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Type 50.4 Ti Rotor - American Instrument Exchange, Inc.

1. full 3 4 i SS l4 V4 7 Ng we SEKER EEN TYPE 50 4 Ti ROTOR 20 C 4 C 10 20 30 40 50 Rotor Speed K rpm Figure 1A Precipitation Curves for the Type 50 4 Ti Rotor Outer Row Using combinations of rotor speeds and homogeneous CsCl solution densities that intersect on or below these curves ensures that CsCl will not precipitate during centrifugation Fill volumes are indicated on the curves The dashed lines represent equation 6 and are shown here to illustrate the inability of that equation to guard against CsCl precipitation Type 50 4 Ti Rotor 1 90 1 80 1 70 oe S sy amp S SZ S P ALR 1 60 N S Pg ey Ss a S hd 1 50 Pi me s 7 E SS S ORN A PHS ae 1 40 e S IR KS TAS 1 30 V 1 20 TYPE 50 4 Ti ROTOR 20 C amp 4 C S t IS 1 10 SKS SS 9 1 00
2. 2007 Beckman Coulter Inc Printed in the U S A L5 TB 097G November 2007 Type 50 4 Ti Rotor Used In Beckman Coulter Class H R and S Preparative Ultracentrifuges Type 50 4 Ti Rotor SAFETY NOTICE This safety notice summarizes information basic to the safe use of the rotor described in this manual The international symbol displayed above is a reminder to the user that all safety instructions should be read and understood before operation or maintenance of this equipment is attempted When you see the symbol on other pages throughout this publication pay special attention to the specific safety information presented Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the rotor This rotor was developed manufactured and tested for safety and reliability as part of a Beckman Coulter ultracentrifuge rotor system Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter s manufacture or in a Beckman Coulter ultracentrifuge that has been modified without Beckman Coulter s approval AN EE EE EE Handle body fluids with care because they can transmit disease No known test offers complete assurance that such fluids are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Ha
3. 0 0 eee Se ee se 306812 25 Type 50 4 Ti Rotor 26 Silicone vacuum grease 1 07 2 2 ee eee ee eee 335148 Rotor Cleaning Kit 02 0 0 eee ee ee eee 339558 Beckman Solution 555 1 qt 2 2 ce eee ec eee 339555 Rotor cleaning brush 0 0 00 SE SE EE ee ee eee 339379 Centering tool for replacing overspeed disk 331325 ULTRACENTRIFUGE ROTOR WARRANTY All Beckman Coulter ultracentrifuge Fixed Angle Vertical Tube Near Vertical Tube Swinging Bucket and Airfuge rotors are warranted against defects in materials or workmanship for the time periods indicated below subject to the Warranty Conditions stated below Preparative Ultracentrifuge Rotors 5 years No Proration Analytical Ultracentrifuge Rotors 5 years No Proration ML and TL Series Ultracentrifuge ROOTS 2a Ro in E ARE Kees 5 years No Proration Airfuge Ultracentrifuge Rotors 1 year No Proration For Zonal Continuous Flow Component Test and Rock Core ultracentrifuge rotors see separate warranty Warranty Conditions as applicable 1 This warranty is valid for the time periods indicated above from the date of shipment to the original Buyer by Beckman Coulter or an authorized Beckman Coulter representative 2 This warranty extends only to the original Buyer and may not be assigned or extended to a third person without written consent of Beckman Coulter 3 This warranty covers the Beckm
4. Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center e Regularly lubricate the metal threads in the rotor with a thin even coat of Spinkote lubricant 306812 Failure to keep these threads lubricated can result in damaged threads e Regularly apply silicone vacuum grease 335148 to the O rings Replace O rings about twice a year or whenever worn or damaged e Regularly inspect the overspeed disk If it is scratched damaged or missing replace it Rotors and Tubes contains instructions for replacing the overspeed disk Refer to the chemical resistances table in Appendix A of Rotors and Tubes for chemical compatibilities of rotor and accessory materials Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center CLEANING Rotor Handle Rotor Cleaning Kit 339558 Ss O ring large 870138 Lid O ring small ME 9 Type 50 4 Ti Rotor Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred Do not allow corrosive materials to dry on the rotor Under normal use wash the rotor frequently at least weekly to prevent buildup of residues 1 Remove the O rings before washing 2 Wash the rotor and lid in a mild detergent such as Beckman Solution 555 339555 that won
5. fully loaded Ee ER Ese eg Re he EE 8 12 min Approximate deceleration time from maximum speed fully loaded EE EE EE EE Ee ee ee eee ee 7 12 min Weight of fully loaded rotor EE SE Ee se 11 kg 24 Ib Rotor material ie sawn EE SE Re eve BEE EE titanium Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed r to the standard acceleration of gravity g according to the following formula RCF 2 8 where r is the radius in millimeters is the angular velocity in radians per second 2 7 RPM 60 and g is the standard acceleration of gravity 9807 mm s After substitution RPM Type 50 4 Ti Rotor DESCRIPTION Rotor Handle w 337904 O rin sad g ET large 870138 Lid O ring small 854519 N N Rotor Body Overspeed _ Disk 330336 This rotor has been manufactured in a registered ISO 9001 or 13485 facility for use with the appropriately classified Beckman Coulter ultracentrifuges The Type 50 4 Ti rated for 50 000 rpm is a fixed angle rotor designed to centrifuge up to 44 tubes at a 20 degree angle to the axis of rotation Used in Beckman Coulter class H R and S preparative ultracentrifuges the rotor develops centrifugal forces sufficient for the differential separation of particles and for equilibrium sedimenta tion of particles such as DNA and viruses Up to 286 mL of gradient and sample v
6. t damage the rotor The Rotor Cleaning Kit 339558 contains two special plastic coated brushes and two quarts of Solution 555 for use with rotors and accessories Dilute the detergent 10 to 1 with water gt NOTE Do not wash rotor components in a dishwasher Do not soak in detergent solution for long periods such as overnight 3 Thoroughly rinse the cleaned rotor and components with distilled water 4 Air dry the rotor and lid upside down Do not use acetone to dry the rotor 5 Apply a thin even coat of silicone vacuum grease to the lid O ring before replacing it in the groove in the outer rim of the lid Also apply silicone vacuum grease to the handle O ring before reassembly Clean metal threads every 6 months or as necessary Use a brush and concentrated Solution 555 Rinse and dry thoroughly then lubricate lightly but evenly with Spinkote to coat all threads Periodically remove the O rings and wipe clean as necessary Clean the O ring grooves with a cotton tipped swab Reapply a light film of silicone vacuum grease 21 Type 50 4 Ti Rotor DECONTAMINATION If the rotor and or accessories becomes contaminated with radioac tive material it should be decontaminated using a solution that will not damage the anodized surfaces Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum RadCon Surface Spray or IsoClean Solution for soaking and Radiacwash gt NOT
7. wide offices are listed on the back cover of this manual For your convenience a partial list is given below REPLACEMENT ROTOR PARTS Type 50 4 Ti rotor assembly 0 0 0 eee eee ee 347299 Org aree tee epee dea italia die wae AR ede 870138 Oring Stal ER EED EE hte ake Bey OE ASE Oe eyed EG ES EE ea 854519 Overspeed disk 50 000 rpm 0 2 2 EE Ee ee 330336 OTHER Tubes and accessories 0 cece ee eee eens see Table 1 OptiSeal tube rack assembly 0 00 00 00 es es ee 361638 Quick Seal Cordless Tube Topper kit 60 Hz 358312 Quick Seal Cordless Tube Topper kit 50 Hz Europe 358313 Quick Seal Cordless Tube Topper kit 50 Hz Great Britain 358314 Quick Seal Cordless Tube Topper kit 50 Hz Australia 358315 Quick Seal Cordless Tube Topper kit 50 Hz Canada 367803 Tabe Topper Tack niron Iran ORE BOR EE seek BARRE EE DIR 348122 Tool kit for 19 mm and 11 mm aluminum caps 331202 includes Torque Wrench EE Ke A ess ER Re ame WE eg 858121 Sooketadaptef csujon EE EE RR EE age EE VEE EE GE EE GESE Ee 858122 Socket for 19 mm hex nUIS EE SE eee ee eee ee 858123 Tube removal tool es EDE EER BE Re EIER RE Be lg lath ae ES 301875 Floating spacer removal tool 0 0 00 SS Se eee ee eee 338765 TUDE Cap AE DE dIE EE SERRA ESS 305075 Tube removal tool Quick Seal and OptiSeal tubes 361668 Spinkote lubricant 2 0Z
8. 1 Be sure to use only those items listed and to observe the maximum speed limits and fill volumes shown Maximum fill volume is the maximum amount that can be centrifuged in the container listed Refer to Appendix A in Rotors and Tubes for chemical compatibilities of tube and acces sory materials Temperature Limits e Plastic tubes and bottles have been centrifuge tested for use at temperatures between 2 and 25 C For centrifugation at other temperatures pretest tubes under anticipated run conditions e If plastic containers are frozen before use make sure that they are thawed to at least 2 C prior to centrifugation Type 50 4 Ti Rotor Table 1 Available Tubes for the Type 50 4 Ti Rotor Use only the items listed here and observe fill volumes and maximum run speeds Tube Required Accessory Max Speed Part Max Part RCF Dimensions Description Number Fill Volume Description Number k Factor 13 x 64mm Quick Seal 344619 6 mL Delrin spacer 344389 50 000 rpm polyallomer pkg 50 312 000 x g 33 13 x 64 mm Quick Seal 344320 6 mL Delrin spacer 344389 50 000 rom Ultra Clear pkg 50 312 000 x g 33 13 x 64mm thinwall 326820 6 5 mL clear anodized 346256 50 000 rpm polyallomer pkg 50 cap 312 000 x g 33 13 x 64mm Ultra Clear 344088 6 5 mL clear anodized 346256 50 000 rpm pkg 50 cap 312 000 x g 33 13 x 48 mm OptiSeal 361621 4 7 mL Ultem spacer 361676 50 000 rpm bell top pkg 56 pkg 2
9. 1 60 Density g mL 1 40 1 20 1 00 ZA Z So i TYPE 50 4 Ti ROTOR 20 C 4 C 65 5 3 4 filled 1 2 filled 1 4 filled 96 5 lmi n Distance from Axis of Rotation mm Tmax Type 50 4 Ti Rotor Figure 2B CsCl Gradients at Equilibrium for the Type 50 4 Ti Rotor Inner Row Centrifugation of homogeneous CsCl solutions at the maximum allowable speeds from Figure 1A results in gradients presented here 19 Type 50 4 Ti Rotor CARE AND MAINTENANCE MAINTENANCE Rotor Handle 337904 O ring large 870138 Lid O ring small Check 854519 for Corrosion Centering Tool 331325 20 gt NOTE Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use e Periodically at least monthly inspect the rotor especially inside cavities for rough spots or pitting white powder deposits frequently aluminum oxide or heavy discoloration If any of these signs are evident do not run the rotor
10. 80 8 3 4 filled 1 2 filled 1 4 filled 111 5 min Tmax Distance from Axis of Rotation mm Figure 1B CsCl Gradients at Equilibrium for the Type 50 4 Ti Rotor Outer Row Centrifugation of homogeneous CsCl solutions at the maximum allowable speeds from Figure 1A results in gradients presented here 17 Type 50 4 Ti Rotor 1 90 1 80 1 70 1 60 1 50 Homogeneous CsCl Solution g mL 1 40 1 30 1 20 18 full 3 4 a a el 1 4 2 1 2 RR ky z TYPE 50 4 Ti ROTOR 20 C 4 C 10 20 30 40 50 Rotor Speed K rpm Figure 2A Precipitation Curves for the Type 50 4 Ti Rotor Inner Row Using combinations of rotor speeds and homogeneous CsCl solution densities that intersect on or below these curves ensures that CsCl will not precipitate during centrifugation Fill volumes are indicated on the curves The dashed lines represent equation 6 and are shown here to illustrate the inability of that equation to guard against CsCl precipitation 1 90 1 80 1 70
11. If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories Never exceed the maximum rated speed of the rotor and labware in use Refer to the section on RUN SPEEDS and derate the run speed as appropriate Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use Type 50 4 Ti Rotor TYPE 50 4 Ti ROTOR Axis of Rotation SPECIFICATIONS Maximum speed soeren EER enaa ES E SR E ERRA 50 000 rpm Density rating at maximum speed 000 1 2 g mL Relative Centrifugal Field at maximum speed Inner Row Outer Row At jare se 96 4 mm 270 000 x g 111 5 mm 312 000 x g At Page esc 81 1 mm 227 000 x g 96 2 mm 269 000 x g Ab Taig vere 65 7 mm 184 000 x g 80 8 mm 226 000 x g k factor at maximum speed RE pote Gendes 33 Number of tube cavities Ma OE EE OR 22 Conditions requiring speed reductions see RUN SPEEDS Available tubes i s is ies nekek tene kinni yoke bk ea see Table 1 Nominal tube dimensions largest tube 13 x 64 mm Nominal tube capacity largest tube 00 6 5 mL Nominal rotor capacity EE EE SE eee eee 268 mL Approximate acceleration time to maximum speed
12. Previously Established Run Conditions publication DS 719 RUN SPEEDS The centrifugal force at a given radius in a rotor is a function of SPEED RPMIRCF speed Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields RCF When Sa OON RAT rotational speed is adjusted so that identical samples are subjected to the same RCF in two different rotors the samples are subjected to the same force The RCF at a number of rotor speeds is provided in Table 2 11 Type 50 4 Ti Rotor 12 Table 2 Relative Centrifugal Fields for the Type 50 4 Ti Rotor Entries in this table are calculated from the formula RCF 1 12r RPM 1000 and then rounded to three significant digits Relative Centrifugal Field x g Relative Centrifugal Field x g Inner Row Outer Row Rotor Rotor Speed At max At rav At fmin k Speed At max At ray At fmin k rpm 96 4 mm 81 1 mm 65 7 mm Factor rom 111 5mm 96 2 mm 80 8 mm Factor 50000 270000 227 000 184 000 39 50 000 312 000 269 000 226 000 33 45000 219000 184 000 149 000 48 45 000 253 000 218 000 183 000 40 40 000 173 000 145 000 118 000 61 40 000 200 000 172 000 145 000 51 35 000 132 000 111 000 90 100 79 35 000 153 000 132 000 111 000 67 30 000 97 200 81 700 66 200 108 30 000 112 000 97 000 81 000 91 25 000 67 500 56 800 46 000 155 25 000 78 000 67 300 56 600 131 20 000 43 200 36 3
13. Rotors and Tubes for the use assembly and maintenance of caps e Inspect tube caps before use as described in Rotors and Tubes Replace any damaged components e Inspect the O rings or gaskets in the caps for cracks nicks or flattened areas Be sure that they are dry and free of lubricant during assembly The amp factor of the rotor is a measure of the rotor s pelleting effi ciency Beckman Coulter has calculated the amp factors for all of its preparative rotors at maximum rated speed and using full tubes The k factor is calculated from the formula N max min 1013 x 1 we 3600 where wis the angular velocity of the rotor in radians per second 0 105 x rpm rmax is the maximum radius and rmin is the minimum radius After substitution 2 533 OD IG aa k Dia 2 rpm Type 50 4 Ti Rotor Use the k factor in the following equation to estimate the run time t in hours required to pellet particles of known sedimentation coeffi cient s in Svedberg units 3 Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows 2 50 000 adj ctual run speed 4 Run times can also be estimated from data established in prior experi ments using a different rotor if the amp factor of the previous rotor is known For any two rotors a and b ta k FEE 5 ty b For more information on k factors see Use of k Factor for Estimating Run Times from
14. at 50 000 rpm see Figures 1A and 2A The same solution in a three quarter filled tube may be centrifuged no faster than 44 000 rpm or 48 000 rpm if tubes are centrifuged in the inner row only Gradient curves not shown in Figures 1B and 2B can be interpolated A tube full of the 1 64 g mL CsCl solution may be centrifuged no faster than 40 000 rpm Type 50 4 Ti Rotor TYPICAL EXAMPLES FOR DETERMINING CsCl RUN PARAMETERS At Speed Floating Components Bands Pelleted Material At Rest in Rotor Upright Example A Knowing homogeneous CsCl solution density 1 50 g mL and approximate particle buoyant densi ties 1 48 and 1 52 g mL where will particles band 1 In Figure 1A outer row find the curve that corresponds to the required run temperature 20 C and fill volume full The maximum allowable rotor speed is determined from the point where this curve intersects the homogeneous CsCl density 45 000 rpm 2 In Figure 1B sketch in a horizontal line corresponding to each particle s buoyant density 3 Mark the point in the figure where each particle density intersects the curve corresponding to the selected run speed and temperature 4 Particles will band at these locations across the tube diameter at equilibrium during centrifugation In this example particles will band about 97 and 99 mm from the axis of rotation about 2 mm of centerband to centerband separation at the rotor s 20 degree tube angle When
15. the tube is held upright there will be about 2 13 mm of centerband to centerband separation This inter band distance dup can be calculated from the formula d 0 EEU 7 ups cos0 D where dis the interband distance when the tube is held at an angle in the rotor Example B Knowing particle buoyant densities 1 70 and 1 66 g mL how do you achieve good separation 1 In Figure 1B sketch in a horizontal line corresponding to each particle s buoyant density 2 Select the curve at the required temperature 4 C and tube volume full that gives the best particle separation 3 Note the run speed along the selected curve 30 000 rpm 4 From Figure 1A select the maximum homogeneous CsCl density in this case 1 74 g mL that corresponds to the temperature and run speed established above These parameters will provide the particle banding pattern selected in Step 2 In this example particles will band at about 93 and 96 mm from the axis of rotation about 3 mm apart When the tube is held upright there will be about 3 2 mm of center of band to center of band separation 15 Type 50 4 Ti Rotor 1 90 1 80 1 70 1 60 1 50 Homogeneous CsCl Solution g mL 1 40 1 30 1 20 16
16. 00 29 400 243 20 000 50 000 43 100 36 200 204 15 000 24 300 20 400 16 600 432 15 000 28 100 24 200 20 400 363 10 000 10 800 9 080 7 360 971 10 000 12 500 10 800 9 050 816 Calculated for all Beckman Coulter preparative rotors as a measure of the rotor s relative pelleting efficiency in water at 20 C Relative Centrifugal Fields Type 50 4 Ti Rotor Inner Row 300 000 250 000 200 000 100 000 50 000 0 10 000 20 000 Speed rpm 30 000 40 000 50 000 mmm in 150000 LL 2 100 000 50 000 0 0 10 000 20 000 30 000 40 000 50 000 Speed rpm Relative Centrifugal Fields Type 50 4 Ti Rotor Outer Row 350 000 300 000 250 000 Ss Tmax x 200000 5 lay 150000 Type 50 4 Ti Rotor Do not select rotational speeds that exceed the limits in Table 1 In addition speeds must be reduced under the following circumstances 1 If nonprecipitating solutions more dense than 1 2 g mL are centri fuged the maximum allowable run speed must be reduced according to the following equation reduced maximum speed 50 000 rpm j1 2 g mL 6 p where p is the density of tube contents This speed reduction will protect the rotor from excessive stresses due to the added tube load Note however that the use of this formula may still produce maximum speeds that are higher than the limitations impo
17. 260 Nyon Switzerland Telephone 41 22 365 3707 Fax 41 22 365 0700 FRANCE Beckman Coulter France S A Paris Nord II 33 66 rue des Vanesses B P 50359 Villepinte France 95942 ROISSY CDG Cedex Telephone 33 1 49 90 90 00 Fax 33 1 49 90 90 10 e mail bioresearch france beckman com GERMANY Beckman Coulter GmbH Europark Fichtenhain B 13 47807 Krefeld Germany Telephone 49 21 51 33 35 Fax 49 21 51 33 33 e mail bioresearch de beckman com FR COULTER Beckman Coulter Inc e Sales and Service 1 800 742 2345 HONG KONG Beckman Coulter Hong Kong Ltd 12th Floor Oxford House 979 King s Road Taikoo Place Hong Kong Telephone 852 2814 7431 Fax 852 2873 4511 INDIA Beckman Coulter India Pvt Ltd Solitaire Corporate Park 3rd Floor Bldg 11 Andheri Ghatkopar Link Road Chakala Andheri West Mumbai India 400 098 Telephone 91 22 3080 5101 ITALY Beckman Coulter S p a Centro Direzionale Lombardo Palazzo F 1 Via Roma 108 20060 Cassina de Pecchi Milano Italy Telephone 39 02 953921 Fax 39 02 95392264 JAPAN Beckman Coulter K K TOC Ariake West Tower 2 5 7 Ariake Koto ku Tokyo 135 0063 Japan Telephone 81 3 5530 8500 Fax 81 3 5404 8436 MEXICO Beckman Coulter de Mexico S A de C V Avenida Popocat petl 396 Colonia Gral Pedro Maria Anaya Codigo Postal 03340 Mexico D F Mexico Telephone 001 52 55 9183 2800 NETHERLANDS Beckman Coulte
18. 312 000 x g 33 13 x 64 mm thickwall 355645 4 mL none 50 000 rpm polycarbonate pkg 25 312 000 x g 33 13 x 64 mm thickwall 355644 4 mL none 30 000 rom polyallomer pkg 25 97 200 x g 108 13 x 25 mm Quick Seal 345829 2 mL Noryl floating 345827 50 000 rpm polyallomer pkg 50 spacer 312 000 x g bell top 33 8x 51 mm thickwall 355657 1 mL Delrin adapter 303823 45 000 rom polycarbonate pkg 50 253 000 x g 40 4 Outer row bDelrin is a registered trademark of E I Du Pont de Nemours amp Company Includes disposable plastic plugs 4 Uttem and Noryl are registered trademarks of GE Plastics a _ Spacer Plug Stem oe Meniscus Base of Stem t__ _ lube 4 Type 50 4 Ti Rotor OptiSeal Tubes OptiSeal tubes come with plastic plugs and can be quickly and easily prepared for use With the tube spacer in place the g force during centrifugation ensures a tight reliable seal that protects your samples e Place the tubes in the rack and fill each tube to the base of the stem leaving no fluid in the stem Overfilling the tube can cause spillage when the plug is inserted or can compromise seal integrity However too much air can cause excessive tube defor mation disrupting gradients and sample bands e Refer to Using OptiSeal Tubes publication IN 189 included in each box of tubes for detailed information on the use and care of OptiSeal tubes Quick Seal Tube
19. E IsoClean can cause fading of colored anodized surfaces Use it only when necessary and remove it promptly from surfaces While Beckman Coulter has tested these methods and found that they do not damage components no guarantee of decontamination is expressed or implied Consult your laboratory safety officer regarding the proper decontamination methods to use If the rotor or other components are contaminated with toxic or patho genic materials follow appropriate decontamination procedures as outlined by your laboratory safety officer STERILIZATION AND DISINFECTION e The rotor and all rotor components can be autoclaved at 121 C for up to an hour Remove the lid from the rotor and place the rotor lid and spacers in the autoclave upside down Ethanol 70 or hydrogen peroxide 6 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discoloration of anod ized surfaces Use the minimum immersion time for each solution per laboratory standards 1 In U S contact Nuclear Associates New York in Eastern Europe and Commonwealth States contact Victoreen GmbH Munich in South Pacific contact Gammasonics Pty Ltd Australia in Japan contact Toyo Medic Co Ltd Tokyo 2 In U S contact Biodex Medical Systems Shirley New York internationally contact the U S office to find the dealer closest to you 3 Flammability hazard Do not use in or
20. an Coulter Centrifuge Systems only including but not limited to the centrifuge rotor and accessories and Beckman Coulter shall not be liable for damage to or loss of the user s sample non Beckman Coulter tubes adapters or other rotor contents 4 This warranty is void if the Beckman Coulter Centrifuge Sys tem is determined by Beckman Coulter to have been operated or maintained in a manner contrary to the instructions in the operator s manual s for the Beckman Coulter Centrifuge System components in use This includes but is not limited to operator misuse abuse or negligence regarding indicated maintenance procedures centrifuge and rotor classification requirements proper speed reduction for the high density of certain fluids tubes and tube caps speed reduction for precipi tating gradient materials and speed reduction for high tempera ture operation 5 Rotor bucket sets purchased concurrently with or subsequent to the purchase of a Swinging Bucket Rotor are warranted only for a term co extensive with that of the rotor for which the bucket sets are purchased 6 This warranty does not cover the failure of a Beckman Coulter rotor in a centrifuge not of Beckman Coulter manufacture or if the rotor is used in a Beckman Coulter centrifuge that has been modified without the written permission of Beckman Coulter or is used with carriers buckets belts or other devices not of Beckman Coulter manufacture 7 Rotor parts subje
21. an Coulter will repair or replace the rotor free of charge 4 If a Beckman Coulter rotor covered by this warranty is damaged due to a failure of a Beckman Coulter tube bottle tube cap spacer or adapter covered under the Conditions of this Warranty Beckman Coulter will repair or replace the rotor and repair the instrument as per the conditions in policy point 2 above and the replacement policy 5 Damage to a Beckman Coulter rotor or instrument due to the failure or malfunction of a non Beckman Coulter tube bottle tube cap spacer or adapter is not covered under this warranty although Beckman Coulter will assist in seeking compensation under the manufacturer s warranty Disclaimer IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANTABILITY AND BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER ARISING OUT OF THE MANUFAC TURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT Factory Rotor Inspection Service Beckman Coulter Inc will provide free mechanical and metallurgical inspection in Palo Alto California USA of any Beckman Coulter rotor at the request of the user Shipping charges to Beckman Coulter are the responsibility of the user Rotors will be inspected in the user s laboratory if the centrifuge in which they are used is covered by an appropriate Beckman C
22. ct to wear including but not limited to rotor O rings VTi NVT TLV MLN and TLN rotor tube cavity plugs and gaskets tubing tools optical overspeed disks bear ings seals and lubrication are excluded from this warranty and should be frequently inspected and replaced if they become worn or damaged 8 Keeping a rotor log is not mandatory but may be desirable for maintenance of good laboratory practices Repair and Replacement Policies 1 Ifa Beckman Coulter rotor is determined by Beckman Coulter to be defective Beckman Coulter will repair or replace it subject to the Warranty Conditions A replacement rotor will be warranted for the time remaining on the original rotor s warranty 2 Ifa Beckman Coulter centrifuge is damaged due to a failure of a rotor covered by this warranty Beckman Coulter will supply free of charge i all centrifuge parts required for repair except the drive unit which will be replaced at the then current price less a credit determined by the total number of revolutions or years completed provided that such a unit was manufactured or rebuilt by Beckman Coulter and ii if the centrifuge is cur rently covered by a Beckman Coulter warranty or Full Service Agreement all labor necessary for repair of the centrifuge 3 Ifa Beckman Coulter rotor covered by this warranty is dam aged due to a malfunction of a Beckman Coulter ultracentrifuge covered by an Ultracentrifuge System Service Agreement Beckm
23. f other manufacturers components or accesso ries in the Type 50 4 Ti rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Read the Safety Notice page at the front of this manual before using the rotor 1 Make sure that the rotor and lid are clean and show no signs of corrosion or cracking 2 Make sure that the 36 sector 50 000 rpm overspeed disk is properly attached to the rotor bottom If it is missing or damaged replace it according to the instructions in Rotors and Tubes 3 Check the chemical compatibilities of all materials used refer to Appendix A in Rotors and Tubes 4 Verify that the tubes being used are listed in Table 1 For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration 1 Be sure that metal threads in the rotor are clean and lightly but evenly lubricated with Spinkote lubricant 306812 Also ensure that O rings are lightly but evenly coated with silicone vacuum grease 335148 Type 50 4 Ti Rotor OPERATION 2 Load the filled containers symmetrically into the rotor Refer to TUBES AND ACCESSORIES on page 7 for information about containers If fewer than 44 tubes are being run they must be arranged symmetrically in the rotor Opposing tubes must be filled to the same level with liquid of the same density 3 Com
24. nd phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that all parts are free from pathogens and or radioactivity Steriliza tion and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be enclosed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the out side of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label printed on the RGA form when mailing the rotor and or accessories to Beckman Coulter Inc 1050 Page Mill Road Palo Alto CA 94304 Attention Returned Goods Customers located outside the United States should contact their local Beckman Coulter office Type 50 4 Ti Rotor SUPPLY LIST gt NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1 800 742 2345 in the United States or by contacting your local Beckman Coulter office See the Beckman Coulter Ultracentrifuge Rotors Tubes amp Accesso ries Catalog BR 8101 available at www beckmancoulter com for detailed information on ordering parts and supplies or contact Beckman Coulter sales 1 800 742 2345 in the United States world
25. ndle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol contain ment Do not run toxic pathogenic or radioactive materials in this centrifuge without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials in nor handle or store them near the ultracentrifuge Although rotor components and accessories made by other manufacturers may fit in the Type 50 4 Ti rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the Type 50 4 Ti rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the compo nents and accessories listed in this publication should be used in this rotor Make sure that filled containers are loaded symmetrically into the rotor and that opposing tubes are filled to the same level with liquid of the same density Make sure that cavities in use have the proper spacers inserted if applicable before installing the rotor lid
26. near operating centrifuges 22 STORAGE Type 50 4 Ti Rotor While Beckman Coulter has tested these methods and found that they do not damage the rotor or components no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfec tion is a concern consult your laboratory safety officer regarding proper methods to use Refer to publication IN 192 included in each box of tubes or bottles for tube and bottle sterilization and disinfection procedures Quick Seal and thinwall open top tubes are disposable and should be discarded after a single use When the rotor is not in use store it in a dry environment not in the instrument with the lid removed to allow air circulation so moisture will not collect in the tube cavities 23 Type 50 4 Ti Rotor 24 RETURNING A ROTOR Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc This RGA form may be obtained from your local Beckman Coulter sales office and should contain the following information e serial number e history of use approximate frequency of use e reason for the return e original purchase order number billing number and shipping number if possible e name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory and e name a
27. olume can be centrifuged per run The rotor is made of titanium and is finished with black polyurethane paint The aluminum lid and handle are anodized for corrosion resis tance O rings made of Buna N rubber in the lid and handle maintain atmospheric pressure inside the rotor during centrifugation if they are properly lubricated Four small holes in the lid provide a temporary vent preventing pressure build up and extrusion of the large lid O ring in the event of tube leakage Because of the weight of the rotor drive pins are not required in the rotor drive hub cavity For overspeed protection a photoelectrical detector in the ultracentri fuge monitors the overspeed disk on the rotor bottom and shuts down the run if speeds exceeding 50 000 rpm are detected See the Warranty at the back of this manual for warranty information PREPARATION AND USE Specific information about the Type 50 4 Ti rotor is given here Infor mation common to this and other rotors is contained in Rotors and Tubes for Preparative Ultracentrifuges publication LR IM which should be used together with this manual for complete rotor and accessory operation PRERUN SAFETY CHECKS 4 Tro S G mm 26 0000 WA 330336 o Cay ROTOR PREPARATION Type 50 4 Ti Rotor ID NOTE Although rotor components and accessories made by other manufacturers may fit in the Type 50 4 Ti rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use o
28. oulter Service Agreement Contact your local Beckman Coulter office for details of service coverage or cost Before shipping contact the nearest Beckman Coulter Sales and Service office and request a Returned Goods Authorization RGA form and packaging instructions Please include the complete rotor assembly with buckets lid handle tube cavity caps etc A SIGNED STATEMENT THAT THE ROTOR AND ACCESSO RIES ARE NON RADIOACTIVE NON PATHOGENIC NON TOXIC AND OTHERWISE SAFE TO SHIP AND HANDLE IS REQUIRED Beckman Coulter Worldwide Biomedical Research Division Offices AUSTRALIA Beckman Coulter Australia Pty Ltd Unit D 24 College St Gladesville NSW 2111 Australia Telephone 61 2 9844 6000 or toll free 1 800 060 880 Fax 61 2 9844 6096 email lifescienceaustralia beckman com CANADA Beckman Coulter Canada Inc 6755 Mississauga Road Suite 600 Mississauga Ontario Canada L5N 7Y2 Telephone 1 905 819 1234 Fax 1 905 819 1485 CHINA Beckman Coulter Inc Beijing Representative Office Unit 2005A 2006 2009 East Ocean Center Jian Guomenwai Avenue Beijing 100004 China Telephone 86 10 6515 6028 Fax 86 10 6515 6025 6515 6026 CZECH REPUBLIC Beckman Coulter Prague Radiova 1 102 27 Prague 10 Czech Republice Telephone 420 267 00 85 13 Fax 420 267 00 83 23 EASTERN EUROPE MIDDLE EAST NORTH AFRICA Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1
29. plete loading by placing the correct spacers if required over the tubes gt NOTE Place filled tubes in at least two opposing cavities Make sure that cavities in use have the proper spacers inserted if applicable before installing the rotor lid 4 Put the lid in place and tighten by hand as firmly as possible N WARNING Always run the rotor with the handle firmly securing the lid to the rotor 1 Carefully lower the rotor straight down onto the drive hub 2 Refer to the applicable instrument instruction manual for ultracen trifuge operation 3 For additional operating information see the following e RUN TIMES page 10 for using k factors to adjust run durations e RUN SPEEDS page 11 for information about speed limitations e SELECTING CsCl GRADIENTS page 13 for methods to avoid CsCl precipitation during centrifugation Type 50 4 Ti Rotor REMOVAL AND SAMPLE RECOVERY Quick Seal Tube Removal Tool 361668 TUBES AND ACCESSORIES J A CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories 1 Remove the rotor from the centrifuge by lifting it straight up and off the drive hub 2 Remove the rotor lid 3 Use the appropriate removal tool listed in the SUPPLY LIST to remove the spacers and tubes The Type 50 4 Ti rotor uses the tubes listed in Table
30. r Nederland B V Nijverheidsweg 21 3641 RP Mijdrecht Postbus 47 3640 AA Mijdrecht The Netherlands Telephone 31 297 230630 Fax 31 297 288082 SINGAPORE Beckman Coulter Singapore Pte Ltd 116 Changi Road Unit 03 01 02 Singapore 419718 Telephone 65 6339 3633 Fax 65 6336 6303 SOUTH AFRICA SUB SAHARAN AFRICA Beckman Coulter Stand 1A Primegro Park Tonetti Street 1685 Halfway House Johannesburg Republic of South Africa Telephone 27 11 805 2014 5 Fax 27 11 805 4120 e mail beckman intekom co za SPAIN Beckman Coulter Espafia S A C Caleruega 81 28033 Madrid Spain Telephone 34 91 3836080 Fax 34 91 3836096 email bioresearch espana beckman com SWEDEN Beckman Coulter AB Archimedesvaegen 7 Box 111 56 SE 168 11 Bromma Sweden Telephone 46 8 564 85 900 Telefax 46 8 564 85 901 SWITZERLAND Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 0800 850 810 Fax 41 0848 850 810 TAIWAN Beckman Coulter Taiwan Inc Taiwan Branch 8th Floor 216 Tun Hwa South Road Section 2 Taipei 106 Taiwan Republic of China Telephone 886 2 2378 3456 Fax 886 2 2377 0408 TURKEY Beckman Coulter Ltd E 5 Yanyol Faith Cad 81410 Soganlik Kartal Istanbul Turkey Telephone 90 216 309 1900 Fax 90 216 309 0090 UNITED KINGDOM Beckman Coulter United Kingdom Ltd Oakley Court Kingsmead Bu
31. s Quick Seal tubes must be sealed prior to centrifugation These tubes are heat sealed and do not need caps however spacers are required on top of the tubes when they are loaded into the rotor e Fill Quick Seal tubes leaving a small bubble of air at the base of the neck Do not leave a large air space too much air can cause excessive tube deformation Refer to Rotors and Tubes for detailed information on the use and care of Quick Seal tubes Thinwall Tubes Thinwall polyallomer and Ultra Clear tubes require caps for tube support Fill the tubes as full as possible to prevent tube collapse during centrifugation If necessary float mineral oil or some other low density immiscible liquid on top of the tube contents to fill the tube to its maximum volume Do not use an oil overlay in Ultra Clear tubes Type 50 4 Ti Rotor 10 RUN TIMES TIME HR MIN m I tL ee an Thickwall Tubes Thickwall polyallomer and polycarbonate tubes can be run partially filled at least half filled with or without caps but all opposing tubes for a run must be filled to the same level with liquid of the same density Do not overfill capless tubes be sure to note the reductions in fill volume and run speed shown in Table 1 Tube Caps Thinwall tubes require caps for tube support during centrifugation thickwall tubes can be run without caps speed reduction may be required Use only the caps listed in Table 1 Refer to
32. sed by the use of certain tubes or adapters see Table 1 In such cases use the lower of the two speeds 2 Further speed limits must be imposed when CsCl or other self forming gradient salts are centrifuged as equation 6 does not predict concentration limits speeds that are required to avoid precipitation of salt crystals Solid CsCl has a density of 4 g mL and if precipitated during centrifugation may cause catastrophic rotor failure and instrument damage Figures 1 and 2 together with the description and examples below show how to reduce run speeds when using CsCl gradients SELECTING CsCl GRADIENTS Precipitation during centrifugation would alter density distribution and this would change the position of the sample bands Curves in Figures 1 and 2 are provided up to the maximum rated speed of the rotor but note also that tubes or bottles must never be centrifuged faster than the limits in Table 1 gt NOTE The curves in Figures 1 and 2 are for solutions of CsCl salt dissolved in distilled water only If other salts are present in significant concentra tions the overall CsCl concentration may need to be reduced 13 Type 50 4 Ti Rotor 14 ADJUSTING FILL VOLUMES Buffer Gradient Rotor speed is used to control the slope of a CsCl density gradient and must be limited so that CsCl precipitation is avoided Speed and density combinations that intersect on or below the curves in Figures 1A and 2A ensure tha
33. siness Park London Road High Wycombe Buckinghamshire HP11 1JU England U K Telephone 44 01494 441181 Fax 44 01494 447558 e mail beckman_uk beckman com Additional addresses are available at www beckmancoulter com 4300 N Harbor Boulevard Box 3100 Fullerton California 92834 3100 Internet www beckmancoulter com e Telex 678413 Fax 1 800 643 4366 2007 Beckman Coulter Inc de Bd Printed on recycled paper
34. t CsCl will not precipitate during centrifugation in the Type 50 4 Ti rotor Curves are provided at two temperatures 20 C black curves and 4 C gray curves The reference curves in Figures 1B and 2B show gradient distribution at equilibrium Each curve is within the density limits allowed for the Type 50 4 Ti rotor each curve was generated for a single run speed using the maximum allowable homogeneous CsCl densities one for each fill level that avoid precipitation at that speed The gradients in Figures 1B and 2B can be generated from step or linear gradients or from homogeneous solutions But the total amount of CsCl in solu tion must be equivalent to a homogeneous solution corresponding to the concentrations specified in Figures 1A and 2A Figures 1B and 2B can also be used to approximate the banding positions of sample particles Figures 1 and 2 show that several fill volumes are possible in a tube If a tube is partially filled with gradient solution float mineral oil or some other low density immiscible liquid on top of the tube contents to fill the tube to its maximum volume Do not use an oil overlay in Ultra Clear tubes Note that for a given CsCl density as the fill level decreases the maximum allowable speed increases Partial filling may be desirable when there is little sample or when you wish to shorten the run time For example a half filled tube of 1 64 g mL homogeneous CsCl solution at 20 C may be centrifuged

Type 50.4 Ti Rotor - American Instrument Exchange, Inc.

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