data sheet
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1. 40 to 50 C Meets CE requirements CE models only WARRANTY Next Advance warrants its Products against defects in materials and workmanship for time periods which vary according to the Product Within these time periods Next Advance will replace or repair without charge to the original purchaser any part which is defective Bullet Blender Two years The warranty is void if the Product is defective due to product accident product NEXT gt gt gt ADVANCE 6 OPERATOR S MANUAL FOR THE BULLET BLENDER modification exposure to radiation other than for sterilization connection to an improper electrical supply lack of proper maintenance contamination improper installation or misuse If the product is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired The warranty shall also not apply to defects arising from fire flood lightning or other conditions unrelated to correct operation of the Product Next Advance s liability is limited at the company s election to 1 refund of the original purchaser s purchase price for the Product 2 repair of the Product or 3 replacement of the Product or defective parts Evidence of purchase by the original purchaser is required Next Advance may also request documentation of proper maintenance if applicable Next Advance makes no other warranty express or implied with respect to its Products NEXT ADVANCE2. BULLET BLENDER DISCLAIMER Next Advance Inc makes no representations or warranties expressed statutory or implied regarding the fitness or merchantability of this product for any particular purpose In no event shall Next Advance be liable for incidental or consequential damages Next Advance Inc is not liable for any damages including but not limited to lost profits lost savings or other incidental or consequential damages arising from ownership or use of this product or for any delay in the performance of its obligations under the warranty due to causes beyond its control Next Advance Inc also reserves the right to make any improvements or modifications to the product described in this manual at any time without notice of these changes Next Advance Inc products are not designed intended or authorized for use in applications or as system components intended to support or sustain human life as a clinical medical device for humans or for any application in which the failure of the product could create a situation where personal injury or death may occur All brand and product names used in this manual are the trademarks of their respective Owners CONTACT INFO Next Advance Inc Averill Park NY USA Telephone 518 674 3510 www nextadvance com Email info nextadvance com techsupport nextadvance com sales nextadvance com NEXT gt gt gt ADVANCE
3. MAKES NO WARRANTY RESPECTING THE MERCHANTABILITY OF THE PRODUCTS OR THEIR SUITABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE OR USE Next Advance shall not be liable for indirect special incidental or consequential damages of any nature Any recovery for any claim shall be limited to the original purchase price for the product Operator s Responsibility Provide proof of purchase and provide normal care and maintenance WARNINGS AND CAUTIONS Read the user s manual before operating Do not operate Bullet Blender 5 units with fewer than 3 tubes Do not operate Bullet Blender units with empty tubes Do not open lid when Bullet Blender is in use Do not insert fingers or objects other than recommended tubes into sample tube holes Use caution when closing Bullet Blender lid do not close on fingers Use recommended tubes only No user serviceable parts are inside For indoor use only Pollution Degree 2 per EN 61010 1 Overvoltage Category II per EN 61010 1 Enclosure Protection Not Protected Against the Ingress of Moisture Sound Pressure Level up to 90dBA for BBX24 models up to 100dBA for BBY24M and BBX5MB models Use protective devices that reduce exposure to hearing below 85 dBA during prolonged exposure Do not immerse in liquid Before touching the Bullet Blender touch a bare metal surface to discharge static electricity NEXT gt gt gt ADVANCE 7 OPERATOR S MANUAL FOR THE
4. OPERATOR S MANUAL FOR THE BULLET BLENDER Operator s Manual The Bullet Blender Models Bullet Blender Bullet Blender Storm Bullet Blender Blue and Bullet Blender Blue 5 Congratulations Congratulations on your purchase of a Contents Bullet Blender by Next Advance Inc for mixing lysing disrupting and homogenizing your samples Please read this operator s manual which explains proper operation of the instrument This manual is posted on our website www nextadvance com Click on SUPPORT in the left window and then on the appropriate link to the manual We re confident that your Bullet Blender will become an essential tool in your laboratory and we wish you success with your work BBmanual_CE_v121111 NEXT gt gt gt ADVANCE 1 OPERATOR S MANUAL FOR THE BULLET BLENDER Parts of the Bullet Blender Cover Gasket Sample PA Tubes Sample Plate Enclosure Operator Panel NEXT gt gt gt ADVANCE OPERATOR S MANUAL FOR THE BULLET BLENDER SYMBOLS USED ON THE BBX AND BBY MODEL BULLET BLENDERS D Start Operation Caution Follow the Instructions in the Operator Manual C 4 This product complies with the European Low Voltage and EMC Directives Z4 Please dispose the test tubes and the BBX or BBY Bullet Blender in accordance with local regulations NEXT gt gt gt ADVANCE OPERATOR S MANUAL FOR THE BULLET BLENDER SETUP Place the Bullet Blender on a
5. PLE SETTINGS The following ratio should be used as a guideline for determining the amount of beads and buffer to use given a certain sample size 1 volume mass of tissue 1 2 volume of beads 2 volumes of buffer For more specific information regarding the use of various beads as well as specific protocol information please refer to our website www nextadvance com As the tissue amount becomes smaller the above recommended ratio may differ due to the limitations of handling of the small volumes With microcentrifuge tubes we recommend using a minimum of 25uL of buffer regardless of your sample size For the 5mL tubes we recommend a minimum volume of 100uL With microcentrifuge tubes the maximum sample mass is 300mg of tissue or 300uUL of pelleted cell culture per microcentrifuge in the Bullet Blender and the tube should not be filled more than two thirds of the way this includes sample beads and buffer This is homogenization require empty space in the because the mechanics of tube For 5mL tubes do not operate with more than 1g of sample or a total of 3mL of buffer tissue and beads per tube Cutting the tissue into smaller pieces will generally yield better results Tissue with a high aspect ratio long thin strips will homogenize better than tissue that is round or cubic Do not operate the Bullet Blender using the same tubes for longer than 15 minutes Protocols for many types of sampl
6. es are posted on our website at www nextadvance com FAQs protocols htm Notes Use high quality polypropylene snap top tubes such as Eppendorf Safe Lock microtubes The Bullet Blender Blue 5 is designed to work with 5mL Axygen brand tubes only At high speed settings there may be some flaking of the tubes This is a normal side effect of homogenization The higher speed enables homogenization of tougher tissue CLEANING If you wish to clean your Bullet Blender clean the outside of the unit only with mild soap water and a soft cloth Under normal conditions the Bullet Blender should never need to be disassembled for cleaning In the case of a large spill unplug the instrument remove the sample tube plate with an 1 8 hex wrench wipe out the spill using standard laboratory safety precautions and replace the sample tube plate Do not touch or tamper with the electronics NEXT gt gt gt ADVANCE 5 OPERATOR S MANUAL FOR THE BULLET BLENDER TROUBLESHOOTING In addition to the tips given below a thorough list of troubleshooting tips is at http www nextadvance com FAQs FAQs Bullet_Blender htm If the Bullet Blender doesn t start the plug of the power supply cord might not be in a live wall outlet or the power supply connector might not be fully inserted in the AC Input Receptacle of the Bullet Blender For Bullet Blender 5 units Make sure the Power Switch is in the ON I position If the
7. led partially with water For best results the tubes should be evenly spaced Set the duration in minutes and the speed to the desired value Push the start button As each minute passes another LED On the BLUE and BBY models the air cooling will continue for half will light up a minute post homogenization Do not operate with the cover open There will be excess noise your samples will not be properly processed and the tubes may be knocked entirely out of the instrument potentially causing injury If you turn the minutes knob to 0 the instrument will stop Example The figure above shows the Bullet Blender set to run for 4 minutes at a speed of 8 after running for 2 minutes To operate press the START button After the first minute the LED light by the number 1 will light up After the second minute the second LED light by the number 2 will light up And so on At the settings shown above after 4 minutes the Bullet Blender will stop In the Blue versions the words Air Cooling will light up in blue during NEXT gt gt gt ADVANCE 4 OPERATOR S MANUAL FOR THE BULLET BLENDER operation and for about one half minute afterward as the fan continues to operate Note that the Bullet Blender Storm models have additional time settings for 15 and 30 second pulses In addition the Bullet Blender powerful speed setting of 12 Storm has additional more PROTOCOLS AND SAM
8. stable level lab bench Plug the AC power supply cord connector into the AC Input Receptacle on the back of the Bullet Blender and then insert the plug into a wall outlet You should see lights on the time setting flash for several seconds indicating that the unit is receiving power It is now set up OPERATION To use your BBX or BBY series Bullet Blender appropriate tubes and close the cover For BBX24 models BBX24 and BBX24B place up to 24 1 5mL to 2 0mL polypropylene high quality snap top e g Safe Lock tubes containing samples into the sample lift open the cover insert the closed Eppendorf microcentrifuge plate and close the cover When using 2 0mL tubes we recommend using at least 2 tubes per homogenization cycle For the screw cap BBX24 models BBX24W and BBX24BW use 1 5ml polypropylene Corning or Axygen tubes conical other tubes may work but we can not assure good results with them For the Bullet Blender Storm BBY24M use 1 5mL Eppendorf Safe Lock tubes In the Bullet Blender Blue 5 BBXSMB load up to 12 SmL Axygen brand transport tubes The snap caps on the microcentrifuge tubes must be on securely The threads on all screw cap tubes must be dry and the caps must be screwed on very tightly Do not operate a Bullet Blender loaded with any empty BBmanual_CE_ v121111 tubes as this may result in breakage of the tubes If their use is necessary blank tubes should be fil
9. unit stops working turn the system off for 15 minutes to allow the electronics to reset If the Bullet Blender does not turn on after this period contact customer service If the caps on the microcentrifuge tubes pop open or the caps on 5 mL tubes loosen make sure that the interface regions or screw threads between the lids and the caps is dry when you close the caps or screw them on so that there is enough friction for the caps to remain tight Using recommended types of tubes will minimize cap failure SUPPORT FAQs protocols and other helpful information is available on our website http www nextadvance com Click on the Bullet Blender then on the appropriate link If you cannot find an answer there please contact customer service by email at support nextadvance com or by telephone at 1 518 674 3510 or 800 738 1681 SPECIFICATIONS Size 23 cm 9 in deep x 20 cm 8 in wide x 22 cm 8 5 in high Weight 4 to 5 kg 8 5 to 11 lbs depending upon model Power Requirement BBX24 BBX24W BBX24B and BBX24BW models 24 VDC 0 75 Amp BBY24M and BBXS5MB models 24 VDC 2 5 Amp Capacity 24 of 1 5 2 0mL poly propylene high quality e g Eppendorf sample tubes 24 of 1 5 Axygen brand 1 5mL conical screw cap tubes or 12 of 5mL Axygen brand 5ml transport tubes depending upon model Relative Humidity 5 90 non condensing Operating Temperature 4 40 C Altitude lt 2000m Storage Temperature
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