Here - Orion & Saturn : you won`t recognize your SEM
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1. Orion 6 for Windows 22 5 1 1 Grab in slow scan must SYNC with SEM speed Click the following icon in the main toolbar to display the slow scan grabbing window The image sizes available for grabbing are displayed here up to 8 per scan speed Click on the tabs below to select Displays the current SEM visual scanning speed after clocking the Sync button amp Slow scan grabbing window File Options Help Hable formats M Acquisition full REM screen 2 640 x 510 o Es wo Click here to start grabbing only when system is SYNC ed eeeeween Selects the grabbing method direct slow or fast averaging or frame integration from 2 to 256 frames Click here to first synchronize the Orion board with the SEM scanning speed This selects the set of max 8 image sizes to grab The same window is displayed below when the AutoSync feature is enabled In this case the Orion system detects any visual speed change and updates the useable grabbing formats on the left To activate deactivate the AutoSync feature go in the main toolbar to Options Preferences Miscellaneous Select the second page called Grabbing Select Slow scan grabbing window type 1 and select the automatic sync method refer to the previous chapter for the corresponding screen dump Grabbing slow scan mode BBE File Options Help m Image description Acquisit2. ccccccecccsscesscesccssccesccscccescsssssseeseseccesscsscssscnsccnsecaaecaaseaaveaeeeaevesessseessesseeaees 14 3 5 PESYSTEM REQUIREMENTS 3 c 0i2cc 8 ceri nan diese hie re ceeded nnd ant M da as 14 4 THE ORION MAIN TOOLBAR 0 cece cece cece cece cece cecececeseeseseeces 15 4 1 RUNNING THE ORION SYSTEM cccccecccsscscseecceecceescesscesccsccsccsccaascaesesevsaevessvscessccesscsscssecnaeceaecaaecaseaesenseensesenes 15 4 2 DESCRIPTION OF THE MAIN MENU u ccceccesccesccesccecccscccecccsscceceecccesccscceaccnsecsaecaascasvessveaeeesevesesecesssesecaccnacenaes 15 42I ECON OSC DONS ions asie ones 16 4 2 2 Structure of the drop down menu of the main toolbar 17 4 3 MAIN TOOLBAR ORGANIZATION OF THE BASIC VERSION c ccccccsscsscescssccssesecesesecssesscsscssecssesscsssssesseessenecsueass 19 5 ACQUIRING AN IMAGE 0 ee eee ec eee e ence cece cee eseseesesseseseesesees 20 5 1 HOW TO ACQUIRE AN IMAGE IN SLOW SCAN VISUAL RECURRENT MODE cccccsccscscscsesseseeeececseseeaees 20 5 11 Grab in slow scan must SYNC with SEM speed nn 22 5 1 3 Grabbing in photo record MOOESS nn nement adrien 24 5 1 4 Commands available during grabbing us 25 S MALT In visual modes only lire nn Donne den Rss ii sims ner et life LE 25 91 42 irecord MODES OAV enr nn E meer msn dns annee Re nee den eee nn es emmen nee ne E Een ent Ste 26 6 AFTER GRABBING eee eee ce cece ce
3. Select another system user Options Orion 6 for Windows 19 Language gt English French Preferences Printer setup Setup Resolutions Change default image resolution number of DPI Main toolbar layout button size Miscellaneous Display tips at startup System setup Orion technical configuration Create system backup Install system options Adjust your screen X Y ratio Help on Orion system on grabbing on macros How to upgrade About version info and how to contact us 4 3 Main toolbar organization of the basic version The active buttons and drop down menu commands are file open file save grabbing in visual mode grabbing in photo mode and zoom display To interpolate the image pixels go to File Interpolate in the image window When you need to upgrade to the full level go to Options System setup Install system options and open the option file you can get from your system installer or ELI Belgium our email address is Sales OrionMicroscopy com Orion 6 for Windows 20 5 Acquiring an image To acquire an image from the SEM the Orion system synchronizes with the SEM scanning circuitry and reads the analog video signal produced by the SEM A copy of the image is stored in memory while it is displayed during grabbing The digitizing process requires that the SEM contrast and brightness first be adjusted on the SEM before gr
4. the selected color The option includes another unique function for packing high size mappings and retrieve a higher dot density in only one scan in photo mode The scanning must be provided by the SEM circuitry no active drive from EDX The option includes the following tools e Frame integration of EDX dot maps You can collect dot maps by integrating several frames in order to get a higher density dot map at the end The resulting mapping has a black background with white dots e Mixing up to 16 mappings into an SE or BSE image The result is a gray scale image with colored dots one color per mapping e A dot map packing utility This produces mappings with a higher dot density e A threshold function This delivers white dots on a black background even if the contrast or brightness is not ideal during grabbing The following page shows the result of such a process Orion 6 for Windows Step 1 acquire an SE or BSE image of your sample EF Img 01 EdxSE jpg 640x480 pixels 101 2451109 alb XIMO R M not calibrated 640x480x8 bpp Step 2 acquire a dot map of the same area EF Img 02 EDXCu jpg 640x480 pixels 101 11 170 4770 CDX ORM not calibrated Step 3 mix the two images together EM Img 01 EdxSE jpg 640x480 pixels 101 Palette Tools EDX 3D Help 17 0 102 MED XML Or IE not calibrated 640x480x8 bpp 640x480x8 bpp Orion 6 for Windows 12
5. 3 System description 3 1 Software organization and system users The main menu displays all the global system commands Some of them are accessible both from the drop down menu and from a dedicated button like for example the grabbing commands Many others which are specific to the selected image are also available from the drop down menu in the image window or by right clicking in the image When an image is acquired from the SEM it is automatically stored in computer memory and is immediately available for storage pasting to clipboard filtering All popular files formats are available TIF BMP SUN raster JPEG compressed and many others the future image use will usually define which image format has to be used for storage For example when image processing is required after storage an uncompressed format must be used because the compression decompression algorithm modifies the pixel value 3 2 Description of the grabbing process The grabbing process transforms the analog video signal produced by the SEM into an array of gray levels that represents the image Depending on the scanning mode photo or visual the system synchronizes differently but the image is in both cases stored in the PC RAM memory and is immediately available after grabbing During the grabbing process the system is synchronized with the SEM scanning and tracks every line produced by the SEM the pixels of the current line are displayed averaged if r
6. 600 x 1200 pixels only any number of colors the best setting is 16 7 Mcolors in order to get 256 true gray levels on screen e agraphic board with min 8 MB is required with PCI or better AGP bus interface e a high capacity hard drive Orion 6 for Windows 15 4 The Orion main toolbar This chapter describes how to start Orion and the main menu It explains the functions attached to the main menu icons and the data displayed in the main menu 4 1 Running the Orion system The following icon has been created during the installation process and has to be double clicked in order to start Orion m Once started the main menu will be displayed at its latest position used before or at the top of the screen depending on the startup option described later 4 2 Description of the main menu The main menu is divided into three parts e the drop down main menu functions can be called by clicking the desired text and dropping the mouse down to display the corresponding list of sub functions e the icons they are shortcuts of commands of the main menu they can be programmed at will with the macro InitButton These two buttons open the grabbing utilities slow scan and photo modes Graphic tools drawings overlays extraction See 8 Display the overlay See 3 manager See 9 Save the current image on drive See 2 Display the Orion 6 60 3 in Evaluation Mode calibration list File Edit SEM ma
7. E L I sprl Orion version 6 High Resolution 32 bits PCI Image Grabbing System For Windows 2000 XP and Seven User s Manual Part 1 Description of the Basic Package CZ DSM Macro Remote Contra Commands opt module opt module lt nn Dress EDX dot ma Graphic amp acquisition Tools Kort modi opt module Su d 3D visio opt module wa Release 66 February 1 2010 E L I sprl Belgium Orion 6 for Windows 2 INDEX 1 INTRODUCTION 2 225 225 peo dress insider dla der teste died ainntin denned 4 2 OPTIONAL SOFTWARE MODULES sseeseseeeeseseeseseeseseeeeeeeeeeenee 6 LL GRAPHIC TOOLS 2 nn tn CN ose thd Re aoe oes Bo etree Aaah ah ions Ci 7 LE MACRO COMMANDS 338 ee ns nn Oe i de rude nat Men Ut nt tt en a ale rare 8 AINA GUY PIAS ta RER RS Mme A ne est Na Sn CAN a oe 9 24 RS 232 FOR LEO DSM ide ce ine eee ee Lien a en aa 10 215 EDX MWAPRPENG MIXER EE E ee ee cree eee tos Aen eet eae nm erie tte ne 10 3 SYSTEM DESCRIPTION 0 eee eee cece cece e cess eseseeseseeseseesesees 12 3 1 SOFTWARE ORGANIZATION AND SYSTEM USERS ccccescesceccescsecssccscvsccesvcceacesessccesessceseaccasesccaevscenaveneeaceateacens 12 3 2 DESCRIPTION OF THE GRABBING PROCESS cccccsccesscesccscccsccesscesseesceescesscesscssecusccusecaaesaaseaeseaeeeseseseusenssenssenaees 12 3 3 SEM SCANNING CHARACTERISTICS AND DRIVER FILE sise 13 3 4 ADDITIONAL SYSTEM FUNCTIONS
8. abbing i e the Orion board contrast and brightness is set during the installation to be the same as your photo record tube Paragraph 5 1 4 is important it shows which commands are available during grabbing a 1 5 1 How to acquire an image in slow scan visual recurrent mode The system can display three grabbing windows In slow scan modes Type 1 a window for grabbing image in slow scan visual or recurrent mode only Type 2 a window for grabbing in photo modes only all SEMs except DSMs with RS232 see par 3 1 3 Type 3 a window specifically designed for the LEO DSMs series with the RS232 connection see par 3 1 4 If you are connected to a LEO DSM with an RS232 connection the best choice is to use type 3 because this allows the system to automatically interrogate the DSM after grabbing and get the micron bar information for the new image Orion 6 for Windows 21 To select the window type in the main toolbar go to Options Preferences This displays the following utility Page 1 General This allows you to set the scale bar parameters when the system automatically generates a micron bar in an extracted image and when the mother image is calibrated This value influences the file compression ratio the effective compression ratio depends on the image details noise Page 2 Grabbing This setting is used during grabbing It forces the system to repla
9. act us We are at your disposal for more information on sales and support E L I sprl sales services Rue Gaston Dancot 55 B 5310 Eghezee Belgium Tel 32 81 512008 Fax 32 71 302 312 Email Paul OrionMicroscopy com E L I sprl technical services Rue du Vieux Chateau 17 B 6567 Labuissi re Belgium Tel 32 71 30 98 17 Fax 32 71 302 312 Email support OrionMicroscopy com Visit our WEB site http www OrionMicroscopy com
10. by using the SelectFolder SaveImage macro commands There are a number of available formats for saving the image on disk Select the right format in relation with your future image use TIFF BMP for general use JPG or CMP for archiving with compression SUN raster format when the image needs to be transferred to a SUN SPARC workstation The supported formats are 1 8 9 LEAD CMP compress format It delivers a much smaller file size and better image quality than other compression techniques including JPEG JFIF JPEG File Interchange Format with full color spacing YUV 4 4 4 JFIF JPEG File Interchange Format with YUV 4 2 2 color spacing to produce a smaller JFIF file JFIF JPEG File Interchange Format with YUV 4 1 1 color spacing to produce the smallest JFIF file JTIF JPEG Tagged Interchange Format with full color spacing YUV 4 4 4 JFIF JPEG Tagged Interchange Format with YUV 4 2 2 color spacing to produce a smaller JTIF file JFIF JPEG Tagged Interchange Format with YUV 4 1 1 color spacing to produce the smallest JTIF file TrueVision TGA Targa uncompressed format created by TrueVision Inc TIFF This is a tag based field format designed to promote universal interchange of digital image data 10 Windows Bitmaps BMP This is a file format created for Microsoft Windows Some BMP images are compressed with an RLE type compression 11 SUN Raster Created and used by SUN on the wor
11. ce cece ceseecuseecussecusesaesees 27 6 1 HOW TO GET ACCESS TO THE AVAILABLE COMMANDS c cccccccscsssesscssesscsscescsscesssecesesscssessevscssessecesesecsuesseneees 27 6 2 DESCRIPTION OF THE IMAGE WINDOW DROP DOWN MENU WHEN AVAILABLE s ssisssssisssississerrrsessesesee 28 6 3 LET S SAVE THE TMAGEON DISK asia aa nine hie Babee R GIE ART E aE a a 29 6 4 TRANSFER IT TO ANOTHER APPLICATION THROUGH THE CLIPBOARD mener eee 30 6 5 CHANGING THE DEFAULT IMAGE SIZE ON SCREEN AFTER ACQUISITION c cccccsceesessscecececsesenseaececccecsesesaeceeceeeeensaaeas 30 TR HOME TO CONTACT US EE Moe a dat TURN DORE PARA Catt Mira aie aw PSS CPR CEE 31 Orion 6 for Windows Preliminary notes The Orion system is a real time grabbing system This means that it has to track the scanning of your SEM and catch the lines and pixels as they are generated by the SEM It s not recommended to run any other program while grabbing as these jobs use processing time and thus can prevent the Orion system to function correctly Please turn off your screen saver as it can interfere with this software The stand by mode of the PC has also to be turned off Orion 6 for Windows 4 1 Introduction The PCI design uses Plug and Play features for allocating memory spaces and the IRQ channel used by the board The Orion software consists in two levels The BASE version offers the following functions e image grabbing in slow scan visual mode
12. ce the extreme gray levels with colors for a better appreciation of the image contrast and brightness This changes the grabbing window displayed when you click the SEM button in the main toolbar third from left Some options are SEM specific and linked to the technical configuration General Grabbing gt User must confirm M image erases F macro script files saves updates gt Image windows display drop down menu FN save image data with image file I close free drawing loops gt JPEG Compression Bj gt Quality factor 40 m Micron bar ion in extracted area r Text C opaque black background C transparent semi transparent Location at the top C at the bottom M Type amp within the created image C outside the image area User options Slow scan grabbing window type 1 with syne button type 2 with image sizes list C type 3 universal visual photo S type 4 specific to ISI SX40 C type 5 specific to ABT 32 60 Topcon 510 C type 6 specific to LEO 250 C type 7 specific to LEO 90 100 120 200 C type 8 specific to Amray 1830 SEM sync method automatic C manual with SYNC button This new option is displayed only when grabbing window type 1 is selected It allows you to let the system track the SEM scanning speed automatically or to do it yourself by clicking the Sync button
13. ds to see the depth of his sample This 3D extension mixes stereo images i e grabbed when sample is tilted by 5 10 degrees The two images are mixed and the result is red green or red blue coded As with the EDX extension the resulting image has 24 bits per pixel and can be printed in colors It also includes a tool for semi automatic image alignment With this utility you can compensate small stage drift during tilt Here is an example of the difference between a normal view and a stereo view special red green or red blue pair of glasses is required Orion 6 for Windows 10 2 4 RS 232 for LEO DSM This option allows you to digitize images from the DSM and reconstruct the correct micron bar information in the image by interrogating the DSM through the RS232 interface You can remote start the photo mode or any slow scan mode from the computer Because of the design of the DSM the Orion system must interrogate the DSM right after grabbing in order to reconstruct the micron bar information The final image has the same design as the original DSM image 2 5 EDX mapping mixer This utility allows you to grab an EDX dot map in photo mode or by recursively collecting frames in visual mode this enhances the dot density in the resulting image You can also mix an EDX dot map white dots on black background with any gray scale image of the same size The resulting image displays the original gray scale image PLUS the dot map in
14. e ultra high resolution offered by Orion during grabbing 3 3 SEM scanning characteristics and driver file As every existing SEM has its own scanning characteristics number of lines screen form factor and line time it is required to adapt the grabbing characteristics to each scan mode of every SEM This is done by checking the SEM scanning characteristics during the installation process A SEM driver file stores all these parameters In the Orion system all driver files are located in a special folder drivers and have the SEM extension A driver file defines up to 72 different image sizes with maximum 8 sizes per visual scanning speed i e for each SEM scanning speed and 8 different scanning speeds The driver also stores information for up to eight image sizes in photo modes But the number of really definable image sizes is in fact a function of the scanning capabilities of the SEM Everything is explained in the technical manual which is reserved to the installers trained by E L I Belgium The whole system is designed to deliver images with square pixels The sensitivity of your Orion board i e contrast and brightness has been set up during the installation phase by a trained engineer who is responsible for the installation Usually the board sensitivity is adjusted in order to match the one of the photo device So the adjustments for grabbing an image are the same as when taking a picture The grabbing proce
15. equired and stored in memory in real time i e at the time the line is scanned by the SEM The photo or record modes are generally defined by a slower scan speed i e a higher number of ms per line between 10 and 100 ms or more a higher number of scanning lines it can be several thousands usually between 1000 and 4000 and there is only one scan the resulting image contains less noise and has a higher resolution The slow scan or visual modes are defined by a faster scan speed usually between 1 and 15 ms and a lower number of lines from 256 to 1000 but the scanning process is endless which allows for example frame integration or averaging for noise reduction The ultra high resolution of Orion for Windows allows transparent pixel oversampling during grabbing this means that the Orion card can take several measures of the same sample area during the grabbing process This feature is made in real time and does not Orion 6 for Windows 13 cause any delay in the grabbing process The big advantage is that the amplitude of the noise in the image is reduced by the square root of the oversampling factor It is a good practice to digitize the biggest possible image i e with the highest possible number of pixels and lines because image size reduction with interpolated pixels will give you noise free and very high quality images After reduction you can close the original image to gain memory space This is possible because of th
16. eset palette to linear Apply palette to pixels Open palette file Savve palette Overlay Manager Show Calibrations Show Hide Overlays Delete all overlays Mix EDX mapping w image pack mapping Threshold dots levels Mark reference location Align image from reference Combine views Description creates an interpolated image calibrates the pixel size rotates flip current image saves the selected image on disk displays technical information forces a clean display of the image Creates a copy of the image makes a hardcopy of the bitmap closes current image window modifies palette or pixel values displays red blue palette limits displays linear palette modifies pixel values from palette loads a color palette from disk saves current image palette to disk displays the Overlay Manager displays the list of known pixel sizes permanently removes all overlays mixes the with an EDX mapping reduce size of mapping by 2 makes a thresholding sets aref point for alignment makes the alignment creates the anaglyph from 2 views Displays help Orion 6 for Windows 29 6 3 Let s save the image on disk The image save function can be called the four following ways by clicking the corresponding con in the main menu by selecting the File Save command in the main menu by right clicking with the mouse in the image window and selecting the File Save command see paragraph 4 2 here above
17. ge Gallery Macros Settipgs Help See 9 Email the image See 11 Copy the image Open an into the image file clipboard See 1 See 5 Display the annotation utility See 10 Display the image Display the magnifier zoom macro editor See 7 See 6 Orion 6 for Windows 16 4 2 1 Icons description 1 Saves an image on disk The image that will be saved is the selected image its window caption is displayed in the bottom line of the main menu it is the last one you have clicked in 2 Loads an image from disk The image to load will be displayed in a separate independent window 3 Displays the slow scan grabbing window 4 Displays the photo mode grabbing window 5 Copies the image to the clipboard 6 This button displays the macro command editor when the option is installed If the option is not available then this button is gray and inactive The macro command editor is a text editor optimized for editing CMD files and executing macro commands step by step 7 This button displays a zoom window that displays the area around the cursor position Depending on the zoom settings an area of 64x64 128x128 or 256x256 pixels is magnified The size of the zoomed area changes the magnification factor You can also resize the zoom window by dragging its lower right corner 8 This one displays what we are calling the Graphic Tools utility This is a set of functions which are now easily accessible through
18. ge that will be processed and its name is displayed in the main Orion menu When you have many images opened at the same time it becomes interesting either to show them all on the screen or to reduce them all to icons in order to clear the screen These commands are available from the main menu see paragraph 2 2 2 above Many of the image functions can be accessed different ways from the main drop down menu from the icons of the main toolbar from the popup menu available in the image window when you right click with the mouse or from the image drop down menu 6 1 How to get access to the available commands There are basically four ways to get access to the image commands e from the main menu drop down text menu e from the icons in the main menu e by right clicking in the image window with the mouse e From the image drop down menu The selected command will apply to the currently selected image because clicking in the window makes that image the selected current one When you select a command from the main menu the target image is the one whose caption bar is highlighted Orion 6 for Windows 28 6 2 Description of the image window drop down menu when available File Commands Interpolate pixels Calibrate Rotate Flip Save Info Recalibrate display Duplicate Print Close Palette Tools EDX 3D Edit contrast brightness Show saturations R
19. guration for each user you can create new buttons that will execute your specific sequence of actions This is done by selecting a user at startup from a list Each user in the list is linked to a macro command initialization file This file is executed when the user is selected The macro file can include functions like changing the main menu initializing buttons with automatic grabbing and printing functions or even selecting the default folder where all users images will be saved The macro command option is required to use this feature because the user selection menu can be called only if the macro function has been enabled The Options handbook fully describes these commands with extensive examples and the way to implement them In the following example two screen dumps were made of the main tool bar the default toolbar amp Orion 6 60 3 demo mode z oj x File Edit SEM Image Gallery Macros Settings Help the same toolbar in which the user added two new buttons with his code in this case one for grabbing in record mode and automatically resample the image right after grabbing and the second one for extracting an area of a constant size here 800x600 pixels Orion 6 60 3 demo mode Img 01 EdxSEjpg 6405480 pin File Edit SEM Image Gallery Macros Settings Help amp am dals als a slels 2 x Orion 6 for Windows 9 2 3 Anaglyphs This option is required when the user nee
20. ion Computed format 656 x 510 Direct refresh z as medium ok X Quit 2eeeenee Orion 6 for Windows 23 This window displays the image formats that are currently available for the current scanning speed In the first version clicking the SYNC button checks the SEM speed When this button is pressed the SEM scanning speed is measured and compared with the contents of the driver file If the current speed was programmed in the SEM driver then the available image sizes for this speed will be displayed on the left and the line time displayed in a green area Selected the appropriate image size in the left list If the scanning speed was not set up no sync is displayed in a red area Other parameters have to be set before grabbing They are e the video input used for grabbing this selection is useful only when the 2 inputs are wired to the SEM The default is input number 1 e the grabbing method used direct refresh slow and fast averaging or frame integration If the area is green click OK to start grabbing The grabbing process first creates a blank window on screen then synchronizes with the SEM scanning and fills the displayed window with the live SEM image Orion 6 for Windows 24 5 1 3 Grabbing in photo record modes First make any necessary adjustment on the SEM in order to get a correct image contrast brightness astigmatism In particular adjust brightness and c
21. ions EDX dot ma acquisiTiog Graphic Tools l opt moduli Orion 6 for Windows 7 2 1 Graphic Tools This option gives access to several very useful functions that will help you handling and evaluating your images after grabbing Printing with preview capability Distance measurement line segments overplayed or burned in pixel layer Image annotation Area extraction Brightness contrast adjustment after grabbing Image resize Palette processing and histogram analysis Automatic Micron bar creation in extracted images Email capability right after grabbing This an example of the automatic scale bar generation in an extracted area image from which the extraction was made 148 28 103 XO R W cal 4 50 pm x 4 00 k 2832x1930x8 bpp extracted area 0840096 MCD xa RAM col s x 4 004 Orion 6 for Windows 8 2 2 Macro commands Orion includes a powerful optional macro command editor and processor This extension allows you create and or change the function of 30 buttons 3 rows of 10 in the main toolbar to automatically execute macro command files Powerful commands also save and restore work variables to disk It is possible to automate grabbing tasks by using the GrabSlowScan or GrabPhoto commands and tell the system to automatically execute image filtering commands after grabbing This option is a new way of optimizing the Orion system confi
22. kstations Orion 6 for Windows 30 6 4 Transfer it to another application through the clipboard It is often very useful to transfer an image between applications There are four ways to copy the image to the clipboard 1 by right clicking in the image window and selecting File Copy to clipboard 2 by selecting Edit Copy to clipboard in the main menu 3 by clicking the Copy button in the main toolbar 4 by using the ToClipboard macro command After execution of the command the file becomes available in the Windows clipboard to any other application that accepts images Note In some situations it is necessary to consider the image size For example a 2048 x 2048 pixels image takes 4 Mbytes and it can require some time to copy to the clipboard and load it in Microsoft Word 6 or any other word processing package Such a density of pixels is not necessary in this case It can slow the system down 6 5 Changing the default image size on screen after acquisition By default the Orion software uses a fixed ratio between the bitmap size and your desktop resolution in order to avoid aliaising effects and visual artefacts You can change this by going to Settings Preferences Miscellaneous Select the After Acquisition page For example choosing 50 will cause the largest border of the window either horizontal or vertical to be 50 of the width or height of the desktop Orion 6 for Windows 31 7 How to cont
23. l and displays specific menus for controlling the grabbing process In visual mode the system displays this window Selects the frame acquisition method frame integration must be selected before grabbing Visual modes gt Acquisition cbassbenseasbonnsons Activate deactivate saturation display in false colors ff Nir fast averaging slow averaging Aborts grabbing and trashes the M display false colors image M frame no 210 Stops grabbing leave the image on Stop now screen a End scan and save Stops at the end of the current scan Terminates current scan and automatically enters the file save utility File name counter is automatically updated Orion 6 for Windows 26 5 1 4 2 In record modes only Like for the visual modes the Orion system uses full mouse control and displays a specific menu for controlling the grabbing process In record mode the system displays this window Activates deactivates display of white and black in false colors W nen cherked causes Too M display false colors the system to save when finished automatically save the image after grabbing Trash it Stops grabbing v Stop now detente Se oe the image Stops grabbing The image stays on screen Orion 6 for Windows 27 6 After grabbing The images are stored in PC RAM memory Only one image is active at a time and the selected image is the active ima
24. mage Open load an image from disk Save save an image on disk Save As saves an image under a name to specify Create folder create a new directory Dump desktop copy screen to clipboard Printer setup modify printer characteristics Print Exit quit the application Copy to clipboard Paste from clipboard Duplicate make a copy of selected image Modify brightness contrast Resize image Digitize in slow scan mode Digitize in photo mode Orion 6 for Windows 18 Image Interpolate pixels create a new image with interpolated pixels Annotate write text in image Calibrate define a reference distance Extract area create a new image from an extracted area Mark extraction area write a rectangle around the extracted area Infos display technical information Linear palette modify the image s palette of grey levels Drawing color set the color used for overlaying data Yellow Red l gt Q 9 i 3 Q Overlays Delete all Display list Gallery Reorganize the screen Iconize all images reduce them all at the bottom of the screen Close all images Macros Display Fast Executor execute only one line at a time Display macro editor with full editing capabilities Display macro options Startup macro file file to execute at startup Users Select auser at startup
25. n visual mode 16 bits 2 bytes are allocated per pixel in order to maintain full pixel gray level resolution even when 256 frames have to be integrated in this case the pixel depth is reset to 8 bits after grabbing Orion 6 for Windows 5 When an image is calibrated i e when a reference distance has been manually defined or selected from the internal list your Orion system has the ability of constructing a new micron bar in the extracted image this is very useful because usually the calibration information is lost when extracting an area Please refer also to our Internet site for downloading new updates of the Orion package http www OrionMicroscopy com Minor changes can be made to the Orion package in order to enhance it This manual will be updated accordingly when required Orion 6 for Windows 6 2 Optional software modules In order to optimize the system to the user requirements we have decided to distribute the system functions between the Basic system and optional modules You can start with the base version and upgrade it with any option any time or ask for a limited time of evaluation of other options It is required to have a special initialization file to unlock the options This file is available from your system installer or ELI Belgium support OrionMicroscopy com Please refer to your Orion system installer or email us at support OrionMicroscopy com for any information you could require on these opt
26. ontrast as for taking a photo the Orion card sensitivity should have been adjusted to be the same as your photo device Click the fourth icon from the left in the main toolbar to display the photo mode grabbing window please refer to par 4 2 to locate it The following window will then either be displayed or activated if iconized the example below is valid for JEOLs 6300 6400 and 6340 SEMs M Digitize in photo mode File Options Help Image description 1 C mode P1 1280 x 1060 pixels C mode P2 2560 x 2120 pixels C mode P3 1280 x 1060 pixels C mode P4 2560 x 2120 pixels mode PS 2560 x 2120 pixels c c c This window displays the image formats that are available for grabbing in the PHOTO modes The SEM speed is not checked before grabbing so it is your responsibility to select an image size that matches the photo mode you will start at the SEM A set of up to 8 image sizes is available in the left list Select the appropriate image size and click OK to start grabbing The grabbing process first creates a blank window on screen then synchronizes with the SEM scanning and fills the displayed window with the live SEM image Wait for initialization process to finish you get a black rectangle in the grabbing window then push the photo button on your SEM Orion 6 for Windows 25 5 1 4 Commands available during grabbing 5 1 4 1 In visual modes only The Orion uses full mouse contro
27. s and photo modes e image details zooming e pixel interpolation for noise reduction e image save with access to all popular formats The Modules they allow the customer to add specific functions corresponding to his specific requirements There are 5 different modules e Graphic tools e Macro commands e EDX mappings e 3D Vision Anaglyphs e RS 232 remote control for DSM To make a module available just give us your board serial number when ordering it This key file size is only 1kbytes unlocks the purchased options Please refer to chapter 2 for more details Orion 6 has been created to be fully 32 bits compliant and compatible with Win 95 98 Me 2000 and XP The board design is radically different from the ISA design in that it has a local coprocessor uses hardware pixel integration and uses interrupts The layout of the user interface has been optimized by reducing the number of mouse clicks and the technical setup has been enhanced to provide the installer more comfort and possibilities in optimizing the grabbing characteristics During grabbing you can now use the mouse instead of the function keys Any image size from 16 x 16 up to 8 192 x 8 192 pixels is available depending on the scanning characteristics which limit the size of the image you can get from your SEM Up to 64 different image sizes can be defined in a SEM driver Images are grabbed and stored with 256 gray levels per pixel When integrating frames i
28. small shortcut icons at the bottom of each image window This button is active only if the corresponding optional module has been installed and is available in your system 9 The Overlay Manager allows you to hide show or delete image overlays An overlay can be a rectangle a line of a free drawing that you draw on the image The overlay color can be selected either through the Graphic Tools utility or by hitting the O icon in blue at the bottom of the image Black or white colors will let you burn the line in the image pixels while any other color will let you draw the next line as an overlay i e as a part of your screen rather than a part of the image pixels Orion 6 for Windows 17 10 The annotation utility allows you to write a one line text in the image pixels in black or white You can choose different sizes and use a text background or not If you push le left button of your mouse and drag it in the image the system draws a line between the text and the subject in the image 11 With this utility you can email an image right after grabbing This is a simple way of sending a document you don t have to save the image and spend time retrieving it from your drive 12 This button displays the main help window 13 This button closes the Orion application 4 2 2 Structure of the drop down menu of the main toolbar The base version includes only the command marked with an File New create a new blank i
29. ss does not interfere with your SEM photo device Orion 6 for Windows 14 3 4 Additional system functions The Graphic Tools optional module also includes a number of functions in direct relation with the work at the SEM e image filtering low and high pass median Sobel contrast equalization e image sizes reduction with interpolated pixels e image extraction Orion creates a new micron bar in the extracted image e image inclusion for mosaic creation e Option to create a micron marker for example when connected to a LEO DSM e draw colored texts lines and rectangles in an image as overlays or buried in pixels e compress images on disk CMP optimized JPEG JPEG JTIF JFIF formats are available e printing e during grabbing the user can use the mouse for changing acquisition parameters Additional menus are displayed while grabbing 3 5 PC system requirements Since grabbing is essentially a real time process SEM scanning data must be grabbed and stored as they are produced by the scanning otherwise they are lost it is required to fulfill the following minimum requirements e the processor must be at least a Pentium 500 MHz with 128 Mbytes RAM 512 MB are recommended specially with 2000 or XP e MS Windows R version 95 98 Me 2000 or XP e A full length PCI slot the PCI board measures 260x100 mm excluding the PCI connector e supported Windows graphic modes are 1024 x 768 1152x864 1280x1024 and 1
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