Getting Started with UNICORN 5.0
Contents
1. e change method instructions to adapt to non standard system configurations e create new methods for applications not covered by the supplied templates or wizards The system strategy determines the available instructions A method that is developed for one system may not be valid for another The table below describes how to open the Text Instructions Editor Step Action 1 Select the Method Editor module 2 Select File New Result The New Method dialog opens 3 e Select a system if more than one is available e Select Method Editor and click OK Result The Method Editor module opens in text edit mode 4 Proceed with step 5 and 6 if the screen is blank 5 Click the Customise Panes icon ae e p27 4 How to create a method 4 2 How to use text instructions Step Action 6 Select Text and Instruction Box and click OK Text Flow scheme Instruction box B KK K K K Cancel Help How to enter Text The text instructions are selected in the Instruction box in the lower part of the Instructions Method Editor Applicable parameters can be edited for each selection Use the buttons to Insert Change Replace or Delete the selected instructions All text entries are shown in the Text pane The illustration below shows the Instruction box me EN Leo 03 0014 92 ep28 How to perform method runs 5 5 How to perform method runs Introducti2. The Customise Report window opens 4 Proceed to How to add items to the empty report How to add items The table below describes how to add items to your empty report format to the report Step Action 1 Select an information item e Click one of the item icons in the toolbar or e Select an item from the Insert menu GSAS Layout Help T Free text Text Method E Chromatogram Documentation a Evaluation log Quantitate and Mol Size ES Frac 950 ep l 10 How to create and print reports 10 3 How to create and print a customized report format The Setup Chro matogram dialog box How to set up the page format 03 0014 92 ep62 Step Action Press and hold down the left mouse button and drag out a box to the size of the item you want to insert Note The mouse pointer shows a symbol for the type of item you have selected Release the mouse button Result A Setup dialog box opens The dialog box is specific to the type of item that you want to insert CE The illustration below shows the Setup Chromatogram dialog box in the Customise Report window EG 7 DINERS sob dalada Double click anywhere in the Customise Report window Result The Page Setup dialog box opens Click the Page Setup tab and enter values for all Margins 3 Click the First Header tab and select the items that you want to in clude in the f
3. This section is an overview of the contents and the relationship between the manuals The manuals The three manuals are e Getting Started with UNICORN See section 1 2 About this manual on page 8 e UNICORN User Reference Manual e UNICORN Administration and Technical Manual User info about The questions and answers in the table below describe the features of the Getting Getting Started Started manual Question Answer Who should read Getting Started Users that are new to the UNICORN system and with limited experience from other chromatography systems What do I need before I start A basic knowledge of PC and Windows functions and an understand ing of the concepts and terminology of liquid chromatography What are the contents of Getting Basic descriptions of UNICORN and Started its use based on a model system How should I use Getting Started Read in front of your computer and test the instructions at the same time When do I need to refer to the User When you need Reference Manual e more in depth information e alternative ways to perform a task When do I need to refer to the Admin When you need f R j istration and Technical Manual e general information about the net work functions of UNICORN e to set up and configure a UNICORN workstation in a net work e totrouble shoot a workstation with network problems 03 0014 92 ep 10 Introducing UNICORN 1 User info about The
4. about The questions and answers in the table below describes the features of the Administration and Technical Manual Who should read the Administration What are the contents of the Adminis How should I use the Administration When do I need to refer to Getting When do I need to refer to the User Answer System administrators e General knowledge of UNICORN e Knowledge of PC Windows and general network administration functions e An understanding of the concepts and terminology of liquid chroma tography Detailed instructions of e how to install and maintain UNICORN in a network environ ment e how to create and administrate user profiles Note Most instructions are based on a model system e If you are an experienced adminis trator of previous UNICORN ver sions you can read selected sections for reference e If this is your first experience of UNICORN administration we re commend that you study the manual in detail When you need brief step by step in structions for a selected task When you need more information about the basic functions of UNICORN The Administra tion and Technical Manual Question and Technical Manual What do I need before I start tration and Technical Manual and Technical Manual Started Reference Manual 03 0014 92 ep 12 1 4 Introduction Quick Start in structions Introducing UNICORN 1 Quick Start Guide This gui
5. cut and paste e right click short cut menus Note Drag and drop is not available File and folder handling in UNICORN also differs from the general Windows file manager standard UNICORN can be used with a number of systems including e AKTA design systems e BioProcess systems Note All examples in this guide are based on an AKT Aexplorer 100 system that operates with the E100F400 strategy If you use another system you may find that the descriptions and instructions do not match your system on every point In that case you also need to refer to the user documentation for your specific chromatography system Introducing UNICORN 1 System networks UNICORN can be installed on a stand alone computer to control only a single locally attached system However a stand alone computer can control up to a maximum of four separate systems In a network installation each computer workstation can operate many systems regardless if they are locally connected or not Each system can only be operated by one workstation at a time but several may view the output data E E Software modules The UNICORN control software consists of four integrated modules Module UNICORN Manager Method Editor System Control Evaluation Function File handling and administration e g definition of systems and user profiles etc To create and edit methods for pre programmed control of chromato graphy systems To control a
6. questions and answers in the table below describes the features of the User the User Reference Reference Manual Manual Question Who should read the User Reference Manual What do I need before I start What are the contents of the User Reference Manual How should I use the User Reference Manual When do I need to refer to Getting Started When do I need to refer to the Admin istration and Technical Manual Answer e Users that are experienced with previous UNICORN system ver sions e Users with vast experience from other chromatography systems e Knowledge of PC and Windows functions e An understanding of the concepts and terminology of liquid chroma tography e Preferably some previous experi ence with UNICORN e Detailed descriptions of UNICORN e Instructions on how to use the sys tem with suggested alternatives Note Most instructions are based on a model system Depending on your previous experi ence you can either read e whole chapters from the beginning to the end e only selected sections for reference When you need brief step by step in structions for a selected task When you need e general information about the net work functions of UNICORN e to set up and configure a UNICORN workstation in a net work e totrouble shoot a workstation with network problems epll 1 Introducing UNICORN 1 3 About the UNICORN user documentation User info
7. the Evaluation module The Raw Chroma The result file is opened in a Chromatogram window The default view shows all togram Data the curves as in the illustration below juanta 0t OS n iE AR D OAO AA Dr Gaa 1 Cana i603 Qwartrtal ALWOOL I Cons JIII UI rtt 1 Progne hi I 3POUantt OO Flow m a JIO Rita toDO 1 Temp 1030 anlitateDO ES njuct juarrbtateOO 1 1 AI OnO SASEM i633 QcartitateOO8 1_Logbeck How to change the chromatogram layout Step Action 1 Right click in the Chromatogram window and select Properties Result The Chromatogram Layout dialog box opens 2 Click the Curve tab 3 Click the check boxes to de select the curves that you do not want to display e p43 7 How to view results 7 1 How to view and edit your result layout Raw data curves are saved How to change the Y axis scale How to change the Y axis scale display How to view curve details 03 0014 92 ep44 Step Action 4 Click OK Your selections determine the curves that are displayed in the Chromatogram window and shown on printouts Note The original raw data curves can never be modified renamed or deleted from the result file Normally the curves are auto scaled i e the highest and lowest values for each curve set the scale The table below describes how to change auto scaled Y axis scales to fixed values in the Chromatogram Layout dialog bo
8. 950 setup dialog box you can define the order of fractionation and set up the last tube The system will be paused when the last tube is reached and the fractionation will stop The illustration below shows the Frac 950 setup dialog box Frac 950 ix ae 0000 sealer 000000000000 en 200900000000 ED ES 000000000000 E E 800008000680 sm Wy ly 000000000000 000000000000 acan 000002000000 E slalalelateletetetetete E 000000000000 0000 C The final step before starting is to name your result file and define where it should be stored The default file name is defined in the Result Name page of the Run Setup By default the result file name will either be the same name as the method the date of the run or a pre defined name The name is followed by a three digit sequence number starting with 001 You can change this name and select a new directory by clicking Browse You can also select to have the software add a unique identifier to the file name ep3l 5 How to perform method runs 5 1 How to run your method How to start the Click the START button in the Result Name dialog box This will initiate the method method run run and you can follow its progress in the System Control module 03 0014 92 ep32 9 2 Introduction How to select dis plays The Run Data pane How to perform method runs 5 The System Control module All data on your separation run are displayed in the System Con
9. F AveragngTimeUV 2 55 sec C Tine nd Block Volume lock Eluent Inlet Pumg lniet AT End Block Block Eluent E Inket Base SemeAsMain Epi 81 Block Stan w h Purcwath Pure Base SameAsMan Pane Oe OFF OFF OFF Fics E zi g Adjust retenon zeo merta 2SS9999S99999S99999S5999595 B8888888888888sssssssssss SSS SIS 22 a r EEEN 03 0014 92 ep 46 7 3 Introduction Open the dialog box The Open curves to compare dialog box How to import the curves How to view results 7 How to compare curves You can import or copy curves from different method runs into one chromatogram for comparison This section is an example of how you can use the function Open to compare to import curves First open the dialog box Open Curves to Compare You can either e Select File Open to compare Curves or e Click the Open curves to compare icon The illustration below shows the dialog box Open Curves to Compare Chromatogram selection Folder fe Defauts Browse Result i 3 Browse All Chromatogram Po M Browse All EE Curve name IR bs Browse All r Found curves 7 Clear v _Seectat ATJMFO06 1_UV1_215nm JBaseline example 1 ATJMFOOE 1_Cond Select All Baseline example 1 ATJMFOO6 1_Cond I Baseline example 1 ATJMFOOE 1_Cone Baseline example 1 ATJMFOOE 1_pH gt Curve options C Overlay Stack Miror T S
10. a Scouting SCHEME ciewtasvarsoriaiateactetavuies cradalo ana Lisa ea a nne 40 es HOW LO VIEW FOS MNES assassinar ndo ad dE San avert tut a Sa Ro owner Cece Saa aa ni Ea 42 7 1 How to view and edit your result JAQUE reiiesccsariscetiesbbidelasiatedsevelenetebucneadenteistedens 43 7 2 How to view the run GOCUMENLATION asics seccuass say veaeeadiiverbs ceahds engssaantnbeteacsantsbaraeten 46 Td OW LO compare Curves oaea TS geet Sa owen 47 8 How to print your ChromatOgrams ccccceesesssseeeeeeeeeeseeeeeeeeeeesssseeeeeeeeseeseeeeeeeeensenaaes 49 8 1 How to print active Enromalograms s 2 sena deed orar dada AS DS 50 9 How to evaluate and save the reSults cccceceeeeeeeesesseeseeseeeeeeeeneeeeeeeeeeeeeeeeeeeeeneeeeseeeneeees 52 9 1 How to perform a basic peak Integration ss sussanga as serasa ered arate aids ag eres 53 9 2 HOW t save the TESUITS ispiser ra A ea uae ae area 56 10 How to create and print reports c sccsceeessessseeeeeeeeesseeeeeeeeeesseseeeeeeeeeeeeesseeeeeeennenaaes 57 10 1 How to print an existing report formal aneosisars lince resina na sh nin aan sina aa dio anna 58 10 2 How to edit an existing report forMat cccccceecseccseseeesseeeeseeeeeneeeeeereeeneseneenaees 59 10 3 How to create and print a customized report format ccccececseceeeeeeeeeeeneenaees 61 Table of Contents 03 0014 92 epii 1 Introducing UNICORN Introducing UNICORN 1 About this chapte
11. arked Excluded and will not be executed It will still remain in the scouting scheme to insert a new run select the column to the left of the position where you want to insert the new column and click the Insert button Result A new column is added immediately to the right of the selected column to define new scouting Click the Define button variables Result The Scouting Variables dialog box opens When the method is started the scouting runs are performed automatically Each method run in the Scouting Scheme generates a separate result file which is stored in a special scouting directory ep4l 7 How to view results 7 How to view results Introduction This chapter describes how to view the results from your method run in the Evaluation module In this chapter This chapter contains the sections below Topic See How to view and edit a result layout 74 How to view the run documentation 72 How to compare Curves 7 3 03 0014 92 ep 42 How to view results 7 7 1 How to view and edit your result layout Introduction This section describes the basics of how to view and edit the layout of your results in the Evaluation module How to open the The table below describes how to view the results result file Step Action 1 Complete the method run 2 Locate the result file in the Results pane in the UNICORN Manager module 3 Double click the file Result The file opens in
12. de is intended for users who are fully familiar with the safety precautions and operating instructions that are described in all manuals i e experienced users of previous versions of UNICORN The instructions assume that all installations were made according to the instructions that the model system is used and is connected The table below describes the easiest way to create a method run the system and generate a printed chromatogram The procedure is based on an Instant run Step 1 Action Click the Instant run icon in the UNICORN Manager Ee Result The Instant run dialog box opens e Select Wizard e Select a system if necessary e Click the Run button Result The Method Wizard opens in the System Control module Go through all selections on the Method Wizard pages Click the Next button to proceed from page to page Click the Run button on the last page Result The start protocol opens Verify the method on the Variables page and change values as re quired Click the Next button to proceed through several pages Select Print Chromatogram in the Evaluation procedures page Result A printout will automatically be generated after the run Click the Start button on the last page Result The run starts ep13 2 UNICORN concepts 2 UNICORN concepts Introduction This chapter contains definitions and descriptions of some of the specific concepts that are presented in this man
13. e 57 if you want to add text information to your prints or create a complete report In this chapter This chapter contains the section below See Topic How to print active chromatograms 81 e p49 8 How to print your chromatograms 8 1 How to print active chromatograms 8 1 How to print active chromatograms Introduction This section describes how to print the chromatograms that are open in the Evaluation module Before you print Open all chromatograms that you want to print in the Evaluation module before you proceed How to print The table below describes how to print active chromatograms Step Action e Select the File Print menu command or e Click the Print icon Result The Print Chromatograms dialog box opens Select print formats and layout options Click Preview Result The Customise Report window opens e Verify that the layout is correct e Click Edit Mode to make changes e g change the order of the chromatograms Click Preview to return to preview mode e Click Exitto return to the Print Chromatograms dialog box 5 Click OK 03 0014 92 ep 50 How to print your chromatograms 8 The Print Chroma This is an illustration of the Print Chromatograms dialog box tograms dialog i box Note The selected print format is outlined in red Print Chromatograms ep5l 9 How to evaluate and save the results Introduction In th
14. e selected items will be displayed in the peak table 5 Click OK This is an illustration of the Peak Table dialog box Chromatogram Layout 1 x Curve Style and Colour Edit Texts Layout Library Header Curve Names Y Axis Axis Curve Peak Table Select peak table to display Select peak table columns Peak bl d14 1 1 UM are eaktable id 48Quantitate001 1_UV1_ 280nm 0 AR Peaktable B Siera Peaktable C Hwi Peaktable D M rea Peaktable E Height rp Peak endpoint retentions eaktable G e k Peaktable H width at half height Percent of total area 4 gt Percent of total peak area r Filter Peaks m Fill settings T Height less than fo E I Width less than po Colour Area less than o Pattern aaa T Width more than fo IRA ANN IV Peak must be one of 20 largest Sa I Apply to all chromatograms Cancel Help e p55 9 How to evaluate and save the results 9 2 Howto save the results 9 2 Introduction How to delete un wanted curves How to save the results 03 0014 92 e p56 How to save the results After you have finished the evaluation process you can save all the changes you have made to the chromatograms including newly created curves and chromatograms that you have imported and created All the curves that you created during your manipulations will be saved in the chromatogram Some of these cur
15. ense Agreement Copyright Amersham Biosciences AB 2004 All rights reserved Table of Contents Table of Contents 1 Introducing UNICORN ces sassasssesaiesseniiaissaoiean ar ar basis sra Gral senda andas deparar iia 3 Lo About UNICO N asi D abas RD SEER Eno gi extent DS a ED TT Dai 4 1 2 About thisman a k sesta sa te E r aa i 8 1 3 About the UNICORN user documentation cc iii eeerererererree era rerarea 10 LA Quek Start GUS a ie E a EEOAE ANAE E 13 2 UNICORN CONCONIS vicnsecnccwcceaserescatecwesccedccsactudedsaseaaceidantudubsncecseneoen Seectcecee devsdisvhvdeecaudieesaus 14 2 CONCEDE CET INN LIONS esa a rag E E 15 3 How to start your System sssssssssssnnunnnnnnnnnrnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnmnnn nnaman 18 3 1 How to start UNICORN and log Ollie pa o ces arena a E Ravascum samen 19 4 How to create a methodi idia a acu eA eas 21 4 1 How to use the Method Wizard ads da O L indent 22 4 2 How to use text IMSTFUCTIONS usina ai rasas aa a 27 5 How to perform method runS sssssssssssnnnnnnnnnnennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn eae aii asa 29 5 1 How to run your GA L 106 of PAN RAN RR RP DEAD RAR DARDO EGOR RA AN EEEn 30 5 2 The System Control MOGU Cis cite rn nan Sa Gaga ranr n rnaner nn 33 5 3 How to change the way your run is GiSplayed ccccececeseeeeeeeeee eens eeeeerseeeeeeeeenes 35 5 4 Manual Contolera ne orena A O a 37 Bs SCOUTING ROMERO a E EA A SA 39 6 1 How to set up
16. ers to the questions from the Run Setup Questions page Click Print Result The Print dialog box opens Click OK The Generate Re This is an illustration of the Generate Report dialog box port dialog box Generate Report xi Format Contents From sesuk Chomatofocusing Customised Report Format Global BP Chromatogr Report Global BP Ful Report al 5 Global Cheomatogram Peaks Global Cheomatogram Quark Global Cheomatogram Report Global Ful Report Global Installation Test 03 0014 92 ep 58 How to create and print reports 10 10 2 How to edit an existing report format Introduction This section describes how to open an existing report format to edit the items that are included in the report How to open and The table below describes how to open and edit a report format edit a report format f Step Action 1 e Choose File Report or e Click the Report icon a Result The Generate Report dialog box opens Generate Report x N Format Contents From resul Cheomatofocusing Global BP Chromatogr Report Global BP Ful Report Global Chromatofocusi Customised Report Format Global Cheomatogram Peaks Global Cheomatogram Quark Global Cheomatogram Report Global Ful Report Global Installation Test Print Preview Close 2 Select a report format 3 e Click Edit Result The Customize Report window opens
17. g tab in the Run Setup Result A Scouting Variables dialog box opens e Click the checkbox for the variable Flow Rate and any other variable you wish to scout e g BufferPrep pH e Click OK Result The selected scouting variables will appear in a column Click Add to create a second column for the next run This column will have the same values as the previous column Change variable values in the same manner as on the Variables page See 4 1 How to use the Method Wizard on page 24 Repeat steps 3 and 4 until you have defined all the runs you require Select the File Save menu command The Scouting tab How to edit a Scouting Scheme The scouting res ults Scouting 6 The illustration below shows the Scouting tab in the Run Setup ReferenceCuves Evaluation Procedwes Method Infomation Stat Protocol Result Name Froc850 Variables Scouting Notes Questions Grader BultePiep Columns a fo Po Er a C A E a BulterPrep BultePrep cH 72000 esoo feso S Saar GEES Es 7 Display tooltip for extended scouting scheme cells Define Clear All Delete Insert Add Seis Eaves Help The table below describes how to edit a Scouting Scheme If you want then to select a run click the column header to delete a run select the column and click the Delete button Result The column is removed to exclude a run right click the column header Result The column is m
18. in edit mode 4 e Double click an item that you want to edit in the report Result A Setup dialog box specific for the item opens 5 e Edit the item e Repeat step 4 for all other items you want to edit ep59 10 How to create and print reports 10 2 How to edit an existing report format How to apply the Once you have finished editing the report items you can apply the format report format If you want to then to print the report e click the Print icon amp to preview the changed click the Preview button report layout to save the format e choose File Save As and enter a name for the report format 03 0014 92 ep 60 10 3 Introduction How to create a new report format How to create and print reports 10 How to create and print a customized report format You can select a number of different objects including chromatograms methods images free text etc and create a customized report format The objects can be aligned re sized and positioned to fit your specific layout This section describes only some of the formatting options The table below describes how to create a new customized report format Step Action e Choose File Report or e Click the Report icon a Result The Generate Report dialog box opens 2 Click the New button Result The Create New Report Format dialog box opens 3 Choose Customised Format and click OK Result
19. irst page header The Page Setup dialog box How to create and print reports 10 Step Action 4 Click the Footer tab and select the items that you want to include in the footer 5 Click the Header tab and select the items that you want to include in the header Note This tab is not available if you selected Same header on all pages in the Page Setup tab The illustration below shows the First Header tab in the Page Setup dialog box Page Setup x Page Setup First Header Header Footer The selected items will be included in the report r Select Items m Logo Current user Font MV Logo gromen Run user C Left Current date amp time Select All Browse C Middle MRun date amp time Bight Report title Result file name Clear All Layout MMethod file name d Page number nenger AL smut nama IV Line under Free text Font Report title UNICORN Report Font Cancel ep 63 03 0014 92 ep 64
20. is chapter 03 0014 92 e p52 How to evaluate and save the results This chapter describes how to perform basic evaluation procedures and how to save the results of the evaluations This chapter contains the sections below Topic See How to perform a basic peak integration How to save the results 9 2 9 1 Introduction Baseline calcula tions How to perform the peak integra tion How to evaluate and save the results 9 How to perform a basic peak integration Peak integration is used to identify and measure curve characteristics including peak areas retention times and peak widths A correct baseline must be calculated before the peak areas can be calculated There are several alternative ways to perform this calculation e use automatic calculation e subtract a blank run curve from the source curve e use a Zero baseline i e no baseline subtraction at all e re use an existing baseline e edit a baseline manually from any curve in the chromatogram The default options are used for the example in this section i e automatic calculation Calculate baseline with a Morphological algorithm The table below describes how to perform a basic peak integration Step 1 Action e Select the Integrate Peak Integrate menu command or e Click the Peak Integrate icon fil it Result The Integrate dialog box opens Select a source curve Select a peak tab
21. le destination from the Target peak table list Select Calculate baseline in the Baseline list Click OK ep 53 9 How to evaluate and save the results 9 1 Howto perform a basic peak integration The Integrate dia This is an illustration of the Integrate dialog box log box Peak integration results 03 0014 92 ep 54 02 125200101 1 UY2 250nm 03 125200101 1_U Y 3_Onm 04 125200101 1 Cond 05 125200101 1_Cond 06 125200101 1 Cone DZ 125200101 1 pH 08 125200101 1 Pressure 0S 120200101 Flow O 125200101 1_Temp is 125200101 1_SamplePres 14 125200101 1_SampleFlow Calculate baseline 73 5876 25 7677 79 2309 173 1411 Note The peak table is displayed underneath the active chromatogram The start point and end point of each peak are marked by vertical marks drop lines How to display peak characterist ICS The Peak Table dialog box How to evaluate and save the results 9 The peaks in the integrated chromatogram are automatically labelled with their retention times Several other peak characteristics are also calculated automatically The table below describes how to display other characteristics Step Action 1 Right click in the active chromatogram 2 Select Properties Result The Chromatogram Layout dialog box opens 3 Click the Peak Table tab 4 Select the options that you want to display from the Select peak table columns list Result Th
22. n Setup consists of a number of tab pages Click the tabs at the top of the dialog box to select a tab page Evaluation Procedures Method Information StetPiotocd Questions Resuk Name Frac 950 Variables Scouting Notes Gradient BufleiPrep Columns Reference Curves Block Variable Value Range an Column ml 0 100 0 100 899599 000 Foi Row Rate Gand fimo fomos Cem Presse tet Cohen Preset Pa feo Sooo Stenio fvimenghiiom fe fisco EE Eres Profa oo Probha fi TT TD Euse Fac see UE DIDO 9888 000 Doo TI ferskfmcsmin fdposicnt Taiget Conca ee A aT DO I Show details I Show unused variables I Display tooltip for extended variable cells E E E E E J E e E E E E E E heu Setup Vari The method is represented by a number of blocks on the Variables tab The blocks ables are typical steps in a chromatographic run Each block contains a number of Variables with suitable default values that can be changed to suit your application Some values are normally hidden Click the checkbox Show Details to display hidden variables Default values When you select a column default values will be set for e The correct column volume e The recommended flow rate e The correct pressure limit Note If you exceed the recommended values for the selected column you will receive a warning when you save your method 03 0014 92 ep 24 How to create a method 4 The G
23. n every point In that case you also need to refer to the user documentation for your specific chromatography system Refer to other The examples and instructions that are presented in this manual are deliberately manuals limited to a minimum to reduce the number of pages that you will need to read In almost all cases you will find additional information about each topic in the User Reference Manual Document struc The manual is divided into chapters Each chapter starts with a brief overview that ture presents the contents and the headings for the sections that the chapter contains The section begins with an introduction that summarizes the content A section is divided into blocks of information with separating lines The blocks are identified by labels in the margins This makes it easier for you to quickly scan a page to find the exact topic you are looking for The manual con Getting Started contains ten chapters that can be sorted logically into 4 major tenis topics Topic Chapter Background information 1 Introducing UNICORN 2 UNICORN concepts 03 0014 92 ep8 Typographical representations Pre requisites Introducing UNICORN 1 Topic Chapter How to prepare the system How to start your system How to create a method How to run the system How to perform method runs How to view results 3 4 5 6 Scouting How to use the results 7 8 How to print chromatograms 9 How
24. n many results Note Refer to the User Reference Manual for information about Batch runs The topic is not covered in this manual BufferPrep is a function to prepare a buffer of different pH and salt concentrations online from four stock solutions This eliminates the need to manually prepare new buffers every time the pH needs to be changed Note BufferPrep is only available for some AKTAdesign systems Refer to the User Reference Manual for information about BufferPrep The topic is not covered in this manual A chromatogram is a collection of data represented by a number of curves that have been created during a separation run including UV conductivity pH fraction marks etc The original raw data curves cannot be deleted or modified They can be used as a basis for evaluation procedures and subsequent creation of new curves A chromatogram can also contain curves that have been created and saved during an evaluation session The monitor signals from the chromatography run are displayed graphically as curves epl5 2 UNICORN concepts 2 1 Concept definitions Method MethodQueue Method Wizard Result files Scouting 03 0014 92 ep 16 The program instructions for a chromatography run are defined in a Method A Method is normally divided into blocks that represent steps in the separation process Each block consists of a series of instructions that request specific operations in the system MethodQ
25. n you work in the UNICORN Manager UNICORN also automatically opens the Method Editor the System Control and the Evaluation modules These modules are minimized until you activate them Log off after you Always log off when you leave the computer to prevent others from accidentally are finished changing or deleting your files or disturbing your method runs There are two ways to log off e Select Tools Logoff or e Click the Logon Logoff icon 03 0014 92 ep20 How to create a method 4 4 How to create a method Introduction Chromatography runs are programmed as Methods in UNICORN Before you can proceed with a chromatography run you need either to use an existing method or create a new method This chapter describes two different ways to create new methods In this chapter This chapter contains the sections below Topic How to use the Method Wizard How to use text instructions ep2l 4 How to create a method 4 1 How to use the Method Wizard 4 1 Introduction Are wizards al ways available How does the wizard work How to create a new method 03 0014 92 ep22 How to use the Method Wizard This section describes how to use the Method Wizard to create a new method For most purposes customized methods can be created simply by setting appropriate values for the method variables Wizards are available for AKTAdesign systems delivered with standard strategie
26. nd monitor the separation processes online through method based or manual control To evaluate and present stored results from separation processes Note All modules are active when the program is operating and are not closed when they are minimized A minimized System Control unit may control a process ep5 1 Introducing UNICORN 1 1 About UNICORN Work flow The work flow in UNICORN can be divided into four distinct stages Each stage is described in separate chapters in this manual The flow chart below shows the work flow stages 1 Create a method 2 Run the method EN Evaluate the results 4 Compile a report Help functions An online help utility is included in the UNICORN software The table below describes how to access the help utility If you want to access Then the general help utility open the Help menu in any of the software modules context specific help e click the Help button in the dialog box topics or e press the F1 key on your keyboard Security The table below describes the main security functions in UNICORN Feature Function Access Security Only authorized users can access UNICORN Each user is assigned an access level which defines the func tions that the user is permitted to use Connection Security A running system can only be con trolled from one connection Systems may be locked with a password to prevent other un authorized use
27. o apply a Select a layout from the Saved layouts list in the Layout library Click the Apply saved layout selected layout button e p45 7 How to view results 7 2 How to view the run documentation 7 2 How to view the run documentation Introduction The full documentation of a method run is stored in the result file You can view this information in the Documentation dialog box in the Evaluation module This section gives an example of how to view the Logbook How to open the Maximize the Evaluation module and either run documenta tion e Select the View Documentation menu command or e Click the View Documentation icon E The documenta This is an illustration of the Documentation dialog box with the Logbook tab selected tion window Click the tabs to view other information Documentation x Varisbles Scouting TexMethod Notes Questions Columns Reference Curves Evaluation Procedures Method Information ResultInformation Stat Protocol Settings Caliration Logbook EyelustionLog Result Name 0 00 ml Method Run 2001 03 19 07 33 58 Method id 48Quantitate Result v Niklas Uh ES E 0 00 ml Base CV 0 98 mi Mono S HR 5 5 IF Alam Waining fe Method 7 Manual End Block r Block Column Pressure Limk v Eros Base SameAsMan 7 System EE aoaie Enabled 500MPa 0 00MPa lock Start Instiuctions ase SameAsM ai gee od Base Wavelength 280 nm OFF OF
28. o your system documentation for the correct procedure You initiate the method run in a series of dialog boxes in the Run Setup in the System Control module The Start Protocol for the method decides which pages you need to fill in The steps in the table below is an example of a Run Setup sequence When you are finished in one dialog box you click Next to proceed Step Action e Select the File Run menu command or e click the Run toolbar icon Run The Run Setup opens Select a method and click OK If your method run involves a fraction collector i e Frac 950 the first step is usually the setup dialog box See How to set up Frac 950 below The Variables dialog box opens next Verify and fine tune your method before you proceed In the Notes dialog box some information can already be present Click the Start Notes tab and add your own comments How to set up Frac 950 How to name the result file How to perform method runs 5 Action In the Questions dialog box some questions may be mandatory and must be answered before you can start In Evaluation Procedures you select the automated operations you want the system to perform after the UNICORN run Select Print Chromatogram for an automatic print out after the run 8 The Method Information page is a summary of information about the run Click Next to proceed to name your result file and define where it should be stored In the Frac
29. on Once you have a defined method in place you can perform a method run You can monitor its progress in the System Control module This chapter describes how to perform the method run the data display how you can focus on the information you are interested in and make adjustments while the method run is in progress In this chapter This chapter contains the sections below Topic How to run your method The System Control module How to change the way your run is displayed Manual control ep29 5 How to perform method runs 5 1 How to run your method 5 1 Introduction Connect to the system Prepare the system How to start a method run 03 0014 92 e p30 How to run your method Follow the instructions in this section if you want to start a separation run based on the method you created and saved in the previous chapter Also use this procedure if you want to run other methods Before you can start a run you must connect to the system Open the System Control window and look at the Connection panel in the Run Data section If you are not connected the panel will show the text NO Once you are connected the text changes to YES Refer to the User Reference Manual if your system is not connected N Hold Pause Continue Run method Connection Status Instruments After the system is connected it must be prepared Verify that this already has been done or refer t
30. ons The table below describes how to open the Properties dialog box to edit the way the panes are displayed Step Action 1 Right click in the pane you want to edit 2 Select Properties from the menu 3 Click the tab for the pane you want to edit Result The page shows all the options that you can select in this particular pane You also have a choice of different styles colors and axis layouts for your curves Yass Curve StyleandCokur Flow Schema Logbook FunDataGous RunDataCoou Ome x t wiInsiruments You can select which curve the Y axis scale refers to in two different ways e Click the curve name at the top of the Curve Data pane or e Click the Y axis scale to toggle between the curve scales The table below describes how to set a fixed value range for the Y axis for a selected curve in the Properties dialog box Step Action 1 Click the Y axis tab ep35 5 How to perform method runs 5 3 How to change the way your run is displayed Type a minimum and a maximum value The maximum range values allowed are shown above the entry boxes Click OK 03 0014 92 ep36 How to perform method runs 5 5 4 Manual control Introduction In some applications you may want to change some parameters manually during a run This section exemplifies how you can change the pump flow manually How to change The table below describes how to change the pump flo
31. r This chapter contains e An overview of the UNICORN system and general information that you need before you can start e Information about the user documentation for UNICORN and how to use it e A Quick Start Guide that can be used as a shortcut for experienced users that want to start right away In this chapter This chapter contains these sections Topic About UNICORN About this manual About the UNICORN user documentation Quick Start Guide See 1 4 ep3 1 Introducing UNICORN 1 1 About UNICORN 1 1 Introduction What is UNICORN Operating environ ment Windows func tions Compatible chro matography sys tems 03 0014 92 ep4 About UNICORN This section is a general overview of the UNICORN system UNICORN is a complete package for control and supervision of chromatography systems It consists of control software and a controller card for interfacing the controlling PC to the chromatography liquid handling module Liquid chromatography is used in separation processes for analytical purposes or in the biochemical process industry UNICORN runs on a PC under Microsoft Windows 2000 or Microsoft Windows XP It is designed to run under English keyboard settings Note Microsoft and Windows are registered trademarks of the Microsoft Corporation in the United States and or other countries Most Windows functions are also available in UNICORN including e
32. radient page The Gradient tab shows the method graphically 100 120 140 160 180 200 22020 The length of each block is marked at the bottom of the graph e Click the x axis to view the method in time volume or column volumes CV The Start Protocol The Start Protocol tab shows the items that will be displayed as dialog boxes in the Run Setup sequence e Click the checkboxes for the items that you want to select Allruns ep25 4 How to create a method 4 1 How to use the Method Wizard How to save your Before you can run your method you must save it method f e Select File Save or e Click the Save icon 03 0014 92 ep26 4 2 Introduction The Text Instruc tions Editor When do I use Text Instructions Valid instructions How to open the Editor How to create a method 4 How to use text instructions Sometimes you need more advanced editing facilities which are available when you work directly in the Text Instructions Editor in the Method Editor This section is a brief description of this process You can use the Text Instructions in the Method Editor to build your method step by step You can also use the editor to modify instructions in methods created by wizards or based on templates Use text instructions when you want to e change selected instructions in the method e g the outlet valve position e add blocks or instructions e g Watch instructions
33. rs from changing parameters 03 0014 92 ep6 Introducing UNICORN 1 Feature Function Data Security Result files from an ongoing separa tion run can be saved automatically at preset intervals to minimize data loss if the system fails The results are saved locally if the network communic ation fails Electronic Signatures Method and result files can be signed electronically for enhanced security and accountability ep7 1 Introducing UNICORN 1 2 About this manual 1 2 About this manual Introduction This section provides a general description of the manual the contents and the pre requisites for the examples and instructions that are presented in the Getting Started guide The purpose of The purpose of Getting Started is to present a quick and easy guide to the system Getting Started for a user with limited or no experience of UNICORN The work flow is presented in the form of practical instructions for how to operate a model system These instructions form a basic framework that the reader can expand on by reading selected parts in the other manuals The model system UNICORN software can be used in numerous possible system variations For practical reasons the user documentation is based on a model system that consists of e AKTAexplorer 100 e Strategy E100F400 e Frac 950 Note If you use another system you may find that the descriptions and instructions do not match your system o
34. s If your system is a process systems please refer to the system documentation The Method Wizard consists of a number of dialog boxes in which you answer questions and receive instructions on how to create your method The options in subsequent boxes depend on the choices you have made in the initial dialog box The table below describes an example of how to create a method with the Method Wizard Action Click the Method Wizard icon in the Method Editor module gt ne AN Result The Method Wizard dialog box appears Note If several systems are available you must first select which system you want to use How to create a method 4 Step Action Select the appropriate parameter values and click Next Method Wizard for System Explorer Demo 3 X Main Selection Column and Buffe Prep Main Selection Anion_Exchange Column Any Column Position Posilionl Bypass I Flexible Flow Rates NOTE Different Flow Rates are Set on Variable Page IT Flow Regulation of the System Pump I BulterPrep X lt Back Next gt Frnsh Cancel Help Set Defaut Note Click Set Default to restore all settings to the default values On each new dialog page select the appropriate parameter values and click Next to continue Click Finish in the last window Result Run Setup opens ep23 4 How to create a method 4 1 How to use the Method Wizard The Run Setup The Ru
35. t the same way that you save a completed run Run Hold Continue End Ls 03 0014 92 ep38 Scouting 6 6 Scouting Introduction You can use Scouting to automatically repeat a series of method runs with a systematic variation of one or more parameters This chapter gives an example of how to use Scouting In this chapter This chapter contains the section below Topic See How to set up a Scouting Scheme 6 1 e p39 6 Scouting 6 1 Howto set up a Scouting Scheme 6 1 Introduction When to use Scouting Before you start Example Flow Rate Scouting 03 0014 92 e p40 How to set up a Scouting Scheme When you prepare your method you can define a number of variables in the method for Scouting The definition of how the series of method runs will be performed is called a Scouting Scheme You can use Scouting for example e to screen for the best column to find the optimal pH to test column capacity sample volume to find the optimal flow rate for binding and elution to optimize gradient length and slope Scouting Schemes are defined in the Run Setup in the Method Editor Before you can start you need to create a new method for example by using a method wizard You can also add a Scouting Scheme to an existing method Note Variables must be defined for Scouting The table below describes how to set up a Scouting Scheme Step 7 Action Click the Scoutin
36. t the system This chapter describes how to start the program and log on as a user This chapter contains the section below Topic See How to start UNICORN and log on 34 How to start your system 3 3 1 How to start UNICORN and log on Introduction This section describes how to start the UNICORN program and how to log on as a user oe and The system administrator creates and defines users and may also create your initial passwor password You may change the initial password to another later The program can also be set up so you can logon without a password How to start the Note If UNICORN is already started by a previous user proceed to How to log program on There are two ways to start the program If you start with then a UNICORN icon on double click the icon your desktop Ab a7 ONIG ORN the Windows Start locate the program under Programs Unicorn and click menu UNICORN ae Logon dialog This is an illustration of the UNICORN Logon dialog box ox ae UNICORN logon User name ee ae Password Cancel Help Note The Logon dialog has a Password text box only if a password is required ep19 3 How to start your system 3 1 How to start UNICORN and log on How to log on The table below describes how to log on to UNICORN The four program The program has four windows or modules When you start the program and log windows o
37. tem that it is set up to operate The system specific part is usually referred to as the Strategy The Strategy defines available method and manual instructions system settings run data curves and method templates UNICORN gt al System Note The examples in this guide are generally based on the E100F400 strategy Templates are basic methods that can be used as a starting point for developing customized methods The method variables in a suitable Template is adjusted to create a method for another application Values at breakpoints in the Method and instruction parameters may be defined as Variables Variables makes it easy to adapt a method to a particular chromatography run e A framework Method with default parameters can be changed to create variants e A Method can be used in automatic Method Scouting where one or more parameter Variables are changed systematically Systems settings or method instructions specify acceptable limits for monitor signals during a separation run A Warning dialog box may be displayed on the screen if a specified limit is exceeded The system will still continue to run after a Warning Note Refer to the User Reference Manual for information about Warnings The topic is not covered in this manual epl7 3 How to start your system Introduction In this chapter 03 0014 92 ep18 How to start your system The first step when you begin your work with UNICORN is to star
38. to evaluate and save the res ults 10 How to create and print reports Menu commands field names and other text items from the software are quoted exactly as they appear on the screen in a bold typeface e Example Run Setup Search paths are shown in a bold typeface with a separating colon between each level e Example View Windows Customise i e the menu command Customise in the sub menu Windows from the View menu Text entries that UNICORN generates or that the user must type are represented by a monotype typeface e Example Connection change The following pre requisites must be fulfilled before you can use this manual the way it was intended e You need to have a general understanding of how your PC and Windows works In most cases universal computer functions will not be explained e UNICORN must be installed and configured correctly on your computer e You need to understand the concepts of liquid chromatography Terminology and functionalities will be explained only when they differ from normal practise e Before you try to operate a chromatography system based on the instructions in this manual you need to study and understand the safety information that is part of the system documentation epg 1 Introducing UNICORN 1 3 About the UNICORN user documentation 1 3 About the UNICORN user documentation Introduction The user documentation for UNICORN is divided into three separate manuals
39. tore in new chromatogram Compare Cancel Help The table below describes how to import curves and store them in a new chromatogram Step Action 1 Define search criteria for the folder result chromatogram and or curve name Use the Browse command buttons e p47 7 How to view results 7 3 How to compare curves Step 7 Action Click Search Result A list of found curves is displayed Repeat step 1 2 to add more curves to the list e Select the individual curves that you want to import or e Click Select all to import all curves Click the checkbox Store in new chromatogram and enter a name in the text box Compare is default Note You can also save the curves in the active chromatogram Select one of the Curve options Overlay Stack or Mirror Result This will decide how the curves are displayed Click OK How to set the stack offset Shift Curves by Offset Offset Una ma r Selected curves will ba included 1401 UVI_215nm 102 UV2_230nm 215 UVI_215nm If you selected the Stack option the Shift Curves by Offset dialog box is opened automatically Adjust the offset distance between the curves to a suitable value 03 0014 92 ep 48 How to print your chromatograms 8 8 How to print your chromatograms Introduction This chapter describes how to print open chromatograms Proceed to 10 How to create and print reports on pag
40. trol module You have a choice of four different panes that can be open one at a time or all at once in separate parts of the window The table below describes how to select the panes that are displayed in the System Control module Step Action e Select the View Panes menu command or e Click the Customise Panes icon Customize Panes x MRundata nnn WiCurves MFlow Scheme MiLogbook Cancel Help 3 Click OK The Run Data pane shows the current values for the running parameters When the system is running the text Run is displayed in the Run Status panel If the system is operated manually the text Manual is displayed The illustration below shows only part of the Run Data pane ep 33 5 How to perform method runs 5 2 The System Control module The Curve Data pane The Flow Scheme pane The Logbook pane How to view a single pane 03 0014 92 ep 34 The Curve Data pane shows selected monitor signals as curves during your method run All curves are stored in the result file Normally the curves are scaled with auto scaling i e the scale is adjusted continually to the highest and lowest values for each curve ray URS Cond 1 Cond Cons Con pt Presse Iw Curves Tere Sempres SangieFlow Anini Aunt ADS 300 ao 100 0 100 mo O O op 02 LAI of 0 10 12 14 16 18 20 22 24 26 28 30 32 as 3 30 46 The Flo
41. ual and in the other UNICORN manuals General concepts and common chromatography terminology are not explained here In this chapter This chapter contains this section Topic See Concept definitions 2 1 03 0014 92 ep14 2 1 Introduction Alarms Batch run BufferPrep Chromatogram Curves UNICORN concepts 2 Concept definitions e This section contains explanations and definitions of a number of UNICORN concepts that are used in this manual e The concepts are sorted in alphabetical order Note The section also lists some concepts that are described only in the User Reference Manual These concepts are included for reference since they may be found in menus and dialogs that you will use while working with this manual Systems settings or method instructions specify acceptable limits for monitor signals during a separation run An Alarm dialog box will be displayed on the screen and an optional alarm can sound if a specified limit is exceeded The system will be paused Note Refer to the User Reference Manual for information about Alarms The topic is not covered in this manual You can perform a Batch run of a number of result files in the Evaluation module The files do not have to be open and the run operates in the background The procedure is useful if you want to print a number of results with the same settings or if you want to perform integration with the same parameter settings o
42. ueues are used to link several methods together on the same or on different systems Example A MethodQueue can be set up to conduct a CIP study of a number of columns through a controlled series of scouting runs Note A method can be placed in a MethodQueue if the system is busy when the operator wants to run the method Refer to the User Reference Manual for information about MethodQueues The topic is not covered in this manual The Method Wizard is a user friendly tool to create new methods The Wizard takes the user step by step through the creation process Method Wizards are supplied with UNICORN installations for AKTA design systems UNICORN creates Result files when a method is run The Result files contain e Run data from the monitors in the chromatography system Example UV absorbance flow rate conductivity etc e Documentation from the run Example Logbook entries calibration settings scouting parameters text method etc e Saved results from evaluations of the run data Example Peak integrations simulated peak fractionations etc Scouting is used to repeat a series of Method runs automatically with predetermined changes in the values for one or more Variables A Scouting Scheme is defined as part of the method Scouting is used for optimizing chromatographic processes Strategy Template Variable Warnings UNICORN concepts 2 Part of the UNICORN software is specific for the sys
43. user manual chromatography software Getting Started With O UNICORN 5 0 Amersham in 03 0014 92 o e Biosciences Getting Started with UNICORN 5 0 03 0014 92 Edition AB 2004 02 Office addresses Amersham Biosciences AB SE 751 84 Uppsala Sweden Amersham Biosciences UK Limited Amersham Place Little Chalfont Buckinghamshire England HP7 9NA Amersham Biosciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 USA Amersham Biosciences Europe GmbH Munzinger Strasse 9 D 79111 Freiburg Germany Amersham Biosciences K K Sanken Building 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Amersham Biosciences China Limited 13 F Tower I Ever Gain Plaza 88 Container Port Road Kwai Chung New Territories Hong Kong www amershambiosciences com Trademarks UNICORN Drop Design BioProcess KTA and AKT Aexplorer are trademarks of Amersham Biosciences Limited Amersham and Amersham Biosciences are trademarks of Amersham plc Microsoft and Windows are trademarks of the Microsoft Corporation in the United States and or other countries Terms and Condition of Sale Unless otherwise agreed in writing all goods and services are sold subject to the terms and conditions of the company within the Amersham Biosciences group which supplies them A copy of these terms and conditions is available on request Any use of this software is subject to Amersham Biosciences Standard Software End User Lic
44. ves may not be needed anymore e Select Edit Delete Curves to remove all unwanted curves Note The original curves that were created during the run can never be deleted You can either save your results in the original file or in a new result file If you want to save then in the original result e choose File Save file or e click the Save icon in a new result file e choose File Save as How to create and print reports 10 10 How to create and print reports Introduction The Evaluation module provides extensive tools to create detailed reports This chapter describes how to create reports In this chapter This chapter contains the sections below Topic See How to print an existing report format 10 1 How to edit an existing report format 10 2 How to create and print a customized report format 10 3 e p 57 10 Howto create and print reports 10 1 How to print an existing report format 10 1 How to print an existing report format Introduction This section describes how to use an existing report format to print a basic report with a chromatogram and text How to printare The table below describes how to print a report port Step Action e Choose File Report or e Click the Report icon E Result The Generate Report dialog box opens Select format Global Chromatogram Result This creates a report containing the chromatogram and the answ
45. w the pump flow Step 1 Action Select Manual Pump Result the System Pump Instructions dialog box opens The Pump radio button is selected Click the function you want to change i e Flow Enter a new value FlowRate under Parameters You can use the ar rows to step the value up or down e Click Execute to execute the instruction immediately or e Click Insert to add the instruction to the list below the instructions menu Note If there are instructions on the list the Execute button will execute all instructions on the list at the same time Click Close to close the dialog box The System Pump The illustration below shows the System Pump Instructions dialog box Instructions box Demo system Pump Instructions x Instructions 1 m Parameters G Pump FlowRate 0 00 100 00 men C Flowpalh BulterPrep pH 10 00 H mimin paze c Sampleflom S50 Alums Mon DirectLoad_960 Executen C Free SystemPumpControlMode k SystemPiD Parameters Close C Other SamplePumpConiroMode 960 IT Auto update lf this is checked the parameter fields wil be updated during method run Note The parameter values will be updated continually during the run if the Auto update checkbox is selected e p37 5 How to perform method runs 5 4 Manual control How to end your Click the End button to end the method run before it is finished You can save the run manually partial resul
46. w Scheme pane shows a schematic view of your system configuration The Logbook pane is shown at the bottom The Logbook shows exactly when the instructions in the method were executed during the run It also shows all manual instructions that were performed and all alarms and warnings that were registered 0 00 min Method Run 3 26 2002 47 16 AM Method sdtest Result v Wkios idies001 res 0 00 min Batch ID 4E9620F 4 3604 11D6 A4C46 00D08726BCC0 0 00 min Base CV 0 10 mi 0 05 min Block Flow rata 0 05 min Base SomeAsMain 0 05 min Flow 1 00 ml min 0 05 min End Block 0 05 min Block Column Pressure Limit 0 05 min Base SameAsMain 0 05 min Alarm Pressure Enabled 400MPa 000MPa 0 05 min End Block 0 05 min Block Stert_Instructions 0 05 min Base SameAsMain 0 05 min Wovelength 280 nm OFF OFF 0 05 min AvorsgngTimelUV 2 56 sec 0 05 min End Block 0 05 min Block Butteevalve Al Inlet If you want then to enlarge a pane right click and select Maximize to return to the origin right click and select Restore al size 9 3 Introduction How to edit the pane displays How to change the Y axis scale display How to set fixed values for the Y axis How to perform method runs 5 How to change the way your run is displayed There are a number of ways to change the way your method run data is displayed in the System Control module This section describes a few of the opti
47. x Step Action Click the Y axis tab Click the curve you want to edit Click the Fixed radio button Enter new minimum and maximum values Repeat steps 2 to 4 for all other curves you want to edit Click OK to execute the changes and close the window You can select which curve the Y axis scale refers to in two different ways e Click the curve name at the top of the Curve Data window Q r e Click the Y axis scale to toggle between the curve scales The table below describes how you can zoom in on a curve to view more details Step Action Place your cursor in a corner of the area you want to enlarge Press and hold the left mouse button and drag diagonally over the area Result A rectangle appears Release the mouse button Result The area within the rectangle is enlarged How to view results 7 Step Action 4 Repeat steps 1 3 to enlarge the selected area further 5 Right click and select Undo Zoom to zoom out one step 6 Right click and select Reset Zoom to return to the full window view How to save a You can save your edited layout It can be applied later to any result file Follow layout the steps in the table below Step Action 1 Right click and select Properties to open the Chromatogram layout dialog box 2 Select the Layout library tab 3 Click the Save current layout as button 4 Enter a name for the layout and click OK How t
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