1100 SERIES - Masterflex
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1. If the energy is low adjust the lamp by pulling or pushing it Since the lamp socket is pre aligned there will be minimum if any adjustment required 6 Re install the grill plate Wavelength Calibration Normally the Cole Parmer 1100 Series spectrophotometer retains its wavelength calibration indefinitely However if the instrument receives a severe shock or is abused use the following methods to check wavelength calibration Please note that this test requires the Cole Parmer Didymium filter p n 1100 110 or the Holmium Oxide filter p n 1100 109 In the filter method the didymium filter has two distinct absorbance peaks at 529nm and 807nm The Holmium filter has a distinct peak at 361nm When the instrument is calibrated properly you will find minimum Transmittance maximum Absorbance at the range 2nm from these peaks Note that the specific Transmittance values are not important as you are only looking for the wavelength where the minimum transmittance maximum Absorbance occurs Holmium Oxide Filter Method 1 Turn instrument on and allow it to warm up for 15 minutes 12 Select the Absorbance operating mode Set the wavelength to 350nm Make sure the cuvette holder is empty and place it in the sample compartment Close the sample compartment lid Set zero Absorbance by pressing the 0A 100 T Wait a few seconds while the display flashes BLA The reading should then be 0 0004 If not repeat step 5 Remove2. Mode for determining the concentration of unknown samples NOTE This method should only be used when the relationship between Absorbance and Concentration is known to be linear The concentration of the Standard solution used to calibrate the instrument should be higher than the most concentrated sample pudo ect 7 Select the desired wavelength by turning the wavelength control knob Using the MODE button select Absorbance A mode Insert the cuvette containing the blank solution Set 0 000A with the 0A 100 T control button Using the MODE button select C concentration mode Insert a cuvette containing a standard solution of known concentration in the sample compartment and adjust the digital readout window to the value of the standard by using the and v arrows Press the Enter button NOTE If the reading changes the factor required is too high i e gt 1999 to be 8 displayed In this case divide the concentration by 10 re select the C mode by successive presses on the Mode button cycling through the F 6T and A modes and follow step 2 above to set the concentration of the standard to the reduced value 8 With the standard concentration set determine the concentration values of samples with unknown concentration by inserting the sample cuvette into the sample compartment and reading the value direct from the display 9 To read the value of the multiplier used to convert Abs to Concentration after meas
3. 0 000A using the 0A 100 T button T Empty the cell Wash out with dichromate solution and fill with dichromate solution 14 8 Put the cuvette in the adapter into the sample compartment and close the lid 9 Read the absorbance of the standard from the display The value should be 1 288 0 04A Refer to the notes above when interpreting the result Stray Light Check Specification Less than 0 5 T at 340nm by ASTM E 387 A good indication as to whether the stray light level is within specification may be obtained as follows 1 Set the wavelength to 340nm 2 Set the MODE switch to T 3 With the square cell adapter in the sample compartment but no cell close the lid and press the 0A 100 T button to set the display to 100 0 4 Remove the cell adapter from the sample compartment and close the lid Make a note of the reading that should be at or near 00 0 5 Prepare solution containing 50gm L of sodium nitrite NaNO in distilled water and fill a square cuvette with this solution 6 Insert the cuvette into the adapter and place in the sample compartment Close the lid The display should read lt 0 5 T Note that if the reading obtained in step 4 is greater than 00 0 this value should be subtracted from the displayed reading to give the correct reading for the stray light value 15 Trouble Shooting PROBLEM Instrument Inoperative Possible Cause Power cord not connected to outlet Solution Plug instr
4. operator to re start at step 1 Mode Indicator MODE Button 0A 100 5 T T A Selects T Abs Button 3 UNICO 1100 SPECTROPHOTOMETER Sample Compartment Wavelength Control for 10mm Test Tubes Knob WAVELENGTH or 10mm Square and Wavelength Readout Cuvettes Model 1100 Spectrophotometer Mode Indicator Allows the operator to know the measurement mode currently in use Transmittance Absorbance MODE Button Switches between Transmittance and Absorbance operating modes at any time of measurement 0A 100 T Button Adjusts digital readout to 100 T or 0 000A when blank reference solution is in sample compartment Sample Compartment nsert 10mm test tube and close lid or fit 10mm cuvette adapter and fit adapter with holder to left of cuvette into compartment Wavelength Control Knob WAVELENGTH Selects desired wavelength in nanometers nm Wavelength Readout Window Displays desired wavelength 10 MODE OA 100 T 09 T Mode Indicator PEU Button T A F Print ENT Button Sample Compartment Wavelength Control for 10mm Test Tubes Knob WAVELENGTH or 10mm Square and Wavelength Readout Cuvettes Model 1100 5 Spectrophotometer Mode Indicator Allows the operator to know the measurement mode currently in use Transmittance Absorbance MODE Button Switches between Transmittance and Absorbance operating modes at any time of measurement 0A 100 T Button Adjusts digital readout to 100 T or 0
5. the packing list page 5 to ensure that you have received everything in good condition 2 Place the instrument in a suitable location away from direct sunlight In order to have the best performance from your instrument keep it as far as possible from any strong magnetic or electrical fields or any electrical device that may generate high frequency fields Set the unit up in an area that is free of dust corrosive gases and strong vibrations 3 Remove any obstructions or materials that could hinder the flow of air under and around the instrument 4 Select either 230V or 115V on the voltage selector switch illustrated below to match your local mains voltage supply 5 Turn on your Cole Parmer 1100 1100RS and allow it to warm up for 15 minutes before taking any readings Voltage Berahi Selector Switch Model 1100 Analog output Sich OV XT Uncontrolled Model 1100R5 R5232 Interface 8 pin null modem cable required Basic Operation Simple operating instructions are printed on the front panel of your Cole Parmer 1100 1100RS A 1 Prepare the spectrophotometer Turn on the spectrophotometer by pressing the Power Switch IO Allow 15 minutes for the instrument to warm up Select either the Transmittance or Absorbance operating mode by pressing the T A selector button MODE until the red light for T or A is on Select the desired wavelength by turning the wavelength control knob WAVELENGT
6. 000A when blank reference solution is in sample compartment 0 T Button When in transmittance T mode and sample compartment is empty a flap blocks the beam Pressing button adjusts readout to 00 096 T PRINT Button ENT First press after selecting Concentration mode enters reading on digital readout Other presses cause output of reading to printer If using Application Software press this button to communicate with software If operating in Factor F Mode then press to enter factor This causes the Mode to enter the factor number and change to Concentration mode Sample Compartment nsert 10mm test tube and close lid or fit 10mm cuvette adapter 11 and fit adapter with holder to left of cuvette into compartment Wavelength Control Knob WAVELENGTH Selects desired wavelength in nanometers nm Wavelength Readout Window Displays desired wavelength Maintenance A TO REPLACE HALOGEN LAMP 1 Turn off and unplug the instrument Turn the instrument upside down 2 Remove the grill plate on bottom of the instrument by removing fixing screw 3 Unplug the lamp from the white connector Insert the new lamp pushing it in as far as it will go Replacement lamp Part Number 1100 505 6V 20W G4 type CAUTION DO NOT HANDLE THE LAMP WITH BARE FINGERS USE TISSUE OR CLOTH WHEN HANDLING LAMP 4 Turn on the instrument Set the wavelength at 340nm insert a test tube or a empty cuvette with cuvette holder blank the instrument
7. AND SAFETY AT WORK ACT 1974 Read the following before installing and using the instrument and its accessories General The apparatus described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory For the correct and safe use of this apparatus it is essential that laboratory personnel follow generally accepted safe procedures in addition to the safety precautions called for in this manual The covers on this instrument may be removed for servicing However the inside of the power supply unit is a hazardous area and its cover should not be removed under any circumstances There are no serviceable components inside this power supply unit Some of the chemicals used in spectrophotometry are corrosive and or inflammable and samples may be radioactive toxic or potentially infective Care should be taken to follow the normal laboratory procedures for handling chemicals and samples Electrical Before switching on the apparatus make sure it is set to the voltage of the local power supply see Installation The mains plug shall be inserted in a socket provided with a protective earth contact The protective action must not be negated by the use of an extension cord without a protective conductor Warning Any interruption of the protective conductor inside or outside the apparatus or disconnection of the protective earth terminal is likely to make the apparatus dangerous Intentional inter
8. Cole Parmer 1100 SERIES SPECTROPHOTOMETER USER S MANUAL armer Instruments Company 625 East Bunker Court Vernon Hills IL 60061 800 323 4340 TABLE OF CONTENTS Page number Safety General Information 3 OM e H 4 Working Principle EE Me OE D 5 Unpacking Instructions ss as nn ne tte ne 5 Specifications ES eet 6 Msta O E 6 Components of 1100 1100RS backside Basic Operatio m SERM E cm 7 Sample Preparation and Analysis 7 Additional Features of 1100RS Concentration E RE pe eae SEA UE 8 Factor Mode MON NC 9 Model 1100 Nomenclature 10 Model 1100RS Nomenclature 11 Maintenance Lamp replacement 12 Wavelength Calibration Check 12 Absorbance Accuracy Checks 14 Stray Light CHEEK zc sn opo hid ibl oia dace deat beads 15 Troubleshooting cL 16 Safety This apparatus has been designed and tested in accordance with EN 61010 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use and has been supplied in a safe condition The safety statements in this manual comply with the requirements of the HEALTH
9. H Prepare Sample Make a blank reference solution by filling a clean cuvette or test tube half full with distilled or de ionized water or other specified solvent Wipe the cuvette with tissue to remove the fingerprints and droplets of liquid Fit the blank cuvette into the square cuvette adapter and place the adapter in the sample compartment aligning the guide mark if present with the guide mark at the front of the compartment Close the lid Set 0 000A 100 T with the 0A 10096T control button NOTE This step fulfills the instruction on the front of the spectrophotometer Set Full Scale Remove the blank cuvette or test tube Set it aside in the case that you may need to adjust the 0A 100 T control button later i e change the wavelength Analyze Sample Rinse a second cuvette with a small amount of the sample solution to be tested Fill the cuvette half full and wipe it Put the sample cuvette in the sample compartment Close the lid Read the Transmittance or Absorbance from the digital readout window Remove the sample cuvette or test tube If you are to test the same sample at other wavelengths repeat steps 3 to 10 for each wavelength For each new sample you analyze repeat steps 2 to 11 Additional Features of Model 1100RS Although operating basically the same as the Model 1100 the 1100RS spectrophotometer offers two more features Concentration Mode and Factor Mode as follows Concentration C
10. button Wait a few seconds while the display flashes BLA The reading should then be 0 0004 If not repeat step 11 12 Remove the cuvette holder and insert the Didymium filter into it Place it in the sample compartment and close the lid 13 Record the absorbance reading on the digital display 14 Advance the wavelength setting by 1nm and repeat steps 10 to 13 15 Repeat step 14 until the wavelength setting reaches 536nm Again look for the maximum absorbance reading It should be between 527 and 531nm Absorbance Accuracy Checks Specification 2 at 1A 1100 1 at 1A and 2A 1100RS The absorbance accuracy should be checked against a set of neutral density filters accurately calibrated to the NIST standards An alternative method using potassium dichromate is described below Due to the many factors that might affect the results i e temperature bandpass weighing and diluting errors this method is less accurate and should only be used as a guide Reference Johnson E A Potassium Dichromate as an absorbance standard PSG Bulletin 1967 No 17 page 505 1 Make up N 100 sulfuric acid as the solvent and use part of it to make a solution containing 120 0 5mg litre of potassium dichromate 2 Wash out a square cuvette with solvent and fill with solvent 3 Put the cuvette in the adapter into the sample compartment and close the lid 4 Set the wavelength to 350nm 5 Set the MODE button to A 6 Set the reading to
11. ents are ideal for measurements in the visible wavelength region of the electromagnetic spectrum Working Principle The spectrophotometer consists of five parts 1 Halogen lamp to supply the light 2 A Monochromator to isolate the wavelength of interest and eliminate the unwanted second order radiation 3 A sample compartment to accommodate the sample solution 4 A detector to receive the transmitted light and convert it to an electrical signal and 5 A digital display to indicate absorbance or transmittance The block diagram below illustrates the relationship between these parts Block diagram for the Spectrophotometer A QT 100 T Tel 2 Light Mono Sample Detector Display Source chromator Compartment In your spectrophotometer light from the lamp is focused on the entrance slit of the monochromator where the collimating mirror directs the beam onto the grating The grating disperses the light beam to produce the spectrum a portion of which is focused on the exit slit of the monochromator by a collimating mirror From here the beam is passed to a sample compartment through one of the filters which helps to eliminate unwanted second order radiation from the diffraction grating Upon leaving the sample compartment the beam is passed to the silicon photodiode detector and causes the detector to produce an electrical signal that is displayed on the digital display The Cole Parmer 1100 incorporates a special analog output
12. g Insufficient sample volume Fill cuvette with more sample Stray sample preparation vapors Prepare the sample away from the instrument Use proper ventilation Bubbles or particles in solution Check sample preparation and analytical procedure Defective electronic component or loose Call an authorized service engineer wiring Instrument drift and noise Lamp not adjusted Check lamp has been properly properly installed or has moved during transit Refer to lamp replacement instructions in this manual Lamp old or defective Replace with a new lamp Refer to lamp replacement instructions in this manual Defective or dirty detector or defective electronic component Call an authorized service engineer Incorrect readings obtained Insufficient sample volume Fill cuvette with more sample Wrong wavelength setting Check analytical procedure and wavelength setting Check wavelength accuracy according to procedure in this manual Stray sample preparation vapors Prepare sample away from instrument Use proper ventilation Bubbles or particles in solution Check sample preparation and analytical procedure Instrument out of electronic calibration Call an authorized service engineer 17
13. ruption is prohibited Whenever it is likely that the protection has been impaired the apparatus shall be made inoperative and be secured against any unintended operation The protection is likely to be impaired if for example the apparatus e Shows visible damage Fails to perform the intended measurements Has been subjected to prolonged storage under unfavorable conditions Has been subjected to severe transport stresses Performance To ensure that the instrument is working within its specification especially when making measurements of an important nature carry out performance checks with particular reference to wavelength and absorbance accuracy Performance checks are detailed in this manual Radio Interference For compliance with the EMC standards referred to in the EC Declaration of Conformity itis necessary that only shielded cables supplied by Cole Parmer be used when connecting the instrument to computers and accessories Introduction The Cole Parmer Model s 1100 and 1100RS are single beam general purpose instruments designed to meet the needs of the Conventional Laboratory The Cole Parmer 1100 is ideal for various applications such as Clinical Chemistry Biochemistry Petrochemistry Environmental Protection Food and Beverage Labs Water and Waste Water Labs and other fields of quality control Featuring a digital display of photometric result easy operation and wavelength range of 335nm to 1000nm these instrum
14. terminal that can be used to interface the spectrophotometer with a chart recorder or with any computer interface that will accept a signal which varies from 0 1 0 Volt DC Unpacking Instructions Carefully unpack the contents and check the materials against the following packing list to ensure that you have received everything in good condition Packing List Description Quantity SDeGIFODhOLIOIiBlol 5 nn SAS 1 POWELL tolo i drip adepta Maat qe dope pea oe Pv EDU sete tae Mut Era 1 GUVelles o eee ra t et Re AN E ea ia Box of 12 Square Cuvette AOSDIGOF SEP oet Rede e addy annales 1 DUS GOVE A EEE idu iced IP e ebs 1 LIES ARTT RR RR ERE 1 Specifications Model 1100 Model 1100RS Wavelength Range 335 1000nm 335 1000nm Spectral Bandpass 20nm 10nm Wavelength Accuracy Better than 2nm Better than 1nm Wavelength Repeatability Better than 1nm Better than 1nm Stray Radiant Energy 0 5 340 and 400nm 0 5 340 and 400nm Photometric Range 0 to 125 T 0 to 125 T 0 to 2 0 Abs 0 to 2 0 Abs 0 to 1999C Photometric Accuracy 2 0 T 1 0 T Power Requirements 115 230V 10 60 50 Hz 115 230V 10 60 50 Hz Dimensions 408W x 308D x 185H mm 408W x 308D x 185H mm Weight 6kg 6kg Installation 1 After carefully unpacking the contents check the materials with
15. the cuvette holder and insert the Holmium filter into it Place it in the sample compartment and close the lid Record the Absorbance reading on the digital display Advance the wavelength setting by 1nm and repeat steps 4 to 7 Repeat step 8 until the wavelength setting reaches 370nm Look for the maximum absorbance reading obtained and this should be found between 359 and 363nm The wavelength accuracy of the 1100 1100RS is 2nm Didymium Filter Method 1 2 Set the Wavelength to 800 nm Make sure the cuvette holder is empty and place it in the sample compartment Close the sample compartment lid Set zero Abs by pressing the 0A 100 T Wait a few seconds while the display flashes BLA The reading should then be 0 0004 If not repeat step Remove the cuvette holder and insert the Didymium filter into it Place it in the sample compartment and close the lid Record the Absorbance reading on the digital display Advance the wavelength setting by 1nm and repeat steps 2 to 5 Repeat step 6 until the wavelength setting reaches 815nm Look for the maximum absorbance reading obtained and this should be found between 805 and 809nm The wavelength accuracy of the 1100 1100RS is 2nm If a middle wavelength check is desired set the wavelength to 522nm optional 13 10 Make sure the cuvette holder is empty and place it in the sample compartment Close the sample lid 11 Set zero Abs by pressing the 0A 100 T
16. ument in Dead Power outlet Change to a different outlet Internal fuse blown or defective electronic component Call an authorized service engineer Instrument cannot set 100 T 0 000A Light beam blocked Check sample holder Test tube or square cuvette adapter must be in sample compartment to open sample holder shutter Lamp is old or defective Replace lamp Hefer to lamp replacement instructions in this manual Instrument cannot set 100 T 0 000A T cannot be set to 00 0 Defective electronic component Sample holder Call an authorized service engineer Hemove cuvette holder or test tube Sample holder shutter May be stuck open Close shutter Defective electronic component Call an authorized service engineer Incorrect Transmittance to Absorbance correlation Bubbles or particles in solution Check sample preparation and analytical procedure Defective electronic component Call an authorized service engineer Digital Display does not change regardless of sample concentration Concentration reading frozen MODE switch has been changed from C to F T or A and back to C Restart measurement procedure 16 Problem Possible Cause Solution Digital Display does not change regardless of sample concentration continued Wrong wavelength setting Check sample procedure and wavelength settin
17. uring all the samples change the Mode to Factor F and read the multiplier from the display Keep a record of this value for future use Operational Note if the mode switch is changed to read Factor or Absorbance the Concentration C reading is frozen and cannot be changed This requires the operator to re start at step 1 Factor F Mode This is a special mode for measuring concentration values of unknown samples using a previously determined factor to convert Absorbance readings to concentration 1 After setting the wavelength and setting zero Abs on the blank solution using the MODE button select factor F mode 2 Insert a cuvette containing a sample 3 Using the Aand v arrows set the digital read out window to the desired value of the multiplier 4 Press the ENT button The spectrophotometer switches to the Concentration C MODE Operational Note If the Concentration of the sample is too high to be displayed the instrument will not switch to Concentration Mode when the ENT button is pressed Dilute the sample and multiply the concentration reading by the dilution factor to obtain the original sample concentration 5 Read the concentration value of the sample direct from the display 6 Insert a cuvette containing the next sample and read the result Repeat until all samples have been measured Operational Note If the MODE switch is changed to A or T then the concentration reading is frozen This requires the
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