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PeakNet IA Software User`s Guide

1. Caution There are differences between the operation of the Dionex Data System under Windows 98 and Windows NT Windows 2000 For details see the following topic in the chapter In stallation The Dionex Data System under Windows 98 and Windows NT 2000 Network The network capability of the Dionex Data System allows operation of the data system within local and global networks also referred to as LAN local area network or WAN wide area network The Dionex Data System can not only be operated locally in a single user in stallation but also within a network This includes data transfer and remote op eration via ISDN Dionex Data System stations can be linked around the world wide area network To enjoy the advantages of network operation including centralized data stor age backup and administration shared access to methods and world wide availability safe and fast data exchange is essential Even state of the art networks sometimes have difficulty coping with the enor mous amount of data Therefore client server systems offer decisive advan tages by specifically selecting transferred data Client Server System From a decentralized PC client sub programs are started on a central com puter gt Server or Chromatography Server via gt RPC commands Remote Hardware and Software Chromatography Components 9 Procedure Calls The server executes the actual work searching for data in a database running an
2. After PeakNet IA is installed start the gt Server Configuration program and as sign each DX 80 to a timebase Refer to nstalling the PeakNet IA System Document No 031717 for details How to 15 Enabling and Disabling System Wellness Functions The Properties dialog box for the DX 80 lon Analyzer includes two tab pages Calibration and Diagnostics that allow you to enable or disable individual cali bration and or diagnostic functions By default all DX 80 calibration and diag nostic functions are enabled Follow the steps below to disable calibration and or diagnostic functions for the DX 80 or to enable functions that are currently disabled 1 Start the Server Configuration program 2 Under the gt Timebase select the DX 80 for which you want to disable or enable the calibration and or diagnostic functions 3 Select Properties from the Edit or context menu and select the Calibration or Diagnostics tab 4 Enable or disable individual calibration or diagnostics functions by clicking the Activate box next to each function in the list To enable or disable all calibration or diagnostics functions right click and select the Activate All or Deactivate All command from the context menu 76 How to Actions in the Control Panel See the following topics for details on how to load a AJ Control Panel and how to modify the timebase assignment n Loading a Control Panel n Connecting a Control Panel with a Timebase
3. Samples and Sequences 54 e Perform a gt Ready Check e Start the analysis process by clicking the Start button Processing the Automatic Batch As soon as the online batch is started all samples of the sequence with the status single are analyzed successively If a sequence contains a sufficient number of samples sample processing around the clock is possible Instead of including all samples in one sequence they can be distributed in several sequences Accordingly more sequences a maximum of 16 are then entered in the batch dialog box This list can be considered a batch it is also referred to as online sample batch or online batch The order of the sequences determines the order of process ing when starting the batch process samples 1 to n of the first sequence are analyzed then samples 1 to n of the second sequence and so on Saving the Results The results of the online batch are saved with the individual samples Sample Evaluation In spite of largely automated work processes and intelligent pre settings it is the responsibility of each user to determine the framework conditions of sample evaluation Result Output The result of sample processing can be represented in graphics and tables either on the screen or as printed output The Dionex Data System provides method windows for generating this output For a list and detailed description of available method windows see AJ Data Reprocessing Validati
4. See the following topic for details on how data acquisition can be simulated in a control panel n Using Recording Demo Data Loading a Control Panel Before processing a new unknown sample or beginning operation the DX 80 lon Analyzer control panel gt Panels must be loaded either manually or auto matically Manually The DX 80 control panel is named DIONEX_DX80_SYSTEM PAN Search for the file directly in the gt Browser in the Panels directory of the local gt Data source Select the control panel and double click the name to open it Each window must be connected with a gt Jimebase When opening the win dow the Dionex Data System automatically establishes a connection to the most recently used timebase This is usually the timebase that was assigned during installation Naturally each window can be connected with any other timebase see How to al Connecting a Control Panel with a Timebase Automatically When starting the Dionex Data System the most recent Workspace is loaded If this view contains a control panel it will also be loaded If it is not possible to load a workspace for example because no such view was arranged by the user the control panel must be opened manually How to TT Connecting a Control Panel with a Timebase When loading the DX 80 lon Analyzer control panel gt Panel it is automati cally connected with the previously stored gt Timebase This timebase assign me
5. The Protocol text is included in the Audit Trail and is thus directly linked with the corresponding sample The Protocol text can thus be used for commenting individual samples Quantification Method QNT Method A Quantification Method also known as working instructions includes all parameters that are used for evaluation of a chromatogram Thus the QNT Method serves as the basis of calculation for evaluating a sample QNT Methods cannot be edited Query The Dionex Data System allows the user to efficiently search directories se quences samples or comment lines for specific names or character strings e Inthe gt Browser choose the Query command via the right mouse button or select New Query using Wizard on the File menu A wizard opens to facilitate entering the desired conditions e On the first page define whether the query is performed in the currently open gt Datasource or in a different one In addition define whether to find certain sequence properties and or sample properties and or other result properties e After clicking Finish you can enter additional conditions to limit the query Based on the entered information the Dionex Data System generates an SQL statement that can start a query within all gt ODBC capable databases For more details see How to Al Actions in the Browser Performing a Query Glossary A 25 The result of a query can be saved as a new sequence via the Save as com
6. bration data values and their corresponding dates The dates indicate when the values were uploaded from the DX 80 to the Dionex Data Sys tem e To download calibration data to the DX 80 select Current Previous or Factory from the list to the right of the Download button Then select Download Performing Device Calibrations The Wellness control panel displays calibration data and provides command buttons for calibrating the DX 80 lon Analyzer After a calibration is performed the DX 80 uploads the new calibration data to the Dionex Data System B Note You may need to complete preliminary setup steps before the calibration procedure begins Before selecting a calibra tion command from the control panel see the topics below for details about the particular calibration task you plan to perform 102 How to Calibration Procedures ml Pressure Transducer Offset Al Conductivity Cell Calibrating the Pressure Transducer Offset When to Calibrate As required 1 Turn off the pump flow 2 Open the waste valve on the front of the pump head 3 Open the System Wellness control panel for the DX 80 lon Analyzer see How to l Opening a Wellness Control Panel 4 Click the calibrate offset command button under pressure transducer The pump calibrates the offset and uploads the new value to the Dionex Data System The Dionex Data System stores this new offset as the current value 5 Close the waste valve 6 C
7. 2 DIONEX PeakNet IA Software User s Guide 2002 Dionex Corporation Document No 031736 Revision 02 April 2002 Now sold under the Thermo Scientific brand Thermo SCIENTIFIC 2002 by Dionex Corporation All rights reserved worldwide Printed in the United States of America This publication is protected by federal copyright law No part of this publication may be copied or distributed transmitted transcribed stored in a retrieval system or transmitted into any human or computer language in any form or by any means electronic mechanical magnetic manual or otherwise or disclosed to third parties without the express written permission of Dionex Corporation 1228 Titan Way Sunnyvale California 94088 3603 U S A DISCLAIMER OF WARRANTY AND LIMITED WARRANTY THIS PUBLICATION IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND DIONEX CORPORATION DOES NOT WARRANT GUARANTEE OR MAKE ANY EXPRESS OR IMPLIED REPRESENTATIONS REGARDING THE USE OR THE RESULTS OF THE USE OF THIS PUBLICATION IN TERMS OF CORRECTNESS ACCURACY RELIABILITY CURRENTNESS OR OTHERWISE FURTHER DIONEX CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION AND TO MAKE CHANGES FROM TIME TO TIME IN THE CONTENT HEREINOF WITHOUT OBLIGATION OF DIONEX CORPORATION TO NOTIFY ANY PERSON OR ORGANIZATION OF SUCH REVISION OR CHANGES TRADEMARKS PeakNet Self Regenerating Suppressor and SRS are registered trademarks of Dionex Corporatio
8. If the operators gt lt gt or lt are used for text variables the alphabetical or der is considered e g A lt B D Tip All SQL time queries containing a relative reference operator during the previous in the Edit Condition window are re calculated for each query How to 93 Wildcards placeholders represent character strings The following wildcards are valid for text variables with the operator is not like Wildcard KC _ underscore Significance Any character string with OU or more characters Any single character A single character within a specified range e g a f Any character with the exception of the specified range As soon as the search conditions have been completed you click the Apply button to start the query Further conditions can be entered on the Result Restrictions page of the Wizard or in the Properties dialog box via the Edit Conditions page of the Result Restrictions DO Tip Depending on the number of samples resulting from the SQL query the execution of the result restriction may take some time After about 3 seconds a window will indicate the status of the result restriction in of the samples to test The user can cancel the query Only the samples of the SQL query that have successfully passed the result restrictions so far will be listed as query results 94 How to Examples Wizard The following SQL query searches for all samples e with
9. Without requiring additional input these predefined report sheets are available for various purposes Peak Summary To generate a Al Peak Summary use the worksheet by this name in the win dow section Report The required steps are described under How to H Displaying a Report In contrast to a normal gt Report which lists the data of various peaks in one sample the Peak Summary represents data of a specific peak from several samples generally a sequence A line therefore contains not only the informa How to 111 tion on one peak but information on one specific peak in a sample The user decides which peak to represent by selecting the peak in the chromatogram The default Summary report contains several columns In addition to the sam ple name and the retention time it also includes columns for Area Height Amount Types and Plates As soon as a user selects a peak in the chroma togram the values of this peak are included in the Summary report If the se lected peak is not contained in a sample this line remains empty n a not available Below the sample list are lines for the Average and Relative Standard Devia tion The average value of a column is calculated and displayed The relative standard deviation from this value is indicated in percent The user can modify the default Summary report at any time However this is not advisable if you need another report create an additional Peak Summary rep
10. mand QNT Files and PGM Files are copied raw data is copied optionally File names occurring more than once receive a consecutive number The following symbol indicates the query sequence fra QNT Method See gt Quantification Method QNT Method Raw Data Raw data refers to all analog and digital data points that are stored digitally on a PC Therefore raw data exists only for signals that were selected by the user prior to data acquisition For further details see the following topics in the Data chapter Raw Data Storage Sl Raw Data Compression Ready Check The gt Ready Check allows the user to verify that the connected instruments are ready to operate i e to process a sample batch The following items are checked e Are all instruments switched on Are all instruments connected correctly e Are all required files available e ls there sufficient storage capacity You can perform a ready check via the Ready Check button in the Batch dialog box Also a ready check is automatically performed for each online command If there is a problem an error message or warning will be displayed A 26 Glossary Reconnect Use the Reconnect command to check the connection between the PC and the timebase or between the instrument and the data system If there is no connection the gt Connect command will be issued automatically Replot from Beginning Enable the Replot from beginning option to automatically r
11. Datasource setup are initiated using the Data sources command on the File menu e Open the gt Browser and select the Datasources command on the File menu The datasource manager lists all gt Datasources that can be accessed by the gt Client 82 How to iP Tip As a minimum the default datasource lt PC NAME _LOCAL gt created during the Dionex Data System in Stallation should be visible here If a local station is not inte grated on a network the name entered in the operating sys tem under Control Panel Network Identification is used instead of the computer name If no identification was en tered the datasource is named DEFAULT_LOCAL For information about the possible actions at this point see the following topics Connecting a Database Connect nl Disconnecting a Database Disconnect n Creating a New Database New Al Opening the ODBC Manager ODBC Moar Connecting a Database In order to access data in an existing gt Database there must be a connection between the gt Client and the database e Open the gt Browser and select the Datasources command e Select the Connect button in the datasource manager to establish a con nection to a datasource e Select the type of the underlying datasource It is possible to connect to Dionex Data System databases Native Dionex Data System Datasource default Microsoft Access databases mdb contain ers and to SQL and Oracle database
12. How to Actions in the Server Configuration Program Selecting the Copy Protection Location and Entering the Key Code LOG The LOG command allows the user to document the values of variables in the Audit Trail at any time This option is especially useful for logging information that is not periodically retrieved The function is available by selecting Command on the Control menu Moduleware The instrument control firmware installed in each DX 80 lon Analyzer The DX 80 Moduleware sends status information and data to the Dionex Data System and receives control parameters from the Dionex Data System Monitor Only Mode If a connection is established to the local server via a control panel you can control the instruments connected to a timebase via the controls on the control panel control mode The purpose of monitor only mode is to prevent multiple users from simultaneously controlling a timebase For example assume that user A has control of the local timebase If user B accesses the same timebase this user is automatically assigned monitor only status This means that user B can view all current values and states in the control panel but cannot change any of them Changing from Monitor Mode to Control Mode To change between the two modes activate or deactivate the Monitor Only command on the Control menu Multi Tasking Multitasking refers to the simultaneous operation of several programs on a PC Allocati
13. The maximum of stored values corresponds to the number of values generated per second Sensitivity Signal Parameter The Sensitivity parameter adjusts the data system to the sensitivity of the de tector A 30 Glossary Sequence Sequence Table Sample List A sequence combines samples that belong together due to their origin or proc essing The names of all samples that belong to one sequence are entered in the sample list also called sequence table A sequence is thus a container for various samples To create a sequence use the nl Sequence Wizard Caution Do not use special characters e g the umlaut for new di rectory names or sequences as this may cause problems on Novell networks The sequence table also defines how to process a sample Sequences are usually started from a control gt Panel However they can be started directly in the gt Browser as well provided the sequence has already been assigned to the appropriate gt Timebase Either press Start on the gt Batch menu or the cor responding button of the online gt Toolbar All data collected in connection with creating and processing a sequence in cluding raw data and protocol data recorded during the analysis are saved in the sequence directory of a datasource or in the underlying database Of course this includes the raw and protocol data recorded during the analysis Similar to datasources sequences can be locked When a sequence is locked
14. data and results can be read but they cannot be modified or extended For details on locking datasources directories and sequences see Data Datasource Sequence Data Data that serve to characterize a gt Sequence instead of a single sample see gt Sample Data are referred to as Sequence data This includes for example the date of the last change or the corresponding timebase As with the sample data this information is stored in a database Chromatography Server PCs connected to the components of an IC or HPLC system via a serial inter face can be used as chromatography servers A chromatography server is automatically installed on the PC during the Dionex Data System installation The chromatography server controls the data ex Glossary A 31 change between the system and the PC Upon starting each server is capable of serving up to 6 controlled or 16 non controlled systems timebases The server receives the commands entered by a gt Client PC in a gt Panel and exe cutes them at the specified time by transmitting them to the corresponding device driver of the system In the opposite direction the server also assumes this buffer function Thus the raw data of each system are stored at the loca tion specified by the client and all system relevant data are forwarded to the client Starting loading the server can be performed manually or automatically Use the gt Server Monitor Program to select the req
15. diagnostic commands are available from the Wellness control panel System Wellness Features The System Wellness control panel which allows easy access to diagnostic and calibration commands and data Download of current previous or factory calibration data Pressure transducer calibration Conductivity cell calibration Pump and cell diagnostics For instructions on setting up and using System Wellness features see How to Fl System Wellness Data Reprocessing 59 Data Reprocessing Definition Data Reprocessing Data reprocessing is the presentation of raw data under various aspects Each operation is displayed in a separate window Each partial method is in tended for one specific task and has its own window arrangement and menu structure The Dionex Data System currently features the following partial method which is described in a separate topic n Integration Integration 60 Integration Overview Integration The chromatographic process of converting peak areas below peaks in amount or concentration values is referred to as integration The integration is per formed on the basis of the calibration It supplies a calibration curve and a cali bration coefficient Inserting the calibration coefficients in the formula for amount calculation sup plies the amount concentration in a single peak area The data system auto matically performs this process for all peaks it finds Aa J Calibration
16. lt Operator gt lt gt lt gt and lt gt lt Value gt Fixed point number The dimension of the lt Value gt corresponds to the sig nal dimension Parameters that are combined with DELTA have the dimen sion signal dimension sec If several parameters are to be linked this can be realized via mathematical or logical AND OR NOT XOR operators Round brackets show combinations and hierarchy Glossary A 39 Logical links of entire conditions are also possible Adding Condition is op tional Caution A trigger is executed whenever the condition changes from false to true flank triggering If a condition is true at the beginning the trigger cannot be changed from false to true This applies as long as the condition is set to false again eg Manually or via another trigger We therefore recom mend determining specific start conditions at the beginning of a trigger command Trigger Validity True The parameter True indicates for how long the condition must be fulfilled be fore the gt Trigger command is executed The parameter True is used as a criterion for the truth of signals e g to differ entiate between true signals and spikes If the parameter TRUE is used in combination with the parameter DELAY the command is only executed lt TRUE gt lt DELAY gt seconds after the condition is fulfilled Ej Trigger Delay The parameter Delay refers to the delay in seconds This
17. on this topic Industry Minute An industry minute also referred to as decimal minute has 100 units instead of 60 seconds 2 500 industry minutes therefore means 2 minutes 30 seconds and 2 100 means 2 minutes 6 seconds etc Injection Time The column Inj Time Date shows the day month year hour minute and second when the sample was injected please also see gt Time This column cannot be edited The Dionex Data System enters the injection time of the sample in the corresponding column of the sample list The kind of entry empty or time value indicates whether and when the sample was processed Caution The time difference between successive samples is generally the analysis time plus the time required for injecting the fol lowing sample As the injection time is generally minimal the time value provides a reliable indication as to whether the sample batch was processed without incident A 16 Glossary Isocratic A solvent flow of constant composition is called isocratic This may be either a pure solvent or a solvent mixture of constant composition The DX 80 lon Ana lyzer delivers only pure solvent Jump to Another Help Topic As soon as the mouse cursor is positioned on a link term it changes into a pointing hand All link terms are displayed in green Terms underlined by a dotted line e g gt Dongle are links to additional infor mation in a pop up Terms underlined by a single line are
18. From the Printer Layout The Printer Layout allows you to print the report template at any time e Select Print from the File menu to start printing e Click Preview in the Print dialog box to preview the layout of the printed output The printed output uses the data of the current sample with the layout of the currently opened sheet From the Browser The Browser allows printing single samples and entire sample series e Use the mouse to select the samples to print If you want to print all sam ples of one or several sequences just select the corresponding se quence s e Perform a gt Query to use samples from several Sequences e Select Batch Report from the context menu and select the Report Defi nition to be used for the printed output Also specify the printer In addition to the results it is possible to print sample data the information in the sample list e Open a sample list and choose Print Table from the context menu How to 67 68 How to How to 69 Actions in the Server Configuration Program The following topics contain useful information about the installation of the Dionex Data System software al Changing the Server Configuration Al Selecting the Copy Protection Location and Entering the Key Code al Updating Moduleware Before connecting new chromatography components to the data system read the following topic al Adding Timebases Instruments If a device is connected to th
19. H If the cursor is positioned on a query the Dionex Data System acts as when displaying a sequence If the query is located in the left window section of the gt Browser the sample query is immediately executed The result will be dis played in the right window section If the query is located in the right window 100 How to section of the Browser pressing Return or double clicking the file with the left mouse button will execute the query A previously saved query can be edited via the Properties command on the File menu or via the context menu System Wellness System Wellness provides built in diagnostic and calibration features that help prevent unscheduled system shutdowns and ensure reliable operation of the DX 80 lon Analyzer For an introduction to System Wellness features see al System Wellness Overview System Wellness tasks are performed from the Browser See the following topics for details Al Opening a Wellness Control Panel Al Viewing and Restoring Calibration Data nl Performing Device Calibrations Also see the following topic n Enabling Disabling System Wellness Functions Opening a Wellness Control Panel 1 In the gt Browser under the local gt datasource open the Panels folder and then open the Wellness sub folder 2 Double click DIONEX_DX80_WELLNESS PAN the Wellness control panel for the DX 80 lon Analyzer 3 The control panel opens The Dionex Data System then attempts to connect to th
20. Hardcopy or online batch results are printed see Al Printing In contrast to the workspace a report definition can be used for individual win dows The report definition is required whenever a window is not opened via the workspace The most recent report definition will be opened If no RDF Files have been saved the default report definition supplied with the Dionex Data System DEFAULT RDF is used It is located in the REPORT directory e Select Save Report Definition from the context or View menu to save the current settings e Select Load Report Definition from the context or View menu to open a previously saved report definition Create Open Files Windows and Templates There are several ways to open or create files windows and templates Fre quently other information is required for this For example each time it is opened the control panel searches its gt Time base i e the correct link between the client PC and a certain chromatography system and each method window searches data from a specific gt Datasource B Notes If a problem arises in this context it may be because a cor rectly installed timebase or datasource is later renamed by a user Similar problems may occur if a database is located on a network PC to which no link can be currently established or for which the user has no share authorization In this case refer to How to AJ Actions in the Browser Setting Up a Datasource Co
21. In contrast the server will continue operation even if a user logs off the system Problems occur when the server is to perform operations via the network If the server should process sequences located on a network datasource a special procedure may be necessary under Windows NT The server must be assigned a user account for its network access This is only possible if the user has ad ministrator rights Proceed as follows e Click the Services icon on the Control Panel e Select the Chromeleon Server item from the list and click the Start button e Caution do not modify the Chromeleon Driver entry e Inthe Services dialog box activate This Account Enter a user name and password with access to the corresponding network directory Use the but ton on the right of the edit field to choose a user name from the list B Note The selected user must be a member of the CHROMELEON Installation Software 19 Operators group lf users must change their passwords at regular intervals it is recommended that you establish a separate account for the server this eliminates the need to change the passwords for the Dionex Data System server service at regular intervals After completion and restarting the system the Dionex Data System server is automatically logged on under the new user account Software Protection Each Dionex Data System station regardless of whether it is a server and or a client is protected by a copy p
22. OQ is performed following the in Stallation of a new device or following a repair or system service The Qualification menu of the Dionex Data System Browser includes the In struments OQ and gt OQ Setup commands Select Instruments OQ to per form the operational qualification Select OQ Setup to generate the templates required for performing the operational qualification Normally this is only nec essary after a new installation or when the configuration has been modified The individual report pages of the Instrument OQ correspond to the ser formance Qualification pages for instruments However the limits valid for OQ are stricter than those used for PQ OQ See gt Operational Qualification OQ A 20 Glossary Original Size See Full Size Overlay see Chromatogram Overlay Overview Window When zooming an overview window is displayed in the upper right corner of the signal window The overview window shows the entire chromatogram and indicates the currently enlarged section Panel In online control the control and display elements combined in one window are referred to as a Control Panel The control and display elements allow control ling and or monitoring a gt Timebase The DX 80 lon Analyzer timebase is Dionex_DX80 PAN Also see How to n Actions in the Control Panel Peak Summary A Peak Summary lists peak and sample variables of any required samples in a table Generally it includes sampl
23. Removable Media n Locking Datasources Directories and Sequences n Opening Protocol Data Audit Trails n Creating Backup Files al Restoring Backup Files How to 85 Locking Datasources on Removable Media gt Datasources can be installed on removable media a floppy disk Zip disk CD ROM etc in addition to the hard disk of either the local PC or network PC e Select the Mount Datasource command on the File menu and select a drive or search for the datasource via the Browse command The Dionex Data System will automatically show the Windows drive letters of all currently available removable media e If the Dionex Data System finds an existing datasource on the selected media this datasource is automatically connected and is displayed in the Browser e If the Dionex Data System does not find a datasource on the selected me dia the user can install a new datasource on this drive The datasource will be given the name of the drive D Tip Datasources in the Microsoft Access database format can be copied to the removable medium Simply copy the DATA di rectory of the local datasource of the corresponding subdi rectory of a network datasource to the removable medium A Caution If a connection is established to a datasource on a removable medium via the Mount Datasource command do not re move the medium until the connection is correctly terminated via the Dismount Datasource context command Other wise the ope
24. Right Button eee Keyboard 2 2 2 c0sccccessscnscceaccescesscenes Move Ke Peak Delimiter Sample Oriented 0000000000000 Multi Tasking ccccceeeeeeed Using Shortceuts eee Multi Threading 0000000000000aaaaaeaae Operational Qualification My Computer G01 6 6 0 ic egene Dionex Data System 00008 Eege N Original Size 2 2 222220000000000 Name Overlay WEE Sample Vaiable Chromatogram cccccccseeeeees Trigger oo eeceeeeeeeeeeeee A 37 A 38 Overview vi tee eiedeegeegn eeegteiegteeet Online GIP eeste User Manual Overview Window Page Setup Printing with a Report Template Peak Assignment ooooonon0000e00eaeea Peak Delimiter ccccccceeeeeeees Peak Eien Peak Properties cccccceeeeeeeees Peak Siarls Peak Summarg KEE eege EIERE eege PEO OT AT Eeer Program Start Programming ceeeeeceeeeeeeeeees Protection Power Failure 4 Protocol scce Protocol Data Pump COMUMUG schvevusdescehterdaticerreetcectcas e EE EOLO eege Examples Dialog Rosi Examples Wizard enonnnennnn eer E Saving a Query Fe PVC AL CMs eech Raw EE POC QUIS le EE COMMOTES SION eege Ree ee E EE Storage Power Off Case 47 RDF Err Index Ready Occ eee nn eee eee Reconnect eee ee cee eececeececeececeececess Remote oput sisscccaccasesarcca
25. SPX option under Settings Control Panel Network Configuration Properties NetBIOS If it is still not possible to establish a connection contact Dionex for assistance Problems with Controlling Instruments In addition to the control mode the Dionex Data System offers the Monitor Only mode If a user establishes a connection to a timebase via a network the user receives the monitor only status on this timebase The monitor only status is also assigned when a user attempts to access a lo cal timebase that is currently in use by an external user Deactivate the Monitor Only command on the Control menu to enable complete control of the timebase How to 79 Using Recording Demo Data Simulating Data Acquisition Demo Mode lets you simulate data acquisition by loading a pre recorded demo file and then displaying the data from the demo file in the gt Panel s signal win dow The demo file is read back as though the data were being acquired in real time 1 IS JS To select Demo Mode start the gt Server Configuration program and select the DX 80 lon Analyzer under the timebase Select Properties from the Edit or context menu Activate the Demo Mode for the DX 80 on the General tab page Select the demo file from the list Open the DX 80 control panel DIONEX_DX80 PAN connect to the time base and then turn on data acquisition The Data Acquisition dialog box appears The demo file begins running and will run
26. Select Native SQL to enter the SQL statement directly in the SQL syntax of the corresponding ODBC driver contains translated Dionex Data system statements e Select SQL to define the search statement in an entry dialog box e Select Result Restrictions to limit the resulting sample list via further re sult queries 90 How to For details refer to the following topics al Entering the Sample Query Using a Wizard al Selecting Search Criteria H Examples Wizard n Specifying the Sample Query Using the Dialog Box A Editing SQL Statements n Examples Dialog Box n Saving a Query Entering the Sample Query Using the Wizard On the first Wizard page specify whether the gt Query will be performed in the currently open Data Source Selected Datasource or in a different one Click the corresponding option to open the combo box and select the desired data source from the list In addition you can define the criteria types for the query e Sequences e Samples e Results variables from the different report categories On the following Wizard pages use Data Field to choose the variable whose property you want to find Enter an operator and define the value also see il Selecting Search Criteria The combo box to the right of the respective line allows you to enter a logic connective with another search property Only after you enter AND or OR can you enter a second search property Click Finish to complete the Wi
27. The Dionex Data System features the typical Windows properties and capabili ties of a two button mouse Use the left mouse button to point and select menus menu items and icon buttons to operate display and control elements and to modify windows in crease or reduce the size of a window zoom modify display and control ele ments etc Double click the left hand mouse button to execute the selected function Press the left mouse button and the SHIFT key to simultaneously select sev eral cells in a table Select the first cell with the left mouse button press the SHIFT key and select a new cell All cells between the two will be selected Use the left mouse button and the CTRL key to select cells in a table that are not adjacent Select the first cell with the left mouse button press the CTRL key and select a new cell Keep the CTRL key depressed and repeat until all required cells are selected Click an object with the left mouse button and drag it to the desired position Drag and Drop Thus you can move selected text items or entire samples Perform this operation while pressing the CTRL key to copy the corresponding object The right mouse button is reserved for opening context sensitive menus These menus contain functions that are required or allowed in the current situation For further information please refer to your Windows manual Undo Redo Commands The Dionex Data System remembers the user s last modifica
28. Trails cccceeeeeeeeeeeeeeeeeeees DD E Creating Backup tes SL E Restoring Backup Files cccccccceccceceeeeceeseeeceeeeeeeeeeseeeeseaees Contents Vv Performing a QUCTY ccccccceeeceeeeeeeeeeeeeeeeeeeeeeaeeeeaseeesaneesaneeseeneesoaeees 89 E Entering the Sample Query Using the Wand H Selecting Search Criteria cc cecccccceseceeceeeceeeseeeeesseeeeesaees H Examples Wizard EE 94 E Specifying the Sample Query Using the Dialog Box H E Editing SQL Statement cc ccccccceececeeceeeceeeeeeeeeseeeeeeeeaees 96 Examples Dialog Box H Saving ir Ref E System WellnessS eessen E Opening a Wellness Control Panel E Viewing and Restoring Calibration Data E Performing Device Calibrations cccccccccseeceeeeeeeeeeeeeeeeens 101 E Calibrating the Pressure Transducer Offset 102 E Calibrating the Conductivity Cell ee ceeccceceeeeeeeeeeeeees Actions in the Window Section Chromatogram ccesesee Manual Re Integration ccccccssseeseseeneeeneeeeseeeneeeeneeeneeeeneeseneess 104 E Moving Peak Delimiters ccccccccecseseceeeeeeeeseeeeeeseeeeeeeas 105 E Modifying the Baseline ccc ccccecccccseeceeeeeeeeeeseeeeeeesaeeeeeeas 105 Inserting E Manual Peak ASSIQNMeN1 cccsceceeeeeeeeeeeeeeeeeeeeeeeeenessanessenenes Performing a Chromatogram Comparison ERKENNEN Actions in the Window Section Rep
29. and they have no file history Use the gt Restore command see How to Al Actions in the Browser Restoring Backup Files to restore backup files that were created via the Backup function Baseline The line drawn from peak start to peak end for calculating the peak area is called the Baseline D Tip In colloquial language the chromatogram section between two peaks is also referred to as the baseline Glossary A 7 Batch Processing Repeating a processing step several times is referred to as batch processing also called batch or online batch In chromatography this means automati cally processing or analyzing several samples in a defined order For details see Al Samples and Sequences Online Batch Browser The Dionex Data System Browser enables you to set up delete and move gt Datasources subordinate directories and files The Browser displays samples methods and programs as well as additional details about a gt Sequence The functions and the structure are similar to the Windows Explorer Open the individual files by double clicking Caution The Browser functions and structure are similar to those of the Windows Explorer However do not confuse the Browser and Windows Explorer Do not use the Windows Explorer for operations within Dionex Data System datasources For further details refer to H Browser Chromatogram Overlay select Chromatogram Overlay to display an earlier chromat
30. can also be integrated as a window section in a method window Window sections with a graphical representation show the coordinates of the mouse cursor in the status bar Table Editor The table editor is used for representation and entry of various parameters and variables This includes creating sequences in the sequence table and the rep resentation of all result variables e Appearance and use of all tables correspond to the Windows conventions Editing is by cell column or line e Selected columns can be moved hidden or made visible at the current cur sor position The column width is adjusted by moving the left or right delim iter or by applying the function Optimum column width e Unknown samples only Adding lines to the table e Unknown samples only Removing selected lines from the table e The described functions can be accessed via the right mouse button Basic Operation 32 e The font size can be changed For example to display more information on the screen select a smaller font e Press the F1 key to view online Help about a specific column e Press the F8 key to open an Edit dialog box for a specific field This pre vents the input of incorrect or invalid values and names e Press the F9 key to fill an entire column with the value of the current field Logical rows of numbers can also be extended with this method If the input is 2 4 and 6 in the first three fields the row is continued with 8 10 12
31. command from the Server menu to start the check The Dionex Data System shows the result in a separate dialog box If no errors or conflicts occur the current server configuration can be saved If the system issues one or several warnings the user must decide whether to change the current configuration If the system issues an error message the cause must be found and remedied before optimal operation be ensured Installation Configuration Report The configuration report contains information about the server itself eg name key code copy protection as well as the name of the connected time base s In addition the report lists the settings of the instruments assigned to the timebase e Select the Create Report command from the Server menu to generate a report e Click the Save button to store the report in the CMINST LOG file The old file is then renamed CMINST OLD Caution The report is always saved to the BIN directory of the server e Click the Print button to copy the report to Windows Notepad and to print it from there After creating the report it is also on the Windows Clipboard and can be inserted in other applications How to 73 Selecting the Copy Protection Location and Entering the Key Code You may enter the software protection location and the gt Key Code for either a server PC or a client PC In the case of the client PC select Preferences on the File menu of the Dionex Data System client The L
32. continuously until data acquisition is turned off Recording Demo Data 1 To create a demo file for the DX 80 open the Properties dialog box in the Server Configuration program and select Write under Demo Mode on the General tab page Enter a name for the demo file Select the Device ID under Communica tion To write the demo file open the DX 80 control panel DIONEX_DX80 PAN and turn on data acquisition The data acquired from the DX 80 is recorded in the demo file To stop recording data turn off data acquisition The demo file is then com plete 80 How to Actions in the Browser Use the SI Browser to manage copy move and delete chromatographic data and files Ay Warning The Browser functions are similar to those of the Windows Ex plorer However do not confuse the Browser and Windows Explorer Renaming or moving files in the Windows Explorer makes them invisible to the Dionex Data System Thus the files could be unintentionally deleted In addition the Browser allows creating a sample list setting up datasources searching and selecting data in a database For further details see the topics below H Creating a Sample List Sequence Table al Setting Up a Datasource n Handling Files Datasources and the Network nl Performing a Query nl System Wellness Creating a Sample List Sequence Table There are two ways to create a al Sample List manually or automatically Manually e Select a s
33. correction factor for the amount calculation formulas As the injection volume it can be used in multiple point calibrations to define the dilution of subsequent calibration samples For integration samples it serves to account for any dilution that was made before injection The calculated result is corrected by the appropriate dilution factor for all peaks of a sample Caution The effect is reciprocal to the injection volume i e if the di lution factor of a sample is larger than that of the previous sample the sample contains less of the component than the previous one Glossary A 13 Disconnect Select the Disconnect command to separate a user PC client from a time base The specified instrument is no longer monitored by the data system nor can it be operated via the data system D Tip Select the Connect command to undo the operation Dongle A dongle is an adapter that is placed on the parallel PC interface The free end of the adapter is designed as a parallel interface connector and thus provides the parallel interface functions The dongle stores the serial number of a Dionex Data System station Each station has its own serial number The Dionex Data System be operated correctly only if the serial number of the dongle and the gt Key Code stored in the Dionex Data System match If there is no dongle or license server or if the key code is incorrect the Dionex Data System can be operated only in demo mod
34. links to a new Help topic Key Code The range of functions available for an individual Dionex Data System station is determined during installation by a key code Each Dionex Data System station is fitted with a gt Dongle the station can be operated only if the serial number coded in the dongle matches the 12 digit key code Each user who expands the Dionex Data System scope of functions by ac quiring the corresponding licenses receives a new key code The extended scope of functions becomes available as soon as the new code is entered Enter the key code via the About box on the Help menu or via the gt Server Configuration program Valid characters are numbers and letters The input is not case sensitive For further details refer to the sections Installation Al Software Protection and How to Actions in the Server Configuration Program Selecting the Copy Protection Location and Entering the Key Code License Server If a site includes a large number of computers the Dionex Data System li censes can be managed by the optional Dionex Data System License Server With this type of installation it would not make sense to equip all client PCs with gt Dongles and to enter the corresponding gt Key Codes If no dongle or License Server is specified the Dionex Data System can be op erated in the demo mode only Glossary A 17 For further information refer to the following Installation 4 Software Protection and
35. oc curred during sample processing For more information see the following topic in the chapter Data Al Protocol Data Audit Trail For documentation purposes single events can be included in a report For details see How to Actions in the Window Section Report Al Audit Trail Browser The Browser displays the directories in which chromatographic data are lo cated The Browser allows opening moving and deleting chromatographic data as well as searching for specific data in various databases When a file is selected it can be opened and its contents can be viewed in a separate win dow Caution The Browser functions and structure are similar to those of the Windows Explorer However do not confuse the Browser and Windows Explorer Do not use the Windows Explorer for opera tions within Dionex Data System datasources For more details see the topics below X Common Features with the Windows Explorer Al Differences from the Windows Explorer Al Function Common Features with the Windows Explorer The Dionex Data System Browser window has two separate sections As in the Windows Explorer the directory structure of all selectable directories is dis played on the left Detailed information on files and gt Sequences is shown on the right Operation is also identical to the Explorer e Click the and symbols next to each directory name to expand or collapse the subdirectory structure e Selec
36. the following Samples smp_inject_time gt CURRENT_INTERVAL 0 DAY To restrict the search to the current day s standard samples instead of all sam ple types the SQL query must also include the following input Edit Condition 98 How to The SQL sheet now shows the following Samples smp_type S AND Samples smp_inject_time gt CURRENT_INTERVAL 0 DAY To search for all samples in a sequence named 7709 in which the peak Ben zol benzene or a peak of the PAK PAH peak group was identified first enter the following Edit Condition A SW Sequence Name Z pekne a 2 Deel How to 99 Finally enter the condition or PAK PAH in the Result Restrictions box Edit Condition Change Condition C Restrict Condition AND if Sei D Ge Expand Condition OF Cancel Formula peak group Operator Value PAK The SQL sheet will now show Sequences seq_name S7709 and the Result Restrictions sheet will indicate peak name Benzol OR peak group PAK For examples on entering the query using the Wizard see Al Examples Wiz ard Saving a Query Queries can be saved as a file in the datasource For this purpose open the gt Query Wizard via the File menu or via the right mouse button After entering the conditions click the OK button to save these conditions as a query object under any file name In the Browser the gt Query is indicated by the following symbol
37. the network A client a server and a local datasource are set up on each PC during the Dionex Data System installation Independently of this each PC can act as client only or server only Theoretically each client can access each data source and each server However in practice this may not be desirable for safety reasons Therefore there are various ways in which to restrict user ac cess e The gt Server Configuration program allows you to define the extent of net work operation for each gt Server and for each timebase A server can be made available for the entire network or the server can be partly or com pletely locked If the server is partly locked monitoring the server is possi ble but control of the connected instruments is disabled In this case con trol is reserved for the local client This distinction also applies to the time bases If three timebases TIME1 2 3 are installed on a server TIME1 could be completely shared TIME2 could be excluded from network opera tion and TIME3 could be shared for monitoring only Hardware and Software Chromatography Components 11 e Datasources and or their subdirectories can also be protected from unde sired access Depending on the location of the corresponding database on the local hard disk or a network PC the respective user or the network ad ministrator decides by sharing a directory which data can be accessed and by whom In addition datasources shared
38. the sample type Matrix Blank and e whose comment starts with Charge 123456 or e aninjection date between 2 2 2000 and 3 2 2000 WW Query Wizard Samples Data Field Operator Value Sample Type sl Matis Blank ano sl Data Field Operator Value Sample Comment sl starts with Charge 123456 sl OR Data Field Operator Value Sample Inject Time sl is between 02 02 2000 00 00 00 D 07 03 2000 23 59 59 e sl Data Field Operator VWalue March 2000 sl Sun Mon Tue Wed Thu Fri 25 29 1 ep 3 boas 9 13 14 15 16 lt Back 20 21 22 23 27 20 29 30 3 4 5 P 3 Today 3 7 00 Use the following settings to restrict the query to samples e containing an Anthracen peak or e containing more than 10 calibration points How to 95 Results eee Query Wizard Result Restrictions Formula Operator Value Iech name fF Anthracene AND Formula Operator Value peak nCalpoints Fi l d Ir U Ge e Formula Latimer E Categones Variables Formula Calibration Mode Auto Recalibrate Reference Inject Volume Calibration Type Wi enghts Offset CO 8 Slope C7 JCupeelt A Cancel Lcpstomsge RF Yalue Anmount4rea Number of Calibr Points Number of disabled Calibr Points Explain Variable Vitara Forrnula peak nCalpaints Faremeten For further examples on restricting the query see al Examples Dialog Box El Specifying the Sample Query Using
39. time delay and it is not possible to determine whether the process is performed on the local or on a remote PC RPC requires a functioning network connection between the involved comput ers The computers may be part of the same network or may be linked via a modem connection Communication also functions between different networks such as Novell or Windows provided the networks communicate via a com mon protocol A 28 Glossary The Dionex Data System generally communicates via the network protocols IPX SPX TCP IP or NetBEUI Under Windows NT 2000 the protocol Named Pipes is also valid Sample Data Data that contribute to characterize a single sample are stored in a database by the Dionex Data System These data include not only the entered data name etc but all results calculated from the gt Raw Data and the chroma tographic conditions under which the sample was analyzed column solvent etc Sample Name The gt Sample Variable Sample identifies a sample and is used to label graph ics and result reports When creating a sample list with the al Sequence Wizard automatic sample name generation is possible Sample Number Nr The gt Sample Variable Nr indicates the sample number for a particular sample row and is used to label graphics and result reports Sample Oriented Operation Due to the object oriented concept of the Dionex Data System emphasis is on samples and gt Sequences not on methods or a
40. to AJ Actions in the Server Configuration Program Changing the Server Configuration A 32 Glossary Server Monitor Program The program required for starting and monitoring the chromatography server is called the Server Monitor During installation it can be included in the Autostart group of the operating system When starting the Server Monitor an additional icon appears on the Windows status bar indicating the current status of the server e Move the mouse cursor over the icon to view the server status via a quick info field Open the corresponding context menu via the right mouse button to start or to stop the server Start Stop Server or to exit the monitor program Quit Monitor Double click the icon for further options e g to automatically start the server when booting the computer The icon s color also indicates the server status Icon Color Message Crossed PeakNet Server is not running out red Yellow PeakNet Server is starting is closing Green PeakNet Server is active Tur PeakNet Server is reconfiguring quoise Gray PeakNet Server is running idle sa Yellow PeakNet Server needs attention E The server waits for the response to a message For example after a power failure the user must decide how the program should continue the interrupted sam ple processing Glossary A 33 Signal The data either analog or digital transferred directly from an instrument to th
41. to the timebase s installed on the server To manage database access the Dionex Data System uses the capability of modern Sil Operating Systems to share resources Thus a client can access all files or databases for which sharing was issued on a different workstation It is only possible to monitor and control a system via the client To do this the client must establish a connection to a chromatography gt Server In combination with a I Network this allows operation of a system as well as online representation of the system status and the current chromatogram course from any client Synchronous control of a system via several clients is not possible Glossary A 9 Comment In addition to the sample name further comments can be entered here Press the F8 key or double click to access the enlarged edit field Connect Execute the Connect to Timebase command to connect a user PC gt Client with a timebase gt Chromatography Server In a network installation a con nection can thus be established between the current client and any server within the network Select the Connect Device command to connect a device with the server to enable remote control The command verifies that the specified device is actu ally connected and then activates the instrument For all installed instruments the Connect command is automatically executed when the program is started Contents Button The Contents tab dialog box in the onli
42. topic under How to Actions in the Window Section Report Manual Re Integration Manual Re Integration includes all actions performed directly in the chroma togram via mouse click by means of the Automatic Tool on the Integration gt Toolbar This includes the actions described in the topics below al Moving Peak Delimiters n Modifying the Baseline n Inserting a Peak Prerequisite An integrated chromatogram can be recognized by the red baseline below the peaks and by the peak delimiters on the left and right of the baseline integration limit Caution These elements must be displayed in the chromatogram see Context menu right mouse button Decoration Peak Decoration Before performing any manual re integration it is helpful to zoom the area in which you want to perform a modification Select the Automatic Tool on the How to 105 Integration toolbar to mark each place in the chromatogram that you plan to modify manually The shape of the mouse cursor indicates which action is cur rently possible All modifications to the chromatogram can be undone provided that they have not been stored On the Edit menu select the Delete Manipulations com mand Moving Peak Delimiters When the Automatic Tool is selected the mouse cursor changes its appear ance near the blue peak delimiters The cursor shape becomes peak start L or peak end TL Press the left mouse button and move the mouse cursor
43. CNAME_1 that serve to save default templates A default gt Datasource is also created The underlying database is located directly in the DATA directory under the name CM _LOCAL mdb Datasources are visible only in the Dionex Data System gt Browser see the Data section The environment created by the Setup program has the follow ing structure The local datasource has the name lt PCNAME local gt It contains the PCNAME _1 subdirectory in which the gt Sequence manual is located The sequence which consists of a single default sample enables manual data ac quisition To access default templates directly in the Dionex Data System sub directories of the DATA directory are mapped PANELS REPORT Modifications to the described directory structure are realized by creating new e Datasources and sequences in the Browser e Timebases in the Al Server Configuration Program Caution If the Dionex Data System is not started correctly there may be a version conflict between the system files in the Windows system directory In this case install MDAC_TYP EXE once again The AJ Installation Qualification Manager provides an overview on the version numbers used Installation Software 17 Dionex Data System Update Updating the Dionex Data System is started by the SETUP EXE file Ke Note When updating the Dionex Data System version uninstalling a previous Dionex Data System version is not required and not recom
44. Dimethyl Fluoranthene 1 796 min Retention rman ae if Phenanthrene d r Tetracene H Anthracene Peak Type y Fluoranthene Height 7 Pyrene Area Amount 2 317 min e tis also possible to rename an identified peak by manually assigning it a different name Enter the new name or select one of the names listed in the combo box and then confirm your entry by pressing the first symbol button Via the additional symbol buttons you can cancel this action or delete all manual peak assignments see quick info for the buttons Man ual peak assignments of identified peaks are not transferred to the QNT method they apply only to the currently open chromatogram Properties of Peak No 5 Component Phenol M EI Retention SEE Width U 24 Peak Type BMB Ia 1715 62 oe 282 37 mount 49 268 min mir S RK ls You do not have to close the dialog box to continue working in the chroma togram The dialog box remains open until it is closed or until the chroma togram is closed 108 How to Performing a Chromatogram Comparison Comparison is always between one or several channels of one or several sam ples The channels must be selected and placed in relation to each other i e they must be overlaid Selecting channels Or Or Or Or Display the chromatogram of a sample in an integration window by opening the sample e g double click its name in the gt Browser Select one o
45. EE eege 19 17 Keyboard o cccccccccescsecseeseseeseseees 115 Operation EE ee Updating Moduleware nnnnnn Layou erleedegen License Server Eeer License Type Sorucu ene e MAD i seas elses AT geist A Named PIPES acercetenidcoeeteoece rice tttatesceces Pressure NETBEU ebe eege Tripper siccoaccasaiuncensiavnceninensounsecrse Netware Trigger Commands aa000000 Load e e E Autosampler E ee Eege SE ND asdisran ee Installation n n Log Commande Local Client Server Installation Log File CMSETUP LOG VEVI CW sco retcecteee tr eceertoercastetideceeaee LOST orie Eegen Lower Limit Pressure Server Configuration Network Neighborhood te EE M Signal to Noise Ratio Manual User Manual Se a ee IN GV HE Manual Peak Assignment Manual Re Integration 006 O eegen Method Wimdow 31 ODBC Interface wo cececeeeeeeeeeee Microsoft Network ODBC Manager Millennium Compliance Online Batch ceeeeeeeeeeeeeeeees Mode Print oo e ce cecccecescesceseesessesseseeseesees Monitor Only Online Help A 9 A 14 A 15 Moduleware sisiseeieieieeeeeeee A 16l A 19 Monitor Only Online Wmdow Monitor Program EE Mount Datasource Operating Swvstem IV GUIS 2 ac ceneodeutacesceoataedeusacuedeneataesaceanze EIERE os encsceesainadarsaeusctesacueseeaest Retter geesde Seege Data System cece
46. Goftware kk N NENNEN NEEN E RANNER NEEN NNN Tips for Software Installation 0 cccececeeceee esse KEREN KEREN REENEN Initial Installation and Update eee eeec eee ceeeeeeeeeeeeseeeeeeeeees The Dionex Data System Under Windows 98 NT 2000 Software Protection eege egene gege EES Server Configuration Hrogram ERKENNEN ENER ENNEN KEREN KENE Contents BASIC OPO ge VE Operation via the Keyboard ccsceceseeeeeneeeeeeeeeeeeeeeseneeeeneseenenes Operation via the MOUSE cccccescceeeeeeeeeeeeeeeeeeseceneeeeeceeeeeneseeeens Undo Redo Commands cccccceecceseeeeeeeeeeeneeeneeaneeenesenesaneceesseeees teen E The Client User Interface cccsccceeeeeeeeeneeeeeseneeenesenesenesenesenenens Nudelen GCGo ontrol IT ME 26 H Function ccccccccccccececececcececsceccecacscaccucececscaucucacseacusecseaeass 26 BB Audit Tra OWS eege E Common Features with the Windows Explorer 239 E Differences from the Windows Explorer 29 Tafele Method Klee Te EE TADO D de EE User Profiles Workspaces cccceseeeeseeeeeeeeeeeeeeeseeneeseeneeseneeseneees Report Definition EE Create Open Files Windows and Templates s ccseseeeeeeees e dl de BE B ef o EE Contool CONCEDII EE ei ge GC CCU S E E A EN E E E 36 H System Commande uk Ki E DX 80 lon Analyzer Commandes BW General Commande 3 E PGMM File oo cssecccscccsseesssecssecsssecssecssseesse
47. Standard sample s Determine Calibr curve known amount peak area calibr coeff ll a Analysis sample s Determine Amount A peak area Amount substance A The number of peaks that are detected and whether any of the peaks can be identified depends on whether an anion or cation analysis is running The results of the two processes area calculation and peak identification are represented graphically chromatogram and as a table Report in the window of the partial method Integration Integration 61 For more information see the topics below nl Operation Al Integration Report 5 Peak Summary Report Al Chromatogram Comparison E Operation Opening Opening a sample automatically opens the integration method window and dis plays the sample chromatogram There are three options e Double click a sample of a specific sequence in the Browser or e Select a sample in the Browser and double click or e Select File gt Open and then select Sample in the Object of type list box Modifying the Appearance Each displayed chromatogram can be changed by enabling and or disabling the display of various elements and by modifying them e Double click a peak to see the Peak Properties e Draw a frame around the section of the chromatogram that you want to en large e Select the Decoration command on the context menu right mouse but ton to modify the window background the different axes th
48. System will result in a loss of data Therefore we recommend that you protect your datasources to prevent them from being accessed via the Windows Explorer In the file or context menus of the Browser activate the Protect Data Set Directory option on the Properties tab of the cor responding datasource Raw Data Compression Storing raw data automatically compresses the data For the signal value this is achieved by storing the difference from the next data point instead of storing each data point The actual value is stored only periodically This increases the compression by 50 D Tip The compression procedure is not destructive complete data are stored and can be restored at any time Restoring a Chromatogram from Raw Data When restoring a chromatogram from the raw data equidistant data points are joined with straight lines Thus a diagram resembling the recorded analog signal is created Data 46 Raw Data Export The Dionex Data System supports the export of raw data by conversion into the gt AI A and ASCII formats In addition raw data can be exported as a Dionex Data System backup file cmb Use the Export Backup command on the File menu to export as an AIA or backup file Format Description AlA Cat 2 Samples and peak variables are stored in the AIA format AIA Cat 1 2 In addition to samples and peak variables the raw data of a chroma togram all stored data points are stored in the AIA format Dionex
49. ace Flow License Server un Continue e ec eeeeeeeeeeeeseeeseeeeeee Program ant EE Ee EE Server Monitor Program EEN DOMES eeh be ee E E A Direct Centre Gegptgeestsshaesgbegeesg System Commande Eege G Bec eens eee nen nee he A Conceal a ERE E DISCONMECE cc2csecercncectwnscssaereieceeieussd Generating a Reen 110 IV Hysteresis ccnneocsssiupaonnseubacdsnenendatdead TOT EE Trigger Commande Inj WA Cat soraia Inj Prec and Ret Repro Inject Autosampler Injection NK te Insert Peak Tool Installation Actions in the Control Panel Actions in the Server Configuration Program 69 Adding Instruments Adding Timebases c00ccccce0e Changing the Server Configuration cccccceeeeeeeee Client Server Installation Selecting the Copy Protection TO CACO eccere Index Server Configuration Program 20 Software Protection ccceeeee Time ER Tips for Software Installation Installation Qualification Manager Installation WE EE eegene Actions in the Window Section Chromatogram ccee 104 Actions in the Window Section EE eege Chromatogram Comparison Integration Report Manual Re Integration OPETAUON iesen OS Tlie Ate Peak Summary Report nnnnnnnn Performing a Chromatogram Compartson 108 Integration Report 110 Ee gaan eee ne ae 110 Interface Server Configuration EE eeben EN
50. al separation procedures liquid liquid extraction or fixed phase extraction As a general rule careful sample preparation contributes substantially to the quality and reproducibility of a chromatographic separation Furthermore the gt Dilution Factor allows the user to define dilution steps and can thus be used to consider the sample preparation when evaluating the data Sample Processing Sample processing includes three major steps e Sample definition single samples and sample series e Analytical procedure manual or automatic control e Evaluation methods and reports The performance of each step depends primarily on the methods used and the instruments available The working environment could range from a fully auto matic sample laboratory with large quantities of samples to single user applica tions in a research lab Easy and quick analysis procedures may be the focus in the first case while special methods and parameters for peak recognition may have priority in the latter case Thus functions crucial to one group of us ers may be irrelevant to others Please take these facts into account when information is presented from differ ent viewpoints in Help topics Sample Registration In the Dionex Data System the term sample has a more specific meaning than in colloquial use Samples and Sequences 50 Each injection is defined as an individual sample Multiple injections from the same sample vial under sim
51. alibration Wizard automatically appears when PeakNet IA is loaded for the first time You must calibrate the DX 80 before beginning operation The Calibration Wizard guides you through the following steps Selecting the ion mode the number of calibration levels and the injection method gt Step 7 Creating a new calibration sequence or copying an existing calibration se quence gt Step 2 Selecting a timebase for the new calibration sequence gt Step 3 Saving the new calibration sequence gt Step 4 Each step is performed on a separate page of the Calibration Wizard Clicking Next on any page takes you to the following page Click Finish on the final step to save the sequence and close the wizard Automatic Batch Operation Online Batch Independent processing of one or several sequences is known as gt Online Batch When the actual sample preparation is completed the chromatographic condi tions of processing the samples to be processed and the order of processing must be communicated to the data system This is performed in the sequence table The result is stored as a gt Sequence To start online batch processing the following steps are required Starting the Automatic Batch After data input is completed the analytical process can be started in an online batch Open a control panel and choose the Edit command from the Batch menu Enter the names of the sequences containing the samples to be analyzed
52. alibration list for the calibration mode fixed Query Preferred RDF file samples QNT File PGM File Select Backup to open the Backup dialog box For a description of the individ ual fields see gt Backup Before starting the backup the required storage space is determined In the case of hard disks and network drives the Dionex Data System checks whether there is sufficient storage capacity If there is not enough space an er ror message appears to allow the user to cancel the backup procedure 88 How to If using removable media the expected number of media is displayed The user is prompted to keep the media ready If using data compression the num ber of actually required media may differ from the displayed number After that the selected objects are stored A dialog box appears to allow the user to monitor the procedure A progress bar indicates the current status The bar corresponds to the percentage of stored data already copied to the me dium If the destination is a removable medium the user is prompted to change the medium as necessary The backup is indicated in the gt File History The backup can be interrupted at any time by clicking the Cancel button In this case the backup contains all objects stored up to this point If the original objects should be deleted after the backup a dialog box appears asking the user to confirm the action If the history mode is active a dialog box for entering a history co
53. alysis Raw Data Storage Raw data storage refers to saving the signals received from a detector in digital form Other important data are also stored analysis time signal unit number of data points etc Storage Procedure With conventional data systems an analog value is digitized at a fixed time in terval e g a digital value of defined accuracy is stored every second The number of stored values per second is normally referred to as the gt Sampling Rate The DX 80 lon Analyzer sampling rate is 10 Hz The higher the sampling rate the more data points are stored and the more exactly the original signal can be restored from the stored data However a higher sampling rate requires more memory Data 45 Storage Location Raw data are stored in the directory of the current sequence In addition a protocol data directory is created The directories are not visible in the Browser because all raw data are managed automatically by the Dionex Data System At no time can the user directly access the raw data The only way to view raw data is via the Windows Explorer provided that the datasources are not locked However as the datasource names are not dis played in the Browser you must follow the path to the corresponding sequence directory A separate raw data file is created for each sample in a sequence for which raw data were recorded Caution Do not modify these directories Operations outside of the Dionex Data
54. analysis as well as for documentation and archiving purposes The user must determine or enter the data before starting the analysis These include the chromatographic conditions connected devices etc as well as the run time A distinction is made among the following types of data e Data that describes a sample gt Sample Data e Data that describes a sequence gt Sequence Data usually entered auto matically e Data that describes the chromatographic treatment of a sample gt Chro matographic Methods The user input data serves as the basis for the analytical process Output Data The data recorded during analysis are referred to as output data Output data includes the following e Data provided by the analysis process itself analysis and gt Raw Data e Protocol data on the analysis gt Audit Trail Because of the variety of data types systematic data organization and Al Data Storage are very important Data 40 Datasource The term gt Datasource is used for the top level of the directory structure dis played in the gt Browser The Browser is also the tool for handling datasources Each datasource is based on a separate database When setting up a data source the user must enter a path to an existing database or create a new database e Select the Datasources command from the File menu to set up a data source For detailed information on the required steps see How to Actions i
55. and But ton Edit Field Action Esc or right mouse but ton F 1 F2 Alt Enter Ctrl Tab Ctrl Ctrl X Ctrl C Ctrl V F9 Ctrl F Ctrl Ctrl Break Pause Ctrl A Ctrl B Double click Shift Click Tab Enter Esc Where gt Browser gt Panels signal window in the gt Report Overview window Time axis Signal axis Plot range otherwise when Zooming pressed button Reaction aborts Drag Move opens the context sensitive Help activates the Edit mode opens the respective Properties toggle between open windows for zooming changes the cursors to the zoom cursor Cut Copy Paste Fills the column selected cell s with the first value of the selection Flow Command Inject Pump Stop Hold Mode Data Acquisition On Edit Batch Unzoom Auto Plot Speed Autoscale Signals Axis Decoration Retains scale ratio between sig nal and value axis i e the shape of chromatograms is maintained Indicates running program Stops program upon confirmation Sends the new value Aborts entry 116 How to Glossary A 1 Glossary Glossary A 3 Acquisition On Off The Acquisition on command activates data recording gt Raw Data from all selected gt Signals of a timebase Each signal is stored in a separate file The type of data from each signal is determined via signal parameters Raw data acquisition may be interrupte
56. and then click Start The icon will show various color steps according to its status The gray coloring indicates that the server is running and the following message is displayed PeakNet Server is run Basic Operation 25 ning idle From this point you will have direct access via control panels to the devices installed below a timebase Provided the instruments are cor rectly installed they can be operated online B Note Any problems that occur when controlling single instruments may be caused by an incorrect server configuration Start the Al Server Configuration Program to check the configura tion The Client User Interface The Dionex Data System user interface employs standard Windows elements including the menu bar gt Toolbar and gt Status Bar The toolbar display see below is enabled and disabled via the View menu Standard Tool hee t d Ka os Ki bar for various standard func tions Online Tools for b 0 ee using the control panel Method Tools for i selecting a m S E P method window Integration Tools hk D BEZA Gi for the most im portant operations in the Chroma togram window section Move the mouse cursor over a button to view a description of the button The space between the taskbar and the status bar is called the workspace The workspace displays four different Al Window Types Basic Operation 26 Window Types In the Dionex Data System there
57. application etc The client merely receives the search results or the current status of the application Basically this means that the client provides the user interface while the actual operation is performed on the server via remote control The Dionex Data System also uses this division of labor The user starts the Dionex Data System on a local PC the gt Client The user can then perform all server independent tasks including reprocessing of raw data and creating sequences lf a Chromatography gt Server is activated via the gt Server Monitor Program it is possible to control and monitor the chromatography instruments connected to the server Theoretically each client connected with a running server via a gt Control Panel can perform this control In practice the first client using this possibility also has control rights From this point all other Dionex Data System users can only monitor the system status Via device drivers the server converts the control commands entered on the first client PC for the analytical instruments Inversely the server receives in formation from the system and forwards it to the appropriate locations Thus status information appears on all client PCs connected with this chromatogra phy system Raw data is automatically stored in the directory of a gt Datasource and the underlying database Depending on whether the client and the server are located on the same PC or on different PCs on a
58. are displayed the presentation becomes confusing Therefore the Dionex Data System prompts the user to confirm whether to display more than 20 chromatograms This prevents the display of chromatograms due to an operator error and a resulting decrease in system performance How are chromatograms compared and displayed Chromatograms can be displayed in the integration window and compared One chromatogram represents the currently active chromatogram It is selected with the mouse It can be recognized by the additional information displayed decoration etc The names of all represented chromatograms are listed above the window The currently active chromatogram is marked by a different color Clicking the name of any chromatogram selects it as the active chroma togram The presentation of the window and the active chromatogram is controlled via the Decoration command on the context menu How do I select the chromatograms to be compared There are various ways to select chromatograms e Select one or several samples in the Browser and drag them into an open integration window e Select the Add Overlay command from the File menu to display a spe cific channel of any sample in an opened integration window e Select the Compare command to compare a specific channel in several selected samples e Perform a query to specifically compare samples with certain properties e Click the Next Chromatogram icon BI while holding down t
59. are four different window types with different appearances and functions For descriptions see the topics below Control Panel Browser Method Window X Table Editor Control Panel A control panel also referred to as an online window controls and monitors the chromatography instruments of a gt Timebase For details see the following topics al Function Al Audit Trail Function Control panels are used to control and monitor individual gt Timebases The timebase to be controlled is specified when creating the control panel As soon as the control panel is opened the software automatically attempts to establish a connection with the specified timebase If this is not possible e g because the corresponding timebase was renamed or because the corresponding Chromatography gt Server is not in operation a message appears on screen In this case the assignment must be changed manually Proceed as follows e Open the preferred control panel via File Open e Select Connect to Timebase from the Control menu and specify the name of the timebase to be connected with the control panel or select the name from the available list The new assignment is valid until the window is closed Store the window if you want to have the current assignment available next time you open the window The currently selected timebase is displayed in the status bar Basic Operation 27 Audit Trail The Audit Trail in a Cont
60. asource lt PCNAME_LOCAL gt Data 41 Directory Structure of a Datasource Each datasource can be equipped with any number of hierarchically organized subdirectories via the command File New Directory Caution Do not use special characters e g the umlaut for new di rectory names or sequences as this may cause problems on Novell networks Caution Actions performed in the Browser such as creating data sources or directories require complex operations below the user interface and cannot be compared to or performed by the Windows Explorer The representation of directories and data also differs considerably from the Explorer Subdirectories sequences and chromatographic methods can be moved via Drag amp Drop within a datasource but also between datasources of different types This is a copy process that can also change the underlying database If you want to actually move a sample delete the original sample in the Browser after the copy process Caution Drag amp Drop operations can be executed exclusively via the gt Browser Actions outside of the Dionex Data System eg in the Windows Explorer will result in loss of data Locking Datasources Directories and Sequences To protect data and results the Dionex Data System allows the user to lock datasources directories or sequences If the Locked status is enabled it is not possible to modify the corresponding object This also applies to all objects
61. below the locked object If for example a datasource is locked all directories and sequences in the datasource are also locked Locked items can be recog nized in the Browser by the red lock on the icon 8 82 To lock an object e Select the object in the Browser e Select the Properties command via the context menu and activate the Locked check box in the edit box Repeat these steps to undo the operation Data 42 Data Storage In order to ensure fast and efficient access to specific data intelligent storage and organization of the complete data are very important The Dionex Data System solves this problem by storing data and files in different locations Da tabases and sequence directories are available for this purpose Both are part of the i Datasource Storage in a Database Data that can be compared across sequences are stored and managed in a relational gt ODBC capable database This applies to the entire gt Sample and gt Sequence Data The advantage of this type of data management is not only easy integration into applications such as Excel Access or dBASE but effi cient searching and sorting capabilities A Query allows you to find all samples processed on a certain day created by a certain user and or with a certain name Storage with the Sequence All data describing the chromatographic treatment of a sample or data recorded during the analysis are stored in a gt Sequence This includes all p
62. ce Control Controlling the DX 80 lon Analyzer The following operating functions can be controlled from the DX 80 control panel Pump Turns the pump on and off During a running batch gt Batch Process ing is stopped Connect Disconnect Connects and disconnects the DX 80 from the gt Jimebase Autozero Resets the signal to zero Monitoring the DX 80 lon Analyzer The following operating functions can be monitored but not controlled from the DX 80 control panel Function Meaning System Status Checking Indicates that the signal is being checked to ensure that Ready conditions are met before the sample is loaded Ready Indicates that the system passed the check and is ready to load the sample Running Indicates that data acquisition is in progress AS40 Indicates whether the AS40 Automated Sampler if installed is idle or loading Valve Indicates whether the injection valve is in the load or inject po sition Manual Over Manually toggles the injection valve between the load and in ride ject positions Signal The offset or total signal value Autozero Resets the signal to zero Show Plot Displays the signal window Pump The pressure reading Program Time _ The elapsed time and the total run time Audit Trail The gt Audit Trail How to Using Keyboard Shortcuts 115 Many operations especially in online control can be performed via shortcuts General Edit Control Signal window Comm
63. ceaseeeseaeeessaeeessaaes 62 E Peak Summary HRepnort 62 E Chromatogram Compartson leg e EE i GC eects sera E E Actions in the Server Configuration Program scccsssseesseseeeeeees E Changing the Server Configuration sesnsssnreerrrerrreerrrerrreen E Selecting the Copy Protection Location and Entering the Key Code E Updating Moduleware oesnonnnoennesrnneerrrsnrrerrrrerrrerrrrerrrerrrerrreene E Adding Timebases Instrument ccccceeeceeeeeeeeeeaeeeeeeaees E Enabling and Disabling System Wellness Functions Actions in the Control HEEN 76 E Loading a Control Panel 76 E Connecting a Control Panel with a Timebase soeeeennenernean LL H Using Recording Demo Data ACTIONS IN EMG ESO WS GE EE Creating a Sample List Sequence Table cccccsseeesseeeeeeeees How to Setting Up a DataSOUrce ceccceeeeeceeeeeeeeeeeeeeeeeeeeeenes 81 E Connecting a Databaee cccccccccccseceeceseeeeeeeeteeeeteneeeneneeens 82 B Disconnecting a Database n0annnnannnnennnnnannenrnrnnnnnrnrenrrnenni ER H Creating a New Datoabase ic cccecccceecccceeeeeeeeeteeeetaneeeneneeens ER E Opening the ODBC Manager Handling Files Datasources and the Network ccsseeee 84 E Locking Datasources on Removable Mecda 85 E Locking Datasources Directories and Sequences 5 DA E Opening Protocol Data Audit
64. ces in a datasource A sequence is represented by a blue file folder It is not possible to create a subdirectory below the sequence level Operation e Select a datasource any subdirectory or a sequence to view its contents in the right hand window section of the Browser In the case of the datasource or subdirectory you see the usual list of all di rectories and files If you select a sequence the right hand window half is di vided into two horizontal sections These include the header with general Basic Operation 30 properties of the selected sequence and a list of all analysis and standard samples and their sample data Sequence Editor Caution The sample data can be edited directly here Additional sam ples can be included in the sequence existing sample data can be modified or old samples can be removed For a de tailed description of the sequence editor refer to the Se quences section e Press the right mouse button and choose the Query command to search for specific sequences data or samples also simultaneously in several data sources This is a true gt Query that can also search for specific properties Moving deleting or copying directories data and files is very similar to the corresponding operations in the Windows Explorer see gt Common Features with the Windows Explorer Caution Moving deleting or opening chromatographic files must be performed exclusively in the Browser as process
65. cssueessecsseeesseesseesseesseensieen 28 Data e EE ee BR dee Le TE MG OO E Contents Raw Data Storage cccccccscecesseeeeeeeeeeeseneeeeeeeeaeeeeneseeseeeeneseeeseneeeaees Raw Data Compression ER Restoring a Chromatogram from Raw Data Raw Data XO ON ensexasieeeverdadewsicenmeurvssesurestecdanermsernuasvasdsasieioescumvsccansuaed Raw Data BND OI aie vincencatansrnerrenescinanwasoensecsuseuntasinerausabenneetavanceacsiinaeisas Raw Data Storage in Power Off Case ccccesseeeeseseeseneeeeeeeseees Protocol Data Audit Tree ebe ebe ees H Daily Protocol E ES BB Sample PLO CO EEN Samples and Sequences cccceeeceeeeeeeeeeeeeeseneeenesenesenesenesoneeeneseaeess Sample Preparation eege ees ege ed eg Sample Processing WE Sample Registration EG Sample List Sequence Table cccccsseeeseseeeeseeeeeneeseneeseenenees Sequence Wizard WE Kalabrien Automatic Batch Operation Online Batch Sample Evaluation BE E He Ed a TN EE EE UE ACI ON EEN H Installation Qualification Manager 55 H Log File CMSETUP LOG ae System Wellness Toa a a System Wellness OVErview cccscceseceeeeeeeeeeeecenesenesenesenesenesenesens BI ge Le Leila EE Definition Data Repnrocessing kee EERR KEREN KEREN RE ERKENNEN iv Contents Ire EE Overview Integration kSeeREERRKE REENEN ERR EERR ERKENNEN ERKENNEN DU EESE E EEN DI H Integration Report ccccccccccsssccccessseeceessees
66. ctivities The user selects the sample or sequence to process and automatically re ceives the corresponding view e g integration samples are displayed in the integration window and sequences are displayed in the sample editor For each sample or sequence only the selectable or expected views methods or functions are available to the user This saves time and reduces the number of simultaneously active windows This context sensitive procedure is also valid for options selectable with the right mouse button The selection depends on the position and the time of the mouse click Glossary A 29 Sample Type The gt Sample Variable Type specifies which type of sample is used Unknown indicates an unknown analysis sample This sample type is indi cated by the symbol Gi Standard indicates a standard sample with a known concentration This sam ple type is indicated by the symbol D Sample Variables Overview The variables in the 5 Sample List Sequence Table columns are referred to as sample variables They characterize a sample and are stored in a database as part of the gt Sample Data The following sample variables are available Columns that can be edited Je Comment gt Sample Name Name gt Dilution Factor Columns that cannot be edited Compound names Nr Sample Number Sample Type Type Sampling Rate The number of stored signal values per second is referred to as sampling rate or data collection rate
67. d e Integration commands for processing chromatograms k O We a f e Status Bar enables disables the Status Bar Move the mouse pointer over an icon to view its name in the quick info dis play Trigger Commands A trigger refers to the automatic execution of a command as soon as a condi tion becomes true For example a trigger command Sound can be used to Glossary A 37 generate acoustic signals When specific conditions are fulfilled the acoustic Signal is issued A Trigger command can be included in a programmable button but not in the online control The following syntax is valid Time Trigger TRIGGERNAME Condition True Delay Limit Hysteresis Reaction 1 Reaction 2 Reaction Time EndTrigger The activation time and the trigger name are selected by the user If the condi tion specified via Condition and True becomes true all commands between Trigger and EndTrigger are executed Execution is delayed by the Delay parameter and can be limited to a specific number of executions via Limit As several triggers can be simultaneously active all trigger commands must have unique names Terminating an active trigger condition is by entering the trigger command again with an added zero and closing it without a reaction Time Trigger TRIGGERNAME 0 EndTrigger This applies to each single trigger It is not possible to simultaneously close all triggers The times at which a trigger is actuated are entered in the A
68. d displayed in relation to the new Zero point Ke Notes The Acquisition On command automatically performs the Autozero function A 6 Glossary Backup The Backup command allows storing data for data security The procedure is described in the section How to Actions in the Browser Creating Backup Files The Dionex Data System stores data in databases and other files organized in a hierarchical directory structure There are many links among the included objects For problem oriented data archiving it is necessary to know how individual objects are linked with each other Because conventional backup and archiving programs do not offer this support the Dionex Data System has its own backup program This integrated backup program offers the following functions e e Archiving of all objects displayed in the Browser samples sequences files entire directory trees etc together with all linked files gt Raw Data Audit Trail Al Protocol Data history preferred RDF file etc e Selective restoration of objects stored via backup e e Compression of the stored data e Division of the backup files onto several media e g diskettes or ZIP drives e e Optional deletion of original files after the successful backup e Generation of corresponding entries in the file history Backup files are located outside the datasources Therefore backup files can not be stored within the directory structure of the datasources
69. d via the gt Hold and gt Stop commands The gt Continue command is used to resume acquisition To terminate raw data acquisition select the Acquisition off command B Notes To reduce the raw data storage requirements Acq on should be activated after the Inject command but at least 30 seconds before the elution of the first peak The command can be selected from the Menu command or automatically via the gt PGM File AIA Analytical Instrument Association AIA is the abbreviation for Analytical Instrument Association an association comprised of companies that manufacture and sell chemical analysis instru ments and software The AIA is dedicated to developing guidelines for a stan dardized chromatography file format a standardized format facilitates data ex change between laboratory systems produced by different manufacturers Standardization efforts also involve operation documentation maintenance and GLP Good Laboratory Practice requirements The generally valid AIA export format for chromatography data is known as ANDI ANalytical Data Interchange Chromatography Since 1992 there have been five data format categories Category 1 through 5 However only Cate gories 1 and 2 are widely accepted Data formats of Category 1 comply with standard raw data unit and scaling guidelines and include information about where the original data and corre sponding methods are stored This allows exact restoration of a chromato
70. data All data from a sequence are stored in the cmb format system backup cmb ASCII Raw data are exported in the ASCII format Use the Batch Report com mand on the File or context menu Click Export in the dialog box and then select the ASCII export format All raw data for the actual sequence are stored in the ASCII format It is also possible to export data sheets of a report in different formats see Al Data Export Raw Data Import It is possible to import the following raw data formats e gt AIA data txt e Dionex data system archive cmb From the File menu select the Import Restore command and then the corre sponding command Use gt Restore to import Dionex Data System backup data Data 47 Raw Data Storage in Power Off Case The raw data of a sample interrupted by a power failure are not lost as raw data are automatically saved autosaved continuously during sample proc essing The autosave ensures that raw data are stored on the hard disk in short intervals The user can reduce the time interval to approximately 30 seconds In the case of a power failure the maximum data loss would thus be 30 sec onds If a power failure interrupts the automatic sample batch the gt Power Failure Protection and the Power Off Handling ensure that processing resumes at the same point when the system is restarted Protocol Data Audit Trail In addition to raw data the Dionex Data System also records th
71. dicular line dropped to the baseline PT pretangent skim The peak is interpreted as a RuorRd rider A 12 Glossary Device Driver Each controllable instrument has its own device driver The driver is activated when the Dionex Data System is started The device driver translates the user s PC instructions via mouse click into device specific digital commands that are converted into real control commands after the data are transferred to the instrument Similarly the signals of all instruments readings status in formation etc are converted so that they can be read by the Dionex Data System and can be displayed on the screen Device drivers are also capable of monitoring regularly inquired or automati cally given device parameters in a target actual value comparison Unexpected events can be corrected or displayed in an error message If the functionality of the driver can be modified via parameters this is performed in the Al Server Configuration Program The names of the device drivers are derived from the corresponding instru ment The extension CDD means Chromatographic Device Driver The de vice driver for the DX 80 lon Analyzer is DX_DX80 CDD During the Dionex Data System installation all device drivers are saved in the BIN directory and receive a version ID that can be viewed via the Properties command on the context menu eg in the Explorer Dilution Factor The gt Sample Variable Dilution Factor is a
72. e For further details refer to the chapters Installation Software Protection and How to il Actions in the Server Configuration Program Selecting the Copy Protection Location and Entering the Key Code Drag amp Drop The Drag amp Drop function is a way to copy move and print any element in stead of selecting the corresponding menu commands The Drag amp Drop function is available in both the online and offline modules and is most useful when editing lists Press the mouse button and keep it pressed to move the selected element to its new position To move an object use the left mouse button To copy an object use the right mouse button F1 Key Press the F1 key to open context sensitive online Help this is the displayed help text that refers to the current cursor position A 14 Glossary File History The File History mode allows complete documentation of any changes and op erations performed in specific objects and files Objects include samples gt Se quences or datasources Control Panels report templates RDF gt PGM Files and or gt QNT Files and of course modified chromatograms The File History mode can be activated or deactivated individually for each datasource see Properties of a datasource Enable Modification History When this mode is activated each user is prompted to enter a comment before saving the changed object To view the changes documented so far select a data
73. e data system are referred to as physical signals The measuring data of the different signals is stored in uniform raw data files Signal Noise See gt Noise Signal to Noise Ratio The signal to noise ratio serves to characterize electronic components The lower the noise the smaller the possible signal variations that can be recorded As a general rule the average of a signal should be at least twice the standard deviation of the signal value Status Bar In the status bar on the lower window margin messages regarding the current system status are shown on the left and the current timebase and the currently executed command are shown on the right The display of the status bar can be enabled and disabled via the View menu Stop Flow The Stop Flow command switches off the pump flow and interrupts data ac quisition A running batch is stopped as in the hold mode The Stop Flow command is executed via the Control Stop Flow menu command Use the gt Continue command to undo this command Suppressor A suppressor is a patented electrolytic device from Dionex which reduces background signals and enhances analyte signals in conductivity detection Conductivity detection requires eluents that contain strong electrolytes These electrolytes can interfere with detection of the ionic analytes of interest The suppressor uses ion exchange to neutralize Suppress the electrolytes in the A 34 Glossary eluent thus reducing th
74. e Dionex Data System via the LAN local area network certain built in diagnostic and calibration functions are provided These functions known as System Wellness are enabled and disabled from the Server Configuration program See the following topic for details al Enabling Disabling System Wellness Functions Changing the Server Configuration Starting the Server Configuration Program To start the Server Configuration program select gt Server Configuration from the Start Programs menu on the taskbar i e select Start Programs Peak Net 6 Server Configuration Caution Modifications to the server configuration are only possible if the corresponding gt Server was started before Normally this is done automatically when starting the computer on which the server is installed 70 How to Configuring the Server Select the name of the server on which you want to extend or change the con figuration and double click Then enter the gt Key Code and the type of Software Protection Adding Components Select the name of the server of which you want to extend the configuration Click the character in front of the server name to view its current configura tion Select the following commands via the Edit menu or the right mouse button e Add Timebase to install a gt Timebase on the server Enter the name of the timebase in the Edit box e Add Device to add more chromatography instruments to a timebase Se
75. e ODBC driver Select the Always use native SQL option if you want to edit the SQL statement manually AN Caution If SQL conditions were edited this translated SQL state ment will be used to perform the query e Result Restrictions tab The list of samples found via the SQL query can be further limited by result restrictions Open the Edit Conditions dialog box by double clicking the left mouse button or via the context menu and then edit the result conditions Specifying result restrictions generally limits the query to samples that contain raw data e As soon as the search conditions have been completed you can start the search by clicking the Apply button Editing SQL Statements The Native SQL tab dialog box shows the SQL statement the Dionex Data System created from the database query specified via the SQL tab dialog box Users who are knowledgeable about the SQL database language can specify further conditions for their gt Query Be sure to select the Always use native SQL option if you want to edit the SQL statement manually Caution If you edit the SQL statement via the Native SQL tab this translated SQL statement will be executed whenever the query is performed as long as this box is checked How to 97 Examples Dialog Box The following SQL query searches for all samples of the current day Edit Condition K Sample Inject Time e during the previous e D Dayts m The SQL sheet will show
76. e background conductivity In addition the ion ex change mechanism replaces a less conductive counterion with one that is highly conductive hydrogen in anion exchange and hydroxide in cation ex change Since both the analyte and the counterion produce the detector re sponse the analyte signals are increased The anion version of the DX 80 lon Analyzer contains an AMMS Ill The cation version of the DX 80 contains a CMMS Ill For more information refer to the suppressor manual Glossary A 35 System Check PeakNet IA monitors the status of certain system parameters If a parameter does not meet specification the software displays an error message All error messages are logged in the gt Audit Trail Parameter Specification Comments Total Back lt 35 uS The total background is checked for up to 10 ground anion minutes before each sample injection While mode the system is equilibrating the Ready LED on lt 3 uS the DX 80 front panel flashes When the back cation ground meets the specification for 30 consecu mode tive seconds the Ready LED remains on con tinuously If the background fails to meet the specification within 10 minutes the Ready LED flashes The default severity for the error mes sage is abort Drift 100 uS lt x The first and last points during data acquisition lt 100 uS are used to calculate the difference Noise lt 10nS The final minute of data during data acquisition is used to calculate th
77. e data of the Al Audit Trail protocol data storage The following information is included e Commands from a control file e Manually executed commands e Commands activated by a trigger condition e Error messages e Start of sample processing e New start of the Dionex Data System after booting the computer Each item is stored with the current time Furthermore the current program time is added to information whenever data are recorded or a sample batch is processed Storage is continuous over an entire workday as well as for the duration of processing of a single file Therefore the following distinction is made Al Daily Protocol n Sample Protocol Audit Trails can be displayed from the Browser as daily or sample protocols or as Audit Trail control in the control panel To select the extent and type of the display a display filter Normal Advanced Expert and Error or Warn ing can be chosen from the context menu right mouse click Data 48 Daily Protocol The daily protocol stores all GLP relevant data related to the status of a spe cific timebase This information is displayed in the Audit Trail Control of the control panel When starting the Dionex Data System gt Server the AUDIT directory is cre ated in the server datasource below the timebase The server saves the daily protocol in this directory The daily protocol is given the current date as the file name e g 20000926 slg for the daily
78. e difference Template Templates facilitate entering data The Dionex Data System offers various pre defined templates the report template is DEFAULT RDF Time The Dionex Data System stores time stamps as universal time Greenwich time However the date notation is displayed according to the local settings chosen in the Windows Control Panel A 36 Glossary Timebase All components combined in a chromatography system to enable the chroma tographic separation as long as the components are related in a time context with each other are assigned to the same timebase A timebase consists of one DX 80 lon Analyzer with or without an AS40 Auto mated Sampler Only a further system that is completely independent repre sents a new timebase Administration of different timebases may be on one or several gt Chromatography Servers The name for a timebase is assigned in the Server Configuration program Toolbars Frequently used commands and features are available as icons Related func tions are combined on toolbars located directly below the menu bar When starting the Dionex Data System the standard toolbar is displayed Select the Toolbars command from the View menu to enable or disable the display of in dividual toolbars The following toolbars are available Standard standard functions e Online commands for the gt Control Panel e m Fee e Method for changing to other method sections or samples LE Ee
79. e layout of indi vidual peaks and the color shape and size of their captions Opening Additional Window Sections Use the following commands to display further window sections e Select the Split Zoom command from the View menu to split the window By drawing a frame you can zoom a section of the window The upper half of the window shows the entire chromatogram and the frame the lower half displays the zoomed section Use the mouse to move and position the frame in the upper window section This mode is recognized by the shape of the mouse cursor bt Of course a new frame can be drawn at any time Integration 62 e Select the Report command from the View menu to display the integration report see H Integration Integration Report e Compare two chromatograms by enabling the display of an additional chromatogram see Al Integration Chromatogram Comparison E Integration Report The integration report is opened via the Report command on the View menu It is displayed as a table in the window of the partial method Integration Contents e The table contains numeric data relating to retention time peak area and height amount peak type and other peak variables e f the chromatogram is modified this is reflected immediately by the changed report data e Via the Windows Clipboard data from the report can be directly integrated into other Windows applications e The chromatogram and the integration report can be printed a
80. e timebase assigned to the panel 4 If an error message appears stating that the timebase was not found close the message and then select Connect to Timebase from the Control menu Select the timebase to be connected to the panel 5 After communication is established with the timebase the calibration and diagnostic controls on the panel are enabled How to 101 Viewing and Restoring Calibration Data Calibration data for the DX 80 lon Analyzer is displayed on the Wellness con trol panel see How to al Opening a Wellness Control Panel The Dionex Data System stores three sets of calibration data for the DX 80 lon Analyzer e Current is the data most recently sent uploaded from the DX 80 It is the data currently stored in the DX 80 memory e Previous is the data from the previous time calibration data was uploaded from the DX 80 e Factory is the data obtained uploaded from the DX 80 when it was ini tially configured in the System Configuration program see How to n Enabling and Disabling System Wellness Functions The Wellness control panel displays the current data and the date the current calibration function was performed If the factory value is the current data the date field displays e To view previous or factory data or to download calibration data from the Dionex Data System to the DX 80 click the Details button A Calibration Details dialog box appears that displays the three sets of cali
81. each controlled device This is done automatically when the data system is started The device driver determines the instrument s scope of function The driver is responsible for all device specific tasks such as sending commands in the command language of the device or deciphering the data sent by the device Thus the control program of the data system does not have to deal with spe cifics but prompts the driver to execute certain instrument functions All drivers are stored in the BIN directory The version number assigned to a driver can be viewed in the Explorer via the Properties context menu Audit Trail The Dionex Data System device drivers send the parameters specified in the control file to the corresponding devices In addition they constantly monitor the device parameters and compare the nominal values with the actual values Deviations from the nominal values are either corrected or indicated by an error message Each command each correction and each error message is not only recorded and stored by the Dionex Data System with split second accuracy but is dis played in the Audit Trail window of the Al Control Panel For further information on storing protocol data refer to the following topic in the chapter Data Al Protocol Data Audit Trail Installation Software 15 Installation Software Tips for Software Installation For information about installation of the Dionex Data System refer to Al Initia
82. ees Device DiI Ver oeit Database ccceseeeeeeeeeeeee A 10 A 11 Bk c EE Connect DA GU ee ee Create General Commandes Data Storgage EE D acnnnnsisininnsa DataSOUICe ccccccccccccceccccencccce System Commande Disconnect Index iii DataSOUrce ccceccesccecceccescceccescescess DataBase sccccsccasecuaciavedecevesicuadiaceseaves Connect Uumebase CTE ANE s wsinconcsrincssoussiantieanecionansnen esses IEN IEN OleT Dongle irectesxscezzsteeztcienss Disconnect sceeeeeeeeeeeceeeeeeeeeeees AGT Oo vecececorenceesereceaeiecbonrteless Download cic E Calibration Data ODBC Manager eestetrsdegesteegsuegg Drag RE Removable Media IR E EH 81 Driver IR Device Dy Ci seicottcetsisadacantnssntconst DE ee 11 Device Drivers Overview Decimal Minute Delay E Trigger Commands Trigger Delay Saisadieetohseesannntewnae Error Message Delimiter Tool Audit Trail DELTA sugeari n Bxscel Demo SC EE Explorer vc cecccecccecceccccenceece Dein Modessa Export Dier Linearity 2rcccaesevdsnidcaxsieeccarzccvcd Det Noise and Doft F Det Wavelength eee Detection Code at Peak End Pl E Detection Code at Peak Start e TEEN Detector Create SEMSILIVILY En Handling Device Communication cces 6 OR Si ee Device Driver neuen Bestoe Dilution Factor sssssss File Histor Sample Vanahle nn E e EEN Dionex Data System un Lt Client User Interf
83. ell inlet Verify that there is a minimum of 7 MPa 1000 psi of backpressure Pump 1 0 mM KCI through the cell Allow the conductivity to stabilize for 5 10 minutes 10 Click the calibrate slope command button under conductivity cell The cell is calibrated and a new cell calibration constant is uploaded to the Dionex Data System The Dionex Data System stores this value as the cur rent cell calibration constant After calibration the conductivity reading should be 147 00 2 uS cm and the cell calibration constant should be between 130 and 190 If this is not the case refer to the troubleshooting section of the DX 80 operator s man ual 11 Flush the KCI solution from the system by pumping deionized water through the cell When the conductivity drops to near zero stop the pump flow 12 Reconnect the pump to the injection valve and reconnect the line from the suppressor to the cell inlet 104 How to Actions in the Window Section Chromatogram This window shows the integrated chromatogram of a sample Normally the software automatically determines and integrates peak areas However the user may manually modify the peak delimiters and baseline assign peaks manually and display other chromatograms for comparison For details refer to the following topics Manual Re Integration Manual Peak Assignment al Performing a Chromatogram Comparison For details on how to display a gt Report refer to the following
84. eplot when the signal leaves the right hand border of the signal window The current chroma togram is then replotted from the beginning The time axis is scaled by one unit so as to fit the chromatogram into the window For example if the first time axis is 2 minutes after 2 minutes the window is enlarged by 2 minutes to show 4 minutes after 4 minutes the window is en larged by another 2 minutes to show 6 minutes and so on Report Definition Report Definition Files RDF Files have two functions They serve to display and reprocess your results on the screen Report and to prepare the printout Printer Layout All settings regarding user specific representation of chromatographic data in different windows are stored in the Report Template Report Definition In conjunction with the gt Workspace this enables the user to arrange the screen according to personal preference If no workspace is loaded each new window is opened on the basis of the most recently used RDF File If no Report Definitions were stored the default RDF File included in the Dionex Data System DEFAULT RDF is used It is located in the Report directory Each RDF File contains specific information on window sections and views window presentations and captions color size of individual elements and the presentation of reports This requires the user to make decisions about the fol lowing features e Are the window contents e g the chromatogram s
85. equence in the left window section of the Browser e Enter the name and the properties of the standard and unknown samples line by line in the lower partial window on the right Proceed as follows e Click the cell in the table and enter the new value or the name via the key board e Enter a name for each sample to analyze in the column gt Name e Input in the remaining columns gt Dilution Factor and Comment is op tional How to 81 e f you are not sure which values or names to enter select the cell and press the F8 key This opens an edit dialog box in which all valid values and options are listed Select the desired value The cell input is then automatically updated e Press the F1 key to view further information about individual columns e Press the F9 key to fill all subsequent cells of a column with the same in put e Save the sequence with a new name Save as e Check the sequence properties by clicking Alt Enter D Tip It is possible to edit an existing sequence However the cur rently running sample cannot be edited the corresponding line in the sample list is indicated by a different color Automatically e Select the New command on the File menu and select Sequence using Wizard e Click OK to start the Wizard e Follow the instructions in the Wizard to create a basic sequence e Press the F1 key for more help How to Setting Up a Datasource All steps connected with the X
86. er off handling The following options are available e Continue with the interrupted sample e Continue with the next sample e Reprocess the entire online batch e Abort the entire online batch e User defined selection of the four options PQ See gt Performance Qualification PQ Pressure Limits The Dionex Data System is capable of monitoring the pressure value provided by the pump When a pressure limit is exceeded the DX 80 lon Analyzer gt Moduleware reports this to the Dionex Data System The Data System then stops the flow displays an error message and stops the sample batch as necessary B Notes Exceeding the upper pressure limit can be due to a blocked column or a defective injection valve If the pressure is below the lower limit it is probably due to a leak in the flow system Program File PGM File The program file PGM File can be considered a schedule for the execution of different control commands The file contains all commands to be executed before during and after the sample analysis This enables complete automa A 24 Glossary tion of the analysis including routine procedures before and after the analysis e g column conditioning It is also possible to monitor parameters or limits via the PGM File The PGM File cannot be edited Protocol Command The Protocol command enables the user to log specific events in the Audit Trail The command syntax is as follows 0 000 Protocol Text
87. es Short Checks all files The report includes only errors and warnings Generates the system information of your Dionex Data System Saves the current system status Use this option to save the names and the versions of all files con tained in the Windows System directory and in the BIN directory of an operable Dionex Data System version If you performed this option once the saved system status can be used as the basis for the file check see Check Installation Ex tended above instead of the IQDATA list Log File CMSETUP LOG Complete information about the system and the operations performed by the Setup is logged in the CmSetup log file The file is first saved to the Windows installation root directory it is moved to the Dionex Data System main directory after successful completion of the Setup This file includes the following information who performed the installation when the installation was performed which files were copied and which files were replaced by other files with the same names The latter is especially im portant as it indicates which default reports or control panels were replaced System Wellness 58 System Wellness System Wellness Overview What is System Wellness system Wellness monitors the overall health of the chromatographic system It provides built in diagnostic and calibration features that help prevent un scheduled system shutdowns and ensure reliable operation Calibration and
88. es you will be prompted for the name of a gt Report Template or a worksheet The results of a sample are then printed in the defined form However report templates or worksheets only refer to the presentation of results The Print Setup and Page Setup settings are stored separately for each worksheet in a report template Integration Calibration Peak Analysis etc Select the desired settings for each worksheet separately e Select the Print Setup command on the File menu to determine the printer the paper size and the format portrait or landscape B Note The Print Setup settings selected here apply only to the re port definition file of the current sequence They do not affect the default settings for Windows Similarly changing the de fault printer paper size and format in Windows will not affect the settings selected here e Select the Page Setup command on the File menu to determine the ap pearance of the headers and footers the size of the margins the alignment etc You can also set a grid determine whether column headers are dis played and specify whether the printout is in black and white or in color e Select the Batch Setup command on the File menu to determine how to print or to export Specify which worksheets of a report template are printed How to 113 and for which type of samples this is performed Also define the export for mat and specify where to store the data to export 114 How to Devi
89. es below the surface are performed apart from the visible result Function e Use the Browser to set up gt Datasources and to create subdirectories to move files via Drag amp Drop or to delete files e Select a gt Sequence in the left window blue folder to display a list of its contents on the right e Double click a sample name to view the corresponding chromatogram e Select a file and press the right mouse button for further functions e Select several files as in the Windows Explorer and press the right mouse button to perform a function for several files simultaneously e Select a file press the right mouse button and start a gt Query for several sequences and or datasources The result of the query different files or samples with at least one common feature is displayed in the right hand window section of the Browser Basic Operation 31 Method Window Offline windows represent chromatographic results they show chromatograms and lists of results General Method windows can be opened via any of the following e The View menu e Toolbar icons e Double clicking a file The appearance of method windows can vary considerably A window can have various window sections whose borders can be moved If the window section is too small to show all information the hidden area can be viewed via the scroll arrows or bars The contents of the window section depend on the type of data represented A table editor
90. es of one sequence A Summary can also be created on the basis of a gt Query For details see Al Peak Summary Report Glossary A 21 Performance Qualification PQ As defined in cooperation with EURACHEM Performance Qualification is the process of demonstrating that an instrument consistently performs according to a specification appropriate for its routine use P Bedson and M Sargent Ac cred Qual Assur 1996 1 265 274 The task of PQ is to prove and to document that your system functions as re quired by your special routine operation The frequency and the scope of the Performance Qualification depend on the special requirements in your labora tory This can be specified in internal instructions or in an SOP The parame ters should be similar to those of routine operation In addition to the Perform ance Qualification the gt Operational Qualification is an important factor for validating instruments and software The Qualification menu of the Dionex Data System Browser includes the In struments PQ and gt PQ Setup commands Select Instruments PQ to perform the performance qualification Select PQ Setup to generate the templates re quired for performing the performance qualification This is usually necessary only after a new installation or when the configuration was modified The Dionex Data System CD supplies a datasource with a master template in the directory named PQ Templates PQ This template is desi
91. et Activate the Time check box to move the current chromatogram in the x direction Activate the Signal check box to move the current chromatogram in the y direction The offset is specified as a percentage of the signal or time axis A signal offset of 5 shifts each following chromatogram upward by 5 Accordingly a time offset causes a percentage alteration in the x direction This results in a pseudo 3D presentation 110 How to Actions in the Window Section Report The Dionex Data System offers numerous options for working with gt Reports For details refer to the topics below Al Displaying a Report X Peak Summary Audit Trail Displaying a Report Each of the method windows integration or calibration plot can include a nu merical gt Report e Select the Report command from the View menu If you have not generated a report before a default report will be opened The default report contains several worksheets or report types Integration Cali bration Peak Analysis Summary and Audit Trail e Use the tab in the lower left corner of the report to change from one work sheet or report type to another Each worksheet includes a number of default variables that are an integral part of the corresponding report type For example in an integration report these include the Ret Time Area and Amount columns A calibration report con tains columns named Offset c0 Slope c1 Curve c2 etc
92. etc User Profiles Workspaces It is possible to save any combination of windows in a workspace This facili tates opening single files or windows and allows the user to work with the preferred work environment on the screen Information about which windows should be used is stored in a WSP File If you want to start working with a specific workspace open the corresponding WSP File or generate a new WSP File by storing the current screen contents e Select the Open Workspace command from the Workspace menu to open an existing workspace e Select the Save Workspace command to save the current workspace e Select the Save Workspace as command to save the current workspace with a new name The appearance of each individual window is stored in the al Report Defini tion There is no limit to the number of Al Windows that can be saved with each workspace B Notes When the Dionex Data System is started the most recently used workspace is loaded Increasing the number of dis played windows and data in a workspace will increase the loading time Basic Operation 33 Report Definition The gt Report Definition RDF File contains current settings such as the names and scaling of axes the representation of chromatograms display of additional information or auxiliary lines settings for various font types and sizes and the arrangement of columns in a table The report definition also determines how the current screen contents
93. gned for the Per formance Qualification in a standard HPLC configuration In addition to various sequences the template contains a PQ report template with the following sheets SPECIFICATION The SPECIFICATION sheet lets you enter the system specifications instru ments fluidics and limits INJ FLO _PREC_AN and INJ_FLO PREC CAT The IN ELO PREC_AN and INJ_ FLO_PREC_CAT sheets let you check the reproducibility of the injector and the retention time for an anion system and cation system respectively If the result is within the specified limits the Re sult column indicates OK INJ_CARRY_OVER_AN and INJ_CARRY_OVER_CAT The INJ CARRY_OVER_AN and INJ_ CARRY_OVER_CAT sheets supply a measure for the carryover in the anion system and cation system respectively If the result is within the specified limits the Result column indicates OK A 22 Glossary DET_LINEARITY_AN and DET_LINEARITY_CAT The DET_LINEARITY_AN and DET_LINEARITY_CAT sheets supply a meas ure for the linearity of detector response and peak height for the anion system and cation system respectively DET NOISE DRIET AN and DET_NOISE_DRIFT_CAT The DET_NOISE_DRIFT_AN and DET_NOISE_DRIFT_CAT sheets supply a measure for baseline noise and drift for the anion system and cation system respectively Audit Trail The Audit Trail sheet shows the protocol data Audit Trail for the analyzed sample several additional Data sheets are available for calculat
94. gram from raw data Data formats of Category 2 also transmit all calculated results e g on the ba sis of a chromatogram in addition to amount values and names for substance identification The data can thus be managed in a database or a LIMS system Using the AIA format ANDI in the Dionex Data System The ANDI format can be used to export data from the Dionex Data System Select the Export Backup command on the File menu of the Browser and A 4 Glossary open the corresponding dialog box via the ANDI Chromatography AIA op tion It is also possible to export and print data simultaneously Select the Batch Report command on the File menu of the Browser The Export option opens the Batch Setup dialog box The AIA format is defined on the AIA format cdf tab You can also import AIA files into the Dionex Data System Select the Im port Restore command on the File menu and select ANDI Chromatography AIA A dialog box opens in which you may select the file you want to import AIA Peak Type See gt Detection Code at Peak Start End Audit Trail An Audit Trail is a daily and sample protocol that is kept independently by the system The Audit Trail contains all operations relevant to GLP Good Labora tory Practice such as current and future commands events and error mes sages For additional information refer to the topics Al Audit Trail H Protocol Data Auto Autoscale Use the Auto Autoscale option to a
95. he CTRL key to also display the chromatogram of the next sample e Click the Previous Chromatogram icon BI while pressing the CTRL key to also display the chromatogram of the previous sample Integration 65 For details on selecting chromatograms to compare see How to Al Actions in the Window Section Chromatogram Performing a Chro matogram Comparison Placing chromatograms in relation to each other In general the appearance of a chromatogram is affected considerably by such factors as solvent column quality etc As these conditions cannot be modified later other possibilities must be found to perform as exact a comparison as possible This is achieved by adapting the position and the size of a chroma togram to match another There are several ways to achieve this e Chromatograms can be assigned an offset in the x and y direction e Chromatograms can be time normalized i e they can be overlaid at a spe cific retention time e Peak height can be normalized i e the height of a specific peak can be adjusted e Chromatograms can be stretched and compressed e Chromatograms can be subtracted from each other For the active chromatogram Decoration is chosen via the context menu Enter the settings in the Comparison tab dialog box For further details see How to H Actions in the Window Section Chro matogram Performing a Chromatogram Comparison Printer Layout 66 Printer Layout EI Printing
96. he Server Configuration program Starting the Server Configuration Program e To start the Server Configuration program and modify the server configu ration select Server Configuration from the Start Programs menu on the Windows taskbar i e select Start Programs PeakNet 6 Server Configu ration Caution Modifications of the installation are only possible if the server to be configured is started see the following topic in the chapter Installation al Program Start and if no sequences or samples are currently processed on this server When opening the Server Configuration program for the first time or when changing an existing server configuration there are also changes in the direc tory structure Description The user interface of the Server Configuration program contains two partial windows The left hand window lists the names of all currently active started chromatography servers The right hand window logs the configuration process Audit Trail A running server is shown in the shape of a PC icon an inactive server is crossed out red An active server for example one busy processing samples is indicated by a red prohibited sign red circle with white line Click the character next to any server name to see its timebases and hard ware components Timebase Hardware In the same way the chromatogra phy instruments assigned to a timebase Devices can be displayed Informa tion on the type and number of all
97. herboard with a built in LAN card Hardware and Software Chromatography Components 7 Timebases If you intend to operate a single chromatographic system with the Dionex Data System you will work with a single timebase All instruments are time dependent on each other and are synchronized during operation i e they have a common clock If you control several systems the individual systems work independently asynchronously from each other the Dionex Data System must manage a separate timebase for each system i e the systems behave as if they were controlled by separate PCs PC These are the guidelines for personal computer PC specifications for the Dionex Data System e IBM compatible computer Intel Celeron 400 MHz minimum e 32 bit Al Operating System Microsoft Windows 98 Windows NT 4 0 or Windows 2000 e 64 MB RAM minimum e Hard disk drive with minimum of 2 Gbyte free space e 10BASE T compatible PCI LAN card e CD ROM drive e Windows compatible monitor resolution of 1024 x 768 pixels minimum e Mouse supported by Windows Hardware and Software Chromatography Components 8 Operating System The Dionex Data System supports the following operating systems e Windows 98 e Windows NT 4 0 e Windows 2000 Because these are 32 bit gt Multi Tasking operating systems several programs can be executed simultaneously This is especially important for instrument control data acquisition and printer support
98. hown in the zoomed view or full scale view e Which type of axis scaling is used e Which additional objects grid comparison chromatogram units etc are displayed Glossary A 27 e Which colors are used for comparison chromatograms captions etc e Are other window sections displayed Calibration Curve Report etc e Which worksheet makes up the report and how is the report formatted e Which columns does the worksheet include Restore File The Restore command allows you to restore files previously stored via the gt Backup function The procedure is described in the section How to Al Actions in the Browser Restoring Backup Files To implement the Restore command select Import Restore on the Browser File menu B Note The easiest way to open these archive files which have the extension cmb is to double click them in the Windows Ex plorer Right Mouse Button The most common commands and functions are available via the right mouse button The choice is context sensitive i e the commands most likely to be used in the current situation are available RPC Remote Procedure Calls The RPC technology introduced with Windows 95 NT makes it possible to run a program on a different computer Unlike true full control programs such as PC DUO or PC ANYWHERE the amount of transferred data is considerably lower with this type of control Proc esses started from a remote computer run almost without any
99. icense tab of the Preferences dialog box allows you to select the copy protection location and to enter the key code as described below In the case of a server PC open the Server Configuration program by clicking Server Configuration Double click the appropriate server name to access the General tab The options described below are possible only if the corresponding Chromatography Server was previously started Generally this is performed automatically when starting the computer on which the server was installed If this is not the case start the Dionex Data system via the gt Monitor Program Selecting the Software Protection Location Specify whether your system is protected by a gt Dongle or a gt License Server Select Dongle if your system is protected by a dongle on the parallel PC interface Select License Server if your license was generated by a license server This disables the edit field for the key code Clicking the Browse button to the right of the License Server opens a dialog box where you spec ify the computer on which the license server of your Dionex Data System is running Entering the Key Code The key code is also entered via the Properties or License dialog box Enter the 12 digit key code in the corresponding field 74 How to Updating Moduleware Follow the steps below to install a new version of gt Moduleware in the DX 80 lon Analyzer 1 Insert the CD containing the new Moduleware versio
100. idual in struments and device functions via a i Control Panel Please note the fol lowing requirements e The data system must be extended by one of the X Dionex Data System Options e Correct installation and configuration of the controlled instrument s and de vice driver s via the Al Server Configuration Program e Establishment of an intact connection between the PC and the chromatog raphy server e Establishment of an intact connection between the chromatography server and the chromatography system via the Al LAN For an overview of the executable system and instrument commands refer to the following topic al System Commands Control 37 E System Commands system control comprises all commands relating to the entire chromatographic process or the entire system The commands are available via Command on the Control menu Some commands are available on the toolbar also Abort Sample Stops data acquisition and sample continues the gt Batch with the next sample Abort Batch Stops data acquisition and batch processing gt Stop Flow Stops the pump flow interrupts data acquisition stops batch process ing gt Hold Interrupts data acquisition holds batch processing gt Continue Continues all functions in hold mode Acquisition On Off Starts and terminates data acquisition DX 80 lon Analyzer Commands The following commands are available for direct control of the DX 80 lon Ana lyzer operati
101. ilar conditions are con sidered several samples Defining a sample means the process of determining how much of a substance is injected from which vial and under which conditions and which evaluation parameters are used A distinction is made between a single sample and a sample series Single Sample A sample can be analyzed individually by entering all required information and user commands via the keyboard or the mouse The user selects the Inject command enters the volume to inject and per forms the injection via a hand operated valve If an autosampler is available the user determines the sample location with the Inject command Then gt Data Acquisition is started via the Acquisition On command When the end of the sample is reached the user terminates data acquisition Acquisition Off command and specifies where to save the acquired data The recorded data are temporarily saved to the manual sequence of the de fault datasource of the system In network operation this datasource is desig nated with the computer name of the user in the network As soon as the user terminates data acquisition the user is prompted to select the final storage location for the temporarily saved data Sample Series If several samples are to be processed successively they are included in a sample list sequence table together with the instrument control and evalua tion information Then the samples are processed by starting automa
102. in Windows can be locked in the Dionex Data System Important data are often stored on central data server PCs If during data ac quisition the network connection is interrupted or the data server PC crashes data acquisition should of course be continued All data relevant for the Dionex Data System server are stored locally on the server s hard disk to en sure that data acquisition is not interrupted in case of a network failure In addition to allowing data exchange within a local area on a Windows Novell DEC or UNIX network LAN data transfer across huge distances WAN via ISDN is possible The basic requirement for any type of network operation is the availability of the corresponding network drivers for Windows 98 or Win dows NT 2000 Caution There are differences between the operation of the Dionex Data System under Windows 98 and Windows NT 2000 See the following topic in the chapter Installation Al Dionex Data System under Windows 98 and Windows NT 2000 Hardware and Software Chromatography Components 12 Overview Chromatography Data System The data system is the control center of the chromatography system The data system tasks are to Monitor the state of the connected chromatography instruments Log all user entries and modifications to the system Save and archive all data Graphically represent data and allow the user to check the system status and system results Allow the user to thoroughly chec
103. in this context are validation sample averaging normal distribu tion statistical and systematic errors blank reading selectivity etc The Dionex Data System offers numerous options for meeting GLP and valida tion requirements See the topics below for details AJ Installation Qualification Manager al Log File CMSetup log Validation 56 E Installation Qualification Manager The CMIQ EXE program located in the Dionex Data System IQ directory is referred to as the Installation Qualification Manager Following each Dionex Data System update this program automatically checks the current installation This check can be started manually as well Activating CMIQ EXE Automatically After copying all files and completing the Setup the program is automatically activated CMIQ EXE generates an ASCII report in a file named CHKSETUP log The file can be read using Windows Notepad or a word proc essor The file contains information on e The system time computer name user name operating system proces sor e The Dionex Data System version Setup date Setup operator key code serial number number of installed timebases status of installed software modules e All copied files file name and path file version date of last modification date file size in bytes product version 32 bit check sum The data are compared to the data in a write protected list IQDATA dat sup plied with the Setup If the lists are identica
104. ing further values Caution Do not edit report sheets even if editing is possible The Dionex Data System automatically reads the corresponding values Within the report individual data sheets are very of ten accessed via references If you insert or delete lines and columns these references will be lost Thus the calculations will be wrong The report must be printed as a Batch Report from the Browser to ensure that in the report the data are read in and processed correctly Select the se quence for which the report will be printed Make sure that no sample is se lected Select Batch Report on the File menu and start printing by clicking OK PGM File see gt Program File Power Failure Protection The Dionex Data System recognizes during the load sequence of the data system whether online gt Batch Processing was interrupted by a power failure etc This is possible because the date and the time are entered in the sequence editor whenever processing of a new sample is started The Dionex Data Sys tem checks whether all started samples labeled with date and time were completed i e whether they reached the status F for finished If this is not Glossary A 23 the case the gt Power Off Handling offers various options to continue process ing Power Off Handling The Power Failure Protection ensures that online batch processing contin ues even after a power failure This protection is determined via the Pow
105. installed components as well as their con figuration is stored in the file CMSERVER CFG Installation Software 21 Installation Configuration Procedure Access to a server and the ability to change its configuration is only possible after the server has been started and if sequence and sample processing are not in progress The Server Configuration program allows adding and removing timebases hardware and instrument components The configuration of each component can be changed here The changes are saved in the gt Server Configuration Select Connect Remote Computer on the Server menu to establish a connection to a specific server Enter the computer name of the server and the protocol to use As soon as there is a connection to the server its name and symbol appear in the left window of the Server Configuration program Select Add Timebase Add Device on the Edit menu to add new compo nents to the system Select a component in the file structure and double click to change its con figuration Or choose the Properties menu item via the right mouse button In both cases a dialog box containing several tab dialog boxes is opened these contain all configuration options When opening the Server Configuration program for the first time or changing an existing server configuration the directory structure also changes The Dionex Data System creates a local datasource under lt PCNAME_local gt For each timebase on the cu
106. int in the Browser the cur rent sample list with either the corresponding PGM File or QNT File Printing with Report Templates If one or more samples or sequences are selected in the Browser Batch Re port starts the printed output The user specifies the report template gt Report Definition to be used as well as which pages are printed for which sample type from the selected report template Printing in an Online Batch If several samples are processed in an automatic sample batch gt Batch Proc essing it is possible to define whether to output results after completing the process and how the results are output Printing can be started after processing a single sample or a complete se quence D Tip The report template used for printing is stored with other set tings e g screen settings in the Report Definition RDF File Control 36 Control Control Concept Controlling a system makes high demands on the adaptability of the chroma tography data system as operation of individual components must be easy and thus device independent That is input and representation of a specific con trol command is always performed in the same way The Dionex Data System enables this by the chromatography BIOS and AJ Device Drivers For details about control of the chromatography system see the topic below Al Control Direct Control Direct Direct control means the immediate and interactive operation of indiv
107. ion Creating a database is possible only if the selected directory is absolutely empty e f Common Data Source is activated the Dionex Data System gt Server can access this datasource Processing sequences is possible only on da tasources with this setting 84 How to D Tip The local datasource is always selected as Common Data Source If a datasource is not defined as Common Data Source under Windows NT Windows 2000 the datasource must be set up for each potential user Upon completing the procedure OK the Dionex Data System generates a database named cm_local mdb in the selected directory Databases with dif ferent formats cannot be generated in the Dionex Data System they must be created with the appropriate database software Opening the ODBC Manager Caution Modifications to the ODBC configuration should be performed only by experienced users Otherwise loss of data may result and re installation of the Dionex Data System may be necessary Normally no modifications are required here If a modification is required for example to repair a damaged database in a Microsoft Access datasource proceed as follows e Click the ODBC Manager button in the gt Datasource Manager to open the Windows gt ODBC dialog box of the control panel Handling Files Datasources and the Network To handle store and save data in the best possible way the Dionex Data system offers several options al Locking Datasources on
108. is the interval be tween the fulfillment of a gt Trigger condition and triggering a reaction If the parameter DELAY is used in combination with the parameter TRUE the command is only executed lt T RUE gt lt DELAY gt seconds after the condition is true Trigger Limit Normally a gt Trigger command is repeated any number of times each time the corresponding trigger condition is fulfilled This only changes if the trigger is terminated The parameter Limit enables you to define the maximum number of times the trigger is executed As soon as this number is reached the trigger automati cally ends A 40 Glossary E Trigger Hysteresis To prevent multiple trigger execution with very noisy signals each gt Trigger re ceives the Hysteresis parameter in addition to the True parameter In contrast to the True parameter hysteresis is signal dependent not time dependent The parameter can vary between 0 and 100 The default value is 5 ai Trigger Reaction As a response to a gt Trigger condition becoming true virtually any instruction can be performed Even trigger commands can be triggered TTL TTL Transistor Transistor Logic inputs and outputs are electronic switching devices for controlling instruments Unzoom The command undoes the last zoom operation In the signal window the Unzoom command can be implemented by double clicking in the gt Overview Window Wait The Wait command interrupts p
109. k and evaluate data Components The basis for precise control is a real time capable H Operating System Installation and configuration is the task of the Al Server Configuration Program Communication with different instruments and device types is enabled by the chromatography BIOS and the AJ Device Drivers Each operation execution of a command display of an error message etc is recorded in the 4 Audit Trail Depending on the required scope of performance various Dionex Data System 4 Options are available Dionex Data System User Interface The Dionex Data System follows Windows conventions regarding the menu structure screen elements and operation see Al Start Screen Hardware and Software Chromatography Components 13 Operation e Before the Program Start the correct hardware configuration must be ensured as well as the connections between the PC and the system and between the PC and a datasource e In Sl Network Operation the client PC can control systems and data sources that are not directly connected to the PC e Operation of the data system is facilitated by various assistants called gt Wizards Dionex Data System Options The Dionex Data System s scope of performance can be adjusted to the re quirements of a specific application In addition to the basic software package various options can be purchased The purchased scope of performance is coded in a copy protection chi
110. l Installation and Update The software installation procedure is also documented in the PeakNet IA System Installation Instructions Document No 031717 After installation refer to n Program Start Initial Installation and Update D Tip For additional information also refer to AJ Dionex Data Sys tem Under Windows 98 and Windows NT Windows 2000 Dionex Data System Setup Initial installation of the Dionex Data System is started by opening the Setup program SETUP EXE If CD Autostart is active on your PC the installation is started automatically when a CD is inserted into the drive Caution The gt Server the gt Client and the server monitor must not be active during the Setup procedure Make sure that there are no minimized program buttons on the Windows taskbar and that there is no server monitor icon Follow the installation steps to correctly install the Dionex Data System Please take note of the directory name in which the Dionex Data System is installed At the end of the installation process a program group lt NAME of DIREC TORY gt is generated the program group includes five links to start the different program parts and to uninstall the Dionex Data System The programs on which the links are based are located in the BIN directory in the directory structure created by the Dionex Data System Installation Software 16 The DATA directory contains various subdirectories PANELS REPORTS PROGRAMS P
111. l successful Dionex Data System installation is confirmed As soon as there are differences the Dionex Data System issues a warning or error If a warning is displayed the functionality of the Dionex Data System should not be affected You can start the Dionex Data System as usual If any problems occur please contact Dionex Service The same applies if the Dionex Data System reports an error Activating CMIQ EXE Manually Especially after installing or updating other software packages specific system files DLL used by several programs may be overwritten If this occurs it may impair the functionality of the Dionex Data System In this case it can be useful to open the program outside the Setup routine via the Installation Qualification command on the Qualification menu B Note For a correct system check of all files you must close all ap Validation 57 plications not just Dionex Data System modules Do not forget to close the Dionex Data System gt Server and the gt Monitor Program There are three options Check Installation System Information Save System Status Checks the files of the current Dionex Data System version and the required Windows system files The type and scope are freely se lectable Normal Checks the Setup Extended Compares the installation with the stored status see Save System Status below The scope of the report is freely selectable Full Checks and reports all fil
112. lect the instrument from the list Only one instrument can be added at a time D Tip When the timebase is created a corresponding folder is also created in the Browser This folder has the same name as the timebase and contains a default Sequence table and a folder for i Protocol Data files for the timebase Configuring Components Click the component to configure and select the item Properties from the right mouse button menu Each configurable component has one or more tab dialog boxes for alternative settings Refer to context sensitive online Help for infor mation on possible settings Deleting Components Click the component to delete and select the item Delete from the right mouse button menu iP Tip When deleting a timebase all instruments installed below this timebase are also deleted The same applies to deleting single hardware components or the complete hardware If you select a server and choose Delete the components and the installation file CMSERVER CFG are deleted How to 71 Copying or Moving Components Each selected component can be copied to the Clipboard via the Copy com mand and then inserted in a different place via Paste It is also possible to Cut single components Alternatively both actions can be performed via Drag amp Drop Saving the Server Configuration Each modification to the Server Configuration adding moving deleting or configuring single components can be saved There are two pos
113. ll found peaks are displayed in the Summary e lf the currently selected peak is a non identified peak e a peak not la beled with its name in the peak table a fixed time window 5 of the detected retention time is calculated for this peak All peaks within this time window will be included in the summary If two or more peaks are detected in the time window the peak that is nearest to the specified retention time will be selected If the peak is an identified peak this will be indicated by Ambiguous in the Summary header Creating a Summary e Double click one of the samples to open the Integration window e Display the Report and select the default Peak Summary worksheet which contains a selection of the most important default peak variables A sepa rate line in the report is reserved for each sample that is part of the Sum mary As in all reports the currently selected sample in the chromatogram window is highlighted in the Summary Table Chromatogram Comparison When comparing two or more samples or chromatograms a comparison of their numerical results would be the most exact method However overlaying Integration 64 the chromatograms is often sufficient This is performed via the chromatogram comparison What can be compared Multiple injections can be overlaid The number of simultaneously displayed chromatograms in the Dionex Data System is theoretically unlimited However when numerous chromatograms
114. ll samples that have raw data for this channel are compared How to 109 Relating channels to each other For a sensible comparison of different chromatograms a common reference point is generally chosen The chromatograms are normalized As the default the retention time is used for the normalization thus all chromatograms are ar ranged so that their start points match t 0 Alternatively chromatograms can use a common peak as their reference point Select the Decoration command on the context menu and open the Com parison tab dialog box Select the reference peak from the At Peak list box Select the Off option to overlay the peak selected below At Peak in all chromatograms Select the Shift option if this is to be performed independently from the length of the chromatogram Select the Stretch option if the chromatograms are to be stretched or com pressed in x direction so that there is a time scaling in all chromatograms The stretch O to 1 min or n min has the same length for all chroma tograms Activate the Normalize Signal check box if the peak height should match Depending on how much the reference peak must be stretched or com pressed all other peaks in the chromatogram are also stretched or com pressed This enables an estimation of the amount for the same peaks from different chromatograms lf you want to offset the chromatograms in x and or y direction this is per formed via the signal or time offs
115. mended as this would delete all Dionex Data System entries in the Registry e g key code and data sources Before updating the version close the server the client the Server Monitor Program and all other applications If the Setup program detects old PAN control panel or RDF report defini tion files in the PANELS or REPORT directories the user is prompted whether to overwrite existing destination files Therefore it is highly recommended that you save any personal PAN Files and RDF Files to prevent them from being overwrittten B Note Before performing an update copy all PAN Files and RDF Files you created to a separate directory to prevent the files from being inadvertently overwriten The Dionex Data System Under Windows 98 and Win dows NT 2000 The Dionex Data System supports the Windows 98 and Windows NT Windows 2000 operating systems Although Windows NT Windows 2000 and Windows 98 have similar user interfaces their cores differ considerably Windows NT re sembles large operating systems such as UNIX or VMS while Windows 98 still contains traces of DOS The main focus of Windows NT Windows 2000 is Stability safety and security These properties are responsible for the higher storage capacity demands of Windows NT and Windows 2000 When operating the Dionex Data System under Windows NT or Windows 2000 please note the following considerations Setup Performance of the Dionex Data System Setup on a Windo
116. mment will be opened Backup files have the extension cmb For information about how to open and restore backup files see the topic below Restoring Backup Files Backup files created via the gt Backup function can be opened via the Import gt Restore command on the File menu Also see How to Creating Backup Files B Note The easiest way to open archive files is to double click the files in the Windows Explorer Archive files have the exten sion cmb After opening the dialog box select the backup file to be restored The Dionex Data System recognizes whether the file is stored on a removable medium a hard disk or a network If the backup file is stored on removable media insert the first medium which includes a list of the contents The Dionex Data System displays the contents list of the backup file For a description of the individual fields see gt Restore Following this dialog box the selected objects are restored together with the raw data files of the Audit Trails and the history A new dialog box similar to the Backup dialog box appears If the backup is located on several removable media the user is prompted to insert the next medium as necessary How to 89 If the directory of an object to restore does not exist it will be created The cor responding sequence and datasource must exist Missing directories will be created as necessary If the destination object already exists the user is p
117. n Celeron is a trademark and Intel is a registered trademark of Intel Corporation Microsoft Windows 95 Windows 98 Windows 2000 and Windows NT are registered trademarks of Microsoft Corporation PRINTING HISTORY Revision 01 March 2001 Revision 02 April 2002 Contents Contents Jade liye tio EE Using the Online Help System and the Manual cscee Overview Online Herl eegegeeeeeeegeeeegieeee gege Activating Online Help skeERRRERRKE REENEN ENER EERR ERKENNEN REENEN Overview User Manual ccccccceeceeeeceeeeeeeeeneeeneseneeaneeeeeenesneseaes Theory Installation Operation ccccsseeeeeeeeeeees Hardware and Software Chromatography Components 00 Chromatography INStruMents cccceeseeeeeeeeeeeeeeeeeeeeeneseeeeseneeseees Device COMMUNICATION cccceecceeeeeeeeeeeeeeeeeeeeeeecneneneeneeeeeenesenennesaes Device Communication LAN cccscceeesssceeeceeeeseeeeeeseeseeneseneeneeens TiM OAS CS eect E a tennessee denen eee eee even eenence Operating System EE IOP EE 8 Local Client Server Installaton 10 Network JInstallaton Overview Chromatography Data System ccsceeseseeeeeeeeeeees 12 E Dionex Data System Options eaennnnnesennnnenennrneesnnrrrsenrrrreennn T3 H Device Drivers wesasvaiceccsisvsasenesnseinedsesasivsdveuasansenauuaiuedaeaaraudennsandence 14 le t UG Installation
118. n OnJOfT Connect with Timebase EE eebe erte E erleben eege EE Protocol Command IEN Eet Copy Protection Location Trigger Commands a000000 Correction Factor Undo Redo Command Sample Preparation 0000000000000 EE Create Communication 1 0 00 eeeeeeeeeesseeeeeeeeeeees SI E EE re Sequence Table Chromatogram Overlay CTRL ALT DEL Chromatograms Perform a Chromatogram D Compartson COMPTESSION egeeesdeeeesbe ege Steet Daily Protoeco Raw Data ocecerececteasasteocecrsecatnanictesss Data A 28 A 30 Computer ee Data Acquisition l CONGO Ms 2cnteremnnevelontionsdt Simulate oo cecececeescsesseeseeveeeeeees EE a satonctst arienspeenntanenenaiens Data Collection Rate Trigger Commands ssseeeseesees Data Compresseion Conductivity Detectpon Data Bvport Setiau in oteeanstoncestsnstspeetinteoxetsess Data TAP GS EE Year 200 irent ei Data LPO CODD CCU sosiaa Data Prec ceccceeecccesseceessseeeseeeees Connect to Timebase seen Data Reprocessing ccceeeeeeeees Connectivity Eege Data Security EEN 87 Open Database 0000000000000ean Data Storage 87 Bee Data Structure Online Help icsusssontornataadnnannienian Data System irscscesateesweceuieveeeacetecerectves Context Sensitive Help Component cccccceceseesseeseeeeees Continue FIOW EE EEN GOT UE geet Data Transfer cccccccccccccccecccccccccccceced EE Data Wave cococccccccccccccccccccccccececccce
119. n five different formats a ANDI Chromatography AIA cdf b ASCII text format txt c Excel file format xls d Windows metafile format wmf e Adobe Acrobat file format pdf f Dionex Data System archive format cmb In addition raw data can be exported in the AIA format from the Browser via the Export Backup command under ANDI Chromatography AIA Also see il Raw Data Export If you wish to send Dionex Data System data to another laboratory eg by e mail we recommend that you perform a AI Backup first and transmit the compressed data in the Dionex Data System archive format Data 44 Backup To avoid unforeseen data losses such as may occur due to a defective hard disk Dionex strongly recommends a regular backup on a different storage me dium For this purpose al Creating Backup Files is available in the gt Browser Backup data are compressed and stored in a different location Because GLP does not allow modification of backup data this data cannot be accessed di rectly It must be unpacked using the H Restoring Backup Files function The backup logs each single file that is copied and issues warnings if errors occur In the backup process the complete directory structure is maintained Raw Data All analog or digital values measured by a detector and stored digitally on the PC are referred to as raw data Raw data exists only for those signals that were selected by the user before the an
120. n into the CD drive 2 Start the gt Server Configuration program by selecting Server Configura tion from the Start Programs menu on the taskbar i e select Start Pro grams PeakNet 6 Server Configuration 3 Select Dionex UDP Interface under Sharable Devices and select Proper ties from the Edit or context menu 4 Select the Update Moduleware tab The names of the currently connected modules their serial numbers and current Moduleware versions are dis played Click the Select button Select the file containing the new Moduleware version and click OK The selected file appears in the Moduleware File box 7 From the list of modules select the DX 80 lon Analyzer to be updated and click the Update button Repeat this step to update any additional modules Ke Important Make certain the Moduleware matches the module type be fore updating Downloading the wrong type of Moduleware can corrupt the module s memory 8 The new Moduleware version is downloaded to the selected DX 80 To dis play the new version number in the list click the Rescan button Adding Timebases Instruments The Dionex Data System can control two DX 80 lon Analyzers via a LAN Each instrument as well as the PC on which the Dionex Data System is installed must be equipped with a 10BASE T compatible PCI LAN card the DX 80 motherboard contains a built in LAN card connected with 10BASE T LAN ca bles and have the appropriate device driver installed
121. n the Browser Setting Up a Datasource The Browser indicates only the name of the datasource not the name of the underlying database The type and number of the datasources visible to the user determine the data that can be accessed This simplified representation offers the following advantages e Data are always accessed in the same way The user does not have to worry about the data s actual storage location in the network It is not nec essary to enter the path because the location is specified when the data source is created e Each user can take advantage of a database without having to deal with special database programs Database Formats of a Datasource The Dionex Data System supports several database formats In addition to the most frequently used Access database format mdb container the data sys tem s gt ODBC Capability supports SQL Structured Query Language or da tabase formats such as ORACLE and SQL servers When installing the Dionex Data System a default datasource is created auto matically on each client PC Its name is derived from the computer name as signed during the installation under Windows installation and the addition Lo cal This guarantees that each user has a separate datasource in which per sonal results and data are stored For single systems this is the only possibil ity for data storage Raw data of each manually performed analysis is stored here Therefore do not delete the dat
122. ne Help shows the contents list Click a book icon to see further topics of a chapter Click a document icon to open a specific Help topic Continue The Continue command cancels the gt Hold and gt Stop Flow commands If a sample batch was interrupted it will resume If pump flow was interrupted it will resume Control Panel See gt Panel Data Collection Rate The data collection rate or sampling rate is the rate at which the data system collects digital data points from the detector expressed as points per second or hertz Hz A 10 Glossary Datasource and Database The term datasource is used for a database that is registered on a Dionex Data System server Setting up registering a datasource is performed in the gt Browser The user specifies the format and the location of the database For details see How to Sl Actions in the Browser Setting up a Data source During the initial Dionex Data System installation a local default datasource is created on each Client PC In network operation the datasource name is com posed of the computer name and the addendum LOCAL lt PC NAME_LOCAL gt In a local station not included on a network the name stated in the operating system below Control Panel Network Identification is used If no identification is entered the datasource is named DE FAULT_LOCAL Normally Dionex Data System data is stored in an Access database i e in an MDB Microsoft Data Base c
123. network a distinction is made between local client server installation and network installation For more details see the topics below n Local Client Server Installation n Network Installation Hardware and Software Chromatography Components 10 Local Client Server Installation The gt Client and the Chromatography gt Server are located on the same PC They must be started separately The controlled instruments in the chromatography system are connected to the PC via the LAN local area network Each chromatography server can operate a maximum of two gt Timebases chromatography systems Data are saved on the local PC During the initial Dionex Data System installa tion a local datasource is installed on each local computer If the PC is part of a local network LAN Local Area Network or WAN Wide Area Network data can also be saved externally In the same way external data can be used for data editing If the local PC is connected to a network all network installation options are available Network Installation The Client the Chromatography gt Server and the Datasource can be in stalled on different computers They are connected via the network and the cor responding network server generally a Windows NT 2000 server Each chro matography server can operate up to two systems gt Jimebases one anion system and one cation system The client server and datasource are independent units within
124. ng functions Pump Turns the pump on and off Load Inject Switches the injection valve between the load and inject positions Offset PeakNet IA subtracts the value in the Offset field from the total sig nal value sent by the DX 80 To display the total conductivity enter zero General Commands The following general commands are available Branch Starts another program and terminates the currently active program gt Log Logs device variable values in the Audit Trail Al Protocol Data Message Displays a message to the user suspends the program until the mes sage Is clicked gt Protocol Logs any message in the gt Audit Trail gt Wait Interrupts the program until a certain condition is fulfilled Delay Delays execution of the next program command for the specified time period gt Trigger Stops data acquisition and sample processing EndTrigger Continues all processes in hold mode End Starts and stops data acquisition Control 38 PGM File The program file or PGM File is a list of time precise control commands The aim is the automatic and repeated execution of specific routine tasks for ex ample automated sample processing or conditioning of a column by rinsing with solvent Monitoring of certain parameters or limits can also be performed via a PGM File The user cannot edit the PGM File Data 39 Data Data Overview Input Data Various types of input data are required for sample
125. nnecting a Database Basic Operation 34 Automatically Loading the Most Recent User Profile The Dionex Data System automatically loads the most recently used work space If this is not possible the Browser is started Opening Existing File Types or Windows Select the Open command on the File menu select the datasource and the di rectory containing the file to open and select the file type to represent Alternatively open a file by selecting it in the gt Browser File menu and dou ble clicking it Open Most Recent Files and Templates On the File and Workspace menus the lower section lists the most recently used templates and files Click a template or file to open it This is the fastest easiest way to continue an interrupted task New Select the New command from the File menu to display a list of possible file types and chromatographic operations e Select Sequence using Wizard to start the al Sequence Wizard for cre ating a gt Sequence The samples to be analyzed are entered in the se quence e Select Query using Wizard to start the Query Wizard and perform a gt Query Wizard Basic Operation 35 Printing The Dionex Data System features numerous options for presenting results You can print the screen contents or print using defined templates Printing the Screen Contents To print the contents of the currently active window select the corresponding Print command Via Print Sequence you can pr
126. nsistor Transistor Logic e E Commande Upper Lummen Validity True e eee eee A 39 d Kn EG Type Sample KEE A 29 Sample Varable A 29 Une EE Upper Limit Pressure 00 User Interface E User Mantal senian Using Manual and Online Help User Profiles Workspace v Validate ersrsrssresri ssn WV AOA ON ses Version EE Index W ee WIN H Wellness epi grestgroeregegeg COMO Rn EEGEN Device Calibrations 00000000000n Enabling and Disabling Overview Window CTGALG eege OPEN scnasena Window Types Browser Control Panel Different Method Window Table Editt cccssescssceiesesavesenetaisbenees 31 Windows 2000 17J A 17 Windows 08 Geet Windows Network Windows NT 15 17 rift Windows NT 2000 saaananaananaana0n00ananaa 8 Wizard oo 90 94 JA 40 Working Bules eee eee Workspace sisien B2A 41 Y Year 2000 A 41 Z ZIP E 85 KE A 20 A 42 Index
127. nt can be modified at any time e Open the DX 80 lon Analyzer control panel DIONEX_DX80 PAN and se lect the Connect to Timebase command from the Control menu On the left the input dialog box displays the name of the current timebase the computer name of the corresponding gt Server and the communications proto col On the right is the icon for the local computer My Computer If the com puter is connected to a network an icon for the network neighborhood is dis played also e Open the directory tree by clicking the character e lf the server is started all timebases of the local server are listed below My Computer If the server is not active or if no server is installed the mes sage The server is not running is displayed e Below Network Neighborhood is a list of all PCs currently connected to the network Open the directory tree below a computer name to see whether a Dionex Data System server is installed on this computer If the server is active the names of the corresponding timebases are visible otherwise the message The server is not running is displayed e Select the name of a timebase and complete the input by clicking OK The Dionex Data System now connects the DX 80 control panel with the corre sponding timebase If the action is successfully completed the connected in strument s can be controlled via the controls in various colors Problems with Establishing a Connection Whether a non local server i
128. ogram in an on line signal window of a control panel The Add Overlay command on the File menu can be used to overlay the current chromatogram with an earlier one The comparison chromatogram can be taken from an already processed sam ple of the current sequence or from any other sequence Also refer to MI Chromatogram Comparison A 8 Glossary Chromatographic Methods All instructions and parameters regarding sample analysis and processing are referred to as chromatographic methods Depending on the included informa tion the following distinction is made Chromatographic method Description Program files PGM Files Commands and instructions enabling automation of the analysis Quantification methods QNT All parameters required for qualitative peak identification Files and for quantitative area determination The data are stored in the gt Sequence Directory or are copied there by the data system Chromatography Server See gt Server Client Workstations that can start the Dionex Data System are called clients The cli ent provides a user interface that allows access to chromatography data and databases as well as appropriate representation of their contents To control or monitor a chromatography system from a client PC the client must be connected to the chromatography gt Server Normally this connection is established when opening a gt Control Panel As soon as there is a connec tion the user has access
129. on 55 Validation Validation Analytical data from various workstations or laboratories can be compared only if it is possible to determine the quality of the results that were produced Validation Definition The process of ensuring that a system and its analysis procedures supply re producible and reliable results is referred to as validation This includes proce dures guaranteeing the optimum technical condition of instruments hardware and firmware and of computers hardware and software especially regarding planning implementing and documenting a method Validation is thus an inte gral part of gt Good Laboratory Practice GLP When is validation necessary Instruments should be validated prior to bringing them into service and there after at regular intervals This especially applies after exchanging worn parts performing repairs or replacing an instrument The system must be validated after installing a software update also Quantification methods should be validated before they are used in daily labo ratory procedure As the quantification method changes when modifying single parameters validation is also necessary in the daily routine How is validation performed At startup many instruments perform an automatic self test to ensure optimum operation It is important to check precision detection limits the dynamic work range and the robustness of a procedure and the involved components Im portant issues
130. on of processing time under Windows 98 and Windows NT 2000 is A 18 Glossary monitored and controlled externally pre emptive This guarantees that each application process is automatically continued after a specific time A single process cannot claim all processing time at the expense of other processes Caution Pressing the key combination Alt Ctrl Del interrupts multi tasking in Windows 98 and stops all running processes It also interrupts the Dionex Data System device control Do not press the key combination Alt Ctrl Del in Win dows 98 during a running batch or during data acquisi tion This will cause data overrun and communication problems with the controlled instruments This does not apply to the Windows NT operating system Signal Noise The Signal Noise parameter indicates the signal noise within a chromatogram as calculated by the Dionex Data System The value serves as the basis for the gt Signal to Noise Ratio determined from the raw data at the start of data acqui sition Determining the Signal Noise according to the default All data points recorded during the first 10 seconds of a chromatogram form the basis for determining the noise value The Dionex Data System calculates a regression line using the method of least squares and then determines the maximum distance of a data point above and below the line When calculating the regression line all data points are weighted with their corresponding step
131. onnect a pressure gauge between the pump outlet and the pressure transducer Turn on the pump and let the system stabilize Note the average pressure reading on the gauge and then enter this reading on the line la beled Measured 7 Click the calibrate slope command button under pressure transducer The pump calibrates the slope and uploads the new value to the Dionex Data System The Dionex Data System stores this new slope as the current value 8 Turn off the pump 9 Disconnect the pressure gauge Reconnect the pressure transducer to the pump How to 103 Calibrating the Conductivity Cell When to Calibrate After installing a new cell Every 6 months Items Needed 1 0 mM KCI solution Prepare by dissolving 0 07456 g of reagent oy e ZS 8 9 grade KCI in one liter of 18 megohm deionized water Backpressure tubing to provide at least 7 MPa 1000 psi Use 0 076 mm 0 003 in ID yellow PEEK tubing P N 049715 Open the System Wellness control panel for the DX 80 lon Analyzer see How to l Opening a Wellness Control Panel Remove the two screws securing the cell to the DX 80 component mounting panel Let the cell hang by the tubing Allow the conductivity to stabilize for 5 10 minutes Click the calibrate offset command button under conductivity cell Reconnect the cell to the DX 80 component mounting panel Disconnect the pump outlet line from the injection valve connect the line di rectly to the c
132. onnected before the backup to ensure data integrity When the database is available again the Dionex Data System automatically connects to the database and writes back any data that were acquired during the breakdown DELTA Signal Property The Dionex Data System is capable of recording and evaluating signals or out put variables supplied by a detector Instead of the actual value the changes of a variable in a specific period of time can be calculated This is possible via the DELTA signal property For more information see gt Trigger Condition Demo Mode Demo Mode lets you simulate data acquisition by loading a pre recorded demo file and then displaying the data from the demo file in the gt Panel s signal win dow The demo file is read back as though the data were being acquired in real time For information on how to simulate data acquisition see How to Al Actions in the Control Panel Using Recording Demo Data Detection Code at Peak Start End AIA Peak Type This peak result variable indicates how the peak start is classified In principle this corresponds to the peak type classification used in the Dionex Data Sys tem The classification described below complies with the gt A A convention The following AIA peak types are evaluated AIA Peak Type Description Dionex Data System Peak Type B baseline peak The peak starts on the base BM bM line VD vertical drop The peak starts with a perpen MB M
133. ontainer The default datasource is also based on an Access database The gt ODBC Capability of the data system also allows using various other formats dBASE SQL Oracle etc Independently of the chosen format data of single samples gt Sample Data are saved as well as data of sequences gt Sequence Data Databases can be saved on a local hard disk or any other mass storage de vice The Directory command allows the creation of individual subdirectories below a datasource The directories are used for saving gt Sequences and corre sponding data and programs Network Database If the datasource is located on a centralized network PC the database is ac cessible by all clients that are granted the appropriate access rights by the system administrator If the datasource is stored on a local hard disk it is up to the corresponding client to decide whether the database can be accessed via the Windows File Sharing The Dionex Data System also offers the option of locking datasources directories or sequences For further details on datasources see the chapter Data X Datasource Non Availability of the Network Database In many companies and organizations the database server is shut down during the daily backup As with a network breakdown the database will not be avail able during this time In this case it is known however when the database will Glossary A 11 not be available The Dionex Data System should be disc
134. oretical information Select gt ndex on the Help menu to open an overview of online Help topics The Using Help menu item shows how to use online Help Click this button to change the mouse cursor to a question mark and then click any screen element to display a de scription of the element Click the Help button in any dialog box to display an expla nation of the dialog box by clicking the underlined term or phrase or by clicking a shortcut symbol Topics underlined with a dotted line can be viewed simultaneously with the cur rent window Introduction 3 Overview User Manual The PeakNet IA user manual contains sections on Theory Installation Op eration How to and a Glossary Use Contents and Index to search for specific terms and to get an overview of topics described in the manual Theory Installation Operation This section describes the structure and the functions of the Dionex Data Sys tem as well as basic chromatographic facts and methods When appropriate the user is referred to related topics These references are indicated as follows gt Jump term Refers to technical terms in the glossary section This type of ref erence corresponds to the green link terms in the online Help system al Shortcut sym Refers to topics in the Theory Installation Operation or How bol to sections How to This section contains answers to frequently asked questions such as How do perform a
135. ort E Displaying a Report H Peak Gumman Badi To sce oe ectseecercsece E eevee ssaeeeseeste Actions in the Window Section Calibration Plot 20000 H Printing with a Report Template DOV ICC ge d e EE Controlling the DX 80 lon Analvzer E Monitoring the DX 80 lon Analyzer Using Keyboard Shortcuts ccccccsseecsseeeseeeeseeeeeseeesseeeeseeneeseees GOSS Ql EE vi Contents Introduction 1 Introduction Using the Online Help System and the Manual The online Help system and this user manual differ only slightly in content but are intended for different situations e Refer to the online Help system to look up terms or to find information on how to use the software in a specific situation on the current screen The easiest and quickest way to receive help is by pressing the gt F1 Key For further information see al Activating Online Help e Look up information in the user manual to become familiar with the funda mental features and operation of the data system We recommend that you read the topics of the first two sections and look up unfamiliar terms in the glossary Overview Online Help The PeakNet IA online Help features various windows and levels These corre spond in general to the sections of the user manual the table of contents main section appendix and index In addition the online Help provides many context sensitive tips that can be opened only from a program wind
136. ort sheet with new column assignments D Tip Instead of a single sequence a Summary report sheet can also be created on the basis of various samples and se quences In this case a gt Query must be started in the Browser Audit Trail The SI Audit Trail can be included in a gt Report Template via a worksheet The sheet always shows the Audit Trail for the currently selected sample al Sample Protocol e The Day Time column indicates the time of a command or message e The Ret Time column indicates the corresponding retention time e The Command Message column shows the command itself a message text or an event The contents of the worksheet can be included in other documents via Copy amp Paste It is also possible to print the entire Audit Trail via the method Printer Layout 112 How to Actions in the Window Section Calibration Plot The Calibration Plot window section includes two sub windows The Calibra tion Curve section displays the calibration curve calculated from the calibration points The Calibration Function Data section lists the most important calibra tion data of the current peak In addition the following options are available e From the menu of the right mouse button select Next Peak Prev Peak to view the calibration curve of the next previous peak e Select Decoration to change the caption color and axes El Printing with a Report Template If you want to print one or several sampl
137. ow The overview displayed via the Contents button corresponds to the table of contents in the user manual e Double click a book symbol to view further levels branches e Double click a question mark symbol to open the corresponding topic e Click the Index tab to search online Help for an index term e Click the Find tab to search online Help for any non indexed string The selected topic appears in a separate Help window This window has a white background if the topic deals with questions regarding theory installation or operation The background color is yellow if practical tips are displayed How to Both windows can be displayed simultaneously Introduction Ee Note Online Help is automatically displayed in the language of the language setting see Windows Control Panel International Settings The English version is loaded by default Activating Online Help There are several ways to access online Help F1 Key How to whats this Drea tree Help menu hi Help When viewing a topic in the online Help you can gt ump to another Help topic Press the F1 key to open a Help topic containing context sensitive Help information Select How to from the menu of the right mouse button context menu to view information about operations that are currently possible practical tips Select What s this to view a description of the corresponding control or the cur rently displayed window the
138. p or de termined by the gt License Server see Al Software Protection The Dionex Data System can be started with the full range of features only if a protection device is detected i e a Dongle on the parallel PC interface or if a license provided by the license server is detected The following options are available for the Dionex Data System Basis Control RPC Timebase Client Data The basis license without additional features includes signal acquisition with one timebase Control allows controlling chromatography systems The software module must be purchased once to control any number of timebases With the RPC option the Dionex Data System can be controlled network wide For each installed Chromatography gt Server one RPC option is re quired Additional license for extending the Dionex Data System by one time base A maximum of 6 timebases per PC can be installed Additional single user license for data reprocessing on a network PC in cluding control of any number of systems in the network Additional single user license for data reprocessing on a network PC without control Hardware and Software Chromatography Components 14 Device Drivers The Dionex Data System is capable of operating analytical instruments via a uniform device independent control language The Dionex Data System uses the driver concept which means that the appropriate gt Device Driver must be loaded for
139. protocol of Sept 26 2000 A new file will be created for each day On the right section of the Browser window the daily protocols are displayed together with their names and the time of their last change A double click opens the file and displays its contents in a separate window The display cor responds to the Audit Trail control of the control panels and can also be printed In the Browser you can copy and delete daily Audit Trails D Tip The daily protocol is not overwritten by the protocol of the fol lowing day Because the Dionex Data System generates a new file for each daily protocol old protocols that are no longer re quired should be deleted from time to time Sample Protocol The sample protocol contains all Audit Trail data for the corresponding sample The sample protocol is part of each default report e Display a report in any method window and select the Audit Trail work sheet to view the Audit Trail data of the current sample The sample protocol can be activated via the Browser Open the context menu of the sample in the Browser You can open a box via Open and view the sam ple protocol via Audit Trail Samples and Sequences 49 Samples and Sequences Sample Preparation Sample preparation is a major part of a chromatographic analysis It can in clude simple procedures such as weighing solving and diluting a sample as well as more complicated physical filtration centrifugation etc and chemic
140. query How do I open the Wellness control panel How do add a timebase Appendix Glossary The glossary contains an alphabetical list of chromatographic and program related terms Refer to the glossary to view definitions of technical terms commands and parameters Index Refer to the index to locate information about a specific term Page numbers preceded by the letter A refer to the glossary section for example Command reconnect A 93 Wat A 147 Introduction 4 Introduction Hardware and Software Chromatography Components 6 Hardware and Software Chromatography Components Chromatography Instruments PeakNet IA is especially designed to control and monitor the Dionex DX 80 lon Analyzer A system may include at most one anion DX 80 and one cation DX 80 If desired a Dionex AS40 Automated Sampler may be connected If you have any questions about product support please contact your Dionex Sales Representative Device Communication To control the chromatography system and to record chromatograms the Dionex Data System constantly communicates with peripherals The data system sends control commands to the instruments Depending on the type of the exchanged data various transmission methods are used For further information see the topic below AI LAN Device Communication LAN The LAN local area network consists of at least one DX 80 lon Analyzer and a PC the PC contains a mot
141. r several samples of one sequence in the Browser and drag the sample s into an open integration window while pressing the left mouse button The action is indicated by a sign on the mouse cursor After re leasing the mouse button the chromatogram of the sample s is displayed The same channel is used as for the reference sample Select a sample in the Browser press the right mouse button and select Compare The submenu of this command includes a total list of all recorded channel types After selecting a channel the chromatograms of all samples that have raw data for this channel are compared Select a sample in the Browser press the right mouse button and select All Channels to compare all channels of a sample in a separate window If you press the F4 key or the ShifttF4 key combination the channels of all sam ples in a sequence can be displayed successively lf several samples are selected all channels of the first selected sample are displayed If you press the F4 key or the ShifttF4 key combination you can browse through the selected samples Click the Next Previous Chromatogram icons SH while holding down the Ctrl key to insert the chromatogram of the next or previous sample Perform a gt Query over several sequences or datasources Press the right mouse button and select the Compare command The submenu of this command contains a complete list of all recorded channel types After you select a channel the chromatograms of a
142. rame was selected Index Index A Acquisition On OfT Administrator Privileges 0 ATA enee 43 146 A 3 AIA Peak Type ccceceeceesceeeseee ALTZCTRLODRL Amount Caleulaton a Analytical Instrument Association ZONE yas cosceresdesvecsszescanestecsecne Autoscale ccccsscsscssscescescess EES Baseliin EE 105 Batch Drocesang Browser et E 80 BOW SEI scccesseicpecccnscspacvesasscssnonnnaes A 7 Common Features with the Windows Explorer 0000000en Differences from the Windows Replorer Funo O EE Selecting Data Calibrating eee Dale H Me el EEN Displaying a Report Function Data Information ccccccceeeccceeesceeeees Calibration Curve ccccccceeecceeee ees Calibration Data Calibration Information Calibration Plot Calibration Report Calibration Wizard cccecccceeeeeees Cell Calibration wo eeccee eens Century Compliance eeeseeeoeennnnn Chans EE SE e ET E Chromatogram 0cccccceees Chromatogram Comparison EEN Chromatogram Overlay 000 Chromatographic Methods Chromatography ee E 6 Chromatography Server CITC O E AEE EEEE T O TN Client User Interface e EE 10 li Index CMSERVER CEO Control Pile CMSETUP Loo Control Panel Command Actions 76 Acquisitio
143. rating system will crash Locking Datasources Directories and Sequences To protect data and results the Dionex Data System allows you to lock gt Data sources directories or sequences When the Locked status is enabled it is not possible to modify the corresponding object This also applies to all objects below the locked object If for example a datasource is locked all directories and sequences contained in the datasource are also locked Locked items can be recognized in the gt Browser by their icon a red lock 86 How to Proceed as follows to lock an object e Select the object in the Browser e Select the Properties command via the context menu and select the Locked check box in the edit box Proceed as follows to unlock an object e Select the object in the Browser e Select the Properties command via the context menu and clear the Locked check box in the edit box Opening Protocol Data Audit Trails From the Browser protocol data either Al Protocol Data Audit Trails for a single day Al Daily Protocols or for a single sample Sl Sample Proto cols can be activated In the server datasource daily protocols are located in the AUDIT directory un der the corresponding gt Timebase Daily protocols can be opened by double clicking To view a sample protocol the corresponding sample must be selected Select Open via the context menu right mouse click and open the sample protocol via A
144. rogram execution until the specified remote in put signal arrives During this time program time and data acquisition are stopped and the pump is kept in the Hold mode Wizard Various input procedures such as creating a gt Sequence or defining gt Query conditions are facilitated by the use of Wizards The system prompts the user to define conditions and to enter the required information The Wizard then adds default elements to complete a basic structure Glossary A 41 Workspace The area between the menu bar or gt Toolbar and the gt Status Bar is called the Workspace The workspace displays the currently open windows of the vari ous window types The workspace allows you to open a specific set of windows This is usually the most recently used workspace but you may save or open any view with a separate name The Dionex Data System stores this information in WSP Files To save settings of an individual window without changing the workspace you can use a gt Report Definition RDF File The RDF File is used whenever a sample or a window is opened individually not in connection with a workspace Combining RDF Files and a workspace allows the user to have a personal rep resentation of the screen For a detailed description refer to the AJ User Pro files chapter Year 2000 Conformity According to the document PD2000 1 1998 published by the British Standards Institution BSI year 2000 conformity shall mean
145. rol Panel logs all commands performed during sample processing and saves information regarding the entire system This includes graphical and text information The Dionex Data System classifies commands status information and error messages as indicated below System Message Warning Error Abort Error Next command Ce Symbol appears only during the batch and only if there is a connection to a data source containing the gt Sequence to process Executed instruction The color indicates the filter level The message is displayed from this level on E Green Normal CI Yellow Advanced Red Expert This color code is not valid for manual commands Protocol comments on program steps or descriptions of chromatographic condi tions Message on the screen that must be confirmed by the user Fulfilled gt Trigger condition Triggered command Manually executed command DI LOG command performed manually by the device driver BRR Y After starting data acquisition the Audit Trail window shows the start time and any subsequently performed commands Acquisition on Inject etc Even commands and status messages that are not automatically recorded e g the current system pressure can be periodically retrieved and displayed using the Log command Basic Operation 28 Of course each event included in the Audit Trail window is stored This allows you to determine later how a sample was processed and which events
146. rompted whether to over write it The following options are available Yes Yes to all No No to all and Cancel Each restored object will receive a corresponding history entry The restoration can be interrupted at any time by clicking the Cancel button Performing a Query The term gt Query refers to the search for data on the basis of specific search criteria In the Dionex Data System you can search for samples and the corre sponding sequences with user selected parameters You can either use very detailed criteria to search for one specific sample or more general criteria to search for a specific series of samples with the same properties Follow these steps to start a query e Change to the Browser and select the Query command via the right mouse button or select New Query using the Wizard on the File menu In either case a gt Wizard opens to make it easier to enter the required conditions e On the first Wizard page define the gt Data Source in which to perform the query In addition specify whether to find certain gt Sequence properties Sequences sample properties Samples and or other conditions Re sults e Define the individual conditions on the following Wizard pages All pages that refer to the properties selected on the first page will open Clicking Finish opens another dialog box which offers another four tabs e Select General to specify the datasource where the search will be per formed e
147. rotection chip gt Dongle A dongle stores the li censed serial number for one Dionex Data System station For large installations a Dionex Data System license server manages the availability of licenses Each user of a dongle protected station must enter a 12 digit gt Key Code dur ing Dionex Data System installation The key code includes all functions and options available to the user A key code matches exactly one serial number Caution It is not possible to operate several Dionex Data System sta tions with the same key code or to expand the scope of func tions on a different station Unless the entered key code matches the dongle the Dionex Data System can be oper ated only in the one hour demo mode If the Dionex Data System functions are upgraded by acquiring the corre sponding license the user receives a new key code The extended scope of functions becomes available when the new code is entered See also How to Actions in the Server Configuration Selecting the Copy Protection Location and Entering the Key Code Installation Software 20 Server Configuration Program Function The gt Server Configuration program serves to install gt Timebases on a gt Server It is required if you use the Dionex Data System to control a chroma tography system and wish to change the current instrument configuration For users who perform data evaluation only it is not absolutely necessary to be familiar with t
148. rotocol data and raw data see X Raw Data Storage File History e Select a datasource in the Browser and activate the Enable Modification History option for the context command Properties When the File History Mode is activated the Dionex Data System prompts the user for a comment on the modification or operation prior to saving any modi fied object This allows you to document all performed modifications together with the user name and the object name In combination with the Copy Cre ate and Save as commands the comment is entered automatically Objects can be samples sequences or datasources control gt Panel gt RDF gt PGM and or gt QNT files and modified chromatograms e Select a datasource or a single directory and select the Show History context command to see all objects that were modified and for which a comment was entered Data 43 Depending on the filter you use the Dionex Data System will show a list of all objects that were modified within a certain period and or by a certain operator Select an object from the list and click the Details button to view information about an object Data Export The Dionex Data System offers various data export options that enable com munication with other programs 1 You can export report pages from the gt Browser with the Batch Report command on the File menu Clicking the Export check box opens the Batch Setup dialog box where you can export report pages i
149. rrent server the datasource receives a sepa rate subdirectory for storing the corresponding raw data Below each timebase a sequence directory with a corresponding sequence is created The sequence contains a sample for manual data acquisition For detailed information on operating the Server Configuration program refer to How to Actions in the Server Configuration Program Changing the Server Configuration Basic Operation 22 Basic Operation Operation via the Keyboard All menu items can be reached via the keyboard Open the desired pull down menu by simultaneously pressing the Alt key and the underlined letter hot key Enter another hotkey to select a menu item You can also move to the desired menu item via the arrow keys Then confirm your choice by pressing the Enter key Example To execute the Align Left function on the Edit menu subse quently press the keys E A and L while you press and hold the Alt key Alternatively enter AItt tE and select a menu item via the arrow keys W and gt In addition important commands have shortcuts Shortcuts are displayed on the right hand side of menu items and functions Entering the key combination directly executes the corresponding function Example Press the Control and C keys Ctrl C to copy a selected item For a list of available shortcuts see How to Device Control Using Keyboard Shortcuts Basic Operation 23 Operation via the Mouse
150. s SQL servers Different input is required depending on the datasource type e For Access datasources the mdb container must be specified e For SQL datasources the dialog box for the ODBC driver will be opened D Tip If you want to include databases in the gt SQL or gt ORACLE formats refer to the corresponding topic How to 83 Disconnecting a Database The Datasource can be disconnected at any time if necessary Once this is done the client is denied access to the disconnected datasource e Open the gt Browser and select the Datasources command e Select the datasource in the datasource manager and click the Disconnect button D Tip The connection to a datasource can also be terminated via the Dismount Datasource command on the context menu For details about the Mount Dismount Datasource com mands see How to Datasources on Removable Media Creating a New Database The Dionex Data System enables setting up a gt Datasource with a new data base The new database is always in the Microsoft Access database format mdb container e Open the gt Browser and select the Datasources command from the File menu e Select New to create a new database e Enter the name of the new datasource this name will be displayed in the Browser e Enter the path of an empty subdirectory in which to store the database for the new datasource Use the Browse button to search for an empty direc tory Caut
151. s visible or can be accessed depends on which network protocol is selected The system requires several seconds to determine whether the selected protocol can be used If no connection is established to a specific timebase there are several possible reasons e First check whether the corresponding server is running e f the corresponding server is running try another network protocol The Dionex Data System can communicate via various network protocols eg IPX TCP IP or NetBEUI 78 How to Caution Communication between two stations is possible only if the same network protocol is installed and selected Generally it is sufficient to install the corresponding Microsoft IPX SPX compatible protocol NetBEUI TCP IP or Novell Novell IPX ODI Protocol network protocol via Settings Control Panel Network Configuration The protocol that is actually used depends on the current network installation Please contact your network administrator If a connection is to be established to a Windows NT Windows 2000 computer this is usually possible via the Named Pipes protocol sometimes the required timebase exists Server is running but is not dis played Or this can also occur when linking two computers via an ISDN connection The cause is usually a slightly incorrect network installation In this case open the Connect to Timebase dialog box again If the IPX SPX compatible protocol is used select the Activate NetBIOS with IPX
152. sadteneusscsaic Remote Procedure Call Removable Media EE EE Peak Summary nnnnnnnnnnnnnnnnennn Report Definition 00 Report Template EEN Printing with a Template Reproducibility ecceeeeeeeeees Eeer Backup Files 2 zevepstsedheesgs eegesee Calibration Data ed eer eee ere Sample Comment Preparation Processing vii eck tee EE REGISHAUM ON cs d eesietegreg seeeeksdegeee Eed KE Sample Oriented Operation Sampling Hate e Search Ouer 89 Sensitivity DICE E Signal Parameter Separation Procedures 000000000 Se Sequence Wizard nnnnnnnnneenn Serial Number Changing the Configuration Chromatography Server E re tan ce rrnirer crrree ere Eet Acquisition On OfT eege Signal to Noise Rana Signal Noise cccccccceceeeeeeeeeeees Signal Parameters Signal Plot PSC ANG eege Signal Property cccccccccceeeeeeeees Signal Window ccceceeeeeees Simulation 0 eee ccc ceeeceeeeeeeees Single User System 0 cccccceeeeee 10 Software Protection Step Sampling Rate Stop FloW c sceneresccrcerecnitiauelieecereseta Storage Requirement SUPPIESSOT eet System CHECK EE System Control System Wellness Control Panel Device Calibrations 00 Enabling and Disabling Eege Table Editor R EE EE E Tra
153. se search parameters must be further specified Depending on the type the search criterion can be linked with a value via various operators The fol lowing operators are available Operator Searches all samples Group no That are equal to the specified character string 1 2 3 4 lt gt That are not equal to the specified character string 1 2 3 4 That are larger than the specified character string 1 2 4 That are smaller than the specified character string 1 2 4 gt That are larger than or equal to the specified charac 1 2 4 ter string lt That are smaller than or equal to the specified char 1 2 4 acter string contains That contain the specified character string 1 does not contain That do not contain the specified character string 1 starts with That start with the specified character string 1 does not start with That do not start with the specified character string 1 ends with That end with the specified character string 1 does not end with That do not end with the specified character string 1 is like That fulfill the specified wildcard condition 1 is not like That do not fulfill the specified wildcard condition 1 is between That are between two values 1 2 4 is not between That are not between two values 1 2 4 during the previous That were created during a selectable time before the 4 query is null In which the variable does not exist 1 2 3 4 is not null In which the variable exists 1 2 3 4
154. sibilities Save Installation Name saves the current server configuration as a new configuration directly on the server Save as saves the current server configuration with a freely selectable name and the extension CFG in any directory The file CMSERVER CFG is not overwritten and is still valid Configuration files saved with this method are personal server configuration files These enable the user to quickly change from one installation environment to another by simply loading the file Loading the Personal Server Configuration to a Server Import To use a personal server configuration as the valid server configuration CMSERVER CFG perform the following steps Start the Server Configuration program Activate the server for which the personal server configuration is to be valid Select the Import command from the File menu and specify the name and the directory of the personal server configuration The configuration is loaded to the server Select Save to save the personal server configuration under the name CMSERVER CFG The previous file with this name is overwritten with the contents of the personal configuration All changes are indicated in the file tree of the Server Configuration program or when opening the corre sponding tab dialog boxes 72 How to Check Configuration After installing and configuring new components the current server configura tion should be checked Select the Check Configuration
155. source a directory or a single object and then select the Show History command Find Button The Find tab in the online Help window allows you to find specific words terms or text included anywhere in the online Help Full Size The Full Size function undoes all previous Zoom operations To undo only the last operation use the Unzoom function In the signal window double click in the gt Overview Window to implement the Full Size function GLP Good Laboratory Practice Good Laboratory Practice means the adherence to regulations pertaining to the organization and conditions of planning performing and monitoring laboratory work GLP contributes to enhancing the quality of test data A special focus is the complete and clear listing of the complete data in an analysis process Also regular checks of the instruments and the software are required Help see gt Online Help Glossary A 15 Hold The Hold command stops Data Acquisition and automatic gt Batch Process ing In the Hold mode no more data is acquired the pump continues delivery and processing of the batch samples is stopped before the next sample All processes can be completely aborted via the gt Stop Flow command or can be continued with the gt Continue command Index Button The Index tab dialog box contains an alphabetical list of online Help topics Enter the initial letter of the topic and select Display to view Help information
156. ssed samples Criteria for combining several samples in one sequence could for example be the same analysis conditions the same origin or the samples of the same day For detailed information on how to create a sample list see How to Actions in the Browser Creating a Sample List Samples and Sequences 52 Sequence Wizard The Sequence gt Wizard helps you quickly create a basic sample list consisting of analysis and standard samples To open the Sequence Wizard select the File gt New command in the Browser Creating a gt Sequence is performed in these easy steps e Step 1 Selecting the ion mode injection method and sample type gt Step 1 e Step 2 Selecting a timebase gt Step 2 e Step 3 Specifying unknown samples if required gt Step 3 e Step 4 Saving the sequence gt Step 4 Each step is performed on a separate screen Click one of the two arrow but tons lt Back Next gt to browse through the screens Click the lt Finish gt button in the final step to save the sequence and close the wizard Caution Do not use special characters e g the umlaut for new di rectory names or sequences as this may cause problems on Novell networks Samples and Sequences 53 Calibration Wizard The Calibration gt Wizard helps you quickly create a new calibration gt Se quence and calibrate the retention time If the system shipped to you does not include a preconfigured computer the C
157. t any time via the Print command Peak Summary Report Commonly used gt Reports such as integration reports and calibration reports contain data for just one sample In the case of a chromatogram comparison only the data of the currently selected chromatogram is displayed It is possible to compare peak data from different samples via the Peak Sum mary partial report Sample Selection e f one sample is selected and opened from the Browser the Peak Sum mary is based on the corresponding gt Sequence e f several samples are selected in the Browser only these selected sam ples are part of the Peak Summary Integration 63 e lf a gt Query is started in the Browser the search result forms the basis for the Summary In this way it is possible to compare peaks from different se quences Peak Selection e Normally no peak is selected in the chromatogram of the sample The Peak Summary Report therefore contains no entries All fields are marked n a not available or Div O Division O Only when a peak is selected manually within a chromatogram does the Peak Summary Table contain entries As soon as this is performed this peak is searched in all previously selected samples The search result is displayed in the Summary Report e lf the currently selected peak is an identified peak i e a peak labeled with its name in the peak table all involved samples are searched for this peak name The corresponding values of a
158. ta directory to view its contents in the right hand window section Files and single samples are displayed Basic Operation 29 e Double click a file or sample in the right hand window section to open the appropriate editor for a file or the corresponding chromatogram for a sam ple For convenient identification each file type sample or sequence has a type specific icon The Dionex Data System automatically recognizes the sample type calibration sample integration sample PGM File or the stored data for mat and displays the data in the corresponding chromatographic environment e Select a subdirectory a sequence or a file to drag it with the mouse to a different directory Caution For safety reasons the corresponding data are always cop ied To move files copy them and then delete the original files Directly moving files is not allowed Differences from the Windows Explorer Structure Only chromatographic data that are part of a gt Datasource are presented in the Dionex Data System Browser A datasource always represents the top level of the Browser hierarchy The datasource can be created exclusively with the Browser and cannot be com pared to the normal subdirectory in the Windows Explorer A small icon indi cates the type of data on which it is based Below the datasource level is the familiar Explorer directory structure yellow file folder The directory structure helps to manage different gt Sequen
159. that neither performance nor functionality is affected by dates prior to during and after the year 2000 In particular this means that the following rules are adhered to 1 General Integrity No value for current date will cause any interruption in op eration 2 Data Integrity Date based functionality must behave consistently for dates prior to during and after the year 2000 3 Explicit Implicit Indication of the Century In all interfaces and data storage the century in any date must be specified either explicitly or by unambiguous algorithms or inferencing rules 4 Leap Year The year 2000 must be recognized as a leap year According to the Gregorian calendar the year 1900 was not a leap year The Dionex Data System and all Dionex instruments are in compliance with the BSI rules Contact Dionex if a Year 2000 Certificate is required for any Dionex product A 42 Glossary Zoom Press the left mouse button and draw a frame around a section of a signal win dow that you wish to zoom Select gt Unzoom to undo the last zoom operation Select Full size to return to the original display 100 representation Ke Notes In the online signal window an gt Overview Window is dis played in the upper right corner in addition to the enlarged section While drawing a zoom frame the operation can be canceled by pressing the ESC key or the right mouse button Use this function to prevent redrawing the window when the wrong f
160. the Dialog Box You can edit the properties of a gt Query that has already been performed in the dialog box named Properties of Query xyz To access this dialog box click the query and select Properties on the context menu or File menu The dialog box contains the following four tabs e General tab In addition to entering a query title it is possible to specify the datasource where the query is performed If this field remains empty de fault the datasource used is the one where the query is saved The Pre ferred RDF File setting is used for opening a sample or for starting a batch report e SQL tab This tab allows you to edit the SQL condition s the samples must fulfill You can open an Edit Condition dialog box via the Edit Insert but ton by double clicking the left mouse button or via the context menu This box allows you to add Restrict Expand Condition or change SQL condi tions Change Condition 96 How to e Via Field you can select a search criterion To restrict the criterion SQL operators are available see How to n Selecting Search Criteria The corresponding comparison value can be defined in the Value edit field When editing a search criterion all sequence and sample variables can be used e Native SQL tab This tab dialog box translates the SQL statement into the SQL syntax of the corresponding ODBC driver As the default this sheet is read only and informs you about the SQL statement sent to th
161. tic gt Batch Processing the online batch For details how to create a sample list see Al Sample List Samples and Sequences 51 Sample List Sequence Table The sample list Sequence is part of the Browser It appears on the lower right window section as soon as a sequence is selected and it is read line by line One line corresponds to one sample A sample is characterized by various column entries The entries are managed in a database and are referred to as gt Sample Data To display a short expla nation of each column press the F1 key The list of samples to be processed with the characteristic sample data must be created by the user prior to each analysis There are various options e Select a cell with the mouse and then change the cell input via the key board e Select a cell with the mouse and then press F8 to open an edit dialog box that facilitates data input e Open a similar existing sequence table and modify the input e Automatically generate a sequence table via the H Sequence Wizard After entering all relevant information select the Save as command on the File menu to save the sample list with a name The number of samples per sequence is not limited but should exceed 100 only in exceptional cases The fewer samples that are added to a sequence and thus the more sequences that are created the faster single samples can be accessed and the easier it is for the user to keep track of the proce
162. tion The opera tion can be undone by selecting the Undo command on the Edit menu Select Redo to repeat the last operation Basic Operation 24 Program Start The Dionex Data System is operated exclusively via a PC equipped with Win dows 98 or Windows NT Windows 2000 As of Windows NT 4 0 both operating systems feature the same Windows 95 user interface A correctly installed Dionex Data System is started as follows Start e Click the Start button on the taskbar to open the Start menu Move the mouse cursor to the Programs menu item and wait until the submenus are displayed e Move the mouse cursor to PeakNet A submenu with several choices is displayed Client e Select PeakNet to start the data system and the data evaluation B Note Users wishing to control a chromatography system via the Dionex Data System must start the Dionex Data System server Server Server Monitor e Select Server Monitor to start the gt Sever Monitor Program On the Win dows taskbar the Dionex Data System icon will appear next to the Windows system clock B Note The Dionex Data System creates a link in the Autostart group during installation so that the program is started as soon as the computer is started You will see the corresponding icon e Move the mouse cursor over the icon You will see a quick message PeakNet Server is not running e Press the right mouse button and choose the Start Server command or double click the icon
163. tire baseline can be moved vertically The shape of the mouse cursor H indicates whether the action is possible Inserting Peaks In each free position in the chromatogram as well as on the ascending and de scending flanks of a peak a new peak i e a baseline and two peak delimiters can be inserted Places where this is possible are indicated by a small peak A on the right side of the mouse cursor When you select the Automatic Tool the mouse cursor indicates the places where a peak can or cannot be inserted Other actions edit modes are not possible at this point Manual Peak Assignment Dialog box Properties of Peak No x If you have inserted a new peak wish to name existing or unidentified peaks or wish to rename incorrectly identified peaks you can do this in the chroma togram in a report e Click the peak and then select the Peak Properties command on the con text menu to open the Properties of Peak No x dialog box or simply double click the peak e Enter the corresponding name under Component or select one of the names listed in the combo box Pressing lt Return gt or the lowest symbol bottom inserts the peak into the peak table of the QNT method using the retention time of the current chromatogram and the settings of the previous peak How to 107 Properties of Peak No 4 Component Anthracene E y Haphtahlene 2 650 min 0 295 min 0 000 min 0 28 4 0 774 min 1 167 min y
164. to the new peak start or peak end It is not possible to run over a peak delimiter the peak delimiter would be moved in the same direction After positioning the Dionex Data System draws a new baseline The modified peak properties area width amount etc are immediately updated and displayed in the integration report Peak delimiters of negative peaks can also be moved In this case the pres entation of the mouse cursor is mirrored Modifying the Baseline Two peaks that are not completely separated in a chromatogram can be inte grated individually by dropping a perpendicular line from the minimum between the two peaks to the baseline The intersection with the baseline is referred to as the baseline node When the Automatic Tool is selected each baseline node can be moved along the perpendicular line and positioned at any point the node is then re ferred to as a freely floating baseline node Freely floating baseline nodes can be generated at the end point of a baseline if desired If the mouse cursor approaches either a baseline node or a freely floating baseline node the cursor automatically clicks into place and the shape indicates which action is currently being performed The system automatically makes a distinction between moving a peak delimiter horizontally and a baseline node vertically depending on the direction in which the mouse cursor is drawn 106 How to Between two baseline nodes the en
165. udit Trail Alternatively you can view sample protocols in the Report To open the sample double click and select the Audit Trail tab How to 87 Creating Backup Files The Dionex Data System includes a program that allows you to create backup files Before starting the gt Backup the objects to archive must be selected in the gt Browser All selection options of the Browser are supported Select the Ex port Backup command on the File menu to open the Backup dialog box Backup copies of the following objects can be created When selecting a backup copy is created of a directory All subdirectories and the included files sequences etc a sequence All samples as well as files included in the sequence QNT File and PGM File individual samples The sequence information name directory title etc the se lected samples and the gt PGM and gt QNT files used by the se lected samples other files The corresponding files When using the gt Query function in the Browser these are the selection op tions When selecting a backup copy is created of individual samples The sequence information name directory title etc the se lected samples and the PGM File and QNT File used by these samples sequences All samples contained in the query of the corresponding sequence The following linked objects are also copied when creating the backup Object Link sequence Preferred gt RDF file peak table C
166. udit Trail and can be displayed on the screen or included in the printed output For details refer to the following topics gt Trigger Name gt Trigger Delay gt Trigger Validity True gt Trigger Hysteresis gt Trigger Condition gt Trigger Reaction gt Trigger Limit iP Tip A pure basis license does not include the advanced feature Trigger also see Dionex Data Systems Ej Trigger Name The name of a gt Trigger serves to identify it Therefore the trigger name must be unique for example the trigger cannot have the same name as a device or A 38 Glossary another command This allows simultaneous activation of several trigger com mands with different names Please note that the trigger command and the trigger name are separated For example 2 500 Trigger TRIGGERNAME e To quickly locate the trigger in an Audit Trail or a Report it is recommended that you enter the trigger name in capital letters Trigger Condition A gt Trigger condition is entered as follows Condition lt Parameter gt lt Operator gt lt Value gt The expressions Parameter Operator and Value have the following signifi cance Expression Description lt Parameter gt All installed channels but also parameters such as Pressure system pres sure and System Status can be used Instead of the actual value the first derivative of the signal can be evalu ated This is achieved by adding the signal property gt DELTA
167. uired options For optimum func tionality the server must be configured This is performed with the Dionex Data System Server Configuration program from any client PC If the server and the client software of the data system are located on the same PC the installation is local if not it is a network installation see n Network E Notes In the case of manual data acquisition the gt Raw Data from the system are always stored in the server directory PCNAME_LOCAL In the case of a gt Batch each user can choose where to store the data To minimize the risk of losing data raw data should always be stored temporarily on the server In the course of archiving it can be transferred to a different computer Server Configuration The Server Configuration includes information on which device is assigned to which timebase to which server a timebase is connected and the configuration of individual devices The server configuration is defined in the Server Con figuration program The information is saved in an installation file CFG file The current configu ration is always saved in the file CMSERVER CFG on the chromatography server If a user creates several installation files with the Sl Server Configuration Program these files can be transferred to any server via the Import function and used there Executing the Save command saves this configuration as the new CSERVER CFG file on the server For more information see How
168. unless the step is equidistant Adding both values supplies the first noise value The same procedure is used for the last 10 seconds of a chromatogram and supplies a further noise value ODBC Open DataBase Connectivity ODBC is the name of a standard database interface that allows access to vari ous databases e g SQL server dBASE or ORACLE via an ODBC driver Glossary A 19 Online Help The Dionex Data System Al Online Help offers help information for any situa tion on the screen e Press the Help button on the toolbar to view help information for a spe cific item on the screen e Press the F1 key to see context sensitive help information for a specific situation A description of the selected menu bar tab dialog box or acti vated control will appear e Select Index on the Help menu to view the table of contents for online Help select Using Help to see more details about using online Help Operational Qualification OQ According to the definition formulated together with EUROACHEM Operational Qualification is the process of demonstrating that an instrument will function according to its operational specification in the selected environment P Bed son and M Sargent Accred Qual Assur 1996 1 265 274 The purpose of OQ is to prove and document that your analytical system func tions according to its operating specifications while taking into account your specific environmental conditions Normally
169. utomate the performance of the Auto scale function During data acquisition this option adjusts the scaling of the signal window to exactly the size of the current chromatogram when the signal window border is exceeded in the x or y direction Glossary A 5 Autosampler Autosamplers allow automation of routine analyses A number of samples can be prepared and then processed injected in automatic gt Batch Processing A distinction is made between the following two states Loading the Sample The fluidic input and output are directly connected The solvent flow delivered by the pump is via the sampler to the column In a second cycle the volume to be injected is drawn from a sample vial into the sample loop using a syringe Injecting the Sample To inject the sample the needle is lowered into the autosampler inject port A valve directs solvent flow from the pump to the column via the sample loop and the needle seat or inject port Sample residue in the syringe is discarded to waste Autoscale The Autoscale option adjusts the scaling of the signal window exactly to the displayed window section e g the height of a peak The difference between the maximum and minimum values of the largest peak fills 80 of the signal axis in the online mode Select the gt Auto Autoscale option to perform Autoscale automatically Autozero The Autozero command resets signals to zero The subsequently measured data is interpreted an
170. ws NT computer re quires PC administrator privileges Otherwise it is not possible to create data sources or to install various system programs The Dionex Data System cannot operate correctly without these settings Installation Software 18 Under Windows NT Windows 2000 SETUP creates the user group Chrome leon Operators The user currently logged onto the system is immediately in cluded in this group All users wishing to work with the Dionex Data System must also be members of this group Under Windows NT adding new users and assigning them to various groups is via the User Manager The User Manager is accessed by clicking Start menu Programs Administrative Tools Registered users can be added to a group Datasources When generating a new datasource or connecting to an existing datasource activate the Common Datasource check box for system wide access sharing The datasource is recognized in the entire system as a system datasource Creating system datasources also requires administrator rights on the local PC All datasources that are accessed by the Dionex Data System server to proc ess sequences must be system datasources Server Access to the Network Users logging onto a PC have a personal Windows NT user account defining the currently granted rights Under Windows NT the server is installed as a system inherent service As long as the server only works on the local data source this will have no negative effects
171. zard and to open the New Query Properties dialog box where you can further specify your query also see al Specifying the Sample Query Using the Dialog Box How to 91 Selecting Search Criteria The gt Query allows you to search for samples and sequences using a variety of properties Enter the search criteria see below on either the Query Wizard Sequences page or Samples page To further restrict the query double click the SQL dialog box and enter the appropriate criteria in the Field of the Edit Conditions sheet Search Criteria Sample Name Sample Number Sample Type Sample Status Sample Comment Sample Inject Position Sample Inject Volume Sample Replicate ID Sample Inject Time Sample Dilution Factor Sample Weight Sample Amount Sample Raw ID Sample User ID Sample Program Name Sample Method Name Sequence Name Sequence Description Sequence Preferred Channel Sequence Preferred Report Sequence Directory Sequence Timebase Sequence Creation Date Sequence Creation Operator Sequence Last Update Sequence Last Update Operator 92 How to The search criteria can be divided into four different groups 1 4 see the ta ble below for available groups e Text variables eg Sample or Sequence Name 1 e Numerical variables e g Sample Number 2 e Variables with specific values Sample Type or Sample Status 3 and e Time variables Sample Inject Time Sequence Creation Date 4 The

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