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HM 550 Microtome - biology tech support page

1. REM TRV 23000 CONTIN FINE 3 Fig 33 SPECIM 25 24 NJ CHAMBER 20 21 ME TIME INE 3 TRIM 20 xxx REN R Fig 34 MICRON 2 10 SETTING THE REAL TIME WAKE TIME ACTIVE TIME AND DATE 2 10 1 SETTING THE REAL TIME Press the menu button fig 38 9 This way you get into the list of the submenus Select Real Time via the arrow buttons fig 37 5 and 37 6 This submenu is now shown inverted Mia the enter button fig 38 10 confirm this submenu and simultaneously open it 270 change the instrument s real time at intervals of one minute press the arrow buttons fig 37 5 or 37 6 If the buttons are being pressed for more than 5 seconds the time changing will be accelerated Confirm via enter button fig 38 10 Note You return immediately to the list of submenus If the setting is not confirmed via the enter button you will quit the submenu automatically after five seconds and you return into the list of submenus The current setting of the submenu is also chosen and will still be valid To quit the submenu press the menu button fig 38 9 Now continue working with the selected settings MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Vers 1 00 DEFROST TIME WAKE TIME STANDBY DELAY REAL TIME DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COARSE FILT RESET RETRACTION ENABLE
2. Make sure that the section thickness setting is selected via the button fig 26 4 Section thickness is shown inverted on the display Press the arrow buttons fig 26 5 or 26 6 to select the desired value Turn the handwheel in a clockwise direction to feed the specimen at the selected section thickness Note If the fine feed is carried out via the motorized drive please read part 2 18 option M MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 26 MICRON 2 8 TURNING ON OFF THE FUNCTION RETRACTION If needed the function retraction can be turned off Press the menu button fig 29 9 This way you get into the list of submenus Mia the arrow buttons fig 28 5 and 28 6 select the submenu retraction ON OFF The selected submenu is now shown inverted Mia the enter button fig 29 10 confirm the submenu and simultaneously open it Mia the arrow buttons fig 28 5 or 28 6 select the desired setting ON or OFF Tonfirm the setting via the enter button fig 29 10 Note You return immediately to the list of submenus fig 27 If the setting is not confirmed via the enter button you will quit the submenu automatically after five seconds and you return into the list of submenus The current setting of the submenu is also chosen and will still be valid To quit the submenu press the menu button fig 29 9 Now continue working wit
3. 7he controlled cutting speed can be set continuously from 0 up to 250 mm s The cutting speed refers to the cutting window see part 2 18 1 For the upward return travel a proportionally higher retraction speed is carried out To adjust the cutting window in relation to a specimen it can be set continuously within the maximum values 60 mm and can be adjusted to the respective specimen 2 18 1 SETTING THE CUTTING WINDOW The size of this zone is shown in millimeters within the symbol for the cutting window When passing the cutting window this symbol is shown inverted on the display It is shown on the display fig 82 in the status line with the letters AW automatic window Note Cutting and suction window are identical MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf SPECIM CHAMBER COUNTER FINE 3 225 20 0 HM 550 Series az 21 E Fig 82 MICRON Manual setting 7he cutting window is determined by manual entries For this turn the handwheel so that the lower edge of the specimen is positioned slightly above the knife edge Press button fig 83 1 to set the upper limit of the cutting window Tontinue turning the handwheel clockwise to place the upper edge of the specimen just below the knife edge Press button fig 83 1 to set the lower limit of the cutting window 7he LED in button fig 83 1 lights up during each further passing
4. freezer burns on frozen parts and potential contaminated material it should only be done by skilled or trained personnel Please proceed as follows Wear protective gloves Remove knife blade from the knife carrier and store it in a knife case Remove the brush shelf tools as well as chucks Bring the handwheel handle into its upper position i e the specimen clamping is alsin in its upper position Remove the middle and rear section waste tray Dispose of the section waste according to the respective lab regulations Remove and or suck off cold section waste If the instrument is equipped with the option Vacutome pull off the Vacutome connection on the right side of the knife carrier and insert the cleaning hose on the Vacutome connection Turn off the mains switch and unplug the unit When equipped with the option Vacutome remove the coarse filter and if necessary the fine filter and dispose of according to the applicable lab regulations Loosen the clamping lever on the knife carrier and pull off the knife carrier If necessary treat it with disinfectant Caution Caution of freezer burns due to the frozen knife carrier MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series Remove the left and right side sheet For this turn the knurled screws approx two rotations Remove the sheets on the black grips with a circular movement upw
5. 2 24 2 Additional equipment MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series PART 3 Theory of operation 3 1 Microtome cooling defrost cycle 3 2 Cutting movement 3 3 Specimen coarse feed and trimming stages 3 4 Automatic approach system 3 5 Option M motorized cutting drive 3 6 Option V stretching sections and disposing of section waste 3 7 Option O Specimen fast and temperature control 3 8 Option D Integrated automatic unit for the application of disinfection media PART 4 Working with the cryostat 4 1 Preparations on the microtome and inside the cooling chamber 4 2 Freezing on the specimen 4 3 Orientation and trimming of specimens 4 4 Sectioning and taking off sections 4 5 How to avoid malfunctions 4 5 1 Possible sources of errors cause and removal PART 5 Maintenance and care of the cryostat 5 1 Shutting off for cleaning 5 2 Removing the microtome 5 3 Cleaning and care of the microtome 5 4 Cleaning the cooling lamella 5 5 Changing the fluorescent lamp PART 6 CONDITIONS FOR THE TRANSPORTATION OF THE INSTRUMENT 6 1 Taking back the instrument for repair or routine maintenance 6 2 Disposal of the instrument after final shutdown MICROM International GmbH Robert Bosch Sir 49 D 69190 Walldorf MICRON HM 550 Series EC Certificate of Conformity Name and address of MICROM International GmbH the manufacturer Robert Bosch Strafse 49 D 69190 Walldorf Product design
6. 2 7 3 SETTING THE CUTTING WINDOW To activate an automatic approach process it is necessary to set a cutting window first The size of this window is then shown in the symbol cutting window When passing the cutting window this symbol is shown inverted on the display During this automatic function the cutting window adjusts itself to the size of the specimen Note The automatic window is only possible within the temperature range of 5 C and 35 C Beyond these temperature limits the automatic window turns off automatically and the manual setting is possible only Manual setting df necessary the cutting window can be determined by manual entries For this turn off the automatic cutting window registration As a precondition for this process values must be shown in the info line For this press button fig 23 8 and while this button fig 23 8 is still being pressed press button fig 25 1 The letters AW disappear from the display 7 urn the handwheel so that the lower edge of the specimen is positioned slightly above the knife edge Press button fig 25 1 to set the upper limit of the cutting window Continue turning the handwheel clockwise to place the upper edge of the specimen just below the knife edge Press button fig 25 1 to set the lower limit of the cutting window 7he LED in button fig 25 1 lights up during each further passing through of the cutting window The lengt
7. that has been selected before will be used again MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Ver 2 00 OPTION D ON OFF MODE OPTION D eee Leer WI ES NO PISINEEOT MEDIUM DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COARSE FILT RESET RACTION ENABLE LANGUAGE Fig 114 5 6 Fig 115 Fig 116 OPTION D Choose UP amp DWN confirm ENTER Fig 117 MICRON MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Submenu Mode Option D Press the enter button fig 116 10 to select the submenu Select between weekly daily or immediately via the arrow buttons fig 115 5 and 115 6 Confirm with the enter button fig 116 10 Mode immediate immediat In case this mode fig 119 has been selected the cycle is immediately started after having pressed the enter button fig 116 10 and when quitting the menu Warning The automatic cycle starts after the sliding window has been closed The immediate cycle is carried out only once In case another mode daily or weekly has been set this mode remains active after having finished an immediate mode If the immediate start coincides with the previously selected mode daily or weekly an immediate start is carried out and the daily and or weekly one is not started on that day Mode every day
8. Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 12 SPECIM 25 42 Zz CHAMBER 20 21 BME LOCKED COUNTER 0O CONTIN Fig 13 MICRON 2 4 CUTTING PROCESS 2 4 1 CUTTING MOVEMENT AND RETRACTION Turn the handwheel in a clockwise direction so the cutting movement is carried out As the specimen moves down sections are produced Tontinue turning the handwheel clockwise to bring the specimen back up 270 protect the knife and specimen during return travel the specimen clamping is retracted electro mechanically This is shown by the letter R in the status line on the display Warning When the motor drive option M for the cutting movement has been turned on the same run process as above mentioned is carried out Keep the rotating area of the handwheel clear Keep enough distance to the rotating handwheel handle MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series X selected section thickness 1 specimen 2 cutting movement 3 knife 4 return travel R retraction Iie R ith No Fig 14 MICRON 2 5 SETTING SECTION THICKNESS AND TRIMMING THICKNESS HM 550 Series i i SPECIM 25 2 i Basic status of the operating control CHAMBER 20 21 MENN 2770 choose between section thickness and trimming thickness press button fig 16 4 COUNTER 0 The selected setting is shown inverted on the display fig 15 7hen enter the desire
9. Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 2 7 2 SPEED FOR SPECIMEN COARSE FEED The speed for the specimen coarse feed can be selected in three different settings The selected setting is shown in the status line on the display by one two or three little stars Three stars means the fastest coarse feed setting Setting the speed Press the menu button fig 23 9 This way you will get into the list with the submenus Select coarse feed via the arrow buttons fig 22 5 and 22 6 The selected submenu is now shown inverted Press the enter button fig 23 10 to confirm the submenu and open it Via the arrow buttons fig 22 5 and 22 6 kk choose between or Note One star means a slow approach speed two stars mean a medium speed and three stars stand for a fast approach speed Press the enter button fig 23 10 to confirm the setting Note You return immediately to the list of submenus If the setting is not confirmed via the enter button you will quit the submenu automatically after five seconds and you return into the list of submenus The current setting of the submenu is also chosen and will still be valid To quit the submenu press the menu button fig 23 9 Now continue working with the selected settings MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 22 Fig 23 MICRON
10. TECHNICAL DATA HM 550 SERIES 45 dB A measured with 1 m distance to the instrument cooling agent R404a filling amount 230 g 230 V 50 Hz R404a filling amount 250 g 115 V 60 Hz Wide w o handwheel 640 mm deep 760 mm high 1200 mm 125 155 kg depending on the model All temperatures refer to an ambient temperature of 20 C MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series 1 On indication sleep status 2 Button specimen temperature Standard version not used as there is no specimen cooling 3 Button chamber temperature 4 Button TRIM FEED 5 Arrow button UP Note some of the buttons might be disabled MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf Fig 2 6 Arrow button DOWN 7 LC display 8 Reset button 9 Menu button 10 enter button within the menu settings scroll button outside the menu settings This instruction manual includes all options If your instrument is not equipped with all options MICRON HM 550 Series Fig 3 1 Button cutting window 2 Button ACA 3 Button coarse feed forwards 4 Button coarse feed backwards 5 Button TRIM MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON K 1 Button operating modes 2 Operating knob 3 Button START STOP 4 Button START STOP 5 Emergency stop LED 6 Button to activate the handwheel
11. The selected clearance angle is thus fixed in its position Note Usable cuts are only achieved at a clearance angle of 10 or more Moving the blade carrier To move the blade carrier loosen the lever on the right side of the base plate To fix the blade carrier in its new position clamp the lever again Fine adjustment of the vacuum anti roll hood The vacuum anti roll hood fig 101 4 can be removed and put on by using the turning lever fig 101 5 The knurled screw fig 101 6 allows the adjustment of the edge of the vacuum anti roll hood fig 101 4 in relation to the blade edge The fine adjustment of the anti roll device is carried out via the knurled screw fig 101 6 Function of the Vacutome A closed vacuum anti roll hood fig 102 4 and a suitable suction vacuum generate stretching or suction effects For this the closed vacuum anti roll hood fig 102 4 slightly tilts the underneath flap fig 102 6 thus opening a suction slot fig 102 7 Lift the vacuum anti roll hood fig 102 4 the flap fig 102 6 beneath falls back in place and the slot gets closed MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON HM 550 Series 2 20 2 MAGNETIC BLADE CARRIER MV The magnetic blade carrier is suitable to take up MICROM blades with the dimensions 60 x 19 mm anda facette angle of approx 33 Fig 103 Fig 104 MIC
12. fig 96 1 and remove the micro filter Dispose of the micro filter according to the respective lab regulations Note The micro filter is to be understood as a disposable filter and must not be used again Inserting the micro filter Connect the filter again with the connecting pieces Note When inserting the filter please note the correct direction fig 96 4 The arrow on the micro filter must show upwards The internal counter for the micro filter must now be reset For his press the menu button fig 98 9 This way you get into the list of submenus Via the arrow buttons fig 97 5 and 97 6 select micro filter This submenu is now shown inverted Via the enter button fig 98 10 confirm this setting and simultaneously open it Via the arrow buttons fig 97 5 and 97 6 reset the sum of sections since the last resetting to 0 um Via the enter button fig 98 10 confirm this setting MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 96 Fig 97 MICRON Note You return immediately to the list of submenus lf the setting is not confirmed via the enter button you will quit the submenu automatically after five seconds and you return into the list of submenus The current setting of the submenu is also chosen and will still be valid To quit the submenu press the menu button fig 98 9 Now continue working w
13. order as well as the corresponding insertion of the connectors must be striclty observed for the option O MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series Bring the handwheel handle into its lower position i e the specimen clamping is also in its lower position Slightly lift the heated window on the handle and pull it forwards over the front arm rest Then swivel the window upwards and slightly move it backwards Then insert it into the anchorage of the guide rails fig 131 Unscrew the Allan screw fig 129 1 in the middle of the front part of the microtome base plate via an Allan screwdrive size 5 mm Caution AN It is recommended for all further work to wear isolating gloves because of the danger of freezer burns on the frozen microtome Move the microtome to the left side until the stop This way the clutch on the right side of the microtome becomes free 2 Move the microtome forwards and remove it from the cryo chamber Thoroughly clean and wash the interior Caution Chloric cleaning agents must not be used for cleaning purposes In case of a malfunction hazardous gases might be generated together with the cooling brine Tyfoxit of the cooling system Fig 132 Pull off the red stopper at the bottom of the chamber The cleaning liquid is collected in the container fig 182 1 with a volume of 4 8 at the front lower side Note Plea
14. plate Afterwards tighten the clamping screws fig 75 2 to fix the knife in its position Fine adjustment of the anti roll device The fine adjustment of the anti roll device is carried out via the knurled screw fig 75 6 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON Selecting the clearance angle Loosen the clamping lever fig 75 8 on the left side of the knife carrier Swivel the upper part of the knife carrier on the base until the desired clearance angle is reached The clearance angle can be read on the scale fig 75 9 on the left side on the base Bring the clamping lever fig 75 8 upwards into clamping position The selected clearance angle is now fixed in its position Note Usable cuts are only achieved at a clearance angle of 10 or more MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON HM 550 Series 2 16 2 DISPOSABLE BLADE CARRIER EC The disposable blade carrier EC takes up all commercially available low profile blades with a dimension of 80 x 8 mm and a facette angle of approx 35 and as another version it takes also up high profile blades Fig 76 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON Inserting the blade The blade is inserted into the slot behind the clamping plate Note When using high profile blades first loosen the lever f
15. 69190 Walldorf HM 550 Series MICROM GmbH 69190 Walldorf Germany Type HM 550_ Ser No _ SAPs Ns Chamber Refr R 404a g Max Press 1900kPa Made in Germany IVD Fig 8 MICRON Note To avoid frost built up on the specimen the specimen temperature is controlled to a temperature of 10 C until the knife reached a temperature of The cooling phase will take approx 2 to 3 5 h depending on the set chamber temperature between 15 C and 30 C MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 2 3 BASIC OPERATIONAL RULES When turning on the instrument the display shows the configuration of the instrument e g the figures show HM 550 MV the section thickness selection the specimen and chamber temperatures with the corresponding set and actual values the symbol for the cutting window This is the display fig 9 while the instrument is in its active status The WAKE time is used to trigger the instrument back into the active state It should be set with sufficient time before work is started approx 1 5 h To save energy and extend the lifetime of some instrument components the instrument has two more operative status Standby status Sleep status The instrument switches to the standby status fig 10 when it is turned on but not operated for more than a pre selected active time see part 210 3 between 1 and 9 h The
16. LANGUAGE ILLUMINATION Fig 35 REAL TIME Choose UP amp DWN Confirm ENTER Fig 36 Fig 37 Fig 38 MICRON 2 10 2 SETTING THE WAKE TIME The WAKE time is used to trigger the instrument back into the active state It should be set with sufficient time before work is started approx 1 5 h Press the menu button fig 42 9 This way you get into the list of submenus Via the arrow buttons fig 41 5 and 41 6 select Wake Time This submenu is now shown inverted Via the enter button fig 42 10 confirm this submenu and simultaneously open it To change the WAKE time at intervals of 15 minutes press the arrow buttons fig 41 5 or 41 6 Confirm with enter fig 42 10 Note You return immediately to the list of submenus If the setting is not confirmed via the enter button you will quit the submenu automatically after five seconds and you return into the list of submenus The current setting of the submenu is also chosen and will still be valid To quit the submenu press the menu button fig 42 9 Now continue working with the selected settings After having reached the WAKE time the instrument switches over to the active status MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Vers 1 00 DEFROST TIME WAKE TIME STANDBY DELAY REAL TIME DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FI
17. Menu service settings are only PeCOESTBEEROST relevant for service technicians INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COARSE FILT RESET RETRACTION ENABLE LANGUAGE ILLUMINATION SERVICE Fig 73 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 15 SPECIMEN ORIENTATION In many cases the orientation of the specimen in relation to the cutting edge would be advantageous This can easily be done by means of the orienting specimen holder on the microtome Loosen the clamping lever fig 74 7 Via the orienting lever fig 74 6 the specimen clamping can be moved in all directions by approx 8 After having achieved the desired alignment fix the position via the clamping lever fig 74 7 The orienting lever fig 74 6 is also used for clamping the chuck into the orienting specimen holder Note The specimen orientation includes a zero device for the specimen chuck is always parallel in relation to the knife Zero position can be felt noticeably MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 74 MICRON HM 550 Series 2 16 KNIFE CARRIERS 2 16 1 STANDARD KNIFE CARRIER The standard knife carrier of the cryostat is easy to use The knife can be inserted either from the side or from the front The standard knife holder takes up commercially available conventional knives with c and d prof
18. Series Fine adjustment of the anti roll device The fine adjustment of the anti roll device is carried out via the knurled screw fig 67 6 Selecting the clearance angle Loosen the clamping lever fig 76 3 Swivel the upper part of the blade carrier on the base until the desired clearance angle is reached The clearance angle can be read on the scale fig 76 4 on the left side on the base Bring the clamping lever fig 76 3 into clamping position The selected clearance angle is now fixed in its position Note Usable cuts are only achieved at a clearance angle of 10 or more MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series 2 16 3 MAGNETIC DISPOSABLE BLADE CARRIER M The magnetic disposable blade carrier takes up MICROM blades with the dimensions 80 x 19 mm and or 60x19 mm and a facette angle between 24 and 30 Fig 77 Fig 78 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON Note Special MICROM magnetic blades must be used only Inserting the blade Insert the blade into the blade slider fig 78 1 and put it onto the magnetic surface fig 78 2 of the blade carrier from the side Note Always insert the blade in a way that the large facette of the blade is inserted downwards So it is invisible for the user When inserting the blade the blade slider together with the bla
19. again shown inverted MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series veecunvi rev ICHAMBER 20 COUNTER 0O CONTIN TRIM 20 Fig 17 Fig 18 MICRON 2 6 2 FAST FREEZING FOR STANDARD INSTRUMENTS There are several possibilities to freeze on specimens Various specimen chucks are available Round specimen chucks can be supplied with a diameter of 30 mm and 40 mm and rectangular specimen chucks with a size of 50 55 60 and 70 mm Special sizes on request 2 2 2 Put the warm chuck into the hole fig 19 1 of the fast freezing rail Put freezing medium onto the chuck Place fresh tissue onto the still liquid freezing medium When the medium and tissue are frozen the chuck can be inserted into the specimen clamping and can be fixed The specimen can now be sectioned Note The upper side of the freezing station must not be covered by frost or ice To avoid this use ethanol absolute or the like Note If the instrument is equipped with the option P active deep freezing device with Peltier element please also see part 2 21 Option P MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series SS GPP a G Fig 19 MICRON 2 6 3 HEAT EXTRACTOR When using a heat extractor in combination with the so called cryo molds the specimen is frozen faster and more evenly as the specimen is f
20. and reaches the area of the suction slot From here the section gets through the blade carrier and the tubings into the filter unit Section waste is collected in the coarse filter which can easily be replaced It must be disposed of according to the respective lab regulations The air of the suction flow now passes the micro filter and is filtered there Afterwards the filtered air flows back into the rear area of the cryochamber MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON HM 550 Series 3 7 OPTION O SPECIMEN FAST AND TEMPERATURE CONTROL The specimen fast cooling and temperature control serves for a more efficient working with the cryostat For various specimens different cutting temperatures can be achieved very quickly There is the possibility to rapidly freeze on the specimen at 50 C on a chuck with the freezing medium However the freezing on can also be carried out on the fast freezing stations or with the Peltier cooling element To bring the specimen to the desired cutting temperature switch over to temperature control The set value of the cutting temperature can be selected on the operating controls The specimen fast cooling and temperature control works with a refrigeration unit and an electronic control unit The coolant brine flows through the cooling head of the system and cools it While sectioning the pre selected cutting temperature is kept cons
21. angle adjustment Standard knife carrier Disposable blade carrier Magnetic disposable blade carrier Coarse filter Glass fiber reinforced extraction thimble Micro filter Borsilicate micro fiber 99 99 filtration efficiency for 0 1 um particles Option D Max filling amount Max filling amount Frequency Ultrasonic unit ccceccceceeeeeeeeeeeeeeeeceeeeeeeeeceeeeeaeeeeeeeeeaes 1 63 MHz 70 kHz Transportation and storage conditions Storage temperature range 20 C up to 50 C Operating conditions 5 C up to 35 C at a max rel humidity of 60 altitude up to 2000 m M S L for indoor use only Floor loading requirements 300 kg m Power requirements 100 V 50 Hz 12 A 10 100 V 60 Hz 12 A 10 115 V 60 Hz 12 A 10 220 230 V 50 Hz 6 A 10 220 V 60 Hz 6 A 10 240 V 50 Hz 6 A 10 Internal protection Primary circuits Transformer T8AH 100 115 V T4AH 220 240 V Illumination TO 8AH 100 115 V T0 4AH 220 240 V Secondary circuits Window heating Vacutome valve optional All temperatures refer to an ambient temperature of 20 C MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series Cont d on page 3 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 1 2 Pollution degree Overvoltage category Sound pressure Fluids and gases Dimensions Weight HM 550 Series
22. care of the microtome during routine work Caution A regular and or immediate disinfection of the blade carrier the cryo chamber as well as all the other potentially contaminated parts of the instrument is highly recommended All commercially available cryostat disinfection solutions can be used MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 5 4 2 2 2 2 CLEANING THE COOLING LAMELLA Open the cleaning opening via the attached tool fig 135 1 For this insert the tool fig 134 1 through one of the middle ventilation slots Turn the tool by 90 so that the tshaped holder latches into the adjacent slots Pull the tool to open the grid fig 134 1 of the cleaning hole Note The grid of the cleaning hole is kept on the right side by a magnet fig 134 2 an is inserted on the left side Remove the dust from the cooling lamella by means of a commercially available vacuum cleaner Note Carry out this cleaning in regular intervals Thus exending the lifetime of the compressor MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 134 De ae 2 Fig 135 MICRON 5 5 CHANGING THE FLUORESCENT LAMP The fluorescent lamp L8 12 of the cryostat is integrated in the grip fig 136 1 of the heated sliding window Before exchanging the l
23. daily When the daily mode fig 120 has been selected the cylce is carried out daily for seven days max at the preselected defrost time Warning The automatic cycle can only start after the sliding window has been closed MICRON HM 550 Series Ver 2 00 MODE OPTION D MODE WEEKLY WEEKEND YES NO DISINFECT MEDIUM REALTIME DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COARSE FILT RESET RETRACTION ENABLE LANGUAGE Fig 118 Choose UP amp DWN confirm ENTER Fig 119 MODE OPTION D Choose UP amp DWN confirm ENTER Fig 120 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON Mode once a week weekly In case the weekly mode fig 121 has been selected the cycle is carried out at the preselected defrost time on one day per week Warning The automatic cycle starts after the sliding window has been closed Submenu Mode weekly Press the enter button fig 116 10 to select the submenu fig 122 In case the weekly mode has been selected in the submenu Mode Option D the weekday for the cylce must be entered in this submenu Select from Monday until Sunday via the arrow buttons fig 115 5 and 115 6 Confirm with the enter button 116 10 Submenu Weekend Yes No Press the enter button fig 116 10 to select the submenu fig 124 In case the daily cycle has
24. fig 89 1 is located above the right arm rest Press this button to immediately stop the motorized cutting drive When the hand emergency stop is activated the red LED fig 88 5 between the buttons fig 88 3 and 88 4 lights up Note After having activated the hand emergency stop STOP is shown inverted on the display fig 90 After having eliminated the danger and to continue sectioning pull out the red button fig 89 1 The cutting drive can be started again Foot emergency stop The second emergency stop installation is located in the foot pedal fig 91 Migorously step on the foot pedal to immediately stop the motorized cutting drive However the LED fig 88 5 does not light up when the foot emergency stop is pressed Note After having activated the foot emergency stop STOP is shown inverted on the display fig 90 After having eliminated the danger and to continue sectioning release the foot pedal The cutting drive can be started again MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM550 OMV SPECIM CHAMBER COUNTER HM 550 Series SET 25 720 Fig 90 MICRON HM 550 Series 2 19 OPTION V VACUTOME 2 19 1 SETTING THE VACUUM FOR STRETCHING SECTIONS AND DISPOSING OF SECTION WASTE Setting the vacuum To select the suction power or the required vacuum between 0 8 turn the operating knob fig 92 1 0 no suctio
25. medium 118 ml 161425 Section waste tray for instruments without specimen cooling consisting of 1 waste tray 415600 1 intermediate tray 415620 1 brush shelf 415640 Section waste tray for instruments with specimen cooling consisting of 1 waste tray 415610 1 intermediate tray 415630 1 brush shelf 415640 25 coarse filters option V 281070 1 hose with nozzle for Vacutome cleaning option V 570230 1 foot pedal option M 640280 1 instruction manual 387748 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 24 2 ADDITIONAL EQUIPMENT OPTIONAL Specimen chucks 20 mm 30 mm 40 mm 50 x 50 mm 55 x 55 mm 60 x 55 mm 70 x 55 mm Cryo Molds 10 mm 15 mm 22 mm Knife carriers Standard knife carrier Disposable blade carrier EC Magnetic blade carrier Knife carriers for option Vacutome Disposable blade carrier EV Magnetic blade carrier Other accessories Heat extractor Detach block Coarse filter Micro filter Disposable blade SEC 35 50 pcs in dispenser MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf Cat No 715700 715710 715720 715730 715740 715750 715760 570400 570380 570390 705460 705470 705230 705580 705590 524510 281070 624100 152200 HM 550 Series MICRON HM 550 Series 3 THEORY OF OPERATION 3 1 SPECIMEN AND CHAMBER COOLING DEFROSTING The chamber the microtome and the specimen head are cooled by a refrigera
26. that should be sectioned All tools which are necessary to take off sections or b manipulate the specimen must also be cooled as the section will stick to them For this reason the required working accessories should always be stored on the shelves or brush tray in the microtome chamber The heated sliding window should be closed during breaks and while preparing work to avoid warm air getting into the chamber Consequently more favorable work conditions with a lower frost built up on the microtome and knife are achieved In case the instrument is equipped with a Vacutome please note that both the coarse filter and the micro filter are properly inserted in the filter unit When needed replace them and dispose of them according to the respective lab regulations MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 4 2 FREEZING ON THE SPECIMEN The specimen is frozen on to the specimen chuck with clinging groves by means of a freezing medium To freeze on specimens use the fast freezing device or the active deep freezing device on the left side of the chamber When using the fast freezing device put some freezing compound onto the specimen stage which should be outside the chamber at a low positive temperature Then put the specimen onto the stage and surround it with some freezing compound Please note that the frozen tissue remains connected with the freezing medium and the chuck Then insert t
27. through of the cutting window The length of the cutting window is shown in mm within the cutting window symbol on the display Note A cutting window should only be set while the specimen is moved downwards Note In case the cutting window limits are set incorrectly please repeat above mentioned process again MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 83 MICRON 2 18 2 SELECTION OF OPERATING MODES For the motorized cutting movement of the microtome the following operating modes are available continuous stroke single stroke interval stroke 4f the cutting drive is turned off choose one of the above mentioned operating modes by pressing the button MODE fig 85 1 once or twice The selected operating mode is shown on the display as CONTIN SINGLE and INTERV and the corresponding LED in button fig 85 1 lights up Continuous stroke Press button fig 85 1 until the operating mode continuous stroke CONTIN is shown on the display To start a continuous cutting cycle use the foot pedal or press the two start buttons fig 85 3 and 85 4 2770 stop the continuous stroke press foot pedal or one of the two start buttons fig 85 3 or 85 4 However if a cutting cylce has been started it will continue through and stop in the next lower reversal point Single stroke Press button fig 85 1 until the operating mode single str
28. zero device of the specimen clamp i e the specimen clamping together with chuck and specimen latches noticeably into the zero position Afterwards clamp the specimen clamping via the clamping lever The chuck is now in a parallel position with regard to the knife After this orientation now set the cutting suction window Please note that the lower cutting window limit corresponds with the upper edge of the specimen With the motorized coarse feed specimen and knife can roughly be adjusted The process of the first approach between specimen and knife edge can also be carried out via the automatic approach system Then carry out a further gradual feed by using the function trimming Turn the handwheel in a clockwise direction resulting in the first contact between knife and specimen Continue this thus achieving the desired level Any waste should be wiped away in an upward direction with a brush When doing first cuts in combination with a Vacutome these sections are removed automatically While trimming determine the correct force of the vacuum by continued cutting and varying it via the operating knob The setting is optimal when the last section remains stretched on the blade while the penultimate section gets into the suction slot when starting a new cut MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 4 4 SECTIONING AND TAKING OFF SECTIONS For sectioning pre sel
29. 2 7 1 2 7 2 2 7 3 2 7 4 2 7 5 2 7 6 2 8 2 9 2 9 1 2 9 2 2 9 3 2 9 4 2 10 2 10 14 2 10 2 2 10 3 2 10 4 MICROM International GmbH Robert Bosch Sir 49 D 69190 Walldorf cate of Conformity ecautions INTRODUCTION Description of the HM 550 Series Technical data HM 550 Series OPERATING INSTRUCTIONS Setting up the cryostat Initial turn on Basic operational rules Cutting process Cutting movement and retraction Setting section thickness and trimming thickness Chamber cooling Actual and set value of the chamber temperature Function fast freezing for standard instruments Heat extractor optional accessory Feed Specimen coarse feed Speed of the specimen coarse feed Setting the cutting window Automatic approach system Trimming and first cuts Fine feed Turning on off the function retraction Indication of cutting processes Section counter Section thickness sum Remaining travel to front end position Real time Setting the real time WAKE time ACTIVE time and date Setting the real time Setting the WAKE time Setting the ACTIVE time Setting the date MICRON 2 11 2 11 1 2 11 2 2 11 3 2 11 4 2 12 2 12 1 2 12 2 2 13 2 14 2 15 2 16 2 16 1 2 16 2 2 16 3 2 17 2 17 1 2 17 2 2 18 2 18 1 2 18 2 2 18 3 2 18 4 2 18 5 2 18 6 2 19 2 19 1 2 19 2 2 19 3 2 20 2 20 1 2 20 2 2 21 2 22 2 22 1 2 22 2 2 22 3 2 22 4 2 23 2 23 1 2 23 2 2 24 2 24 1 MICR
30. 44 3 5 Fig 45 Fig 46 MICRON 2 10 4 SETTING THE DATE Press the menu button fig 50 9 This way you get into the list of the submenus Via the arrow buttons fig 49 5 and 49 6 select Date This submenu is now shown inverted Via the enter button fig 50 10 confirm this submenu and simultanously open it To choose between day month and year press the reset button fig 50 8 to set the correct date Via the arrow buttons fig 49 5 and 49 6 change the desired field of the date in steps of day month or year Confirm it via the enter button fig 50 10 Note You return immediately to the list of submenus If the setting is not confirmed via the enter button you will quit the submenu automatically after five seconds and you return into the list of submenus The current setting of the submenu is also chosen and will still be valid To quit the submenu press the menu button fig 50 9 Now continue working with the selected settings MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Vers 1 00 DEFROST TIME WAKE TIME STANDBY DELAY REAL TIME DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COARSE FILT RESET RETRACTION ENABLE LANGUAGE ILLUMINATION Fig 47 Choose UP amp DWN Confirm ENTER Fig 48 5 8 Fig 49 Fig 50 MICRON 2 11 DEFROSTING 2 11 1 SETTI
31. MICRON MICROTOME CRYOSTAT HM 550 Series Options OMVPD INSTRUCTION MANUAL MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON HM 550 Series CERTIFICATION MICROM International GmbH certifies that this instrument has been tested and checked carefully Its technical data was verified before shipment to be in accordance with the published specifications The instrument complies with applicable international safety regulations WARRANTY This MICROM product is warranted against defects in material and workmanship for a period of 1 year Parts which prove to be defective during the warranty period will be repaired or replaced free of charge by MICROM International GmbH No other warranty is expressed or implied Unauthorized modification or repair by third party persons will void the warranty The warranty will expire in case of improper or wrong use of the instrument and in case the warning and precautionary messages are not observed MICROM International GmbH is not liable for any occurring damage Errors and ommissions excepted Subject to amendment and improvement without further notice This instruction manual will be supplied together with each instrument Further copies can be ordered at the nearest MICROM sales office by giving the serial number of the instrument the number of the instruction manual and the date d issue This instruction manual is available in the followi
32. NE FILTER RESET COARSE FILT RESET RETRACTION ENABLE LANGUAGE ILLUMINATION Fig 39 WAKE TIME Choose UP amp DWN Confirm ENTER Fig 40 5 Fig 41 Fig 42 MICRON 2 10 3 SETTING THE ACTIVE TIME Press the menu button fig 46 9 This way you get into the list of the submenus Via the arrow buttons fig 45 5 and 45 6 select Active Time This submenu is now shown inverted Via the enter button fig 46 10 confirm this submenu and simultanously open it To change the ACTIVE time at intervals of 1 hour steps 1 to 9 h press the arrow buttons fig 45 5 or 45 6 Confirm it via the enter button fig 46 10 Note You return immediately to the list of submenus If the setting is not confirmed via the enter button you will quit the submenu automatically after five seconds and you return into the list of submenus The current setting of the submenu is also chosen and will still be valid To quit the submenu press the menu button fig 46 9 Now continue working with the selected settings MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Vers 1 00 DEFROST TIME WAKE TIME STANDBY DELAY REAL TIME DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COARSE FILT RESET RETRACTION ENABLE LANGUAGE ILLUMINATION Fig 43 AKTIV TIME H Choose UP amp DWN Confirm ENTER Fig
33. NG THE DEFROSTING TIME Press the menu button fig 54 9 This way you get into the list of submenus Mia the arrow buttons fig 53 5 and 53 6 select Defrost Time This submenu is now shown inverted Mia the enter button fig 54 10 confirm this submenu and simultaneously open it 2710 change the instrument s defrosting time at intervals of 15 minutes press the arrow buttons fig 53 5 or 53 6 Confirm with enter fig 54 10 Note You return immediately to the list of submenus lf the setting is not confirmed via the enter button you will quit the submenu automatically after five seconds and you return into the list of submenus The current setting of the submenu is also chosen and will still be valid To quit the submenu press the menu button fig 54 9 Now continue working with the selected settings Note Defrosting can only be carried out at the set defrosting time if the real time is set correctly see part 2 10 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Vers 1 00 DEFROST TIME WAKE TIME STANDBY DELAY REAL TIME DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COARSE FILT RESET RETRACTION ENABLE LANGUAGE ILLUMINATION Fig 51 DEFR TIME Choose UP amp DWN Confirm ENTER Fig 52 3 Fig 53 Fig 54 MICRON 2 11 2 DEFROSTING CYCLE Every 24 hours the evaporat
34. OM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Defrosting Setting the defrost time Defrosting cycle Interrupting a defrosting cycle Emptying the defrosting liquid Customer specific settings Setting the contrast Selecting the language Illumination of the cooling chamber Service settings Specimen orientation Knife carriers Standard knife carrier Disposable blade carrier EC Magnetic disposable blade carrier M Option O specimen cooling Actual and set value of the specimen temperature Specimen orientation with zero device with option O Option M motorized cutting drive Setting the cutting window Selecting the operating modes Setting the cutting speed Start and stop of the cutting drive Handwheel brake Emergency stop Option V Vacutome Setting the vacuum for stretching sections and disposing of section waste Inserting and replacing the filter unit Setting the suction window Knife carrier for Vacutome Disposable blade carrier EV Magnetic disposable blade carrier MV Option P active deep freezing device with Peltier element Option D Integrated automatic unit for the application of disinfection media Preparing and starting the option D Interrupting the option D Programming the option D Cleaning after a cycle with the option D Error code indication Definition of the error codes Error codes of the option D Accessories Standard equipment MICRON HM 550 Series
35. ROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON Note Only special MICROM magnetic blades must be used Inserting the blade Insert the blade fig 103 1 into the blade slider fig 103 2 and put it on the magnetic surface fig 103 2 of the blade carrier from the side Note To move the blade to the side press the blade slider downwards so that the blade is properly put against the ledge fig 103 3 of the blade carrier To move the blade to the side always use the blade slider fig 104 2 and shift it If the cutting area of the blade is not longer usable the blade can be moved Put the blade slider fig 104 2 onto the blade and shift it Note The blade slider must always be put on in a way that the inscription MICROM can be read This way it is guaranteed that the slanted edge fig 104 5 of the blade slider shows to the rear side of the blade Caution To avoid the danger of injury on the blade during adjustment of specimen always position the knife guard fig 103 10 over the blade edge MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON Clearance angle adjustment Loosen the clamping lever fig 103 7 Swivel the upper part of the magnetic blade carrier on the base until the desired clearance angle is adjusted The set clearance angle can be read on the scale fig 103 8 on the left
36. SE FILT RESET RETRACTION ENABLE LANGUAGE ILLUMINATION Fig 66 LANGUAGE ENGLISH Waehlen UP amp DWN Bestaetigen ENTER Fig 67 Fig 68 Fig 69 MICRON HM 550 Series MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 13 ILLUMINATION OF THE COOLING CHAMBER To illuminate the cooling chamber a fluorescent lamp is located in the handle of the sliding window The fluorescent lamp is automatically turned on when the instrument is turned on 27he lamp remains on as long as the instrument is in its active status see standby status part 2 3 Used lamps can be changed by the user himself see part 5 4 Turning the light on off Press the menu button fig 72 9 Mia the arrow buttons fig 72 5 and 72 6 select Illumination Confirm it via the enter button fig 72 10 Mia the arrow buttons fig 71 5 and 71 6 select either ON or OFF Tonfirm it via the enter button fig 72 10 Press the menu button fig 72 9 to quit the menu MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Vers 1 00 DEFROST TIME WAKE TIME STANDBY DELAY REAL TIME DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COARSE FILT RESET RETRACTION ENABLE LANGUAGE ILLUMINATION Fig 70 Fig 71 Fig 72 MICRON HM 550 Series 2 14 SERVICE SETTINGS Vers 1 00 WAKE TIME Note Ree
37. TIER chuck choose the function fast freezing If this function is activated in due time approx 2 3 min before freezing on the fast freezing plate Choose UP amp DWN Confirm ENTER can achieve a temperature of 60 C Note This fast freezing device can only be Fig 106 activated at a chamber temperature of less than 10 C Note The upper side of the freezing station must not be covered by frost ice To avoid this use acetone ethanol or the like Press the menu button fig 108 9 Via the arrow buttons fig 107 5 and 107 6 select Peltier Station Confirm it via the enter button fig 108 10 Via the arrow buttons fig 107 5 and 107 6 select HEAT COOL or OFF Confirm the selected function via the enter button fig 108 10 The Peltier functions are turned off automatically after 8 min To active or deactivate the fast freezing function press button fgi 107 2 for 1 sec fig 109 Fig 107 Vers 1 00 DEFROST TIME WAKE TIME STANDBY DELAY REAL TIME DATE REQUEST DEFROST HM550 OMVP SET INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COARSE FILT RESET RETRACTION ENABLE LANGUAGE JLLUMINATION SPECIM 725 CHAMBER 20 FREEZE ACTIVE COUNTER O Fig 105 FINE 3 Fig 109 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series 2 If HEAT is selected the Peltier elemen
38. actual temperatures electronic control with user oriented touchpad keyboard battery buffered memory and self diagnostic system Standby and sleep status for noise reduction and energy savings Automatic defrosting and in addition a manual defrost cycle which can be activated when needed Defrost interrupt on keystroke request Integrated fast freezing device with controlled cooling element down to 60 C option P Sliding window with heater and integrated fluorescent lamp for cryo chamber Brush shelf and storage space inside the cryo chamber Large flat storage space on top of the housing with integrated storage space for marked slides Rotary Microtome with backlash and maintenance free cross roller bearings in stainless steel Electro mechanical feed system Section thickness setting from 1 to 100 microns up to 10 um in 1 um increments up to 20 um in 2 um increments up to 70 um in 5 um increments and up to 100 um in 10 um increments Trimming thickness setting from 5 to 500 microns up to 10 um in 5 um increments up to 100 um in 10 um increments up to 200 um in 20 um increments and up to 500 um in 50 um increments Specimen retraction during the return stroke with optical indication Horizontal feed range 28 mm Vertical cutting stroke 60 mm Max specimen size 75 x 55 mm Motorized coarse feed in two directions with three speed selections Limit indication and automated switch off at front and rear limits of ho
39. amp turn off the mains switch and unplug the instrument Open the sliding window halfway Turn the two red knurled screws fig 136 2 which are placed on the right and left lower side of the sliding window in a counter clockwise direction and remove them Remove the grip fig 136 1 from the upper side of the sliding window Turn the lamp by 90 forwards or backwards and remove it from the sockets fig 136 4 Insert the new lamp from above into the socket and turn it by 90 Note Only use the lamp type L8 12 cat no 346190 which was installed in the factory Note Whenever exchanging the fluorescent lamp please also exchange the starter S10 Philips cat no 322710 2 2 2 Turn the starter fig 136 5 in a counter clockwise direction and remove it from the socket Insert the new starter into the socket Turn the new starter clockwise until the starter is positively locked Place the grip fig 136 1 onto the illumination ledge from above Screw in the two red knurled screw clockwise from below and tighten them Plug in and then turn on the instrument and check the illumination MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 136 MICRON HM 550 Series PART 6 CONDITIONS FOR THE TRANSPORTATION OF THE INSTRUMENT 6 1 TAKING BACK THE INSTRUMENT FOR REPAIR OR ROUTINE MAINTENANCE Repair or maintenance work are normally ca
40. ards forwards MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series 5 2 REMOVING THE MICROTOME In the left rear part of the microtome there is the so called connector housing including the two electrical connections and for the option O specimen cooling two cool technical quick couplings as well Pull off the red and blue coded electrical connector For this touch the connectors on their connector housings Insert the blue coded connector fig 130 1 into the park position 9 fig 130 9 of the fast freezing station Insert the red coded connector fig 130 2 into the park position 10 fig 130 10 Warning Cover the connector sockets via the attached caps as cooling liquid is critical for the electrical connectors Note Put a small cloth under the quick coupling of the option O before pulling it off as a small amount of cooling liquid might leak out before disconnecting I S a Read user manual For the option O pull off the quick coupling For this touch the connectors on the ring sleeve and pull them towards the operator to the front and pull the connector off Insert the left connector into park position 11 fig 130 11 and the right one into park position 12 fig 130 12 of the fast freezing Fig 130 station If the positions are mixed up this will have no influence on the function Note The described electrical cool technical
41. ation M icrotome Cryostat Type reference HM 550 O M V P The designated product complies with the laid down regulation DIRECTIVE 98 79 EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 27 October 1998 on in vitro diagnostic medical devices The designated product complies with the EC regulations by strictly observing the following norms DIN EN ISO 14971 2001 03 Medical devices Application of risk management to medical devices ISO 14971 2000 DIN EN 61010 1 2002 08 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements IEC 61010 1 2001 IEC 61010 2 101 2003 09 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 101 Particular requirements for In Vitro Diagnostic IVD M edical instruments DIN EN 61010 2 081 2002 12 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 081 Particular requirements for automatic and semi automatic laboratory equipment for analysis and other purposes IEC 61010 2 081 2001 DIN EN 61326 2002 03 Electrical equipment for measurement control and laboratory use EMC requirements IEC 61326 1 1997 A1 1998 A2 2000 German version EN 61326 1997 A1 1998 A2 2001 DIN EN ISO 9001 2000 Quality management systems Requirements ISO 9001 2000 Hans Heid Walldorf 05 December 2003 Managing Director MICROM International GmbH Robert Bo
42. been selected in the submenu Mode Option D you should enter here if a cycle should be carried out during the weekend or not Select between YES and NO via the arrow buttons fig 115 5 and 115 6 Confirm with the enter button 116 10 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MODE OPTION D Choose UP amp DWN confirm ENTER Fig 121 pereon Ver 2 00 DEFROST TIME WAKE TIME STANDBY DELAY OPTION D ON OFF MODE OPTION D MODE WEEKLY WEEKEND YES NO DISINFECT MEDIUM REALTIME E REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST Fig 122 MODE WEEKLY Choose UP amp DWN confirm ENTER MENU Ver 2 00 PELTIER STATION DEFROST TIME WAKE TIME STANDBY DELAY OPTION D ON OFF MODE OPTION D MODE WEEKLY WEEKEND YES NO DISINFECT MEDIUM REAL TIME DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST WEEKEND YES NO Choose UP amp DWN confirm ENTER Fig 125 MICRON HM 550 Series Submenu Expiry date of the disinfection Ver 2 00 medium DEFROST TIME aa WAKE TIME 2 Press the enter button fig 116 10 to select the submenu Disinfect Medium Enter the date the expiry date into the six STANDBY DELAY OPTION D ON OFF MODE OPTION D MODE WEEKLY WEEKEND YES NO DISINFECT MEDIUM REAL TIME l E REQUEST DEFROST INTERRUPT DEFROST digit date format day month year via the arrow buttons
43. belt tension Defective lamp Defective starter HM 550 Series Clean with a dry cloth or brush Replace anti roll plate Move the knife Select a higher temperature Remove the cause Select a deeper temperature Trim the specimen in a parallel way select a thicker section thickness Readjust the anti roll plate Align parallel Readjust the clearance angle Move the knife Readjust the anti roll plate Readjust the anti roll plate Move the knife Readjust Check clamping Check clamping Remove and clean Please call a service technician Check replace Check replace MICRON HM 550 Series Automatic approach system Temperature outside the area ACA cannot be started Specimen clamping outside the Check cutting window Contact between specimen and Check blade knife already at the ACA start Automatic approach system does Specimen too cold not stop Note In case of malfunctions and or service work please turn off the instrument and contact your local dealer MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series 5 MAINTENANCE AND CARE OF THE CRYOSTAT 5 1 SHUTTING OFF FOR CLEANING Cleaning care and decontamination of the cryostat depends on how frequently the instrument is used However it is recommended to shut the instrument off every 6 8 weeks Caution As the following work might be hazardous because of the danger of
44. brake 7 Brake LED 8 Button to loosen the handwheel brake MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series mM Fig 4 MICRON HM 550 Series 2 OPERATING INSTRUCTIONS 2 1 SETTING UP THE CRYOSTAT Unpacking the instrument Detach the three packing straps and remove them Remove the screws on the which are upwards folded Remove the upper wooden cover fig 5 1 from the packing Remove the screws from the packing Lift the packing fig 5 2 over the instrument Remove the upper and lower supporting foams fig 5 3 5 4 5 5 5 6 from the cryostat The hinges of the upper wooden cover have central holes These holes will now be put onto the vertical screws of the bottom plate fig 5a 7 Warning The screws which are to be used are marked with arrows Note The upper wooden cover now serves as an incline fig 5a 1 on which the cryostat can be moved from the palett to the floor The accessories of the cryostat are placed on the foot rest of the cryostat packed in a separate carton The tool fig 56 2 for the height adjustment of the rollers is packed in this carton by means of this tool until the cryostat can be rolled from the palett Note The handle on the front side of the cryostat is used to move the instrument from the palett Caution The weight of the cryostat is very high While the instruments rolls down on the incline it
45. bstances as solvents Therefore water based disinfection media must be used with the following physical characteristics surface tension larger than 65 x 10 N m water 72 x 10 N m viscosity smaller than 1 3 x 10 kg ms water 1 x 10 kg ms pH value is 7 Note Please be advised that with regard to the above mentioned characteristics the selected medium is to be fumigated It is the responsibility of the user to select a suitable disinfection medium with regard to the above mentioned safety precautions and thus the related disinfection results Note We would like to emphasize that the usage of the device does not release the user from noticing and observing the operational regulations MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Ver 2 00 MODE OPTION D MODE WEEKLY WEEKEND YES NO DISINFECT MEDIUM REAL TIME DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COARSE FILT RESET RETRACTION ENABLE LANGUAGE Fig 112 MICRON Starting the first phase of the cycle is determined by the programmed defrost time Each cycle finishes with a defrosting of the cooling system Afterwards the chamber and specimen temperatures which have been selected before are set automatically again Note It is not possible to operate the cryostat during a all phases We recommend to program an overnight run of the cycle MICROM In
46. chamber illumination goes off and the display switches to a large indication of the chamber actual temperature This is the clear sign for the standby status and gives the operator information on the chamber temperature also from a greater distance When the instrument is not operated for another hour the cryostat switches to the sleep status The display illumination goes off However the equipment on indicator fig 2 1 then lights up and shows that the instrument is still on Note While in the sleep status the temperature control of the chamber temperature features a reduced control accuracy The specimen temperature in the sleep status is always kept at 15 C The set temperature of the chamber is kept on a temperature of 20 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series SPECIM 725 CHAMBER 20 COUNTER O FINE 3 Fig 9 Fig 10 MICRON To return to the active operating state press any key on the control panels or move the handwheel Also when reaching the WAKE time see part 210 2 the instrument is in the active state again UP DOWN buttons These two buttons fig 11 5 and 11 6 are used to change pre selected values of different kinds in connection with the chosen function Section thickness trimming thickness pecimen temperature thamber temperature time settings Note To operate the instrument easily and comfortably with a few b
47. d be advantageous This can easily be done by means of the orienting specimen holder on the microtome Before the chuck can be inserted pull off the spring loaded eccentric bolt fig 81 1 Insert the chuck while the bolt is pulled off Afterwards insert the eccentric bolt fig 81 1 again For the specimen orientation now loosen the clamping lever fig 81 2 towards the front The entire specimen head can now be oriented manually together with specimen cooling if equipped in all directions by approx 8 After having achieved the desired alignment press the clamping lever fig 81 2 upwards to fix this position For the further orientation of the specimen the chuck can be rotated by 360 Turn the spring loaded eccentric lever fig 81 1 either to the right or left side Then the chuck is loosened Turn the chuck together with the specimen as desired Then bring the eccentric lever fig 81 1 again into clamping position by turning it to right or left side Note The specimen orientation includes a zero device for the specimen chuck is always parallel in relation to the knife Zero position can be felt noticeably MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 81 MICRON 2 18 OPTION M MOTORIZED CUTTING DRIVE Sectioning can be carried out either manually by turning the handwheel or by means of a motorized cutting drive
48. d section or trimming thickness via button up fig 16 5 or down fig 16 6 2 he altered values are shown on the display Fig 15 FINE 3 The graduation of the section thicknesses which can be pre selected is divided into 5 ranges range graduation from 1 um to 10 um 1 um from 10 um to 20 um 2 um from 20 um to 70 um 5 um from 70 um to 100 um 10 um The graduation of the trimming thicknesses which can be pre selected is divided into 4 ranges range graduation Fig 16 from 5 um to 10 um 5 um from 10 um to 100 um 10 um from 100 um to 200 um 20 um from 200 um to 500 um 50 um MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 6 CHAMBER COOLING 2 6 1 ACTUAL AND SET VALUE OF THE CHAMBER TEMPERATURE The actual and set values of the chamber cooling are shown in C on the display fig 17 On the right side of the display the actual value is also shown graphically Press button fig 18 3 for chamber temperature 7CHAMBER is then shown inverted The set value settings are made via the up button fig 18 5 Mown button fig 18 6 Note The valid range of the set value goes from 10 C down to 35 C The actual value of the chamber temperature is shown as actual temperature and as a graphic After having chosen the requested values the display automatically returns to its basic indications after three seconds i e fine section thickness is
49. d specimen chucks should be used only The specimen clamping of the microtome is connected with a sensitive electronic detecting device The temperature sensor on the left side of the microtome housing is also connected with such a device Malfunctions might occur after electrostatic discharges on one of the above mentioned parts Recommendation Before touching the cylinder head or temperature sensor touch another metallic part e g knife carrier inner wall of chamber or waste tray to guarantee a safe discharge When working with the active deep freezing device sufficient time must have been passed to allow this device reaching a temperature of 60 C For optimal sectioning the knife and anti roll guide must be cleaned of paraffin waste Only use a sharp knife and exactly adjust the anti roll guide in relation to the knife edge WARNING During defrosting remove the specimens from the cooling chamber as the temperature inside the chamber will increase Do not leave or store tissue inside the cryostat over a longer period Due to a power failure or other unexpected malfunctions of the instrument the specimen might be damaged How to avoid malfunctions when working with the Vacutome Exactly adjust the cutting and suction window to the specimen size to avoid inadvertent disposal of good sections Set the suction vacuum according to the specimen and the selected section thickness MICROM Internatio
50. de must be press downwards so that the blade is put properly against ledge Turn the lever fig 77 2 to move the anti roll plate fig 77 1 If the cutting area of the blade is no longer usable the blade can be moved Put the blade slider fig 79 1 onto the blade and shift it Note The blade slider must always be put on in a way that the inscription MICROM can be read This way it is guaranteed that the slanted edge of the blade slider shows to the rear side of the blade Caution To avoid the danger of injury on the blade during adjustment of specimen always position the knife guard over the blade edge Fine adjustment of the anti roll device The fine adjustment of the anti roll device is carried out via the knurled screw fig 77 3 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON HM 550 Series Setting the clearance angle Loosen the clamping lever as well as the clamping screw on the right side of the magnetic disposable blade carrier Swivel the upper part of the blade carrier on the base until the desired clearance angle is achieved The clearance angle can be read on the scale on the left side of the base Again tighten the clamping lever and the clamping screw The selected clearance angle is thus fixed in its position Note Usable cuts are only achieved with a clearance angle between 24 and 30 MICROM Inte
51. e temperature sensors are faulty when the end position of the specimen clamping has not been reached when there is a high pressure in the cooling system and when the function of the automatic approach system is faulty see part 2 7 4 Note If an error code appears on the display of your instrument please note it down and contact your sales organisation where you bought the instrument or directly contact MICROM International GmbH 2 23 1 DEFINITION OF THE ERROR CODES ERROR OF A TEMPERATURE SENSOR Four different error codes which indicate a defect on a temperature sensor can appear on the display The message is shown on the second line from below on the display fig 127 1 SPECIMEN SENSOR ERR The instrument recognizes a faulty operation on the specimen temperature sensor which is placed in the specimen head 2 BOX SENSOR ERR The instrument recognizes a faulty operation on the box sensor which is placed on the base plate of the microtome 3 EVAP SENSOR ERR The instrument recognizes a faulty operation of the defrost limiting sensor which is placed between the fins of the evaporator MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf kkk SPECIM CHAMBER COUNTER FEIN 3 225 720 0 HM 550 Series az 21 Zz CONTIN TRIM 20 SPECIMEN SENSOR ERR ER 5 R Fig 127 MICRON Note However you can work with the instrume
52. e blades first loosen the lever fig 101 2 and remove the spacer strip The blade is clamped via the clamping plate which is placed on the rear part of the blade carrier To insert the blade now loosen the clamping lever fig 101 2 Note Press the clamping plate on its lower area to open the slot behind the clamping plate Push the blade from the left side into the slot behind the clamping plate fig 101 1 as far as possible Close the clamping lever fig 101 2 Thus fixing the blade in its position If the cutting area of the knife is no longer usable the knife can be moved Tilt the knife guard fig 101 10 forwards Loosen the clamping lever fig 101 2 The blade is no longer clamped and can be moved e g by means of a brush until a suitable position of the blade is found Clamp again the clamping lever fig 101 2 Caution To avoid the danger of injury on the blade during adjustment of specimen always position the knife guards fig 101 10 over the blade edge MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON Setting the clearance angle Loosen the clamping lever fig 101 7 Swivel the upper part of the blade carrier on the base until the desired clearance angle is achieved The clearance angle can be read on the scale fig 101 8 on the left side of the base Again tighten the clamping lever fig 101 7
53. e of the knife or the specimen is outside the temperature range of 5 C and 35 C C which is valid for the automatic approach function only MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON Press button fig 25 2 The error message goes off Note In this case the function automatic approach cannot work The approach between specimen and knife edge must be carried out via the coarse feed button fig 25 3 see part 2 7 1 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 2 7 5 TRIMMING AND FIRST CUTS After the specimen and the knife are adjusted further gradual feed for trimming can be carried out using the function trimming For different sectioning series deeper layers of the specimen can be reached with the function trimming Make sure that the trimming thickness setting is selected via the button fig 26 4 Trimming thickness is shown inverted on the display Press the arrow buttons fig 26 5 or 26 6 to select the desired trimming value 7 urn the handwheel in a clockwise direction to carry out the trimming feed in the upper reversal point of the cutting movement Note If the trimming function is carried out via the motorized drive please read part 2 18 option M 2 7 6 FINE FEED After having adjusted knife and specimen as well as having trimmed the specimen sectioning can be started
54. ear end position of the specimen clamping is reached the coarse feed turns off 7he LED in button fig 21 4 blinks which means that the specimen clamping is in its rear end position Feed travel of the specimen clamping Press button fig 21 5 Briefly press this button to carry out a forward travel of that trimming section thickness which was chosen via the control panel and shown on the display The continuous forward travel is only carried out as long as the button fig 21 3 is being pressed 2 Warning With the function coarse feed forwards specimen and knife are adjusted very quickly To avoid damage to specimen and knife the following points are of utmost importance MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON Take note that the specimen and knife edge do not come in contact with each other Varefully observe the narrowing gap between specimen and knife edge to stop the function coarse feed forwards in time before the specimen touches the knife When the instrument is just carrying out the retraction movement and the function coarse feed forwards is selected the coarse feed movement is carried out and the retraction is annulled When the front end position of the specimen clamping is reached the coarse feed turns off 7he LED in button fig 21 3 lights up and shows that the specimen clamping has reached its front end position MICROM International GmbH
55. ect the desired section thickness by means of the buttons on the operating controls Put the anti roll plate against the knife by means of the knob Turn the handwheel in a clockwise direction or use the motorized cutting drive to carry out sectioning The section slides into the space between the knife and the anti roll guide Then remove the anti roll guide by means of the knob The section sticks to the knife surface Transfer the specimen now onto a slide by using a brush or tweezers For instruments which are equipped with a Vacutome also pre select the desired section thickness by means of the buttons on the operating controls see part 2 4 In addition set the desired vacuum on the operating knob Put the vacuum anti roll hood against the blade by means of the lever Turn the handwheel in a clockwise direction or use the motorized cutting drive to carry out sectioning At the same time the vacuum prevailing in the slot between vacuum anti roll hood and blade draws the section over the blade The section remains stretched on the blade Open the vacuum anti roll hood to pick up the section onto a slide MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 4 5 HOW TO AVOID MALFUNCTIONS To cut usable sections the following points are of utmost importance Condition of the knife edge probably move it horizontally to the left or right side Check adjustment of anti rol
56. ected settings Fig 65 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 12 2 SELECTING THE LANGUAGE The information on the display can be shown in four different languages The following languages are available German English French Spanish To select or change one of these languages press the menu button fig 69 9 This way you get into the list of submenus Mia the arrow buttons fig 68 5 and 68 6 select the submenu language This submenu is now shown inverted Mia the enter button fig 69 10 confirm this submenu and simultaneously open it Mia the arrow buttons fig 68 5 and 68 6 select the desired language English Deutsch Espagnol Fran ais Mia the q ter button fig 69 10 confirm the setting Note You return immediately to the list of submenus If the setting is not confirmed via the enter button you will quit the submenu automatically after five seconds and you return into the list of submenus The current setting of the submenu is also chosen and will still be valid To quit the submenu press the menu button fig 69 9 Now continue working with the selected settings MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Vers 1 00 DEFROST TIME WAKE TIME STANDBY DELAY REAL TIME DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COAR
57. fection medium first loosen the rapid action hose fig 137 Then remove the container from the supports Clean and disinfect the cryo chamber according to the respective applicable lab regulations Unscrew the handwheel handle for transportation Before the transportation loosen the fastening screws of the rollers Keep the heated sliding window closed during transportation Note If the new site of installation can be reached in less than half an hour keep the sliding window dosed so that the cryo chamber does not heat up This avoids the formation of condensation water inside the chamber Note fig 137 It is recommended that at least two persons are available for the transportation Measures for turning on again Again install the blade carrier section waste trays and the brush shelf Attach the handwheel handle again 2 After having turned on the instrument and after having reached the set temperature the instrument is ready of operation again Note Blade carrier which was heated up to ambient temperature needs approx 1 h in the chamber at 25 C to be able to section specimens again MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON For transportation outside closed buildings please observe the following measures Turn off the instrument Unplug the unit Remove blade and or knife from blade and or knife carrier Remove
58. fig 115 5 and 115 6 Press the reset button fig 116 8 to change between day month and year COARSE FEED SPEED Confirm with the enter button fig 116 10 i In case the message Desinf Medium is Fig 126 shown on the display confirm and delete this message via the enter button fig 116 10 Note After having pressed the enter button this message does not re appear on the display Note The expiry date which is given from the manufacturer of the disinfection medium mainly refers to unopened containers 2 22 4 CLEANING AFTER A CYCLE WITH OPTION D Chemicals Working areas which are accessible for the user and get into contact with the disinfection solution show remainders of the disinfection medium after some cycles Depending on the disinfection medium please wear safety clothing e g protective gloves Wipe off these parts with e g 70 ethanol Also empty the container for the defrosting liquid from time to time as a part of the disinfection liquid is collected here The liquids must be disposed of according to your respective lab regulations MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 23 ERROR CODE INDICATION The instrument has an error code indication to define faster and better possible malfunctions The error codes describe so called system errors Additionally error messages are shown on the display as text messages e g when th
59. g the function detach Another possibility to detach the specimen is to warm it up outside the chamber The specimen temperature control is supplied via a secondary cooling circuit The coolant flows through the cooling head serving as an evaporator of the system and cools it in combination with a Peltier element MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON A thermo electrical element is coupled with the evaporator housing on the specimen head Via a set value input for the specimen head on the control panel the electronics control the thermo electrical element at any desired temperature between 10 C and 50 C Inevitably when working on the microtome the dry cold air of the microtome chamber mixes with the warm humid air outside the chamber Frost forms on the finned evaporator The increasing thickness of the frost reduces the efficiency of the evaporator For this reason the daily defrosting of the evaporator becomes necessary To carry out the defrosting cycle a heating warms the evaporator from below Set the time of the daily defrosting on the control panel The period of the defrosting takes approx 40 min and varies according to the thickness of the frost Possible frost built up on the microtome or on the knife carrier are not removed by defrosting the instrument However the frost recedes after defrosting as now the evaporator is fully efficient again In addition the instrume
60. ge with your fingers The cutting edge is extremely sharp WARNING OF UNHEALTHY OR IRRITATING SUBSTANCES During the intended use of the option D smallest amounts of irritating aerosols which are based ont quaternary compounds might occur For this reason please care for sufficient ventilation To avoid negative effects on your health while in direct contact with the disinfection medium or its residues always wear respective safety clothing e g protective glasses laboratory gloves MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series WASTE DISPOSAL All debris waste defrosting liquid as well as infectious and radioactive contaminated material from operation must be disposed of in accordance with the respective regulations of the lab Disinfection and cleaning liquids as well as section waste must be disposed of according to the respective regulations for special waste MICROM International GmbH Robert Bosch Sir 49 D 69190 Walldorf MICRON HM 550 S eries MICROM International GmbH Robert Bosch Sir 49 D 69190 Walldorf MICRON 1 INTRODUCTION 1 1 DESCRIPTION OF THE HM 550 SERIES Open top cryostat Modular design Stainless steel cooling chamber Chamber temperature regulation variable from 10 C down to 35 C When the instrument is equipped with the option O the specimen temperature can be controlled from 10 C down to 50 C Graphic LC display of set and
61. h of the cutting window is shown in mm within the cutting window symbol on the display 270 activate the automatic cutting window again press button fig 23 8 and while this button fig 23 8 is still being pressed also press the button fig 25 1 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON Note A cutting window should only be set while the specimen is moved downwards If by mistake a cutting window limit is set during return travel of the specimen the set limits are applied to the cutting movement accordingly Note In case the cutting window limits are set incorrectly please repeat above mentioned process again Note If no cutting window is needed briefly press the button fig 25 1 twice The automatic approach system can now be released in any position MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 2 7 4 AUTOMATIC APPROACH SYSTEM The automatic approach system performs the fast and exact approach of the specimen towards the knife edge and can be applied between 5 C and 35 C with the MICROM freezing medium Note Use automatic approach system with conventional freezing medium only down to 35 C chamber and or specimen temperature Caution For your personal safety before activating the automatic approach system move the anti roll plate onto the blade against possible splin
62. h the selected settings The letters REN in the status line on the display go off if the retraction function has been turned off Note The letter R however might still be on This function goes off only after having passed from the return travel again to the cutting movements via the handwheel MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Vers 1 00 DEFROST TIME WAKE TIME STANDBY DELAY REAL TIME DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COARSE FILT RESET RETRACTION ENABLE LANGUAGE ILLUMINATION Fig 27 5 Fig 28 10 Fig 29 MICRON 2 9 INDICATION OF CUTTING PROCESSES Further information on the cutting processes can be seen on the display Press the scroll enter button fig 30 10 to show the various functions one after the other Note Within the menu the scroll enter button is used to confirm submenus and or settings During operation i e outside the menu this button is used as a scroll button The following information on the carried out cutting processes of the instrument can be read on the info line of the display humber of sections um of section thicknesses Temaining travel to the front end position teal time Press the scroll button fig 30 10 until the required information lights up on the display 24f no information is required in this line of the di
63. he chamber rises and thus the tissue specimen would be damaged MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Vers 1 00 DEFROST TIME WAKE TIME STANDBY DELAY REAL TIME DATE REQUEST DEFROST INTERRUPT DEFROST COARSE FEED SPEED CONTRAST FINE FILTER RESET COARSE FILT RESET RETRACTION ENABLE LANGUAGE ILLUMINATION Fig 55 REQUEST DEFROST Choose UP amp DWN Confirm ENTER Fig 56 5o B Fig 57 Fig 58 MICRON HM 550 Series MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 11 3 INTERRUPTING A DEFROSTING CYCLE If needed the daily defrosting can be interrupted or cancelled For this press the menu button fig 60 9 This way you get into the list of submenus Via the arrow buttons fig 59 5 and 59 6 select the submenu request defrost This submenu is now shown inverted Via the enter button fig 60 10 confirm this setting and simultaneously open this submenu Via the arrow buttons fig 59 5 or 59 6 select ON or OFF ON is selected when defrosting is to be interrupted Via the enter button fig 60 10 confirm the setting Note You return immediately to the list of submenus lf the setting is not confirmed via the enter button you will quit the submenu automatically after five seconds and you return into the list of submenus The current setting of the submenu is also chosen and wi
64. he enclosed foot pedal must be connected to the socket fig 7 3 only Loosen the transportation screw on the handwheel which solidly fixes the handwheel on the instrument Then install the attached handwheel handle on the handwheel 24f equipped with the option V check that the micro filter is inserted in its proper position dnsert coarse filter into the filter unit Caution Section waste is collected in the coarse filter It must be replaced at least once a week depending on the amount of debris and nature of the specimens The coarse filter is to be understood as a disposable filter and must not be used again The micro filter must be replaced at regular intervals at least semi annually Section waste as well as coarse and micro filters are to be disposed of according to the respective lab regulations For this please see Inserting and replacing the filter unit part 2 19 2 Note The serial interface figl 7 4 must only be used for service purposes and must not be used by the user for its intended use Only MICROM specific programming instruments must be connected to this interface MICROM International GmbH Robert Bosch Sir 49 D 69190 Walldorf HM 550 Series MICRON HM 550 Series 1 Power switch 2 Mains plug 3 Socket for foot pedal 4 Serial interface MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 2 INITIAL TURN ON Note The kind
65. he specimen stage into one of the fast freezing stations The standard version is equipped with a fast freezing station with 12 stations A cryostat with option P has an active deep freezing device with eight freezing stations and another four stations with Peltier element Start the function Fast freezing of standard instruments in due time before freezing on the specimen It will take the deep freezing device approx 2 3 min to achieve a temperature of max 60 C If the instrument is equipped with a heat extractor additional equipment the specimen can be frozen faster and more evenly Put the pre cooled heat extractor onto the specimen from above This way the specimen is frozen at the same time from above and below which reduces the formation of artefacts considerably MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 4 3 ORIENTATION AND TRIMMING Clamp the specimen chuck together with the frozen specimen into the specimen holder For this loosen the orienting lever standard version and or spring loaded eccentric lever version with option O and insert the chuck into the specimen clamping To loosen the chuck turn the eccentric lever Loosen the clamping lever to have the possibility to adjust the specimen parallel in relation to the cutting edge Afterwards press the clamping lever to the rear To position the chuck parallel in relation to the knife please use the
66. heck knife clamping Check clamping Again freeze on the specimen Again freeze on the specimen Select new section thickness reduce the specimen Select a deeper temperature Wait until the knife and or the anti roll plate have reached the corresponding chamber temperature MICRON Sections do not stretch although the correct temperature has been selected and the anti roll plate has been adjusted correctly Sections break fissures in the sections sections are not stretched properly Sections roll over the anti roll plate Scratching noise while cutting and during the return travel of the specimen clamping Thick thin sections Tight handwheel punctual during the entire movement No chamber illumination MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf Knife and or anti roll plate are not Edge of anti roll plate is damaged Blunt knife Specimen too cold Static charge draught Specimen not cold enough Spacious specimen Anti roll properly plate not adjusted Anti roll plate not aligned parallel towards the knife edge Incorrect clearance angle Blunt knife Anti roll plate does not project over the knife edge properly Anti roll plate projects over the knife edge too much and scratches on the specimen Knife sharpness Knife angle Clamping on knife carrier Clamping on specimen holder Debris and section waste between microtome and base plate Soiled link block Toothed
67. hen using the motorized cutting drive a regulated D C motor works on the handwheel of the cryostat microtome by means of an electromechanical clutch and a reduction gear The speed of the motorized drive can be pre selected continuously on the operating controls from 0 to 250 mm s The selected speed will be carried out in the cutting window Outside the cutting window a higher return speed is determined by the electronic control unit Use either the operating controls or the foot pedal to turn on off the drive The limits of the cutting window can be changed continuously and can be adjusted to the specimen This can be done in an automatical mode or also by manual settings Three operating modes for the motorized cutting drive are available When in operating mode interval stroke the cutting drive only moves as long as the button START STOP or the foot pedal is pressed The movement can be stopped in any position When in operating mode single stroke the cutting drive moves from the starting position to the next lower reversal point When in operating mode continuous stroke an unlimited number of sections is carried out To stop the continuous stroke press the button START STOP or the foot pedal The cutting drive will stop after reaching the next lower reversal point In case of danger the motor drive can be stopped in every operating mode by pressing the emergency stop button or the emergency stop in the foot pedal Co
68. ig 76 1 and remove the spacer strip fig 76 8 For this push in the stop bolt fig 76 11 and turn the clamping lever fig 76 1 in a counter clockwise direction over the stop bolt Remove the clamping lever fig 76 1 together with the eccentric bolt First remove the clamping plate and then the spacer strip Fix the clamping plate with clamping lever fig 76 1 again in the blade carrier in reverse order To insert the blade now loosen the clamping lever fig 76 1 Note Press the clamping plate on its lower area to open the slot behind the clamping plate Insert the blade from the left side into the slot behind the clamping plate fig 76 9 as far as possible Tighten the clamping lever fig 76 1 Thus fixing the blade in its position If the cutting area of the blade is no longer usable the blade carrier can be moved The blade itself is clamped solidly into the blade carrier The complete carrier is moved Open the clamping lever for the upper part of the carrier fig 76 2 and move the blade together with the upper part to the left or right side Tighten the clamping levers fig 76 2 to fix this position Caution To avoid the danger of injury on the blade during adjustment of specimen always position the knife guards fig 76 7 over the blade edge MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON HM 550
69. iles Fig 75 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series Inserting the knife The knife is inserted into the knife carrier either from the side or from the front independently if one or two clamping plates fig 75 1 have been removed Loosen the clamping screws fig 75 2 on the clamping plates fig 75 1 Turn the turning lever fig 75 4 in a clockwise direction to tilt the anti roll plate fig 75 3 forwards Insert the knife Turn the turning lever fig 75 4 in a counter clockwise direction to put the anti roll plate fig 75 3 against the knife Then tighten the clamping screws fig 75 2 to fix the knife in its position If the cutting area of the knife is no longer usable the knife can be moved Open the clamping screws fig 75 2 and move the knife to the left or right side as required Fix the knife again via the clamping screws fig 75 2 Caution AN To avoid the danger of injury on the knife during adjustment of specimen always position the knife guards fig 75 7 over the blade edge Height adjustment of the knife Loosen the clamping screws fig 75 2 Via the knurled screws fig 75 5 which are placed on the left and right side of the knife carrier adjust the height of the knife to the position of the anti roll device Note Please note the parallel alignment of knife edge and edge of the anti roll
70. it with its controlling elements is also part of the system Stretching and disposing of is controlled by the cutting window that can be set on the microtome cryostat The suction vacuum can be set via an turning knob on the microtome cryostat MICRON Option D The option D which is used in the HM 550 is a fumigation unit for the application of disinfection media onto the operation areas in the cryo chamber which are accessible for the user during the intended use Warning To guarantee the functionality and safety of the fumigation unit only disinfection media must be used which do not contain flammable substances as solvents Therefore we recommend to use disinfection media based on water MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON HM 550 Series 1 2 TECHNICAL DATA HM 550 SERIES Specimen temperature control cece eeeeee cece eeeeeeee ee eeeeeeceeeeeeeaaeeeeeeaaeeeeeeaaaeees 10 C to 50 C 10 C to 35 C down to 35 C down to 60 C Defrosting automatic defrosting adjustable with temperature control manual immediate defrosting Microtome Section thickness range eseeeeieeerreeeerrreeerreees 1 500 um Fine section thickness range ccceeeeeeeneeeeeeeeeeeees 1 100 um Resolution sieer ea ea Tuei 1 um for 1 10 um 2 um for 10 20 um 5 um for 20 70 um 10 um for 70 100 um Specimen retraction during return travel Vertical specime
71. ith the Peltier element it is not guaranteed that the automatic approach system functions perfectly For the specimen temperature must not be lower than 35 C when activating the ACA In case the temperature is lower than 35 C it is recommended to insert the chuck into the specimen clamping and wait until the specimen temperature has adjusted to the chamber and or set specimen temperature The automatic approach system uses the intrinsic capacity of the specimen to notice a first contact between specimen and knife edge If specimen and knife edge get in contact with each other a very low current is measured via a pre amplifier which is placed directly behind the specimen holder The signal form of the detected current is transferred via the outlet of the pre amplifier to the micro processor A corresponding software recognizes the threshold value causing an immediate interruption of the horizontal drive The threshold value is also determined regarding the temperature The optimal function of the automatic approach system is based on the fact that the knife is conductively connected with the knife carrier base whereas the specimen holder is separated by an isolating piece MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 3 5 OPTION M MOTORIZED CUTTING DRIVE The motorized cutting drive facilitates routine work and ensures an even cutting speed also for harder specimens W
72. ith the selected settings Note If there is no time to reset the internal counter for the micro filter it is possible to ignore the message CHANGE MICRO FILT for the moment For this press the scroll enter button fig 98 8 However this means that the counter must still be reset This message will appear on the display every 24 h Note It is also possible to check if the filter must be replaced For this press the menu button fig 98 9 until the message MICRO FILT gt OK appears on the display However if the message MICRO FILT appears on the display the filter must be replaced MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 98 MICRON 2 19 3 SETTING THE SUCTION WINDOW The size of this zone is shown in millimeters within the symbol for the suction window When passing the suction window this symbol is shown inverted on the display During this automatic function the cutting window adjusts itself to the size of the specimen Note Cutting and suction window are identical Manual setting The suction window is determined by manual entries For this turn the handwheel so that the lower edge of the specimen is positioned slightly above the knife edge Press button fig 100 1 to set the upper limit of the suction window Tontinue turning the handwheel clockwise to place the upper edge of the specimen just below
73. l guide and correct it if necessary Carefully remove frost from front and rear part of the knife and from the anti roll plate i e with ethanol acetone or the like Tighten all clamping screws and clamping levers on the knife carrier and specimen holder Select the appropriate chamber and specimen temperature according to the specimen Note If the specimen was frozen with liquid nitrogen or similar freezing techniques the specimen must adapt itself to the cutting temperature Carefully select the required knife material and profile Adjustment of knife height Adjustment of proper clearance angle Select a clearance angle of 812 according to the facet angle of a steel knife Select a proper cutting speed The harder the material the slower the cutting speed Take care in bringing specimen and knife together The most protruding point of the specimen must be recognized for the optimal operation of the automatic approach system The specimen must not be covered at all or too thick by freezing medium MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON HM 550 Series Moreover for the functionality of the automatic approach system and the automatic window a certain conductivity must be given via the specimen chuck as well as from the knife edge to the microtome base For this reason the original equipment such as knife carrier an
74. ll still be valid To quit the submenu press the menu button fig 60 9 Now continue working with the selected settings The interrupted defrosting is shown in the status line on the display fig 61 as INTR Note A cancelled or interrupted defrost cycle must be repeated later on as otherwise the evaporator will cover completely with frost and cannot cool anymore see immediate defrosting part 2 11 2 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf SPECIM 725 CHAMBER 20 COUNTER FINE 3 HM 550 Series Fig 59 Fig 60 Fig 61 MICRON For this reason the function INTERRUPT is automatically reset at the end of an interrupted defrosting max time approx 1 h i e the next defrosting is carried out again at the pre selected defrosting time Note While INT is active neither the defrosting time nor the real time can be changed When INTR ACT appears in the status line this means that INTERRUPT was selected while defrosting should otherwise be carried out and the next defrosting is carried out again at the next pre selected defrosting time MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 2 11 4 EMPTYING THE DEFROSTING LIQUID Note The liquid from the daily defrosting process is collected in the container fig 62 1 Please note that this container is emptied in due time according to the safet
75. menu is also chosen and will still be valid To quit the submenu press the menu button fig 95 9 Now continue working with the selected settings Note If there is no time to reset the internal counter for the coarse filter it is possible to ignore the message CHANGE COARSE FILT for the moment For this press the scroll enter button fig 95 8 However this means that the counter must still be reset After further sections the message will appear on the display again every 1000 um Note It is also possible to check if the filter must be replaced For this open the submenu coarse filter in the menu If the message COARSE FILT gt OK appears on the display the entire sum of sections of 50 000 um has not yet reached However if the message COARSE FILT appears on the display the filter must be replaced MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 94 Fig 95 MICRON Replacing the micro filter Depending on how often the instrument is used the micro filter must be replaced at regular intervals however at least every 6 months If the internal software detects that the micro filter must be replaced the message CHANGE MICRO FILT appears on the display For this remove the cover for the micro filter fig 96 3 which is placed beside the coarse filter Pull off upper and lower connecting pieces
76. must be guaranteed that the instrument does not move in an uncontrolled way Now he cryostat can be rolled to its site of installation For possible return shipments please keep the packing MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series Warning The instrument must only be moved in standing or slightly tilted approx D position Choose installation site that nough ventilation for the cooling system is guaranteed the distance between wall and rear panel is approx 15 cm For this install the distance bolts the suction areas on either side are kept free the mains switch for separating the instrument from the power supply is accessible any time Moreover the installation site must be free from Mraught by open doors or by air conditioning systems Mirect exposure to sunlight into the cooling chamber MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON Note Both measures reduce the formation of frost and therefore result in more favourable work conditions A high air moisture as well as high ambient temperatures reduce the maximum performance of the instrument To fix the complete unit tighten the screws fig 6 1 by using the attached tool Afterwards df the instrument is equipped with the option M connect the attached foot pedal to the socket fig 7 3 on the rear panel of the instrument Note T
77. n power 8 highest suction power Note The user sets the desired value depending on the desired function stretching or suction process section thickness and size of the specimen Note Fig 92 The applied vacuum for stretching sections and or disposing of section waste is only active during the cutting window representing also the suction window Outside the cutting window the selected vacuum is turned off by means of a valve Setting the suction window is carried out in the same way as the cutting window MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 19 2 INSERTING AND REPLACING THE FILTER UNIT When the suction power and suction window are set in a correct way the section to be disposed of gets via the suction slot in the knife carrier into the filter unit consisting of a coarse filter for collecting section waste and a micro filter for filtering the air Section waste is collected in the coarse filter This coarse filter must be replaced depending on the volume of waste The coarse filter is actively cooled The micro filter must also be replaced regularly however at least every 6 months Removing coarse filter Press the bow fig 93 2 of the coarse filter downwards either manually or via the foot The coarse filter tilts forwards Remove the coarse filter by means of the attached clamp for filter replacement Replacing the coarse filter The c
78. n stroke Horizontal specimen movement Read outs Graphical LC display section thicknesses section counter sum of section thicknesses remaining travel to front end position size of the cutting window chamber temperature specimen temperature and graphic of temperatures menu with submenus Specimen approach automatic function alternatively manual with variable speed Size of chucks 30 40 mm special sizes upon request Specimen orientation X and y AXES Lacee universal 8 with zero positioning Z ARE areae a ETETA eae AARTEEN EE iA up to 360 Coarse feed motorized graduated single and continuous Cooling chamber illumination e eects eeeeeeeeeeeeeeeeeeeeeeee ee with variable illumination position Sliding window heated Option M Cutting drive manual and motorized electronically controlled Cutting window cutting window adjusted to specimen size manually Operating modes interval single and continuous stroke Cutting speed 0 250 mm s All temperatures refer to an ambient temperature of 20 C Cont d on page 2 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series 1 2 TECHNICAL DATA HM 550 SERIES Handwheel brake electromagnetic in any position Emergency stop via handswitch or foot pedal Vacutome max specimen size max section thickness to be disposed of max section thickness to be stretched min section thickness to be stretched Clearance
79. nal GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 4 5 1 Problem Cryostat temperature cannot be Ambient temperature too high achieved Cryostat is influenced by near heat generating instruments Draught into the cryostat Soiled condenser HM 550 Series POSSIBLE SOURCES OF ERRORS CAUSE AND REMOVAL Removal Lower ambient temperature by fresh air climate Please note the specified temperature of 22 C Change site of installation Change site of installation Open the service door on the left side clean the condenser LE cooling Call a service technician a built up on chamber wall Draught open doors windows Change site of installation close and on microtome windows doors Retarded specimen the Surface of the fast station is soiled freezing on of freezing Lower part of the chuck is soiled or damaged Chatter while cutting Unfavorable clearance angle Insufficient knife sharpness Knife not adequately clamped Chuck inadequately clamped Specimen inadequately frozen on onto the chuck Specimen grossed too thick it loosens from the chuck hard Specimen very and inhomogeneous Sticky sections Specimen not cold enough Knife and or anti roll plate are not yet cold enough section melts MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf Remove debris Remove debris remove damage Readjust clearance angle Move the knife in the knife carrier C
80. ng languages Cat No German 387747 English 387748 French 387749 Spanish 387750 MICROM International GmbH Robert Bosch Sir 49 D 69190 Walldorf MICRON HM 550 Series INTENDED USE Dear Customer Before putting the instrument into operation please read these operating instructions carefully to familiarize yourself with its proper operation and functions The MICROM cryostat series HM 550 are highly efficient cryostats for sectioning techniques in routine and research Only skilled or specially trained personnel must operate the microtome cryostat i e placing the specimen onto a chuck sectioning and transferring sections onto a slide The listed and marked safety measures as well as the regulations and hygiene measures of your respective lab must strictly be observed MICROM Ser No tireu a AA A E Please check the MICROM Ser No on the type plate which is placed on the rear side of your instrument and enter this number here This way questions and service can be handled faster Instruction Manual No 387748 Issued February 19 2004 MICROM International GmbH Robert Bosch Strasse 49 D 69190 Walldorf Telefon 06227 836 0 Telefax 06227 836 111 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON Intended HM 550 Series Use Table of Contents EC Certifi Safety Pr PART 1 1 1 1 2 PART 2 2 1 2 2 2 3 2 4 2 4 1 2 5 2 6 1 2 6 2 2 6 3 2 7
81. nt is equipped with an immediate defrosting function To avoid condensation the sliding window is heated when closed MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 3 2 CUTTING MOVEMENT The microtome of this cryostat is a rustproof rotary microtome The cutting movement is carried out by turning the handwheel The rotary movement results in the vertical and horizontal movement of the specimen clamp Sectioning is carried out by knives or blades which must be adjusted and fixed on the knife carrier With the downward movement of the specimen sectioning is carried out cutting movement The upward return travel of the specimen is carried out by turning the handwheel further in a clockwise direction During this return travel the specimen clamping is drawn back automatically to protect knife and specimen If required the function retraction can be turned off The selected section thickness is delivered at the upper reversal point of the return travel The number of the sections made can be shown on the section counter on the display After each downward movement of the specimen holder the number on the section counter increases by 1 The sum of the sections carried out can also be seen on the display For this trimming values and sectioning values are added up Section counter and sum of section thicknesses can be reset to zero at any time by means of the RESET button Mo
82. nt to a certain extent until the operational fault is removed by a trained service technician ERROR CODE NO END POSITION Before turning on the instrument on the main switch it carries out an automatic travel calibration of the specimen clamping The specimen clamping is brought into that position which has the longest distance to the knife carrier If there is an operational fault during this process e g debris or frost impedes the specimen clamping movement this is shown via the message NO END POSITION and means that a calibration was not carried out A service technician must be called SYSTEM ERROR The instrument is designed to check for internal system errors In case such an error is recognized it is shown as ER 10 to ER 16 for approx 5 sec in the middle of the display The recognition of such an error leads to an automatic RESET process The cause for this might be a temporary fault Note In case one of these messages occur more than once please note down the error code and contact a service technician MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 2 23 2 ERROR CODES OF THE OPTION D In case a malfunction occurs during a cycle error codes are shown on the display As different phases of the cycle might be concerned error codes are shown from step 1 to step 8 fig 127a Only step 3 is relevant for the operator This error shows that
83. ntinue sectioning pull out the emergency stop button or unlock the emergency stop in the foot pedal MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 3 6 OPTION VACUTOME STRETCHING SECTIONS AND DISPOSING OF SECTION WASTE The compression of sections when gliding onto the blade is a well known phenomena during sectioning in general and especially during cryo sectioning However this can be enormously reduced by using a Vacutome with a special blade carrier For this a vacuum can be set below the vacuum anti roll hood via an operating knob This vacuum anti roll hood acts as a traditional anti roll guide By means of the suction vacuum prevailing in the slot between vacuum anti roll hood and blade edge the cut is drawn on the air cushion over the blade thus immensly reducing the compression The section remains on the blade in stretched form The vacuum for stretching sections is turned off immediately after reaching the end of the specimen via an in line valve however depending on a correct setting of the cutting suction window Turn the vacuum anti roll hood backwards to transfer the cut onto a slide The Vacutome is also highly suitable for the disposal of debris For this the vacuum anti roll hood is used as described above When the anti roll hood is put on an air stream is generated in the suction window Continue cutting and the penultimate section is pushed forwards
84. o chamber from all section waste after Remove all tissue specimens brushes and chucks from the chamber Swivel the anti roll guide upwards Remove the two section waste trays Manually clean and disinfect them according to the regulations in your lab In case the cryostat HM 550 is equipped with a Vacutome use this device to dispose of the section waste into the coarse filter already while sectioning Biohazard AN To guarantee an optimal efficiency of the disinfection medium all surfaces must be accessible Moreover objects which do not belong to the instrument must not be inserted 3 Closing the window Always close the window before starting a run In case the window was not closed the following message appears on the display Please close the window Note Please close the window when leaving the instrument for the daily and or weekly mode Note A locking mechanism secures the closed window against opening MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON HM 550 Series 2 22 2 INTERRUPTING THE OPTION D The cycle can be interrupted at any time Press the reset button fig 113 8 Press the enter button fig 113 10 within three seconds to confirm the interruption process Warning Depending on the cycle phase when it has been interrupted the cooling system needs between 1 4 h to cool down to the programmed tempera
85. oarse filter must be replaced depending on the volume of the existing section waste If the integrated counter detects that the sum of all sections exceeds 50 000 um after the last resetting the message Change coarse filt appears on the display For this remove the coarse filter as described above Use lab gloves Dispose of the coarse filter together with the section waste according to the respective lab regulations Note The coarse filter is to be understood as a disposable filter and must not be used again Insert new coarse filter and close the filter MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 93 MICRON The internal counter for the coarse filter must be reset For this press the menu button fig 95 9 This way you get into the list of submenus Via the arrow buttons fig 94 8 and 94 6 select coarse filter This submenu is now shown inverted Via the enter button fig 95 10 select this submenu and simultaneously open it Via the arrow buttons fig 94 5 and 94 6 reset the sum of sections since the last resetting to 0 um Via the enter button fig 95 10 confirm the setting Note You return immediately to the list of submenus If the setting is not confirmed via the enter button you will quit the submenu automatically after five seconds and you return into the list of submenus The current setting of the sub
86. of the used examination materials and all special conditions for their processing pre treatment and if necessary storage as well as instrument controls for correct and safe operation is in the responsibility of the operator The operator is also responsible for special equipment and materials and or reagents for the operation of the instrument Warning Before turning on the instrument for the first time please check if the power requirements indicated on the type plate fig 8 correspond to the power supply voltage being used No other instruments should be connected to the circuit used for the cryostat as the compressor needs high surge currents when started Do not use multi socket power outlets with small wire sizes for the supply of the instrument Connect the power line of the instrument to the power outlet df the instrument is equipped with the option M plug in the foot pedal into the respective socket fig 7 3 Note Only for option M If the foot pedal is not connected there is the operating mode emergency stop see part 2 18 6 This way the handwheel brake is activated and the cutting drive motor cannot be started Always connect the foot pedal if the instrument is equipped with option M Turn on the power switch fig 7 1 on the rear side of the instrument Tlose the heated sliding window fig 6 2 and cool down the instrument MICROM International GmbH Robert Bosch Str 49 D
87. oke SINGLE is shown on the display Press the two start buttons fig 85 3 and 85 4 or the foot pedal once to release a single cutting cycle 7he movement stops in the lower reversal point Interval stroke Press button fig 85 1 until the operating mode interval stroke INTERV is shown on the display The cutting drive only moves as long as the two start buttons fig 85 3 and 85 4 or the foot pedal are being pressed MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf SPECIM CHAMBER COUNTER FINE 3 225 720 0 HM 550 Series 21 Fig 84 Fig 85 MICRON 2 18 3 SETTING THE CUTTING SPEED The desired cutting speed is set on the operating knob fig 86 2 Note To save time the return travel speed is enhanced in relation to the cutting speed especially for slow cutting speeds 2 18 4 START AND STOP OF THE CUTTING DRIVE Start Press the two buttons fig 86 3 and 86 4 of the safety switch either at the same time or briefly one after the other As an option the motorized cutting drive can also be started via the foot pedal 7he LED in button fig 86 3 lights up while the motorized cutting drive is active Note To start the motorized cutting drive the functions handwheel brake and emergency stop must not be activated The course function of the cutting drive results from the selected cutting window the selected operating mode and the
88. or in the rear part of the microtome chamber is defrosted automatically It is advisable to set the time of the defrosting cycle not during routine working time see part 2 11 1 According to the frost built up a defrosting cycle normally takes approx 40 min Immediate defrosting can be carried out at any time fig 58 9 For this press the menu button fig 58 9 Via the arrow buttons fig 57 5 and 57 6 select the submenu request defrost This submenu is now shown inverted Via the enter button fig 58 10 confirm this submenu and simultaneously open it Via the arrow buttons fig 57 5 or 57 6 choose ON or OFF If ON is confirmed request defrost is shown in the info line To confirm this setting press the enter button fig 58 10 Note You return immediately to the list of submenus lf the setting is not confirmed via the enter button you will quit the submenu automatically after five seconds and you return into the list of submenus The current setting of the submenu is also chosen and will still be valid To quit the submenu press the menu button fig 58 9 Now continue working with the selected settings Note Before starting the immediate defrosting it is absolutely necessary to remove the section waste as well as the tissue Warning Remove the specimen from the chamber when defrosting is carried out The temperature inside t
89. ped with a three wire ground plug The power outlet must be connected to the protective earth and must meet the International Electrotechnical Commission IEC regulations CAUTION MAINS VOLTAGE Never remove instrument covers during operation Component replacements as well as adjustments must only be made by trained service personnel Unplug the unit before removing or opening the covers DANGER IN EXPLOSIVE ENVIRONMENT The instrument must not be operated in the presence of flammable gases HAZARD OF FROSTBITE Avoid permanent touching of metal parts inside the cryostat microtome chamber as frostbite may occur at unprotected hands and arms HAZARD OF RADIOACTIVE RADIATION AN When working with radioactive specimens observe all applicable radiation safety procedures When working with radioactive contaminated material appropriate safety and disinfection measures must be carried out According to the rules and regulations concerning the handling of radioactive contaminated material of the respective laboratory safety clothing e g particle mask gloves protective shoe covers must be worn Radioactive contaminated waste must be disposed of according to the respective regulations MICROM International GmbH Robert Bosch Sir 49 D 69190 Walldorf MICRON HM 550 Series HAZARD OF INFECTION Use the appropriate safety and disinfection measures when working with infectious specimens According to the rules and regulations concerning
90. reover the remaining travel to the front end position can also be shown on the display The remaining travel which is still available for sectioning is shown in microns When the specimen clamping is in the back end position the display shows 28 000 um This number decreases the further the specimen clamping is moved towards the front i e in the direction of the blade MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON HM 550 Series 3 3 SPECIMEN COARSE FEED AND TRIMMING FUNCTION After changing the specimen or moving the knife or knife carrier it is necessary to adjust the knife edge to the specimen again This can easily be done by means of the coarse feed and the trimming function After the specimen and the knife are adjusted further gradual feed for trimming can be carried out by pressing the button for trimming If this function is chosen the specimen clamping moves forwards by the pre selected trimming value whenever pressing the button for trimming MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 3 4 AUTOMATIC APPROACH SYSTEM The automatic approach system is used for the fast and exact approach of the specimen towards the knife edge The function of the automatic approach system is limited to the temperature range between 5 C and 35 C Caution In case the specimen was frozen on to the chuck with CO gas liquid nitrogen or w
91. rizontal travel Automatic approach system for exact and safe approach of specimen towards the knife edge from 5 to 35 C Section counter and indication of sum of section thicknesses with reset button Indication of remaining travel MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Handwheel brake in any position Fine orientation of specimen with zero device Rotatable on Zaxis 360 Basic outfit with tree specimen chucks 118 ml MICROM freezing medium 100 ml cryostat oil brush shelf and section waste tray consisting of two parts Option M Electric motor drive with electronic control of the cutting stroke Cutting speed variable from 0 to 250 mm s Accelerated return travel Freely selectable cutting zone with automated function Three modes of operation interval single and continuous stroke Indication of handwheel brake Manual control of motor drive via safety start procedure Stop command selection via manual control foot pedal or handwheel brake Emergency stop key and foot pedal emergency stop Option V Vacutome system for stretching cryo sections as well as for the disposal of sections when trimming The system consists of a duo filter system The coarse filter collects the section waste The micro filter with a 99 99 filtration efficiency for 0 1 um particles filters the air The coarse filter is actively cooled in its position in the cryostat A vacuum generating un
92. rnational GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 17 OPTION P SPECIMEN COOLING If the cryostat is equipped with a specimen cooling the chamber as well as the specimen temperature is shown on the display fig 79 2 17 1 ACTUAL AND SET VALUE OF THE SPECIMEN TEMPERATURE The specimen cooling of the cryostat is set via the corresponding part on the operating controls fig 80 2 The actual and set values of the specimen cooling are shown in C on the display fig 79 On the right side of the display the actual value is also shown graphically Press button fig 80 2 for specimen temperature 7SPECIMEN is then shown inverted The set value settings are made via the up button fig 80 5 Mown button fig 80 6 Note The valid range of the set value goes from 10 C down to 50 The actual value of the specimen temperature is shown as actual temperature and as a graphic After having chosen the requested values the display automatically returns to its basic indications after three seconds i e fine section thickness is again shown inverted MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series HM550 OMV SET ACT SPECIM 25 24 M CHAMBER 20 21 COUNTER Fig 79 Fig 80 MICRON 2 17 2 SPECIMEN ORIENTATION WITH ZERO DEVICE with option O In many cases the orientation of the specimen in relation to the cutting edge woul
93. rozen from above and below at the same time Thus the freezing time can be reduced Moreover the formation of artefacts can also be reduced considerably dnsert the chuck into the fast freezing rail for standard version or into the rail with Peltier element for versions with option P Then insert a cryo mold fig 20 2 onto the chuck 30 or 40 mm Fill freezing medium into the mold and place the specimen onto the freezing medium The heat extractor is placed horizontally onto the specimen Note Use a frozen heat extractor MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 20 MICRON HM 550 Series 2 7 FEED 2 7 1 SPECIMEN COARSE FEED For the fast forward and backward travel between knife and specimen the cryostat microtome has a motorized coarse feed system The approach between specimen and knife can also be carried out by means of an automatic function see part 2 7 4 Return travel of the specimen clamping Press button fig 21 4 Pressing the button 3 briefly leads to short return travels only Press the button fig 21 4 for more than approx 2 seconds the function is carried out Fig 21 continuously The specimen clamping will entirely be moved backwards Note The rear end position is defined as that position which has the longest distance to the knife To stop this function briefly press button fig 21 4 once more When the r
94. rried out at the site of installation If this is not possible for some special reasons the instrument can be returned to MICROM The contact address can be found at the beginning of this instruction manual To guarantee trouble free function of the instrument after transportation please note the below mentioned measures for the transportation preparation In addition the conditions for storage and transportation as mentioned in part 1 2 must be observed during the entire transportation Biohazard Please also note the precautionary VEN measures described in our safety precautions concerning biological hazards Measures for closing down Turn off the instrument Unplug the unit Remove blade and or knife from blade and or knife carrier Remove section waste trays brush shelf blade knife carrier and other accessories as well as tools from the cryo chamber Before the transportation empty all containers for defrosting liquid and disinfection solution to avoid the unintended release of the fluids When removing the containers please also note the precautionary measures which are described in the chapter Safety Precautions Note Clean and disinfect these accessories according to the respective applicable lab regulations and transport them in dry condition MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series To remove the container for the disin
95. sch Sir 49 D 69190 Walldorf MICRON HM 550 Series SAFETY P WARNING RECAUTIONS SIGNALS AND SYMBOLS The installation and routine use of the HM 550 Series is easy and safe if the instructions in this manual are being observed Note Special instructions regarding operation of the instrument Warning Special precautionary measures to prevent damage to equipment For a long lifetime of the equipment please observe these instructions carefully Caution general danger spot The instruction manual must strictly be observed whenever this symbol is visible on the instrument Biohazard Warning of biological danger Radioactivity Warning of radioactive danger Chemicals Warning of unhealthy or irritating substances MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series SAFETY PRECAUTIONS CAUTION The operator s safety is affected when the instrument is not operated in accordance with this instruction manual Please observe the following general precautions during operation of this instrument Failure to comply with these precautions violates safety standards and the intended use of the instrument MICROM International GmbH is not liable for misuse of the instruments and failure to comply with basic safety requirements INSTRUMENT GROUNDING To avoid injury from electrical current the instrument must be connected with the protective earth The instrument is equip
96. se observe the filling level The red stopper must be inserted after cleaning as otherwise cold air leaks out during cooling operation Thus generating frost built up The installation and re setting up of the instrument is carried out in reverse order MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 5 3 CLEANING AND CARE OF THE MICROTOME Carefully clean and dry the dismounted microtome Carefully clean and dry the dismounted knife carrier as well As there is condensated humidity inside the microtome dry the microtome components inside the cooling chamber very carefully For this the microtome can be treated in a drying closet at temperature up to 60 C After each shutting off or cleaning of the cryostat the cross roller bearing should be lubricated By means of a pipette fig 133 3 the cross roller bearings fig 133 4 can be reached from an angle from behind below the housing fig 133 1 Fill the pipette with a small amount of cryostat oil cat no 350040 Put one or two drops into the space fig 133 2 of the cross roller bearings The specimen clamping should be in the lower position Also slightly lubricate the horizontal cylinder guide behind the specimen clamping Note For the examination and re adjustment of the microtome a routine maintenance should be performed by trained service technician once a year Cleaning and
97. section waste trays brush shelf blade knife carrier and other accessories as well as tools from the cryo chamber Note Clean and disinfect these accessories according to the respective applicable lab regulations and transport them in dry condition Clean and disinfect the cryo chamber according to the respective applicable lab regulations Unscrew the handwheel handle for transportation Note To make sure that these is no condensation water inside the chamber let the instrument approx 48 h dry Arrange the dismounted accessories into the intended transportation packing Note Before the further transportation tighten the fastening screws so that the instrument does not move during transportation MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 6 2 DISPOSAL OF THE INSTRUMENT AFTER FINAL SHUTDOWN After the final shutdown of the instrument we recommend to contact a local recycling company for the disposal according to the national applicable regulations MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series
98. set cutting speed please see part 2 18 3 Stop in operating mode continuous stroke Press one of the two buttons fig 86 3 or 86 4 or press the foot pedal 7he LED fig 86 3 goes off MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf U HM 550 Series Fig 86 MICRON HM 550 Series 2 18 5 HANDWHEEL BRAKE Unintended movements of the specimen holder can be avoided via the handwheel brake This educes the danger of being injured while adjusting specimen clamp and knife carrier Warning When the instrument is turned off the handwheel brake is not operative For your personal safety the handwheel brake should be turned on when working on the specimen holder or knife carrier w a nl Warning 3 Activating the brake Stop the motorized cutting movement via Fig 87 buttons fig 87 3 and 87 4 7hen activate the function handwheel brakevia button fig 87 6 The red LED fig 87 7 lights up The motorized cutting drive cannot be started by mistake anymore Releasing the brake To release the handwheel brake press button fig 87 8 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 18 6 EMERGENCY STOP To quickly eliminate danger the microtome is equipped with two emergency stop functions Caution In case danger arises from cutting drive activate the emergency stop Hand emergency stop The hand emergency stop
99. side of the base Again tighten the clamping lever This way the set clearance angle is fixed in its position Note Usable cuts are only achieved at clearance angles between 24 30 Moving the magnetic blade carrier To move the magnetic blade carrier loosen the lever on the right side of the base plate To fix the blade carrier in its new position tighten the lever again Fine adjustment of the vacuum anti roll hood The vacuum anti roll hood fig 1034 can be removed and put on by using the turning lever fig 103 5 The knurled screw fig 103 6 allows the adjustment of the edge of the vacuum anti roll hood fig 103 4 in relation to the blade edge The fine adjustment of the anti roll device is carried out via the knurled screw fig 103 6 Function of the Vacutome A closed vacuum anti roll hood fig 104 4 and a suitable suction vacuum generate stretching or suction effects For this the closed vacuum anti roll hood fig 103 4 slightly tilts the underneath flap fig 104 8 thus opening a suction slot fig 104 9 Lift the vacuum anti roll hood fig 104 4 the flap fig 104 8 beneath falls back in place and the slot gets closed MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON HM 550 Series 2 21 OPTION P ACTIVE DEEP FREEZING STATION WITH PELTIER ELEMENT To accelerate freezing on of a specimen on a PEL
100. splay press button fig 30 10 until this line is blank 2 8 1 SECTION COUNTER The section counter adds up the number of sections produced After each downward movement of the specimen holder the number on the section counter increases by 1 7he counter can be reset to zero via button fig 30 8 2 8 2 SUM OF SECTION THICKNESSES This value shows the sum in microns of the sections already cut 7 rimming values as well as sectioning values are added up This value can be reset to zero via button fig 30 8 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 30 SPECIM 725 CHAMBER 20 COUNTER 38 FINE 3 SPECIM CHAMBER SUM 1 500 CONTIN Fig 32 MICRON 2 9 3 REMAINING TRAVEL TO FRONT END POSITION This value shows the distance in microns which is left for sectioning When the specimen clamping is in the rear end position the display shows 23 000 um This number decreases the closer the specimen holder moves towards the front Note If no further feed is possible anymore the display automatically shows the remaining travel independently which information on the sectioning processes e g number of sections or sum of section thicknesses was chosen before 2 9 4 REAL TIME The real time is shown here MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series SPECIM 25 24 Zz CHAMBER 20 21
101. t HM550 OMVP SET heats up until it has reached a positive end temperature This way tissue specimens can be detached from the chuck inside the chamber fig 110 SPECIM 725 CHAMBER 20 DETACH ACTIVE COUNTER 0O j FINE 3 First put freezing medium onto an uncooled chuck Then put the specimen onto the freezing medium Afterwards the chuck together with the Fig 110 specimen is inserted with the peg into one of the four front fast freezing stations fig 111 These four front fast freezing stations are equipped with a Peltier cooling element Fig 111 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 22 OPTION D INTEGRATED AUTOMATIC UNIT FOR THE APPLICATION OF DISINFECTION MEDIA The option D which is used in the HM 550 is a fumigation unit for the application of disinfection media onto the operation areas in the cryo chamber which are accessible for the user during the intended use Spraying is carried out via the fumigation of disinfection solution by means of ultrasonics The droplets of the mist have a size of 1 3 um This very small size allows for an even application of the disinfection solution onto surfaces This process takes approx 5 6 h and several phases are processed Caution AN To guarantee the functionality and safety of the fumigation unit only disinfection media must be used which do not contain combustible and or flammable su
102. tant by means of a corresponding control unit The specimen can be detached directly on the specimen head Set specimen temperature to 0 C to 5 C inside the chamber The following two processes are also possible Active detach function in case the instrument is equipped with this accessory inside the chamber Cooling block with 6 stations for one chuck each outside the chamber MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series 3 8 OPTION D INTEGRATED AUTOMATIC UNIT FOR THE APPLICATION OF DISINFECTION MEDIA The option D which is used in the HM 550 is a fumigation unit for the application of disinfection media onto the operation areas in the cryo chamber which are accessible for the user during the intended use Warning To guarantee the functionality and safety of the fumigation unit only disinfection media must be used which do not contain flammable substances as solvents Therefore we recommend to use disinfection media based on water MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series 4 WORKING WITH THE CRYOSTAT 4 1 PREPARATIONS ON THE MICROTOME AND INSIDE THE COOLING CHAMBER Before sectioning the specimen clamping and the cryo chamber should be at a stable temperature around the desired cutting temperature Select a chamber and specimen temperature corresponding with the consistency of the specimen
103. tering of the blade This might be caused by an incorrect approach Caution When using different freezing techniques for example by means of the fast freezing stations liquid nitrogen CO and the like which generate a freezing temperature of below 35 C the automatic approach system can only be applied after the specimen will have adjusted itself to the respective temperature of the specimen holder which must be between the temperature range Starting the automatic approach Use the handwheel to make sure that the most protruding point of the specimen is opposite the knife edge This position must be within the selected cutting window Note If the button fig 25 1 is pressed twice and the cutting window is inactive the automatic approach can be released in any position Press button fig 25 2 to start the automatic approach process The red LED in this button fig 25 2 lights up and confirms the chosen function and AUT APPROACH is shown in the status line on the display fig 24 27he specimen clamping moves forwards until the knife edge touches the specimen MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series SPECIM 25 4 Zz CHAMBER 20 21 COUNTER 0O CONTIN FINE 3 TRIM 20 AUT APPROACH REN R Fig 24 Fig 25 MICRON 4mmediately afterwards this forward travel of the specimen clamping is stopped and moved back
104. ternational GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series MICRON 2 22 1 PREPARING AND STARTING THE OPTION D Note The sequence of the below mentioned steps must strictly be observed so that the automatic fumigation device can operate in an optimal way With regard to possible occurring irritating gases during the fumigation of disinfection medium into the cryo chamber it is absolutely necessary to care for sufficient ventilation at the site of installation 1 Filling in disinfection solution Fill the store reservoir on the rear side of the instrument with disinfection solution Before filing the reservoir please check the keeping quality and the safety data sheet of the respective disinfection medium Please also observe the handling storing and disposal instructions from the manufacturer of the respective disinfection medium Moreover please respect the instruction from the manufacturer concerning safety measures during an unintended release Caution For this please note the safety note in part 2 22 The max filing amount of the storage container is 1 which will be sufficient for seven cycles This way it is guaranteed that the expiring date of the disinfection solution inside the cryostat will practically never be passed MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series fig 112a MICRON 2 Cleaning the cryo chamber Clean the cry
105. the handling of infectious radioactive contaminated material of the respective laboratory safety clothing e g particle mask gloves protective shoe covers must be worn HAZARD OF BIOLOGICAL DANGER AN Specimens used during the intended operation of the instrument might potentially be infectious For this reason it is recommended to observe the general laboratory regulations concerning protection against danger of infection Information on decontamination media their use dilution and effective range of application can be read in the Laboratory Biosafety Manual 1984 of the World Health Organization HAZARD OF MALFUNCTION To avoid the hazard of malfunction of an instrument it must only be operated in a controlled electromagnetic environment This means that transmitters such as mobile phones must not be operated in their close vicinity In case of malfunctions and or service work please turn off the instrument and contact your local dealer CARE IN USING MICROTOME KNIFE A To diminish the danger of being injured by the knife or blade use the knife guard when adjusting specimen and knife If possible the specimen should be clamped in before the knife is inserted into the knife holder Before changing the knife holder always remove blade or knife Unused knives should always be kept in a knife case Never place the knife with the cutting edge upwards Never try to catch a dropping knife Never check the sharpness of the cutting ed
106. the knife edge Press button fig 100 1 to set the lower limit of the suction window 7he LED in button fig 100 1 lights up during each further passing through of the suction window The length of the suction window is shown in mm within the suction window symbol on the display Note A suction window should only be set while the specimen is moved downwards If by mistake a suction window limit is set during return travel of the specimen the set limits are applied to the cutting movement accordingly Note In case the suction window limits are set incorrectly please repeat above mentioned process again MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series SPECIM 25 42 Zz CHAMBER 20 21 COUNTER FINE 3 Fig 99 Fig 100 MICRON HM 550 Series 2 20 KNIFE CARRIERS FOR OPTION VACUTOME 2 20 1 DISPOSABLE BLADE CARRIER EV The disposable blade holder EV takes up all commercially available low profile blades with a dimension of 80 x 8 mm as well as high profile blades with a dimension of 76 x 14 mm an a facette angle of approx 35 Fig 101 Fig 102 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON HM 550 Series MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON Inserting the blade Insert the blade into the slot behind the clamping plate fig 101 1 Note When using high profil
107. there is insufficient disinfection medium in the container before a cycle can be started fig 112a After having filled the container the next programmed cycle will be carried out automatically Remove the error with the reset button The error steps 1 and 2 as well as 4 to 8 refer to service related malfunctions during a cycle Therefore please contact a service technician Behaviour of the software in case of power failure A power failure during the fumigation process is stored by the program After the power has been resumed the window is locked The display shows the following message which means that a cycle was interrupted CAUTION Cycle interrupted due to power failure or turning off of the instrument Continue with Reset button The window of the instrument remains locked to avoid possible annoyance caused by odours MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series DES01 OMVPD SOLL IST Fehler Schritt1 IIIIIIIIILELELELELI Weiter mit der Reset Taste Fig 127a MICRON HM 550 Series 2 24 ACCESSORIES 2 24 1 STANDARD EQUIPMENT The microtome cryostat HM 550 series is equipped with the following accessories Cat No 2 specimen chucks 30 mm round 715710 1 specimen chuck 40 mm round 715720 1 Allan key 2 5 mm 362220 1 Allan key 5 mm 362260 1 Allan key 6 mm 362120 1 flat brush 334170 1 brush 334170 1 cryostat oil no 407 100 ml 350040 1 freezing
108. tion machine In the rear area of the microtome chamber is an evaporator which the cold coolant flows through as well as through the specimen head Due to the heat extraction from the microtome chamber a cooling down of the microtome and or cryo chamber to 35 C can be achieved The temperature of the microtome and or chamber can be adjusted continuously down to 35 C For this pre select the desired temperature as set value on the control panel The actual value is constantly measured and sent to the control unit By means of an installed thermo electrical element controlled temperatures down to 50 C can be achieved on the specimen head On the left side of the microtome chamber there are freezing stations fast freezing stations which can be cooled down to a temperature of 35 C depending on the cryo chamber temperature This fast freezing station has 12 positions for one chuck each The instrument can optionally be equipped with an active deep freezing device The station can be cooled down to 60 C by means of a Peltier cooling element This Peltier cooling element has four positions for one chuck each The eight positions correspond to the conventional fast freezing stations The specimen temperature control serves for a more efficient working with the cryostat For various specimens different cutting temperatures can be achieved very quickly After having cut the specimen can be detached quickly from the chuck by usin
109. ture Only after having reached the pre selected values sectioning is possible again Fig 113 MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf MICRON 2 22 3 PROGRAMMING THE OPTION D Press the menu button fig 116 9 This way you get into the list of the submenus Select the respective submenus from this list via the arrow buttons fig 115 5 and 115 6 Mia the enter button fig 116 10 open the corresponding submenu Carry out the respective entries fig 115 5 and 115 6 Confirm with the enter button fig 116 10 Either quit the list of submenus via the menu button fig 116 9 or select another submenu via the arrow buttons fig 115 5 and 115 6 Within this list the following submenus for programming the cycle are important Submenu Defrost time Press the enter button fig 116 10 to select the submenu Enter the defrost time in this submenu see part 2 10 2 Setting the defrost time When the daily or weekly mode has been selected as described below the cylce starts at the pre selected defrost time Confirm the settings via the enter button fig 116 10 Note The last phase of each cycle is always a normal defrosting of the crostat Submenu Option D ON OFF Press the arrow buttons fig 115 5 and 115 6 to select the desired settings Confirm with the enter button fig 116 10 Note When selecting ON the Mode Option D
110. uttons only please note the following After having chosen a function its pre selected values can be changed via the up or down buttons However if no entry is made within five seconds the operating control switches back into its basic status MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series Fig 11 MICRON Reset button This button fig 12 8 is used to reset added values e g number of sections sum of section thicknesses to zero Menu button This button fig 12 9 is used to get into the submenus Basic settings times service functions and options are set and or called Enter scroll button Within the submenu basic settings are carried out further submenus are called and other functions are activated via this button fig 12 10 During operation of the instrument e g while cutting this button is used to choose between the indication of the section thickness section sum real time and indication of the distance to the end position of the specimen Keyboard lock To lock the keyboards and to block the feed movement press the buttons fig 12 8 and 12 10 for approx 2 sec The display fig 13 shows LOCKED To unlock the keyboards press the buttons fig 12 8 and 12 10 again Note When the keyboards have been locked this leads automatically after 5 sec to the standby status and after another 5 sec to the sleep status MICROM International GmbH
111. wards by a safety distance of 200 um AUT APPROACH is still shown on the display as the process of the automatic approach has not yet been finished When passing the next upper reversal point the specimen clamping automatically moves forwards again by 200 um 7he red LED in button fig 25 2 goes off as well as the term AUT APPROACH disappears 7he specimen is now in position to start sectioning Interruption of the automatic approach Press button fig 25 2 or ass upper or lower reversal point via handwheel When an automatic approach has been carried out another approach can be started immediately if needed This might become necessary when the specimen has been oriented again Error codes during an automatic approach process Error code AMPLIFIER ERROR is shown on the status line on the display in case the activating signal for the automatic approach has already been recognized before the automatic approach movement has been started Possible cause the specimen has already been in contact with the knife edge e g by protruding fibers Press button fig 25 2 remove the cause for the error and press button fig 25 2 again to start the automatic approach once more However if there is no obvious explanation for this error code e g frost built up please call a service technician Error code OUT OF TEMPERATURE appears in the status line on the display if the actual temperatur
112. y precautions at the beginning of this instruction manual We highly recommend to treat the container with a disinfection solution after having emptied it MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf HM 550 Series fig 62 MICRON HM 550 Series 2 12 CUSTOMER SPECIFIC SETTINGS Vers 1 00 3 MENU f f gt PELTIER STATION The information on the display can be shown in DEFROST TIME y A WAKE TIME various contrasts and in four different languages STANDBY DELAY REALTIME DATE REQUEST DEFROST 2 12 1 SETTING THE CONTRAST COARSE FEED SPEED CONTRAST FINE FILTER RESET To set the contrast higher or lower press the COARSE FILT RESET RETRACTION ENABLE menu button fig 65 9 This way you get LANGUAGE into the list of submenus pees Via the arrow buttons fig 64 5 and 64 6 select contrast This submenu is now Fig 63 shown inverted Via the enter button fig 65 10 confirm this submenu and simultaneously open it Several black stripes now appear on the display To set the contrast of the stripes lower constantly press the button down fig 64 6 To set the contrast of the stripes higher constantly press the button up fig 64 5 When the desired contrast has been achieved press the enter button fig 65 10 for confirmation Fig 64 To quit the submenu press the menu button fig 65 9 Now continue working with the sel

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