Home

USB-MEA256-System Manual

1. Stimulation Connector Socket 1 Stimulation Connector Socket 2 Stimulation Connector Socket 3 Stimulation Connector Socket 4 Electrode Stim Spring Hardware Electrode Stim Spring Hardware Electrode Stim Spring Hardware Electrode Stim Spring Hardware ID Socket Contact ID ID Socket Contact ID ID Socket Contact ID ID Socket Contact ID A2 2 1 196 19 60 191 125 C15 61 98 239 A10 25 113 236 B1 4 133 130 K7 7 38 41 C16 62 222 209 A11 22 232 205 B2 1 2 226 K8 34 178 74 D13 63 99 171 A12 15 108 169 C1 5 4 223 K9 33 51 104 D14 57 96 184 A13 12 227 143 c2 6 134 193 L10 56 189 2 D15 58 220 213 A14 5 103 241 c3 3 3 158 L11 59 64 30 D16 55 95 181 A15 4 223 141 D1 12 137 254 L7 11 40 42 E13 53 94 182 A3 62 252 195 D2 9 6 224 L8 31 50 35 E14 54 218 243 A4 55 128 159 D3 10 136 194 L9 36 179 5 E15 51 93 179 A5 52 247 131 E1 15 9 55 M10 46 184 69 E16 52 217 147 A6 45 123 229 E2 16 139 25 M11 49 59 100 F11 59 97 183 A7 42 242 202 E3 13 8 123 M5 8 165 14 F12 49 92 180 A8 35 118 166 E4 14 138 93 M6 18 170 78 F13 50 216 145 A9 32 237 138 E5 8 135 253 M7 21 45 105 F14 47 91 177 B10 26 234 206 F1 22 142 91 M8 28 175 7 F15 48 215 191 B11 19 110 170 F2 19 11 56 M9 39 54 33 F16 45 90 178 B12 16 229 144 F3 20 141 26 N10 43 56 34 G11 56 219 245 B13 9 105 242 F4 17 10 124 N11 50 186 70 G12 46 214 192 B14 6 224 211 F5 18 140 94 N12 53 61 97 G13 43 89 175 B15 1 101 240 G1 25 14 122 N13 63 66 27 G14 44
2. amp 666060606006660006068060 Se 2 e6666866600666060G000 e so amp 666060606006066000606060 Se ee e6006060606060606060060 99 E9 D ko i dm Sn a A Go do 0 Mo o do fo Oh amp d ee s 0606000000600000006 se um a do do m m o amp m m hh h m m er 6666600600G60066 a 2e BH MBARHRABHRBARHBHBAB 99 s B SSHSESECEHOSCESHE se x amp 566006 Y P13 B14 A14 un 19 A15 014 P14 R14 B16 815 N13 GND D13 015 Fit D14 D16 E13 E15 F12 F14 F16 G13 GIS H12 HIA H16 H10 111 115 113 K16K14 K12 L15 L13 M16 M14 Kf N15 K10 016 014 P15 GND C16 H9 D15 G11 E14 E16 F13 F15 G12 G14 G16 H13 HIS H11 110 116 114 112 K15K13 L16 L14 L12 M15 MIS N16 N14 M12 O15 P16 R15 The letter digit code is the electrode identifier and refers to the position of the electrode in the 16 x 16 layout grid The layout of the letter digit code for the four connectors of the USB MEA256 amplifier is shown To correlate the pin layout of the connectors please see the table on the next page Multi Channel Systems Fon 49 7121 9 09 25 O O 2014 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change multicl ice German y www multichannelsystems com without notice multichannel 256ThinMEA systems
3. Use the test model probe to test the amplifier If the noise level is fine without the MEA bad MEA electrodes cannot be the cause 24 5 5 5 6 Troubleshooting MEA is defective MEAs wear out after multiple uses or over a longer time of use for example for long term cultures This is considered a normal behavior MEAs are also easily damaged by mishandling for example if wrong cleaning solutions or too severe cleaning methods are used or if the recording area is touched If you observe a bad long term performance of MEAs consider a more careful handling Possible causes The contact pads are contaminated Clean the contact pads carefully with a swab or a soft tissue and pure 100 alcohol The contact pads or the electrodes are irreversibly damaged You could have a look at the electrodes under a microscope If they appear shiny golden the TiN is gone and the electrode is irreversibly damaged Electrodes may be damaged without changing their visual appearance though Pick one of the bad channels after the other and ground it See the MEA amplifier s manual for more information on grounding channels In most cases only one of the electrodes that appear bad is actually defective and the other ones are only affected by the single defective electrode Ground as many electrodes as you need for a good general performance Grounded electrodes show a noise level that is lower than that of good electrodes If too many electro
4. Warning Make sure to read the following advice prior to installation or use of the device and the software If you do not fulfill all requirements stated below this may lead to malfunctions or breakage of connected hardware or even fatal injuries Warning Always obey the rules of local regulations and laws Only qualified personnel should be allowed to perform laboratory work Work according to good laboratory practice to obtain best results and to minimize risks The product has been built to the state of the art and in accordance with recognized safety engineering rules The device may only be used for its intended purpose be used when in a perfect condition Improper use could lead to serious even fatal injuries to the user or third parties and damage to the device itself or other material damage Warning The device and the software are not intended for medical uses and must not be used on humans Malfunctions which could impair safety should be rectified immediately High Voltage Electrical cords must be properly laid and installed The length and quality of the cords must be in accordance with local provisions Only qualified technicians may work on the electrical system It is essential that the accident prevention regulations and those of the employers liability associations are observed Each time before starting up make sure that the power supply agrees with the specifications of the product Check the power cord
5. 1 e v ABCDEFGH IKLMNOPR v CO 2 Connector 3 Technical Specifications 256MEA Temperature compatibility 0 125 C Dimension W x D x H 49 mm x 49 mm x 1 mm Base material Glass Contact pads and track material Indium tin oxide ITO Electrode diameter 8 10 or 30 um nterelectrode distance centre to centre 30 60 100 or 200 um Electrode height Planar Electrode type Titanium nitride TiN solation type Silicon nitride SiN Electrode impedance 30 50 kQ for 30 um or 250 400 kQ for 10 um Electrode layout grid 16x16 Number of recording electrodes 252 Number of reference electrodes 4 internal reference electrodes iR Contact pads for reference electrodes connected to ground 4 MC Rack Source layout in Data Source Setup Channel map MEA perfusion chamber Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Configuration 16 x 16 cmp w o Without ring Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm r T Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 0 O 2014 Multi Channel Systems MCS GmbH Fax 49 7121 9 09 25 11 info multichannelsystems com Product information is subject to change www multichannelsystems com without notice multichannel 256MEA systems a 6 62 65 da d 9 D 5 Fh AAA i P 9 20 GG
6. 213 189 B16 2 100 210 G2 26 144 92 N5 14 168 77 G15 41 88 176 B3 61 131 225 G3 23 13 53 N6 17 43 108 G16 42 212 190 B4 58 250 198 G4 24 143 23 N7 24 173 10 H10 33 84 150 B5 51 126 162 G5 21 12 121 N8 27 48 38 H11 36 209 185 B6 48 245 134 G6 11 7 156 N9 40 181 6 H12 39 87 153 B7 41 121 232 G7 1 5 155 010 44 183 3 H13 40 211 187 B8 38 240 201 H1 32 147 21 011 47 58 31 H14 37 86 152 B9 29 115 233 H2 29 16 119 012 54 188 67 H15 38 210 188 C10 23 112 167 H3 30 146 89 013 57 63 98 H16 35 85 154 C11 20 231 139 H4 27 15 54 03 3 36 44 H9 60 221 215 C12 13 107 237 H5 28 145 24 04 10 166 80 110 34 208 186 C13 10 226 212 H6 31 17 51 05 13 41 107 111 31 83 151 C14 3 102 172 H7 34 148 90 06 20 171 9 112 28 205 59 C4 57 129 228 11 35 19 52 07 23 46 37 113 27 81 149 C5 54 248 197 12 38 150 87 08 30 176 73 114 30 206 62 C6 47 124 161 13 37 20 117 09 37 53 101 115 29 82 148 c7 44 243 133 14 40 151 19 P1 2 34 82 116 32 207 61 c8 37 119 231 15 39 21 49 P10 41 55 102 K10 7 71 63 C9 30 236 203 16 36 149 22 P11 48 185 4 K11 11 73 247 D10 24 233 140 17 33 18 120 P12 51 60 32 K12 21 78 250 D11 17 109 238 18 60 161 16 P13 58 190 68 K13 24 203 57 D12 14 228 207 K1 42 152 88 P14 61 65 95 K14 23 79 251 D4 63 132 157 K2 41 22 118 P2 1 35 112 K15 26 204 60 D5 53 127 227 K3 44 153 20 P3 6 164 79 K16 25 80 146 D6 50 246 200 K4 43 23 50 P4 9 39 110 L12 18 200 220 D7 43 122 164 K5 46 154 85 P5 16 169 12 L13 17 76 248 D8 40 241 136 K6 56 159 15 P6 19 44 40 L14 20 201 221 D9 27 114 16
7. 9 Analog Input A1 to A4 11 Analog input signals 9 AUDIO 11 C Cardiac signals 9 D DO IN 11 DO OUT 11 Data acquisition 1 9 11 DIGITAL IN OUT 11 Digital IN OUT Extension 20 E Electrode Stimulation 20 F Faraday cage 9 Field potential 9 Filter amplifier 9 bandwidth neoane 9 frequency band sss 9 ETT E A a a aaa 9 First functional test 12 G General Performance Noise Level 12 Ground 11 Grounding 12 Grounding the bath 12 H Hub 11 L Lemo connector 11 M Macrolon Quadrate 10 34 MC_Rack software 5 11 MEA Cleaning 10 Microscope 1 9 12 N Noise Level 12 P Pin Layout 30 Power In 11 POWER LED 11 R Rear Panel 20 S Sampling rate 1 9 12 27 Service and Maintenance 12 Stimulation 20 T TCX 9 Technical Specifications 27 Technical Support 27 Temperature Control 1 12 Test model probe 12 30 Toggle Switch I O 11 Triggering other divices 1 9 Troubleshooting 23 27 MEA is defective susss 23 Missing Spikes mano ana rana rana 23 No Computer Connection 23 Noise on Single Electrodes 23 Triggering does not work 23 TTL 1 12 27 43 USB MEA256 System Manual U USB MEA256 System 1 11 Using Electrodes for Stimulation 12 USB 1 11 27 Pin Layout za ei ac coaie dada 12 USB hub 11 Sticker 12 45
8. Connect the internal heating element to a temperature controller s output channel D Sub9 socket with the integrated cable Do not connect the black heating element cable to the computer USB MEA256 System Manual If necessary you can use a Faraday cage or appropriate materials for example aluminum foil for shielding the amplifier The shielding should be connected to the amplifier s ground MEA256 amplifier Please be careful when setting up your perfusion system and when starting the perfusion Take care that the flow rates of the inlet and outlet flow match so that flooding of the amplifier is efficiently prevented Warning Spilled liquid can damage or even completely destroy the electronics of the 4 3 256MEA The microelectrode array 256MEA to be used with the USB MEA256 System contain 252 recording and four ground electrodes on a glass carrier Contact to the amplifier is provided by a double ring of contact pads around the rim of the MEA The electrodes are from Titanium Nitride TiN with a Silicon Nitride SiN isolator and contact pads and tracks are made of transparent Indium Tin Oxide ITO The electrode grid is 16 x16 with a spacing of 30 60 100 or 200 um between the electrodes with a diameter of 8 10 and 30 um The electrode diameter of 30 um results in an impedance of approximately 30 50 kQ Smaller electrodes have a higher impedance so the electrode diameter of 10 or 8 um results in an impedance of approximately 2
9. D1 12 137 254 L7 11 40 42 E13 53 94 182 A3 62 252 195 D2 9 6 224 L8 31 50 35 E14 54 218 243 A4 55 128 159 D3 10 136 194 L9 36 179 5 E15 51 93 179 A5 52 247 131 E1 15 9 55 M10 46 184 69 E16 52 217 147 A6 45 123 229 E2 16 139 25 M11 49 59 100 F11 59 97 183 A7 42 242 202 E3 13 8 123 M5 8 165 14 F12 49 92 180 A8 35 118 166 E4 14 138 93 M6 18 170 78 F13 50 216 145 A9 32 237 138 E5 8 135 253 M7 21 45 105 F14 47 91 177 B10 26 234 206 F1 22 142 91 M8 28 175 7 F15 48 215 191 B11 19 110 170 F2 19 11 56 M9 39 54 33 F16 45 90 178 B12 16 229 144 F3 20 141 26 N10 43 56 34 G11 56 219 245 B13 9 105 242 F4 17 10 124 N11 50 186 70 G12 46 214 192 B14 6 224 211 F5 18 140 94 N12 53 61 97 G13 43 89 175 B15 1 101 240 G1 25 14 122 N13 63 66 27 G14 44 213 189 B16 2 100 210 G2 26 144 92 N5 14 168 77 G15 41 88 176 B3 61 131 225 G3 23 13 53 N6 17 43 108 G16 42 212 190 B4 58 250 198 G4 24 143 23 N7 24 173 10 H10 33 84 150 B5 51 126 162 G5 21 12 121 N8 27 48 38 H11 36 209 185 B6 48 245 134 G6 11 7 156 N9 40 181 6 H12 39 87 153 B7 41 121 232 G7 1 5 155 010 44 183 3 H13 40 211 187 B8 38 240 201 H1 32 147 21 011 47 58 31 H14 37 86 152 B9 29 115 233 H2 29 16 119 012 54 188 67 H15 38 210 188 C10 23 112 167 H3 30 146 89 013 57 63 98 H16 35 85 154 C11 20 231 139 H4 27 15 54 03 3 36 44 H9 60 221 215 C12 13 107 237 H5 28 145 24 04 10 166 80 110 34 208 186 C13 10 226 212 H6 31 17 51 05 13 41 107 111 31 83 151 C14 3 102 172 H7 34 148 90 06 20 171 9 112 28 205 59 C4 57 129 228 11 35 19 52
10. No 13 G45 Conn 4 No 16 H45 Conn 3 No 44 J45 Conn 3 No 16 G51 Conn 4 No 3 H51 Conn 3 No 31 351 Conn 3 No 3 G52 Conn 4 No 6 H52 Conn 3 No 34 J52 Conn 3 No 6 G53 Conn 4 No 7 H53 Conn 3 No 37 353 Conn 3 No 9 G54 Conn 4 No 11 H54 Conn 3 No 39 354 Conn 3 No 11 G55 Conn 4 No 14 H55 Conn 3 No 42 355 Conn 3 No 14 G62 Conn 4 No 5 H62 Conn 3 No 33 J62 Conn 3 No 5 G63 Conn 4 No 10 H63 Conn 3 No 38 J63 Conn 3 No 10 G64 Conn 4 No 12 H64 Conn 3 No 40 J64 Conn 3 No 12 Gs2 Conn 4 No 8 Hs2 Conn 3 No 36 352 Conn 3 No 7 Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel 256 9wellMEA systems Stim Elect Sprin Hard Stim Elect Spri Hard Stim Elec Spri Hard Stim Elect Spri ard Sock rode g ware Sock rode ng war Sock trod ng war Sock rode ng are et ID Con ID et ID Con eID et eID Con eID et ID Con D 1 A35 2 226 1 C35 35 112 1 J41 68 96 1 G41 101 240 2 E31 1 196 2 ES2 34 82 2 E45 67 66 2 ES1 100 210 3 A25 3 158 3 C25 36 44 3 J
11. OA ER HM 82 63 6 6 62 Gb B EN Fa F2 65 63 61 H H2 ds 9 3 3 5 K2 KD O O C5 WP Ma WH ES 02 NA aD ee amp 83 Be 00 19dmuWM Isia ee sa ca am 0 666606006000060606 ee ee e 6 96600600B0ve03060638 ee 0000000000000009 SS E pe os 2 amp 6 660606000960 0w6060639 7 99 0000000000000000 99 000 ee cece amp amp E 0 1 6 tasa a NARA Le na ee 9800000000000 ee cons 00 co Do ko Gn 6b ei fo Gi 4o 9 Kio Go o o 6b i Mo DA iH HRHAMHAAAHAHHG A GAH AAA ee ait eto mmm m Gh d hm m hh n hm ft moor HAHAHAHA m Gh e p o A HH HM oe ee areata ahem Ou E m Cn du m Ku m mm Ou Pu Gu 00 HH e ds de On EB HAH o amp me m m e se BR do de Ge a FAR Gis de dio m m AA 00 BE is i are ta 90 A15 C14 p14 Ria de nis enD 6i Cis En Gr Oe B Eis 1 ABAD AG HAD 046 dio 1 5 3 to da i Cis da dio Ms n Gs io dfe GD die GND Cte t9 Dis Gi Eta Eto F19 FAS Gi Gta Gib Gio dis din 10 He 44 112 15 19 Cto Lia C2 Ms Wis Wis Nia tz O s Ete Ris The letter digit code is the electrode identifier and refers to the position of the electrode in the 16 x 16 layout grid The layout of the letter digit code for the four connectors of the USB MEA256 amplifier is shown To correlate the pin layout of the connectors please see the table on the next page The MEA is rotationally symmetrical so the orientation does not matter If the orientation is important for your experiments you can use the engraved serial number as marker The seri
12. change Germany www multichannelsystems com without notice Appendix 6 7 Data Sheet 256 9wellMEA 35 256 9wellMEA 256 9wellMEA300 30iR ITO w o 256 9wellMEA300 30iR ITO mq multichannel systems 9 well Microelectrode Array for use 2 with USB MEA256 System E L1 st o o o c c O O QUOI mi CASO Di gt Bn Z J0J98Uu09 LI L II N L m m a m a a im O DOO IO COD OO OO OO OO ILICE TE IEDIDOCICICICIDICI 2 The MEA is not symmetrical and has to be inserted into the amplifier with the writing Xe Connector 3 d MCS on top as shown in the picture beside Technical Specifications 9 Well MEA Temperature compatibility Dimension W x D x H Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes Contact pads for reference electrodes connected to ground 0 125 C 49 mm x 49 mm x 1 mm Glass Indium tin oxide ITO 30 um recording 50 x 200 um stimulation 300 um recording 500 um stimulation Planar Titanium nitride TiN Silicon nitride SiN 30 50kQ 6 x 5 recording 2 stimulation electrodes in each well 252 26 recording electrodes in each
13. equipped with 26 round recording electrodes in a 6 x 5 grid two square electrodes S1 and S2 for recording or stimulation and one big reference electrode For detailed information please read chapter 256 9wellMEA and the data sheet If stimulation electrodes S1 and S2 in all wells are to be stimulated simultaneously it is recommended to use the adapter 256MEA 9well STIM ADPT See also the data sheet 256MEA 9well STIM ADPT in the Appendix Please fix the adapter 256MEA 9well STIM ADPT on the connector sockets on the lid of the USB MEA256 amplifier as shown on the picture above The red connectors 2 mm connect to the stimulation electrodes S1 or S2 in each well respectively The black connectors connect to the ground electrodes in each well Three modes of stimulation are possible Monopolar S1 versus ground Connect the stimulator output to the red connector S1 and the stimulator ground to the black connector S1 Monopolar S2 versus ground Connect the stimulator output to the red connector S2 and the stimulator ground to the black connector S2 Bipolar S1 versus S2 Connect the positive stimulator output to the red connector S1 the negative stimulator output to the red connector S2 and the stimulator ground to the black connector S1 or S2 Please see STG manual for details on stimulation modes 18 USB MEA256 System 4 9 The USB MEA256 Device 4 9 1 Rear Panel O CMmemOnono OS Fr DIGITAL IN OUT LED DO DO AUDI
14. for damage each time the site is changed Damaged power cords should be replaced immediately and may never be reused Check the leads for damage Damaged leads should be replaced immediately and may never be reused Do not try to insert anything sharp or metallic into the vents or the case Liquids may cause short circuits or other damage Always keep the device and the power cords dry Do not handle it with wet hands Requirements for the installation Make sure that the device is not exposed to direct sunlight Do not place anything on top of the device and do not place it on top of another heat producing device so that the air can circulate freely Warning The device must not get in contact with fluids Spilled liquid can damage or even completely destroy the electronics of the amplifier This is eminently important when using a perfusion system Take care that the flow rates of the inlet and outlet flow match so that flooding of the amplifier is efficiently prevented 3 1 3 1 1 Software Installation Software Installation Please check the system requirements before you install the MC_Rack software MCS cannot guarantee that the software works properly if these requirements are not fulfilled Please see the MC_Rack Help or Manual for more information It is recommended that you check the MCS web site for software updates on a regular basis The USB MEA256 System is a plug and play device The driver is automatically installe
15. for the biological sample placed on the 256MEA hanne multi stems 21 5 1 5 2 Troubleshooting Troubleshooting The following hints are provided to solve special problems that have been reported by users Most problems occur seldom and only under specific circumstances Please check the mentioned possible causes carefully when you have any trouble with the product In most cases it is only a minor problem that can be easily avoided or solved If the problem persists please contact your local retailer The highly qualified staff will be glad to help you Please inform your local retailer as well if other problems that are not mentioned in this documentation occur even if you have solved the problem on your own This helps other users and it helps MCS to optimize the instrument and the documentation Please pay attention to the safety and service information in the separate manuals of the related products and in the software help Multi Channel Systems has put all effort into making the product fully stable and reliable but like all high performance products it has to be handled with care No Computer Connection No Recording Possible You cannot establish a connection to the computer The USB MEA256 System channel layout is not available in MC_Rack When loading a previously saved virtual rack file you will get an error message and the simulator will be started automatically You get an error message when starting the
16. part 180 um Base material Glass on ceramic carrier Contact pads and track material Indium tin oxide ITO Electrode diameter 30 um Interelectrode distance centre to centre 200 um Electrode height Planar Electrode type Titanium nitride TiN Isolation type Silicon nitride SiN Electrode impedance 30 50kQ Electrode layout grid 16x 16 Number of recording electrodes 252 Number of reference electrodes 4 internal reference electrodes iR Contact pads for reference electrodes connected to ground 4 MC_Rack Source layout in Data Source Setup Configuration Channel map 16 x 16 cmp MEA perfusion chamber w o Without ring Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm r T Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Multi Channel Systems Fon 49 7121 9 09 25 O O 2014 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change without Germany www multichannelsystems com notice multichannel A281 C2E5D3D1E4 2F5 F3 F164G2H5 H3 H1H7 16 1214 K1K3K5 L2 L4 M1 M3 KG N2 18 O1GND B2 03 C167 D2G6 ES3E1 F4F2G563G1H4 H2 H6 17 11 13 15 K2K4 L1 L3 L5 M2 M4 N1N3 L6 2 N4 GND D4 e2P1 A3 B3 03R2 Ha Fe R3 P3 84 C4 K7 M5 oe 886880880086 88008 ee 8 Ssssssssssssssss SE Be cs ee 0000000000000000 se 22 8 amp 666666660666606660 OC se
17. system and when starting the perfusion Take care that the flow rates of the inlet and outlet flow match so that flooding of the amplifier is efficiently prevented Warning Spilled liquid can damage or even completely destroy the electronics of the Grounding the bath 256MEAs are equipped with 4 internal reference electrodes which will be automatically connected to the systems ground when the amplifier is closed This has the advantage that you can keep the culture chamber closed and sterile for example with MEA MEM semipermeable membranes Service and Maintenance You should clean the contact pins of the amplifier carefully with a soft tissue and alcohol or isopropanol from time to time especially if you have problems with the noise level For handling and cleaning of the MEA please refer to the MEA manual or to MEA Cleaning Quick Guide on the MCS web site www multichannelsystems com Warning Please be very careful when handling the amplifier or cleaning the device A The pins can be damaged easily 16 USB MEA256 System Using 256MEA Electrodes for Stimulation The electrodes of the 256MEAs can also be used for electrical stimulation Around the open area for the 256MEA there are two rows of connection sockets on each side in the lid of the amplifier There is one socket for each electrode and four ground sockets These sockets can be used to connect each electrode to a stimulus generator for example a STG4000 from Multi Cha
18. 07 23 46 37 113 27 81 149 c5 54 248 197 12 38 150 87 08 30 176 73 114 30 206 62 C6 47 124 161 13 37 20 117 09 37 53 101 115 29 82 148 C7 44 243 133 14 40 151 19 P1 2 34 82 116 32 207 61 C8 37 119 231 15 39 21 49 P10 41 55 102 K10 7 71 63 C9 30 236 203 16 36 149 22 P11 48 185 4 K11 11 73 247 D10 24 233 140 17 33 18 120 P12 51 60 32 K12 21 78 250 D11 17 109 238 18 60 161 16 P13 58 190 68 K13 24 203 57 D12 14 228 207 K1 42 152 88 P14 61 65 95 K14 23 79 251 D4 63 132 157 K2 41 22 118 P2 1 35 112 K15 26 204 60 D5 53 127 227 K3 44 153 20 P3 6 164 79 K16 25 80 146 D6 50 246 200 K4 43 23 50 P4 9 39 110 L12 18 200 220 D7 43 122 164 K5 46 154 85 P5 16 169 12 L13 17 76 248 D8 40 241 136 K6 56 159 15 P6 19 44 40 L14 20 201 221 D9 27 114 168 L1 45 24 115 P7 26 174 76 L15 19 77 252 E10 21 111 235 L2 48 155 17 P8 29 49 103 L16 22 202 58 E11 18 230 208 L3 47 25 47 P9 38 180 71 M12 8 195 217 E12 8 225 142 L4 50 156 86 R10 42 182 72 M13 14 198 218 E6 49 125 230 L5 49 26 116 R11 45 57 99 M14 13 74 246 E7 46 244 199 L6 59 31 46 R12 52 187 1 M15 16 199 222 E8 39 120 163 M1 52 157 18 R13 55 62 29 M16 15 75 249 E9 28 235 137 M2 51 27 48 R14 62 192 65 N14 10 196 216 F10 11 106 174 M3 54 158 83 R2 4 163 13 N15 9 72 244 F6 59 130 160 M4 53 28 113 R3 5 37 109 N16 12 197 219 F7 56 249 132 N1 55 29 45 R4 12 167 11 014 3 69 28 F8 36 239 135 N2 58 160 84 R5 15 42 39 015 6 194 214 F9 31 116 165 N3 57 30 114 R6 22 172 15 016 5 70 64 G10 7 104 173 N4 63 33 43 R7 25 47 106 P15 1 68 9
19. 1 16 bit digital in out 1 Digital out DO OUT 1 Digital in DO IN 1 Audio output 4 Additional analog inputs USB Ground Power supply Power supply unit MPU 30 Input voltage Output voltage Max Power Software MC_Rack program Operating system MC_DataTool program Data export Appendix up to 40 kHz 20 5 MB s 16 Bit USB 2 0 High Speed 200 PT 100 with 4 wire connection 68 pin MCS standard connectors MCS high grade cable Lemo connector EPL 00 250 NTN Lemo connector EPL 00 250 NTN Stereo jack 3 5 mm Lemo connector EPL 00 250 NTN USB 2 0 High Speed cable type A Mini B Common jack 4 mm banana plug Barrel connector 0 7 x 2 35 mm 90 264 VAC 47 63 Hz 11 13V 30 W Version 3 7 0 and higher Windows 7 XP or Vista with NTFS English and German versions are supported Version 2 4 5 and higher ASCII txt binary file raw format Warning The device may only be used together with Microelectrode Arrays from Multi Channel Systems MCS GmbH and only for the specified purpose Damage of the device and even fatal injuries can result from improper use Do not open the data acquisition box and do not change hardware configuration as it could lead to improper behavior of the system 29 6 3 USB MEA256 System Manual Pin Layout Analog IN A1 to A4 Please note that the channels 127 128 255 and 256 are not used for recording analog raw data from 256MEA in the USB MEA256 Syst
20. 3 246 200 51 B53 27 48 51 FS1 60 32 51 H23 93 179 51 DS2 126 162 52 BS2 157 18 52 F23 187 1 52 HS1 217 147 52 D53 247 131 53 B54 28 113 53 F22 61 97 53 H22 94 182 53 D54 127 227 54 B64 158 83 54 F12 188 67 54 H12 218 243 54 D64 248 197 55 B44 29 45 55 F32 62 29 55 H32 95 181 55 D44 128 159 56 B55 159 15 56 F21 189 2 56 H21 219 245 56 D55 249 132 57 E41 30 114 57 E63 63 98 57 E35 96 184 57 E13 129 228 58 B45 160 84 58 F31 190 68 58 H31 220 213 58 D45 250 198 59 E51 31 46 59 E64 64 30 59 E25 97 183 59 E12 130 160 60 E42 161 16 60 E53 191 125 60 E34 221 215 60 E23 251 129 61 E62 32 111 61 E55 65 95 61 E14 98 239 61 E21 131 225 62 E52 162 81 62 E54 192 65 62 E24 222 209 62 E22 252 195 63 E43 33 43 63 E44 66 27 63 E33 99 171 63 E32 132 157 GND GND GND GND 256 9wellMEA for use with USB MEA256 System Stim Socket Stimulation socket number in the connectors 1 to 4 Spring Contact Spring contacts in the lid of the amplifier connecting to the contact pads of the 256 9wellMEA Hardware ID Hardware channel ID of MC_Rack hardware channels using the linear layout Electrode ID Electrode ID of the MEA electrode in the 6 x 5 2 layout grid Multi Channel Systems Fon 49 7121 9 09 25 0 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info mul
21. 48 38 27 J32 81 149 27 G32 114 168 28 A55 145 24 28 c55 175 7 28 J21 205 59 28 G21 235 137 29 B35 16 119 29 F41 49 103 29 H41 82 148 29 D35 115 233 30 A45 146 89 30 C45 176 73 30 J31 206 62 30 G31 236 203 31 B25 17 51 31 F51 50 35 31 H51 83 151 31 D25 116 165 32 B34 147 21 32 F42 177 8 32 H42 207 61 32 D34 237 138 33 B14 18 120 33 F62 51 104 33 H62 84 150 33 D14 117 234 34 B24 148 90 34 F52 178 74 34 H52 208 186 34 D24 238 204 35 B33 19 52 35 FS2 52 36 35 H43 85 154 35 DS1 118 166 36 BS1 149 22 36 F43 179 5 36 HS2 209 185 36 D33 239 135 37 B23 20 117 37 F53 53 101 37 H53 86 152 37 D23 119 231 38 B13 150 87 38 F63 180 71 38 H63 210 188 38 D13 240 201 39 B22 21 49 39 F54 54 33 39 H54 87 153 39 D22 120 163 40 B12 151 19 40 F64 181 6 40 H64 211 187 40 D12 241 136 41 B32 22 118 41 F44 55 102 41 H44 88 176 41 D32 121 232 42 B21 152 88 42 F55 182 72 42 H55 212 190 42 D21 242 202 43 B41 23 50 43 F35 56 34 43 H35 89 175 43 D41 122 164 44 B31 153 20 44 F45 183 3 44 H45 213 189 44 D31 243 133 45 B51 24 115 45 F25 57 99 45 H25 90 178 45 D51 123 229 46 B42 154 85 46 F34 184 69 46 H34 214 192 46 D42 244 199 47 B62 25 47 47 F14 58 31 47 H14 91 177 47 D62 124 161 48 B52 155 17 48 F24 185 4 48 H24 215 191 48 D52 245 134 49 B63 26 116 49 F13 59 100 49 H13 92 180 49 D63 125 230 50 B43 156 86 50 F33 186 70 50 H33 216 145 50 D4
22. 50 400 kO The dimension of the glass carrier is 49 x 49 x 1 mm MEAs are stable in a temperature range from 0 125 C For information about handling and cleaning please refer to the MEA manual and or to the MEA Cleaning Quick Guide The Pin Layout is described in chapter Data Sheet 256MEA in the Appendix The 256MEA is rotationally symmetrical so in principle the orientation in the amplifier doesn t matter If the orientation is important for your experiments you can use the engraved serial number as marker The serial number is on the backside of the MEA in the upper right edge In the amplifier the mirrored serial number has to be placed in the upper left edge This way the 256 electrode layout matchs with the MC Rack channel layout 10 4 4 4 5 USB MEA256 System 256 Thin MEA The glass part of 256 Thin MEAs are only 180 um thick ideally suited for high resolution imaging 256ThinMEAs are like standard MEAs but the electrodes are embedded in a very thin and delicate glass substrate on a robust ceramic carrier The thin glass allows the use of oil immersion objectives with a high numerical aperture Like 256MEAs 252 electrodes are arranged in a 16 x 16 layout grid with electrode diameter of 30 um and interelectrode distance of 200 um 256ThinMEA200 30 ITO 256ThinMEAs are heat stabilized and can be autoclaved They can also be coated with different procedures for cell and tissue cultures They should be handled wit
23. 51 69 28 3 G51 102 172 4 A34 133 130 4 C34 163 13 4 J42 193 126 4 G42 223 141 5 A14 4 223 5 C14 37 109 5 J62 70 64 5 G62 103 241 6 A24 134 193 6 C24 164 79 6 J52 194 214 6 G52 224 211 7 AS1 5 155 7 C23 38 41 7 JS2 71 63 7 G53 104 173 8 A33 135 253 8 CS1 165 14 8 J43 195 217 8 GS2 225 142 9 A23 6 224 9 C33 39 110 9 J53 72 244 9 G43 105 242 10 A13 136 194 10 C13 166 80 10 J63 196 216 10 G63 226 212 11 A22 7 156 11 C22 40 42 11 J54 73 247 11 G54 106 174 12 A12 137 254 12 C12 167 11 12 J64 197 219 12 G64 227 143 13 A32 8 123 13 C32 41 107 13 J44 74 246 13 G44 107 237 14 A21 138 93 14 C21 168 77 14 J55 198 218 14 G55 228 207 15 A41 9 55 15 C41 42 39 15 J35 75 249 15 G35 108 169 16 A31 139 25 16 C31 169 12 16 J45 199 222 16 G45 229 144 17 A51 10 124 17 C51 43 108 17 J25 76 248 17 G25 109 238 18 A42 140 94 18 C42 170 78 18 J34 200 220 18 G34 230 208 19 A62 11 56 19 C62 44 40 19 J14 77 252 19 G14 110 170 20 A52 141 26 20 C52 171 9 20 J24 201 221 20 G24 231 139 21 A63 12 121 21 C63 45 105 21 J13 78 250 21 G13 111 235 22 A43 142 91 22 C53 172 75 22 J33 202 58 22 G23 232 205 23 A53 13 53 23 C43 46 37 23 J23 79 251 23 G33 112 167 24 AS2 143 23 24 CS2 173 10 24 JS1 203 57 24 GS1 233 140 25 A54 14 122 25 C54 47 106 25 J22 80 146 25 G22 113 236 26 A64 144 92 26 C64 174 76 26 J12 204 60 26 G12 234 206 27 A44 15 54 27 C44
24. 6 E55 Conn 2 No 61 F55 Conn 2 No 42 D62 Conn 4 No 47 E62 Conn 1 No 61 F62 Conn 2 No 33 D63 Conn 4 No 49 E63 Conn 2 No 57 F63 Conn 2 No 38 D64 Conn 4 No 54 E64 Conn 2 No 59 F64 Conn 2 No 40 Ds2 Conn 4 No 51 ES2 Conn 2 No 2 FS2 Conn 2 No 35 Electrode ID Electrode Stimulation Electrode Stimulation Electrode Stimulation and number aD Socket ID Socket ID Socket E GS1 Conn 4 No 24 HS1 Conn 3 No 52 JS1 Conn 3 No 24 of stimulation 612 ___ Conn 4No 26 H12___ Conn 3 No 54 J12 Conn 3 No 26 G13 Conn 4 No 21 H13 Conn 3 No 49 313 Conn 3 No 21 connector socket G14 Conn 4 No 19 H14 Conn 3 No 47 314 Conn 3 No 19 in well G H and J G21 Conn 4 No 28 H21 Conn 3 No 56 321 Conn 3 No 28 G22 Conn 4 No 25 H22 Conn 3 No 53 J22 Conn 3 No 25 G23 Conn 4 No 22 H23 Conn 3 No 51 323 Conn 3 No 23 G24 Conn 4 No 20 H24 Conn 3 No 48 J24 Conn 3 No 20 G25 Conn 4 No 17 H25 Conn 3 No 45 J25 Conn 3 No 17 G31 Conn 4 No 30 H31 Conn 3 No 58 331 Conn 3 No 30 G32 Conn 4 No 27 H32 Conn 3 No 55 332 Conn 3 No 27 G33 Conn 4 No 23 H33 Conn 3 No 50 J33 Conn 3 No 22 G34 Conn 4 No 18 H34 Conn 3 No 46 J34 Conn 3 No 18 G35 Conn 4 No 15 H35 Conn 3 No 43 J35 Conn 3 No 15 G41 Conn 4 No 1 H41 Conn 3 No 29 J41 Conn 3 No 1 G42 Conn 4 No 4 H42 Conn 3 No 32 J42 Conn 3 No 4 G43 Conn 4 No 9 H43 Conn 3 No 35 J43 Conn 3 No 8 G44 Conn 4 No 13 H44 Conn 3 No 41 J44 Conn 3
25. 6 G8 33 117 234 01 62 162 81 R8 32 177 8 P16 4 193 126 G9 34 238 204 02 61 32 111 R9 35 52 36 R15 2 67 66 H8 60 251 129 GND 64 253 GND 64 254 GND 64 255 GND 64 256 Stim Socket Stimulation socket number in the connectors 1 to 4 Spring Contact Spring contacts in the lid of the amplifier Hardware ID Hardware channel ID of MC_Rack hardware channels using the linear layout Electrode ID Electrode ID of the MEA electrode in the 16 x 16 layout grid Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon 49 712 Fax 49 712 info multichannelsystems com www multichannelsystems com 9 09 25 11 9 09 25 0 O 2014 Multi Channel Systems MCS GmbH Product information is subject to change without notice USB MEA256 System Manual 6 6 Data Sheet 256ThinMEA 34 l multichannel 256ThinMEA200 30iR ITO Ow Connector 1 4 ABCDEFGHIKLMNOPR gt 4 gt y Connector 4 Z 10J98uu09 e Y ABCDEFGH KLMNOPR y E Thin microelectrode array with 16 x 16 layout The electrodes are embedded in a very thin glass substrate on a robust ceramic carrier Contact pads and tracks are made from transparent ITO for high resolution imaging Connector 3 Technical Specifications 256ThinMEA200 30iR ITO Temperature compatibility 0 125 C Dimension W x D x H 49 mm x 49 mm x 1 mm Thickness of the glass
26. 8 L1 45 24 115 P7 26 174 76 L15 19 77 252 E10 21 111 235 L2 48 155 17 P8 29 49 103 L16 22 202 58 E11 18 230 208 L3 47 25 47 P9 38 180 71 M12 8 195 217 E12 8 225 142 L4 50 156 86 R10 42 182 72 M13 14 198 218 E6 49 125 230 L5 49 26 116 R11 45 57 99 M14 13 74 246 E7 46 244 199 L6 59 31 46 R12 52 187 1 M15 16 199 222 E8 39 120 163 M1 52 157 18 R13 55 62 29 M16 15 75 249 E9 28 235 137 M2 51 27 48 R14 62 192 65 N14 10 196 216 F10 11 106 174 M3 54 158 83 R2 4 163 13 N15 9 72 244 F6 59 130 160 M4 53 28 113 R3 5 37 109 N16 12 197 219 F7 56 249 132 N1 55 29 45 R4 12 167 11 014 3 69 28 F8 36 239 135 N2 58 160 84 R5 15 42 39 015 6 194 214 F9 31 116 165 N3 57 30 114 R6 22 172 75 016 5 70 64 G10 7 104 173 N4 63 33 43 R7 25 47 106 P15 1 68 96 G8 33 117 234 01 62 162 81 R8 32 177 8 P16 4 193 126 G9 34 238 204 02 61 32 111 R9 35 52 36 R15 2 67 66 H8 60 251 129 GND 64 253 GND 64 254 GND 64 255 GND 64 256 Stim Socket Stimulation socket number in the connectors 1 to 4 Spring Contact Spring contacts in the lid of the amplifier Hardware ID Hardware channel ID of MC_Rack hardware channels using the linear layout Electrode ID Electrode ID of the MEA electrode in the 16 x 16 layout grid Multi Channel Systems Fon 49 7121 9 09 25 O O 2014 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to
27. D 169 43 123 245 110 az 122 2 mas 121 243 12 ge 242 13 120 241 m Y 119 240 us 48 118 239 116 49 117 238 m 50 116 237 ug 51 115 236 19 52 114 235 180 53 113 234 181 54 112 233 182 55 111 232 183 56 110 231 GND GND 184 57 109 230 185 58 108 229 186 59 107 228 187 60 106 227 188 61 105 226 189 62 104 225 190 63 103 224 191 102 223 192 65 101 222221220219 218217 216 215 214 213 212 211 210 209 208 207 206 205 204 203 202 201 200 199 198 197 196 195194193 g 100 99 98 97 96 95 94 93 92 91 90 89 88 87 86 85 84 83 82 81 80 79 78 77 76 75 74 73 72 71 70 69 68 67 Number of the spring contact in the lid of the amplifier which connect to the contact pads of the 256MEA The spring contacts are counted clockwise starting in the upper left edge 31 6 4 USB MEA256 System Manual Test Model Probe The provided model test probe simulates a 256MEA with a resistor of 220 kQ and a 1 nF capacitor between bath and electrode for all 256 electrodes and can be used for testing USB MEA256 Systems GND bath Pad electrode 32 Appendix 6 5 Data Sheet 256MEA 33 256MEA 256MEA30 8iR ITO 256MEA60 10iR ITO 256MEA 100 30iR ITO 256MEA200 30iR ITO 256 Microelectrode Array for use with USB MEA256 System annaas AEREA AAA AAA multichannel systems o Connector 1 E C YedmuM Isito2 4 ABCDEFGHIKLMNOPR gt ee 1 2 3 4 5 6 7 8 Connector 4 Z 10J98uu09
28. No 5 B14 Conn 1 No 33 c14 Conn 2 No 5 connector socket A21 Conn 1 No 14 B21 Conn 1 No 42 c21 Conn 2 No 14 A22 Conn 1 No 11 B22 Conn 1 No 39 c22 Conn 2 No 11 in well A B and C A23 Conn 1No 9 B23 Conn 1 No 37 C23 __ conn 2No 7 A24 Conn 1 No 6 B24 Conn 1 No 34 c24 Conn 2 No 6 A25 Conn 1 No 3 B25 Conn 1 No 31 c25 Conn 2 No 3 A31 Conn 1 No 16 B31 Conn 1 No 44 c31 Conn 2 No 16 A32 Conn 1 No 13 B32 Conn 1 No 41 c32 Conn 2 No 13 A33 Conn 1No 8 B33 Conn 1 No 35 CS1 Conn 2 No 9 A34 Conn 1 No 4 B34 Conn 1 No 32 c34 Conn 2 No 4 A35 Conn 1 No 1 B35 Conn 1 No 29 c35 Conn 2 No 1 A41 Conn 1 No 15 B41 Conn 1 No 43 c41 Conn 2 No 15 A42 Conn 1 No 18 B42 Conn 1 No 46 ca2 Conn 2 No 18 A43 Conn 1 No 22 B43 Conn 1 No 50 c43 Conn 2 No 23 A44 Conn 1 No 27 B44 Conn 1 No 55 c44 Conn 2 No 27 A45 Conn 1 No 30 B45 Conn 1 No 58 C45 Conn 2 No 30 A51 Conn 1 No 17 B51 Conn 1 No 45 C51 Conn 2 No 17 A52 Conn 1 No 20 B52 Conn 1 No 48 c52 Conn 2 No 20 A53 Conn 1 No 23 B53 Conn 1 No 51 c53 Conn 2 No 22 A54 Conn 1 No 25 B54 Conn 1 No 53 C54 Conn 2 No 25 A55 Conn 1 No 28 B55 Conn 1 No 56 C55 Conn 2 No 28 A62 Conn 1 No 19 Be2 Conn 1 No 47 c62 Conn 2 No 19 A63 Conn 1 No 21 B63 Conn 1 No 49 c63 Conn 2 No 21 A64 Conn 1 No 26 B64 Conn 1 No 54 c64 Conn 2 No 26 AS2 Conn 1 No 24 BS2 Conn 1 No 52 cs2 Conn 2 No 24 Electrode ID Electrode Stimulat
29. O Al A2 A3 A4 USB L POWER 1 0 OUT IN GND IN DIGITAL IN OUT A Digital IN OUT for 16 digital in and output bits is available 68 pin MCS standard connector The Digital IN OUT connection accepts or generates standard TTL signals TTL stands for Transistor Transistor Logic A TTL pulse is defined as a digital signal for communication between two devices A voltage between 0 V and 0 8 V is considered as a logical state of 0 LOW and a voltage between 2 V and 5 V means 1 HIGH The Digital OUT allows generating a digital signal with up to 16 bits and read it out for example by using a Digital IN OUT Extension Di o from Multi Channel Systems MCS GmbH You can utilize this digital signal to control and synchronize other devices with the USB MEA256 System Bit O of the Digital OUT is separated and available as Lemo connector DIG OUT DO So if you need only one bit of the digital signal you don t need the additional signal divider SD16 Please read chapter Pin Layout Digital IN OUT Connector in the Appendix for more information The Digital IN can be used to record additional information from external devices as a 16 bit encoded number The Digital IN is most often used to trigger recordings with a TTL signal from a stimulator The 16 bit digital input channels is a stream of 16 bit values The state of each bit 0 to 15 can be controlled separately the state can be HIGH 1 or LOW 0 Standard TTL signals are accepted as i
30. S NY TUPEU TUDO RIA FREUT JOGO UPU TUBO UNIS AA AAA A pad inet nm id lil dl tata bls nios Sa des leia pls hi BO art y E ad a ls lb Dell Ea eel Ca ect CIRIA IRIS CORE RP Ed A A patei A dol nes dlc uid er cvm RTT AER Uum UR eg gr gr enum Vache aei sibiana ed pithi la an ware ilice an Vie adit c ide acts i dl Less setea Ea iil t co cdd Aa 55A mar ad ct NIRO PO LT ORI LY RUEGOS APROPII REIR PR LAITY DORO gt OER OPTA OUR UE a H _ WAAR vidos p AE AE OR E A TUNE FOEN PROPUN JOGO IPR RF FOTO VEA VAGAS JUNG OPENPR PPP ay ITA PRETO TRI E iia UL patada el et lt trado nl aa itg ph co pictis o a mermar iris ptos ho tt lt dis lr cto lr qaae Fe acte ae RADA WORDS SEG SN NIE PDF DD Pl T Lada illo ile Me is lille cite si sie der o Meine o clic MS Ld oii cb tcn ado rol al cercos Iria cl CARIEREI STS RR Un u aN dU IDIOMA gt JUGAR IRI PEPI POPE BE u aN POPOR ATA TPI Setting up an Experiment Open the lid of the amplifier Place the 256MEA chip inside the USB MEA256 amplifier The pin layout is described in chapter Pin Layout in the Appendix The MEA256 is rotationally symmetrical so in principle the orientation in the amplifier doesn t matter If the orientation is important for your experiments you can use the engraved serial number as marker 15 USB MEA256 System Manual Replace the lid and close it carefully MEA256 amplifier Please be careful when setting up your perfusion
31. ady have a basic understanding of technical and software terms No special skills are required to read this manual If you are using the device for the first time please read the Important Safety Advice before installing the hardware and software where you will find important information about the installation and first steps The device and the software are part of an ongoing developmental process Please understand that the provided documentation is not always up to date The latest information can be found in the Help Check also the MCS web site www multichannelsystems com for downloading up to date manuals and help files 1 2 USB MEA256 System Manual Welcome to the USB MEA256 System The USB MEA256 System is a compact and portable stand alone solution for MEA recordings with integrated amplification data acquisition and analog digital conversion It is a highly flexible system The system acquires data from up to 252 electrode channels four additional analog channels and 16 digital IN OUT channels The digitized electrode data is transmitted to the connected computer via universal serial bus High Speed USB 2 0 Thus it is possible to use any computer as a data acquisition computer also a laptop Data is recorded graphed analyzed and reviewed with the powerful and easy to use MC_Rack program from Multi Channel Systems MCS GmbH You can export the data in standard formats to other programs with the software MC_DataT
32. ailer in this case USB MEA256 System Manual 3 1 2 Recommended BIOS settings Recommended operating system settings The following automatic services of the Windows operating system interfere with the data storage on the hard disk and can lead to severe performance limits in MC_Rack These routines were designed for use on office computers but are not very useful for a data acquisition computer 1 2 Turned off Windows System Restore Windows Indexing Service deselected for all local disks Optimize hard disk when idle automatic disk fragmentation turned off It is not recommended to run any applications in the background when using MC Rack Remove all applications from Autostart folder Be careful when using a Virus Scanner These programs are known to disturb MC_Rack and even data loss may occur When using an USB MEA256 System it is recommended to connect a high performance computer with a separate hard disk for program files and data storage The provided possibility to use up to 252 channels with a sample rate of up to 40 kHz needs high memory capacity Please remove data and defragment the hard disk regularly to ensure optimal performance 3 1 3 Driver Installation The USB MEA256 System is a plug and play device The driver is automatically installed together with the MC_Rack program It is easier to connect the USB MEA256 System first to the computer and then install MC_Rack Software Installation 3 1 4 First Us
33. al for more details on how to define the data source 12 USB MEA256 System 4 8 Setting Up the Amplifier Open the lid of the amplifier 1 i Place the 256MEA test model probe inside Replace the lid and close it carefully First Functional Tests Each MEA amplifier has been thoroughly tested at the factory site before delivery However you may want to perform some tests yourself before you begin your experiment to exclude any damage that might have occurred during transportation or to fulfill your own guidelines for instance Some of the tests will also help you to get to know the basic functions of the hard and software like a short tutorial It will take only a few minutes time and can save you time and trouble in the long run Multi Channel Systems recommends running these tests after the setup of your system before you start your real experiments 13 USB MEA256 System Manual General Performance Noise Level Please use the provided test model probe to test the amplifier immediately after installation It simulates a MEA with a resistor of 220 kQ and a 1 nF capacitor between bath and electrode Use MC_Rack or your custom data acquisition program to record from the test model probe and to check the amplifier Please see Test Model Probe in the Appendix Alternative to the test model probe you can use n 256MEA Signal Generator 256MEA Signal Generator is a convenient tool for USB MEA256 System users Use
34. al number is on the backside of the MEA in the upper right edge In the amplifier the mirrored number has to be placed in the left upper edge This way the 256 electrode layout will match the MC_Rack channel layout Multi Channel Systems Fon 49 7121 9 09 25 O O 2014 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 256MEA multichannel systems Stimulation Connector Socket 1 Stimulation Connector Socket 2 Stimulation Connector Socket 3 Stimulation Connector Socket 4 Electrode Stim Spring Hardware Electrode Stim Spring Hardware Electrode Stim Spring Hardware Electrode Stim Spring Hardware ID Socket Contact ID ID Socket Contact ID ID Socket Contact ID ID Socket Contact ID A2 2 1 196 19 60 191 125 C15 61 98 239 A10 25 113 236 B1 4 133 130 K7 1 38 41 C16 62 222 209 A11 22 232 205 B2 1 2 226 K8 34 178 74 D13 63 99 171 A12 15 108 169 C1 5 4 223 K9 33 51 104 D14 57 96 184 A13 12 227 143 c2 6 134 193 L10 56 189 2 D15 58 220 213 A14 5 103 241 c3 3 3 158 L11 59 64 30 D16 55 95 181 A15 4 223 141
35. ay the software version 27 6 2 USB MEA256 System Manual Technical Specifications The USB MEA256 System is a 252 4 channels amplifier with integrated analog digital board converting analog signals to digital data streams in real time It is intended to directly contact to a 252 electrode Microelectrode Array MEA General characteristics Operating temperature Storage temperature Relative humidity Dimensions L x D x H Weight Amplifier Number of electrode channels Input voltage range Data resolution Bandwidth Gain of analog signal path Cross talk channel to channel Additional analog inputs Number of additional inputs Input voltage range Bandwidth Input impedance DC offset Digital inputs amp outputs Number of digital input channels Digital input signal levels Digital input impedance Number of digital output channels Digital output signal levels 28 10 C to 50 C 0 C to 70 C 10 to 85 non condensing 325 4 mm x 207 7 mm x 25 2 mm 2005 g 252 3 7 mV 16 Bit 1 Hz 5 kHz 1100 typical 0 01 max 0 1 4 4 096 V DC to 15 kHz 1 MQ 1 nF max 2 mV 16 CMOS 3 3 V input 100 ko 16 CMOS 3 3 V output Data converter and USB interface Sampling frequency Maximal data rate Data resolution USB Version Heating element and temperature sensor Heating element impedance Temperature sensor type Rear Panel interface and connectors
36. ck Version 3 6 7 and higher Data export software MC_DataTool Version 2 4 3 and higher USB MEA256 System Manual 6 13 Contact Information Local retailer Please see the list of official MCS distributors on the MCS web site User forum The Multi Channel Systems User Forum provides the opportunity for you to exchange your experience or thoughts with other users worldwide Mailing list If you have subscribed to the mailing list you will be automatically informed about new software releases upcoming events and other news on the product line You can subscribe to the list on the contact form of the MCS web site www multichannelsystems com 40 7 Index 41 USB MEA256 System Manual 42 1 16 bit digital IN OUT 1 9 2 256MEA 9 10 23 30 16x16 grid isaten 10 Electrode diameter 10 Electrode impedance 10 Glass carrier 10 Ground electrodes 10 omm 10 Pin Layout 10 30 Recording electrodes 10 SIN isolator ar 10 Spacing between electrodes 10 Temperature range 10 Tin electrodes ccccccococccocccccnnnnnonons 10 256MEA 9well STIM ADPT 34 256MEA STIM C Con 33 25MEA Signal Generator 12 34 9 9 Wells 10 34 256 9wellMEA 10 12 34 256ThinMEA 10 35 A A D converter 1 9 Amplifier 1 9 11 Filters fetu ei ad pa 9 Signal Amplifikation
37. d and continue with Next The Wizard will automatically find the appropriate USB MEA256 System driver Continue with Next The driver installation is finished Please check the system requirements before you install the MC_Rack software MCS cannot guarantee that the software works properly if these requirements are not fulfilled Please see the MC_Rack help or manual for more information It is recommended that you check the MCS web site for software updates on a regular basis Double click Setup exe on the installation volume The installation assistant will show up and guide you through the installation procedure Follow the instructions of the installation assistant The USB MEA256 System driver and MC_Rack are installed or updated automatically 4 1 4 2 USB MEA256 System The USB MEA256 Data Acquisition Analog input signals are acquired from the data source and digitized by the 256 channel analog digital converter that is integrated into the main unit Recorded signals are converted in real time into digital data streams at sampling rates of up to 40 kHz per channel You will not miss even the fastest biological signals Data is transferred to the computer via a High Speed USB 2 0 port A 16 bit digital TTL input output channel is available You can use the digital TTL inputs for example for synchronizing stimulation and recording or for synchronizing the USB MEA256 System with other systems video tracking
38. d The general rule is that at the lower and upper limit of the frequency band the gain is approximately 70 of the full gain Therefore you should use a bandwidth that is at a safe distance of the signals of interest Outside the pass band the gain decreases with the frequency and finally approaches zero For more information on gain and filters in general please refer to standard literature or contact your local retailer Raw data from up to 252 electrodes of a microelectrode array MEA is amplified by 252 channels of filter amplifiers that are built very small and compact using SMD Surface Mounted Devices technology The small sized MEA amplifier combines the interface to the 256MEA probe with the signal filtering and the amplification of the signal The compact design reduces line pick up and keeps the noise level down The MEA sensor is placed directly into the small sized MEA amplifier When the amplifier is closed the contact pins in the lid of the amplifier are pressed onto the MEA contact pads The very close location of the amplifier to the MEA sensor is very favorable concerning a high signal to noise ratio The amplifier is intended to be used either with inverted or with upright microscopes The MEA256 amplifier is compatible to most standard microscopes The MEA256 amplifier has an integrated heating system for controlling the MEA s temperature The desired temperature can be easily regulated with a temperature controller TCX
39. d together with the MC_Rack program It is easier to connect the USB MEA256 System first to the data acquisition computer and then install MC_Rack Important Please make sure that you have full control over your computer as an administrator Otherwise it is possible that the installed hardware does not work properly System Requirements Software One of the following Windows operating systems is required Windows 7 VISTA or XP English and German versions supported with the NT file system NTFS Other language versions may lead to software errors Hardware Not required for offline analysis or demo mode The data acquisition board USB MEA256 System If no USB MEA256 System is present MC Rack opens in a simulation mode A computer with low performance may lead to performance limits more often therefore MCS recommends an up to date computer optionally with a separate hard disk Please note that there are sometimes hardware incompatibilities of the USB MEA256 System and computer components or that an inappropriate computer power supply may lead to artifact signals Please contact your local retailer for more information on recommended computer hardware Important You need to have installed the latest USB MEA256 System driver to operate the USB MEA256 System which is automatically installed with MC_Rack The installation may be invalid if the USB MEA256 System does not respond Please contact Multi Channel Systems or your local ret
40. des are defective use a new MEA Overall Noise Unsteady Baseline The baseline is unstable signals are jumping or drifting Possible causes Bath electrode is not connected to ground Connect the internal or external bath electrode to one of the ground inputs of the amplifier AgCI bath electrode is not well chlorided Rechloride the electrode or use a new one 50 Hz hum 50 Hz is the frequency of mains power in Europe If the shielding and grounding of the setup is not sufficient electrical signals are picked up from the environment Use a proper shielding For example you can place aluminum foil over the amplifier that is connected to any metal part of the MEA amplifier You can also use special shielding equipment like a Faraday cage 25 5 7 USB MEA256 System Manual Missing Spikes or Strange Signal Behavior MEAs wear out after multiple uses or over a longer time of use for example for long term cultures The insulation layer gets thin over time This is considered a normal behavior Possible causes The insulation layer is too thin As a result the MEA gets the behavior of a low pass filter This means that the signal frequency may be shifted to a lower frequency and spikes are missing Optically control the MEA with a microscope If concentric colored rings Newton rings are visible due to light interference the insulation layer is too thin and you should use a fresh MEA The insulation layer
41. drat for quadrat and 9well CC ring 256 9wellMEA to use it as a culture chamber Please insert a foil between quadrat and ring 49 mm Please insert the 256 9wellMEA in correct orientation into the amplifier If you can not read the MCS use the black reference electrodes as marker Multi Channel Systems Fon 49 7121 9 09 25 O O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel 256 9wellMEA systems E Gd Kb b Kb A 6 A42 Ko AS2 AGA ASB M5 B34 e ve ee es E42 E32 GND 465 B ATA A2 2 Kb KB ASI AB AB III IST S22 Se GA 65 82 IT Oe 035 Es E22 E21 C25 C34 E23 E12 Cra C24 S9 C23 cs ee Cas cts D64 D54 C22 612 ee S D43 Des cai 031 052 D62 651 C42 pos emen D31D41 663 C53 D21D32 cas cs2 012022 54 cea 013023 C44 C55 o0 Fat C45 D24D14 F51 F42 oe F62 F52 631035 FS2 F43 ee FS3 rea ci 622 F54 F64 es1 63 Fa F55 enon 2 F25 F34 094625 F14 F24 oos er ss 655 G44 FSA F23 LII F22 F12 G63 643 es 682653 E63 F31 652 662 E64 E55 642651 E55 E54 ES 641 E446Nb 33 Et E25 E35 H32 H22 429 Hio f 444 GND 624 E34 H31 Ha1 H1 AS Ha5 D ABs AB ds 64 bs HS Hi Azi aa A Example Well A The numbering of MEA electrodes in the 6 x 5 grid per each well follows the standard numbering scheme for square grids fang had fa The firs
42. e of MC Rack A 7 8 It is also not recommended to run any applications in the background when using MC Rack Remove all applications from the Autostart folder Warning The operating system settings of the data acquisition computer were preconfigured by MCS and should not be changed by the user Changing these settings can lead to program instabilities and data loss Installing MC_Rack with the USB MEA256 System connected to the computer The Windows operating system detects a new hardware when the USB MEA256 System is connected to the computer if the program has not been installed beforehand Please make sure the device has power that is the power LED is lighting Simply cancel the Found New Hardware Wizard and proceed with the installation of the MC_Rack program Connecting the USB MEA256 System to a computer with installed MC_Rack If MC_Rack had already been installed on the computer before the hardware was connected the operating system needs to load the driver once After this procedure the USB MEA256 System will be automatically recognized by the operating system Connect the USB MEA256 System to the USB port of the data acquisition computer Please make sure the device has power that is the power LED is lighting Switch the computer on The Found New Hardware Wizard is displayed Choose the option No not this time and continue with Next Choose the option Install the software automatically Recommende
43. e sampling rate so that the pulse is missed in between two data points The USB MEA256 System can only accept TTL signals 5 V TTL level as a digital input stream The TTL pulse needs to be optimized according to the sampling rate Otherwise a detection of the trigger cannot be guaranteed The software settings for the Trigger Detector do not match with the hardware configuration In MC_Rack add a Trigger Detector to your virtual rack and select the Digital Data D1 input stream as the Trigger Check the pin layout of the Digital IN OUT connector and make sure that the same bit input that is connected is selected in the software The standard settings of the Trigger Detector are for using bit 0 Mask all unused bits Select the appropriate logical state generally HIGH for triggering Please see the MC_Rack help or manual for more details Noise on Single Electrodes The noise level on single electrodes is significantly higher than expected or you see artifact signals Possible causes The electrode or the contact pin of the amplifier may be defective To test this do the following Open the amplifier and turn the 256MEA by 90 degrees Close the amplifier again and start the recording If the same electrode in the MEA layout is affected the amplifier s contact is not okay If another electrode is now affected and the previously affected electrode is okay now the 256MEA electrode is not okay but the amplifier is fine OR
44. el are working safely Personnel still undergoing training may only work on the device under the supervision of an experienced person Guarantee and Liability The General conditions of sale and delivery of Multi Channel Systems MCS GmbH always apply The operator will receive these no later than on conclusion of the contract Multi Channel Systems MCS GmbH makes no guarantee as to the accuracy of any and all tests and data generated by the use of the device or the software It is up to the user to use good laboratory practice to establish the validity of his her findings Guarantee and liability claims in the event of injury or material damage are excluded when they are the result of one of the following Improper use of the device Improper installation commissioning operation or maintenance of the device Operating the device when the safety and protective devices are defective and or inoperable Non observance of the instructions in the manual with regard to transport storage installation commissioning operation or maintenance of the device Unauthorized structural alterations to the device Unauthorized modifications to the system settings Inadequate monitoring of device components subject to wear Improperly executed and unauthorized repairs Unauthorized opening of the device or its components Catastrophic events due to the effect of foreign bodies or acts of God A USB MEA256 System Manual Important Safety Advice
45. em A1 to A4 are for additional analog data Analog 1 Channel 127 Analog 2 Channel 128 Analog 3 Channel 255 Analog 4 Channel 256 Digital IN OUT Connector 68 Pin MCS Standard Connector 00600 00000 Pin 1 GNDP power ground Pin 2 GNDS signal ground Pin 3 10 Digital output channels bit O 7 Pin 11 14 GNDS signal ground Pin 15 22 Digital output channels bit 8 15 Pin 23 26 GNDS signal ground Pin 27 34 Digital input channels bit 0 7 Pin 35 38 GNDS signal ground Pin 39 46 Digital input channels bit 8 15 Pin 47 48 GNDS signal ground Pin 49 63 Internal use do not connect Pin 64 66 GNDS signal ground Pin 67 Positive supply voltage output Pin 68 Negative voltage supply output Digital Out DO OUT Bit O of the 16 bit digital output channels Pin 3 Digital In DO IN Bit 0 of the 16 bit digital input channels Pin 27 30 Appendix USB MEA256 System Amplifier Stimulation Connector Sockets 1 to 4 6463 A IKLMNOPF veuaraun st 9 O o c z o O Z 10J98uu09 w 1 Connector 3 2 1 E USB MEA256 System Amplifier Spring Contacts 1 2 3 4 5 6 7 8 9 1011 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 ka 133 134 135 136 137 138 129 140 141 442 143 144 145 146 147 148 149 150 151 152 153 154 155 156 15 158 159 160 161 162 130 252 16 37 129 251 184 38 128 250 165 39 127 249 166 40 126 248 167 4 125 247 168 42 124 246 GND GN
46. er supply in the immediate vicinity of the installation site Place all devices on a stable and dry surface where the air can circulate freely and the devices are not exposed to direct sunlight Set up the computer with installed MC_Rack program Set up the MEA256 amplifier as described in chapter Setting up the Amplifier Connect the USB output connector to a free USB 2 0 port of the data proceeding computer It is not recommended to use an USB hub Connect the USB MEA256 via power supply unit to a power outlet of the same electrical system connected to the same ground earth wire as all other components of the setup for example the computer or shielding If necessary connect the system to an external ground Connect the internal heating element to the temperature controller s output channel D Sub9 socket with the integrated cable Do not connect the heating element cable to the computer Turn the toggle switch at the rear panel on Check the power LED It should light up as soon as the power line is connected and the toggle switch is switched on If not check the power source and cabling Install the MC_Rack program from the installation volume if it is not already installed The USB MEA256 System is a plug and play device The driver is automatically installed together with the MC_Rack program Start the MC_Rack program and select the USB MEA256 System as the data source Please see the MC_Rack help or manu
47. for example The digital TTL outputs can be used for triggering other devices The USB MEA256 Filter Amplification The filter amplifier combines a band pass filter and the signal amplification in one instrument The bandwidth of 1 to 5000 Hz is suitable for a broad range of applications such as spike and field potential recording from neurons or recording of cardiac signals The digital filter of the MC_Rack program can be used to adjust the pass band and filter the raw data Please see the MC_Rack help or manual for more information This way you are very flexible in designing your experiments Please note that you may need a higher sampling rate to avoid aliasing See also the chapter USB MEA256 Data Acquisition for more information For slow signals like field potentials a bandwidth of 1 to 300 Hz is appropriate If you like to record fast signals like spikes a pass band of 300 Hz to 5 kHz is suitable Cardiac signals have fast and slow components therefore you usually need a wider bandwidth of 1 Hz to 5 kHz Please note that the gain factor of the filter amplifier 1100 is a fixed hardware property and that you cannot change the gain of the amplifier by software controls Please also note that the ratio of the output signal to the input signal that is the gain is not a fixed parameter for the complete bandwidth The gain that was specified for the amplifier for example 1100 is not fully reached at the borders of the amplifier s pass ban
48. gain specified in MC_Rack USB The USB connector is used to transfer the amplified and digitized data from all data channels and the additional digital and analog channels to any connected data acquisition computer via High Speed USB 2 0 type A mini B cable GROUND If an additional ground connection is needed you can connect this plug with an external ground using a standard common jack 4 mm POWER IN Connect the power supply unit here This power supply powers the USB MEA256 main unit only The device needs 12 V and 1 3 A 16 W Toggle Switch I O Toggle switch for turning the device on and off The USB MEA256 is switched to status ON when the toggle switch is switched to the left to I The device is switched OFF when the toggle switch is switched to the right O If the USB MEA256 is ON and the device is connected to the power line the LED should light up If not please check the power source and cabling 20 USB MEA256 System Cabling For DO IN and DO OUT and Analog Input A1 to A4 on the rear panel of the device you need coaxial cable Lemo cable with one head BNC connector the other head Lemo connector Please see the picture below Temperature Controller TCO1 02 The USB MEA256 System features a heating element with a PT100 temperature sensor If you connect a temperature controller TCX to the heating element via D Sub 9 connector the heating element guarantees constant temperature conditions
49. h great care because of the thin and delicate recording area Please see chapter Data Sheet 256ThinMEA in the Appendix 256 9well MEA MCS NULL A special MEA layout for the USB MEA256 System is available the 256 9wellMEA for using together with a 9 well macrolon quadrate The 9 wells allow for example drug screening experiments with up to 9 compounds at a time The 256 9wellMEA is in principle constructed like the 256MEA but the electrodes are clustered There are 23 recording electrodes with a diameter of 30 um two bigger recording or stimulation electrodes 2 x 50 um and one big reference electrode per each well Please see chapter Data Sheet 256 9wellMEA in the Appendix 11 4 6 4 7 USB MEA256 System Manual The USB MEA256 System USB MEA256 System is a high flexible system with integrated amplification data acquisition and analog digital conversion Via USB High Speed 2 0 it is possible to transfer a digitally converted and amplified data stream of up to 252 electrode channels to any data acquisition computer Note Using a USB hub for connecting the USB MEA256 System to the computer is not recommended The system needs a broad bandwidth for the data transmission Recording might not be possible especially if a second device that sends or receives continuous data streams for example a web cam or USB speakers is connected to the same USB port Top View Connecting the USB MEA256 System Provide a pow
50. has been abraded and is missing in parts This will result in a short circuit between the electrode tracks and the bath You will still see signals but as an unspecific smear over the complete array Use a fresh MEA 26 6 1 Appendix Technical Support Please read the chapter Troubleshooting of the user manual first Most problems are caused by minor handling errors Contact your local retailer immediately if the cause of trouble remains unclear Please understand that information on your hardware and software configuration is necessary to analyze and finally solve the problem you encounter Please keep information on the following at hand Description of the error the error message text or any other useful information and of the context in which the error occurred Try to remember all steps you had performed immediately before the error occurred The more information on the actual situation you can provide the easier it is to track the problem The serial number of the device You will find it on the backside of the housing or in MC Rack Hardware tab The operating system and service pack number of the connected computer The hardware configuration microprocessor frequency main memory hard disk of the connected computer This information is especially important if you have modified the computer or installed new hard or software recently The version of the recording software On the Help menu click About to displ
51. iment with biological sample for training controlling and troubleshooting purposes This reduces the number of animal experiments and saves laboratory equipment Switch on Press control button Switch off Press control button longer than two seconds Important To change DIP switch position please switch off the device Table DIP switch positions number of control button presses and corresponding signals Switch 1 Switch 2 Control button Signal presses n times OFF OFF MEA SG ON Sinus 0 005 Hz 1 Sinus 0 01 Hz 2 Sinus 0 03 Hz 3 Sinus 1 25 Hz 4 Sinus 12 5 Hz ON OFF MEA SG ON EPSP L 1 Population Spike 2 Spikes OFF ON MEA SG ON ECG Atrium ot 1 ECG Ventricle 2 Ventricle FP ON ON MEA SG ON ERG with Spikes Multi Channel Systems Fon 49 7121 9 09 25 0 O 2014 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 256MEA SG 256MEA Signal Generator multichannel systems Switch press Signal Source Signal Type 1 Button n times 1 Artificial Sine Wave 1 75 mV 0 005 Hz 256MEA SG Note sine waves lt 1Hz might not be ON visible because of the hardware filter bandwidth 3 Sine Wave 4 Sine Wave 5 Sine Wave ON OFF 1 Hippocampal Slice 256MEA SG EPSP ON 1 Hippocampal Slice Pop
52. ion Stimulation Electrode Stimulation and number aD Soc ID Socket ID Socket i i DS1 Conn 4 No 35 ES1 Conn 4 No 2 FS1 Conn 2 No 51 of sti mulation D12 Conn 4 No 40 E12 Conn 4 No 59 F12 Conn 2 No 54 connector socket D13 Conn 4 No 38 E13 Conn 4 No 57 F13 Conn 2 No 49 D14 Conn 4 No 33 E14 Conn 3 No 61 F14 Conn 2 No 47 in well D E and F D21___ Conn 4 No 42 E21 Conn 4 No 61 F21 Conn 2 No 56 D22 Conn 4 No 39 E22 Conn 4 No 62 F22 Conn 2 No 53 D23 Conn 4 No 37 E23 Conn 4 No 60 F23 Conn 2 No 52 D24 Conn 4 No 34 E24 Conn 3 No 62 F24 Conn 2 No 48 D25 Conn 4 No 31 E25 Conn 3 No 59 F25 Conn 2 No 45 D31 Conn 4 No 44 E31 Conn 1 No 2 F31 Conn 2 No 58 D32 Conn 4 No 41 E32 Conn 4 No 63 F32 Conn 2 No 55 D33 Conn 4 No 36 E33 Conn 3 No 63 F33 Conn 2 No 50 D34 Conn 4 No 32 E34 Conn 3 No 60 F34 Conn 2 No 46 D35 Conn 4 No 29 E35 Conn 3 No 57 F35 Conn 2 No 43 D41 Conn 4 No 43 E41 Conn 1 No 57 F41 Conn 2 No 29 D42 Conn 4 No 46 E42 Conn 1 No 60 F42 Conn 2 No 32 D43 Conn 4 No 50 E43 Conn 1 No 63 F43 Conn 2 No 36 D44 Conn 4 No 55 E44 Conn 2 No 63 F44 Conn 2 No 41 D45 Conn 4 No 58 E45 Conn 3 No 2 F45 Conn 2 No 44 D51 Conn 4 No 45 E51 Conn 1 No 59 F51 Conn 2 No 31 D52 Conn 4 No 48 E52 Conn 1 No 62 F52 Conn 2 No 34 D53 Conn 4 No 52 E53 Conn 2 No 60 F53 Conn 2 No 37 D54 Conn 4 No 53 E54 Conn 2 No 62 F54 Conn 2 No 39 D55 Conn 4 No 5
53. multichannel systems USB MEA256 System Manual Information in this document is subject to change without notice No part of this document may be reproduced or transmitted without the express written permission of Multi Channel Systems MCS GmbH While every precaution has been taken in the preparation of this document the publisher and the author assume no responsibility for errors or omissions or for damages resulting from the use of information contained in this document or from the use of programs and source code that may accompany it In no event shall the publisher and the author be liable for any loss of profit or any other commercial damage caused or alleged to have been caused directly or indirectly by this document O 2014 Multi Channel Systems MCS GmbH All rights reserved Printed 17 04 2014 Multi Channel Systems MCS GmbH Aspenhaustra e 21 72770 Reutlingen Germany Fon 49 71 21 90 925 0 Fax 49 71 21 90 92 5 11 info multichannelsystems com www multichannelsystems com Microsoft and Windows are registered trademarks of Microsoft Corporation Products that are referred to in this document may be either trademarks and or registered trademarks of their respective holders and should be noted as such The publisher and the author make no claim to these trademark 1 1 1 2 2 1 2 2 2 3 4 1 4 2 4 3 4 4 4 5 4 6 4 7 4 8 4 9 5 1 5 2 5 3 5 4 5 5 5 6 5 7 Table of Contents Introduction Abo
54. nally one big internal reference electrode Please fix the adapter 256MEA 9well STIM ADPT on the USB MEA256 amplifier as shown on the foto Connect the red connectors 2 mm with the provided cables to the STG for stimulation of S1 and S2 The black connectors 2 mm are for grounding the system Stimulation of S1 means that the S1 electrodes in each of the nine wells are stimulated altogether Stimulation of S2 means that the S2 electrodes in each of the nine wells are stimulated altogether It is not possible to stimulate only one of the nine stimulation electrodes S1 or S2 for example Warning The 256MEA 9well STIM ADPT may only be used together with the A USB MEA256 System from Multi Channel Systems MCS GmbH and only for the specified purpose Damage of the device and even injuries can result from improper use Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fax 49 7121 9 09 25 11 Fon 49 7121 9 09 25 O O 2013 Multi Channel Systems MCS GmbH info multichannelsystems com Product information is subject to change www multichannelsystems com without notice USB MEA256 System Manual 6 10 256MEA Signal Generator 38 multichannel systems 256MEA SG 256MEA Signal Generator for use with USB MEA256 System Control button LED DIP switch Battery Gold contact pads 256MEA Signal Generator is a convenient tool for USB MEA256 System users Use the 256MEA SG instead of setting up an exper
55. nnel Systems MCS GmbH The ground sockets can be used to connect other devices like the stimulator or the perfusion to the systems ground You will find the layout map of the sockets in chapter Pin Layout in the Appendix To make it easier to find the correct socket for each electrode stickers are included to color code the electrode sockets in four blocks of 2 x 8 sockets corresponding to the color code used in the layout map in chapter Pin Layout in the Appendix If you want to do electrical stimulation please attach the stickers as shown in the image below The edge of the sticker has to be aligned with the first pair of sockets The position of the four ground sockets is labeled with a G The connection sockets are arranged in quadrants The electrodes in quadrant 1 have their sockets in the upper row electrodes in quadrant 2 in the right row and so on To find the connection socket for a specific electrode please do following Determine the quadrant the electrode is in Look up the exact position of the socket in the layout map Determine the label yellow grey the socket belongs to Count the sockets from the edge of the label Plug in the stimulation adapter ADPT STIM MEA256 in the correct connector socket Connect the ADPT STIM MEA256 via laboratory cable with the stimulus generator STG 17 USB MEA256 System Manual Using 256 9wellMEA Electrodes for Stimulation Each well of the nine wells of the 256 9wellMEA is
56. nput signals on the digital inputs Unused input bits which have an undefined state should be masked in the Trigger Detector of MC Rack voltage applied to the digital input would destroy the electronics Make sure that you apply Warning A voltage that is higher than 5 Volts or lower than 0 Volts that is a negative only TTL pulses 0 5 V to the digital inputs Power LED The Power LED lights up when the USB MEA256 is connected via power supply unit to the power supply system and the toggle switch on the rear panel is switched on Digital Output DO OUT The Bit O of the Digital OUT is also accessible independently from the 68 pin Digital IN OUT connector with a Lemo connector The digital output channel DO is generally used for synchronizing the USB MEA256 System with a stimulus generator or with another data acquisition system for example an imaging or a patch clamp system The DO OUT generates standard TTL pulses Digital Input DO IN The Bit O of the Digital IN is also accessible independently from the 68 pin Digital IN OUT connector with a Lemo connector The digital output channel DO is generally used for synchronizing the USB MEA256 System with a stimulus generator or with another data acquisition system for example an imaging or a patch clamp system The DO IN accepts standard TTL pulses 19 USB MEA256 System Manual Audio Output AUDIO To the AUDIO OUT 3 5 mm phone jack you can connect an audio system t
57. o make the electrical activity audible In contrast to the sound tool of MC_Rack this audio output is real time There is almost no time delay between the detection of a signal by the recording electrode and the corresponding sound Headphones or a speaker can be connected directly to the AUDIO OUT Only one channel can be converted into sound This channel can still be selected by using the sound instrument of MC_Rack You can listen only to Electrode Raw Data in real time The quality of the sound is mono Note You can add only one sound instrument to your rack and you can convert only one channel into sound Analog Input A1 to A4 Four additional analog inputs are available Lemo connectors The amplifier delivers analog data streams from 252 channels to the integrated data acquisition and A D converter of the USB MEA256 The remaining four channels 127 128 255 and 256 are used for the additional analog inputs Analog IN A1 to A4 The additional analog inputs one to four are intended for recording additional information from external devices for example for recording patch clamp in parallel to the MEA recording for monitoring the temperature or for recording voice You could also use the analog inputs for triggering but please note that the digital inputs especially DO IN are intended for accepting TTL pulses Signals on the analog channels are not amplified They are only digitized and recorded as they are with no respect to the
58. onizing the USB MEA256 System with other systems The digital TTL outputs can be used for triggering other devices for example an imaging setup The 16 bit Digital IN OUT channel can be contacted with a 68 pin MCS standard connector The bits O from the D IN and the D OUT respectively are also accessible independently by the DO IN and DO OUT Lemo connectors The additional analog inputs 1 to 4 are intended for recording additional information from external devices for example for recording patch clamp in parallel to the MEA recording for monitoring the temperature or for recording voice To the Audio Out 3 5 mm phone jack you can connect an audio system to make the electrical activity audible This audio output is real time Headphones or a speaker can be connected directly to the AUDIO OUT Only one channel at a time can be converted into sound 2 2 1 2 2 Important Safety Advice Operator s Obligations The operator is obliged to allow only persons to work on the device who are familiar with the safety at work and accident prevention regulations and have been instructed how to use the device are professionally qualified or have specialist knowledge and training and have received instruction in the use of the device have read and understood the chapter on safety and the warning instructions in this manual and confirmed this with their signature It must be monitored at regular intervals that the operating personn
59. ool Instead of MC_Rack any other common data processing program or custom software can be used The integrated filter amplifier supports 252 electrode channels The amplification factor of 1100 is fixed The bandwidth of 1 Hz to 5 kHz is suitable for a broad range of applications such as spike and field potential recording from neurons or recording of cardiac signals Electrode raw data are acquired from the 256MEA and digitized by the analog digital converter board that is integrated into the main unit Recorded signals are converted in real time into digital data streams at sampling rates of up to 40 kHz per channel You will not miss even fast biological signals Data is transferred to the computer via High Speed USB 2 0 port The USB MEA256 System features a heating element with a PT100 temperature sensor If you connect a temperature controller TCX to the heating element via D Sub 9 connector the heating element guarantees constant temperature conditions for the biological sample placed on the 256MEA Microelectrode Array with 252 recording electrodes and four reference electrodes or on the 256ThinMEA or the 256 9wellMEA respectively To control the biological sample on the 256MEA inside the amplifier optically you can use either an upright microscope or an inverted microscope A 16 bit digital input output TTL is available You can use the digital TTL inputs for example for synchronizing stimulation and recording or for synchr
60. recording in MC_Rack after a successful computer connection Possible causes The power LED is not lightning The supply power is not connected or there is a technical problem with the instrument Check the power source and the cable connections If this does not solve the problem contact your local retailer for support The power LED is lighting The USB port might not support USB 2 0 or might not be working Check the USB port Only full speed USB 2 0 ports can be used Try another USB 2 0 port You can establish a connection but get an error message when starting the recording in MC_Rack The bandwidth of the USB port is not sufficient for recording This can be the case if the USB MEA256 System is connected via USB hub and a second device that sends or receives continuous data streams for example a web cam or USB speakers is connected to the same USB port Connect the USB MEA256 System directly to a USB port not via hub 23 5 3 5 4 USB MEA256 System Manual Triggering Digital Input does not Work You have connected a TTL source for example the Sync Out of a stimulus generator to the digital input DO of the USB MEA256 System and configured the virtual rack in MC_Rack for triggering displays or data acquisition by the TTL source but you do not see any sweeps Possible causes The TTL source does not generate true TTL signals 5 V TTL level or the TTL pulse duration is too short in combination with th
61. rrect socket for each electrode stickers are included to color code the electrode sockets in four blocks of 2 x 8 sockets corresponding to the color code used in the layout map in chapter Pin Layout If you want to do electrical stimulation please attach the stickers as shown in the image above The edge of the sticker has to be aligned with the first pair of sockets Multi Channel Systems Fon 49 7121 9 09 25 0 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice Appendix 6 9 Data Sheet 256MEA 9well STIM ADPT 37 multichannel systems 256MEA 9well STIM ADPT Adapter for Electrode Stimulation of 9 Well MEAs in USB MEA256 Systems Front panel of the adapter 256MEA 9well STIM ADPT The red connectors are for stimulation of the electrodes S1 and or S2 in each well The black connectors are for grounding the system eese A eeeece 006066 TE Uum 0886 Example Well A The numbering of MEA electrodes in the 6 x 5 grid per each well follows the standard numbering scheme for square grids The first digit is the column number and the second digit is the row number For example electrode 23 is positioned in the second column of the third row Two square electrodes S1 and S2 per well are available for stimulation or recording and additio
62. t digit is the column number and the second digit is the row number For example electrode 23 has 33 is positioned in the second column of the third row Two square electrodes S1 and S2 per well are available for stimulation or recording There is one big internal reference electrode in each 135 J44 J54 153 US2 d62 451 Jan is Has das ea a 59 dis dia d51 Han E JAS J55 J64 63 J43 J52 J42 E45 has bas well The nine reference electrodes are connected to four contact pads for grounding them Multi Channel Systems Fon 49 7121 9 09 25 O O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel 256 9wellMEA systems Tables Stimulation Electrode ID 1D Socket 1D os i AS1 Conn 1 No 7 BS1 Conn 1 No 36 cst Conn 2 No 8 and number A12 Conn 1 No 12 B12 Conn 1No 40 C12 Conn 2 No 12 i i A13 Conn 1 No 10 B13 Conn 1 No 38 C13 Conn 2 No 10 of sti mulation A14 Conn 1
63. the 256MEA SG instead of setting Up an experiment with biological sample for training controlling and troubleshooting purposes This reduces the number of animal experiments and saves laboratory equipment Please see the data sheet 256MEA SG in the Appendix Setting up MC_Rack Please refer to the MC_Rack manual for more information Start MC_Rack Click Data Source Setup on the Edit menu Select USB MEA256 Add USB MEA256 as a data source to your virtual rack On the Edit menu click Add Data Display to add a raw data display to your virtual rack Click the Hardware tab of the USB MEA256 and enter the sampling frequency in Hz FF Rack1 Rack Data Source USB MEA256 S N 00056 10l x E Recorder BB uss meazse sjM 00056 Rack Hardware Info Audio fred Display 1 Settings Amplifier Gain 1100 00 Enter Input Voltage Range of 4036 0 to 4096 0 mV gt Data Acquisition Board 4036 0 to 4096 0 mV Sampling Frequency 25000 Y Hz Driver Version 3 6 Device USB MEA256 S N xxxxx Fw 2 02 1 29 Hw 32a Number of Channels Analog 255 Digital 1 ADC Resolution 16 bit Data Format 16 bit Offset Correction Hardware Info Learn Offsets Offset Correction Refresh Start the data acquisition Click the Start button to start the data acquisition No data is recorded You see the raw data streams of all 252 channels in the 16 x 16 MEA layout Yo
64. tichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice USB MEA256 System Manual 6 8 Data Sheet 256MEA STIM C Con 36 multichannel 256MEA STIM C Con systems Connector for Electrode Stimulation of 256MEAs in USB MEA256 Systems i Connector for inserting the stimulation cable CB and or CR from the STG into the provided stimulation sockets of the USB MEA256 System Stimulation cable CB black and CR red with connected adapter The stimulation cables have a 0 76 mm connector on the head for the adapter and a 1 0 mm connector on the head for the stimulus generator USB MEA256 System top view with signal generator 256MEA SG and stimulation cable CR red for stimulation of a electrode and cable CB black for grounding the system The electrodes of the 256MEA can also be used for electrical stimulation Around the 256MEA there are two rows of connection sockets on each side There is one socket for each electrode and four ground sockets These sockets can be used to connect each electrode to a STG for example a stimulus generator of STG4000 series from Multi Channel Systems MCS GmbH The ground sockets can be used to connect other devices like the stimulator or the perfusion to the systems ground You will find the layout map of the sockets in chapter Pin Layout in the Appendix of the USB MEA256 System manual To make it easier to find the co
65. u may have to adjust the position and span of the axes until you can clearly see the noise level You should see the baseline with a maximum noise level of 8 uV 14 USB MEA256 System The following screen shot shows a recording from a USB MEA256 System with a test model probe and a sampling rate of 40 kHz p MC_Rack Display 1 Fie Edit Measurement View Window Help S r m A Display 1 420 600 sec XAxs 1000 ms gt 00 10000 I Peak Detection T Reverse Display al 412th sweep z N xis 1 gt uv ar gt 10 WF Showlmage 0 sweeps skipped rs rr a rd rn rt rr PERU POP A OTA abit de eto di ida de Lid diri fo dal ial ir cli O sites it esuata bl Mertens canis llei laos Me Ed INEA R iE LS lt a APRA INR EPIRI QUIET lt P IPUR PP coate ate sait cde bolts Lih dt tenta ui se tcl schien chen Priza absit e Rhe hcl Mna o tei vl es DR PR OR RER EA RR NR PRR AP manat RR ital aca xt tit la tac ii cni MD NAR Mama a NM a te a a elt i A A A A OA ee a s su Po Itaca bc sb lids hc vol ade raa pi cota ti os Natt u o le E atte lr ae e ini a lr ltd terio Pt de A IIA CHEER PRO UNO REPERTA PEPI PPE DEPOT PAPINI REUS VACARE SUE en PU tenace Vital Minas inea ciini per cb IA Bica Ata sl anca CO soli pasan est ibat usn Ll al kata lea A CRI rmm 5 ee AT A at pana Enea WERE OS iara avast ehe ac acea Pt ERBAUT IP cu catia ic att acted ii Lose tici ina E Sehen N AO RD ZORII eee PD CTETUR T
66. ulation Spike 2 Hippocampal Neurons Spikes OFF ON 1 Heart 256MEA SG ECG Atrium ON 1 Heart ECG Ventricle 2 Cardiomyocytes Ventricle FP ON ON 1 Retina 256MEA SG ERG with Spike ON Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 O 2014 Multi Channel Systems MCS GmbH info multichannelsystems com www multichannelsystems com Product information is subject to change without notice Appendix 6 11 Digital IN OUT Extension Input bits 0 to 15 Output bits 0 to 15 8 9 10 11 8 9 10 11 iS 14 15 12 13 14 15 16 bit Digital IN OUT Connector 68 Pin MCS Standard Connector 68 Pin MCS Standard Connector 0 9 2 2 OOOO Pin 1 GNDP power ground Pin 2 GNDS signal ground Pin 3 10 Digital Output channels bit 0 to 7 Pin 11 14 GNDS signals ground Pin 15 22 Digital output channels bit 8 to 15 Pin 23 26 GNDS signals ground Pin 27 34 Digital input channels bit 0 to 7 Pin 35 38 GNDS signals ground Pin 39 46 Digital output channels bit 8 to 15 Pin 47 48 GNDS signals ground Pin 49 63 Internal use please do not connect Pin 64 66 GNDS signals ground Pin 67 Positive supply voltage Pin 68 Negative supply voltage 39 USB MEA256 System Manual 6 12 Scope of Delivery LI M RI BILE II ooo AER a d Power supply unit with country AA UNU el power cord anim Data acquisition and analysis software MC_Ra
67. ut this Manual Welcome to the USB MEA256 System Important Safety Advice Operator s Obligations Guarantee and Liability Important Safety Advice Software Installation Software Installation 3 1 1 System Requirements 3 1 2 Recommended BIOS settings 3 1 3 Driver Installation 3 1 4 First Use of MC Rack USB MEA256 System The USB MEA256 Data Acquisition The USB MEA256 Filter Amplification 256MEA 256 Thin MEA 256 9well MEA The USB MEA256 System Connecting the USB MEA256 System Setting Up the Amplifier The USB MEA256 Device 4 9 1 Rear Panel Troubleshooting Troubleshooting No Computer Connection No Recording Possible Triggering Digital Input does not Work Noise on Single Electrodes MEA is defective Overall Noise Unsteady Baseline Missing Spikes or Strange Signal Behavior N PP ww y N O O U U UI 10 11 11 12 12 13 19 19 23 23 23 24 24 25 25 26 USB MEA256 System Manual 6 1 6 2 6 3 6 4 6 5 6 6 6 7 6 8 6 9 6 10 6 11 6 12 6 13 Appendix Technical Support Technical Specifications Pin Layout Test Model Probe Data Sheet 256MEA Data Sheet 256ThinMEA Data Sheet 256MEA STIM C Con Data Sheet 256 9wellMEA Data Sheet 256MEA 9well STIM ADPT 256MEA Signal Generator Digital IN OUT Extension Scope of Delivery Contact Information Index 27 27 28 30 32 33 34 36 35 37 38 39 40 40 41 1 1 Introduction About this Manual It is assumed that you alre
68. well 9 1 internal reference electrode iR in each well 4 Source layout in Data Source Setup Configuration Channel map 9 well 256MEA cmp MEA perfusion chamber w o Without Macrolon quadrat mq Macrolon quadrat with 9 wells ID 6 5 x 6 5 mm of each well OD 24 x 24 mm of all wells height 9 mm Volumetric capacity of each well minimum 250 ul Multi Channel Systems Fon 49 7121 9 09 25 0 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel 256 9wellMEA systems Macrolon Quadrat MCS 1 2 mm 0 8 mm 0 8 mm 1 2 mm OOOO Oooo Slot to insert A A pA an O ring for T co e e Li membrane Ed DH 3 O B covering Ln EI EH oH EH EI rd Urn m EI Us i pd EH mm gH Us 3 En On En EI EL OF H LI d OF O y b OF oH TY Y F o E 3 DH Us ON B Ea pH um f OH ad A m A O On A 1 i pH e e E T E ED m mii Cno O 9 Well macrolon 256 9wellMEA with macrolon qua

USB-MEA256-System Manual

Contents

Download Pdf Manuals

Related Search

Related Contents