Harlan 4 Drive User Manual
Contents
1. Add the other drives and test their travel If there is any binding remove the drive and check that a separate piece of 010 tubing can be slid into the drive tube assembly up to the drive plate If excess Cyanocrylate has been used it may have wicked between the tubes and bonded them together If so replace the tube assembly with another If the outer tube does not pull out of the drive plate fairly easily soaking the tip of the drive plate in water for a few hours should soften the Harlan 4 Drive User Manual Page 8 Cyanocrylate Try to keep the screw out of water 10 Make up the tetrodes according to your standard procedure Back off the drives until the heads of the drive screws are just below the ends of the guide pins This should keep the outer tubes in the body holes Insert the tetrodes into the inner tubes and slide them through until they are just flush with the tip guide tubes Bond them with Cyanocrylate adhesive to the tops of the inner tubes 11 Cut the tetrodes at 12 mm above the ends of the inner tubes Separate about 6 mm of the wires and feed them into their respective recording channel holes on the connector board The end of each wire should extend about 2 mm above the top of the board Insert a cactus needle into each hole from the top of the board and push firmly to ensure contact of the wire with the circuit trace Break off the excess cactus needle Break off excess tetrode wire with forceps 12 After all th2. f Neuralynx High Density Electrophysiology VX Systems Harlan 4 Drive User Manual Microdrive used for manually driving tetrodes for electrophysiology recordings Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 Fax 866 585 1743 www Neuralynx com Revision 1 1 support Neuralynx com 7 2 2013 Table of Contents l D m OVE EW ta 3 2 Harlan 4 Drive Overview adas 3 O E was a a ates sagt deca a E a aae A E a A TE R i 4 Ai Dive Specifications nassen a aa T E E A E ETEA 5 E Diye Pp o 6 6 MNicr drive Implantar 10 T R cy lne th Microdrive At lso 11 8 Appendix B Microdrive Parts Identifier cncioncnioninicin tiara 15 List of Figures and Tables Figure 5 1 Initial Telescoping Tube AssembDiVocastn dit ate 7 Figure 5 2 Final Telescoping Tube Assembly ii vtecesageds 7 Figure 5 3 Drive Assembly ni A i 8 Figure 10 1 Microdrive Parts Identifier aci ia a adds 15 Figure 10 2 Neuralynx s EIB 16 and ElBaradei dais 15 Revision 1 1 Harlan 4 Drive User Manual 7 2 2013 Page 2 1 Document Overview The purpose of this manual is to outline assembly loading and general implantation of the Harlan 4 microdrive It also describes the appropriate preparation and recycling procedures surrounding the experiment Step by step instructions are outlined and additional products like the Drive Assembly Fixture are recommended to further facilitate this process 2 Harlan 4 Drive Overview The
3. tip hole The ID needs to be at least 031 The following steps will ensure properly working drives Careful attention paid to tube lengths and assembly geometry will make the job of installing the drives easier 1 Loosen the cover screws and remove the cover of the microdrive Remove the body screws from the cone and remove the body Store the small screws in a secure place Remove all of the drives 2 Cut one piece of each size of tubing for each drive to be assembled Use a new single or double edged razor blade to avoid crushing the tubing Cut the following lengths a 12 5 mm of 0056 OD b 15 mm of 010 OD c 2mmof 012 OD d 10 5 mm of 0196 OD Revision 1 1 Harlan 4 Drive User Manual 7 2 2013 Page 6 The 012 tube is used as a spacer between the 0056 and 0196 tubes The thicker wall helps center the small tube The 010 tubing can be used instead if a small quantity of the 012 tubing cannot be obtained ask for a sample one foot piece which will make 150 assemblies 3 Slide the 2 mm length 012 tube over the 0056 tube so that Y2 mm of the small tube extends past the end of the 012 tube Bond these together with a thin or gap filling Cyanocrylate adhesive such as Zap or Zap A Gap Use the point of a straight pin needle or a flat tip oiler black to apply the tiniest drop of adhesive and then use some accelerator if the bond does not set quickly Be very careful not to get any a
4. BLE TIP INTERMEDIATE TUBE GUIDE TUBE Figure 8 1 Microdrive Parts Identifier The Harlan 4 Drive is compatible with Neuralynx s EIB 16 on left or EIB 18 on right Figure 8 2 Neuralynx s EIB 16 and EIB 18 Revision 1 1 Harlan 4 Drive User Manual 7 2 2013 Page 15
5. Harlan 4 Drive is a 4 tetrode microdrive that allows for recordings from a single brain structure or from two or more structures that fall on one line that extends from the skull surface into the depths of the brain For initial drive preparation assemblies of telescoping polyamide tubes must be built and attached to the microdrive The Drive Assembly Fixture aids with loading and the tube assemblies are inserted into the drive plate and bonded with an adhesive The tetrodes are fed through the guide tubes and the assembly is inserted with drive screws into the cone The tetrodes are gold plated the cone components are assembled and the drive is ready for experimentation A general implantation outline is provided but there will be variations according to particular animals and objectives of the research to be accomplished After experimentation recycling guidelines describe how the drive can be reused on other animals with a new replaceable tip and new polyamide tube assemblies Ray Harlan is the maker of this drive and can be contacted at Specialty Machining 15 Happy Hollow Road Wayland MA 01778 Phone and FAX 508 358 4013 rbharlan comcast net Revision 1 1 Harlan 4 Drive User Manual 7 2 2013 Page 3 3 Glossary Inner Diameter ID the inner diameter of the polyamide tubing Outer Diameter OD the outer diameter of the polyamide tubing EIB Electrode Interface Board Revision 1 1 Harlan 4 Drive User Manual 7 2 2013
6. Implantation The following outline is meant to be a general guide to implanting the microdrive There will be variations according to particular animals and objectives of the research to be accomplished Revision 1 1 7 2 2013 1 2 10 11 12 13 14 15 Shave head area around location of incision Secure anaesthetized animal in stereotactic fixture Make incision and hold skin apart with appropriate clamps Scrape away tissue over the skull Attenuate any bleeding Mark the stereotactic reference point on the skull e g Bregma with fine point permanent marker Insert pointer in stereotactic holder and move over reference point Record axes coordinates Calculate burr hole coordinates Move stereotactic holder to the hole and mark location with permanent marker Remove the pointer Mark locations for anchor screw holes and ground hole Drill screw holes with small dental burr being very careful not to penetrate the dura For the ground hole the dura is pierced Insert anchor screws suggested screws 3 32 00 90 or 0 80 hex head screws The bottoms of the heads should clear the skull by 5 to 1 5 mm Drill the burr holes with a core drill approx 1 3 mm dia Do not penetrate the dura Carefully scrape away the dura in the burr holes Attach the microdrive holder to the Stereotactic Adapter Attach the microdrive to the holder Move the guide tube over the stereotactic reference po
7. Page 4 4 Drive Specifications Travel for each drive Travel for each revolution of the drive screw Height of microdrive Diameter of microdrive Weight Without Cover With Cover Revision 1 1 Harlan 4 Drive User Manual 7 2 2013 7 9 mm 160 micrometers 28 4 mm 18 9 mm 2 1 grams 2 6 grams Page 5 5 Drive Preparation Before the microdrive can be used assemblies of telescoping polyamide tubes must be built and attached to the microdrive These tubes provide the rigidity needed to precisely deliver the tetrode tips to the intended locations For each drive one tube is attached to a guide tube in the tip Three other tubes are telescoped together and attached to the drive plate After the drive is installed in the microdrive the tetrode is inserted in the innermost tube and bonded to it Four or more sizes of polyamide tubing are used in the microdrive The exact sizes and wall thickness may not always be available from tubing vendors There is some flexibility in choosing sizes it is best to try to get wall thickness specified here and adjust diameters as needed Try to provide 001 003 in diametral clearance between telescoping pairs of tubes The recommended sizes from smallest to largest are 0056 OD 0035 ID 001 wall 10 OD 008 ID 001 wall 012 OD 0065 ID 0028 wall 0196 OD 0135 ID 0030 wall Guide tubes for the tip should be approximately 046 OD to fit the
8. cycled for further use on other animals Because of the nature of the bonding technique the polyamide tubes must be discarded If care is taken when removing the tube assemblies from the drive plates the drives will be fully reusable The outline below suggests a sequence to be followed 1 Revision 1 1 7 2 2013 Remove the microdrive from the animal If dental acrylic has been kept away from the attachment screws then only chipping it away from around the ground wire will be required Carefully work the adhesive off with a sharp knife Avoid cutting into any part of the cone If a gel type adhesive was used to bond the guide tube to the body carefully cut it away avoiding cutting the guide tube The tube then can be removed by holding with pliers and twisting the tube until any remaining bond is broken When the tube is pulled out of the body it will take with it the intermediate polyamide tubes If there is any cement on the cone that isn t easily scraped away it can be softened by soaking the cone Harlan 4 Drive User Manual Page 11 in a shallow dish of acetone for an hour This makes the cement somewhat rubbery Remove the drives from the body Cut the tetrode wires with scissors and the drives should be readily removable Remove the tube assemblies from the drive plates Cut the top of the tubes and any glue fillet off flush with the top of the drive plate with a sharp razor blade If care was taken to minimize the amou
9. dhesive inside the smaller tube The thinner adhesive seems to do a cleaner job Cut cured adhesive off the applicator frequently 5 mm Figure 5 1 Initial Telescoping Tube Assembly 4 Insert this assembly 0056 tube first into the 0196 tube until Y mm of the 012 tube extends past the end of the large tube Bond this assembly together as in Step 4 again being careful not to get adhesive in the small tube Note that the 0056 tube extends about 1 mm past the bottom end of the 0196 tube This helps when inserting it into the 010 tube in the drive The top of the assembly should look like a stepped cone with both 012 and 0056 tubes extending equal amounts from their next larger tubes nae Figure 5 2 Final Telescoping Tube Assembly 5 Insert the drive screw into the Drive Assembly Fixture hole furthest from the top end of the fixture Push it in until the retaining nut seats against the fixture Insert the tube assembly into a drive plate and assembly fixture until the 0056 tube at the bonded end extends 2mm past the top surface of the plate Bond the assembly to the plate with Cyanocrylate adhesive If a gel type adhesive is used it is possible to just put a tiny fillet between the tube and drive plate and not have it wick down the hole This will facilitate removing the tube to recycle the microdrive Accelerator must be applied to achieve curing A small bottle that has a Luer top that can take a
10. e wires have been connected dress them away from the guide pins so there is no chance of binding or snagging as the drives are advanced 13 Cut a piece of insulated tungsten ground wire approximately 150 mm long Strip the insulation off both ends Feed the wire into the ground wire hole in the body from below This hole will be located between two drives near the outer periphery of the body and near the rear of the drive Slide the wire through until the end can be inserted into the ground hole on the connector board Secure it with a cactus needle and break off excess needle 14 Feed the ground wire into the ground hole in the lower part of the cone Reassemble the cone to the body aligning the ground holes in the body and cone one above the other Pull the wire through leaving sufficient slack so that the wire won t snag on a drive Solder a lug fashioned from copper tin foil to make good contact with the ground screw The excess wire can be wrapped around the lower cone and taped in place temporarily with small bits of Scotch tape 15 Gold plate the tetrodes using your standard protocol 16 Retract the tetrodes by backing out the drive screws until they are just free of the body Seal the intermediate tubes by touching the end of the guide tubes to a light mineral oil Blot off excess oil with an absorbent wiper The microdrive now is ready for implantation Revision 1 1 Harlan 4 Drive User Manual 7 2 2013 Page 9 6 Microdrive
11. eassembled with new polyamide tube assemblies Revision 1 1 7 2 2013 Harlan 4 Drive User Manual Page 12 Appendix A Potential Vendor List Polyimide tubing PD Wire and Cable Trenton GA 800 241 1075 HVT Technologies Trenton GA 706 657 7700 MicroLumen Tampa FL 813 886 1200 Flat tip oilers S LaRose Inc Part No OL 350 extra small black Greensboro NC 888 752 7673 Dental acrylic Patterson Dental Hygenic Repair Resin veined Wilmington MA 800 842 5355 Small screws J I Morris Company Southbridge MA 508 764 4394 Tungsten ground wire A M Systems Inc Catalog No 7960 Carlsborg WA 003 wire 0045 dia insulated 800 426 1306 Cyanocrylate adhesives Any local hobby shop Pacer Zap Zap A Gap Slo Zap and Zap Gel Also house brands and accelerator Revision 1 1 Harlan 4 Drive User Manual 7 2 2013 Page 13 Tower Hobbies Champaign Il 800 637 4989 Home Depot Super Glue Gel and Future Glue Gel Dispensing bottle for accelerator Contact East Precision Dispenser Part No 121 723 800 225 5370 Needles Part No 121 755 009 ID Small Parts Squeeze Bottles Part No USQB 02 800 220 4242 Needles U NE 301PL or U NE 271PL Revision 1 1 Harlan 4 Drive User Manual 7 2 2013 Page 14 8 Appendix B Microdrive Parts Identifier CONNECTOR BOARD BODY ORIVE PLATE GUIDE RODS POLYIMIDE TUBE ASSY ATTACHMENT SCREWS DRIVE SCREV 00 90 X 3 32 HEX HEAD CONE REPLACEA
12. hypodermic needle is ideal for applying just a tiny drop onto the glue Try not to touch the needle to the glue it can get Revision 1 1 Harlan 4 Drive User Manual 7 2 2013 Page 7 Revision 1 1 7 2 2013 clogged Use a 26 gauge or finer needle hold the bottle inverted and just let gravity form a drop on the tip of the needle 2 mm S Figure 5 3 Drive Assembly Cut the tip guide tube to 5 mm long Insert it into the tip of the body It should extend exactly 2 mm beyond the end of the tip Bond it in place with a small dab of gel type Cyanocrylate adhesive Insert the intermediate tubes 010 OD into the inner holes in the body Push them through until they exit the tip When all tubes are in place push them further until all are about 1 2 mm above the top surface of the body Bond them to the guide tube with low viscosity Cyanocrylate adhesive Then cut the tubes off flush with the guide tubes Place a drive over the guide pins so the pins enter the holes in the drive plate Slide the drive down until the smallest tube almost touches the intermediate 010 tube Guide the small tube into the 010 tube as the drive is moved further downward The drive screw now can be engaged in the body threads to move the drive Guide the outer tube over the 010 tube as the drive is advanced When all the tubes are telescoped test the freedom of the assembly by advancing the drive over the full travel range 7 9 mm
13. int and lower the microdrive until the guide tube clears the skull by less than 1 mm Adjust the A P and Lateral coordinates until the guide tube is well centered over the Harlan 4 Drive User Manual Page 10 16 17 18 19 20 21 22 reference mark Record the coordinates Calculate the new coordinates for the guide tube and move the stereotactic fixture to them Unwrap the ground wire from the lower cone and attach it to the proper screw Take up excess slack in the wire by wrapping it back and forth around a pair of adjacent screws Lower the microdrive until the guide tube touches the brain surface Fill around the tip with Vaseline to seal it Keep the Vaseline confined to the tip do not allow it to smear onto the skull area that is to be bonded with dental acrylic Mix small batches of dental acrylic and fill around screws and the replaceable tip slowly building up the mass with sequential batches Cover the screws ground wire and build up around the lower portion of the cone The final shape of the cement mass should be smoothly formed around the tip and feathered out onto the skull to facilitate comfort for the animal Suture the incision bringing the skin over some of the dental acrylic Do not pull too tight Release the microdrive and remove the holder Remove the animal from the stereotactic fixture 7 Recycling the Microdrive After the microdrive has been removed from the animal it can be re
14. nt of Cyanocrylate used to attach the assembly to the plate the tube should pull out fairly easily If it resists use a 022 dia drill in a pin vise to clean out the tube remaining in the hole Be very careful not to twist the drill sideways in the hole and put excessive strain on the drive plate Do not apply any pressure to the drive screw or it may bend Do not attempt to use any debonder or other solvents to soften the adhesive they may produce stress cracks in the plastic drive plate Remove tetrode wires from the connector board It may be helpful to remove the board from the microdrive make note of the orientation of the board on the microdrive Some cactus needles will protrude through the board by 3 or more millimeters It may be best to cut all of the needles off with diagonal pliers so that about 1 mm protrudes Use a flat bladed screwdriver to pop the cactus needles back up through the plate Try to push on them straight along their axes to avoid bending and breaking If some do break use a flat ended piece of 015 music wire available at hobby shops held in a pin vise to push on the cactus needle The wire should extend only about Y2 mm from the pin vise for the first push It may have to be extended more to push the loosened cactus needle out of the board After all of the needles and bits of tetrode wire have been removed from the board replace it on the microdrive At this point the microdrive should be ready to be r
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