E-Man Hybrid - Mettler Toledo
Contents
1. aspirate dispense blowout return have ta occurred in the current pipetting ses IT sion CYCLES and the count number are shown in the display More detailed descriptions of the modes and cycle counter are included in the OPERATION section E Man Hybrid options are available by pressing a recessed button with the end of a dis posable tip see Options page 10 Sleep Mode 3 minutes of no activity the display will go to sleep to con serve battery life To operate the pipette again simply touch any key or press the plunger button through the 1ST STOP E Man Hybrid remembers its display mode the state of the cycle counter and the set volume from its last use E Man Hybrid will also wake up when the volume lock is moved to the unlocked position without pressing the plunger to the 1ST STOP c If the volume lock was UNLOCKED when Hybrid went to sleep changes the upper set volume will not be registered until either the plunger button is pressed through the 1ST STOP and allowed to return to the set position or the volume lock is moved to the locked position Volume Lock The volume lock lever prevents the set volume drifting during operation When locked the plunger button cannot be turned Move the lever to the right to free the plunger button noting that UNLOCKED is shown flashing on the display This is a warn ing to the user to LOCK E Man Hybrid before pipetting If pipet ting2. if the bat tery is now removed the total cycle count will change to 10 496 when the battery is reinserted the next lowest multiple of 256 and counting will restart at 10 497 Leave Total Cycles Count and scroll to User Calibration by pressing the MODE key Pipette Techniques Alert Because E Man Hybrid includes position sensing technology and an LCD it can provide helpful information when technique errors such as an insufficient pause at the PICKUP position are sensed In the event of such an alert the LCD displays bAd indicating the type of alert Turn the Alert on or off by pressing the CC key when in Alert option With Alert turned on Technique Error during Pipetting LCD shows Insufficient pause at Pickup point bAd Pickup started away from Pickup point bAd PIC Plunger is not returned to set volume bAd PIC Dispense stroke not completed bAd dSP Pipetting Technique Alert works in forward mode only and not in TRACK or TITRATE modes Leave the Alert option and scroll to GLP Service option by pressing the MODE key You also leave Options altogether by pressing the plunger button or moving the volume lock to the unlocked position and back 11 User Calibration E Man Hybrid allows the user to place multiple offsets into the calibration curve as opposed to a single offset as in other man ual pipettes User Calibation allows corrections at up to 50 points on the curve and can be turned off and on
3. properly on the shaft with minimal force do not use more force than is required Tip Immersion Depth The recommended depth for tip insertion into the sample for each E Man Hybrid model is shown below E Man Hybrid Volume Range Immersion Depth 2 uL 01 2 1 2 mm 10 uL 0 5 10 uL 1 2 mm 20 uL 2 20 uL 2 3 100 uL 10 100 uL 2 3mm 200 uL 20 200 uL 3 6mm 300 uL 30 300 uL 3 6mm 1000 uL 100 1000 uL 3 6mm 2000 uL 200 2000 uL 3 6mm Tip immersion depth is critical If these depths are exceeded ihe volume measured may be inaccurate possibly out of spec ification Tip angle is also important Hold the pipette within 20 degrees of vertical Operation This section is divided into three sub sections relating to the three display modes PIPET TRACK and TITRATE and a sec tion on the CC option Some information in the PIPET mode section will also apply to the other two modes PIPET Mode Before pipetting valuable samples it is good idea to practice aspirating and dispensing water before pipetting with actual samples 1 Unlock the volume lock and set the desired volume as described on page 4 Move the volume lock lever back to the locked position to begin measurements When locked ihe volume display will not change just like in a standard pipette 2 Attach a new RAININ tip by pressing the shaft into the tip with only sufficient force to make a good seal 3 Press the plunger button to the first sto
4. shaft if necessary If the shaft was dropped remove it to see if the piston is bent If so return the instrument for service Worn seal and or o ring All models up to 2000 pL incor porate a polyethylene seal and o ring Examine the seal and o ring replacing them as necessary Pull off the old seal and o ring position the new seal and o ring on the pis fon assembly as shown in the drawings on pages 18 19 and reassemble the pipette Note It is NOT necessary to recalibrate E Man Hybrid after changing seals o rings or shafts Do not lubricate any components E Man Hybrid uses a dry sealing system 15 Service Calibration and Repair RAININ Pipette Repair and Calibration facilities It is recommended to use only genuine RAININ replacement parts such as seals o rings and shafts It is NOT necessary to recalibrate the pipette affer changing the seal o ring or shaft Recalibration of the pipette is only necessary when the piston is replaced and should be done only by qualified factory trained personnel in one of the above mentioned facilities For pipettes under warranty please note that the warranty will be voided if the pipette has been dam aged as a result of physical or chemical abuse or if the pipette has been repaired or recalibrated by any service facility which is not authorized by Rainin In the US call 800 543 4030 for service Or visit the web WWW rainin com service asp Service is also availab
5. www mt com rainin Mettler Toledo Schweiz GmbH Im Langacher 8606 Greifensee CH Verkauf 044 944 45 45 salesRN ch mt com Service 044 944 47 47 serviceRN ch mt com Mettler Toledo GmbH Ockerweg 3 35396 Giessen DE Verkauf 49 641 507 222 info mtd mt com Service 49 641 507 307 info mtd mt com Mettler Toledo SAS 18 20 avenue de la P pini re 78220 Viroflay FR Ventes 0130971717 marcom fr mt com Laboratoire d Etalonnage Accr dit 264 rue Abraham Lincoln Service Pipette 62400 Bethune FR Service 03 21 64 54 66 marcom fr mt com Mettler Toledo 5 Leuvensesteenweg 384 1932 Zaventem BE Sales 32 2334 0211 general mtb mt com Service 32 2334 0211 general mtb mt com Mettler Toledo 5 Miguel Hern ndez 69 71 08908 L Hospitalet de Llobregat Barcelona ES Ventas 0034 93 223 7666 mtemkt mt com Servicio T cnico 0034 93 223 7666 mtemkt mt com Mettler Toledo A S Naverland 8 2600 Glostrup DK Salg 43 27 08 25 info mtdk mt com Service 43 27 08 25 info mtdk mt com Rainin Instrument LLC 7500 Edgewater Drive Box 2160 Oakland CA 94621 0060 METTLER TOLEDO Company Prices and specifications are subject to change without notice Copyright 2008 Rainin Instrument LLC 9920 348
6. E 6202 076 6202 077 6202 078 6202 079 6202 080 6202 427 6202 081 6202 082 6202 424 GLASS X non LTS E 6202 183 6202 184 6202 185 6202 079 6202 080 6202 427 6200 131 6200 138 6200 143 6200 150 6200 154 6200 415 6200 161 6200 6 6200 132 6200 139 6200 170 6200 151 6200 155 6200 414 6200 162 6200 H 6202 083 6202 083 6202 083 6202 084 6202 084 6202 084 J 6200 196 6200 196 6200 198 6200 201 6200 200 6200 416 6204 083 6204 083 6204 083 6204 083 6204 083 6204 083 6204 083 6204 083 L 6204 084 6204 084 6204 084 6204 084 6204 084 6204 084 6204 084 6204 084 A PLUNGER BUTTON SHAFT COUPLING SHAFT TIP EJECTOR PISTON ASSY SEAL PISTON O RING STRONG SPRING SEAL RETAINER BATTERY DOOR 6200 160 6200 6202 074 6200 6200 163 6200 69 68 68 6202 081 6202 082 66 67 BATTERY BATTERY TRAY 1 SLIDE THE BATTERY INTO THE BATTERY TRAY POSITIVE SIGN DOWN lt 2 SLIDE THE ASSEMBLY 2 INTO THE OPENING ul THE HANDLE INSERT BATTERY FULLY IN TRAY SO THAT POSITIVE SIGN ON BATTERY A AND POSITIVE SIGN ON TRAY B ARE BOTH SHOWING SLIDE TRAY INTO HANDLE Figure 5 Replacing the Battery o 7 E Man Hybrid 2 amp 10 uL E Man Hybrid 20 uL J F 76 D D E Man Hybrid 100 uL E Man Hybr
7. RAININ Pipetting 360 E Man Hybrid Manual pipette with real time display Continuously gt Adjustable gt I Microliter ba Pipette 7 with LCD volume ranges from to 2000 uL E Man Hybrid 200 uL shown Table of Contents Introduction 25 n cued 1 Battery Life oes Shen 2 Display Modes and Cycle 3 Sleep Mode es oe hasta eee or 3 Volume LOCK rema re Nother 4 Setting VOIUMC etes Ses 4 Tip Selection and Mounting 5 Tip Immersion 5 Operation oie s Cen ERU C AUD ee 5 PIPET MOQO he dehet ec 5 TRACK Mode 6 TITRATE Mode 8 Cycle Counter 9 edet XR Rd de V PUE 9 Service Reminder 10 Total Cycles Count 11 Pipette Technique 11 User Calibration Pario RIT 12 Standard Pipetting Guidelines 13 oc 13 Reverse Mode Pipetting 14 Temperature Considerations 14 Aultoclaving 333 tite sente Lach oes 14 Tip Ejector Arm Removal 14 Troubleshooting and Repairs 15 Servi
8. as desired for different samples This manual describes setting a single point calibration offset Setting a Single point Calibration Offset Suppose you wish to offset the pipette to compensate for a solution with higher density than water Repeated weighings indicate that the volume setting on a 20 uL pipette needs to be offset by 1 microliter Set E Man Hybrid to the desired volume to be offset in this case 20 pL Press the Options key with the end of a tip Scroll though the MODES until UCAL is shown on the display CUCAL is shown when there are no existing user calibration settings If user calibration has already been set the display will read UCAL on or UCAL oFF 1 Press the MODE key to set user calibra tion The symbol for SET will flash on and off LI 2 Press the CC key to toggle between Inc increase and dEc decrease To x Increase the offset the display appears as shown here with a set volume of 20 uL U CAL also shows in the display 3 Now use the MODE key to scroll through the increasing values The display will u ca flash while you are increasing values inn Set the actual volume dispensed in this case 19 0 and press the Options key with the tip end to enter the value 4 When the offset is entered donE appears on the display briefly then the pipette is returned to the previous display mode If the plunger is moved during any of the above step
9. ble tip on the pipette shaft 2 Press the plunger button fully to the 2ND STOP 3 Immerse the tip in liquid and allow the button 10 return slow ly to the fully up position Wait a moment for the liquid col umn to reach equilibrium in the tip 4 Wipe any excess liquid from the outside of the tip without touching the orifice 5 To dispense rest the end of the tip against the vessel wall and press the plunger to the 1ST STOP Hold this position a few seconds or long enough for the liquid column to reach equilibrium again 6 Remove the tip from the receiving vessel without blowing out the remaining liquid 7 Return excess sample in the tip to the original sample con tainer by blowing it out if desired Discard the used tip Temperature Considerations Warm or cold liquids can be measured with good precision by using a consistent pipetting rhythm This will help minimize any differences in heating or cooling effects within the pipette Use a new tip each time for best accuracy and precision when measuring samples with temperatures greatly different from ambient and do not pre rinse You will get best results if there is no delay between picking up the sample and dispensing it Equilibrate pipette tips and liquid to the same temperature before pipetting Autoclaving Autoclavable parts of E Man Hybrid are the shaft shaft cou pling and the tip ejector 121 C 1 bar 15 20 minutes dry cycle 110 C for 10 minutes Do not au
10. ce Calibration and Repair 16 Replacement Parts 17 Line 05 18 19 Specifications ceo ee e e ERR S 20 E MAN SOAGE Eon e t ec 20 EC Declaration of Conformity 21 Contacting RAININ Back Page Figures Figure 1 E Man Hybrid Pipette 1 Figure 2 Hybrid Controls and Indicators 2 Figure3 Volume 0 4 Figure 4 Removing the Tip Ejector Arm 14 Figure 5 Replacing the Battery 17 Line Drawings E Man Hybrid 2 300 pL 18 E Man Hybrid 1000 uL and 2000 pL 19 Contents of Package E Man Hybrid as ordered Sample tips Instruction Manual Rainin Test Report Performance Assurance Brochure If any item is missing or call Customer Service 800 472 4646 RAININ and LTS are registered trademarks and E Man is a trademark of Rainin Instrument LLC E Man Hybrid Pipette U S Patent Nos 5 614 153 5 849 248 Other patents pending LTS Tip Shaft System For use under U S Patent Nos 6 168 761 1 6 171 553 1 6 568 288 B2 6 737 023 1 6 745 636 B2 6 967 004 B2 Introduction Unique position sensing technology accurately monitors the piston position at all times during operation and in real time updates the LCD liquid crystal display The pisto
11. d if not installed and used properly i e in strict accordance with the instruction manual may cause harmful interference to radio communications It has been tested and found to comply with the limits for a Class A com puting device pursuant fo Subpart J of Part 15 of FCC rules which are designed fo provide reasonable protection against such interference when operated in a commercial environment Operation of this equipment in a residential area is likely to cause inter ference in which case the user at his own expense will be required fo take whatever measures may be required to correct the interference LIMITED WARRANTY See the Limited Warranty and Limitations of Liability Statement Please complete and return the Warranty Registration Card on receipt of your pipette RAININ pipettes are calibrated with RAININ fips To assure excellent repro ducibility and performance use only RAININ tips as recommended in this manual Specified performance is guaranteed only when RAININ tips are used Contacting RAININ Technical Information T 800 543 4030 F 510 564 1617 Pipette Service T 800 662 7027 F 781 935 7631 service rainin com Direct Order Line T 800 472 4646 F 510 564 1617 RAININ website www rainin com From outside North America T 1 510 564 1600 global rainin com from outside the U S METTLER TOLEDO Offices METTLER TOLEDO website
12. e stroke Stop at the second stop Release the plunger and discard the used tip Multidispense In TRACK mode E Man Hybrid allows you to accurately dispense several known volumes from a single pickup When multidispensing you will need to calculate the total volume of sample for the separate aliquots you need PLUS an extra residual volume to ensure accuracy in the last aliquot For example if you want to dispense 6 aliquots of 25 yl each you should pick up 6 x 25 150 ul 15 ul Total 165 ul Set a desired maximum volume including the extra residu al volume as described above and attach a RAININ tip as described earlier Press the plunger to the first stop and hold there The set volume and PICKUP will show on the LCD eq 2 Place the tip into the sample and relax thumb pressure on the plunger The piston will move upward aspirating the sample As the tip fills the LCD shows the volume in the tip in real time increasing as you release pressure on the plunger 3 When you have aspirated all the sample into the tip hold your thumb steady to stop aspiration Withdraw the tip slowly from the sample 4 Move the tip end to the first of the receiving wells and dis pense the desired aliquot volume in the example 25 uL reading the display Stop when the LCD shows you have dispensed the first aliquot and move to the next well 5 Continue in this fashion until all the aliquots have been dis pensed Then discard t
13. eagent as desired by pressing the plunger very slowly until you reach the titration point 0 pH change recording the volume displayed on the LCD at that point 5 Discard the remainder and eject the used tip CC Cycle Counter E Man Hybrid s cycle counter records the number of complete pipetting cycles which have occured since the last time the count was zeroed Maximum is 999 cycles then the count rolls over to O 1 Press the CC key CYCLES and the number of complete cycles are shown in the display as shown here If this is the first time CC key is pressed or if the counter has been reset the number reads O 2 With CC engaged E Man Hybrid will record and count each complete pipetting cycle press the plunger to the 15 STOP aspirate sample dispense sample press through blowout cycle count added e release plunger The counter will not record incomplete cycles mixing 3 To switch off the cycle counter press CC key again This does not clear the count but removes it from the display 4 To clear the count and reset to zero a with CYCLES showing press and hold the CC key The CYCLES display will momentarily go blank then return with the cycle count reset to zero b with CYCLES already off Press and hold the CC key until the count resets fo zero Note If the battery is removed when there is existing cycle count data the counter is reset 10 the last stored multip
14. ed with the level of care appropriate for laboratory instrumentation Three types of hanger are available to hold your E Man Hybrid conveniently when not in use CR 7 Free standing carousel holds seven pipettes HU M3 Set of three individual magnetic Hang Ups for mounting on ferrous surfaces HU S3 Three Hang Ups attached to a clamp which fits onto a shelf 20 EC Declaration of Conformity according to ISO IEC Guide 22 and EN45014 Manufacturers Name Rainin Instrument LLC Manufacturer s Address 7500 Edgewater Drive Oakland CA 94621 USA declares that the following product Product Name E Man Hybrid Microliter Pipette Model Number E Man Hybrid conforms to the following Product Specifications Safety EN 61010 1 2001 2nd Edition EMC FCC Part 15 Class A EN 61326 04 1997 Al 06 1998 A2 2001 A3 2003 Emissions EN55011 1998 A1 1999 CISPR 11 2003 Edition 4 1 2004 Class A Class A Radiated Emissions EN 61326 04 1997 Al 06 1998 A2 2001 A3 2003 Immunity IEC 61000 4 2 Edition 1 2 2001 Electrostatic Discharge Immunity Test 61000 4 3 2002 Radiated Radio Frequency Electromagnetic Field Immunity Test Supplementary Information Responsible Signatory Jim Petrek VP R amp D Date August 2007 This Declaration of Conformity applies only to products which have the CE mark attached WARNING This equipment generates and uses radio frequency energy an
15. f four service intervals The display counts down the number of days left before the next scheduled service Service interval LCD Counts down from 1 year 364 days 364 6 months 182 days GLP2 182 4 months 121 days GLP3 121 3 months 91 days GLP4 91 Off GLPO Bi pu The display alongside shows a pipette uL with a 3 month service interval selected 40 and 90 days remaining before the next scheduled service When the next service is due within 30 days a message is dis played each time the pipette wakes from sleep mode The mes sage flashes CAL dUe followed by in xx dAY xx number of days left a few times before reverting to the normal display 10 When the service is due the display flashes CAL dUE a few times before reverting to the normal display If the service becomes overdue the number of days overdue up to 99 is flashed with a minus sign in front Leave GLP Service interval and scroll to Total Cycles Count by pressing the MODE key Total Cycles Count E Man Hybrid keeps track of the total pipetting cycles since manufacture shown in the LCD If the total cycles count is less than 999 it will show in the bottom right corner For larger numbers the volume display is also used as seen here This example shows a total cycle count of 10 727 If the battery is removed E Man Hybrid remembers the total cycle count in increments of 256 So in this example
16. he extra residual volume which was included 10 ensure the last aliquot would be accurate 6 Discard the used tip TITRATE Mode Note E Mon Hybrid is not intended to replace special ized titration equipment and will give resulfs to an accuracy of about 10 depending on operator skill especially using very small sample volumes Press the MODE key until TITRATE is shown on the LCD In TITRATE mode the display first shows zero as shown in the top image alongside As the plunger is depressed the posi tion sensor communicates to the LCD the piston position or the volume dis pensed from the tip by reference to the Zero volume as a negative value as shown in the lower image Set a desired maximum volume and attach a RAININ tip as described earlier Press the plunger to the first stop and hold there The word TITRATE the set volume and PICKUP will show on the LCD 2 Place the tip into the sample and relax thumb pressure on the plunger The piston will move upward aspirating sam ple into the tip As the tip fills the LCD shows the volume in the tip as a negative number in real time decreasing in value as you release pressure on the plunger When the tip is full the display reads zero as shown in the top image above 3 To begin titration depress the plunger slowly to control the volume dispensed The LCD displays the accurate real time value for the volume dispensed 8 4 Titrate the r
17. id 200 amp 300 uL H F G E Legend for Hybrid 2 uL to 2000 pL Models A Plunger Button B Shaft Coupling C Sha D Tip Ejector E Piston Assembly F Seal G O ring H Stroke Spring J Seal Retainer 18 Hybrid 1000 pL E Man Hybrid 2000 uL 10 Specifications These manufacturer s specifications should be used as guide lines when establishing your own performance specification S pecifications Volume Increment Accuracy Precision Model uL uL 96 pL pL x 2 0 2 0 002 120 0 024 6 0 0 012 1 0 27 0 027 13 0 013 2 0 15 0 030 0 7 0 014 10 pL 1 0 0 01 2 5 0 025 12 0 012 5 0 15 0 075 0 6 0 03 10 0 10 01 0 4 0 04 20 uL 2 0 02 75 0 15 2 0 0 04 10 15 0 15 0 5 0 05 20 10 02 0 3 0 06 100 pL 10 0 1 3 5 0 35 1 0 0 1 50 08 04 0 24 0 12 100 08 0 8 0 15 0 15 200 pL 20 0 2 25 0 5 1 0 0 2 100 08 0 8 0 25 0 25 200 08 16 0 15 0 3 300 pL 30 0 2 25 0 75 1 0 0 3 150 08 12 0 25 0 375 300 0 8 2 4 0 15 0 45 1000 pL 100 1 30 3 0 0 6 0 6 500 0 8 4 0 0 2 1 0 1000 08 8 0 0 15 15 2000 pL 200 2 30 6 0 0 6 1 2 1000 08 8 0 0 2 2 0 2000 0 8 16 0 0 12 24 Specifications are subject fo change without prior notice E Man Storage After use store the pipette in a clean safe place E Man Hybrid is precision instrument and should be treat
18. le of 256 below the actual count when the battery is replaced i e if the cycle count was 245 it is reset to zero If the cycle count was 519 it is reset to 512 For Total Cycles Count since man ufacture see additional options Options E Man Hybrid s options can be accessed by lightly pressing the unmarked OPTIONS button recessed between the MODE and CC keys with ihe end of a tip or a straightened paper clip The OPTIONS key is shown by the arrow When the OPTIONS key is pressed the first of the options is dis played The MODE key is used to scroll through the options and the CC key is used 10 switch options on or off Scrolling with the MODE key the sequence of E Man Hybrid s options is 1 Service Reminder GLPx where x is a number O off or 1 fo 4 service interval no of days before next service Total Cycles Count since manufacture Pipette Techniques Alert ALEr on or off User Calibration UCAL on or off User Calibration settings UCAL SET User Calibration clear UCAL CLr if data exists or 6 Return to previous display mode for normal operation hM GLP Service Reminder Because E Man Hybrid keeps track of time the software can remind the user that GLP calibration service is approaching due or overdue This function is set OFF in new pipettes and is invoked when E Man Hybrid is sent to RAININ Service The ser vice center can set one o
19. le outside the US See the RAININ website home page www rainin com and click on the link Customers outside North America METTLER TOLEDO Pipette Repair and Calibration facilities or authorized RAININ distributors who provide service can be found by following this link and specifying the country or geo graphical region Contact Technical Support at 800 543 4030 for further infor mation Acids and Corrosives After pipetting concentrated acids or highly corrosive solu tions disassemble E Man Hybrid liquid end and inspect and clean the piston assembly shaft and seal with distilled water Dry all components thoroughly and reassemble Extensive contact with corrosive fumes may result in prema ture seal wear and damage to the piston Exposure of inter nal components to corrosive fumes can be reduced by using RAININ tips with aerosol barrier filters 16 Replacement Parts Description 2yl B 6204 111 6204 111 6204 111 6204 111 6204 111 6204 111 10g 20g 100g 200p 500 see pages 18 19 1000 2000 pl 6204 085 6204 086 6204 087 6204 088 6204 089 6204 090 6204 091 6204 092 A 6204 144 6204 145 6204 146 6204 147 6204 148 6204 149 6204 150 6204 6204 111 5 C 6202 063 6202 064 6202 065 6202 066 6202 067 6202 425 6202 068 6202 214 C 6200 134 6200 140 6200 145 6200 147 6200 157 6200 413 D 6202 071 6202 071 6202 071 6202 073 6202 073 6200 419 D 6200 133 6200 133 6200 144 6200 148 6200 156 6200 419
20. lent to the volume of sample present in the tip Sea LLLI ul TRACK mode enables functionality previously unavailable on manual pipettes such as measuring an unknown sample vol ume dilution and multidispensing Note Accuracy and precision results for specialized operations such as Measuring Dilute amp Multidispense will depend greatly on the skills of the operator espe cially when very small sample volumes are involved Measuring an unknown volume 1 Set a desired maximum volume and attach RAININ tip as described earlier Press the plunger to the first stop and hold there The set volume and PICKUP will show on the LCD Place the tip into the sample and relax thumb pressure on the plunger The piston will move upward aspirating sam ple As the tip fills the LCD shows the volume in the tip in real time increasing as you release pressure on the plunger When you have measured the unknown volume hold your thumb steady to stop aspiration Read the volume on the LCD Withdraw the tip slowly from the sample 6 5 Dispense as desired Touch the tip end against the side wall of the receiving vessel and press the plunger slowly past the first stop PICKUP will appear on the LCD as you reach the first stop As the piston is depressed beyond the FIRST STOP position the display reads bLo to indicate the Blowout part of the stroke Stop at the second stop Release the plunge
21. n location relative to zero position describes the volume of liquid in the tip Position sensing provides certain advantages to the pipette user Functions that were previously only possible using an electronic pipette can be performed such as titration dilution multidispensing and measuring unknown volumes E Man Hybrid also has the unique ability fo warn of technique errors for example if the aspiration rate is too fast this can be alerted to the operator Scheduled GLP service reminders can be displayed and the user can program multiple offsets into the calibration curve Real time sensing allows true pipetting cycles to be counted Turning the plunger button is easy as there are no geared dials with mechanical resistance and the display is always visible from the normal operating position LCD shows volumes o button modes and other info volume adjustment Volume lock 77 Tip ejector button Fingerhook Keypad see figure 2 Body Comfortable ergonomic design Constructed from high impact 2 Quick release ejector arm polymer e j Stainless steel tip ejector arm Shaft Disposable tip Figure 1 Hybrid Pipette LCD MODE Key CC Key Plastic v Strip Plunger Button Volume Battery Lock beneath display 7 Tip Ejector Button Recessed Key for Additional Options 4 2 Hyb
22. p The word PICKUP is shown on the display as an aid to locate the HOME position Hold the plunger but ton in this position 5 Holding the pipette vertically place the tip into the sample to the proper depth p 5 and relax thumb pressure on the plunger The light piston spring moves the piston upward aspirating sample Do not release the plunger button or the piston may snap up resulting in inaccurate measurement Pause briefly with the tip end still in the sample to ensure that the full volume of sample is drawn into the tip Withdraw the tip from the sample If any liquid remains on the outside of the tip wipe it carefully with a lint free tissue taking care not to touch the tip orifice Dispensing 1 Touch the tip end against the side wall of the receiving ves sel and press the plunger slowly past the first stop PICKUP will appear on the LCD as you reach the 1ST STOP to blowout bottom of stroke Wait 1 second for volumes to 300 uL 1 2 seconds for 1000 uL and larger Still holding the plunger withdraw the tip sliding it along the wall of the vessel Release the plunger and press the tip ejector button lightly to discard the tip Use a new tip for each sample to prevent carry over Repeat for the next pipetting cycle TRACK Mode Press the MODE key until TRACK is shown on the LCD In TRACK mode the piston position relative to the zero point is displayed in real time equiva
23. r and discard the used tip Dilution 1 7 Set a desired maximum volume and attach a RAININ tip as described earlier Press the plunger to the 1ST STOP and hold there The set volume and PICKUP will show on the LCD Place the tip into the diluent and relax thumb pressure on the plunger The piston will move upward aspirating dilu ent As the tip fills the LCD shows the real time volume in the tip increasing as you release pressure on the plunger When you have aspirated the diluent into the tip hold your thumb steady to stop aspiration Observe the digital display and record the volume Withdraw the tip slowly from the sample With the pipette tip clear of the diluent release the thumb pressure slightly to aspirate a small volume of air to create an air gap Record the new volume including the air gap Place the tip end into the sample and slightly release the thumb pressure to aspirate then stop when you have aspi rated the desired sample volume Dispense the sample air gap and diluent all at once touch ing the tip end against the side wall of the receiving vessel and pressing the plunger slowly past the first stop PICKUP will appear on the LCD as you reach the first stop Or if you wish place the tip in the liquid and move the plunger up and down several times to mix in the tip As the piston is depressed beyond the FIRST STOP position the display reads bLo to indicate the Blowout part of th
24. reen at right reading U CAL 2 Press and hold the CC key for a long press if you wish to clear the offset The display shows YES Note the CC key must be held for a long press to clear the offset When done the display shows U CAL Clrd briefly before reverting to the previ ous display mode Es pas 1 U CAL I7 1 U CAL 73 n gt Lt Standard Pipetting Guidelines E Man Hybrid pipettes incorporate several new features which enhance pipetting consistency You should also maintain Consistent pickup and dispense rhythm Consistent speed and smoothness when pipetting Consistent pressure on the plunger button at the first stop Consistent immersion depth See table on page 5 Pipette vertically or within 20 of vertical Don t invert or lay E Man Hybrid flat with liquid in the tip aOoarwn Pre Rinsing Some solutions may leave a film on the inside tip wall This film remains relatively constant in successive pipettings with the same tip so excellent precision can be obtained by refilling the tip and using the refilled volume as the sample Successive samples from this same tip will exhibit good reproducibility 13 Reverse Mode Pipetting In reverse mode pipetting the operating sequence is reversed TRACK mode will not show true volume picked up 1 Mount a disposa
25. rid Controls and Indicators E Man Hybrid has three display modes PIPET TRACK TITRATE and a cycle counter CC described on the following pages Options Pipette Technique Alert GLP Service Reminder and User Calibration are described later in this manual E Man Hybrid is a comfortable rugged pipette with built in ergonomic features such as light plunger and tip ejection forces a tip ejector shock absorber and a comfortable con toured handle with fingerhook The pipette will give many years of service with proper minimum care and maintenance Battery Life Power is supplied to the LCD by a 3v lithium watch type battery in a sliding tray beneath the display E Man Hybrid is shipped with a plastic strip isolating the battery terminal switching power off For operation remove this strip by pulling straight out from the battery tray TITRATE TRACK The LCD briefly shows all segments then U CAL UNLOCKED the pipette software revision then PrES H H H H appears Remove this message by PICKUP pressing the plunger through the 1ST eS STOP When the battery voltage goes belowa T predetermined threshold the battery ri symbol turns on and flashes at a slow rate This indicates the battery will need be changed within a week or so The battery will give up to two years service before needing replacement See page 17 for Battery replacement diagram Operation is not affec
26. s the calibration procedure is aborted and the pipette returns fo normal operation 5 Once set the display shows the offset value instead of the original set value U CAL is shown with uL flashing as reminders that User Calibration has been switched on 6 This single point offset of 1 uL is carried through the volume range of the pipette The above procedure simply adds a single offset More points can be added 10 get better calibration over the entire range of the pipette 12 Turning Calibration on off The User Calibration offset can be turned on and off as desired Simply go to the Options menu by pressing the Options key with the end of a tip Then scroll through the Options using the MODE key When you reach User Calibration toggle this func tion on or off with the CC key If User Calibration is on and you have set an offset value you will see the display set volume change after you switch User Calibration off Setting a New Offset or Clearing an Existing Offset Go fo the Options menu by pressing the Options key with the end of a tip Then scroll through the Options using the MODE key With the display reading UCAL To set a new offset Press the MODE key The SET symbol will flash and you should proceed as described in steps 1 4 on the previous page To dear an existing offset 1 Press the MODE key until UCAL Clr shows Then press the CC key The pipette displays the confirmation sc
27. ted while the battery symbol is flashing If the battery is not changed eventually the display reads dEAd and then goes blank Although the pipette will operate with a dead battery the volume aspirated will be unknown Display Modes and Cycle Count Select display modes by pressing the MODE key sequentially The CC key switches the Cycle Counter on or off PIPET MODE Operation is exactly the same as in other manual pipettes When setting volume with the vol ume lock set to the unlocked position the display shows the changing value When the volume lock is moved to the locked position the set volume is shown When the piston is at the 1ST STOP position the display reads PICKUP as shown TRACK MODE TRACK is shown in TRACK the display The volume of sample in the tip is shown real time Lt m um m QUO cma m m 20 o A c v As the piston is depressed beyond the IST STOP position the display reads bLo to indicate the Blowout part of the stroke TITRATE MODE TITRATE is shown in the display The display shows the volume of sample dispensed from the tip in real time as a negative number TITRATE relative to the upper set volume when the display shows 0 0 h CYCLE COUNTER The CC key turns on or off the cycle counter and clears the count Cycle count is a visual ref erence of how many true pipetting a r D cycles depress plunger
28. toclave the complete pipette or any parts other than listed here Tip Ejector Arm Removal Press in the quick release tabs on the ejector arm and slide the ejector down To replace the ejector arm insert the shaft through the large opening align the top with the tip ejector pushrod and push until the ejector arm snaps in place Figure 4 Removing the Tip Ejector Arm 14 Troubleshooting and Repairs Warning When removing the shaft from the pipette body make sure the spring seal and o ring do not fall off the piston especially on the smaller models Sample Splash liquid inside the mechanism 1 Remove the tip ejector arm Also refer to the appropriate drawing on page 18 or 19 On pipettes up to 1000 uL unscrew the shaft coupling B and remove the shaft C For 2000 pL unscrew the shaft Inspect the seal assembly and piston for contamination The piston should be shiny and free of corrosion Clean with distilled water or isopropyl alcohol Dry with a lint free tis sue and reassemble affer inspecting the interior of the shaft for any contamination If piston corrosion or staining is evident do not use the instrument In the US call 800 543 4030 for Technical Support In other countries please use the number on the back page Leaks Inaccuracy Abnormal Stroke 1 2 Loose shaft Tighten coupling by hand Split or cracked shaft Remove the tip ejector and inspect the shaft Replace the
29. while unlocked after two cycles LOCh Err appears VOLUME LOCK LEVER LOCKED UNLOCKED Figure 3 Volume Lock Setting Volume 1 Turn the volume lock lever right as shown above so the vol ume setting mechanism is unlocked and free to turn 2 Looking at the volume setting on the LCD rotate the plunger button to change volume counter clockwise to increase and clockwise to decrease volume The volume display changes as you move the plunger button Although not nec essary for E Man Hybrid for consistency with other manual pipettes with mechanical volume setting it is a good idea always to dial down to set the desired volume 3 Stop when you reach the desired volume and move the vol ume lock back to the locked position so E Man Hybrid is ready for pipetting Volume ranges and increments are shown below E Man Hybrid Adjustable Range uL Increment Model Range Recommended pL 2 uL 0102 0 1102 0 002 10 uL Oto 10 0 5 to 10 0 01 20 uL 0 to 20 2 to 20 0 02 100 uL 0 to 100 10 to 100 0 1 200 uL 0 to 200 20 to 200 0 2 300 uL 010 300 30 to 300 0 2 10001 Oto 1 000 100 to 1000 1 20001 0102 000 200 to 2000 2 4 Tip Selection and Mounting Always use RAININ tips with E Man Hybrid pipettes the pipettes are calibrated with RAININ tips and performance to published specifications can only be guaranteed with RAININ tips To mount a tip press E Man Hybrid shaft into the end of the tip The tip will seal
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