User Bulletin ABI PRISM
Contents
1. 4 Fill the syringe slightly more than desired volume for example if you need 0 5 mL fill to 0 7 mL 5 Raise the syringe to eye level and hold the syringe tip up with graduation scale outlined against a white background Tap the glass barrel with your fingernail to raise air bubbles and then gently push them out using the plunger 6 Move the syringe plunger to the desired volume Blot the syringe tip with a lint free lab tissue without drawing any liquid out of the tip 8 Hold the syringe by the stainless steel hub and gently screw the filled syringe into the gel block finger tight only CAUTION Do not screw syringe into the gel block by holding the glass barrel as this will break the glass barrel Hold only the stainless steel hub Syringe Care and Maintenance Page 3 of 6 Cleaning the Syringe Cleaning Syringes Thoroughly clean syringes before loading new polymer Page 4 of 6 To clean the syringe Step Action 1 Remove the plunger by slowly drawing it from the glass barrel count slowly to 5 i e one thousand one one thousand two while keeping the entire syringe submerged in water IMPORTANT Moving the dry plunger quickly can damage it resulting in premature failure or leakage around the plunger 2 Remove the ferrule from the syringe a Soak the ferrule in warm not boiling water for as long as it takes to remove crystals if any in the ferrul2. User Bulletin ABI PRISM 310 Genetic Analyzer October 26 1999 updated 11 2000 SUBJECT Syringe Care and Maintenance Overview This user bulletin describes the care use and maintenance of the syringes used with the ABI PRISM 310 Genetic Analyzer This information applies to the syringes manufactured by the Kloehn company Following the simple care use and maintenance tips in this bulletin will ensure maximum syringe life and satisfactory results with normal use We recommend storing this user bulletin in the ABI PRISM 310 Genetic Analyzer User s Manual P N 903565 Warranty Each syringe is manufactured to exact tolerances and is tested with deionized Information water Warranty replacement policy states that syringes will be replaced free of charge if a problem occurs within the first 3 months 90 days of use The lifetime of each syringe varies with use and care In This User Bulletin This user bulletin includes the following topics Topic See page Safety 2 Syringe Components Liquid Transfer Technique Cleaning the Syringe a AJ w w Handling and Storage i Applied A Biosystems Safety Documentation User Five user attention words appear in the text of all Applied Biosystems user Attention Words documentation Each word implies a particular level of observation or action as follows Note This word is used to call attention to information IMPORTANT This word call
3. ation for life scientists Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses P N 4314273B Stock No 106UB12 02
4. e b Rinse the ferrule with deionized water 3 Clean the glass barrel with warm water Dissolve all crystals if there are any 4 Rinse the glass barrel with distilled deionized water 5 Inspect the O ring in the stainless steel hub of the syringe for damage and replace it if necessary IMPORTANT Make sure the O ring does not block the hole in the stainless steel hub 6 Inspect the O ring on the ferrule and replace it if necessary 7 Place the ferrule back onto the syringe When the syringe is clean do one of the following If Then the syring is to be used insert the plunger into the barrel count slowly to 5 using deionzed water as a lubricant the syringe is to be stored a dry the plunger and barrel b Insert the plunger into the barrel count slowly to 5 c Store as a matched set Syringe Care and Maintenance Handling and Storage Matched Sets Each syringe is shipped with the plunger installed in the barrel as a matched set to ensure that the syringes Recommendations Do not leak Have the proper breakaway force Note Breakaway force is the pounds of normal force not psi needed to start the plunger in motion Interchanging plungers and barrels can result in Leakage Shortened syringe life breakaway force too high or too low Capillaries not filled completely breakaway force too high Keep these important facts in mind Syringe Care and Mai
5. ntenance Do not place O rings on the syringe plunger Doing so will alter the syringe breakaway force and cause improper filling of capillaries Do not rapidly heat or cool an assembled syringe as doing so will crack the glass barrel Allow the polymer to reach room temperature before filling the syringe Remove the plunger from the barrel slowly count slowly to 5 Moving the dry plunger quickly in the barrel may damage the Teflon plunger and cause leakage around the plunger Resting the plunger on the lab bench may change the shape of the Teflon tip Do not store the syringe barrel and plunger separately Do store syringes Dry Away from direct sunlight With the plunger in the barrel Do not lubricate the plunger with polymer before inserting it into the barrel Lubricating with polymer can damage the Teflon plunger Use only deionized water to lubricate the syringe Page 5 of 6 Copyright 2000 Applied Biosystems For Research Use Only Not for use in diagnostic procedures ABI PRISM and its design and Applied Biosystems are registered trademarks of Applera Corporation or its subsidiaries in the U S and certain other countries ABI and POP 6 polymer are trademarks of Applera Corporation or its subsidiaries in the U S and certain other countries All other trademarks are the sole property of their respective owners Applera Corporation is committed to providing the world s leading technology and inform
6. s attention to information that is necessary for correct operation of the kit or instrument CAUTION This word informs the user that damage to the instrument could occur if the user does not comply with the information It also indicates a potentially hazardous situation that could result in minor or moderate injury to the user WARNING This word informs the user that serious physical injury or illness to the user or other persons could occur if these required precautions are not taken DANGER Indicates an imminently hazardous situation that if not avoided will result in death or serious injury General Warning WARNING CHEMICAL HAZARD Some chemicals used with Applied Biosystems instruments or kits may be hazardous and can cause injury illness or death Check regularly for chemical leaks and chemical spills which represent potential safety hazards to the operator If a leak or a spill occurs follow clean up instructions in the Material Safety Data Sheets MSDSs or in the Waste Profile in the Site Preparation and Safety Guide Hazardous Chemical Warnings are prominently displayed on the labels of all hazardous materials Always read the appropriate MSDSs and Waste Profiles before using the chemicals in any way User Safety Always wear the appropriate protective gloves clothing and eyewear when handling Precautions chemicals Ordering MSDSs_ Material Safety Data Sheets MSDSs for hazardous chemicals manufactured by Applied Bios
7. ystems will accompany your first shipment To receive additional copies of MSDSs at no extra cost call Applied Biosystems at 800 327 3002 or call your local Applied Biosystems service representative For chemicals required for this instrument but not manufactured or sold by Applied Biosystems please obtain the MSDSs from their manufacturers Page 2 of 6 Syringe Care and Maintenance Syringe Components The Basic The elements of the syringe discussed in this user bulletin are shown below Components Ferrule O rings Stainless steel hub Teflontip Glass barrel Plunger Plunger button if 05 06 07 03 09 10m Peab OPO dhhil GR1686 Liquid Transfer Technique Filling Syringes WARNING CHEMICAL HAZARD POP 6 polymer may be a potential mutagen It can cause irritation to the skin eyes and respiratory tract Read the MSDS in the Site Preparation and Safety Guide Wear appropriate protective eyewear clothing and gloves To fill a syringe Step Action 1 Grasp the syringe by the glass barrel and plunger button 2 Slowly draw 1 0 mL of deionized water into the syringe and then dispense the water completely into a waste container 3 Slowly draw 0 15 mL of polymer into the syringe Raise to eye level and while holding syringe tip up dispense completely into a lint free lab tissue This ensures that the deionized water does not dilute the polymer and if done correctly eliminates bubbles from the syringe
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