad NGC Network Discovery Bio Rad NGC SQLServer Browser Bio Rad NGC SQLServer NG Bio Rad NGC Time Source Bio Rad NGC ChromLab Application Any Any Any Any Any ICMPv4 UDP Any Any Any Any 174 NGC Chromatography Systems and ChromLab Software Allowed Computers Any Local Port Any 5355 Any Any Any Any Remote Port Any Any Any Any Any Any Life Science Group 10026252 RevD US EG Bio Rad Laboratories Inc Web site www bio rad com USA 800 424 6723 Australia 61 2 9914 2800 Austria 43 1 877 89 01 Belgium 03 710 53 00 Brazil 55 11 3065 7550 Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00 France 01 47 95 69 65 Germany 49 89 31 8840 Greece 30 210 9532 220 Hong Kong 852 2789 3300 Hungary 36 1 459 610
88. radio button enter the system s name or IP address and click Connect Tip This is the information that you noted on ChromLab Touch in step 2 on page 87 The IP address for an NGC instrument that is directly connected to the ChromLab computer begins with the number sequence 169 254 NGC Chromatography Systems and ChromLab Software Establishing the Connection Between ChromLab Software and the NGC Systems 3 Onthe NGC instrument you see that the system is attempting to connect to the ChromLab computer Please wait while the system connection is being established 4 When ChromLab successfully connects to the instrument you see CONNECTED to lt System_name gt at the bottom of the System Control pane Method Editor Calibration CONNECTED to NGC Failed to connect to system NGC SN02000 Network address not reachable No such host is known Please check your network connections and verify the Network Name IP Address Installation Guide 89 4 Installing ChromLab Software There are several possible causes for connection to fail See Appendix B Troubleshooting ChromLab Software Installation for more information and possible solutions On the NGC system gt On the touch screen click Continue Connect ChromLab To System 1 Launch ChromLab software on the connected computer 2 Connect to this system using the following Network Name NGC SN020007 IP A
89. rain tube and insert it through the drain hole on the side of the top A Caution Ensure that no kinks are visible in the tube before connecting it to the connector 5 Grasp the interior end of the tube and push it onto the drain connector as far back as possible to secure it in position The tube should rest in the interior notch and be positioned so that it bends according to its natural coil 40 NGC Chromatography Systems and ChromLab Software Connecting the Drain Tube to the Buffer Tray 6 Turn the top right side up and place it on the NGC instrument 7 Place the buffer tray on the top of the instrument and slide it all the way back until the black gasket is securely fixed to the front of the drain connector Installation Guide 41 3 Setting Up NGC Chromatography Systems Installing the pH Probe in the pH Module Note This procedure assumes that the pH module is already installed in your system and the tubing is already connected to the appropriate positions The pH probe is packaged in storage solution to protect it during shipping To install the pH probe l S g i oN Remove the cap from the pH valve flow cell Add a small amount of water 2 ml to the pH valve flow cell Remove the pH probe from the storage solution Inspect the probe to determine if the air bubble in the stem has moved into the bulb area Tip If bubbles are visible in the bulb area hold the electrode by the top
90. s and ChromLab Software Setting Up Remote Access Connection Setting Up Remote Access Connection You can access the NGC instrument from your home your desk or anywhere else as long as you have access to the IP address of the NGC instrument Remote access gives you full control of the NGC instrument via ChromLab Touch software as if you were physically present Remote access requires the use of a VNC viewer which employs VNC technology an open standard A variety of VNC viewers are available free on the web You can download a VNC viewer for the kind of device you want to use for example another computer a tablet or a smartphone to access the NGC instrument remotely Install the viewer before attempting to gain remote access Note Accessing the NGC instrument when you are away from your work site might require the use of VPN To access the NGC system remotely i 2 In ChromLab select the System Control tab Click Tools gt System Settings to open the System Settings dialog box In the Remote Access tab select Enable VNC service for remote screen sharing This setting persists until you change it Open the VNC viewer and enter the following settings which appear in the System Settings Remote Access dialog box Network Name system name or IP address Port No 5900 Password ngc Installation Guide 95 4 Installing ChromLab Software 96 NGC Chromatography Systems and ChromLab Software 5 Plumbing
91. s personal injury or extreme damage to the instrument Do not proceed until all stated conditions are met and clearly understood in serious personal injury or extreme damage to the instrument Do not proceed until all stated conditions are met and clearly understood A hazardous situation that could result A in minor or moderate personal injury or damage to the instrument Do not proceed until all stated conditions are met and clearly understood Electrical Shock A hazardous situation that could result Safety Precautions depending on the system in place Take care when lifting the instrument onto the lab bench or into a standard cold cabinet To reduce the risk of personal injury or damage to the instrument three or more people should perform this task i Caution The weight of the NGC system varies from moderate to heavy or moving modules No user serviceable parts are inside any component unless A WARNING Disconnect power to the NGC system before inserting removing noted in this manual Refer servicing questions to Bio Rad service personnel Installation Guide 9 1 Introduction Regulatory Information The NGC instrument has been tested and found to be in compliance with all applicable requirements of the following safety and electromagnetic compliance standards The NGC instrument is labeled with the following compliance marks Safety Compliance CE Mark m EN61010 1 Electrical Equipment for Measurement C
92. signal import module SIM to export digital signals to and import digital signals from external detectors Collect large volume fractions using multiple outlet valves while also collecting small volume fractions using the BioFrac fraction collector Automatically prepare buffers using preprogrammed buffer blending protocols Analyze purification results through 1 click peak integration determine protein concentration and calculate column performance Automate purification protocol optimization using the scouting wizard Easily locate fractions containing peaks of interest and view the protein concentration within each fraction Extend the preconfigured systems with additional valves for buffers samples and columns Organize the location of the modules to optimize separation performance based on method scale and complexity and to minimize the system swept volume Minimize errors when connecting tubing using the Point to Plumb feature in ChromLab software 8 NGC Chromatography Systems and ChromLab Software Safety Information Safety Information This section explains the different safety alerts within this document This section also provides information about the safe use and setup of the instrument Safety Alerts This document contains WARNINGS and Cautions pertaining to the installation and use of the NGC instrument Alert Icon Definition Flammable A hazardous situation that could result A Extreme Heat in seriou
93. stallation Guide 45 3 Setting Up NGC Chromatography Systems 6 Place the expansion tier on top of the already installed tier Tip If your NGC instrument is on a lab bench consider using a step stool to place the fourth tier onto the third tier Tip Although the images in this section show how to attach the fourth expansion tier to an already installed third tier the procedure for attaching a third tier to a second tier is the same These images are examples Upper expansion tier Previously attached lower tier 7 Locate the connector cable that ships with the expansion tier 46 NGC Chromatography Systems and ChromLab Software Attaching an Expansion Tier to the NGC Instrument 8 View the connector ends Each end is beveled The direction of the bevel determines the tier into which to insert the connector The end with the bevel slanting up inserts into the upper tier s connector E port m The end with the bevel slanting down inserts into the lower tier s connector port Bevel slanting down Bevel slanting up inserts inserts into the lower into the upper tier tier connector port connector port For example Fourth tier connector port okso ORO i Third tier connector port Installation Guide 47 3 Setting Up NGC Chromatography Systems 9 Firmly press the clips inward and insert the appropriate connector int
94. t are specific to the system Verify the contents against those listed in the sections that follow The accessories kits contain the tubing loops and fittings necessary to complete the setup of the shipped configuration All kits include NGC fittings kit 1 ml syringe Luer adapter Fittings tightener Touch screen and pivot arm kit Tool to attach touch screen Pump head wash inlet tubing 2 Pump head wash priming line 1 4 28 union System pump inlet tubing 2 1 ml sample loop Waste tubing 2 Injection port 20 psi backpressure regulator Tubing retainers small 1 for PEEK tubing management Tubing retainers large 2 for PTFE tubing management Column tubing 2 and fittings from inject valve to column top and from column bottom to UV detector inlet Tube 2 conductivity flow cell to 20 psi backpressure regulator Tube 4 backpressure regulator to the BioFrac fraction collector 22 NGC Chromatography Systems and ChromLab Software NGC Systems Accessories Kit NGC Scout Accessories Kit The kit for the NGC Scout system includes all the accessories in the NGC systems kit as well as Tube 2 20 psi backpressure regulator to pH valve inlet pH waste tubing pH calibration port inlet tubing Colored tubing for the buffer blending valve NGC Discover Accessories Kit The kit for the NGC Discover system includes all accessories in the NGC Scout kit as well as Pump head wash inlet tubing 2 for
95. t cable that ships with your DAC device 6 If connectors are attached to both ends of the cable remove the connector that does not insert into your device Tip Alternatively contact the manufacturer for a suitable cable 7 If necessary strip the plastic wire coating off the wires so that they are exposed 8 Determine the positive ground and trigger wires on the cable 74 NGC Chromatography Systems and ChromLab Software 10 11 2 12 Connecting Peripheral Equipment Insert the positive and ground wires into the appropriate terminals on the DAC A connector and tighten the screws taking care not to fray the wires Insert the trigger wire into terminal 2 on the DIGITAL OUT connector and tighten the screw taking care not to fray the wire DAC DIGITAL OUT DIGITALIN SIM1 SIM 2 B 0 10V A 1234GND GND GND ey L Trigger wire Ground Positive wire wire Insert the connectors into their ports on the back of the SIM Refer to the chapter System Controls in the NGC Chromatography Systems and ChromLab Software User Guide for information about how to enable signal output to the device Installation Guide 75 3 Setting Up NGC Chromatography Systems Connecting the Power and Communication Cables The power sockets and communication ports for the NGC system are located on the back of the instrument Because the back of the instrument will be very close to the back of t
96. tely 10 ml and all tubing is filled with liquid 5 Turn the priming port valve clockwise to close the port 124 NGC Chromatography Systems and ChromLab Software 6 7 Priming and Purging the System Remove the syringe from the priming port and discard the liquid into waste Repeat steps 2 6 for system pump B To purge the system pumps 1 On the NGC touch screen set the fluidic scheme to either NGC Quest or NGC Quest Plus In the fluidic scheme touch or click the Sample Inject Valve module to open its dialog box Be w D Manual Load Loop System Pump to Column D System Pump Inject Loop Installation Guide 125 5 Plumbing and Priming the NGC Chromatography Systems 4 In the fluidic scheme touch or click the System Pump module to open its dialog box 5 Inthe System Pump dialog box a b Set Flow Rate to 20 ml min Optional Set Duration to at least 2 min Clear the Enable Air Sensor checkbox Click Start System Pump F10 w e Flow Control Flow Rate 0 001 10 20 000 A ml min Mode Isocratic Gradient B o 2 4 min Duration Pressure Limits Lower o psi Upper 3650 psi _ Control Flow to avoid overpressure Enable Air Sensor gt Start 23 126 NGC Chromatography Systems and ChromLab Software Priming and Purging the System Priming and Purging the NGC Scout System Priming the
97. tems and ChromLab Software Attaching the Touch Screen 6 Insert a 4 20 x 34 hex bolt underneath the mount to secure the touch screen 7 Locate the touch screen display cable in the accessories kit 8 Firmly push the touch screen cable into the port underneath the touch screen and tighten the thumbscrews Installation Guide 27 3 Setting Up NGC Chromatography Systems Tip You can rotate the monitor if you need more room to insert the cable 9 Route the display cable through the side and rear cable troughs on the instrument 28 NGC Chromatography Systems and ChromLab Software Attaching the Touch Screen 10 Insert the touch screen cable into the touch screen connector port on the back of the instrument and tighten the thumbscrews 11 Locate the touch screen cable cover in the accessories kit This is the cover with the cable notch 12 Slide the touch screen cable cover into place to cover the cable Cover with cable notch Installation Guide 29 3 Setting Up NGC Chromatography Systems 13 Secure the cover to the back of the instrument with a 6 32 x 1 1 4 Phillips screw 30 NGC Chromatography Systems and ChromLab Software Attaching the Touch Screen Repositioning the Touch Screen Pivot Arm Assembly If you attach the touch screen to the right side of the NGC instrument you must re
98. tems and ChromLab Software Priming and Purging the System Set Flow Rate to 10 ml min Click Change Position to set the sample inject valve to Sample Pump Waste and close the dialog box Optional When an air sensor is present in the Sample Pump dialog box select Enable Air Sensor to use end of sample detection to stop the pump Sample Pump F100 2 a a8 Select Sample Inlet Port S1 Ports 7 Flow Control Flow Rate 0 01 100 10 00 ml min 5 E Set Injection Volume ml Pressure Limits Lower oE psi Upper 1450 7 psi Control Flow to avoid overpressure Inject Valve Waste Change Position gt Start V Enable Air Sensor Click Start Monitor the pump pressure and outlet flow to ensure that no air is in the lines and that the fluid is flowing properly Installation Guide 143 5 Plumbing and Priming the NGC Chromatography Systems Calibrating the Instrument The NGC instrument includes integrated pressure and temperature sensors The pressure sensors monitor the system pressure to protect the column and media from overpressure The temperature sensor in the conductivity monitor enables the system to provide real time temperature compensation for conductivity and pH measurements The measured temperature appears as a trace on the chromatogram All sensors and detectors in the NGC instrument ship precalibrated to ensure accurate monitoring ChromLab enables you to r
99. ter Important If you have anti virus software installed on your ChromLab computer configure the software to allow the following programs and services to communicate with ChromLab Bio Rad NGC ICMPv4 Bio Rad NGC Network Discovery Bio Rad NGC SQLServer Browser Bio Rad NGC SQLServer NG Bio Rad NGC Time Source Bio Rad NGC ChromLab Application If not all ports are open run the ChromLab installer again to set up the Windows firewall correctly See Firewall Inbound Rules for Bio Rad NGC Software on page 173 for a list of firewall configuration requirements 162 NGC Chromatography Systems and ChromLab Software Firmware Update Failure Firmware Update Failure If the firmware upgrade fails restart the NGC instrument The software attempts the firmware update again If the firmware update fails again 1 Shut down the NGC instrument 2 Turn off the power at the back of the instrument for 30 seconds 3 Power on the NGC instrument The firmware update procedure starts again Installation Guide 163 B Troubleshooting ChromLab Software Installation 164 NGC Chromatography Systems and ChromLab Software o C Connecting the C 96 Autosampler to NGC Systems Using the C 96 Autosampler with NGC Systems The C 96 autosampler enhances NGC chromatography systems providing automated accurate and reproducible sample injections Its easy to install accessories allow for a wide range of injection volumes from 5 pl 5
100. the sample pump Pump head wash priming line for the sample pump m 1 4 28 union Sample pump inlet tubing System pump inlet tubing 14 for inlet valves Column tubing 10 and fittings from the column switching valve Top positions to column top and from column bottom to column switching valve Bottom positions NGC Discover Pro Accessories Kit The kit for the NGC Discover Pro system includes all the accessories in the NGC Discover kit as well as Buffer inlet tubing 8 for inlet valve Tube 6 for the inlet valve Outlet valve tubing 12 for the outlet valve Tube 10 for the outlet valve Installation Guide 23 3 Setting Up NGC Chromatography Systems Attaching the Touch Screen The installation procedure for the touch screen varies depending on the location of the NGC instrument lab bench or standard cold cabinet This section explains how to attach the touch screen to the instrument in either location The communication cable for the touch screen is approximately 5 1 52 m in length A 10 or 15 3 0 or 4 57 m cable is also available Important The communication cable for the touch screen does not have a standard DVI connector even though its connector resembles one Do not attempt to connect the touch screen to the NGC system with any cable other than the one supplied with the system to avoid serious damage to the instrument NGC System Location Is on a Lab Bench Note This procedure assumes you will
101. touch screen Installation Guide 33 3 34 Setting Up NGC Chromatography Systems NGC System Location Is a Standard Cold Cabinet To connect the touch screen to the NGC system when the system is located in a cold cabinet perform the following tasks Connect the touch screen and communication cables to the NGC instrument before inserting it into the cold cabinet Place the instrument in the cold cabinet Connect the touch screen to the touch screen stand Connect the touch screen cable to the touch screen These tasks are explained in detail in the sections that follow To connect the touch screen and communication cables 1 Determine the side of the cold cabinet into which you plan to install the instrument and place a table on that side of the cabinet Locate the touch screen display cable in the accessories kit or the NGC touch screen bracket kit 788 5060 Tip Bio Rad provides a standard 5 1 52 m cable in the accessories kit and a 15 cable with the NGC touch screen bracket kit or the NGC touch screen stand 788 5040 A 10 3 0 m cable is also available NGC Chromatography Systems and ChromLab Software Attaching the Touch Screen 3 Insert the touch screen cable into the touch screen connector port on the back of the instrument and tighten the thumbscrews 4 Complete the steps in the section To connect the power and communication cables on page 77 To place the instrument in the cold ca
102. ubing to the pump wash inlet port left rear port on system pump A Pump wash 7 inlet valve on G pump A Immerse the other end of the pump A wash inlet tubing in the tube of 20 IPA Connect the pump head wash priming tubing to the outlet right rear port on system pump B Locate a 1 4 28 female to female luer adapter in the fittings kit and connect it to the pump head wash priming tubing Connect a 1 4 28 union to a second inlet tubing You will use the inlet tubing as the outlet line for system pump B Tip Set the inlet tubing with the attached union aside Keep it close by as you will attach it to the pump head wash tubing in step 11 102 NGC Chromatography Systems and ChromLab Software 10 11 12 13 Plumbing NGC Systems Attach a 30 ml syringe to the 1 4 28 female to male luer adapter Withdraw the plunger on the syringe until liquid is visible in the syringe Detach the luer adapter and syringe from the pump head wash priming tubing on pump B Locate the second inlet tubing with the 1 4 28 union that you set aside in step 7 Connect the union to the pump head wash priming tubing on system pump B Immerse the other end of the inlet tubing in the tube of 20 IPA To plumb the sample pump Perform the steps in the section To plumb and prime the system pump wash lines on page 102 with the following exception gt Attach the inlet tubing to the left rear port on the sample pump and the pump he
103. uses three sizes of tubing Use the information in Table 7 to select the appropriate tubing Table 7 NGC tubing sizes Use Tubing Dimensions Tubing Fittings Vol cm Material Prepump 1 8 OD x 0 062 ID Clear Super flangeless 20 ul 3 2 mm OD x 1 6 mm ID PTFE for 1 8 3 2 mm OD tubing Postpump 1 16 OD x 0 020 ID Orange Super flangeless 2 ul with F10 1 6 mm OD x 0 51 mm ID PEEK for 1 16 1 6 mm pump head OD tubing Postpump 1 16 OD x 0 030 ID Green Super flangeless 4 5 ul with F100 1 6 mm OD x 0 76 mm ID PEEK for 1 16 1 6 mm pump head OD tubing Note When plumbing the system keep tubing lengths to a minimum Also all fittings should be finger tight Do not overtighten or you risk damaging the connection Bio Rad offers tubing clips of different sizes in the accessories kit For outlet tubing longer than 6 attach a tubing clip in close proximity to the fitting to keep tubing in place in the event that it detaches from the fitting Installation Guide 99 5 100 Plumbing and Priming the NGC Chromatography Systems Plumbing All NGC Systems Table 8 lists the tasks for plumbing all NGC systems The tasks are explained in detail in the sections that follow Table 8 Plumbing tasks for all NGC systems Task 1 Inlet tubing to the pump head wash port on system pump A 2 Pump head wash priming line to the pump head wash port on system pump B 3 1 4 28 union to the priming line on system pump B 4 Pum
104. ver system you prime and purge the following modules Buffer blending valve module Buffer inlet valves modules Sample pump module Sample inlet valve module NGC Discover Pro series Priming the NGC Discover System Note This section assumes that you have not introduced buffer into the lines If you have buffer in the lines and are purging the lines of small air bubbles proceed to Purging the NGC Discover System on page 138 Important The instrument s buffer lines cannot be primed when the system is under pressure If your system does not have a buffer blending valve turn the inject valve to Waste to release the system pressure before opening the priming port To prime the buffer blending valve 1 Immerse the inlet tubing for ports Q4 Q1 in their respective buffer containers See To plumb the buffer blending valve on page 115 for more information 2 On the ChromLab computer or the touch screen start ChromLab in Manual mode 3 Set the fluidic scheme to NGC Discover Installation Guide 131 5 132 Plumbing and Priming the NGC Chromatography Systems 4 In the fluidic scheme touch or click the System Pump module to open its dialog box 8 3 8 3 I wuja In the System Pump dialog box select Priming on the Mode dropdown list Note When in Priming mode the sample inject valve switches the system pump flow to waste W2 After the process is completed the sample inject valve switches the
105. ystem pumps A and B are primed and purged when the buffer blending valve is primed Prime and purge Q1 Q4 on the buffer blending valve Tip The inlet ports on system pumps A and B are primed and purged when the buffer blending valve is primed Prime and purge the buffer inlet valves A and B Prime and purge the sample pump Installation Guide 123 5 Plumbing and Priming the NGC Chromatography Systems Priming and Purging the NGC Quest System columns are offline The priming process occurs at a high flow rate and can Caution Before priming or purging the system ensure that any attached significantly harm or destroy attached columns Priming and purging the NGC Quest system requires access to ChromLab You can perform the steps on the NGC touch screen Important The instrument s buffer lines cannot be primed when the system is under pressure Before opening the priming port turn the inject valve to Waste to release the system pressure To prime the system pumps 1 Immerse the inlet tubing for both system pumps A and B in a container of buffer or HPLC grade filtered degassed or other high quality water 2 Insert a 30 ml syringe into the priming port on system pump A 3 Turn the priming port valve counter clockwise one full turn to open the seal Outlet Priming port Inlet 4 Slowly withdraw the plunger to withdraw liquid from the port until air no longer enters the syringe approxima
106. ystems and ChromLab Software Establishing the Connection Between ChromLab Software and the NGC Systems 4 Press the soft power button on the side of the NGC instrument to power on the system Soft power switch Peripheral connector ports USB ports Installation Guide 85 4 Installing ChromLab Software Setting the System Name The tasks in this section are performed on both the ChromLab computer and the NGC instrument s touch screen To set the NGC system name On the NGC system 1 When ChromLab Touch loads on the touch screen you are prompted to provide a name for the NGC instrument By default the name is NGC This is the name that appears on the ChromLab Home screen when it connects to the NGC system This is also the name that appears on the run reports to identify the system on which the run was generated You can use any alphanumeric or special characters However you are limited to a maximum of ten characters Welcome to your new NGC Chromatography System Name your system NGC 86 NGC Chromatography Systems and ChromLab Software Establishing the Connection Between ChromLab Software and the NGC Systems Touch or click OK The ChromLab connection information dialog box appears This dialog box explains how to connect the ChromLab computer to the NGC instrument Follow the instructions provided on the connection dialog box Note Write down either the Network Name or the I

NGC Installation Guide - Bio-Rad

Contents

Download Pdf Manuals

Related Search

Related Contents