HLA Fusiontm IVD and Research Software v4.xx
Contents
1. Use Attach database to link to any HLA Fusion mdf database file to which you are not currently linked If you are using Detach and Attach database to move a database then once you have detached the database and relocated it you must attach it again NOTE The database you are attaching must reside on the selected server Why am I getting an error message during a database backup that desktop is nota valid destination If your logon settings for the FUSION instance of SQL Server are not set for logging on as a local system account then you will not be allowed to back up the database to your desktop Do the following to change your log on settings Close the Fusion Database Utility Select Start gt Control Panel Double click Administrative Tools Double click Services Right click on the item SQL Server FUSION and select Properties Click the Log On tab shown below and make sure it has the following settings red highlight Exit out of the SQL Server FUSION Properties dialog box and open the Fusion Database Utility You can now store a database backup on a local destination NOOR ON gt SQL Server FUSION Properties Local Computer General Log On Recovery Dependencies Log on as 6 Local System account V Allow service to interact with desktop Help me configure user account log on options Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 10 One Lambda Inc A Therm2. AB DR TurnaroundTime 14 21 or 14AB 21DR DCN example line 1 12 0287 7867 8 AB DR 14AB 21DR 074 SDF format Each field is separated by commas This file lists in this order BoxSlot DonarlD SampleType AB DR or AB DR TurnaroundTime 14 21 or 14AB 21DR DonarCenter example line 1120287 7867 8AB DR14 21074 Back to the Top HLAF MAN Vv4 x x EN 00 Rev 0 HLA Fusion FAQ 26 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top Local Sample Patient ID Data This file is in the form of a Microsoft Excel spreadsheet This file lists in this order Row 1 Column Title Local and Sample and Patient Column A LocallD Column B SamplelIDName required Column C PatientIDName Example Back to the Top ONOMAR WHT A B amp Local Sample Patient local sample1 patient local2 sample2 patient2 local3 sample3__patient3 local4 sample4 patient4 local5 samples patient5 local6 isample6__ patient6 local sample patient locals samples patient8 HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 27 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top Reports Why can t view my HLA Fusion Reports To view reports your computer must have some form of printer driver installed If you do not have a printer driver installed you can download a free copy of PDF Distiller from Adobe com or Mi
3. Print Geaph 227 6 37 01 637 M 6 4101 641 M B 4501645 B27 M 8 28 01828 M B44 02Be4 M BONBA Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 18 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top What are the different kinds of graphs available in Ab Tracking e Line Time Scale e Column Sample e Column Specificity e Sample Dilution e LABScreen Mixed Ratio graph Timescale Can save my Ab Tracking configuration and how Yes The user selection for the samples that are selected for a graph plot can be saved by clicking on the Save button on the top section next to the Donor PRA button From 47282015 v To amp 6201S v Find Formula Baseline Donor PRA C save MFI 9 Data Table Sample Sample Dilute Seconda hciude ni paar Sample ID Facto Ab Yy PC NC Final Assignment Archived Y Sessid fv 0602 2015 1 P06 0261426F 11831 76 47 57 Negative Vj 06092015 2 R01 0328728Y 17247 77 110 63 Da2 A 05312015 3 P11 9044124L 10843 96 33 77 0Q2 007 008 00 Note Any time the user selects a particular patient the samples that were previously part of the save operation will be graphed automatically How can I view only Donor Specific Antigens on the graph The Track DSA checkbox allows the user to plot only Donor Specific Antigens on the graph Can the layout and columns displayed be saved Yes The field chooser on the top left of
4. Rev 0 HLA Fusion FAQ One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Does the SQL Server authenticate using the application login user name and password Do users need to have db_owner role on the SQL Server to access and use HLA Fusion Do need to have Write and Modify permissions in HLA Fusion sub directories How do bring back the Welcome screen in HLA Fusion software How do add new users to HLA Fusion Why can t access certain HLA Fusion menus and functions What determines the default date range in the HLA Fusion system Which characters should not be used in file names in HLA Fusion Why aren t all the characters typed recognized in a patient sample ID search Reference Files How do update NMDP Serological Equivalence and Catalog Reference Files How often should update NMDP Serological Equivalence and Catalog Reference Files Does HLA Fusion accept cat and cry catalog reference files Why aren t the catalogs just downloaded displaying on the product home page How does HLA Fusion determine the most recent available catalog Why didn t a column in my allele frequency file get imported Why did my auto update fail Data Exporter What does the Data Exporter function do What kind of data can be exported What data formats are supported How does Fusion export data and what is a Data Export Template How do I create modify and delete Data Export Templates How do use a Data
5. avoid using all special characters such as those listed in the following table in all naming of files for HLA Fusion including filenames session sample names or database names The following may not be a complete list We recommend the use of alphanumeric characters only Character Name comma semi colon colon period slash backslash question mark percent sign asterisk vertical bar quotation mark apostrophe single quotation mark lt less than gt greater than dash hyphen Why aren t all the characters typed recognized in a patient sample ID search We continue to enhance the HLA Fusion software to fully support regional settings and characters However if you come across a regional character that is not supported please let us know so that it can be considered for an enhancement in future releases Back to the Top HLAF MAN Vv4 x x EN 00 Rev 0 HLA Fusion FAQ One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top Reference Files How do update NMDP Serological Equivalence and Catalog Reference Files These files can be updated in one of two ways e From the HLA Fusion or product home page click the Download link e Select HLA Fusion menu option Utilities gt Update Reference gt Update Reference File Select the type of reference file you want to update and the means for
6. gradually getting slower and how can speed it up The SQL databases that Fusion uses to store its data become disorganized over time causing analysis to slow down By periodically optimizing the database you can repair errors compact wasted space and optimize data storage Open the HLA Fusion Database Utility 1 Optimize Database from the Database Tasks Menu 2 Select the Database you want to optimize from the Database Name drop down list 3 Click the Optimize button 4 When the database organization is complete you ll see a message indicating successful completion Back to the Top HLAF MAN V4 x x EN 00 Rev 0 HLA Fusion FAQ 11 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top Login amp Administration Can get help with HLA Fusion within the application itself The online help can be accessed from anywhere within the HLA Fusion application when you press the F1 key This provides information and instructions for using HLA Fusion Updates are made to the online help between releases of HLA Fusion so it is considered the most current form of documentation What do do if forget my user name or password Click on the Forgot User Name or Forgot Password link on the HLA Fusion login screen and answer the security questions to retrieve your user name and or password Does the SQL Server authenticate using the application login user name and password No the SQL Se
7. is empty the entire column is not imported into Fusion regardless of any other data it contains If columns are duplicated Fusion gives you an error message and does not import the allele frequency file Back to the Top HLAF MAN V4 x x EN 00 Rev 0 HLA Fusion FAQ 15 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top Why did my auto update fail Please note that proxy enabled networks are not currently supported for auto updates If you receive a message that your auto update did not succeed it could be that the network is busy Please wait a short while and try the update operation again Data Exporter What does the Data Exporter function do The Data Exporter feature allows you to save currently processed patient data to external data files instead of traditional reports This is useful when it is desirable to analyze Fusion data at external agencies such as hospitals and laboratories or where different types of reporting are done such as analysis with a spreadsheet program like Microsoft Excel for instance What kind of data can be exported Fusion allows you to export records of blood samples test sessions and generalized patient donor data What data formats are supported Fusion allows you to export data in three common data exchange formats e CSV Comma Separated Values This format is often used with Microsoft Excel e XML eXtensible Markup Language e TXT tex
8. lt The Following tas are for PlateDesign gt lt add key LABXpressLogPath value C OLI FUSION LABXpress gt lt add key XRCWD value True gt lt add ke lateDirection value vertical gt lt add_kev RATCH SessionName value SName gt Why doesn t my computer recognize the barcode on Molecular Antibody Custom reports For Molecular Custom or Antibody Custom reports you must make sure the Free 3 of 9 Extended font is installed on your computer otherwise the barcode will not be recognized If needed you can download this font for free at http www free barcode font com Can create new custom reports from the analysis screens You cannot create new custom reports from the analysis windows The only custom reports available from the analysis window are ones you previously created through the Reports window Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 29
9. tasks regularly updated by Microsoft NET is used by many Windows applications that take advantage of its prebuilt functionality HLA Fusion is one of them How to install it HLA Fusion software requires NET version 4 5 included in the HLA Fusion installation package Most of the Microsoft operating systems come with the NET framework preinstalled The HLA Fusion software will detect and install the NET framework if needed during installation Do need to uninstall previous versions of HLA Fusion before install the latest version You do not have to uninstall previous versions of HLA Fusion before a new version installation Separate folders will be created during installation to allow easy identification of the different HLA Fusion versions you have installed on your system or network Back to the Top HLAF MAN Vv4 x x EN 00 Rev 0 HLA Fusion FAQ One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top Fusion Database When would you want to detach a Database If you wish to move a database file to another location for disk space considerations or other reasons you must first detach it from HLA Fusion Then you can relocate it and link attach to its new location You may also simply no longer want to have HLA Fusion Software linked to a particular database if the database is no longer current or relevant but you do not want to delete Why would you need to attach a database
10. Export Template to save data What are the metadata options and what can do with them Ab Tracking Can export the Ab graph and how What are the different kinds of graphs available in Ab Tracking Can save my Ab Tracking configuration and how How can view only Donor Specific Antigens on the graph HLAF MAN Vv4 x x EN 00 Rev 0 HLA Fusion FAQ One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Can the layout and columns displayed be saved How can we add donors to a patient from the Ab Tracking module What does matched mismatched Ag mean What does Ab Tracking Threshold do What does the LABScreen Overlay do How can use the LABScreen Overlay UNOS CPRA Calculated PRA What is the UNOS CPRA How can get the UNOS CPRA value for my current patient s within Fusion How can I get the UNOS CPRA value using the Web Version Does the Fusion software automatically update the UNOS CPRA value every time update patient assignments Analysis What things should do first before analyze a session or sample in HLA Fusion Why aren t the CSV files imported displayed on the session import list anymore Why isn t the check box for applying w632 normalization displaying during import of LABScreen w632 CSV files IVD Only How do import HD files How do create a QC Panel IVD Only Why can t I select any sample configuration settings for Demographic Information Why do get an error that s
11. One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x WS ONE LAMBDA ThermoFisher A Thermo Fisher Scientific Brand SCIENTIFIC Frequently Asked Questions FAQs HLA Fusion IVD and Research Software v4 x x Click on a question below to view a topic or answer xPONENT Software What setup parameters are required on xPONENT software to function with HLA Fusion Are there any procedural differences involved with xPONENT software versions Installation Minimum Software Required Minimum Hardware Required SQL Express Databases Do Need to Install the SQL Server Database Myself Non USA Operating Environments Program Setup Options Server Client or Standalone Database Installation What is the NET Framework and is it necessary Do need to uninstall previous versions of HLA Fusion before install the latest version Fusion Database When would you want to detach a Database Why would you need to attach a database Why am getting an error message during a database backup that desktop is not a valid destination How do backup and restore my data How often should back up my data How do migrate the database from SQLExpress to SQL 2008 Server Why is my Fusion software gradually getting slower and how can speed it up Login amp Administration Can get help with HLA Fusion within the application itself What do do if forget my user name or password HLAF MAN Vv4 x x EN 00
12. You need to download and import the latest Serology Equivalent file from the One Lambda website You can update this file through HLA Fusion using the Download link on any of the home pages or the Utilities gt Update Reference gt Update Reference File menu option Why do get an error message when try to import an output file The csv file you are attempting to import is open or is being accessed by someone else Why is the Date field yellow on the Session Import window HLA Fusion converts Luminex generated CSV file data such as date and time to the local regional code if a regional code is specified in the CSV file A regional code cannot be specified for CSV files created with Luminex software version 2 2 or earlier If the first date field is highlighted yellow it indicates a regional code mismatch In this case it is recommended that you use the drop down selector in the second date field to choose the appropriate date taking into consideration regional date format differences Do archived sessions or samples appear on the Manage Data screen Yes If you do a search on the Manage Data window archived sessions and samples are displayed Does the software allow me to delete LABType IVD Only or Micro SSP data at the sample level Yes If you have permission to delete data you can use the Manage Data window to delete LABType or Micro SSP samples NOTE You cannot delete combined samples or sessions containing combined sampl
13. cientific Brand HLA Fusion Version 4 x x Back to the Top How do add new users to HLA Fusion 1 From the HLA Fusion main menu select Profile gt List User 2 Click Add User to add a new user 3 Enter new user information Check the Active box to activate the user account NOTE Supervisors can also choose whether to give certain users the privilege to update reference files and or manage data in the HLA Fusion system Why can t access certain HLA Fusion menus and functions Some functions and menus in HLA Fusion require the user to be supervisor or administrative level This can also be the result of not importing the necessary input files e g demographics or disabling a product you are trying to access If you feel you should have increased privileges within HLA Fusion you must have your supervisor modify your access rights through your User Profile What determines the default date range in the HLA Fusion system The date range set in the Find window in the Session Date field is used as the default date range throughout the software such as in the Navigator and Reports windows Each time you change it and click Find the default changes for the rest of the application Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 13 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top Which characters should not be used in file names in HLA Fusion You should
14. crosoft Office Document Image Writer from Microsoft com In addition you can print and export these reports from the analysis or batch summary window Why did receive a module timed out message when tried to load samples for reporting Check your date range settings through the Find or the Reports window If you have set a lengthy date range the system may time out due to an attempt to retrieve a large amount of data Do archived sessions or samples appear on the Reports window No Archived sessions and samples are not displayed on the Reports window You will need to make these active again before they can be selected for inclusion in a report Do samples excluded from analysis appear on the Reports window No Excluded samples are not displayed on the Reports window Samples must be included in analysis to be included in reports Why is my data missing from the sessions samples list in the Reports module The data you are seeking is probably not within the two week window of time that is currently selected in the Session Date field Revise the date settings to include the timeframe for the data you want to see How are items like sample IDs sorted in reports Sample IDs Patient IDs Well IDs Alleles Serology and so forth are sorted alphanumerically in reports just as they are on other HLA Fusion forms and lists Back to the Top HLAF MAN V4 x x EN 00 Rev 0 HLA Fusion FAQ 28 One Lambda Inc A Thermo Fisher Sc
15. d what can do with them When selecting output fields choose Options above when creating modifying and deleting Data Export Templates a number of special additional options will appear When choosing output fields there is a category called Options at the bottom of the list Fields listed under the Options category can be included in Data Export Templates to change the export format The first of these is labeled Include meta data This option causes the inclusion of a descriptive header to be inserted into the export The header information includes the HLA Fusion software version the user who ran the report the time of report was run the template name used and other useful data You can see that the metadata is very useful for exporting to spreadsheets such as Microsoft Excel The other Options offer additional capabilities mostly having to do with how numbers are displayed in columns Try them Ab Tracking Can export the Ab graph and how Yes The graphs seen during Ab Tracking can be exported to a file during analysis 1 Right click on an Ab Tracking Graph of interest 2 Select Export Graph then click one of the graph types that will appear to the right 3 Two graphs are exported one for each class Auto Scale Set Class I Man Scale Set Class E Min Sale Set LSM Min Scale Set Class I Max Scale Set Class E Max Scale Set LSM Max Scale Set Tine Scale interval Days Displey Sample Oste fot Line Chat
16. es unless you first delete the individual samples that were used to create the combined analysis Can perform auto analysis on import of exon 4 batch sessions No At this time auto analysis of imported exon 4 batch session files is not supported Why can t go back to the session summary screen from the Micro SSP analysis window If you created your Micro SSP session using Manual Entry you cannot return to the session summary screen from the analysis window A simple workaround is to select the recently analyzed data set in the Navigator Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 24 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top My Sero Code is a dash so why is it recorded as a blank in the Patient Results Sero codes that are either a dash or a blank are both recorded as a blank in the Patient Results Assignment for LABType and Micro SSP analysis Why can t remove patient assiqned results from the analysis window You must go to the Manage Patient window to remove the assignment of analysis results to patients Why don t I see the serology value entered for a patient For all manual serology entries you must precede the value with the locus For example if a locus A has a value of 23 you must enter A23 What are the different formats allowed for sample list information The information inside a sample list that you import into HLA Fusion m
17. exes and the output csv file are not separated from each other just as the Luminex software originally generated them How do I create a QC Panel IVD Only QC panels can be created by right clicking a saved LABType session on the Navigator tree and selecting Create Local QC Why can t select any sample configuration settings for Demographic Information If the Demographic Information option is not active in a sample level configuration menu it means you need to import an allele frequency input file Use the menu options Utilities gt Update Reference gt Demographic Allele Frequency Why do I get an error that states Trimmed Mean values are missing and how do resolve it This error occurs when Luminex is run to create a csv data file but the trimmed mean section was not checked before the run was made Run Luminex again making certain to select the option for the trimmed mean section How do archive some of my older data You can choose to archive individual session sample logs by selecting the Data menu selecting the sessions samples you want to archive and clicking the Archive button Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 23 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top What does A 0A 0 mean in LABType and SSP Analysis screens A_0A_OinLABType and SSP Analysis means there is no serology equivalent for the corresponding alleles
18. ientific Brand HLA Fusion Version 4 x x Back to the Top Why is the Report Designer Program in the Reports Module not working You must have Supervisor level privileges to use the Reports Designer In addition please ensure that the Crystal now SAP Report Designer software is installed on your computer If yes please locate the designer executable exe file path and update the HLA Fusion configuration file OneLambda Fusion Interface exe config with the correct pathname To update the configuration file look in the directory C Program Files x86 One Lambda under the directory of the software product you are running i e Fusion Research 4 0 Locate the following line in this configuration file and change the value parameter as follows lt add key ReportDesigner value C Program Files Business Objects Crystal Reports 11 5 crw32 exe gt By default the configuration file is set to work with Crystal Designer version XI Please also refer to the Reports chapter of the HLA Fusion User Manual Here is an example of the line in the configuration file being edited highlighted yellow Key VIEL AMDUGEMA TT Va IUe g gt Jriatorielamvad Com gt key ProductKey value 3DEB63E4 F1DD 40df 86cc F1DASFB6FBF3 gt q1commandTimeout lue 1000 gt eportPath value C OLI FUSION rpt IVD 4 0 gt key DoNotwWarnResolution value gt key Ambiguity_Recommendation value true gt
19. matically change when antigen assignments change You must press the CPRA button to obtain the latest value Analysis What things should I do first before analyze a session or sample in HLA Fusion Verify that you or your supervisor has done the following before analyzing any sessions or samples e Download the latest Nomenclature or Serology Equivalent file before you import catalogs or attempt to analyze sessions samples These files are published every year in January and July of each year The latest versions of these files may be downloaded on the Product Home Page in the upper right hand side of the window under Data and Catalogs e Make sure your collation of SQL Server matches the collation of any client instances If you are not certain please verify the collation with your system database administrator e Make sure you have the latest catalog files as well as NMDP code local code if used or serology equivalent reference files before you analyze You can download or update catalogs from the Download link on the Product Home page e View and modify global product configuration settings before starting analysis Global settings are displayed and be can be modified on the Product Home Page or through the Utilities menu Global settings apply across all newly imported sessions e Save time importing CSV files by verifying that the default URLs and paths are pointing to the locations where these files are commonly stored on you
20. n Mouse or other Windowse compatible pointing device mouse with wheel is required for certain products e A Windowse compatible printer driver PDF Distiller or Microsoft Document Image Writer are available for free The NET framework is Included with the HLA Fusion installation Back to the Top HLAF MAN Vv4 x x EN 00 Rev 0 HLA Fusion FAQ One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top SQL Express Databases HLA Fusion software requires Microsoft SQL Server databases to operate SQL Server Express 2014 is provided with the HLA Fusion Software installation files NOTE The LABXpress module only supports the use of SQL 2008 R2 Do Need to Install the SQL Server Database Myself The HLA Fusion software prompts you to install SQL Server 2014 Express during the installation if there is no FUSION database instance on your computer You can ignore it if you want to install your own copy of SQL Server Non USA Operating Environments English UK German Germany Spanish Spain French France Portuguese Brazil Norwegian Norway Japanese Japan Chinese Taiwan Traditional Chinese China Simplified Turkish Turkey Sweden Sweden Italian Italy Greek Greece Dutch Holland Danish Denmark Note The HLA Fusion application itself is not translated but all operating system messages and labels will be in the language of the specific operating e
21. nvironment Back to the Top HLAF MAN Vv4 x x EN 00 Rev 0 HLA Fusion FAQ One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top Program Setup Options Complete recommended All One Lambda OLI products and the Fusion Database Utility are installed and enabled Custom You select from a list of all the OLI products and the Fusion Database Utility The products you select are the only ones that will be enabled on your system once the HLA Fusion software is installed Server Client or Standalone Database Installation The installation mode you should select depends on the configuration of computers that will be accessing the HLA Fusion database that you are creating or connecting to Client install select if more than one machine will be accessing the database in a network environment but your machine does not actually store the HLA Fusion database You will need to connect to a server and an HLA Fusion database on another machine Server install select if more than one machine will be accessing the HLA Fusion database in a network environment and your machine stores the database Other machines will connect to you and share the database Standalone install select if your machine is the only computer storing and accessing the HLA Fusion database What is the NET Framework and is it necessary The NET Framework is a necessary library of predefined solutions to common computing
22. o Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top How do backup and restore my data Use the following HLA Fusion Database Utility options completing the related fields as necessary Backup Database Makes a copy of the specified database to the local drive of the computer or server Can be set up to run automatically daily weekly or monthly Can only be performed from the computer server on which the database resides Restore Database Can restore a database backup to any existing or new database except for the current database You can replace the current database with a backup database by using the Merge Database option NOTE the backup file used must have a bak file extension How often should back up my data You should back up your database whenever advised to by the HLA Fusion application or documentation In addition you should establish regular and frequent database backups which can be automated through the Backup Database utility The frequency depends on the how often and how much new data is generally added to your HLA Fusion databases base this on how much data you are willing to replace or lose if a database becomes unusable How do migrate the database from SQLExpress to SQL 2008 Server Open the HLA Fusion Database Utility and perform the following database tasks 1 Back up the SQLExpress database 2 Restore the database you backed up on the SQL 2008 server Why is my Fusion software
23. o the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top Installation SYSTEM REQUIREMENTS Minimum Software Required Microsofte Windowse 7 or 8 1 operating system Microsofte NET Framework Version 4 5 Visual JSharp version must match the NET Framework version you are using Microsofte SQL Server 2008 R2 or SQL Server Express 2014 Microsoft Windows XP and SQL Server 2005 are no longer supported Microsoft SQL Server Express 2014 is included in the HLA Fusion installation Please note that Microsoft SQL Server Express 2014 requires Windows 7 Service Pack 1 and NET 3 5 1 on Windows 8 1 Note Before you upgrade to a new Service Pack from a third party vendor such as Microsoft contact your One Lambda representative to verify that HLA Fusion supports it If you are missing some of the Microsoft requirements listed see the Microsoft com Web site Minimum Hardware Required e 1 Ghz Pentium Processor or equivalent e 32 bit x86 or 64 bit x64 microprocessor e 1 GB hard disk space more may be required for large databases Note Regardless of where you install HLA Fusion as much as 400MB of space on your local hard drive may be required for temporary installation files as well as any programs you may need 512MB RAM 8 bit graphics adapter and display for 256 simultaneous colors VGA display with minimum of 1280 x 960 resolutio
24. r system or network These settings can also be modified in the General Configuration section of the Home page e If using xXPONENT Luminex software refer to the xPONENT section above to identify things needed to be done prior to analysis on HLA Fusion NOTE Some of the above tasks require supervisor user privileges You may have to verify with your supervisor that these tasks have been completed Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 22 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top Why aren t the CSV files imported displayed on the session import list anymore Once a CSV file has been imported it no longer displays on the Luminex session import list unless you select the Include Imported CSV check box Why isn t the check box for applying w632 normalization displaying during import of LABScreen w632 CSV files IVD Only Make sure that you have imported LABScreen Class Single Antigen catalogs before you import w632 sessions or samples If you import the catalog s after the import of w632 CSV files then you may have to return to the HLA Fusion Home page and then go back to the LABScreen session summary screen before the w632 normalization check box is displayed How do import HD files If you are importing an HD output file generated from Luminex make sure you select it from an intact Luminex session folder one where run files and their ind
25. ram in the Reports Module not working Why doesn t my computer recognize the barcode on Molecular Antibody Custom reports Can create new custom reports from the analysis screens HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top XxPONENT Software What setup parameters are required on xPONENT software to function with HLA Fusion xPONENT 3 1 cannot convert run and analysis files to CSV for each well in a batch by default nor automatically export to CSV Output files The option to convert and export CSV Output files must be selected under the CSV Options menu Refer to the xPONENT 3 1 Software Manual XxPONENT 3 1 Rev 2 must be manually configured to automatically convert raw run files to CSV format and export results of analysis in CSV format for each well in a batch To turn on this functionality checkboxes must be set on the CSV and Batch Export Options screen The CSV Export Folder and Automatically Exported Batch File locations must also be configured in this screen In xPONENT 4 2 this issue has been fixed in the IXPO 00008103 update downloadable at the Luminex updates website xPONENT 4 2 also requires installation of Microsoft NET version 4 5 to run on Windows This installation should be done by your IT group To produce the CSV Output file statistics required by HLA Fusion it is important to select the Include Advanced Stati
26. reen The Analysis window will appear On the top right of the Analysis screen click the Overlay button then click Secondary Antibody in the small pop up menu that will appear on the right A new screen will appear which allows you to choose a secondary data set to overlay Set the checkbox next to the sample you wish to overlay then click OK at the bottom The secondary sample will now be shown in blue on the graph along with the original orange bar To cancel the Overlay graph go back to the original click the Normal button which now appears in place of the Overlay button on the upper right Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 20 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top UNOS CPRA Calculated PRA What is the UNOS CPRA Calculated PRA CPRA is a numeric percentage score approximating the likelihood of organ transplant success or failure based on a patient s blood sample The higher the percentage the greater the likelinood of transplant failure The UNOS United Network for Organ Sharing PRA Calculator is the reference standard for all of the United States HLA Fusion communicates with UNOS over the internet but you can also run the UNOS Calculator manually through any web browser as an option within Fusion How can get the UNOS CPRA value for my current patient s within Fusion To run the UNOS CPRA calculator from within Fusion load
27. rver user name and password are used Do users need to have db owner role on the SQL Server to access and use HLA Fusion No Do I need to have Write and Modify permissions in HLA Fusion sub directories Yes How do bring back the Welcome screen in HLA Fusion software Please locate and open the HLA Fusion configuration file OneLambda Fusion Interface exe config It is located in the directory as follows For HLA Fusion IVD 4 0 C Program Files x86 One Lambda HLA Fusion 4 0 For HLA Fusion Research 4 0 C Program Files x86 One Lambda HLA Fusion Research 4 0 Locate the following line in this configuration file and change the add key value to Yes as follows lt add key WelcomeScreen value Yes gt lt add key OneLambdaEmail value gjha onelambda com gt lt add key ProductKey value D8CBEDB7 63CB Tasa 81D5 6F45B87E20DB gt lt add key SqlCommandTimeout value 1000 lt add ke ReportPath value C OLI FUSTON rpt Research 4 0 gt ey ReportDesigner value C Program Files Business Objects cryst lt add key LABXpressExePath value C LABXpr essCommander LABXpr essComman lt add key LABXpressLogPath value C OLI FUSION LABXpress gt lt add key LABXpressLabcode value 20 gt BeadPercentage value 100 gt Autocutoff value false gt Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 12 One Lambda Inc A Thermo Fisher S
28. shown Templates are stored in your Fusion system until you delete them see Step 9 7 To save the template for porting it to a different computer system press the Export button A dialog box will open asking for the location in which to save the template as a ixt file Exported templates should not be edited 8 To load a template from an exported definition file press the Import button You can then load a template that has been previously stored in txt format in Step 7 If the template s data definition exists or has been loaded previously you will be asked to choose a new name 9 To delete a template make sure it is selected in the Export Template Name box then press the Delete button How do use a Data Export Template to save data Once a Data Export Template definition is defined a customer can easily save data with it 1 From the main menu choose the Reports option then Specialty then Export Data 2 Choose the Template name in the drop down box next to the Setup button 3 Select Session or Sample data appropriate for the selected template 4 Click the Export Report button A dialog box will open offering to save the report in the location of your choice You can choose whether to save in CSV TXT or XML file format Back to the Top HLAF MAN V4 x x EN 00 Rev 0 HLA Fusion FAQ 17 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top What are the metadata options an
29. stics option under the CSV Options menu Are there any procedural differences involved with xPONENT software versions In xPONENT 3 1 After a batch is complete the CSV Output files are by default generated in a different directory from run files e Run Files C Documents and Settings All Users Application Data Luminex xPONENT31 Batch e CSV Output files C My Sessions Per session two folders with run files are generated For LABType HD analysis HLA Fusion requires the folder with the appropriate session name that ends in _rcsv These run files folders must be relocated from the default directory see above and placed into a new directory designated by the end user Only one designated directory is required to input run file folders from every session To setup the directory pathway for these relocated run file folders in HLA Fusion go to Utility gt General Settings gt Paths gt and select the run files box see image below NOTE Some of the above tasks require supervisor user privileges You may have to verify with your supervisor that these tasks have been completed Back to the Top HLAF MAN Vv4 x x EN 00 Rev 0 HLA Fusion FAQ One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top To setup the directory pathway for relocated run file folders in HLA Fusion go to Utility gt General Settings gt Paths gt and select the run files box HLA Fusion eeee8 0 Back t
30. t format How does Fusion export data and what is a Data Export Template Fusion uses Data Export templates to make exporting of data consistent and effortless Templates are defined in advance specifying the kind of data to save and in what form Templates are then used by a variety of users to save data without requiring special skills to define elaborate reporting definitions each time data is saved Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 16 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top How do create modify and delete Data Export Templates 1 From the main menu choose the Reports option then Specialty then Export Data 2 Inthe main Reporter screen Click the Setup button on the far right above the Session Samples control to bring up the Export Template Setup screen 3 To create a new template enter a name for the export template The name cannot exceed 20 alphanumeric characters To modify an existing template select the template name from the existing template drop down list 4 Select the fields that should be included in the template using the checkboxes on the left Note Opening the Options checkbox will reveal special capabilities discussed below 5 Arrange the order for data field output dragging fields up or down in the output list on the right of the dialog box 6 Click the Save button to save this template A confirmation message is
31. tates Trimmed Mean values are missing and how do resolve it How do archive some of my older data What does A_0 A_0 mean in LABType and SSP Analysis screens Why do get an error message when try to import an output file Why is the Date field yellow on the Session Import window Do archived sessions or samples appear on the Manage Data screen Does the software allow me to delete LABType IVD Only or Micro SSP data at the sample level Can perform auto analysis on import of exon 4 batch sessions Why can t go back to the session summary screen from the Micro SSP analysis window My Sero Code is a dash so why is it recorded as a blank in the Patient Results Why can t remove patient assigned results from the analysis window Why don t I see the serology value entered for a patient HLAF MAN Vv4 x x EN 00 Rev 0 HLA Fusion FAQ One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x What are the different formats allowed for sample list information Local Sample Patient ID Data Why can t view my HLA Fusion Reports Why did receive a module timed out message when tried to load samples for reporting Do archived sessions or samples appear on the Reports window Do samples excluded from analysis appear on the Reports window Why is my data missing from the sessions samples list in the Reports module How are items like sample IDs sorted in reports Why is the Report Designer Prog
32. the sample grid allows the current layout to be preserved How can we add donors to a patient from the Ab Tracking module Click on the square Patient Icon next to the Donor drop down box Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 19 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top What does maiched mismatched Ag mean Matched Antigens Antigens which are common to both patient and donor Misatched Antigens Antigens which are not common to both patient and donor What does Ab Tracking Threshold do The Ab Tracking Threshold filters out all antigens that have an MFI that is less than the threshold from the current graph plot What does the LABScreen Overlay do The LABScreen Overlay function allows the user to be able to compare MFI signals of the same sample or a sample associated to the same patient that have been tested under different test conditions like the use of a different secondary antibody User can also overlay the MFI signals of the background negative serum that is used for the normalization of the test serum How can use the LABScreen Overlay From the main menu click the LABScreen button Import a data file into Fusion if a data set is not already present in the Navigator then invoke the Navigator and click the imported data set The Session screen will now be displayed Double click on a sample from within the Session sc
33. unacceptable antigens in the appropriate data entry field then activate the CPRA Calculator From either the Patient Donor Information or Analysis screens 1 Make sure there are some Unacceptable Antigens in the data entry box of the same name 2 Make sure the CPRA button is available not greyed out 3 Press the CPRA button A percent score will appear to the immediate right of the button along with the date the percentage was calculated How can get the UNOS CPRA value using the Web Version 1 The Internet based UNOS Calculator is useful if you want to print out your settings This option only works from the Patient Donor Information HLA Tests Screen 2 Locate the Unacceptable Antigens box and make sure there are some Unacceptable Antigens in the data entry box 3 Set the checkbox just under the CPRA button Show UNOS Web Calculator then press the CPRA button 4 Aweb browser window will open at the UNOS Website You should find yourself in the UNOS Web Calculator for PRA 5 The selections that you made in the Unacceptable Antigens box are displayed The CPRA percentage score will be displayed on the screen Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 21 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top Does the Fusion software automatically update the UNOS CPRA value every time update patient assignments No The CPRA value does not auto
34. updating it from a directory on your computer or network by auto update or by going to the One Lambda OLI website How often should update NMDP Serological Equivalence and Catalog Reference Files e NMDP files should be updated according to your lab s policy e Serological Equivalence files should be updated when new nomenclature is added to catalog files and when you see _0 for serological analysis assignments e Catalog files should be updated when new nomenclature is used Does HLA Fusion accept cat and cry catalog reference files HLA Fusion accepts cat files only and no longer supports cry files which were used with an older software product that is no longer in use Why aren t the catalogs just downloaded displaying on the product home page If the current page does not show updated information upon modification or downloads go back to the main Home page and then return to the product home page to see the change How does HLA Fusion determine the most recent available catalog To determine which catalog is the most recent available HLA Fusion looks first at the lot number and then the revision number An updated lot number gets flagged as the most recent version of a catalog even if the revision number of the previous lot has been updated since you last downloaded catalogs Why didn t a column in my allele frequency file get imported If the header for the column of any allele frequency file you import
35. ust be in one of the following formats New packing list format This file gives the fields in this order ShipmentLoc 1 13 SamplelDName 0198 0398 0 SampleT ype AB DR or AB DR TurnaroundTime 14 21 or 14AB 21DR DCN 3 digit example line 1 13 0198 0398 0 AB DR 14AB 21DR 074 Pack list Old Standard X samples This file gives the fields in this order ShipmentLoc SamplelIDName SampleType 1 2 3 and an X for AB DR samples DCN example line 1 12 0287 7867 8 X 07 Old packing list format 11 for AB DR samples This file lists in this order ShipmentLoc SamplelIDName SampleType 1 2 3 and an 11 for AB DR samples DCN example line 1 15 0287 0779 2 11 074 Back to the Top HLAF MAN v4 x x EN 00 Rev 0 HLA Fusion FAQ 25 One Lambda Inc A Thermo Fisher Scientific Brand HLA Fusion Version 4 x x Back to the Top Comma delimited format Comma Separated Values CSV Each field is separated by commas The use of quotes around a field is optional and is required only if the contents of the field use a comma which could confuse field separation This file lists in this order ShipmentLoc SamplelIDName SampleType AB DR or AB DR TurnaroundTime 14 21 or 14AB 21DR DCN example line 4 12 0287 7867 8 AB DR 14AB 21DR 074 Tab delimited format Each field is separated by a tab This file lists in this order ShipmentLoc SamplelIDName SampleType AB DR or
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