Mitos P-Pump Range - Dolomite Microfluidics
Contents
1. download Mitos Sensor Units connection MAR 000182_v 1 8 Page 12 of 58 The following accessories are supplied with the Mitos P Pump e Pneumatic supply cable e Tubing FEP 250um ID 1 16 OD 2m length e Power supply unit e Mains IEC lead User to supply the following e Vessel containing sample liquid o Liquids to be pumped must always be placed in a suitable vial within the glass chamber Never place any liquid directly in the glass chamber e Compressed air gas supply 4 2 Fluidic Connection 4 2 1 Opening Chamber Locked symbol aligned Two stage opening process Unlocked symbol aligned push down amp rotate anticlockwise O O pull upwards slight turn anticlockwise amp pull upwards to open MAR 000182_v 1 8 Page 13 of 58 The Dolomite Centre Ltd 4 2 2 Inserting Fluid Vial The fluid to be pumped must always be placed inside a small vial and not directly into the chamber Insert fluid vial Connect dip tube Guide dip tube into vial place fluid vial smaller than insert 1 16 OD tube into as the chamber lid is put in 330 x H88mm into chamber chamber lid position ensure that the dip tube is guided into the fluid use vessel holder for small leave length of tube vial fluid vials protruding and tighten fitting 4 2 3 Closing Chamber Unlocked symbol aligned Two stage closing process Locked symbol aligned push down slight turn clockwise and push down O rotate clockwise until lid click2. MITOS P PUMP RANGE ial USER MANUAL Mitos P Pump range Product picture Product description The Mitos P Pump provides pulseless liquid flow with a precise pressure driven pumpin Mitos P Pump p P Poe mechanism It operates over a wide pressure Part No 3200016 range 0 10bar with excellent response time and accuracy The Mitos P Pump Basic provides pulseless liquid flow with a precise pressure driven Mitos P Pump Basic pumping mechanism Connecting to a PC via Part No 3200175 RS232 serial interface allowing data logging and control using free PC software The Mitos P Pump Remote provides pulseless liquid flow operating over a wide pressure range Mitos P Pump Remote e 0 10bar with excellent response time and Part No 3200176 accuracy The design allows users to connect to Dolomite Remote Chambers or custom chambers with a push click action The Mitos P Pump Remote Basic connects to a Mitos P Pump PC allowing data logging and control using free Remote Basic es PC software The design allows users to Part No 3200177 A connect to Dolomite Remote Chambers o custom chambers with a push click action All four pumps in the Mitos P Pump range provide pulseless liquid flow with a precise pressure driven pumping mechanism The pumps operate over a wide pressure range 0 10bar Please note the Basic versions of the pumps will not work without PC control MAR 000182_v 1 8 Page 2 of 58 Note
3. Prevents flow rate instability To begin test work with the Mitos P Pump range Enables fast and reliable connection of compressed air or gas supplies Providing a low cost method of delivering 3 reagents toa microfluidic system Page 47 of 58 The Dolomite Centre Ltd Mitos P Pump 3 way Vessel Holder Kit Part No 3200045 Mitos P Pump Remote Chamber 400 Part No 3200043 Mitos P Pump Remote Chamber 30 Part No 3200178 Dip Tube Fitting Part No 3200143 USB to RS232 Adapter Cable Part No 3200197 MAR 000182_v 1 8 This Vessel Holder is designed to accommodate 3 micro tubes in a Mitos P Pump The Remote Chamber enables greater input volumes up to 400 ml to be used with a Mitos P Pump The Mitos P Pump Remote Chamber 30 is a 0 10bar lockable glass pressure chamber The Dip Tube Fitting allows users to easily connect a dip tube into a fluid vessel on a Mitos P Pump For PCs that do not support an RS232 serial interface Dolomite offers a USB to RS232 Adapter Cable Improves ease of use when using the Mitos P Pump for 3 channel pumping Continuous pumping Option for heating and magnetic stirring Easy to view sample liquid levels Sample volumes from 100ul to 30ml Option for heating and magnetic stirring Easy to view sample liquid levels Featuring an in built strain relief Compatible with 1 16 OD tubing and 1 4 28 flat bottom ports Allowing users to co
4. CE Declaration of Conformity In accordance with EN 45014 1998 We The Dolomite Centre Ltd of Unit 1 Anglian Business Park Orchard Road Royston Herts SG8 5TW UK declare that Equipment Mitos P Pump Remote Model name Part number 3200176 in accordance with the following Directive s 73 23 EEC The Low Voltage Directive and its amending directives 89 336 EEC The Electromagnetic Compatibility Directive and its amending directives has been designed and manufactured to the following specifications EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements EN 61326 2 2 2006 Electrical equipment for measurement control and laboratory use EMC requirements EN 61000 4 3 2002 EMC Immunity EN 55022 2006 EMC Radiated current emissions hereby declare that the equipment named above has been designed to comply with the relevant sections of the above referenced specifications The unit complies with all Unit 1 Anglian Business Park Orchard Road Royston Herts SG8 5TW applicable essential requirements of the Directives UK on 8 September 2011 nn MAR 000182 v 1 8 Page 56 of 58 HNE DYS oo ceeds EE E E Name Andrew Lovatt Position CEO Done at The Dolomite Centre Ltd The Dolomite Centre Ltd The Dolomite Centre Limited Unit 1 Anglian Business Park Orchard Road Reo ees ar eN Royston Hertfordshire SG8 STW UK Royston
5. Cable Part No 3200197 all devices will be detected automatically by the PC software and shown in the P Pumps section see point 9 1 2 2 Serial converter and serial cables If the pump is connected via a generic serial converter or to a serial port the devices will not be detected automatically but have to be added manually following the step by step process below 1 Start the PC Software and open the Devices section 2 Click on the following icon top left corner to show all COM ports connected to the PC which might take up to 15 seconds 3 Select the COM port that connects the pump to the PC 4 lf the serial converter or serial cable you are using offers more than one COM port you might need to select all ports try the different COM ports to identify the right port that connects the pump to the PC 5 The PC Software will scan the COM port ports selected which might take a couple of seconds 6 After the scanning has been completed the COM port which connects the pump to the PC will disappear from the COM ports list and the pump will be shown in the P Pumps section MAR 000182 v 1 8 Page 41 of 58 MM 11 2 Using the PC software The PC Software offers 3 sections as shown below 1 Devices This section allows users to monitor and set the target and chamber pressure of the pump It is divided into a P Pumps section showing all pumps connected to the PC Software and Flow Sensors section show
6. Hertfordshire SG8 5HW UK Company No 05640084 T 44 0 1763 242491 F 44 0 1763 246125 W www dolomite centre com dolomite CE Declaration of Conformity In accordance with EN 45014 1998 We The Dolomite Centre Ltd of Unit 1 Anglian Business Park Orchard Road Royston Herts SG8 5TW UK declare that Equipment Mitos P Pump Remote Basic Model name Part number 3200177 in accordance with the following Directive s 73 23 EEC The Low Voltage Directive and its amending directives 89 336 EEC The Electromagnetic Compatibility Directive and its amending directives has been designed and manufactured to the following specifications EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements EN 61326 2 2 2006 Electrical equipment for measurement control and laboratory use EMC requirements EN 61000 4 3 2002 EMC Immunity EN 55022 2006 EMC Radiated current emissions hereby declare that the equipment named above has been designed to comply with the relevant sections of the above referenced specifications The unit complies with all Unit 1 Anglian Business Park Orchard Road Royston Herts SG8 5TW applicable essential requirements of the Directives UK on 8 September 2011 MAR 000182_v 1 8 Page 57 of 58 SNOT DYS arae CO ul O Name Andrew Lovatt Position CEO Done at The Dolomite Centre Ltd
7. The Mitos P Pump should not be used for prolonged periods with acetone After being used with acetone the chamber lid should be removed to allow the pressure chamber to be ventilated Acetone must never be stored in the Mitos P Pump For more information on the Mitos P Pump product range please visit our website http www dolomite microfluidics com webshop and download our technical datasheets Closed loop Flow Control for the Mitos P Pump Please see separate Mitos P Pump Flow Control user instructions for details on how to operate using closed loop flow control Closed loop flow control is an enhancement to the Mitos P Pump system for improved control of flow rates from 70 nl min to 5 ml min It requires that a Mitos Flow Rate Sensor Part No 3200096 3200100 is connected in line between the pump and the microfluidic device Once the pump detects that a flow rate sensor is connected the flow control menu options are enabled A target flow rate can then be set on the P Pump and the pump pressure is automatically adjusted to meet the target flow rate System for Flow Control Mitos P Pump Part No 3200016 with Mitos Sensor Display Part No 3200095 and Mitos Flow Rate Sensor Part No 3200096 3200100 The key benefits of closed loop flow control are MAR 000182_v 1 8 Page 3 of 58 e Control flow rates instead of pressure making experiments easier to set up and optimise e Improved flow rate precision even better co
8. _P_ Mitos P Pump connected system As P 0 10 bar flow resistance Rs f d L Key P pressure set by user bar Rs flow resistance mm d tube internal diameter mm L tube length mm Q flow rate ul min u viscosity cP Flow rate dependence on flow resistance MAR 000182_v 1 8 Page 10 of 58 The flow resistance of a length of tube or circular channel is a function of the internal diameter and length A long tube with small internal diameter gives a high flow resistance This can be calculated with the following formula and used to calculate the flow rate in the system when pumping at a selected pressure flow resistance of flow rate generated by tube or channel a selected pressure Rs 6 79x10 L Insert into formula Q P lt gt El d Rs Please note the correct units must be used as defined above Formulae for flow rate calculation For more information on using flow resistors with the Mitos P Pump range please see section 10 MAR 000182_v 1 8 Page 11 of 58 4 Mitos P Pump Part No 3200016 4 1 Features Flow sensor module dock Dip tube liquid outlet Pressure chamber lid Glass pressure chamber Display screen gt Twist and click control knob n Cancel button y A OS Pressure supply connection Power supply Mitos Sensor Units connection electrical connection l USB B connection ON OFF switch from PC Not used USB A connection x 2 for data
9. atmosphere The timer can be started after the Mitos P Pump and Mitos P Pump Remote is already controlling at a pressure to vent the chamber in a set period of time Alternatively the timer can be used to start and end pressure control This function is particularly useful in stopping the pump before the sample fluid has been used up In the main menu select option 2 to access the timer screen countdown time h m s Click scroll click to set the time inh m s play pause control and target pressure Click play to start control target pressure Click again to pause the timer Pressure control will continue Back in the main menu press back button Back in the pressure control screen timer symbol E Timer symbol is displayed 10 5 Menu Option Configuration Units The Mitos P Pump and Mitos P Pump Remote can be configured to operate in pressure units of mbar bar or psi In the main menu select option 3 to access configuration options In the configuration menu select option 1 to access unit selection Scroll and click to select the required units Scroll MAR 000182_v 1 8 Page 36 of 58 10 6 Menu Option Configuration Mode The Mitos P Pump and Mitos P Pump Remote have two main modes of use The first is static mode where the user selects a target pressure and the pump controls to that pressure This is used in the majority of situations The second is moving mode where th
10. same as for the Mitos P Pump Remote Part No 3200177 including pulseless pumping performance and easy to use pneumatic fitting 7 1 Features Flow sensor Pneumatic fitting A O On Off switch A Pressure supply connection Mitos Sensor Interface connection Power supply connection RS232 serial connection to a PC MAR 000182_v 1 8 Page 25 of 58 The following accessories are supplied with the Mitos P Pump Remote Basic e 2x Pneumatic supply cable e Tubing FEP 250um ID 1 16 OD 2m length e Power supply unit e Mains IEC lead User to supply the following e Suitable Windows PC e Vessel containing sample liquid e Compressed air gas supply 7 2 Pneumatic Connection y a E Muy ont COM ECT TO PORT To connect pneumatic supply To disconnect pneumatic supply use pneumatic supply cable included to link turn off compressed air gas supply first compressed air gas supply to the Mitos P Pump Remote Basic push in sleeve of pressure supply connector to release either port can be used MAR 000182_v 1 8 Page 26 of 58 7 3 Power Connection Insert power cable Press button to switch on e only use the power supply unit provided e Front of the pump press 1 to switch ON 7 4 USB to RS232 Connection Insert RS232 connector e connect USB to PC The Mitos P Pump Remote Basic connects to a PC via RS232 serial interface allowing data logging and control using free PC software
11. the glass vessel Maintenance Advice Maintenance should only be attempted by qualified service personnel Removal of the back panel may invalidate any warranty Please contact Dolomite for further information General Advice on Use of Pressure Equipment Care must be taken when the Mitos P Pump is pressurised to ensure that the unit is not dropped or damaged in any way Use of Oxygen and flammable gases P Pump should not be used with Oxygen or flammable gases Eye Protection Safety glasses should be worn at all times when using a Mitos P Pump This is due to the use of pressurised equipment and is especially important when hazardous liquids are used MAR 000182 v 1 8 Page 7 of 58 2 Getting Started 2 1 Introduction Xx 4 A lt atmospheric ff pressure f y ya E flow f liquid flow x r 5 chamber pressure The Mitos P Pump range delivers fluid by a pressure driven pumping mechanism The user sets the chamber pressure gauge pressure which determines the flow rate of liquid from the chamber When the user vents the chamber or when the Mitos P Pump range is switched off the pressure inside the chamber returns to atmospheric and flow stops Gauge pressure The pressure relative to atmospheric pressure ambient air Absolute pressure The pressure relative to a perfect vacuum MAR 000182_v 1 8 Page 8 of 58 3 Understanding Pressure Driven Flow The pressure driven flow concept
12. 8 5TW UK declare that Equipment Mitos P Pump Basic Model name Part number 3200175 in accordance with the following Directive s 73 23 EEC The Low Voltage Directive and its amending directives 89 336 EEC The Electromagnetic Compatibility Directive and its amending directives has been designed and manufactured to the following specifications EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements EN 61326 2 2 2006 Electrical equipment for measurement control and laboratory use EMC requirements EN 61000 4 3 2002 EMC Immunity EN 55022 2006 EMC Radiated current emissions hereby declare that the equipment named above has been designed to comply with the relevant sections of the above referenced specifications The unit complies with all Unit 1 Anglian Business Park Orchard Road Royston Herts SG8 5TW applicable essential requirements of the Directives UK on 8 September 2011 MAR 000182_v 1 8 Page 55 of 58 SNOT DYS arae CO ul O Name Andrew Lovatt Position CEO Done at The Dolomite Centre Ltd The Dolomite Centre Ltd The Dolomite Centre Limited Unit 1 Anglian Business Park Orchard Road A eee Greene sere Was i toyston ordshire SG8 SHW UK Royston Hertfordshire SG8 5TW UK ERO A ORS IAC SERNEUS Company No 05640084 T 44 0 1763 242491 F 44 0 1763 246125 W www dolomite centre com dolomite
13. Connection to the top of the Mitos P Pump 8 3 USB Connection 8 4 Electrical Connection 9 Controlling the pumps 10 Manual User Control 10 1 Pressure Control 10 2 User Controls 10 3 Menu option Tare 10 4 Menu option Timer 10 5 Menu option Configuration Units 10 6 Menu option Configuration Mode 10 7 Menu option Configuration Resolution 10 8 Menu option Configuration Calibration 10 9 Menu option Error log 10 10 Menu option USB data export 10 11 Menu option Screensaver 10 12 Menu option Information PC Software User Control 9 1 1 Detect devices 9 1 1 1 USB Connection 9 1 1 2 Connection via a serial cable 9 1 2 Using the PC Software Using Flow Resistors 10 1 Flow Resistors 10 2 Flow Rate Calibration Accessories 11 1 Accessories compatibility FAQ Power consumption specification CE Declaration of Conformity MAR 000182_v 1 8 28 29 30 30 31 32 32 33 34 35 36 36 36 37 37 38 39 39 40 40 40 40 41 43 43 45 46 48 49 53 54 Page 6 of 58 1 Important Information Overpressure Warning Do not connect a pressure supply over 11 bar gauge to the Mitos P Pump range This may result in failure of the Mitos P Pump range and injury to the user Glass Pressure Chamber Warning If the glass pressure chamber is scratched or cracked do not continue to use the Mitos P Pump This may result in failure of the Mitos P Pump and injury to the user Please contact Dolomite for replacement of
14. Features Flow sensor module dock Dip tube liquid aiitlat Pressure chamber lid Glass pressure chamber Pressure supply connection Mitos Sensor Units electrical connection Power supply connection RS232 serial connection toa PC MAR 000182_v 1 8 Page 17 of 58 The Dolomite Centre Ltd The following accessories are supplied with the Mitos P Pump Basic e Pneumatic supply cable e Tubing FEP 250um ID 1 16 OD 2m length e Power supply unit e Mains IEC lead User to supply the following e Suitable Windows PC e Vessel containing sample liquid o Liquids to be pumped must always be placed in a suitable vial within the glass chamber Never place any liquid directly in the glass chamber e Compressed air gas supply For information regarding fluidic connection to the Mitos P Pump Basic please see 4 2 5 2 Pneumatic Connection e g Muy pao A TO PORT To connect pneumatic supply e use pneumatic supply cable included to link compressed air gas supply to the Mitos P Pump Basic e either port can be used y To disconnect pneumatic supply turn off compressed air gas supply first push in sleeve of pressure supply connector to release MAR 000182_v 1 8 Page 18 of 58 5 3 Power Connection Insert power cable Press button to switch on e only use the power supply unit provided e front of the pump press the button to switch ON 5 4 USB to RS232 C
15. For PCs that do not support an RS232 serial interface Dolomite offer a USB adapter cable Part No 3200197 MAR 000182_v 1 8 Page 27 of 58 7 5 Connection to Remote Chamber The Mitos P Pump Remote Basic features a pneumatic fitting which allows the connection to Dolomite s Remote Chambers or custom chambers The Mitos P Pump Remote Basic can connect to the Mitos P Pump Remote Chamber 30 Part No 3200178 a glass pressure chamber which connects securely to the pump via a pneumatic fitting The Mitos P Pump Remote Chamber 30 can be placed on a hotplate magnetic stirrer or incubator to benefit applications requiring temperature control Connect Mitos P Pump Remote Chamber 30 Pneumatic tubing is connected to the pump and connect via provided pneumatic tubing ene ice ane 3m lengt gth between experiments For information regarding fluidic connection to the Mitos P Pump Remote Chamber 30 please see 5 2 7 6 Mitos Sensor Unit Electrical Connection To connect to Mitos Sensor Unit e The Mitos Sensor Units can also connect to the Mitos P Pump range via a fluidic connection for more information please see section 8 e use electrical cable supplied with the Mitos Sensor Unit MAR 000182_v 1 8 Page 28 of 58 8 Mitos Sensor Units Connection The Mitos Sensor Display Part No 3200095 and Mitos Flow Rate Sensors Part No 3200096 3200100 are compatible with the Mitos P Pump range for measuring and displaying ultra low
16. applies to all pumps within the Mitos P Pump range whether the pressure chamber is part of the pump module i e Mitos P Pump and Mitos P Pump Basic or is connected to the pump via a pneumatic connection i e Mitos P Pump Remote and Mitos P Pump Remote Basic 3 1 The Basics If the Mitos P Pump range is set to 5bar pressure and water is pumped out through a short section of tube the water will be pumped out at a high flow rate If the short section of tube is replaced with a very long section of tube with a very small internal diameter the water flow rate will slow down considerably Microfluidic channels are equivalent to a tube with a very small internal diameter If the water is replaced with honey then the flow rate will slow down even further if it flows at all This is due to the high viscosity of honey MAR 000182_v 1 8 Page 9 of 58 The following table show the effect of tube length tube bore and viscosity on flow rate Effect of tube length Effect of tube internal Effect of viscosity on flow rate on flow rate diameter on flow rate Tube Tube length Flow rate Tube ID Flow Rate A om aro EC a 00 CU SES A ae e EN eee ee 3 2 General Theory When using the Mitos P Pump range it is important to be aware that the flow rate generated at a chosen pressure is dependent on the flow resistance of the system that you connect to and the viscosity of the fluid This is illustrated in the diagram below fluid flow Q
17. at PressurePumpDataLog Year Month_Date Time csv and files can be opened with Microsoft Excel MAR 000182_v 1 8 Page 39 of 58 10 11 Menu Option Screensaver The screensaver option allows the user to enter the screensaver mode manually In the main menu select option 6 to access the screensaver mode JRGE7 Screensaver mode Scrolls between a a current pressure and target pressure 10 12 Menu Option Information This menu option provides information on the software version and the current atmospheric chamber and supply pressure readings In the main menu select option 7 to access the information mode Scroll to view info Pressure readings update when scrolling Scroll MAR 000182_v 1 8 Page 40 of 58 11 PC Software User Control 11 1 Detect devices 11 1 1 USB connection Mitos P Pump and Mitos P Pump Remote pumps connect to your PC via a standard USB cable The pump will be automatically detected by the PC Software as soon as the PC Software has been started on the PC Users can also connect disconnect pumps after the PC Software has been started In that case the devices still get detected automatically but the detection might take up to 15 seconds 11 1 2 Connection v a a serial cable Mitos P Pump Basic and Mitos P Pump Remote Basic connect to your PC via a serial RS232 cable or USB to RS232 adapter cable 1 USB to RS232 cable If the pump is connected using Dolomite s USB to RS232 Adaptor
18. d by flow rates from other pumps It should be noted that if the main flow resistance is downstream of the junction then variations in the pressure of one pump will affect the flow rate from all other pumps In this situation the pumps can be described as dependent flow rate flow rate led varied constant system constant R 7 Independent Dependent MAR 000182_v 1 8 Page 46 of 58 13 Accessories Dolomite offers a broad range of accessories including flow resistors vessel holders and starter kits Product Name Product picture Advantages Mitos P Pump Vessel Holder Kit Part No 3200017 Flow Resistor F1 F3 F10 F30 F100 Part No 3200028 3200032 Mitos P Pump Starter Kit Part No 3200033 Pneumatic Connector Kit Part No 3200034 Mitos P Pump 3 way Chamber Lid Part No 3200044 MAR 000182_v 1 8 Enabling the Mitos P Pump range to be used with a wide range of standard and non standard fluid vessels The Flow Resistors can be used to insert additional flow resistance into a system Containing a selection of tubing and fittings to enable fluidic connection between a Mitos P Pump and a microfluidic system Containing a selection of fittings and tubing to enable connection of your compressed air or gas supply to a Mitos P Pump The Mitos P Pump 3 way Chamber Lid can be used to pump 3 liquids simultaneously from a Mitos P Pump Minimizes sample waste
19. e user dynamically controls the target using the twist and click control knob and the pump follows this moving target This is particularly useful for precise control of liquid movement on a microfluidic chip or for observing the effect of small pressure changes on a system e g droplet generation In the main menu select option 3 to access configuration options In the configuration menu select option 2 to access mode selection Scroll and click to select the required mode Scroll 10 7 Menu Option Configuration Resolution There are two resolution settings available The standard resolution setting allows the target pressure to be set in the smallest possible increment at low pressures but larger increments at higher pressures This enables the user to scroll through the pressure range quickly and efficiently The high resolution setting allows the target pressure to be set in the smallest possible increment across the entire pressure range This may be required where small pressure changes have a significant effect on a system However it will take a long time to scroll through the pressure range A n the main menu select option 3 PLOG to access configuration options In the configuration menu select option 3 to access resolution selection Scroll and click to select the required resolution Scroll MAR 000182_v 1 8 Page 37 of 58 10 8 Menu Option Configuration Calibration The calibration function
20. e to access very low flow rates and will not be able to adjust the flow rate in very small steps The benefit of a flow resistor is that the desired flow rate can be achieved by setting a pressure in the middle of the range This enables optimal flow rate control and resolution fluid flow Q P P Pump connected system RRs P 0 10 bar Rs is low Flow resistor required case 1 The second case is where 2 or more immiscible fluids of different viscosities are pumped into a junction and there is a length of channel flow resistance downstream of the junction The flow rate will be unstable because the viscosity downstream of the junction us alternates between low and high depending on whether fluid 1 or fluid 2 or both fluids are in the channel downstream However if correctly sized flow resistors are inserted between the Mitos P Pump range and the connected system then the flow rate can be stabilised This is because the flow rate is now determined by the R u in the flow resistor constant and the R u in the connected system varying Therefore for highly stable systems the flow resistance of the flow resistor Ri Riz should be significantly higher than the flow resistance of the connected system Rs low viscosity 8 fluid flow resistor P Pump Ru gt gt Rs P 0 10 bar flow rate would be unstable without flow resistors fluid flow Q Pi P2 Rit WitRs Us Rr2 Uu2 Rs Us connected sys
21. g the table below Desired flow rate at 1 bar pressure ul min 1 3 10 30 100 Flow rate Flow rate Flow rate Flow rate Flow rate range range range range range 0 01 10 0 03 30 0 1 100 0 3 300 1 1000 1 e g water at 20 C 10 25 wt CaCl sol at 20 C 30 vegetable oil at 40 C Dynamic viscosity of selected liquid cP 100 light machine oil at 20 C MAR 000182_v 1 8 Page 52 of 58 Why are the flow rates unstable in my system If you pumping 2 or more fluids into a junction and there is significant flow resistance downstream of the junction then a flow resistor may be required see section 10 1 Please contact Dolomite for further advice 15 Power consumption specification The maximum power consumption for the P Pump range is 40 Watts 1 67 Amps at 24 Volts MAR 000182_v 1 8 Page 53 of 58 The Dolomite Centre Ltd The Dolomite Centre Limited Unit 1 Anglian Business Park Orchard Road AOS sila 27 Jarman Way i loysto ordshire SG8 SHW UK Royston Hertfordshire SG8 STW UK ERP A AR Company No 05640084 T 44 0 1763 242491 F 44 0 1763 246125 W www dolomite centre com dolomite CE Declaration of Conformity In accordance with EN 45014 1998 We The Dolomite Centre Ltd of Unit 1 Anglian Business Park Orchard Road Royston Herts SG8 5TW UK declare that Equipment Mitos P Pump Model name Part number 3200016 in accordance with the following Direc
22. ing all Flow Sensors connected to the PC Software 3 Se a Devices B 4 amp Computer Connection Status Being Controlled Pump State 4 P Pumps lt g E La 100000 IDLE Pressure Control 4 100001 s LG16 0025 ES Flow Sensors Target 0 mbar 4 COM Ports a Chamber 0 mbar Y COM1 Version 1 0 13 z Supply 8000 mbar v Serial Number 100000 Flow Control E Flow Sensor ions os ee Type N ype is Current n a ul min Range n a Toolbox ba Sf bee he Connect flow sensor to control flow Toolbox icons Start Remote Control Stop Remote Control Tare Pump and Flow Sensor Tare Pump Tare Flow Sensor Erase chart data Save chart data to csv file Please note Some of the above icons might be hidden due to the type of pump you are using and the pump state When connecting the Mitos P Pump or Mitos P Pump Remote to the PC Software the Connection Status will automatically change to Being Controlled and the pump display will show PC CTL as shown below PC CTL PUMP 1 MAR 000182_v 1 8 Page 42 of 58 This will automatically disable the twist and click knob of the pumps and the pumps can only be controlled via the PC Software Change the PC control mode to Pump control mode To control the pump via the twist and click knob and display and use the PC Software for data log only select S Stop Remote Control The Connect
23. ing to the chip it is recommended to prime the inlet tubing This is achieved by flowing liquids through until they reach the edge connector and waiting until air bubbles have also been flushed through The Linear Connector can then be connected to the chip 4 Initially set all Mitos P Pumps to the same pressure e g 1bar Then gradually adjust the pressures of the pump channels to obtain the flow required MAR 000182_v 1 8 Page 51 of 58 How do I avoid gas dissolution in the liquid which is being pumped Some liquids can absorb air when put under pressure and then release the air in the form of bubbles when the pressure is reduced This occasionally causes problems with bubbles appearing after a flow resistor or a constriction To avoid this issue the following can be done e Ifthe system can be set up with less flow resistance and lower pressure then the level of gas dissolution can be reduced e The P Pump can be fed with pressurised Helium rather than pressurised air Helium has very low solubility in liquids in comparison to other gasses Please ensure the P Pump is used in a fume hood when operating with Helium e An immiscible liquid such as light oil can be dispensed onto the surface of the liquid that is being pumped to provide a barrier for gas dissolution How do select the correct flow resistor for my system The flow resistor should be selected based on the desired flow rate and the viscosity of the selected liquid usin
24. ion Status of the pump will change automatically from Being Controlled to Not Being Controlled Remote control can be enabled at any time by selecting nd Start Remote Control You can also exit the PC control mode via the pump as shown below PC CTL Press button to exit PC mode PUMP 1 Select Y for Yes and press button to confirm exit Please note The Mitos P Pump Basic and Mitos P Pump Remote Basic do not offer a twist and click knob for advanced control When connecting these pumps to the PC Software the Connection Status will always be Being Controlled The toolbox icons Start Remote Control and Stop Remote Control will be hidden 2 Logs This section displays all important events such as control commands sent to the pump pumps connection disconnection of a device or device errors 3 Help This section contains a link to Dolomite s website and allows users to access and download the Mitos P Pump datasheet MAR 000182_v 1 8 Page 43 of 58 12 Using Flow Resistors 12 1 Flow Resistors There are three main cases where it is necessary to insert a flow resistor between the Mitos P Pump range and the connected system The first case is where the flow resistance of the connected system is low large diameter short length of tube or channels As a result the user will have limited ability to pump at the low end of the flow rate range In practice this means that the user will not be abl
25. k on message to view more details Scroll Day and time are shown Warning message Scroll to read full message a Day and time are shown Error message SUPPLY Scroll to read full message MS Day and time are shown Information message HTING Scroll to read full message MAR 000182_v 1 8 Page 38 of 58 10 10 Menu Option USB Data Export The USB option allows pressure control data to be exported to a PC using a USB key Each time the Mitos P Pump and Mitos P Pump Remote are turned on a new data file is created This data file contains information on the atmospheric pressure mbar abs supply pressure mbar abs chamber pressure mbar gauge and target pressure mbar gauge If the pump is left running overnight a new data file is created at 00 00 00 The Mitos P Pump and Mitos P Pump Remote store data from the previous 7 days insert USB key Symbol indicates USB key is connected In the main menu select option 5 to access the USB menu Select COPY to transfer all data files to the USB key Please wait Message indicates that data SUCCESS transfer has been completed To safely dismount the USB key select REMOVE The USB can now be safely removed USB not connected In the main menu select option 5 to access the USB menu Indicates no USB key is connected Insert USB key and retry The pressure control data files are contained in a folder named logs The file names are in the form
26. ld be higher than the flow resistance of the connected system Rs _ _ gt high flow rate flow resistor eee R gt R P 0 10 bar fluid flow Q Pi Po A connected system Ro p1 Rs pis Rrz pl2 Rs ps back flow may occur without flow resistors flow resistor Re gt Rs P Pump P2 0 10 bar Flow resistor required case 3 Dolomite also offers a wide range of Mitos P Pump Flow Resistors Part No 3200028 3200032 to optimize your microfluidic system from a minimum flow rate of 0 01 l min to a maximum flow rate of 1000ul min For more information please visit our website http www dolomite microfluidics com webshop MAR 000182_v 1 8 Page 45 of 58 12 2 Flow Rate Calibration If the flow resistor resistance is much higher than the resistance of the connected system then the resistance of the connected system Rs Us can be ignored in determining the flow rate Therefore the flow rate from the pump is directly proportional to the pressure As a result the Mitos P Pump range can be calibrated for a specific fluid with the flow resistor before connecting to the system This is achieved by measuring the flow rate at a selected pressure Any change in the pressure will then give a predictable change in the flow rate e g if the pressure is increased by 20 the flow rate will be increased by 20 In this situation we can consider the pumps to be independent because they will not be affecte
27. liquid rates The high precision thermal sensor technology provides total media isolation and extremely low internal volume with no moving parts All measurement data is fully calibrated for water and temperature compensated by means of an internal microcontroller The Mitos Flow Rate Sensor simply attaches to the Mitos Sensor Display with a push click action Once connected to a Flow Rate Sensor the display can be used as a standalone device see 8 1 or sit on top of a Mitos P Pump see 8 2 By connecting the USB lead of the Sensor Display to a Mitos P Pump the display is powered and the Mitos P Pump records the flow rates which will be stored in a log file within the pump together with the pressure control data see 8 3 8 1 Standalone device Fig 1 Standalone device e Mitos Sensor Unit connected to a Mitos P Pump as a standalone device MAR 000182_v 1 8 Page 29 of 58 8 2 Connection to the top of the Mitos P Pump Connect tube from Mitos P Pump to Flow Sensor Connect mitos Lo de system Fig 2 Connection to top of a Mitos P Pump Fig 2 Connection to top of a Mitos P Pump e Connect Mitos Sensor Unit to the pins on e Connect tubing from a Mitos P Pump the Mitos P Pump through Mitos Sensor Unit and connect to your system 8 3 USB Connection Fig 3 USB Connection to the Mitos P Pump e use USB cable supplied with the Mitos Sensor Unit MAR 000182_v 1 8 Page 30 of 58 8 4 Electrical Connection To c
28. nnect their Mitos P Pump Basic or Mitos P Pump Remote Basic to a PC Page 48 of 58 13 1 Accessories compatibility Accessories Mitos Mitos P Pump Mitos P Pump Mitos P Pump P Pump Basic Remote Remote Basic Mitos P Pump Vessel Holder Kit Part No 3200017 Flow Resistor F1 F3 F10 F30 F100 Part No 3200028 3200032 Mitos P Pump Starter Kit Part No 3200033 Pneumatic Connector Kit Part No 3200034 Mitos P Pump 3 way Chamber Lid Part No 3200044 Mitos P Pump Remote Chamber 400 Part No 3200043 Mitos P Pump Remote Chamber 30 Part No 3200178 Dip Tube Fitting Part No 3200143 USB to RS232 Adapter Cable Part No 3200197 Mitos P Pump 3 way Vessel Holder Kit Part No 3200045 Y Accessories can only be used with the pumps when used with Mitos P Pump Remote Chamber 30 MAR 000182_v 1 8 Page 49 of 58 Why can t select a target pressure Please check you have connected a compressed air gas supply correctly see section 4 4 Why can t select a target pressure as high as my supply pressure To ensure quick response times the maximum target pressure available is limited to 250 mbar below supply pressure Why is the chamber pressure not reaching the target pressure If your target is near supply pressure then the cause may be fluctuation in your compressed air gas supply Please select a lower target or increase your supply pressure If you target is near atmosphe
29. nnection ON OFF switch from PC USB A connection x 2 for data download Flow Sensor Display Unit connection Not used MAR 000182_v 1 8 Page 21 of 58 The following accessories are supplied with the Mitos P Pump Remote e 2x Pneumatic supply cable e Tubing FEP 250um ID 1 16 OD 2m length e Power supply unit e Mains IEC lead User to supply the following e Vessel containing sample liquid o Liquids to be pumped must always be placed in a suitable vial within the glass chamber Never place any liquid directly in the glass chamber e Compressed air gas supply 6 2 Pneumatic Connection CONNECT TO PORT es To connect pneumatic supply To disconnect pneumatic supply use pneumatic supply cable included to link turn off compressed air gas supply first compressed air gas supply to the Mitos P Pump Remote push in sleeve of pressure supply connector to release either port can be used MAR 000182_v 1 8 Page 22 of 58 6 3 Power Connection PUSH IN TC Insert power cable Press switch to turn on e only use the power supply unit provided e 1 indicates ON and 0 indicates OFF 6 4 Connection to Remote Chamber The Mitos P Pump Remote features a pneumatic fitting which allows the connection to Dolomite s Remote Chambers or custom chambers As shown below the Mitos P Pump Remote is connected to the Mitos P Pump Remote Chamber 30 Part No 3200178 a glass pressure chamber which connects securely to the
30. ntrol current pressure in chamber relative to atmosphere status indicator default pressure target click to access menu reached wait 30 seconds timer mode E 1258mb section 3 2 target pressure click scroll click to select relative to atmosphere error click to display details Screensaver mode Scrolls between current pressure and target pressure The Mitos P Pump and Mitos P Pump Remote can operate with positive and negative supply pressure Maximum positive supply pressure is 11bar and this value should not be exceeded due to safety reasons There is no limit on the minimum supply pressure The range of the target pressure that can be set on the Mitos P Pump and Mitos P Pump Remote depends on the actual supply pressure For positive supply pressure the target pressure can be set within the range of MINIMUM PRESSURE 20mbars MAXIMUM PRESSURE SUPPLY PRESSURE 250mbars MAR 000182_v 1 8 Page 33 of 58 For example for the supply pressure of 3500mbars it gives the range of 20mbars to 3250mbars For negative supply pressure the target pressure can be set within the range of MINIMUM PRESSURE SUPPLY PRESSURE 80mbars MAXIMUM PRESSURE 20mbars For example for the supply pressure of 800mbars it gives the range of 720mbars to 20mbars Negative target pressure can be set within this range Positive target pressure can be set within this range Nega
31. ntrol over introduction of micro scale reagent volumes e Ideal for droplet generation higher long term droplet monodispersity in flow control mode compared with pressure control mode MAR 000182_v 1 8 Page 4 of 58 1 Important Information 6 2 Getting started 7 2 1 Introduction 7 3 Understanding Pressure Driven Flow 8 3 1 The Basics 8 3 2 General Theory 9 4 Mitos P Pump Part No 3200016 11 4 1 Features 11 4 2 Fluidic Connection 12 4 2 1 Opening Chamber 12 4 2 2 Inserting Fluid Vial 13 4 2 3 Closing Chamber 13 4 2 4 Adjusting Dip Tube 14 4 3 Pneumatic Connection 14 4 4 Power Connection 15 4 5 Mitos Sensor Unit Electrical Connection 15 5 Mitos P Pump Basic Part No 3200175 16 5 1 Features 16 5 3 Pneumatic Connection 17 5 4 Power Connection 18 5 5 USB to RS232 Connection 18 5 6 Mitos Sensor Unit Electrical Connection 19 6 Mitos P Pump Remote Part No 3200176 20 6 1 Features 20 6 2 Pneumatic Connection 21 6 3 Power Connection 22 6 4 Connection to Remote Chamber 22 6 5 Mitos Sensor Unit Electrical Connection 23 7 Mitos P Pump Remote Basic Part No 3200177 24 7 1 Features 24 7 2 Pneumatic Connection 25 Ta Power Connection 26 7 4 Connection to Remote Chamber 26 7 5 USB to RS232 Connection 27 7 6 Mitos Sensor Unit Electrical Connection 27 8 Mitos Sensor Units Connection 28 MAR 000182_v 1 8 Page 5 of 58 The Dolomite Centre Ltd 11 12 13 14 15 16 8 1 Standalone device 8 2
32. onnect to Mitos Sensor Unit e use electrical cable supplied with the Mitos Sensor Unit For more information on Dolomite s Mitos Sensor Units please visit our website www dolomite microfluidics com webshop MAR 000182_v 1 8 Page 31 of 58 9 Controlling the pumps The Mitos P Pump Part No 3200016 and Mitos P Pump Remote Part No 3200176 feature an intuitive twist and click knob and display for manual user control as described in 9 1 Additionally PC software is also available enabling the pumps to be controlled from a simple PC application see section 10 The Mitos P Pump Basic Part No 3200175 and Mitos P Pump Remote Basic Part No 3200177 can_only be controlled via the PC Software as described in section 11 Please contact our Technical Support Team support dolomite microfluidics com to receive a link to download the software online The pressure pump above features The pressure pump above features the twist and click knob and can be the basic design user control is only controlled via Manual User Control via PC Software as well as PC Software MAR 000182_v 1 8 Page 32 of 58 10 Manual User Control This section only applies to Mitos P Pump Part No 3200016 and Mitos P Pump Remote Part No 3200176 Manual Control Functions P Pump is starting Please wait P Pump is initialising Please wait Pressure control screen N A indicates that no target pressure has been selected 10 1 Pressure Co
33. onnection Insert RS232 connector e connect USB to PC The Mitos P Pump Basic connects to a PC via RS232 serial interface allowing data logging and control using free PC software For PCs that do not support an RS232 serial interface Dolomite offer a USB adapter cable Part No 3200197 MAR 000182_v 1 8 Page 19 of 58 5 5 Mitos Sensor Unit Electrical Connection To connect to Mitos Sensor Unit e The Mitos Sensor Units can also connect to the Mitos P Pump range via a fluidic connection for more information please see section 8 e use electrical cable supplied with the Mitos Sensor Unit For more information on Dolomite s Mitos Sensor Units please visit our website www dolomite microfluidics com webshop MAR 000182_v 1 8 Page 20 of 58 6 Mitos P Pump Remote Part No 3200176 The Mitos P Pump Remote provides pulseless liquid flow operating over a wide pressure range 0 10bar with excellent response time and accuracy Pressure driven flow is ideal for microfluidic systems where highly stable flow is required in the nl min to ul min range for applications such as droplet formation The design allows users to connect to Dolomite Remote Chambers with a push click action Flow sensor e module dock 6 1 Features Pneumatic fitting lt Twist and click control knob Display screen Cancel button Pressure supply connection Power supply i Mitos Sensor Interface connection connection USB B co
34. pump via a pneumatic fitting The Mitos P Pump Remote Chamber 30 can be placed on a hotplate magnetic stirrer or incubator to benefit applications requiring temperature control Connect Mitos P Pump Remote Chamber 30 Pneumatic tubing is connected to the pump and chamber via a push click action enabling quick release both ends and reducing downtime between experiments e connect via provided pneumatic tubing 3m length For information regarding fluidic connection to the Mitos P Pump Remote Chamber 30 please see 4 2 MAR 000182_v 1 8 Page 23 of 58 6 5 Mitos Sensor Unit Electrical Connection a m p Ag A Ryt Mae f ae A _ az ie A To connect to Mitos Sensor Unit e The Mitos Sensor Units can also connect to the Mitos P Pump range via a fluidic connection for more information please see section 8 e use electrical cable supplied with the Mitos Sensor Unit For more information on Dolomite s Mitos Sensor Units please visit our website www dolomite microfluidics com webshop MAR 000182_v 1 8 Page 24 of 58 7 Mitos P Pump Remote Basic Part No 3200177 The Mitos P Pump Remote Basic connects to a PC via RS232 serial interface allowing data logging and control using free PC software or your own applications A PC is required to use this pump For PCs that do not support an RS232 serial interface Dolomite offers a USB adapter Part No 3200197 All the other features of the pump are the
35. ric pressure then the cause may be an incorrect zero point Please use the tare function see section 10 4 Why is the chamber pressure not stabilizing If there is significant fluctuation in the supply pressure then it will be difficult for the chamber pressure to reach a stable value Also when very high flow rates are used the Mitos P Pump must constantly adjust the flow of air gas into the chamber and some instability may be unavoidable Can put my liquid directly in the chamber No You must always put the liquid to be pumped into a suitable vial and place this within the glass pressure chamber Why is the tare function not working Please ensure your compressed air gas supply has been disconnected If you have already disconnected your compressed air gas supply then air may be trapped in the pneumatic supply connector Please connect and disconnect the pneumatic supply cable to vent this trapped air MAR 000182_v 1 8 Page 50 of 58 Why is there pressure in the chamber when close the chamber lid The process of putting the chamber lid on compresses the air in the chamber slightly This can be avoided by ensuring that the dip tube fitting is not tightened until after the chamber lid has been closed Alternatively the chamber can be vented after the chamber lid has been closed by pressing the back button Why can t remove the chamber lid easily If the chamber is pressurised then the lid cannot be removed as
36. s into position MAR 000182_v 1 8 Page 14 of 58 Adjust dip tube Dip tube free unsafe Dip tube retained safe e ensure dip tube is below e inthis state the dip tube may to ensure safety retain dip surface of the liquid be ejected at high pressures tube by inserting into cleat 4 3 Pneumatic Connection CONNECTTOPORT 2 To connect pneumatic supply To disconnect pneumatic supply e use pneumatic supply cable included to link turn off compressed air gas supply first compressed air gas supply to the Mitos P Pump e push in sleeve of pressure supply connector to release MAR 000182_v 1 8 Page 15 of 58 4 4 Power Connection Insert power cable Press switch to turn on e only use the power supply unit provided e 1 indicates ON and 0 indicates OFF 4 5 Mitos Sensor Unit Electrical Connection To connect to Mitos Sensor Unit e The Mitos Sensor Units can also connect to the Mitos P Pump range via a fluidic connection for more information please see section 8 e use electrical cable supplied with the Mitos Sensor Unit For more information on Dolomite s Mitos Sensor Units please visit our website www dolomite microfluidics com webshop MAR 000182_v 1 8 Page 16 of 58 5 Mitos P Pump Basic Part No 3200175 The Mitos P Pump Basic connects to a PC via RS232 serial interface allowing data logging and control using free PC software or your own applications A PC is required to use this pump 5 1
37. should be used when the pressure control is more unstable than expected or takes longer than expected to reach the target pressure Over long periods of time there may be some degradation in the pressure control and the calibration function will retune the control for optimum performance The Mitos P Pump and Mitos P Pump Remote are factory calibrated to work best with compressed air If the pumps is to be used with a gas of significantly different density e g Helium then a calibration is recommended If the vessel size is changed to operate with the Mitos Remote Chamber 400 a calibration is also required Block liquid Before calibrating the liquid outlet port outlet port should be blocked to prevent liquid flow In the main menu select option 3 to access configuration options In the configuration menu select option 4 to perform a calibration Select Y to confirm and start the calibration Progress towards completion displayed The calibration takes approximately 20 min 10 9 Menu Option Error Log To aid troubleshooting and provide information on issues with the Mitos P Pump and Mitos P Pump Remote there is an error log In the error log all the previous messages for this session are recorded The 3 categories of logged messages are information messages warning messages and error messages In the main menu select option 4 Most recent message is to access the error log highest number in log Scroll and clic
38. tem high viscosity Rs downstream of Us varies depending on fluid junction produces whether fluid 1 or fluid 2 flow resistor flow instability or both fluids are in the P Pump channel downstream of Ri2 gt gt Rs the junction P2 0 10 bar Flow resistor required case 2 MAR 000182 v 1 8 Page 44 of 58 In applications such as droplet generation where fluid 2 is highly viscous it may not be possible to insert a large flow resistor on channel 2 since this will limit the flow rate range available to the user If a flow resistor is used the maximum pressure of 10bar may generate low flow rates but greater pressure would be required to access higher flow rates In this instance it is recommended that a flow resistor should only be inserted on channel 1 If highly stable flow is critical to the application it is also recommended that the connected system should be designed to have as low flow resistance as possible Please contact Dolomite for assistance The third case is where 2 or more fluids of similar viscosities are pumped into a junction and a high flow rate ratio is required 10 1 to 100 1 The problem is that there will be a tendency for back flow into the channel with the lower flow rate This can be resolved by inserting flow resistors between the Mitos P Pump range and the connected system to make flow through the connected system the path of least resistance Ideally the flow resistance of the flow resistor Ri Riz shou
39. the pressure locks the chamber Please vent the chamber before attempting to remove the lid If the chamber lid is difficult to turn then it is recommended to apply a small amount of silicone grease to the o ring inside the chamber lid For further advice on opening the chamber please see section 4 3 1 Will the flow rate obtained be affected by changes in atmospheric pressure No the Mitos P Pump range automatically adjusts the absolute pressure in the chamber to maintain a constant gauge pressure pressure relative to atmospheric pressure How do know what flow rate the fluid is flowing at Either a flow sensor is required or the system can be calibrated for a selected fluid so that a pressure can be translated into a flow rate Please contact Dolomite for further advice Why is there backflow into my Mitos P Pump When pumping 2 or more liquids using a Mitos P Pump there is the possibility of backflow from one pump at higher pressure to another pump at lower pressure This can be prevented by taking the following steps 1 Connect a flow resistor between the Mitos P Pump range and the chip for each liquid The flow resistor should be selected so that all liquids are flowing at approximately the same flow rate at a pressure of 1bar See flow resistor selection below 2 Ideally the flow resistance before the junction where 2 or more liquids meet should be greater than the flow resistance after the junction 3 Before connect
40. tive Atmospheric Positive Max supply pressure supply positive pressure pressure supply pressure 10 2 User Controls Back button Twist and click knob Press to stop pressure control press again to vent chamber NOTE chamber will also vent if the P Pump is switched off Scroll to menu option and press to access MAR 000182_v 1 8 Page 34 of 58 10 3 Menu Option Tare The tare option opens the pressure chamber to atmosphere and resets the zero point for the pressure in the chamber Once this has been set then the Mitos P Pump and Mitos P Pump Remote will maintain an accurate zero point for long periods of time The Mitos P Pump and Mitos P Pump Remote will automatically adjust to changes in atmospheric pressure so it is not necessary to use the tare function frequently disconnect pneumatic supply In the main menu select option 1 to reset the zero point Select Y to confirm and start the tare The process takes a few seconds Please wait This message indicates that the tare has been completed pneumatic supply still connected In the main menu select option 1 to reset the zero point Select Y to confirm and start the tare Message indicates that the pneumatic supply is still connected Disconnect and retry MAR 000182_v 1 8 Page 35 of 58 10 4 Menu Option Timer The timer option enables the pressure to be controlled at a selected value for a set period of time before venting to
41. tive s 73 23 EEC The Low Voltage Directive and its amending directives 89 336 EEC The Electromagnetic Compatibility Directive and its amending directives has been designed and manufactured to the following specifications EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements EN 61326 2 2 2006 Electrical equipment for measurement control and laboratory use EMC requirements EN 61000 4 3 2002 EMC Immunity EN 55022 2006 EMC Radiated current emissions hereby declare that the equipment named above has been designed to comply with the relevant sections of the above referenced specifications The unit complies with all Unit 1 Anglian Business Park Orchard Road Royston Herts SG8 5TW applicable essential requirements of the Directives UK on 8 March 2010 MAR 000182 v 1 8 Page 54 of 58 SNOT DYS arae CO ul O Name Andrew Lovatt Position CEO Done at The Dolomite Centre Ltd The Dolomite Centre Ltd The Dolomite Centre Limited Unit 1 Anglian Business Park Orchard Road Reo ees ar eN Royston Hertfordshire SG8 STW UK Royston Hertfordshire SG8 5HW UK Company No 05640084 T 44 0 1763 242491 F 44 0 1763 246125 W www dolomite centre com dolomite CE Declaration of Conformity In accordance with EN 45014 1998 We The Dolomite Centre Ltd of Unit 1 Anglian Business Park Orchard Road Royston Herts SG
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