Hotlink to MACSQuant Automated Labeling and Multisample
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1. FLT 3 jLD45 VioBlue FL2 FITC FLE ICO15 AFL FLS CD14 PE FLY APC Cy Figure 1 MC CD14 Cocktail is selected for autolabeling The corresponding annotations have been uploaded Are you in need of additional assistance Visit www miltenyibiotec com local to find your nearest Miltenyi Biotec contact Miltenyi Biotec provides products and services worldwide Visit www miltenyibiotec com local to find your nearest Miltenyi Biotec contact MACS MACSQuant and MACSQuantify are registered trademarks or trademarks of Miltenyi Biotec GmbH Unless otherwise specifically indicated Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use Copyright 2010 Miltenyi Biotec GmbH All rights reserved 140 003 1482. Miltenyi Biotec Excite and inspire Automated labeling and multisample processing MACSQuant Analyzer quick guide Description Multisample processing on the MACSQuant Analyzer is accomplished with the MACS MiniSampler combined with the MACS Reagent Rack 4 and MACS Cooling Tube Racks The most common racks used for multisampling are Chill 5 and Chill 96 allowing for processing of up to 96 samples in a single batch Several different types of cell samples or analysis panels can be configured on the same rack Samples can also be automatically labeled with fluorochrome conjugated antibodies cocktails or fluorescent dyes prior to measurement This quick guide walks you through the steps to select positions in a Chill Rack and upload reagents in the reagent manager Overview of the Cooling Tube Racks Chill 5 Chill 96 rack 96 rack Slots 24x5 mL 96 well microtiter plate PHONON MACSQuantify Rack drop down list Corresponding TE a MACSQuantify A TANG i pena haimaa rack graphic mj aj wel es J Table 1 Common Cooling Tube Racks used for multisample processing on the MACSQuant Analyzer Configuring a rack To select a sample rack 1 Click on the Experiment tab 2 Select the required rack type from the Rack dropdown list 3 The corresponding rack is now displayed in bottom left corner A layer version of rack will also be displayed in middle of screen If not one can be opened by clicking
3. the l icon To select rack positions 1 Use the left mouse button to activate the desired well alternatively use the touchscreen 2 Wells can be selected and deselected by reclicking the well 3 Only wells in selected state can be programmed using the Experiment tab 4 Multiple wells column row rack can be selected by using the right mouse button while clicking on a Upper left circle for entire rack b Column number for entire column c Row letter for entire row 5 Refer to table 2 for overview of rack configuration and to section 6 7 2 in the user manual for more information 6 Use the Experiment tab to change parameters of the experiment flow rate uptake volumes etc and Custom instrument settings analysis templates or Express MC cocktail analysis templates settings 7 Refer to the MACSQuant Analyzer quick guide Setting up an experiment for data acquisition in custom mode or the user manual for more details User action with Effect left mouse button Details or fingertip action on touch screen None Single click on circle single finger touch Double click on circle double finger touch Default open circle indicates no operation Clear Closed green circle with orange rim Sample selected for measurement Orange circle indicates that the sample is activated and any alterations made to the measurement strategy e g labeling
4. annotations are automatically adjusted according to the MACS Reagents that have been selected Miltenyi Biotec Configuring autolabeling parameters for rack positions 1 With the designated rack open refer above to To select a sample rack select wells for autolabeling With the well s in selected mode click the reagent s required for autolabeling that sample refer to figure 1 from the Autolabel tab Repeat for other samples if samples require different reagents for autolabeling If using MC Cocktails for autolabeling a predefined analysis template can also be assigned to the wells using Express mode under the Settings tab Before starting the run check that 1 The experiment definitions are correctly assigned to each well and samples are placed accordingly Sufficient quantities of reagents and buffers are provided and that the waste bottle is empty The reagents have been assigned in the reagent manager and placed without cap on reagent rack The instrument is correctly calibrated and compensated for the samples Note Use the Experiment Table under the View menu to quickly confirm the experimental set up Annotations Autolabel Settings R MC CD14 T lt add lt add gt P add gt lt add gt lt add lt add gt lt add gt lt add gt M lt add gt Annotations Autolabel Settings FL5 PE Cy
5. will only apply to sample positions with this designation Closed green circle Sample selected for measurement None Closed blue circle Measurement in progress None a amp Closed gray circle bd Measurement finished None D Closed yellow circle Processing of sample has commenced e g sample has been labeled and incubation is underway Table 2 Summary of rack configurations Defining automated labeling configurations Setting up the reagent manager 1 Open the reagent manager by selecting Reagents under the Edit menu or by clicking add in the Auto label tab Select reagent rack position R1 R2 etc Select the reagent from the category and reagent dropdown lists Alternatively scan the reagent using the barcode reader Note Propidum iodide PI is listed under Universal Samples can be automatically diluted prior to measurement by selecting a buffer dilution from S1 or S2 Define time titer and order for incubation Note MACS MC Cocktails and fluorochrome conjugated antibodies have predefined time and titer Order is used if more than one reagent might be added to the same sample but requires different incubation times e g antibody and PI Once all reagents are uploaded a total of four reagents buffer A or B click Apply In the Autolabel tab see the uploaded reagents available to apply to the rack positions figure 1 Note The
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